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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 205 |
| Acc36I | ACCTGC | 2 cut(s) | 205, 298 |
| AccB1I | GGYRCC | 1 cut(s) | 121 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 352 |
| AccBSI | CCGCTC | 1 cut(s) | 246 |
| AccI | GTMKAC | 1 cut(s) | 100 |
| AccII | CGCG | 2 cut(s) | 82, 461 |
| AclWI | GGATC | 3 cut(s) | 269, 379, 457 |
| AcoI | YGGCCR | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 122 |
| AfaI | GTAC | 2 cut(s) | 138, 150 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 110, 261, 288, 294, 352 |
| AflIII | ACRYGT | 1 cut(s) | 17 |
| AjnI | CCWGG | 5 cut(s) | 89, 124, 163, 254, 287 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 26, 58 |
| AluBI | AGCT | 1 cut(s) | 349 |
| AluI | AGCT | 1 cut(s) | 349 |
| Alw26I | GTCTC | 1 cut(s) | 389 |
| AlwI | GGATC | 3 cut(s) | 269, 379, 457 |
| AoxI | GGCC | 5 cut(s) | 48, 128, 296, 318, 445 |
| ApeKI | GCWGC | 2 cut(s) | 5, 84 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 228 |
| AspLEI | GCGC | 4 cut(s) | 10, 84, 124, 294 |
| AspS9I | GGNCC | 4 cut(s) | 128, 182, 264, 446 |
| AsuC2I | CCSGG | 3 cut(s) | 389, 407, 444 |
| AsuHPI | GGTGA | 1 cut(s) | 284 |
| AvaII | GGWCC | 2 cut(s) | 182, 264 |
| BanI | GGYRCC | 1 cut(s) | 121 |
| BbvI | GCAGC | 1 cut(s) | 96 |
| BciT130I | CCWGG | 5 cut(s) | 91, 126, 165, 256, 289 |
| BcnI | CCSGG | 3 cut(s) | 389, 407, 444 |
| BcoDI | GTCTC | 1 cut(s) | 389 |
| BfaI | CTAG | 1 cut(s) | 417 |
| BfmI | CTRYAG | 1 cut(s) | 467 |
| BfoI | RGCGCY | 1 cut(s) | 125 |
| BfuAI | ACCTGC | 2 cut(s) | 205, 298 |
| BisI | GCNGC | 4 cut(s) | 6, 48, 85, 459 |
| BlsI | GCNGC | 4 cut(s) | 7, 49, 86, 460 |
| Bme1390I | CCNGG | 8 cut(s) | 91, 126, 165, 256, 289, 389, 407, 444 |
| Bme18I | GGWCC | 2 cut(s) | 182, 264 |
| BmgT120I | GGNCC | 4 cut(s) | 128, 182, 264, 446 |
| BmiI | GGNNCC | 4 cut(s) | 123, 183, 422, 448 |
| BmrFI | CCNGG | 8 cut(s) | 91, 126, 165, 256, 289, 389, 407, 444 |
| BmsI | GCATC | 3 cut(s) | 325, 351, 419 |
| BoxI | GACNNNNGTC | 1 cut(s) | 401 |
| BpmI | CTGGAG | 1 cut(s) | 392 |
| BpuMI | CCSGG | 3 cut(s) | 389, 407, 444 |
| BsaHI | GRCGYC | 1 cut(s) | 122 |
| BsaJI | CCNNGG | 4 cut(s) | 125, 164, 255, 287 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 365, 395 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 110, 261, 288, 294, 352 |
| Bse118I | RCCGGY | 2 cut(s) | 110, 130 |
| Bse1I | ACTGG | 1 cut(s) | 375 |
| BseBI | CCWGG | 5 cut(s) | 91, 126, 165, 256, 289 |
| BseDI | CCNNGG | 4 cut(s) | 125, 164, 255, 287 |
| BseGI | GGATG | 1 cut(s) | 366 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 110, 261, 288, 294, 352 |
| BseMII | CTCAG | 1 cut(s) | 317 |
| BseNI | ACTGG | 1 cut(s) | 375 |
| BseXI | GCAGC | 1 cut(s) | 96 |
| BseYI | CCCAGC | 2 cut(s) | 61, 345 |
| Bsh1236I | CGCG | 2 cut(s) | 82, 461 |
| Bsh1285I | CGRYCG | 2 cut(s) | 58, 135 |
| BshFI | GGCC | 5 cut(s) | 50, 130, 298, 320, 447 |
| BshNI | GGYRCC | 1 cut(s) | 121 |
| BsiEI | CGRYCG | 2 cut(s) | 58, 135 |
| BsiSI | CCGG | 5 cut(s) | 111, 131, 388, 407, 444 |
| BslFI | GGGAC | 2 cut(s) | 195, 389 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 110, 261, 288, 294, 352 |
| BsmAI | GTCTC | 1 cut(s) | 389 |
| BsmFI | GGGAC | 2 cut(s) | 195, 389 |
| BsmI | GAATGC | 1 cut(s) | 256 |
| BsnI | GGCC | 5 cut(s) | 50, 130, 298, 320, 447 |
| Bsp143I | GATC | 3 cut(s) | 274, 384, 462 |
| BspANI | GGCC | 5 cut(s) | 50, 130, 298, 320, 447 |
| BspCNI | CTCAG | 1 cut(s) | 316 |
| BspFNI | CGCG | 2 cut(s) | 82, 461 |
| BspHI | TCATGA | 1 cut(s) | 12 |
| BspLI | GGNNCC | 4 cut(s) | 123, 183, 422, 448 |
| BspMAI | CTGCAG | 1 cut(s) | 471 |
| BspMI | ACCTGC | 2 cut(s) | 205, 298 |
| BspPI | GGATC | 3 cut(s) | 269, 379, 457 |
| BspT107I | GGYRCC | 1 cut(s) | 121 |
| BsrBI | CCGCTC | 1 cut(s) | 246 |
| BsrFI | RCCGGY | 2 cut(s) | 110, 130 |
| BsrI | ACTGG | 1 cut(s) | 375 |
| BssAI | RCCGGY | 2 cut(s) | 110, 130 |
| BssECI | CCNNGG | 4 cut(s) | 125, 164, 255, 287 |
| BssMI | GATC | 3 cut(s) | 274, 384, 462 |
| BssNI | GRCGYC | 1 cut(s) | 122 |
| Bst2UI | CCWGG | 5 cut(s) | 91, 126, 165, 256, 289 |
| BstACI | GRCGYC | 1 cut(s) | 122 |
| BstC8I | GCNNGC | 4 cut(s) | 74, 112, 296, 471 |
| BstDEI | CTNAG | 1 cut(s) | 303 |
| BstF5I | GGATG | 1 cut(s) | 366 |
| BstFNI | CGCG | 2 cut(s) | 82, 461 |
| BstH2I | RGCGCY | 1 cut(s) | 125 |
| BstHHI | GCGC | 4 cut(s) | 10, 84, 124, 294 |
| BstKTI | GATC | 3 cut(s) | 277, 387, 465 |
| BstMAI | GTCTC | 1 cut(s) | 389 |
| BstMBI | GATC | 3 cut(s) | 274, 384, 462 |
| BstMCI | CGRYCG | 2 cut(s) | 58, 135 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 151, 304, 326, 335 |
| BstNI | CCWGG | 5 cut(s) | 91, 126, 165, 256, 289 |
| BstNSI | RCATGY | 1 cut(s) | 149 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 401 |
| BstSCI | CCNGG | 8 cut(s) | 89, 124, 163, 254, 287, 387, 405, 442 |
| BstSFI | CTRYAG | 1 cut(s) | 467 |
| BstUI | CGCG | 2 cut(s) | 82, 461 |
| BstV1I | GCAGC | 1 cut(s) | 96 |
| BsuRI | GGCC | 5 cut(s) | 50, 130, 298, 320, 447 |
| BtsCI | GGATG | 1 cut(s) | 366 |
| BveI | ACCTGC | 2 cut(s) | 205, 298 |
| Cac8I | GCNNGC | 4 cut(s) | 74, 112, 296, 471 |
| CciI | TCATGA | 1 cut(s) | 12 |
| CfoI | GCGC | 4 cut(s) | 10, 84, 124, 294 |
| Cfr10I | RCCGGY | 2 cut(s) | 110, 130 |
| Cfr13I | GGNCC | 4 cut(s) | 128, 182, 264, 446 |
| Csp6I | GTAC | 2 cut(s) | 137, 149 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 150, 185 |
| CviAII | CATG | 2 cut(s) | 13, 146 |
| CviJI | RGCY | 9 cut(s) | 5, 50, 110, 130, 298, 320, 349, 447, 473 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 50, 110, 130, 298, 320, 349, 447, 473 |
| CviQI | GTAC | 2 cut(s) | 137, 149 |
| DdeI | CTNAG | 1 cut(s) | 303 |
| DinI | GGCGCC | 1 cut(s) | 123 |
| DpnI | GATC | 3 cut(s) | 276, 386, 464 |
| DpnII | GATC | 3 cut(s) | 274, 384, 462 |
| EaeI | YGGCCR | 1 cut(s) | 48 |
| EciI | GGCGGA | 4 cut(s) | 236, 267, 316, 407 |
| Eco47I | GGWCC | 2 cut(s) | 182, 264 |
| EcoRII | CCWGG | 5 cut(s) | 89, 124, 163, 254, 287 |
| EcoT22I | ATGCAT | 1 cut(s) | 147 |
| EgeI | GGCGCC | 1 cut(s) | 123 |
| EheI | GGCGCC | 1 cut(s) | 123 |
| FaeI | CATG | 2 cut(s) | 16, 149 |
| FaiI | YATR | 6 cut(s) | 14, 147, 194, 284, 487, 489 |
| FaqI | GGGAC | 2 cut(s) | 195, 389 |
| FatI | CATG | 2 cut(s) | 12, 145 |
| FauI | CCCGC | 1 cut(s) | 432 |
| FauNDI | CATATG | 1 cut(s) | 487 |
| FblI | GTMKAC | 1 cut(s) | 100 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 48, 85, 459 |
| FokI | GGATG | 1 cut(s) | 373 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 48, 85, 459 |
| FspBI | CTAG | 1 cut(s) | 417 |
| GlaI | GCGC | 4 cut(s) | 9, 83, 123, 293 |
| GluI | GCNGC | 4 cut(s) | 6, 48, 85, 459 |
| GsaI | CCCAGC | 2 cut(s) | 65, 349 |
| GsuI | CTGGAG | 1 cut(s) | 392 |
| HaeII | RGCGCY | 1 cut(s) | 125 |
| HaeIII | GGCC | 5 cut(s) | 50, 130, 298, 320, 447 |
| HapII | CCGG | 5 cut(s) | 111, 131, 388, 407, 444 |
| HhaI | GCGC | 4 cut(s) | 10, 84, 124, 294 |
| Hin1I | GRCGYC | 1 cut(s) | 122 |
| Hin1II | CATG | 2 cut(s) | 16, 149 |
| Hin6I | GCGC | 4 cut(s) | 8, 82, 122, 292 |
| HinP1I | GCGC | 4 cut(s) | 8, 82, 122, 292 |
| HincII | GTYRAC | 1 cut(s) | 101 |
| HindII | GTYRAC | 1 cut(s) | 101 |
| HinfI | GANTC | 2 cut(s) | 224, 323 |
| HpaII | CCGG | 5 cut(s) | 111, 131, 388, 407, 444 |
| HphI | GGTGA | 1 cut(s) | 284 |
| Hpy166II | GTNNAC | 1 cut(s) | 101 |
| Hpy188I | TCNGA | 1 cut(s) | 479 |
| Hpy188III | TCNNGA | 3 cut(s) | 13, 174, 438 |
| Hpy8I | GTNNAC | 1 cut(s) | 101 |
| Hpy99I | CGWCG | 1 cut(s) | 335 |
| HpyAV | CCTTC | 1 cut(s) | 263 |
| HpyCH4IV | ACGT | 1 cut(s) | 19 |
| HpyCH4V | TGCA | 4 cut(s) | 145, 338, 364, 469 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 151, 304, 326, 335 |
| HpyF3I | CTNAG | 1 cut(s) | 303 |
| HpySE526I | ACGT | 1 cut(s) | 19 |
| Hsp92I | GRCGYC | 1 cut(s) | 122 |
| Hsp92II | CATG | 2 cut(s) | 16, 149 |
| HspAI | GCGC | 4 cut(s) | 8, 82, 122, 292 |
| KasI | GGCGCC | 1 cut(s) | 121 |
| KroI | GCCGGC | 1 cut(s) | 110 |
| KroNI | GCCGGC | 1 cut(s) | 112 |
| Kzo9I | GATC | 3 cut(s) | 274, 384, 462 |
| LmnI | GCTCC | 1 cut(s) | 373 |
| Lsp1109I | GCAGC | 1 cut(s) | 96 |
| LweI | GCATC | 3 cut(s) | 325, 351, 419 |
| MaeI | CTAG | 1 cut(s) | 417 |
| MaeII | ACGT | 1 cut(s) | 19 |
| MalI | GATC | 3 cut(s) | 276, 386, 464 |
| MbiI | CCGCTC | 1 cut(s) | 246 |
| MboI | GATC | 3 cut(s) | 274, 384, 462 |
| Mly113I | GGCGCC | 1 cut(s) | 122 |
| MmeI | TCCRAC | 1 cut(s) | 264 |
| MnlI | CCTC | 3 cut(s) | 19, 207, 372 |
| Mph1103I | ATGCAT | 1 cut(s) | 147 |
| MroNI | GCCGGC | 1 cut(s) | 110 |
| MroXI | GAANNNNTTC | 1 cut(s) | 228 |
| MspA1I | CMGCKG | 1 cut(s) | 154 |
| MspI | CCGG | 5 cut(s) | 111, 131, 388, 407, 444 |
| MspR9I | CCNGG | 8 cut(s) | 91, 126, 165, 256, 289, 389, 407, 444 |
| Mva1269I | GAATGC | 1 cut(s) | 256 |
| MvaI | CCWGG | 5 cut(s) | 91, 126, 165, 256, 289 |
| MvnI | CGCG | 2 cut(s) | 82, 461 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 151, 304, 326, 335 |
| NaeI | GCCGGC | 1 cut(s) | 112 |
| NarI | GGCGCC | 1 cut(s) | 122 |
| NciI | CCSGG | 3 cut(s) | 389, 407, 444 |
| NdeI | CATATG | 1 cut(s) | 487 |
| NdeII | GATC | 3 cut(s) | 274, 384, 462 |
| NgoMIV | GCCGGC | 1 cut(s) | 110 |
| NlaIII | CATG | 2 cut(s) | 16, 149 |
| NlaIV | GGNNCC | 4 cut(s) | 123, 183, 422, 448 |
| NsiI | ATGCAT | 1 cut(s) | 147 |
| NspI | RCATGY | 1 cut(s) | 149 |
| PagI | TCATGA | 1 cut(s) | 12 |
| PaqCI | CACCTGC | 1 cut(s) | 205 |
| PctI | GAATGC | 1 cut(s) | 256 |
| PdiI | GCCGGC | 1 cut(s) | 112 |
| PdmI | GAANNNNTTC | 1 cut(s) | 228 |
| PfeI | GAWTC | 2 cut(s) | 224, 323 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 352 |
| PkrI | GCNGC | 4 cut(s) | 7, 49, 86, 460 |
| PluTI | GGCGCC | 1 cut(s) | 125 |
| PshAI | GACNNNNGTC | 1 cut(s) | 401 |
| Psp6I | CCWGG | 5 cut(s) | 89, 124, 163, 254, 287 |
| PspFI | CCCAGC | 2 cut(s) | 61, 345 |
| PspGI | CCWGG | 5 cut(s) | 89, 124, 163, 254, 287 |
| PspN4I | GGNNCC | 4 cut(s) | 123, 183, 422, 448 |
| PspPI | GGNCC | 4 cut(s) | 128, 182, 264, 446 |
| PstI | CTGCAG | 1 cut(s) | 471 |
| RsaI | GTAC | 2 cut(s) | 138, 150 |
| RsaNI | GTAC | 2 cut(s) | 137, 149 |
| SalI | GTCGAC | 1 cut(s) | 99 |
| SatI | GCNGC | 4 cut(s) | 6, 48, 85, 459 |
| Sau3AI | GATC | 3 cut(s) | 274, 384, 462 |
| Sau96I | GGNCC | 4 cut(s) | 128, 182, 264, 446 |
| SbfI | CCTGCAGG | 1 cut(s) | 471 |
| ScrFI | CCNGG | 8 cut(s) | 91, 126, 165, 256, 289, 389, 407, 444 |
| SdaI | CCTGCAGG | 1 cut(s) | 471 |
| SetI | ASST | 9 cut(s) | 22, 166, 199, 219, 274, 312, 351, 383, 403 |
| SfaNI | GCATC | 3 cut(s) | 325, 351, 419 |
| SfcI | CTRYAG | 1 cut(s) | 467 |
| SfoI | GGCGCC | 1 cut(s) | 123 |
| SinI | GGWCC | 2 cut(s) | 182, 264 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 471 |
| SspDI | GGCGCC | 1 cut(s) | 121 |
| SspMI | CTAG | 1 cut(s) | 417 |
| StyD4I | CCNGG | 8 cut(s) | 89, 124, 163, 254, 287, 387, 405, 442 |
| TaiI | ACGT | 1 cut(s) | 22 |
| TaqI | TCGA | 2 cut(s) | 100, 248 |
| TaqII | GACCGA | 2 cut(s) | 281, 496 |
| TauI | GCSGC | 2 cut(s) | 50, 461 |
| TfiI | GAWTC | 2 cut(s) | 224, 323 |
| TseI | GCWGC | 2 cut(s) | 5, 84 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 352 |
| VpaK11BI | GGWCC | 2 cut(s) | 182, 264 |
| XceI | RCATGY | 1 cut(s) | 149 |
| XmiI | GTMKAC | 1 cut(s) | 100 |
| XmnI | GAANNNNTTC | 1 cut(s) | 228 |
| XspI | CTAG | 1 cut(s) | 417 |
| Zsp2I | ATGCAT | 1 cut(s) | 147 |