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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 172 |
| Acc36I | ACCTGC | 1 cut(s) | 172 |
| AciI | CCGC | 3 cut(s) | 93, 252, 302 |
| AcoI | YGGCCR | 2 cut(s) | 56, 186 |
| AcsI | RAATTY | 1 cut(s) | 203 |
| AdeI | CACNNNGTG | 1 cut(s) | 130 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 127, 181, 252 |
| AjnI | CCWGG | 4 cut(s) | 9, 76, 247, 275 |
| AluBI | AGCT | 3 cut(s) | 106, 142, 214 |
| AluI | AGCT | 3 cut(s) | 106, 142, 214 |
| Alw21I | GWGCWC | 1 cut(s) | 144 |
| AoxI | GGCC | 3 cut(s) | 56, 171, 186 |
| ApeKI | GCWGC | 1 cut(s) | 63 |
| ApoI | RAATTY | 1 cut(s) | 203 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 260 |
| AspLEI | GCGC | 2 cut(s) | 156, 226 |
| AspS9I | GGNCC | 2 cut(s) | 25, 172 |
| AsuHPI | GGTGA | 1 cut(s) | 142 |
| AvaII | GGWCC | 1 cut(s) | 25 |
| BalI | TGGCCA | 1 cut(s) | 188 |
| BanII | GRGCYC | 3 cut(s) | 17, 144, 287 |
| Bbv12I | GWGCWC | 1 cut(s) | 144 |
| BbvI | GCAGC | 1 cut(s) | 75 |
| BceAI | ACGGC | 1 cut(s) | 71 |
| BciT130I | CCWGG | 4 cut(s) | 11, 78, 249, 277 |
| BfmI | CTRYAG | 1 cut(s) | 42 |
| BfoI | RGCGCY | 1 cut(s) | 227 |
| BfuAI | ACCTGC | 1 cut(s) | 172 |
| BglII | AGATCT | 1 cut(s) | 85 |
| BisI | GCNGC | 2 cut(s) | 64, 93 |
| BlsI | GCNGC | 2 cut(s) | 65, 94 |
| Bme1390I | CCNGG | 4 cut(s) | 11, 78, 249, 277 |
| Bme18I | GGWCC | 1 cut(s) | 25 |
| BmgT120I | GGNCC | 2 cut(s) | 25, 172 |
| BmiI | GGNNCC | 2 cut(s) | 6, 27 |
| BmrFI | CCNGG | 4 cut(s) | 11, 78, 249, 277 |
| BpmI | CTGGAG | 2 cut(s) | 248, 259 |
| BsaJI | CCNNGG | 5 cut(s) | 9, 10, 21, 76, 247 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 127, 181, 252 |
| Bse1I | ACTGG | 1 cut(s) | 263 |
| BseBI | CCWGG | 4 cut(s) | 11, 78, 249, 277 |
| BseDI | CCNNGG | 5 cut(s) | 9, 10, 21, 76, 247 |
| BseGI | GGATG | 1 cut(s) | 17 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 127, 181, 252 |
| BseNI | ACTGG | 1 cut(s) | 263 |
| BseXI | GCAGC | 1 cut(s) | 75 |
| BshFI | GGCC | 3 cut(s) | 58, 173, 188 |
| BsiHKAI | GWGCWC | 1 cut(s) | 144 |
| BslFI | GGGAC | 2 cut(s) | 11, 229 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 127, 181, 252 |
| BsmFI | GGGAC | 2 cut(s) | 11, 229 |
| BsnI | GGCC | 3 cut(s) | 58, 173, 188 |
| Bsp1286I | GDGCHC | 3 cut(s) | 17, 144, 287 |
| Bsp143I | GATC | 1 cut(s) | 85 |
| BspACI | CCGC | 3 cut(s) | 93, 252, 302 |
| BspANI | GGCC | 3 cut(s) | 58, 173, 188 |
| BspLI | GGNNCC | 2 cut(s) | 6, 27 |
| BspMAI | CTGCAG | 1 cut(s) | 46 |
| BspMI | ACCTGC | 1 cut(s) | 172 |
| BsrI | ACTGG | 1 cut(s) | 263 |
| BssECI | CCNNGG | 5 cut(s) | 9, 10, 21, 76, 247 |
| BssMI | GATC | 1 cut(s) | 85 |
| Bst2UI | CCWGG | 4 cut(s) | 11, 78, 249, 277 |
| BstC8I | GCNNGC | 3 cut(s) | 108, 287, 291 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 125 |
| BstF5I | GGATG | 1 cut(s) | 17 |
| BstH2I | RGCGCY | 1 cut(s) | 227 |
| BstHHI | GCGC | 2 cut(s) | 156, 226 |
| BstKTI | GATC | 1 cut(s) | 88 |
| BstMBI | GATC | 1 cut(s) | 85 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 50, 69, 230, 286 |
| BstNI | CCWGG | 4 cut(s) | 11, 78, 249, 277 |
| BstSCI | CCNGG | 4 cut(s) | 9, 76, 247, 275 |
| BstSFI | CTRYAG | 1 cut(s) | 42 |
| BstV1I | GCAGC | 1 cut(s) | 75 |
| BstX2I | RGATCY | 1 cut(s) | 85 |
| BstYI | RGATCY | 1 cut(s) | 85 |
| BsuRI | GGCC | 3 cut(s) | 58, 173, 188 |
| BtsCI | GGATG | 1 cut(s) | 17 |
| BtsIMutI | CAGTG | 1 cut(s) | 29 |
| BveI | ACCTGC | 1 cut(s) | 172 |
| Cac8I | GCNNGC | 3 cut(s) | 108, 287, 291 |
| CfoI | GCGC | 2 cut(s) | 156, 226 |
| Cfr13I | GGNCC | 2 cut(s) | 25, 172 |
| CviJI | RGCY | 8 cut(s) | 15, 58, 106, 142, 173, 188, 214, 285 |
| CviKI_1 | RGCY | 8 cut(s) | 15, 58, 106, 142, 173, 188, 214, 285 |
| DpnI | GATC | 1 cut(s) | 87 |
| DpnII | GATC | 1 cut(s) | 85 |
| DraIII | CACNNNGTG | 1 cut(s) | 130 |
| EaeI | YGGCCR | 2 cut(s) | 56, 186 |
| Ecl136II | GAGCTC | 1 cut(s) | 142 |
| Eco24I | GRGCYC | 3 cut(s) | 17, 144, 287 |
| Eco47I | GGWCC | 1 cut(s) | 25 |
| Eco53kI | GAGCTC | 1 cut(s) | 142 |
| EcoICRI | GAGCTC | 1 cut(s) | 142 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 125 |
| EcoO109I | RGGNCCY | 1 cut(s) | 25 |
| EcoRII | CCWGG | 4 cut(s) | 9, 76, 247, 275 |
| EcoT38I | GRGCYC | 3 cut(s) | 17, 144, 287 |
| FaiI | YATR | 2 cut(s) | 98, 102 |
| FaqI | GGGAC | 2 cut(s) | 11, 229 |
| FauI | CCCGC | 1 cut(s) | 245 |
| Fnu4HI | GCNGC | 2 cut(s) | 64, 93 |
| FokI | GGATG | 1 cut(s) | 4 |
| FriOI | GRGCYC | 3 cut(s) | 17, 144, 287 |
| Fsp4HI | GCNGC | 2 cut(s) | 64, 93 |
| GlaI | GCGC | 2 cut(s) | 155, 225 |
| GluI | GCNGC | 2 cut(s) | 64, 93 |
| GsuI | CTGGAG | 2 cut(s) | 248, 259 |
| HaeII | RGCGCY | 1 cut(s) | 227 |
| HaeIII | GGCC | 3 cut(s) | 58, 173, 188 |
| HhaI | GCGC | 2 cut(s) | 156, 226 |
| Hin6I | GCGC | 2 cut(s) | 154, 224 |
| HinP1I | GCGC | 2 cut(s) | 154, 224 |
| HphI | GGTGA | 1 cut(s) | 142 |
| Hpy188I | TCNGA | 1 cut(s) | 22 |
| Hpy188III | TCNNGA | 1 cut(s) | 83 |
| Hpy99I | CGWCG | 1 cut(s) | 212 |
| HpyAV | CCTTC | 1 cut(s) | 157 |
| HpyCH4V | TGCA | 1 cut(s) | 44 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 50, 69, 230, 286 |
| HspAI | GCGC | 2 cut(s) | 154, 224 |
| Kzo9I | GATC | 1 cut(s) | 85 |
| LmnI | GCTCC | 1 cut(s) | 139 |
| Lsp1109I | GCAGC | 1 cut(s) | 75 |
| MalI | GATC | 1 cut(s) | 87 |
| MboI | GATC | 1 cut(s) | 85 |
| MflI | RGATCY | 1 cut(s) | 85 |
| MhlI | GDGCHC | 3 cut(s) | 17, 144, 287 |
| MlsI | TGGCCA | 1 cut(s) | 188 |
| MluCI | AATT | 1 cut(s) | 203 |
| MluNI | TGGCCA | 1 cut(s) | 188 |
| MnlI | CCTC | 5 cut(s) | 16, 43, 131, 163, 223 |
| Mox20I | TGGCCA | 1 cut(s) | 188 |
| MroXI | GAANNNNTTC | 1 cut(s) | 260 |
| MscI | TGGCCA | 1 cut(s) | 188 |
| MseI | TTAA | 1 cut(s) | 307 |
| MslI | CAYNNNNRTG | 1 cut(s) | 194 |
| Msp20I | TGGCCA | 1 cut(s) | 188 |
| MspR9I | CCNGG | 4 cut(s) | 11, 78, 249, 277 |
| MvaI | CCWGG | 4 cut(s) | 11, 78, 249, 277 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 50, 69, 230, 286 |
| NdeII | GATC | 1 cut(s) | 85 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 27 |
| PaqCI | CACCTGC | 1 cut(s) | 172 |
| PasI | CCCWGGG | 1 cut(s) | 10 |
| PdmI | GAANNNNTTC | 1 cut(s) | 260 |
| PkrI | GCNGC | 2 cut(s) | 65, 94 |
| PpuMI | RGGWCCY | 1 cut(s) | 25 |
| Psp124BI | GAGCTC | 1 cut(s) | 144 |
| Psp5II | RGGWCCY | 1 cut(s) | 25 |
| Psp6I | CCWGG | 4 cut(s) | 9, 76, 247, 275 |
| PspGI | CCWGG | 4 cut(s) | 9, 76, 247, 275 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 27 |
| PspPI | GGNCC | 2 cut(s) | 25, 172 |
| PspPPI | RGGWCCY | 1 cut(s) | 25 |
| PstI | CTGCAG | 1 cut(s) | 46 |
| PsuI | RGATCY | 1 cut(s) | 85 |
| RseI | CAYNNNNRTG | 1 cut(s) | 194 |
| SacI | GAGCTC | 1 cut(s) | 144 |
| SaqAI | TTAA | 1 cut(s) | 307 |
| SatI | GCNGC | 2 cut(s) | 64, 93 |
| Sau3AI | GATC | 1 cut(s) | 85 |
| Sau96I | GGNCC | 2 cut(s) | 25, 172 |
| ScrFI | CCNGG | 4 cut(s) | 11, 78, 249, 277 |
| SduI | GDGCHC | 3 cut(s) | 17, 144, 287 |
| SetI | ASST | 9 cut(s) | 27, 82, 108, 123, 132, 144, 168, 186, 216 |
| SfcI | CTRYAG | 1 cut(s) | 42 |
| SinI | GGWCC | 1 cut(s) | 25 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 194 |
| Sse9I | AATT | 1 cut(s) | 203 |
| SsiI | CCGC | 3 cut(s) | 93, 252, 302 |
| SstI | GAGCTC | 1 cut(s) | 144 |
| StyD4I | CCNGG | 4 cut(s) | 9, 76, 247, 275 |
| TaqI | TCGA | 3 cut(s) | 144, 168, 207 |
| TasI | AATT | 1 cut(s) | 203 |
| TauI | GCSGC | 1 cut(s) | 95 |
| Tru1I | TTAA | 1 cut(s) | 307 |
| Tru9I | TTAA | 1 cut(s) | 307 |
| TscAI | CASTG | 1 cut(s) | 36 |
| TseI | GCWGC | 1 cut(s) | 63 |
| TspRI | CASTG | 1 cut(s) | 36 |
| VpaK11BI | GGWCC | 1 cut(s) | 25 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 125 |
| XapI | RAATTY | 1 cut(s) | 203 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 255 |
| XmnI | GAANNNNTTC | 1 cut(s) | 260 |