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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 334 |
| AccII | CGCG | 3 cut(s) | 50, 66, 242 |
| AclWI | GGATC | 2 cut(s) | 177, 558 |
| AcoI | YGGCCR | 2 cut(s) | 237, 282 |
| AdeI | CACNNNGTG | 1 cut(s) | 305 |
| AfaI | GTAC | 3 cut(s) | 152, 372, 384 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 53, 344, 495 |
| AgsI | TTSAA | 2 cut(s) | 484, 590 |
| AjnI | CCWGG | 5 cut(s) | 109, 323, 358, 397, 488 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 260, 292 |
| AlwI | GGATC | 2 cut(s) | 177, 558 |
| AoxI | GGCC | 5 cut(s) | 130, 216, 237, 282, 362 |
| ApeKI | GCWGC | 3 cut(s) | 114, 117, 318 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 462 |
| AspLEI | GCGC | 3 cut(s) | 68, 207, 244 |
| AspS9I | GGNCC | 3 cut(s) | 19, 362, 416 |
| AsuC2I | CCSGG | 2 cut(s) | 93, 296 |
| AsuHPI | GGTGA | 3 cut(s) | 110, 136, 590 |
| AvaII | GGWCC | 2 cut(s) | 19, 416 |
| BbvI | GCAGC | 3 cut(s) | 126, 129, 330 |
| BccI | CCATC | 1 cut(s) | 229 |
| BceAI | ACGGC | 1 cut(s) | 361 |
| BciT130I | CCWGG | 5 cut(s) | 111, 325, 360, 399, 490 |
| BcnI | CCSGG | 2 cut(s) | 93, 296 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 129 |
| BglII | AGATCT | 1 cut(s) | 136 |
| BisI | GCNGC | 6 cut(s) | 115, 118, 121, 240, 282, 319 |
| BlsI | GCNGC | 6 cut(s) | 116, 119, 122, 241, 283, 320 |
| Bme1390I | CCNGG | 7 cut(s) | 93, 111, 296, 325, 360, 399, 490 |
| Bme18I | GGWCC | 2 cut(s) | 19, 416 |
| BmgT120I | GGNCC | 3 cut(s) | 19, 362, 416 |
| BmiI | GGNNCC | 3 cut(s) | 20, 21, 417 |
| BmrFI | CCNGG | 7 cut(s) | 93, 111, 296, 325, 360, 399, 490 |
| BmsI | GCATC | 1 cut(s) | 345 |
| BpuMI | CCSGG | 2 cut(s) | 93, 296 |
| BsaJI | CCNNGG | 4 cut(s) | 91, 359, 398, 489 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 53, 344, 495 |
| Bse118I | RCCGGY | 1 cut(s) | 364 |
| Bse1I | ACTGG | 1 cut(s) | 168 |
| BseBI | CCWGG | 5 cut(s) | 111, 325, 360, 399, 490 |
| BseDI | CCNNGG | 4 cut(s) | 91, 359, 398, 489 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 53, 344, 495 |
| BseMII | CTCAG | 1 cut(s) | 492 |
| BseNI | ACTGG | 1 cut(s) | 168 |
| BseRI | GAGGAG | 2 cut(s) | 212, 215 |
| BseXI | GCAGC | 3 cut(s) | 126, 129, 330 |
| BseYI | CCCAGC | 1 cut(s) | 338 |
| Bsh1236I | CGCG | 3 cut(s) | 50, 66, 242 |
| Bsh1285I | CGRYCG | 3 cut(s) | 54, 292, 369 |
| BshFI | GGCC | 5 cut(s) | 132, 218, 239, 284, 364 |
| BsiEI | CGRYCG | 3 cut(s) | 54, 292, 369 |
| BsiSI | CCGG | 3 cut(s) | 93, 296, 365 |
| BslFI | GGGAC | 2 cut(s) | 32, 429 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 53, 344, 495 |
| BsmFI | GGGAC | 2 cut(s) | 32, 429 |
| BsmI | GAATGC | 1 cut(s) | 490 |
| BsnI | GGCC | 5 cut(s) | 132, 218, 239, 284, 364 |
| Bsp143I | GATC | 4 cut(s) | 51, 136, 169, 563 |
| BspANI | GGCC | 5 cut(s) | 132, 218, 239, 284, 364 |
| BspCNI | CTCAG | 1 cut(s) | 493 |
| BspFNI | CGCG | 3 cut(s) | 50, 66, 242 |
| BspHI | TCATGA | 1 cut(s) | 246 |
| BspLI | GGNNCC | 3 cut(s) | 20, 21, 417 |
| BspPI | GGATC | 2 cut(s) | 177, 558 |
| BsrFI | RCCGGY | 1 cut(s) | 364 |
| BsrI | ACTGG | 1 cut(s) | 168 |
| BssAI | RCCGGY | 1 cut(s) | 364 |
| BssECI | CCNNGG | 4 cut(s) | 91, 359, 398, 489 |
| BssMI | GATC | 4 cut(s) | 51, 136, 169, 563 |
| Bst2UI | CCWGG | 5 cut(s) | 111, 325, 360, 399, 490 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstDEI | CTNAG | 1 cut(s) | 501 |
| BstEII | GGTNACC | 1 cut(s) | 596 |
| BstFNI | CGCG | 3 cut(s) | 50, 66, 242 |
| BstHHI | GCGC | 3 cut(s) | 68, 207, 244 |
| BstKTI | GATC | 4 cut(s) | 54, 139, 172, 566 |
| BstMBI | GATC | 4 cut(s) | 51, 136, 169, 563 |
| BstMCI | CGRYCG | 3 cut(s) | 54, 292, 369 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 63, 120, 129, 385 |
| BstNI | CCWGG | 5 cut(s) | 111, 325, 360, 399, 490 |
| BstNSI | RCATGY | 1 cut(s) | 383 |
| BstPI | GGTNACC | 1 cut(s) | 596 |
| BstSCI | CCNGG | 7 cut(s) | 91, 109, 294, 323, 358, 397, 488 |
| BstUI | CGCG | 3 cut(s) | 50, 66, 242 |
| BstV1I | GCAGC | 3 cut(s) | 126, 129, 330 |
| BstX2I | RGATCY | 2 cut(s) | 136, 169 |
| BstYI | RGATCY | 2 cut(s) | 136, 169 |
| BsuRI | GGCC | 5 cut(s) | 132, 218, 239, 284, 364 |
| BtsIMutI | CAGTG | 1 cut(s) | 161 |
| CciI | TCATGA | 1 cut(s) | 246 |
| CfoI | GCGC | 3 cut(s) | 68, 207, 244 |
| Cfr10I | RCCGGY | 1 cut(s) | 364 |
| Cfr13I | GGNCC | 3 cut(s) | 19, 362, 416 |
| Csp6I | GTAC | 3 cut(s) | 151, 371, 383 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 384, 419 |
| CviAII | CATG | 4 cut(s) | 148, 247, 380, 578 |
| CviJI | RGCY | 8 cut(s) | 120, 132, 218, 224, 239, 284, 364, 507 |
| CviKI_1 | RGCY | 8 cut(s) | 120, 132, 218, 224, 239, 284, 364, 507 |
| CviQI | GTAC | 3 cut(s) | 151, 371, 383 |
| DdeI | CTNAG | 1 cut(s) | 501 |
| DpnI | GATC | 4 cut(s) | 53, 138, 171, 565 |
| DpnII | GATC | 4 cut(s) | 51, 136, 169, 563 |
| DraIII | CACNNNGTG | 1 cut(s) | 305 |
| EaeI | YGGCCR | 2 cut(s) | 237, 282 |
| EciI | GGCGGA | 3 cut(s) | 182, 470, 568 |
| Eco47I | GGWCC | 2 cut(s) | 19, 416 |
| Eco91I | GGTNACC | 1 cut(s) | 596 |
| EcoO109I | RGGNCCY | 1 cut(s) | 19 |
| EcoO65I | GGTNACC | 1 cut(s) | 596 |
| EcoRII | CCWGG | 5 cut(s) | 109, 323, 358, 397, 488 |
| EcoT22I | ATGCAT | 1 cut(s) | 381 |
| FaeI | CATG | 4 cut(s) | 151, 250, 383, 581 |
| FaiI | YATR | 8 cut(s) | 149, 177, 248, 381, 428, 521, 550, 579 |
| FaqI | GGGAC | 2 cut(s) | 32, 429 |
| FatI | CATG | 4 cut(s) | 147, 246, 379, 577 |
| FauI | CCCGC | 3 cut(s) | 8, 31, 55 |
| FblI | GTMKAC | 1 cut(s) | 334 |
| Fnu4HI | GCNGC | 6 cut(s) | 115, 118, 121, 240, 282, 319 |
| Fsp4HI | GCNGC | 6 cut(s) | 115, 118, 121, 240, 282, 319 |
| GlaI | GCGC | 3 cut(s) | 67, 206, 243 |
| GluI | GCNGC | 6 cut(s) | 115, 118, 121, 240, 282, 319 |
| GsaI | CCCAGC | 1 cut(s) | 342 |
| HaeIII | GGCC | 5 cut(s) | 132, 218, 239, 284, 364 |
| HapII | CCGG | 3 cut(s) | 93, 296, 365 |
| HhaI | GCGC | 3 cut(s) | 68, 207, 244 |
| Hin1II | CATG | 4 cut(s) | 151, 250, 383, 581 |
| Hin6I | GCGC | 3 cut(s) | 66, 205, 242 |
| HinP1I | GCGC | 3 cut(s) | 66, 205, 242 |
| HincII | GTYRAC | 2 cut(s) | 61, 335 |
| HindII | GTYRAC | 2 cut(s) | 61, 335 |
| HinfI | GANTC | 1 cut(s) | 458 |
| HpaI | GTTAAC | 1 cut(s) | 61 |
| HpaII | CCGG | 3 cut(s) | 93, 296, 365 |
| HphI | GGTGA | 3 cut(s) | 110, 136, 590 |
| Hpy166II | GTNNAC | 3 cut(s) | 61, 335, 575 |
| Hpy188I | TCNGA | 2 cut(s) | 43, 513 |
| Hpy188III | TCNNGA | 3 cut(s) | 247, 408, 590 |
| Hpy8I | GTNNAC | 3 cut(s) | 61, 335, 575 |
| HpyAV | CCTTC | 3 cut(s) | 208, 587, 596 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4V | TGCA | 1 cut(s) | 379 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 63, 120, 129, 385 |
| HpyF3I | CTNAG | 1 cut(s) | 501 |
| Hsp92II | CATG | 4 cut(s) | 151, 250, 383, 581 |
| HspAI | GCGC | 3 cut(s) | 66, 205, 242 |
| KflI | GGGWCCC | 1 cut(s) | 19 |
| KspAI | GTTAAC | 1 cut(s) | 61 |
| Kzo9I | GATC | 4 cut(s) | 51, 136, 169, 563 |
| LmnI | GCTCC | 1 cut(s) | 202 |
| Lsp1109I | GCAGC | 3 cut(s) | 126, 129, 330 |
| LweI | GCATC | 1 cut(s) | 345 |
| MaeIII | GTNAC | 3 cut(s) | 142, 312, 596 |
| MalI | GATC | 4 cut(s) | 53, 138, 171, 565 |
| MboI | GATC | 4 cut(s) | 51, 136, 169, 563 |
| MboII | GAAGA | 1 cut(s) | 164 |
| MflI | RGATCY | 2 cut(s) | 136, 169 |
| MmeI | TCCRAC | 1 cut(s) | 66 |
| MnlI | CCTC | 6 cut(s) | 49, 94, 190, 193, 253, 496 |
| Mph1103I | ATGCAT | 1 cut(s) | 381 |
| MroXI | GAANNNNTTC | 1 cut(s) | 462 |
| MseI | TTAA | 1 cut(s) | 60 |
| MslI | CAYNNNNRTG | 1 cut(s) | 353 |
| MspA1I | CMGCKG | 1 cut(s) | 388 |
| MspI | CCGG | 3 cut(s) | 93, 296, 365 |
| MspR9I | CCNGG | 7 cut(s) | 93, 111, 296, 325, 360, 399, 490 |
| Mva1269I | GAATGC | 1 cut(s) | 490 |
| MvaI | CCWGG | 5 cut(s) | 111, 325, 360, 399, 490 |
| MvnI | CGCG | 3 cut(s) | 50, 66, 242 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 63, 120, 129, 385 |
| NciI | CCSGG | 2 cut(s) | 93, 296 |
| NdeII | GATC | 4 cut(s) | 51, 136, 169, 563 |
| NlaIII | CATG | 4 cut(s) | 151, 250, 383, 581 |
| NlaIV | GGNNCC | 3 cut(s) | 20, 21, 417 |
| NmuCI | GTSAC | 3 cut(s) | 142, 312, 596 |
| NsiI | ATGCAT | 1 cut(s) | 381 |
| NspI | RCATGY | 1 cut(s) | 383 |
| PagI | TCATGA | 1 cut(s) | 246 |
| PctI | GAATGC | 1 cut(s) | 490 |
| PdmI | GAANNNNTTC | 1 cut(s) | 462 |
| PfeI | GAWTC | 1 cut(s) | 458 |
| PkrI | GCNGC | 6 cut(s) | 116, 119, 122, 241, 283, 320 |
| Ple19I | CGATCG | 1 cut(s) | 54 |
| PpuMI | RGGWCCY | 1 cut(s) | 19 |
| Psp5II | RGGWCCY | 1 cut(s) | 19 |
| Psp6I | CCWGG | 5 cut(s) | 109, 323, 358, 397, 488 |
| PspEI | GGTNACC | 1 cut(s) | 596 |
| PspFI | CCCAGC | 1 cut(s) | 338 |
| PspGI | CCWGG | 5 cut(s) | 109, 323, 358, 397, 488 |
| PspN4I | GGNNCC | 3 cut(s) | 20, 21, 417 |
| PspPI | GGNCC | 3 cut(s) | 19, 362, 416 |
| PspPPI | RGGWCCY | 1 cut(s) | 19 |
| PsuI | RGATCY | 2 cut(s) | 136, 169 |
| PvuI | CGATCG | 1 cut(s) | 54 |
| RsaI | GTAC | 3 cut(s) | 152, 372, 384 |
| RsaNI | GTAC | 3 cut(s) | 151, 371, 383 |
| RseI | CAYNNNNRTG | 1 cut(s) | 353 |
| SalI | GTCGAC | 1 cut(s) | 333 |
| SaqAI | TTAA | 1 cut(s) | 60 |
| SatI | GCNGC | 6 cut(s) | 115, 118, 121, 240, 282, 319 |
| Sau3AI | GATC | 4 cut(s) | 51, 136, 169, 563 |
| Sau96I | GGNCC | 3 cut(s) | 19, 362, 416 |
| ScrFI | CCNGG | 7 cut(s) | 93, 111, 296, 325, 360, 399, 490 |
| SetI | ASST | 4 cut(s) | 400, 453, 588, 598 |
| SfaNI | GCATC | 1 cut(s) | 345 |
| SinI | GGWCC | 2 cut(s) | 19, 416 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 353 |
| StyD4I | CCNGG | 7 cut(s) | 91, 109, 294, 323, 358, 397, 488 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 334 |
| TaqII | GACCGA | 1 cut(s) | 576 |
| TauI | GCSGC | 3 cut(s) | 123, 242, 284 |
| TfiI | GAWTC | 1 cut(s) | 458 |
| Tru1I | TTAA | 1 cut(s) | 60 |
| Tru9I | TTAA | 1 cut(s) | 60 |
| TscAI | CASTG | 1 cut(s) | 168 |
| TseFI | GTSAC | 3 cut(s) | 142, 312, 596 |
| TseI | GCWGC | 3 cut(s) | 114, 117, 318 |
| Tsp45I | GTSAC | 3 cut(s) | 142, 312, 596 |
| TspDTI | ATGAA | 1 cut(s) | 164 |
| TspRI | CASTG | 1 cut(s) | 168 |
| VpaK11BI | GGWCC | 2 cut(s) | 19, 416 |
| XceI | RCATGY | 1 cut(s) | 383 |
| XmiI | GTMKAC | 1 cut(s) | 334 |
| XmnI | GAANNNNTTC | 1 cut(s) | 462 |
| Zsp2I | ATGCAT | 1 cut(s) | 381 |