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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 265 |
| Acc65I | GGTACC | 2 cut(s) | 250, 277 |
| AccB1I | GGYRCC | 2 cut(s) | 250, 277 |
| AccBSI | CCGCTC | 2 cut(s) | 34, 375 |
| AccI | GTMKAC | 1 cut(s) | 229 |
| AciI | CCGC | 9 cut(s) | 15, 34, 48, 74, 187, 362, 373, 407, 436 |
| AcuI | CTGAAG | 3 cut(s) | 48, 135, 417 |
| AfaI | GTAC | 4 cut(s) | 217, 252, 279, 414 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 127, 239, 379, 390 |
| AflIII | ACRYGT | 1 cut(s) | 146 |
| AjnI | CCWGG | 3 cut(s) | 218, 253, 383 |
| AoxI | GGCC | 3 cut(s) | 177, 257, 381 |
| Asp718I | GGTACC | 2 cut(s) | 250, 277 |
| AspLEI | GCGC | 2 cut(s) | 102, 423 |
| AspS9I | GGNCC | 2 cut(s) | 257, 311 |
| AsuC2I | CCSGG | 1 cut(s) | 433 |
| AsuHPI | GGTGA | 1 cut(s) | 142 |
| AvaII | GGWCC | 1 cut(s) | 311 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 234, 267 |
| BanI | GGYRCC | 2 cut(s) | 250, 277 |
| BbsI | GAAGAC | 1 cut(s) | 173 |
| BccI | CCATC | 3 cut(s) | 51, 59, 322 |
| BciT130I | CCWGG | 3 cut(s) | 220, 255, 385 |
| BcnI | CCSGG | 1 cut(s) | 433 |
| BfuAI | ACCTGC | 1 cut(s) | 265 |
| BisI | GCNGC | 1 cut(s) | 75 |
| BlsI | GCNGC | 1 cut(s) | 76 |
| Bme1390I | CCNGG | 4 cut(s) | 220, 255, 385, 433 |
| Bme18I | GGWCC | 1 cut(s) | 311 |
| BmgT120I | GGNCC | 2 cut(s) | 257, 311 |
| BmiI | GGNNCC | 3 cut(s) | 252, 279, 312 |
| BmrFI | CCNGG | 4 cut(s) | 220, 255, 385, 433 |
| BmsI | GCATC | 1 cut(s) | 261 |
| BpiI | GAAGAC | 1 cut(s) | 173 |
| BpmI | CTGGAG | 1 cut(s) | 194 |
| BpuMI | CCSGG | 1 cut(s) | 433 |
| BsaJI | CCNNGG | 3 cut(s) | 254, 384, 416 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 127, 239, 379, 390 |
| Bse118I | RCCGGY | 2 cut(s) | 239, 259 |
| Bse1I | ACTGG | 1 cut(s) | 177 |
| BseBI | CCWGG | 3 cut(s) | 220, 255, 385 |
| BseDI | CCNNGG | 3 cut(s) | 254, 384, 416 |
| BseGI | GGATG | 2 cut(s) | 43, 51 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 127, 239, 379, 390 |
| BseMII | CTCAG | 1 cut(s) | 285 |
| BseNI | ACTGG | 1 cut(s) | 177 |
| BseRI | GAGGAG | 2 cut(s) | 107, 110 |
| BseYI | CCCAGC | 3 cut(s) | 70, 190, 233 |
| Bsh1285I | CGRYCG | 1 cut(s) | 187 |
| BshFI | GGCC | 3 cut(s) | 179, 259, 383 |
| BshNI | GGYRCC | 2 cut(s) | 250, 277 |
| BsiEI | CGRYCG | 1 cut(s) | 187 |
| BsiSI | CCGG | 3 cut(s) | 240, 260, 433 |
| BslFI | GGGAC | 2 cut(s) | 324, 415 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 127, 239, 379, 390 |
| BsmFI | GGGAC | 2 cut(s) | 324, 415 |
| BsnI | GGCC | 3 cut(s) | 179, 259, 383 |
| BspACI | CCGC | 9 cut(s) | 15, 34, 48, 74, 187, 362, 373, 407, 436 |
| BspANI | GGCC | 3 cut(s) | 179, 259, 383 |
| BspCNI | CTCAG | 1 cut(s) | 286 |
| BspHI | TCATGA | 1 cut(s) | 141 |
| BspLI | GGNNCC | 3 cut(s) | 252, 279, 312 |
| BspMI | ACCTGC | 1 cut(s) | 265 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 250, 277 |
| BsrBI | CCGCTC | 2 cut(s) | 34, 375 |
| BsrFI | RCCGGY | 2 cut(s) | 239, 259 |
| BsrI | ACTGG | 1 cut(s) | 177 |
| BssAI | RCCGGY | 2 cut(s) | 239, 259 |
| BssECI | CCNNGG | 3 cut(s) | 254, 384, 416 |
| Bst2UI | CCWGG | 3 cut(s) | 220, 255, 385 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 203, 425 |
| BstDEI | CTNAG | 2 cut(s) | 212, 294 |
| BstF5I | GGATG | 2 cut(s) | 43, 51 |
| BstHHI | GCGC | 2 cut(s) | 102, 423 |
| BstMCI | CGRYCG | 1 cut(s) | 187 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 433 |
| BstNI | CCWGG | 3 cut(s) | 220, 255, 385 |
| BstNSI | RCATGY | 1 cut(s) | 150 |
| BstSCI | CCNGG | 4 cut(s) | 218, 253, 383, 431 |
| BstV2I | GAAGAC | 1 cut(s) | 173 |
| BsuRI | GGCC | 3 cut(s) | 179, 259, 383 |
| BtgZI | GCGATG | 1 cut(s) | 300 |
| BtsCI | GGATG | 2 cut(s) | 43, 51 |
| BtsIMutI | CAGTG | 2 cut(s) | 170, 281 |
| BveI | ACCTGC | 1 cut(s) | 265 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 203, 425 |
| CciI | TCATGA | 1 cut(s) | 141 |
| CfoI | GCGC | 2 cut(s) | 102, 423 |
| Cfr10I | RCCGGY | 2 cut(s) | 239, 259 |
| Cfr13I | GGNCC | 2 cut(s) | 257, 311 |
| Csp6I | GTAC | 4 cut(s) | 216, 251, 278, 413 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 279, 314 |
| CviAII | CATG | 2 cut(s) | 142, 147 |
| CviJI | RGCY | 6 cut(s) | 113, 119, 179, 259, 383, 427 |
| CviKI_1 | RGCY | 6 cut(s) | 113, 119, 179, 259, 383, 427 |
| CviQI | GTAC | 4 cut(s) | 216, 251, 278, 413 |
| DdeI | CTNAG | 2 cut(s) | 212, 294 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 396 |
| Eco47I | GGWCC | 1 cut(s) | 311 |
| Eco57I | CTGAAG | 3 cut(s) | 48, 135, 417 |
| EcoRII | CCWGG | 3 cut(s) | 218, 253, 383 |
| FaeI | CATG | 2 cut(s) | 145, 150 |
| FaiI | YATR | 4 cut(s) | 143, 148, 266, 323 |
| FaqI | GGGAC | 2 cut(s) | 324, 415 |
| FatI | CATG | 2 cut(s) | 141, 146 |
| FauI | CCCGC | 5 cut(s) | 8, 27, 55, 369, 429 |
| FblI | GTMKAC | 1 cut(s) | 229 |
| Fnu4HI | GCNGC | 1 cut(s) | 75 |
| FokI | GGATG | 2 cut(s) | 30, 38 |
| Fsp4HI | GCNGC | 1 cut(s) | 75 |
| GlaI | GCGC | 2 cut(s) | 101, 422 |
| GluI | GCNGC | 1 cut(s) | 75 |
| GsaI | CCCAGC | 3 cut(s) | 74, 194, 237 |
| GsuI | CTGGAG | 1 cut(s) | 194 |
| HaeIII | GGCC | 3 cut(s) | 179, 259, 383 |
| HapII | CCGG | 3 cut(s) | 240, 260, 433 |
| HhaI | GCGC | 2 cut(s) | 102, 423 |
| Hin1II | CATG | 2 cut(s) | 145, 150 |
| Hin6I | GCGC | 2 cut(s) | 100, 421 |
| HinP1I | GCGC | 2 cut(s) | 100, 421 |
| HincII | GTYRAC | 1 cut(s) | 230 |
| HindII | GTYRAC | 1 cut(s) | 230 |
| HinfI | GANTC | 1 cut(s) | 353 |
| HpaII | CCGG | 3 cut(s) | 240, 260, 433 |
| HphI | GGTGA | 1 cut(s) | 142 |
| Hpy166II | GTNNAC | 1 cut(s) | 230 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 379 |
| Hpy188III | TCNNGA | 1 cut(s) | 142 |
| Hpy8I | GTNNAC | 1 cut(s) | 230 |
| HpyAV | CCTTC | 1 cut(s) | 392 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 88 |
| HpyCH4V | TGCA | 1 cut(s) | 274 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 433 |
| HpyF3I | CTNAG | 2 cut(s) | 212, 294 |
| HpySE526I | ACGT | 1 cut(s) | 88 |
| Hsp92II | CATG | 2 cut(s) | 145, 150 |
| HspAI | GCGC | 2 cut(s) | 100, 421 |
| KpnI | GGTACC | 2 cut(s) | 254, 281 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 97, 340 |
| LweI | GCATC | 1 cut(s) | 261 |
| MaeII | ACGT | 1 cut(s) | 88 |
| MaeIII | GTNAC | 2 cut(s) | 130, 207 |
| MbiI | CCGCTC | 2 cut(s) | 34, 375 |
| MboII | GAAGA | 2 cut(s) | 41, 178 |
| MmeI | TCCRAC | 2 cut(s) | 90, 318 |
| MnlI | CCTC | 9 cut(s) | 73, 85, 88, 148, 169, 340, 373, 384, 411 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 3 cut(s) | 240, 260, 433 |
| MspR9I | CCNGG | 4 cut(s) | 220, 255, 385, 433 |
| MvaI | CCWGG | 3 cut(s) | 220, 255, 385 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 433 |
| NciI | CCSGG | 1 cut(s) | 433 |
| NlaIII | CATG | 2 cut(s) | 145, 150 |
| NlaIV | GGNNCC | 3 cut(s) | 252, 279, 312 |
| NmuCI | GTSAC | 2 cut(s) | 130, 207 |
| NspI | RCATGY | 1 cut(s) | 150 |
| PagI | TCATGA | 1 cut(s) | 141 |
| PciI | ACATGT | 1 cut(s) | 146 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 353 |
| PkrI | GCNGC | 1 cut(s) | 76 |
| PscI | ACATGT | 1 cut(s) | 146 |
| Psp6I | CCWGG | 3 cut(s) | 218, 253, 383 |
| PspFI | CCCAGC | 3 cut(s) | 70, 190, 233 |
| PspGI | CCWGG | 3 cut(s) | 218, 253, 383 |
| PspN4I | GGNNCC | 3 cut(s) | 252, 279, 312 |
| PspPI | GGNCC | 2 cut(s) | 257, 311 |
| RsaI | GTAC | 4 cut(s) | 217, 252, 279, 414 |
| RsaNI | GTAC | 4 cut(s) | 216, 251, 278, 413 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SalI | GTCGAC | 1 cut(s) | 228 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SatI | GCNGC | 1 cut(s) | 75 |
| Sau96I | GGNCC | 2 cut(s) | 257, 311 |
| ScrFI | CCNGG | 4 cut(s) | 220, 255, 385, 433 |
| SetI | ASST | 5 cut(s) | 91, 132, 279, 295, 403 |
| SfaNI | GCATC | 1 cut(s) | 261 |
| SinI | GGWCC | 1 cut(s) | 311 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SsiI | CCGC | 9 cut(s) | 15, 34, 48, 74, 187, 362, 373, 407, 436 |
| StyD4I | CCNGG | 4 cut(s) | 218, 253, 383, 431 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 91 |
| TaqI | TCGA | 1 cut(s) | 229 |
| TauI | GCSGC | 1 cut(s) | 77 |
| TfiI | GAWTC | 1 cut(s) | 353 |
| TscAI | CASTG | 2 cut(s) | 177, 288 |
| TseFI | GTSAC | 2 cut(s) | 130, 207 |
| Tsp45I | GTSAC | 2 cut(s) | 130, 207 |
| TspRI | CASTG | 2 cut(s) | 177, 288 |
| VpaK11BI | GGWCC | 1 cut(s) | 311 |
| XceI | RCATGY | 1 cut(s) | 150 |
| XmiI | GTMKAC | 1 cut(s) | 229 |