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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 295 |
| AccII | CGCG | 2 cut(s) | 267, 388 |
| AciI | CCGC | 6 cut(s) | 123, 170, 186, 216, 239, 388 |
| AclI | AACGTT | 1 cut(s) | 66 |
| AcsI | RAATTY | 1 cut(s) | 326 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AdeI | CACNNNGTG | 1 cut(s) | 399 |
| AfaI | GTAC | 2 cut(s) | 204, 429 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 124, 199, 250, 304, 416 |
| AgsI | TTSAA | 1 cut(s) | 463 |
| AjnI | CCWGG | 1 cut(s) | 198 |
| AluBI | AGCT | 2 cut(s) | 229, 475 |
| AluI | AGCT | 2 cut(s) | 229, 475 |
| Alw21I | GWGCWC | 2 cut(s) | 97, 395 |
| Alw44I | GTGCAC | 1 cut(s) | 93 |
| AoxI | GGCC | 2 cut(s) | 288, 294 |
| ApaLI | GTGCAC | 1 cut(s) | 93 |
| ApoI | RAATTY | 1 cut(s) | 326 |
| AspS9I | GGNCC | 2 cut(s) | 241, 295 |
| AvaII | GGWCC | 1 cut(s) | 241 |
| BaeGI | GKGCMC | 1 cut(s) | 97 |
| Bbv12I | GWGCWC | 2 cut(s) | 97, 395 |
| BciT130I | CCWGG | 1 cut(s) | 200 |
| BclI | TGATCA | 1 cut(s) | 40 |
| BfaI | CTAG | 3 cut(s) | 236, 396, 470 |
| BfmI | CTRYAG | 1 cut(s) | 165 |
| BfuAI | ACCTGC | 1 cut(s) | 295 |
| BisI | GCNGC | 2 cut(s) | 187, 216 |
| BlpI | GCTNAGC | 1 cut(s) | 177 |
| BlsI | GCNGC | 2 cut(s) | 188, 217 |
| Bme1390I | CCNGG | 1 cut(s) | 200 |
| Bme18I | GGWCC | 1 cut(s) | 241 |
| BmgT120I | GGNCC | 2 cut(s) | 241, 295 |
| BmrFI | CCNGG | 1 cut(s) | 200 |
| BmsI | GCATC | 1 cut(s) | 438 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 177 |
| BsaJI | CCNNGG | 3 cut(s) | 57, 198, 291 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 124, 199, 250, 304, 416 |
| Bse1I | ACTGG | 3 cut(s) | 74, 138, 421 |
| BseBI | CCWGG | 1 cut(s) | 200 |
| BseDI | CCNNGG | 3 cut(s) | 57, 198, 291 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 124, 199, 250, 304, 416 |
| BseMII | CTCAG | 1 cut(s) | 191 |
| BseNI | ACTGG | 3 cut(s) | 74, 138, 421 |
| BseSI | GKGCMC | 1 cut(s) | 97 |
| BseYI | CCCAGC | 1 cut(s) | 119 |
| BsgI | GTGCAG | 1 cut(s) | 421 |
| Bsh1236I | CGCG | 2 cut(s) | 267, 388 |
| BshFI | GGCC | 2 cut(s) | 290, 296 |
| BsiHKAI | GWGCWC | 2 cut(s) | 97, 395 |
| BslFI | GGGAC | 1 cut(s) | 501 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 124, 199, 250, 304, 416 |
| BsmFI | GGGAC | 1 cut(s) | 501 |
| BsnI | GGCC | 2 cut(s) | 290, 296 |
| Bsp1286I | GDGCHC | 2 cut(s) | 97, 395 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 177 |
| BspACI | CCGC | 6 cut(s) | 123, 170, 186, 216, 239, 388 |
| BspANI | GGCC | 2 cut(s) | 290, 296 |
| BspCNI | CTCAG | 1 cut(s) | 190 |
| BspFNI | CGCG | 2 cut(s) | 267, 388 |
| BspMI | ACCTGC | 1 cut(s) | 295 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrI | ACTGG | 3 cut(s) | 74, 138, 421 |
| BssECI | CCNNGG | 3 cut(s) | 57, 198, 291 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 1 cut(s) | 200 |
| Bst4CI | ACNGT | 4 cut(s) | 11, 61, 92, 370 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 348 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 399 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 231, 269, 380 |
| BstDEI | CTNAG | 1 cut(s) | 177 |
| BstDSI | CCRYGG | 1 cut(s) | 57 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 248 |
| BstFNI | CGCG | 2 cut(s) | 267, 388 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 129, 379, 399 |
| BstNI | CCWGG | 1 cut(s) | 200 |
| BstSCI | CCNGG | 1 cut(s) | 198 |
| BstSFI | CTRYAG | 1 cut(s) | 165 |
| BstSLI | GKGCMC | 1 cut(s) | 97 |
| BstUI | CGCG | 2 cut(s) | 267, 388 |
| BsuRI | GGCC | 2 cut(s) | 290, 296 |
| BtgI | CCRYGG | 1 cut(s) | 57 |
| BtsI | GCAGTG | 1 cut(s) | 286 |
| BtsIMutI | CAGTG | 4 cut(s) | 81, 286, 375, 414 |
| BveI | ACCTGC | 1 cut(s) | 295 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 231, 269, 380 |
| Cfr13I | GGNCC | 2 cut(s) | 241, 295 |
| Csp6I | GTAC | 2 cut(s) | 203, 428 |
| CviAII | CATG | 1 cut(s) | 272 |
| CviJI | RGCY | 9 cut(s) | 38, 176, 181, 229, 290, 296, 311, 409, 475 |
| CviKI_1 | RGCY | 9 cut(s) | 38, 176, 181, 229, 290, 296, 311, 409, 475 |
| CviQI | GTAC | 2 cut(s) | 203, 428 |
| DdeI | CTNAG | 1 cut(s) | 177 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| DraIII | CACNNNGTG | 1 cut(s) | 399 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 348 |
| EarI | CTCTTC | 2 cut(s) | 24, 348 |
| Eco47I | GGWCC | 1 cut(s) | 241 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 248 |
| EcoRII | CCWGG | 1 cut(s) | 198 |
| FaeI | CATG | 1 cut(s) | 275 |
| FaiI | YATR | 3 cut(s) | 221, 225, 273 |
| FaqI | GGGAC | 1 cut(s) | 501 |
| FatI | CATG | 1 cut(s) | 271 |
| FauI | CCCGC | 1 cut(s) | 116 |
| FbaI | TGATCA | 1 cut(s) | 40 |
| Fnu4HI | GCNGC | 2 cut(s) | 187, 216 |
| Fsp4HI | GCNGC | 2 cut(s) | 187, 216 |
| FspBI | CTAG | 3 cut(s) | 236, 396, 470 |
| GluI | GCNGC | 2 cut(s) | 187, 216 |
| GsaI | CCCAGC | 1 cut(s) | 123 |
| HaeIII | GGCC | 2 cut(s) | 290, 296 |
| Hin1II | CATG | 1 cut(s) | 275 |
| HincII | GTYRAC | 2 cut(s) | 64, 115 |
| HindII | GTYRAC | 2 cut(s) | 64, 115 |
| HinfI | GANTC | 1 cut(s) | 463 |
| Hpy166II | GTNNAC | 5 cut(s) | 64, 88, 95, 115, 430 |
| Hpy188III | TCNNGA | 1 cut(s) | 496 |
| Hpy8I | GTNNAC | 5 cut(s) | 64, 88, 95, 115, 430 |
| HpyAV | CCTTC | 2 cut(s) | 254, 337 |
| HpyCH4III | ACNGT | 4 cut(s) | 11, 61, 92, 370 |
| HpyCH4IV | ACGT | 1 cut(s) | 66 |
| HpyCH4V | TGCA | 5 cut(s) | 95, 373, 402, 445, 451 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 129, 379, 399 |
| HpyF3I | CTNAG | 1 cut(s) | 177 |
| HpySE526I | ACGT | 1 cut(s) | 66 |
| Hsp92II | CATG | 1 cut(s) | 275 |
| Ksp22I | TGATCA | 1 cut(s) | 40 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 390 |
| LweI | GCATC | 1 cut(s) | 438 |
| MaeI | CTAG | 3 cut(s) | 236, 396, 470 |
| MaeII | ACGT | 1 cut(s) | 66 |
| MaeIII | GTNAC | 1 cut(s) | 140 |
| MalI | GATC | 1 cut(s) | 42 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 2 cut(s) | 41, 365 |
| MhlI | GDGCHC | 2 cut(s) | 97, 395 |
| MluCI | AATT | 1 cut(s) | 326 |
| MmeI | TCCRAC | 1 cut(s) | 330 |
| MnlI | CCTC | 3 cut(s) | 166, 286, 503 |
| MseI | TTAA | 2 cut(s) | 69, 81 |
| MslI | CAYNNNNRTG | 1 cut(s) | 317 |
| MspA1I | CMGCKG | 2 cut(s) | 123, 170 |
| MspR9I | CCNGG | 1 cut(s) | 200 |
| MvaI | CCWGG | 1 cut(s) | 200 |
| MvnI | CGCG | 2 cut(s) | 267, 388 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 129, 379, 399 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIII | CATG | 1 cut(s) | 275 |
| NmeAIII | GCCGAG | 1 cut(s) | 316 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 463 |
| PkrI | GCNGC | 2 cut(s) | 188, 217 |
| Psp1406I | AACGTT | 1 cut(s) | 66 |
| Psp6I | CCWGG | 1 cut(s) | 198 |
| PspFI | CCCAGC | 1 cut(s) | 119 |
| PspGI | CCWGG | 1 cut(s) | 198 |
| PspPI | GGNCC | 2 cut(s) | 241, 295 |
| RsaI | GTAC | 2 cut(s) | 204, 429 |
| RsaNI | GTAC | 2 cut(s) | 203, 428 |
| RseI | CAYNNNNRTG | 1 cut(s) | 317 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 2 cut(s) | 69, 81 |
| SatI | GCNGC | 2 cut(s) | 187, 216 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 2 cut(s) | 241, 295 |
| ScrFI | CCNGG | 1 cut(s) | 200 |
| SduI | GDGCHC | 2 cut(s) | 97, 395 |
| SetI | ASST | 9 cut(s) | 69, 114, 147, 231, 246, 309, 401, 477, 495 |
| SfaNI | GCATC | 1 cut(s) | 438 |
| SfcI | CTRYAG | 1 cut(s) | 165 |
| SinI | GGWCC | 1 cut(s) | 241 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 317 |
| Sse9I | AATT | 1 cut(s) | 326 |
| SsiI | CCGC | 6 cut(s) | 123, 170, 186, 216, 239, 388 |
| SspMI | CTAG | 3 cut(s) | 236, 396, 470 |
| StyD4I | CCNGG | 1 cut(s) | 198 |
| TaaI | ACNGT | 4 cut(s) | 11, 61, 92, 370 |
| TaiI | ACGT | 1 cut(s) | 69 |
| TasI | AATT | 1 cut(s) | 326 |
| TauI | GCSGC | 2 cut(s) | 189, 218 |
| TfiI | GAWTC | 1 cut(s) | 463 |
| Tru1I | TTAA | 2 cut(s) | 69, 81 |
| Tru9I | TTAA | 2 cut(s) | 69, 81 |
| TscAI | CASTG | 4 cut(s) | 81, 286, 375, 421 |
| TspDTI | ATGAA | 2 cut(s) | 288, 348 |
| TspRI | CASTG | 4 cut(s) | 81, 286, 375, 421 |
| VneI | GTGCAC | 1 cut(s) | 93 |
| VpaK11BI | GGWCC | 1 cut(s) | 241 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 248 |
| XapI | RAATTY | 1 cut(s) | 326 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 421 |
| XspI | CTAG | 3 cut(s) | 236, 396, 470 |