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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 502 |
| Acc36I | ACCTGC | 2 cut(s) | 349, 502 |
| Acc65I | GGTACC | 2 cut(s) | 334, 361 |
| AccB1I | GGYRCC | 2 cut(s) | 334, 361 |
| AccBSI | CCGCTC | 1 cut(s) | 459 |
| AciI | CCGC | 9 cut(s) | 15, 38, 63, 327, 401, 412, 457, 505, 520 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 1 cut(s) | 216 |
| AcsI | RAATTY | 1 cut(s) | 233 |
| AcuI | CTGAAG | 2 cut(s) | 48, 501 |
| AfaI | GTAC | 3 cut(s) | 336, 342, 363 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 139, 323, 358, 418, 421, 504, 505 |
| AgsI | TTSAA | 2 cut(s) | 118, 247 |
| AjnI | CCWGG | 4 cut(s) | 146, 158, 405, 500 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 239, 271 |
| AluBI | AGCT | 2 cut(s) | 138, 511 |
| AluI | AGCT | 2 cut(s) | 138, 511 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 5 cut(s) | 195, 201, 216, 394, 408 |
| ApaI | GGGCCC | 1 cut(s) | 398 |
| ApeKI | GCWGC | 1 cut(s) | 517 |
| ApoI | RAATTY | 1 cut(s) | 233 |
| Asp718I | GGTACC | 2 cut(s) | 334, 361 |
| AspLEI | GCGC | 2 cut(s) | 280, 517 |
| AspS9I | GGNCC | 4 cut(s) | 202, 394, 395, 409 |
| AsuHPI | GGTGA | 1 cut(s) | 483 |
| BaeGI | GKGCMC | 1 cut(s) | 398 |
| BalI | TGGCCA | 1 cut(s) | 218 |
| BanI | GGYRCC | 2 cut(s) | 334, 361 |
| BanII | GRGCYC | 2 cut(s) | 154, 398 |
| BciT130I | CCWGG | 4 cut(s) | 148, 160, 407, 502 |
| BfaI | CTAG | 4 cut(s) | 89, 468, 474, 529 |
| BfuAI | ACCTGC | 2 cut(s) | 349, 502 |
| BisI | GCNGC | 1 cut(s) | 518 |
| BlsI | GCNGC | 1 cut(s) | 519 |
| Bme1390I | CCNGG | 4 cut(s) | 148, 160, 407, 502 |
| BmgT120I | GGNCC | 4 cut(s) | 202, 394, 395, 409 |
| BmiI | GGNNCC | 5 cut(s) | 144, 336, 363, 396, 524 |
| BmrFI | CCNGG | 4 cut(s) | 148, 160, 407, 502 |
| BsaJI | CCNNGG | 6 cut(s) | 146, 147, 158, 198, 405, 500 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 139, 323, 358, 418, 421, 504, 505 |
| Bse118I | RCCGGY | 1 cut(s) | 323 |
| BseBI | CCWGG | 4 cut(s) | 148, 160, 407, 502 |
| BseDI | CCNNGG | 6 cut(s) | 146, 147, 158, 198, 405, 500 |
| BseGI | GGATG | 2 cut(s) | 154, 348 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 139, 323, 358, 418, 421, 504, 505 |
| BseSI | GKGCMC | 1 cut(s) | 398 |
| BseYI | CCCAGC | 2 cut(s) | 134, 274 |
| BshFI | GGCC | 5 cut(s) | 197, 203, 218, 396, 410 |
| BshNI | GGYRCC | 2 cut(s) | 334, 361 |
| BsiSI | CCGG | 1 cut(s) | 324 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 139, 323, 358, 418, 421, 504, 505 |
| BsnI | GGCC | 5 cut(s) | 197, 203, 218, 396, 410 |
| Bsp120I | GGGCCC | 1 cut(s) | 394 |
| Bsp1286I | GDGCHC | 2 cut(s) | 154, 398 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 9 cut(s) | 15, 38, 63, 327, 401, 412, 457, 505, 520 |
| BspANI | GGCC | 5 cut(s) | 197, 203, 218, 396, 410 |
| BspLI | GGNNCC | 5 cut(s) | 144, 336, 363, 396, 524 |
| BspMI | ACCTGC | 2 cut(s) | 349, 502 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 334, 361 |
| BsrBI | CCGCTC | 1 cut(s) | 459 |
| BsrFI | RCCGGY | 1 cut(s) | 323 |
| BssAI | RCCGGY | 1 cut(s) | 323 |
| BssECI | CCNNGG | 6 cut(s) | 146, 147, 158, 198, 405, 500 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 4 cut(s) | 148, 160, 407, 502 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 287, 412, 513 |
| BstF5I | GGATG | 2 cut(s) | 154, 348 |
| BstHHI | GCGC | 2 cut(s) | 280, 517 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 260, 275, 286, 517 |
| BstNI | CCWGG | 4 cut(s) | 148, 160, 407, 502 |
| BstSCI | CCNGG | 4 cut(s) | 146, 158, 405, 500 |
| BstSLI | GKGCMC | 1 cut(s) | 398 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 73 |
| BsuRI | GGCC | 5 cut(s) | 197, 203, 218, 396, 410 |
| BtsCI | GGATG | 2 cut(s) | 154, 348 |
| BveI | ACCTGC | 2 cut(s) | 349, 502 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 287, 412, 513 |
| CfoI | GCGC | 2 cut(s) | 280, 517 |
| Cfr10I | RCCGGY | 1 cut(s) | 323 |
| Cfr13I | GGNCC | 4 cut(s) | 202, 394, 395, 409 |
| CseI | GACGC | 1 cut(s) | 192 |
| Csp6I | GTAC | 3 cut(s) | 335, 341, 362 |
| CviQI | GTAC | 3 cut(s) | 335, 341, 362 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 216 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 342 |
| Eco24I | GRGCYC | 2 cut(s) | 154, 398 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 501 |
| EcoRII | CCWGG | 4 cut(s) | 146, 158, 405, 500 |
| EcoT38I | GRGCYC | 2 cut(s) | 154, 398 |
| FauI | CCCGC | 4 cut(s) | 8, 70, 419, 498 |
| Fnu4HI | GCNGC | 1 cut(s) | 518 |
| FokI | GGATG | 2 cut(s) | 141, 335 |
| FriOI | GRGCYC | 2 cut(s) | 154, 398 |
| Fsp4HI | GCNGC | 1 cut(s) | 518 |
| FspBI | CTAG | 4 cut(s) | 89, 468, 474, 529 |
| GlaI | GCGC | 2 cut(s) | 279, 516 |
| GluI | GCNGC | 1 cut(s) | 518 |
| GsaI | CCCAGC | 2 cut(s) | 138, 278 |
| HaeIII | GGCC | 5 cut(s) | 197, 203, 218, 396, 410 |
| HapII | CCGG | 1 cut(s) | 324 |
| HgaI | GACGC | 1 cut(s) | 192 |
| HhaI | GCGC | 2 cut(s) | 280, 517 |
| Hin6I | GCGC | 2 cut(s) | 278, 515 |
| HinP1I | GCGC | 2 cut(s) | 278, 515 |
| HincII | GTYRAC | 3 cut(s) | 103, 130, 314 |
| HindII | GTYRAC | 3 cut(s) | 103, 130, 314 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 1 cut(s) | 324 |
| HphI | GGTGA | 1 cut(s) | 483 |
| Hpy166II | GTNNAC | 3 cut(s) | 103, 130, 314 |
| Hpy8I | GTNNAC | 3 cut(s) | 103, 130, 314 |
| Hpy99I | CGWCG | 1 cut(s) | 242 |
| HpyAV | CCTTC | 2 cut(s) | 187, 476 |
| HpyCH4IV | ACGT | 1 cut(s) | 307 |
| HpyCH4V | TGCA | 1 cut(s) | 358 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 260, 275, 286, 517 |
| HpySE526I | ACGT | 1 cut(s) | 307 |
| HspAI | GCGC | 2 cut(s) | 278, 515 |
| KpnI | GGTACC | 2 cut(s) | 338, 365 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 424, 508 |
| MaeI | CTAG | 4 cut(s) | 89, 468, 474, 529 |
| MaeII | ACGT | 1 cut(s) | 307 |
| MalI | GATC | 1 cut(s) | 42 |
| MbiI | CCGCTC | 1 cut(s) | 459 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MfeI | CAATTG | 1 cut(s) | 290 |
| MhlI | GDGCHC | 2 cut(s) | 154, 398 |
| MlsI | TGGCCA | 1 cut(s) | 218 |
| MluCI | AATT | 4 cut(s) | 233, 242, 290, 487 |
| MluNI | TGGCCA | 1 cut(s) | 218 |
| MmeI | TCCRAC | 1 cut(s) | 237 |
| MnlI | CCTC | 4 cut(s) | 167, 193, 253, 456 |
| Mox20I | TGGCCA | 1 cut(s) | 218 |
| MscI | TGGCCA | 1 cut(s) | 218 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| MslI | CAYNNNNRTG | 2 cut(s) | 224, 353 |
| Msp20I | TGGCCA | 1 cut(s) | 218 |
| MspA1I | CMGCKG | 1 cut(s) | 520 |
| MspI | CCGG | 1 cut(s) | 324 |
| MspR9I | CCNGG | 4 cut(s) | 148, 160, 407, 502 |
| MunI | CAATTG | 1 cut(s) | 290 |
| MvaI | CCWGG | 4 cut(s) | 148, 160, 407, 502 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 260, 275, 286, 517 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIV | GGNNCC | 5 cut(s) | 144, 336, 363, 396, 524 |
| NmeAIII | GCCGAG | 1 cut(s) | 223 |
| PaqCI | CACCTGC | 1 cut(s) | 502 |
| PasI | CCCWGGG | 1 cut(s) | 147 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PflFI | GACNNNGTC | 1 cut(s) | 309 |
| PkrI | GCNGC | 1 cut(s) | 519 |
| Psp6I | CCWGG | 4 cut(s) | 146, 158, 405, 500 |
| PspFI | CCCAGC | 2 cut(s) | 134, 274 |
| PspGI | CCWGG | 4 cut(s) | 146, 158, 405, 500 |
| PspN4I | GGNNCC | 5 cut(s) | 144, 336, 363, 396, 524 |
| PspOMI | GGGCCC | 1 cut(s) | 394 |
| PspPI | GGNCC | 4 cut(s) | 202, 394, 395, 409 |
| PsyI | GACNNNGTC | 1 cut(s) | 309 |
| RsaI | GTAC | 3 cut(s) | 336, 342, 363 |
| RsaNI | GTAC | 3 cut(s) | 335, 341, 362 |
| RseI | CAYNNNNRTG | 2 cut(s) | 224, 353 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 1 cut(s) | 518 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 4 cut(s) | 202, 394, 395, 409 |
| ScrFI | CCNGG | 4 cut(s) | 148, 160, 407, 502 |
| SduI | GDGCHC | 2 cut(s) | 154, 398 |
| SetI | ASST | 9 cut(s) | 90, 140, 310, 363, 448, 487, 496, 513, 528 |
| SgrAI | CRCCGGYG | 1 cut(s) | 323 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 224, 353 |
| Sse9I | AATT | 4 cut(s) | 233, 242, 290, 487 |
| SsiI | CCGC | 9 cut(s) | 15, 38, 63, 327, 401, 412, 457, 505, 520 |
| SspMI | CTAG | 4 cut(s) | 89, 468, 474, 529 |
| StyD4I | CCNGG | 4 cut(s) | 146, 158, 405, 500 |
| TaiI | ACGT | 1 cut(s) | 310 |
| TaqI | TCGA | 1 cut(s) | 237 |
| TaqII | GACCGA | 1 cut(s) | 115 |
| TasI | AATT | 4 cut(s) | 233, 242, 290, 487 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TseI | GCWGC | 1 cut(s) | 517 |
| Tth111I | GACNNNGTC | 1 cut(s) | 309 |
| XapI | RAATTY | 1 cut(s) | 233 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 508 |
| XspI | CTAG | 4 cut(s) | 89, 468, 474, 529 |