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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 414 |
| AccII | CGCG | 1 cut(s) | 69 |
| AciI | CCGC | 6 cut(s) | 67, 72, 143, 156, 215, 342 |
| AclWI | GGATC | 2 cut(s) | 280, 293 |
| AcyI | GRCGYC | 1 cut(s) | 411 |
| AdeI | CACNNNGTG | 1 cut(s) | 420 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 78, 114, 115, 142, 155, 175, 338 |
| AgsI | TTSAA | 1 cut(s) | 379 |
| AjnI | CCWGG | 5 cut(s) | 77, 138, 174, 260, 281 |
| AluBI | AGCT | 2 cut(s) | 25, 205 |
| AluI | AGCT | 2 cut(s) | 25, 205 |
| AlwI | GGATC | 2 cut(s) | 280, 293 |
| AoxI | GGCC | 3 cut(s) | 146, 225, 352 |
| ApaI | GGGCCC | 2 cut(s) | 150, 229 |
| AspLEI | GCGC | 1 cut(s) | 71 |
| AspS9I | GGNCC | 5 cut(s) | 146, 147, 225, 226, 353 |
| AsuHPI | GGTGA | 2 cut(s) | 268, 406 |
| BaeGI | GKGCMC | 2 cut(s) | 150, 229 |
| BamHI | GGATCC | 1 cut(s) | 285 |
| BanII | GRGCYC | 2 cut(s) | 150, 229 |
| BbvCI | CCTCAGC | 1 cut(s) | 21 |
| BceAI | ACGGC | 2 cut(s) | 281, 355 |
| BciT130I | CCWGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| BfaI | CTAG | 1 cut(s) | 84 |
| BisI | GCNGC | 2 cut(s) | 72, 343 |
| BlsI | GCNGC | 2 cut(s) | 73, 344 |
| Bme1390I | CCNGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| BmgT120I | GGNCC | 5 cut(s) | 146, 147, 225, 226, 353 |
| BmiI | GGNNCC | 8 cut(s) | 120, 134, 147, 148, 227, 228, 287, 355 |
| BmrFI | CCNGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| BmsI | GCATC | 1 cut(s) | 174 |
| BpmI | CTGGAG | 2 cut(s) | 53, 122 |
| Bpu10I | CCTNAGC | 1 cut(s) | 21 |
| BsaHI | GRCGYC | 1 cut(s) | 411 |
| BsaJI | CCNNGG | 1 cut(s) | 78 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 32, 62 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 78, 114, 115, 142, 155, 175, 338 |
| Bse118I | RCCGGY | 1 cut(s) | 121 |
| Bse1I | ACTGG | 1 cut(s) | 355 |
| Bse3DI | GCAATG | 1 cut(s) | 134 |
| BseBI | CCWGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| BseDI | CCNNGG | 1 cut(s) | 78 |
| BseGI | GGATG | 1 cut(s) | 189 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 78, 114, 115, 142, 155, 175, 338 |
| BseMI | GCAATG | 1 cut(s) | 134 |
| BseMII | CTCAG | 2 cut(s) | 35, 81 |
| BseNI | ACTGG | 1 cut(s) | 355 |
| BseSI | GKGCMC | 2 cut(s) | 150, 229 |
| Bsh1236I | CGCG | 1 cut(s) | 69 |
| BshFI | GGCC | 3 cut(s) | 148, 227, 354 |
| BsiSI | CCGG | 1 cut(s) | 122 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 78, 114, 115, 142, 155, 175, 338 |
| BsnI | GGCC | 3 cut(s) | 148, 227, 354 |
| Bsp120I | GGGCCC | 2 cut(s) | 146, 225 |
| Bsp1286I | GDGCHC | 2 cut(s) | 150, 229 |
| Bsp143I | GATC | 1 cut(s) | 285 |
| BspACI | CCGC | 6 cut(s) | 67, 72, 143, 156, 215, 342 |
| BspANI | GGCC | 3 cut(s) | 148, 227, 354 |
| BspCNI | CTCAG | 2 cut(s) | 34, 82 |
| BspFNI | CGCG | 1 cut(s) | 69 |
| BspLI | GGNNCC | 8 cut(s) | 120, 134, 147, 148, 227, 228, 287, 355 |
| BspPI | GGATC | 2 cut(s) | 280, 293 |
| BsrDI | GCAATG | 1 cut(s) | 134 |
| BsrFI | RCCGGY | 1 cut(s) | 121 |
| BsrI | ACTGG | 1 cut(s) | 355 |
| BssAI | RCCGGY | 1 cut(s) | 121 |
| BssECI | CCNNGG | 1 cut(s) | 78 |
| BssMI | GATC | 1 cut(s) | 285 |
| BssNI | GRCGYC | 1 cut(s) | 411 |
| Bst2UI | CCWGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| BstACI | GRCGYC | 1 cut(s) | 411 |
| BstC8I | GCNNGC | 1 cut(s) | 294 |
| BstDEI | CTNAG | 2 cut(s) | 21, 90 |
| BstF5I | GGATG | 1 cut(s) | 189 |
| BstFNI | CGCG | 1 cut(s) | 69 |
| BstHHI | GCGC | 1 cut(s) | 71 |
| BstKTI | GATC | 1 cut(s) | 288 |
| BstMBI | GATC | 1 cut(s) | 285 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 66, 80, 351 |
| BstNI | CCWGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| BstNSI | RCATGY | 1 cut(s) | 314 |
| BstSCI | CCNGG | 5 cut(s) | 77, 138, 174, 260, 281 |
| BstSLI | GKGCMC | 2 cut(s) | 150, 229 |
| BstUI | CGCG | 1 cut(s) | 69 |
| BstX2I | RGATCY | 1 cut(s) | 285 |
| BstYI | RGATCY | 1 cut(s) | 285 |
| BsuRI | GGCC | 3 cut(s) | 148, 227, 354 |
| BtsCI | GGATG | 1 cut(s) | 189 |
| Cac8I | GCNNGC | 1 cut(s) | 294 |
| CfoI | GCGC | 1 cut(s) | 71 |
| Cfr10I | RCCGGY | 1 cut(s) | 121 |
| Cfr13I | GGNCC | 5 cut(s) | 146, 147, 225, 226, 353 |
| CseI | GACGC | 1 cut(s) | 246 |
| CviAII | CATG | 3 cut(s) | 311, 346, 427 |
| CviJI | RGCY | 9 cut(s) | 25, 83, 121, 148, 205, 227, 296, 331, 354 |
| CviKI_1 | RGCY | 9 cut(s) | 25, 83, 121, 148, 205, 227, 296, 331, 354 |
| DdeI | CTNAG | 2 cut(s) | 21, 90 |
| DpnI | GATC | 1 cut(s) | 287 |
| DpnII | GATC | 1 cut(s) | 285 |
| DraIII | CACNNNGTG | 1 cut(s) | 420 |
| EciI | GGCGGA | 1 cut(s) | 230 |
| Eco24I | GRGCYC | 2 cut(s) | 150, 229 |
| EcoO109I | RGGNCCY | 3 cut(s) | 146, 225, 226 |
| EcoRII | CCWGG | 5 cut(s) | 77, 138, 174, 260, 281 |
| EcoT38I | GRGCYC | 2 cut(s) | 150, 229 |
| FaeI | CATG | 3 cut(s) | 314, 349, 430 |
| FaiI | YATR | 5 cut(s) | 312, 347, 366, 368, 428 |
| FatI | CATG | 3 cut(s) | 310, 345, 426 |
| FauI | CCCGC | 1 cut(s) | 136 |
| FauNDI | CATATG | 1 cut(s) | 366 |
| Fnu4HI | GCNGC | 2 cut(s) | 72, 343 |
| FokI | GGATG | 1 cut(s) | 196 |
| FriOI | GRGCYC | 2 cut(s) | 150, 229 |
| Fsp4HI | GCNGC | 2 cut(s) | 72, 343 |
| FspBI | CTAG | 1 cut(s) | 84 |
| GlaI | GCGC | 1 cut(s) | 70 |
| GluI | GCNGC | 2 cut(s) | 72, 343 |
| GsuI | CTGGAG | 2 cut(s) | 53, 122 |
| HaeIII | GGCC | 3 cut(s) | 148, 227, 354 |
| HapII | CCGG | 1 cut(s) | 122 |
| HgaI | GACGC | 1 cut(s) | 246 |
| HhaI | GCGC | 1 cut(s) | 71 |
| Hin1I | GRCGYC | 1 cut(s) | 411 |
| Hin1II | CATG | 3 cut(s) | 314, 349, 430 |
| Hin6I | GCGC | 1 cut(s) | 69 |
| HinP1I | GCGC | 1 cut(s) | 69 |
| HpaII | CCGG | 1 cut(s) | 122 |
| HphI | GGTGA | 2 cut(s) | 268, 406 |
| Hpy188I | TCNGA | 1 cut(s) | 302 |
| Hpy188III | TCNNGA | 1 cut(s) | 267 |
| HpyAV | CCTTC | 3 cut(s) | 21, 367, 385 |
| HpyCH4IV | ACGT | 1 cut(s) | 411 |
| HpyCH4V | TGCA | 5 cut(s) | 127, 161, 187, 292, 314 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 66, 80, 351 |
| HpyF3I | CTNAG | 2 cut(s) | 21, 90 |
| HpySE526I | ACGT | 1 cut(s) | 411 |
| Hsp92I | GRCGYC | 1 cut(s) | 411 |
| Hsp92II | CATG | 3 cut(s) | 314, 349, 430 |
| HspAI | GCGC | 1 cut(s) | 69 |
| Kzo9I | GATC | 1 cut(s) | 285 |
| LmnI | GCTCC | 3 cut(s) | 34, 118, 423 |
| LweI | GCATC | 1 cut(s) | 174 |
| MaeI | CTAG | 1 cut(s) | 84 |
| MaeII | ACGT | 1 cut(s) | 411 |
| MaeIII | GTNAC | 2 cut(s) | 385, 412 |
| MalI | GATC | 1 cut(s) | 287 |
| MboI | GATC | 1 cut(s) | 285 |
| MflI | RGATCY | 1 cut(s) | 285 |
| MhlI | GDGCHC | 2 cut(s) | 150, 229 |
| MluCI | AATT | 3 cut(s) | 191, 207, 237 |
| MnlI | CCTC | 7 cut(s) | 30, 34, 85, 108, 146, 211, 258 |
| MspI | CCGG | 1 cut(s) | 122 |
| MspR9I | CCNGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| MvaI | CCWGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| MvnI | CGCG | 1 cut(s) | 69 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 66, 80, 351 |
| NdeI | CATATG | 1 cut(s) | 366 |
| NdeII | GATC | 1 cut(s) | 285 |
| NlaIII | CATG | 3 cut(s) | 314, 349, 430 |
| NlaIV | GGNNCC | 8 cut(s) | 120, 134, 147, 148, 227, 228, 287, 355 |
| NmuCI | GTSAC | 2 cut(s) | 385, 412 |
| NspI | RCATGY | 1 cut(s) | 314 |
| PfoI | TCCNGGA | 1 cut(s) | 174 |
| PkrI | GCNGC | 2 cut(s) | 73, 344 |
| Psp6I | CCWGG | 5 cut(s) | 77, 138, 174, 260, 281 |
| PspGI | CCWGG | 5 cut(s) | 77, 138, 174, 260, 281 |
| PspN4I | GGNNCC | 8 cut(s) | 120, 134, 147, 148, 227, 228, 287, 355 |
| PspOMI | GGGCCC | 2 cut(s) | 146, 225 |
| PspPI | GGNCC | 5 cut(s) | 146, 147, 225, 226, 353 |
| PsuI | RGATCY | 1 cut(s) | 285 |
| SatI | GCNGC | 2 cut(s) | 72, 343 |
| Sau3AI | GATC | 1 cut(s) | 285 |
| Sau96I | GGNCC | 5 cut(s) | 146, 147, 225, 226, 353 |
| ScrFI | CCNGG | 5 cut(s) | 79, 140, 176, 262, 283 |
| SduI | GDGCHC | 2 cut(s) | 150, 229 |
| SetI | ASST | 7 cut(s) | 13, 27, 138, 207, 266, 377, 414 |
| SfaNI | GCATC | 1 cut(s) | 174 |
| Sse9I | AATT | 3 cut(s) | 191, 207, 237 |
| SsiI | CCGC | 6 cut(s) | 67, 72, 143, 156, 215, 342 |
| SspMI | CTAG | 1 cut(s) | 84 |
| StyD4I | CCNGG | 5 cut(s) | 77, 138, 174, 260, 281 |
| TaiI | ACGT | 1 cut(s) | 414 |
| TasI | AATT | 3 cut(s) | 191, 207, 237 |
| TauI | GCSGC | 2 cut(s) | 74, 345 |
| TseFI | GTSAC | 2 cut(s) | 385, 412 |
| Tsp45I | GTSAC | 2 cut(s) | 385, 412 |
| XceI | RCATGY | 1 cut(s) | 314 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 172 |
| XspI | CTAG | 1 cut(s) | 84 |
| ZraI | GACGTC | 1 cut(s) | 412 |