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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 120 |
| Acc36I | ACCTGC | 1 cut(s) | 120 |
| AccBSI | CCGCTC | 2 cut(s) | 267, 305 |
| AccII | CGCG | 2 cut(s) | 54, 56 |
| AclWI | GGATC | 1 cut(s) | 325 |
| AcoI | YGGCCR | 1 cut(s) | 134 |
| AfaI | GTAC | 1 cut(s) | 334 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 41, 129, 241, 309, 314 |
| AjnI | CCWGG | 3 cut(s) | 191, 220, 234 |
| AleI | CACNNNNGTG | 1 cut(s) | 142 |
| AluBI | AGCT | 1 cut(s) | 230 |
| AluI | AGCT | 1 cut(s) | 230 |
| Alw26I | GTCTC | 1 cut(s) | 265 |
| AlwI | GGATC | 1 cut(s) | 325 |
| Ama87I | CYCGRG | 1 cut(s) | 268 |
| AoxI | GGCC | 4 cut(s) | 113, 119, 134, 232 |
| AspLEI | GCGC | 2 cut(s) | 56, 58 |
| AspS9I | GGNCC | 2 cut(s) | 120, 343 |
| AsuC2I | CCSGG | 2 cut(s) | 81, 337 |
| AvaI | CYCGRG | 1 cut(s) | 268 |
| AvaII | GGWCC | 1 cut(s) | 343 |
| AxyI | CCTNAGG | 1 cut(s) | 276 |
| BalI | TGGCCA | 1 cut(s) | 136 |
| BccI | CCATC | 1 cut(s) | 23 |
| BciT130I | CCWGG | 3 cut(s) | 193, 222, 236 |
| BcnI | CCSGG | 2 cut(s) | 81, 337 |
| BcoDI | GTCTC | 1 cut(s) | 265 |
| BfuAI | ACCTGC | 1 cut(s) | 120 |
| Bme1390I | CCNGG | 5 cut(s) | 81, 193, 222, 236, 337 |
| Bme18I | GGWCC | 1 cut(s) | 343 |
| BmeT110I | CYCGRG | 1 cut(s) | 268 |
| BmgT120I | GGNCC | 2 cut(s) | 120, 343 |
| BmiI | GGNNCC | 1 cut(s) | 345 |
| BmrFI | CCNGG | 5 cut(s) | 81, 193, 222, 236, 337 |
| BmsI | GCATC | 1 cut(s) | 54 |
| BpmI | CTGGAG | 1 cut(s) | 257 |
| BpuMI | CCSGG | 2 cut(s) | 81, 337 |
| BsaI | GGTCTC | 1 cut(s) | 265 |
| BsaJI | CCNNGG | 3 cut(s) | 79, 116, 308 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 230, 260 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 41, 129, 241, 309, 314 |
| Bse118I | RCCGGY | 1 cut(s) | 350 |
| Bse21I | CCTNAGG | 1 cut(s) | 276 |
| BseBI | CCWGG | 3 cut(s) | 193, 222, 236 |
| BseDI | CCNNGG | 3 cut(s) | 79, 116, 308 |
| BseGI | GGATG | 1 cut(s) | 15 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 41, 129, 241, 309, 314 |
| BseMII | CTCAG | 1 cut(s) | 290 |
| BsePI | GCGCGC | 1 cut(s) | 54 |
| BseYI | CCCAGC | 1 cut(s) | 34 |
| BsgI | GTGCAG | 1 cut(s) | 239 |
| Bsh1236I | CGCG | 2 cut(s) | 54, 56 |
| BshFI | GGCC | 4 cut(s) | 115, 121, 136, 234 |
| BsiHKCI | CYCGRG | 1 cut(s) | 268 |
| BsiSI | CCGG | 3 cut(s) | 81, 337, 351 |
| BslFI | GGGAC | 1 cut(s) | 132 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 41, 129, 241, 309, 314 |
| BsmAI | GTCTC | 1 cut(s) | 265 |
| BsmFI | GGGAC | 1 cut(s) | 132 |
| BsnI | GGCC | 4 cut(s) | 115, 121, 136, 234 |
| Bso31I | GGTCTC | 1 cut(s) | 265 |
| BsoBI | CYCGRG | 1 cut(s) | 268 |
| Bsp143I | GATC | 3 cut(s) | 4, 72, 317 |
| BspANI | GGCC | 4 cut(s) | 115, 121, 136, 234 |
| BspCNI | CTCAG | 1 cut(s) | 289 |
| BspFNI | CGCG | 2 cut(s) | 54, 56 |
| BspLI | GGNNCC | 1 cut(s) | 345 |
| BspMI | ACCTGC | 1 cut(s) | 120 |
| BspPI | GGATC | 1 cut(s) | 325 |
| BspTNI | GGTCTC | 1 cut(s) | 265 |
| BsrBI | CCGCTC | 2 cut(s) | 267, 305 |
| BsrFI | RCCGGY | 1 cut(s) | 350 |
| BssAI | RCCGGY | 1 cut(s) | 350 |
| BssECI | CCNNGG | 3 cut(s) | 79, 116, 308 |
| BssHII | GCGCGC | 1 cut(s) | 54 |
| BssMI | GATC | 3 cut(s) | 4, 72, 317 |
| Bst2UI | CCWGG | 3 cut(s) | 193, 222, 236 |
| BstC8I | GCNNGC | 3 cut(s) | 56, 205, 232 |
| BstDEI | CTNAG | 1 cut(s) | 276 |
| BstF5I | GGATG | 1 cut(s) | 15 |
| BstFNI | CGCG | 2 cut(s) | 54, 56 |
| BstHHI | GCGC | 2 cut(s) | 56, 58 |
| BstKTI | GATC | 3 cut(s) | 7, 75, 320 |
| BstMAI | GTCTC | 1 cut(s) | 265 |
| BstMBI | GATC | 3 cut(s) | 4, 72, 317 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 51, 204, 231 |
| BstNI | CCWGG | 3 cut(s) | 193, 222, 236 |
| BstSCI | CCNGG | 5 cut(s) | 79, 191, 220, 234, 335 |
| BstUI | CGCG | 2 cut(s) | 54, 56 |
| Bsu36I | CCTNAGG | 1 cut(s) | 276 |
| BsuRI | GGCC | 4 cut(s) | 115, 121, 136, 234 |
| BtsCI | GGATG | 1 cut(s) | 15 |
| BtsI | GCAGTG | 1 cut(s) | 251 |
| BtsIMutI | CAGTG | 1 cut(s) | 251 |
| BveI | ACCTGC | 1 cut(s) | 120 |
| Cac8I | GCNNGC | 3 cut(s) | 56, 205, 232 |
| CfoI | GCGC | 2 cut(s) | 56, 58 |
| Cfr10I | RCCGGY | 1 cut(s) | 350 |
| Cfr13I | GGNCC | 2 cut(s) | 120, 343 |
| CsiI | ACCWGGT | 1 cut(s) | 191 |
| Csp6I | GTAC | 1 cut(s) | 333 |
| CviJI | RGCY | 7 cut(s) | 115, 121, 136, 181, 230, 234, 340 |
| CviKI_1 | RGCY | 7 cut(s) | 115, 121, 136, 181, 230, 234, 340 |
| CviQI | GTAC | 1 cut(s) | 333 |
| DdeI | CTNAG | 1 cut(s) | 276 |
| DpnI | GATC | 3 cut(s) | 6, 74, 319 |
| DpnII | GATC | 3 cut(s) | 4, 72, 317 |
| EaeI | YGGCCR | 1 cut(s) | 134 |
| EciI | GGCGGA | 1 cut(s) | 257 |
| Eco31I | GGTCTC | 1 cut(s) | 265 |
| Eco47I | GGWCC | 1 cut(s) | 343 |
| Eco81I | CCTNAGG | 1 cut(s) | 276 |
| Eco88I | CYCGRG | 1 cut(s) | 268 |
| EcoRII | CCWGG | 3 cut(s) | 191, 220, 234 |
| FaqI | GGGAC | 1 cut(s) | 132 |
| FauI | CCCGC | 2 cut(s) | 77, 310 |
| FokI | GGATG | 1 cut(s) | 2 |
| GlaI | GCGC | 2 cut(s) | 55, 57 |
| GsaI | CCCAGC | 1 cut(s) | 38 |
| GsuI | CTGGAG | 1 cut(s) | 257 |
| HaeIII | GGCC | 4 cut(s) | 115, 121, 136, 234 |
| HapII | CCGG | 3 cut(s) | 81, 337, 351 |
| HhaI | GCGC | 2 cut(s) | 56, 58 |
| Hin6I | GCGC | 2 cut(s) | 54, 56 |
| HinP1I | GCGC | 2 cut(s) | 54, 56 |
| HinfI | GANTC | 1 cut(s) | 245 |
| HpaII | CCGG | 3 cut(s) | 81, 337, 351 |
| Hpy166II | GTNNAC | 1 cut(s) | 94 |
| Hpy188I | TCNGA | 1 cut(s) | 309 |
| Hpy188III | TCNNGA | 1 cut(s) | 270 |
| Hpy8I | GTNNAC | 1 cut(s) | 94 |
| HpyCH4V | TGCA | 1 cut(s) | 256 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 51, 204, 231 |
| HpyF3I | CTNAG | 1 cut(s) | 276 |
| HspAI | GCGC | 2 cut(s) | 54, 56 |
| Kzo9I | GATC | 3 cut(s) | 4, 72, 317 |
| LmnI | GCTCC | 3 cut(s) | 87, 200, 310 |
| LweI | GCATC | 1 cut(s) | 54 |
| MabI | ACCWGGT | 1 cut(s) | 191 |
| MalI | GATC | 3 cut(s) | 6, 74, 319 |
| MbiI | CCGCTC | 2 cut(s) | 267, 305 |
| MboI | GATC | 3 cut(s) | 4, 72, 317 |
| MlsI | TGGCCA | 1 cut(s) | 136 |
| MluNI | TGGCCA | 1 cut(s) | 136 |
| MlyI | GAGTC | 1 cut(s) | 254 |
| MnlI | CCTC | 5 cut(s) | 111, 232, 283, 285, 303 |
| Mox20I | TGGCCA | 1 cut(s) | 136 |
| MscI | TGGCCA | 1 cut(s) | 136 |
| MseI | TTAA | 2 cut(s) | 22, 163 |
| MslI | CAYNNNNRTG | 1 cut(s) | 142 |
| Msp20I | TGGCCA | 1 cut(s) | 136 |
| MspA1I | CMGCKG | 3 cut(s) | 38, 230, 328 |
| MspI | CCGG | 3 cut(s) | 81, 337, 351 |
| MspR9I | CCNGG | 5 cut(s) | 81, 193, 222, 236, 337 |
| MvaI | CCWGG | 3 cut(s) | 193, 222, 236 |
| MvnI | CGCG | 2 cut(s) | 54, 56 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 51, 204, 231 |
| NciI | CCSGG | 2 cut(s) | 81, 337 |
| NdeII | GATC | 3 cut(s) | 4, 72, 317 |
| NlaIV | GGNNCC | 1 cut(s) | 345 |
| NmeAIII | GCCGAG | 1 cut(s) | 141 |
| OliI | CACNNNNGTG | 1 cut(s) | 142 |
| PaeR7I | CTCGAG | 1 cut(s) | 268 |
| PaqCI | CACCTGC | 1 cut(s) | 120 |
| PauI | GCGCGC | 1 cut(s) | 54 |
| PleI | GAGTC | 1 cut(s) | 253 |
| PpsI | GAGTC | 1 cut(s) | 253 |
| Psp6I | CCWGG | 3 cut(s) | 191, 220, 234 |
| PspFI | CCCAGC | 1 cut(s) | 34 |
| PspGI | CCWGG | 3 cut(s) | 191, 220, 234 |
| PspN4I | GGNNCC | 1 cut(s) | 345 |
| PspPI | GGNCC | 2 cut(s) | 120, 343 |
| PteI | GCGCGC | 1 cut(s) | 54 |
| PvuII | CAGCTG | 1 cut(s) | 230 |
| RsaI | GTAC | 1 cut(s) | 334 |
| RsaNI | GTAC | 1 cut(s) | 333 |
| RseI | CAYNNNNRTG | 1 cut(s) | 142 |
| SaqAI | TTAA | 2 cut(s) | 22, 163 |
| Sau3AI | GATC | 3 cut(s) | 4, 72, 317 |
| Sau96I | GGNCC | 2 cut(s) | 120, 343 |
| SchI | GAGTC | 1 cut(s) | 254 |
| ScrFI | CCNGG | 5 cut(s) | 81, 193, 222, 236, 337 |
| SetI | ASST | 4 cut(s) | 134, 197, 232, 277 |
| SexAI | ACCWGGT | 1 cut(s) | 191 |
| SfaNI | GCATC | 1 cut(s) | 54 |
| Sfr274I | CTCGAG | 1 cut(s) | 268 |
| SgrAI | CRCCGGYG | 1 cut(s) | 350 |
| SinI | GGWCC | 1 cut(s) | 343 |
| SlaI | CTCGAG | 1 cut(s) | 268 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 142 |
| SmlI | CTYRAG | 1 cut(s) | 268 |
| SmoI | CTYRAG | 1 cut(s) | 268 |
| StyD4I | CCNGG | 5 cut(s) | 79, 191, 220, 234, 335 |
| TaqI | TCGA | 1 cut(s) | 269 |
| Tru1I | TTAA | 2 cut(s) | 22, 163 |
| Tru9I | TTAA | 2 cut(s) | 22, 163 |
| TscAI | CASTG | 1 cut(s) | 258 |
| TspRI | CASTG | 1 cut(s) | 258 |
| VpaK11BI | GGWCC | 1 cut(s) | 343 |
| XhoI | CTCGAG | 1 cut(s) | 268 |