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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 226 |
| Acc36I | ACCTGC | 1 cut(s) | 226 |
| AccII | CGCG | 1 cut(s) | 198 |
| AciI | CCGC | 3 cut(s) | 48, 147, 170 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AcoI | YGGCCR | 1 cut(s) | 240 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 127, 235 |
| AgsI | TTSAA | 2 cut(s) | 118, 271 |
| AjnI | CCWGG | 1 cut(s) | 135 |
| AluBI | AGCT | 1 cut(s) | 160 |
| AluI | AGCT | 1 cut(s) | 160 |
| Alw21I | GWGCWC | 1 cut(s) | 112 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AoxI | GGCC | 3 cut(s) | 36, 219, 240 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| AspLEI | GCGC | 3 cut(s) | 198, 210, 280 |
| AspS9I | GGNCC | 3 cut(s) | 139, 172, 226 |
| AvaII | GGWCC | 3 cut(s) | 139, 172, 226 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BalI | TGGCCA | 1 cut(s) | 242 |
| Bbv12I | GWGCWC | 1 cut(s) | 112 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 137 |
| BfoI | RGCGCY | 1 cut(s) | 281 |
| BfuAI | ACCTGC | 1 cut(s) | 226 |
| BisI | GCNGC | 1 cut(s) | 147 |
| BlsI | GCNGC | 1 cut(s) | 148 |
| Bme1390I | CCNGG | 1 cut(s) | 137 |
| Bme18I | GGWCC | 3 cut(s) | 139, 172, 226 |
| BmgT120I | GGNCC | 3 cut(s) | 139, 172, 226 |
| BmiI | GGNNCC | 1 cut(s) | 132 |
| BmrFI | CCNGG | 1 cut(s) | 137 |
| BmrI | ACTGGG | 1 cut(s) | 83 |
| BmuI | ACTGGG | 1 cut(s) | 83 |
| BsaJI | CCNNGG | 2 cut(s) | 135, 222 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 127, 235 |
| Bse1I | ACTGG | 2 cut(s) | 89, 171 |
| BseBI | CCWGG | 1 cut(s) | 137 |
| BseDI | CCNNGG | 2 cut(s) | 135, 222 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 127, 235 |
| BseNI | ACTGG | 2 cut(s) | 89, 171 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseYI | CCCAGC | 2 cut(s) | 70, 298 |
| Bsh1236I | CGCG | 1 cut(s) | 198 |
| BshFI | GGCC | 3 cut(s) | 38, 221, 242 |
| BsiHKAI | GWGCWC | 1 cut(s) | 112 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 127, 235 |
| BsnI | GGCC | 3 cut(s) | 38, 221, 242 |
| Bsp1286I | GDGCHC | 1 cut(s) | 112 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 3 cut(s) | 48, 147, 170 |
| BspANI | GGCC | 3 cut(s) | 38, 221, 242 |
| BspFNI | CGCG | 1 cut(s) | 198 |
| BspLI | GGNNCC | 1 cut(s) | 132 |
| BspMI | ACCTGC | 1 cut(s) | 226 |
| BsrI | ACTGG | 2 cut(s) | 89, 171 |
| BssECI | CCNNGG | 2 cut(s) | 135, 222 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 1 cut(s) | 137 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 107 |
| BstC8I | GCNNGC | 2 cut(s) | 162, 200 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 1 cut(s) | 198 |
| BstH2I | RGCGCY | 1 cut(s) | 281 |
| BstHHI | GCGC | 3 cut(s) | 198, 210, 280 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 284, 299 |
| BstNI | CCWGG | 1 cut(s) | 137 |
| BstSCI | CCNGG | 1 cut(s) | 135 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 1 cut(s) | 198 |
| BsuRI | GGCC | 3 cut(s) | 38, 221, 242 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 2 cut(s) | 96, 164 |
| BveI | ACCTGC | 1 cut(s) | 226 |
| Cac8I | GCNNGC | 2 cut(s) | 162, 200 |
| CfoI | GCGC | 3 cut(s) | 198, 210, 280 |
| Cfr13I | GGNCC | 3 cut(s) | 139, 172, 226 |
| CviJI | RGCY | 4 cut(s) | 38, 160, 221, 242 |
| CviKI_1 | RGCY | 4 cut(s) | 38, 160, 221, 242 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 240 |
| EciI | GGCGGA | 2 cut(s) | 37, 185 |
| Eco47I | GGWCC | 3 cut(s) | 139, 172, 226 |
| EcoO109I | RGGNCCY | 1 cut(s) | 139 |
| EcoRII | CCWGG | 1 cut(s) | 135 |
| FaiI | YATR | 3 cut(s) | 128, 152, 156 |
| Fnu4HI | GCNGC | 1 cut(s) | 147 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 1 cut(s) | 147 |
| GlaI | GCGC | 3 cut(s) | 197, 209, 279 |
| GluI | GCNGC | 1 cut(s) | 147 |
| GsaI | CCCAGC | 2 cut(s) | 74, 302 |
| HaeII | RGCGCY | 1 cut(s) | 281 |
| HaeIII | GGCC | 3 cut(s) | 38, 221, 242 |
| HhaI | GCGC | 3 cut(s) | 198, 210, 280 |
| Hin6I | GCGC | 3 cut(s) | 196, 208, 278 |
| HinP1I | GCGC | 3 cut(s) | 196, 208, 278 |
| HincII | GTYRAC | 3 cut(s) | 85, 97, 103 |
| HindII | GTYRAC | 3 cut(s) | 85, 97, 103 |
| HpaI | GTTAAC | 3 cut(s) | 85, 97, 103 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 97, 103, 110 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 97, 103, 110 |
| HpyAV | CCTTC | 2 cut(s) | 185, 268 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 107 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 1 cut(s) | 110 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 284, 299 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| HspAI | GCGC | 3 cut(s) | 196, 208, 278 |
| KspAI | GTTAAC | 3 cut(s) | 85, 97, 103 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LmnI | GCTCC | 1 cut(s) | 205 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MalI | GATC | 1 cut(s) | 42 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 1 cut(s) | 283 |
| MhlI | GDGCHC | 1 cut(s) | 112 |
| MlsI | TGGCCA | 1 cut(s) | 242 |
| MluNI | TGGCCA | 1 cut(s) | 242 |
| MnlI | CCTC | 1 cut(s) | 217 |
| Mox20I | TGGCCA | 1 cut(s) | 242 |
| MscI | TGGCCA | 1 cut(s) | 242 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 254 |
| MslI | CAYNNNNRTG | 1 cut(s) | 248 |
| Msp20I | TGGCCA | 1 cut(s) | 242 |
| MspR9I | CCNGG | 1 cut(s) | 137 |
| MvaI | CCWGG | 1 cut(s) | 137 |
| MvnI | CGCG | 1 cut(s) | 198 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 284, 299 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIV | GGNNCC | 1 cut(s) | 132 |
| NmeAIII | GCCGAG | 1 cut(s) | 247 |
| PaqCI | CACCTGC | 1 cut(s) | 226 |
| PkrI | GCNGC | 1 cut(s) | 148 |
| PpuMI | RGGWCCY | 1 cut(s) | 139 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp5II | RGGWCCY | 1 cut(s) | 139 |
| Psp6I | CCWGG | 1 cut(s) | 135 |
| PspFI | CCCAGC | 2 cut(s) | 70, 298 |
| PspGI | CCWGG | 1 cut(s) | 135 |
| PspN4I | GGNNCC | 1 cut(s) | 132 |
| PspPI | GGNCC | 3 cut(s) | 139, 172, 226 |
| PspPPI | RGGWCCY | 1 cut(s) | 139 |
| RseI | CAYNNNNRTG | 1 cut(s) | 248 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 254 |
| SatI | GCNGC | 1 cut(s) | 147 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 3 cut(s) | 139, 172, 226 |
| ScrFI | CCNGG | 1 cut(s) | 137 |
| SduI | GDGCHC | 1 cut(s) | 112 |
| SetI | ASST | 7 cut(s) | 84, 102, 144, 162, 177, 228, 240 |
| SinI | GGWCC | 3 cut(s) | 139, 172, 226 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 248 |
| SsiI | CCGC | 3 cut(s) | 48, 147, 170 |
| StyD4I | CCNGG | 1 cut(s) | 135 |
| TaaI | ACNGT | 2 cut(s) | 11, 107 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TauI | GCSGC | 1 cut(s) | 149 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 254 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 254 |
| TscAI | CASTG | 2 cut(s) | 96, 171 |
| TspRI | CASTG | 2 cut(s) | 96, 171 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 3 cut(s) | 139, 172, 226 |