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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 47 |
| AccBSI | CCGCTC | 1 cut(s) | 214 |
| AccII | CGCG | 1 cut(s) | 90 |
| AciI | CCGC | 6 cut(s) | 15, 74, 165, 188, 214, 363 |
| AcuI | CTGAAG | 1 cut(s) | 261 |
| AdeI | CACNNNGTG | 1 cut(s) | 341 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 199 |
| AgsI | TTSAA | 1 cut(s) | 100 |
| AjnI | CCWGG | 3 cut(s) | 129, 141, 153 |
| AluBI | AGCT | 2 cut(s) | 178, 245 |
| AluI | AGCT | 2 cut(s) | 178, 245 |
| AspLEI | GCGC | 2 cut(s) | 92, 298 |
| AspS9I | GGNCC | 2 cut(s) | 157, 190 |
| AvaII | GGWCC | 2 cut(s) | 157, 190 |
| BanII | GRGCYC | 1 cut(s) | 129 |
| BccI | CCATC | 2 cut(s) | 23, 395 |
| BciT130I | CCWGG | 3 cut(s) | 131, 143, 155 |
| BciVI | GTATCC | 1 cut(s) | 127 |
| BfaI | CTAG | 3 cut(s) | 299, 345, 360 |
| BfoI | RGCGCY | 1 cut(s) | 299 |
| BfuI | GTATCC | 1 cut(s) | 127 |
| BisI | GCNGC | 2 cut(s) | 75, 165 |
| BlsI | GCNGC | 2 cut(s) | 76, 166 |
| Bme1390I | CCNGG | 3 cut(s) | 131, 143, 155 |
| Bme18I | GGWCC | 2 cut(s) | 157, 190 |
| BmgT120I | GGNCC | 2 cut(s) | 157, 190 |
| BmiI | GGNNCC | 1 cut(s) | 147 |
| BmrFI | CCNGG | 3 cut(s) | 131, 143, 155 |
| BmrI | ACTGGG | 1 cut(s) | 111 |
| BmuI | ACTGGG | 1 cut(s) | 111 |
| BpmI | CTGGAG | 1 cut(s) | 90 |
| BsaJI | CCNNGG | 2 cut(s) | 129, 141 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 199 |
| Bse1I | ACTGG | 4 cut(s) | 43, 107, 117, 189 |
| BseBI | CCWGG | 3 cut(s) | 131, 143, 155 |
| BseDI | CCNNGG | 2 cut(s) | 129, 141 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 199 |
| BseMII | CTCAG | 1 cut(s) | 260 |
| BseNI | ACTGG | 4 cut(s) | 43, 107, 117, 189 |
| BseYI | CCCAGC | 1 cut(s) | 316 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| Bsh1285I | CGRYCG | 1 cut(s) | 367 |
| BsiEI | CGRYCG | 1 cut(s) | 367 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 199 |
| Bsp1286I | GDGCHC | 1 cut(s) | 129 |
| BspACI | CCGC | 6 cut(s) | 15, 74, 165, 188, 214, 363 |
| BspCNI | CTCAG | 1 cut(s) | 259 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspLI | GGNNCC | 1 cut(s) | 147 |
| BsrBI | CCGCTC | 1 cut(s) | 214 |
| BsrI | ACTGG | 4 cut(s) | 43, 107, 117, 189 |
| BssECI | CCNNGG | 2 cut(s) | 129, 141 |
| Bst2UI | CCWGG | 3 cut(s) | 131, 143, 155 |
| Bst6I | CTCTTC | 1 cut(s) | 101 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 180 |
| BstDEI | CTNAG | 1 cut(s) | 246 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 197 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 1 cut(s) | 299 |
| BstHHI | GCGC | 2 cut(s) | 92, 298 |
| BstMCI | CGRYCG | 1 cut(s) | 367 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 302, 317 |
| BstNI | CCWGG | 3 cut(s) | 131, 143, 155 |
| BstSCI | CCNGG | 3 cut(s) | 129, 141, 153 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BsuI | GTATCC | 1 cut(s) | 127 |
| BtsIMutI | CAGTG | 2 cut(s) | 124, 182 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 180 |
| CfoI | GCGC | 2 cut(s) | 92, 298 |
| Cfr13I | GGNCC | 2 cut(s) | 157, 190 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 52, 87 |
| CviJI | RGCY | 6 cut(s) | 77, 127, 178, 239, 245, 260 |
| CviKI_1 | RGCY | 6 cut(s) | 77, 127, 178, 239, 245, 260 |
| DdeI | CTNAG | 1 cut(s) | 246 |
| DraIII | CACNNNGTG | 1 cut(s) | 341 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 47 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 47 |
| Eam1104I | CTCTTC | 1 cut(s) | 101 |
| EarI | CTCTTC | 1 cut(s) | 101 |
| EciI | GGCGGA | 1 cut(s) | 203 |
| Eco24I | GRGCYC | 1 cut(s) | 129 |
| Eco47I | GGWCC | 2 cut(s) | 157, 190 |
| Eco57I | CTGAAG | 1 cut(s) | 261 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 197 |
| EcoO109I | RGGNCCY | 1 cut(s) | 157 |
| EcoRII | CCWGG | 3 cut(s) | 129, 141, 153 |
| EcoT38I | GRGCYC | 1 cut(s) | 129 |
| FaiI | YATR | 3 cut(s) | 174, 392, 394 |
| FauI | CCCGC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 2 cut(s) | 75, 165 |
| FriOI | GRGCYC | 1 cut(s) | 129 |
| Fsp4HI | GCNGC | 2 cut(s) | 75, 165 |
| FspBI | CTAG | 3 cut(s) | 299, 345, 360 |
| GlaI | GCGC | 2 cut(s) | 91, 297 |
| GluI | GCNGC | 2 cut(s) | 75, 165 |
| GsaI | CCCAGC | 1 cut(s) | 320 |
| GsuI | CTGGAG | 1 cut(s) | 90 |
| HaeII | RGCGCY | 1 cut(s) | 299 |
| HhaI | GCGC | 2 cut(s) | 92, 298 |
| Hin6I | GCGC | 2 cut(s) | 90, 296 |
| HinP1I | GCGC | 2 cut(s) | 90, 296 |
| HincII | GTYRAC | 3 cut(s) | 52, 112, 356 |
| HindII | GTYRAC | 3 cut(s) | 52, 112, 356 |
| Hpy166II | GTNNAC | 3 cut(s) | 52, 112, 356 |
| Hpy188I | TCNGA | 1 cut(s) | 249 |
| Hpy188III | TCNNGA | 1 cut(s) | 273 |
| Hpy8I | GTNNAC | 3 cut(s) | 52, 112, 356 |
| HpyAV | CCTTC | 2 cut(s) | 286, 363 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 302, 317 |
| HpyF3I | CTNAG | 1 cut(s) | 246 |
| HspAI | GCGC | 2 cut(s) | 90, 296 |
| LmnI | GCTCC | 1 cut(s) | 211 |
| MaeI | CTAG | 3 cut(s) | 299, 345, 360 |
| MbiI | CCGCTC | 1 cut(s) | 214 |
| MboII | GAAGA | 1 cut(s) | 88 |
| MhlI | GDGCHC | 1 cut(s) | 129 |
| MnlI | CCTC | 3 cut(s) | 203, 295, 318 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 266 |
| MspR9I | CCNGG | 3 cut(s) | 131, 143, 155 |
| MvaI | CCWGG | 3 cut(s) | 131, 143, 155 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 302, 317 |
| NlaIV | GGNNCC | 1 cut(s) | 147 |
| PkrI | GCNGC | 2 cut(s) | 76, 166 |
| PpuMI | RGGWCCY | 1 cut(s) | 157 |
| Psp5II | RGGWCCY | 1 cut(s) | 157 |
| Psp6I | CCWGG | 3 cut(s) | 129, 141, 153 |
| PspFI | CCCAGC | 1 cut(s) | 316 |
| PspGI | CCWGG | 3 cut(s) | 129, 141, 153 |
| PspN4I | GGNNCC | 1 cut(s) | 147 |
| PspPI | GGNCC | 2 cut(s) | 157, 190 |
| PspPPI | RGGWCCY | 1 cut(s) | 157 |
| RseI | CAYNNNNRTG | 1 cut(s) | 266 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 2 cut(s) | 75, 165 |
| Sau96I | GGNCC | 2 cut(s) | 157, 190 |
| ScrFI | CCNGG | 3 cut(s) | 131, 143, 155 |
| SduI | GDGCHC | 1 cut(s) | 129 |
| SetI | ASST | 8 cut(s) | 72, 147, 162, 180, 195, 247, 361, 374 |
| SinI | GGWCC | 2 cut(s) | 157, 190 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 266 |
| SsiI | CCGC | 6 cut(s) | 15, 74, 165, 188, 214, 363 |
| SspMI | CTAG | 3 cut(s) | 299, 345, 360 |
| StyD4I | CCNGG | 3 cut(s) | 129, 141, 153 |
| TaqI | TCGA | 2 cut(s) | 123, 367 |
| TauI | GCSGC | 2 cut(s) | 77, 167 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 124, 189 |
| TspDTI | ATGAA | 1 cut(s) | 297 |
| TspGWI | ACGGA | 1 cut(s) | 37 |
| TspRI | CASTG | 2 cut(s) | 124, 189 |
| VpaK11BI | GGWCC | 2 cut(s) | 157, 190 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 197 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 175 |
| XspI | CTAG | 3 cut(s) | 299, 345, 360 |