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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 179 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 480 |
| AccB1I | GGYRCC | 2 cut(s) | 18, 987 |
| AccI | GTMKAC | 1 cut(s) | 472 |
| AccII | CGCG | 2 cut(s) | 677, 918 |
| AciI | CCGC | 8 cut(s) | 47, 497, 677, 893, 916, 918, 1042, 1105 |
| AclWI | GGATC | 4 cut(s) | 249, 614, 1043, 1056 |
| AcoI | YGGCCR | 3 cut(s) | 498, 702, 759 |
| AcyI | GRCGYC | 3 cut(s) | 19, 469, 988 |
| AfaI | GTAC | 1 cut(s) | 844 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 212, 711, 866, 980 |
| AgeI | ACCGGT | 1 cut(s) | 661 |
| AgsI | TTSAA | 3 cut(s) | 259, 542, 1005 |
| AjnI | CCWGG | 6 cut(s) | 205, 306, 396, 640, 666, 1044 |
| AluBI | AGCT | 7 cut(s) | 9, 116, 271, 284, 301, 746, 787 |
| AluI | AGCT | 7 cut(s) | 9, 116, 271, 284, 301, 746, 787 |
| Alw21I | GWGCWC | 2 cut(s) | 11, 118 |
| Alw26I | GTCTC | 2 cut(s) | 123, 600 |
| AlwI | GGATC | 4 cut(s) | 249, 614, 1043, 1056 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 654 |
| Ama87I | CYCGRG | 2 cut(s) | 315, 563 |
| AoxI | GGCC | 9 cut(s) | 252, 498, 702, 759, 909, 1026, 1032, 1057, 1106 |
| ApaI | GGGCCC | 1 cut(s) | 913 |
| ApeKI | GCWGC | 1 cut(s) | 950 |
| AsiGI | ACCGGT | 1 cut(s) | 661 |
| AspLEI | GCGC | 6 cut(s) | 21, 603, 799, 823, 990, 998 |
| AspS9I | GGNCC | 6 cut(s) | 319, 664, 670, 857, 909, 910 |
| AsuC2I | CCSGG | 1 cut(s) | 975 |
| AsuHPI | GGTGA | 3 cut(s) | 295, 413, 1031 |
| AvaI | CYCGRG | 2 cut(s) | 315, 563 |
| AvaII | GGWCC | 4 cut(s) | 319, 664, 670, 857 |
| BaeGI | GKGCMC | 1 cut(s) | 913 |
| BalI | TGGCCA | 1 cut(s) | 761 |
| BamHI | GGATCC | 1 cut(s) | 1048 |
| BanI | GGYRCC | 2 cut(s) | 18, 987 |
| BanII | GRGCYC | 3 cut(s) | 11, 118, 913 |
| Bbv12I | GWGCWC | 2 cut(s) | 11, 118 |
| BbvI | GCAGC | 1 cut(s) | 962 |
| BccI | CCATC | 2 cut(s) | 205, 445 |
| BceAI | ACGGC | 2 cut(s) | 717, 1044 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 224, 258 |
| BciT130I | CCWGG | 6 cut(s) | 207, 308, 398, 642, 668, 1046 |
| BclI | TGATCA | 1 cut(s) | 73 |
| BcnI | CCSGG | 1 cut(s) | 975 |
| BcoDI | GTCTC | 2 cut(s) | 123, 600 |
| BfaI | CTAG | 3 cut(s) | 285, 818, 902 |
| BfmI | CTRYAG | 2 cut(s) | 624, 1053 |
| BfoI | RGCGCY | 4 cut(s) | 22, 800, 991, 999 |
| BisI | GCNGC | 4 cut(s) | 498, 678, 951, 1106 |
| BlpI | GCTNAGC | 1 cut(s) | 889 |
| BlsI | GCNGC | 4 cut(s) | 499, 679, 952, 1107 |
| Bme1390I | CCNGG | 7 cut(s) | 207, 308, 398, 642, 668, 975, 1046 |
| Bme18I | GGWCC | 4 cut(s) | 319, 664, 670, 857 |
| BmeT110I | CYCGRG | 2 cut(s) | 315, 563 |
| BmgT120I | GGNCC | 6 cut(s) | 319, 664, 670, 857, 909, 910 |
| BmiI | GGNNCC | 6 cut(s) | 20, 320, 859, 911, 989, 1050 |
| BmrFI | CCNGG | 7 cut(s) | 207, 308, 398, 642, 668, 975, 1046 |
| BmsI | GCATC | 1 cut(s) | 139 |
| BpmI | CTGGAG | 3 cut(s) | 329, 821, 900 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 889 |
| BpuEI | CTTGAG | 2 cut(s) | 129, 535 |
| BpuMI | CCSGG | 1 cut(s) | 975 |
| BsaHI | GRCGYC | 3 cut(s) | 19, 469, 988 |
| BsaJI | CCNNGG | 6 cut(s) | 206, 306, 397, 564, 916, 1029 |
| BsaWI | WCCGGW | 1 cut(s) | 661 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 567, 597 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 212, 711, 866, 980 |
| Bse118I | RCCGGY | 1 cut(s) | 661 |
| Bse1I | ACTGG | 3 cut(s) | 220, 228, 476 |
| BseBI | CCWGG | 6 cut(s) | 207, 308, 398, 642, 668, 1046 |
| BseDI | CCNNGG | 6 cut(s) | 206, 306, 397, 564, 916, 1029 |
| BseGI | GGATG | 1 cut(s) | 216 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 212, 711, 866, 980 |
| BseMII | CTCAG | 2 cut(s) | 611, 903 |
| BseNI | ACTGG | 3 cut(s) | 220, 228, 476 |
| BseSI | GKGCMC | 1 cut(s) | 913 |
| BseX3I | CGGCCG | 1 cut(s) | 498 |
| BseXI | GCAGC | 1 cut(s) | 962 |
| BseYI | CCCAGC | 1 cut(s) | 992 |
| Bsh1236I | CGCG | 2 cut(s) | 677, 918 |
| Bsh1285I | CGRYCG | 1 cut(s) | 501 |
| BshFI | GGCC | 9 cut(s) | 254, 500, 704, 761, 911, 1028, 1034, 1059, 1108 |
| BshNI | GGYRCC | 2 cut(s) | 18, 987 |
| BshTI | ACCGGT | 1 cut(s) | 661 |
| BsiEI | CGRYCG | 1 cut(s) | 501 |
| BsiHKAI | GWGCWC | 2 cut(s) | 11, 118 |
| BsiHKCI | CYCGRG | 2 cut(s) | 315, 563 |
| BsiSI | CCGG | 3 cut(s) | 662, 867, 974 |
| BslFI | GGGAC | 1 cut(s) | 332 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 212, 711, 866, 980 |
| BsmAI | GTCTC | 2 cut(s) | 123, 600 |
| BsmFI | GGGAC | 1 cut(s) | 332 |
| BsmI | GAATGC | 2 cut(s) | 384, 742 |
| BsnI | GGCC | 9 cut(s) | 254, 500, 704, 761, 911, 1028, 1034, 1059, 1108 |
| BsoBI | CYCGRG | 2 cut(s) | 315, 563 |
| Bsp120I | GGGCCC | 1 cut(s) | 909 |
| Bsp1286I | GDGCHC | 3 cut(s) | 11, 118, 913 |
| Bsp143I | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 889 |
| BspACI | CCGC | 8 cut(s) | 47, 497, 677, 893, 916, 918, 1042, 1105 |
| BspANI | GGCC | 9 cut(s) | 254, 500, 704, 761, 911, 1028, 1034, 1059, 1108 |
| BspCNI | CTCAG | 2 cut(s) | 610, 902 |
| BspFNI | CGCG | 2 cut(s) | 677, 918 |
| BspHI | TCATGA | 1 cut(s) | 354 |
| BspLI | GGNNCC | 6 cut(s) | 20, 320, 859, 911, 989, 1050 |
| BspPI | GGATC | 4 cut(s) | 249, 614, 1043, 1056 |
| BspT107I | GGYRCC | 2 cut(s) | 18, 987 |
| BsrFI | RCCGGY | 1 cut(s) | 661 |
| BsrI | ACTGG | 3 cut(s) | 220, 228, 476 |
| BssAI | RCCGGY | 1 cut(s) | 661 |
| BssECI | CCNNGG | 6 cut(s) | 206, 306, 397, 564, 916, 1029 |
| BssMI | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| BssNI | GRCGYC | 3 cut(s) | 19, 469, 988 |
| BssT1I | CCWWGG | 1 cut(s) | 1029 |
| Bst2UI | CCWGG | 6 cut(s) | 207, 308, 398, 642, 668, 1046 |
| Bst4CI | ACNGT | 2 cut(s) | 37, 628 |
| BstACI | GRCGYC | 3 cut(s) | 19, 469, 988 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 654 |
| BstC8I | GCNNGC | 3 cut(s) | 51, 364, 830 |
| BstDEI | CTNAG | 5 cut(s) | 389, 445, 597, 889, 938 |
| BstDSI | CCRYGG | 1 cut(s) | 916 |
| BstF5I | GGATG | 1 cut(s) | 216 |
| BstFNI | CGCG | 2 cut(s) | 677, 918 |
| BstH2I | RGCGCY | 4 cut(s) | 22, 800, 991, 999 |
| BstHHI | GCGC | 6 cut(s) | 21, 603, 799, 823, 990, 998 |
| BstKTI | GATC | 4 cut(s) | 76, 244, 609, 1051 |
| BstMAI | GTCTC | 2 cut(s) | 123, 600 |
| BstMBI | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| BstMCI | CGRYCG | 1 cut(s) | 501 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 654, 701, 803, 818, 829, 959, 1091 |
| BstNI | CCWGG | 6 cut(s) | 207, 308, 398, 642, 668, 1046 |
| BstNSI | RCATGY | 1 cut(s) | 1077 |
| BstSCI | CCNGG | 7 cut(s) | 205, 306, 396, 640, 666, 973, 1044 |
| BstSFI | CTRYAG | 2 cut(s) | 624, 1053 |
| BstSLI | GKGCMC | 1 cut(s) | 913 |
| BstUI | CGCG | 2 cut(s) | 677, 918 |
| BstV1I | GCAGC | 1 cut(s) | 962 |
| BstX2I | RGATCY | 2 cut(s) | 606, 1048 |
| BstYI | RGATCY | 2 cut(s) | 606, 1048 |
| BstZI | CGGCCG | 1 cut(s) | 498 |
| BsuRI | GGCC | 9 cut(s) | 254, 500, 704, 761, 911, 1028, 1034, 1059, 1108 |
| BtgI | CCRYGG | 1 cut(s) | 916 |
| BtsCI | GGATG | 1 cut(s) | 216 |
| BtsI | GCAGTG | 3 cut(s) | 165, 242, 652 |
| BtsIMutI | CAGTG | 5 cut(s) | 165, 242, 633, 652, 695 |
| Cac8I | GCNNGC | 3 cut(s) | 51, 364, 830 |
| CaiI | CAGNNNCTG | 1 cut(s) | 654 |
| CciI | TCATGA | 1 cut(s) | 354 |
| CfoI | GCGC | 6 cut(s) | 21, 603, 799, 823, 990, 998 |
| Cfr10I | RCCGGY | 1 cut(s) | 661 |
| Cfr13I | GGNCC | 6 cut(s) | 319, 664, 670, 857, 909, 910 |
| Cfr42I | CCGCGG | 1 cut(s) | 919 |
| CseI | GACGC | 4 cut(s) | 422, 458, 956, 1009 |
| Csp6I | GTAC | 1 cut(s) | 843 |
| CspAI | ACCGGT | 1 cut(s) | 661 |
| CviAII | CATG | 7 cut(s) | 23, 155, 250, 355, 441, 763, 1074 |
| CviQI | GTAC | 1 cut(s) | 843 |
| DdeI | CTNAG | 5 cut(s) | 389, 445, 597, 889, 938 |
| DinI | GGCGCC | 2 cut(s) | 20, 989 |
| DpnI | GATC | 4 cut(s) | 75, 243, 608, 1050 |
| DpnII | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 480 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 480 |
| EaeI | YGGCCR | 3 cut(s) | 498, 702, 759 |
| EagI | CGGCCG | 1 cut(s) | 498 |
| EciI | GGCGGA | 1 cut(s) | 36 |
| Ecl136II | GAGCTC | 2 cut(s) | 9, 116 |
| EclXI | CGGCCG | 1 cut(s) | 498 |
| Eco130I | CCWWGG | 1 cut(s) | 1029 |
| Eco24I | GRGCYC | 3 cut(s) | 11, 118, 913 |
| Eco47I | GGWCC | 4 cut(s) | 319, 664, 670, 857 |
| Eco52I | CGGCCG | 1 cut(s) | 498 |
| Eco53kI | GAGCTC | 2 cut(s) | 9, 116 |
| Eco88I | CYCGRG | 2 cut(s) | 315, 563 |
| EcoICRI | GAGCTC | 2 cut(s) | 9, 116 |
| EcoO109I | RGGNCCY | 3 cut(s) | 319, 670, 909 |
| EcoRII | CCWGG | 6 cut(s) | 205, 306, 396, 640, 666, 1044 |
| EcoT14I | CCWWGG | 1 cut(s) | 1029 |
| EcoT38I | GRGCYC | 3 cut(s) | 11, 118, 913 |
| EgeI | GGCGCC | 2 cut(s) | 20, 989 |
| EheI | GGCGCC | 2 cut(s) | 20, 989 |
| ErhI | CCWWGG | 1 cut(s) | 1029 |
| FaeI | CATG | 7 cut(s) | 26, 158, 253, 358, 444, 766, 1077 |
| FaqI | GGGAC | 1 cut(s) | 332 |
| FatI | CATG | 7 cut(s) | 22, 154, 249, 354, 440, 762, 1073 |
| FbaI | TGATCA | 1 cut(s) | 73 |
| FblI | GTMKAC | 1 cut(s) | 472 |
| Fnu4HI | GCNGC | 4 cut(s) | 498, 678, 951, 1106 |
| FokI | GGATG | 1 cut(s) | 223 |
| FriOI | GRGCYC | 3 cut(s) | 11, 118, 913 |
| Fsp4HI | GCNGC | 4 cut(s) | 498, 678, 951, 1106 |
| FspBI | CTAG | 3 cut(s) | 285, 818, 902 |
| GlaI | GCGC | 6 cut(s) | 20, 602, 798, 822, 989, 997 |
| GluI | GCNGC | 4 cut(s) | 498, 678, 951, 1106 |
| GsaI | CCCAGC | 1 cut(s) | 996 |
| GsuI | CTGGAG | 3 cut(s) | 329, 821, 900 |
| HaeII | RGCGCY | 4 cut(s) | 22, 800, 991, 999 |
| HaeIII | GGCC | 9 cut(s) | 254, 500, 704, 761, 911, 1028, 1034, 1059, 1108 |
| HapII | CCGG | 3 cut(s) | 662, 867, 974 |
| HgaI | GACGC | 4 cut(s) | 422, 458, 956, 1009 |
| HhaI | GCGC | 6 cut(s) | 21, 603, 799, 823, 990, 998 |
| Hin1I | GRCGYC | 3 cut(s) | 19, 469, 988 |
| Hin1II | CATG | 7 cut(s) | 26, 158, 253, 358, 444, 766, 1077 |
| Hin6I | GCGC | 6 cut(s) | 19, 601, 797, 821, 988, 996 |
| HinP1I | GCGC | 6 cut(s) | 19, 601, 797, 821, 988, 996 |
| HincII | GTYRAC | 3 cut(s) | 122, 473, 532 |
| HindII | GTYRAC | 3 cut(s) | 122, 473, 532 |
| HindIII | AAGCTT | 1 cut(s) | 269 |
| HinfI | GANTC | 4 cut(s) | 142, 404, 593, 896 |
| HpaII | CCGG | 3 cut(s) | 662, 867, 974 |
| HphI | GGTGA | 3 cut(s) | 295, 413, 1031 |
| Hpy166II | GTNNAC | 5 cut(s) | 122, 225, 473, 532, 857 |
| Hpy188I | TCNGA | 2 cut(s) | 128, 415 |
| Hpy188III | TCNNGA | 5 cut(s) | 107, 146, 259, 317, 355 |
| Hpy8I | GTNNAC | 5 cut(s) | 122, 225, 473, 532, 857 |
| Hpy99I | CGWCG | 1 cut(s) | 474 |
| HpyAV | CCTTC | 4 cut(s) | 104, 265, 787, 1130 |
| HpyCH4III | ACNGT | 2 cut(s) | 37, 628 |
| HpyCH4IV | ACGT | 2 cut(s) | 850, 922 |
| HpyCH4V | TGCA | 5 cut(s) | 235, 348, 366, 384, 1077 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 654, 701, 803, 818, 829, 959, 1091 |
| HpyF3I | CTNAG | 5 cut(s) | 389, 445, 597, 889, 938 |
| HpySE526I | ACGT | 2 cut(s) | 850, 922 |
| Hsp92I | GRCGYC | 3 cut(s) | 19, 469, 988 |
| Hsp92II | CATG | 7 cut(s) | 26, 158, 253, 358, 444, 766, 1077 |
| HspAI | GCGC | 6 cut(s) | 19, 601, 797, 821, 988, 996 |
| KasI | GGCGCC | 2 cut(s) | 18, 987 |
| Ksp22I | TGATCA | 1 cut(s) | 73 |
| KspI | CCGCGG | 1 cut(s) | 919 |
| Kzo9I | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| LmnI | GCTCC | 2 cut(s) | 113, 959 |
| Lsp1109I | GCAGC | 1 cut(s) | 962 |
| LweI | GCATC | 1 cut(s) | 139 |
| MaeI | CTAG | 3 cut(s) | 285, 818, 902 |
| MaeII | ACGT | 2 cut(s) | 850, 922 |
| MaeIII | GTNAC | 4 cut(s) | 171, 419, 650, 1148 |
| MalI | GATC | 4 cut(s) | 75, 243, 608, 1050 |
| MboI | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| MfeI | CAATTG | 1 cut(s) | 954 |
| MflI | RGATCY | 2 cut(s) | 606, 1048 |
| MhlI | GDGCHC | 3 cut(s) | 11, 118, 913 |
| MlsI | TGGCCA | 1 cut(s) | 761 |
| MluCI | AATT | 4 cut(s) | 450, 925, 954, 1000 |
| MluNI | TGGCCA | 1 cut(s) | 761 |
| Mly113I | GGCGCC | 2 cut(s) | 19, 988 |
| MlyI | GAGTC | 2 cut(s) | 136, 602 |
| Mox20I | TGGCCA | 1 cut(s) | 761 |
| MscI | TGGCCA | 1 cut(s) | 761 |
| MseI | TTAA | 1 cut(s) | 56 |
| MslI | CAYNNNNRTG | 2 cut(s) | 353, 767 |
| Msp20I | TGGCCA | 1 cut(s) | 761 |
| MspA1I | CMGCKG | 3 cut(s) | 893, 918, 1105 |
| MspI | CCGG | 3 cut(s) | 662, 867, 974 |
| MspR9I | CCNGG | 7 cut(s) | 207, 308, 398, 642, 668, 975, 1046 |
| MunI | CAATTG | 1 cut(s) | 954 |
| Mva1269I | GAATGC | 2 cut(s) | 384, 742 |
| MvaI | CCWGG | 6 cut(s) | 207, 308, 398, 642, 668, 1046 |
| MvnI | CGCG | 2 cut(s) | 677, 918 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 654, 701, 803, 818, 829, 959, 1091 |
| NarI | GGCGCC | 2 cut(s) | 19, 988 |
| NciI | CCSGG | 1 cut(s) | 975 |
| NdeII | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| NlaIII | CATG | 7 cut(s) | 26, 158, 253, 358, 444, 766, 1077 |
| NlaIV | GGNNCC | 6 cut(s) | 20, 320, 859, 911, 989, 1050 |
| NmeAIII | GCCGAG | 1 cut(s) | 526 |
| NmuCI | GTSAC | 2 cut(s) | 650, 1148 |
| NspI | RCATGY | 1 cut(s) | 1077 |
| PagI | TCATGA | 1 cut(s) | 354 |
| PctI | GAATGC | 2 cut(s) | 384, 742 |
| PfeI | GAWTC | 2 cut(s) | 404, 896 |
| PfoI | TCCNGGA | 1 cut(s) | 666 |
| PinAI | ACCGGT | 1 cut(s) | 661 |
| PkrI | GCNGC | 4 cut(s) | 499, 679, 952, 1107 |
| PleI | GAGTC | 2 cut(s) | 136, 601 |
| PluTI | GGCGCC | 2 cut(s) | 22, 991 |
| PpsI | GAGTC | 2 cut(s) | 136, 601 |
| PpuMI | RGGWCCY | 2 cut(s) | 319, 670 |
| PsiI | TTATAA | 1 cut(s) | 179 |
| Psp124BI | GAGCTC | 2 cut(s) | 11, 118 |
| Psp5II | RGGWCCY | 2 cut(s) | 319, 670 |
| Psp6I | CCWGG | 6 cut(s) | 205, 306, 396, 640, 666, 1044 |
| PspFI | CCCAGC | 1 cut(s) | 992 |
| PspGI | CCWGG | 6 cut(s) | 205, 306, 396, 640, 666, 1044 |
| PspN4I | GGNNCC | 6 cut(s) | 20, 320, 859, 911, 989, 1050 |
| PspOMI | GGGCCC | 1 cut(s) | 909 |
| PspPI | GGNCC | 6 cut(s) | 319, 664, 670, 857, 909, 910 |
| PspPPI | RGGWCCY | 2 cut(s) | 319, 670 |
| PstNI | CAGNNNCTG | 1 cut(s) | 654 |
| PsuI | RGATCY | 2 cut(s) | 606, 1048 |
| RsaI | GTAC | 1 cut(s) | 844 |
| RsaNI | GTAC | 1 cut(s) | 843 |
| RseI | CAYNNNNRTG | 2 cut(s) | 353, 767 |
| SacI | GAGCTC | 2 cut(s) | 11, 118 |
| SacII | CCGCGG | 1 cut(s) | 919 |
| SalI | GTCGAC | 1 cut(s) | 471 |
| SaqAI | TTAA | 1 cut(s) | 56 |
| SatI | GCNGC | 4 cut(s) | 498, 678, 951, 1106 |
| Sau3AI | GATC | 4 cut(s) | 73, 241, 606, 1048 |
| Sau96I | GGNCC | 6 cut(s) | 319, 664, 670, 857, 909, 910 |
| SchI | GAGTC | 2 cut(s) | 136, 602 |
| ScrFI | CCNGG | 7 cut(s) | 207, 308, 398, 642, 668, 975, 1046 |
| SduI | GDGCHC | 3 cut(s) | 11, 118, 913 |
| SfaNI | GCATC | 1 cut(s) | 139 |
| SfcI | CTRYAG | 2 cut(s) | 624, 1053 |
| SfoI | GGCGCC | 2 cut(s) | 20, 989 |
| Sfr303I | CCGCGG | 1 cut(s) | 919 |
| SgrBI | CCGCGG | 1 cut(s) | 919 |
| SinI | GGWCC | 4 cut(s) | 319, 664, 670, 857 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 353, 767 |
| SmlI | CTYRAG | 2 cut(s) | 144, 514 |
| SmoI | CTYRAG | 2 cut(s) | 144, 514 |
| Sse9I | AATT | 4 cut(s) | 450, 925, 954, 1000 |
| SsiI | CCGC | 8 cut(s) | 47, 497, 677, 893, 916, 918, 1042, 1105 |
| SspDI | GGCGCC | 2 cut(s) | 18, 987 |
| SspI | AATATT | 1 cut(s) | 873 |
| SspMI | CTAG | 3 cut(s) | 285, 818, 902 |
| SstI | GAGCTC | 2 cut(s) | 11, 118 |
| StyD4I | CCNGG | 7 cut(s) | 205, 306, 396, 640, 666, 973, 1044 |
| StyI | CCWWGG | 1 cut(s) | 1029 |
| TaaI | ACNGT | 2 cut(s) | 37, 628 |
| TaiI | ACGT | 2 cut(s) | 853, 925 |
| TaqI | TCGA | 2 cut(s) | 374, 472 |
| TasI | AATT | 4 cut(s) | 450, 925, 954, 1000 |
| TauI | GCSGC | 3 cut(s) | 500, 680, 1108 |
| TfiI | GAWTC | 2 cut(s) | 404, 896 |
| Tru1I | TTAA | 1 cut(s) | 56 |
| Tru9I | TTAA | 1 cut(s) | 56 |
| TscAI | CASTG | 5 cut(s) | 172, 242, 633, 659, 702 |
| TseFI | GTSAC | 2 cut(s) | 650, 1148 |
| TseI | GCWGC | 1 cut(s) | 950 |
| Tsp45I | GTSAC | 2 cut(s) | 650, 1148 |
| TspDTI | ATGAA | 4 cut(s) | 39, 238, 294, 343 |
| TspGWI | ACGGA | 2 cut(s) | 74, 99 |
| TspRI | CASTG | 5 cut(s) | 172, 242, 633, 659, 702 |
| VpaK11BI | GGWCC | 4 cut(s) | 319, 664, 670, 857 |
| XceI | RCATGY | 1 cut(s) | 1077 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 483 |
| XmiI | GTMKAC | 1 cut(s) | 472 |
| XspI | CTAG | 3 cut(s) | 285, 818, 902 |