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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 115 |
| AccB1I | GGYRCC | 1 cut(s) | 516 |
| AccBSI | CCGCTC | 2 cut(s) | 169, 542 |
| AccII | CGCG | 4 cut(s) | 108, 435, 448, 453 |
| AciI | CCGC | 9 cut(s) | 15, 48, 106, 167, 231, 433, 435, 507, 540 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AcuI | CTGAAG | 1 cut(s) | 374 |
| AcyI | GRCGYC | 1 cut(s) | 517 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 139, 265, 439, 467 |
| AgsI | TTSAA | 2 cut(s) | 118, 535 |
| AjnI | CCWGG | 4 cut(s) | 147, 159, 196, 214 |
| Alw26I | GTCTC | 2 cut(s) | 68, 437 |
| AoxI | GGCC | 4 cut(s) | 217, 303, 309, 324 |
| ApeKI | GCWGC | 1 cut(s) | 182 |
| AspLEI | GCGC | 5 cut(s) | 110, 294, 364, 388, 519 |
| AspS9I | GGNCC | 2 cut(s) | 163, 310 |
| AvaII | GGWCC | 1 cut(s) | 163 |
| BaeGI | GKGCMC | 1 cut(s) | 155 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanI | GGYRCC | 1 cut(s) | 516 |
| BbvI | GCAGC | 1 cut(s) | 194 |
| BccI | CCATC | 1 cut(s) | 66 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 438, 472 |
| BciT130I | CCWGG | 4 cut(s) | 149, 161, 198, 216 |
| BcoDI | GTCTC | 2 cut(s) | 68, 437 |
| BfaI | CTAG | 2 cut(s) | 135, 503 |
| BfmI | CTRYAG | 1 cut(s) | 180 |
| BfoI | RGCGCY | 2 cut(s) | 365, 520 |
| BfuAI | ACCTGC | 1 cut(s) | 115 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 216 |
| BisI | GCNGC | 2 cut(s) | 183, 231 |
| BlsI | GCNGC | 2 cut(s) | 184, 232 |
| Bme1390I | CCNGG | 4 cut(s) | 149, 161, 198, 216 |
| Bme18I | GGWCC | 1 cut(s) | 163 |
| BmgT120I | GGNCC | 2 cut(s) | 163, 310 |
| BmiI | GGNNCC | 3 cut(s) | 144, 165, 518 |
| BmrFI | CCNGG | 4 cut(s) | 149, 161, 198, 216 |
| BmsI | GCATC | 1 cut(s) | 466 |
| BpmI | CTGGAG | 1 cut(s) | 386 |
| BsaHI | GRCGYC | 1 cut(s) | 517 |
| BsaJI | CCNNGG | 5 cut(s) | 147, 148, 159, 306, 433 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 139, 265, 439, 467 |
| BseBI | CCWGG | 4 cut(s) | 149, 161, 198, 216 |
| BseDI | CCNNGG | 5 cut(s) | 147, 148, 159, 306, 433 |
| BseGI | GGATG | 2 cut(s) | 58, 481 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 139, 265, 439, 467 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseSI | GKGCMC | 1 cut(s) | 155 |
| BseXI | GCAGC | 1 cut(s) | 194 |
| BseYI | CCCAGC | 3 cut(s) | 26, 382, 521 |
| Bsh1236I | CGCG | 4 cut(s) | 108, 435, 448, 453 |
| BshFI | GGCC | 4 cut(s) | 219, 305, 311, 326 |
| BshNI | GGYRCC | 1 cut(s) | 516 |
| BslFI | GGGAC | 1 cut(s) | 149 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 139, 265, 439, 467 |
| BsmAI | GTCTC | 2 cut(s) | 68, 437 |
| BsmBI | CGTCTC | 1 cut(s) | 437 |
| BsmFI | GGGAC | 1 cut(s) | 149 |
| BsnI | GGCC | 4 cut(s) | 219, 305, 311, 326 |
| Bsp1286I | GDGCHC | 1 cut(s) | 155 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 9 cut(s) | 15, 48, 106, 167, 231, 433, 435, 507, 540 |
| BspANI | GGCC | 4 cut(s) | 219, 305, 311, 326 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspFNI | CGCG | 4 cut(s) | 108, 435, 448, 453 |
| BspLI | GGNNCC | 3 cut(s) | 144, 165, 518 |
| BspMAI | CTGCAG | 1 cut(s) | 184 |
| BspMI | ACCTGC | 1 cut(s) | 115 |
| BspT107I | GGYRCC | 1 cut(s) | 516 |
| BsrBI | CCGCTC | 2 cut(s) | 169, 542 |
| BssECI | CCNNGG | 5 cut(s) | 147, 148, 159, 306, 433 |
| BssMI | GATC | 1 cut(s) | 40 |
| BssNI | GRCGYC | 1 cut(s) | 517 |
| Bst2UI | CCWGG | 4 cut(s) | 149, 161, 198, 216 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 94 |
| Bst6I | CTCTTC | 1 cut(s) | 350 |
| BstACI | GRCGYC | 1 cut(s) | 517 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 284, 395, 515 |
| BstDEI | CTNAG | 1 cut(s) | 72 |
| BstDSI | CCRYGG | 1 cut(s) | 433 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 2 cut(s) | 58, 481 |
| BstFNI | CGCG | 4 cut(s) | 108, 435, 448, 453 |
| BstH2I | RGCGCY | 2 cut(s) | 365, 520 |
| BstHHI | GCGC | 5 cut(s) | 110, 294, 364, 388, 519 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMAI | GTCTC | 2 cut(s) | 68, 437 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 188, 216, 383, 394 |
| BstNI | CCWGG | 4 cut(s) | 149, 161, 198, 216 |
| BstSCI | CCNGG | 4 cut(s) | 147, 159, 196, 214 |
| BstSFI | CTRYAG | 1 cut(s) | 180 |
| BstSLI | GKGCMC | 1 cut(s) | 155 |
| BstUI | CGCG | 4 cut(s) | 108, 435, 448, 453 |
| BstV1I | GCAGC | 1 cut(s) | 194 |
| BsuRI | GGCC | 4 cut(s) | 219, 305, 311, 326 |
| BtgI | CCRYGG | 1 cut(s) | 433 |
| BtsCI | GGATG | 2 cut(s) | 58, 481 |
| BveI | ACCTGC | 1 cut(s) | 115 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 284, 395, 515 |
| CfoI | GCGC | 5 cut(s) | 110, 294, 364, 388, 519 |
| Cfr13I | GGNCC | 2 cut(s) | 163, 310 |
| Cfr42I | CCGCGG | 1 cut(s) | 436 |
| CseI | GACGC | 3 cut(s) | 454, 459, 538 |
| CsiI | ACCWGGT | 1 cut(s) | 196 |
| CviAII | CATG | 1 cut(s) | 328 |
| CviJI | RGCY | 8 cut(s) | 26, 191, 219, 254, 305, 311, 326, 497 |
| CviKI_1 | RGCY | 8 cut(s) | 26, 191, 219, 254, 305, 311, 326, 497 |
| DdeI | CTNAG | 1 cut(s) | 72 |
| DinI | GGCGCC | 1 cut(s) | 518 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| Eam1104I | CTCTTC | 1 cut(s) | 350 |
| EarI | CTCTTC | 1 cut(s) | 350 |
| EciI | GGCGGA | 2 cut(s) | 37, 522 |
| Eco32I | GATATC | 1 cut(s) | 336 |
| Eco47I | GGWCC | 1 cut(s) | 163 |
| Eco57I | CTGAAG | 1 cut(s) | 374 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoRII | CCWGG | 4 cut(s) | 147, 159, 196, 214 |
| EcoRV | GATATC | 1 cut(s) | 336 |
| EgeI | GGCGCC | 1 cut(s) | 518 |
| EheI | GGCGCC | 1 cut(s) | 518 |
| Esp3I | CGTCTC | 1 cut(s) | 437 |
| FaeI | CATG | 1 cut(s) | 331 |
| FaiI | YATR | 3 cut(s) | 236, 240, 329 |
| FaqI | GGGAC | 1 cut(s) | 149 |
| FatI | CATG | 1 cut(s) | 327 |
| FauI | CCCGC | 3 cut(s) | 8, 174, 440 |
| Fnu4HI | GCNGC | 2 cut(s) | 183, 231 |
| FokI | GGATG | 2 cut(s) | 45, 488 |
| Fsp4HI | GCNGC | 2 cut(s) | 183, 231 |
| FspBI | CTAG | 2 cut(s) | 135, 503 |
| GlaI | GCGC | 5 cut(s) | 109, 293, 363, 387, 518 |
| GluI | GCNGC | 2 cut(s) | 183, 231 |
| GsaI | CCCAGC | 3 cut(s) | 30, 386, 525 |
| GsuI | CTGGAG | 1 cut(s) | 386 |
| HaeII | RGCGCY | 2 cut(s) | 365, 520 |
| HaeIII | GGCC | 4 cut(s) | 219, 305, 311, 326 |
| HgaI | GACGC | 3 cut(s) | 454, 459, 538 |
| HhaI | GCGC | 5 cut(s) | 110, 294, 364, 388, 519 |
| Hin1I | GRCGYC | 1 cut(s) | 517 |
| Hin1II | CATG | 1 cut(s) | 331 |
| Hin6I | GCGC | 5 cut(s) | 108, 292, 362, 386, 517 |
| HinP1I | GCGC | 5 cut(s) | 108, 292, 362, 386, 517 |
| HincII | GTYRAC | 4 cut(s) | 85, 103, 130, 422 |
| HindII | GTYRAC | 4 cut(s) | 85, 103, 130, 422 |
| HinfI | GANTC | 1 cut(s) | 342 |
| HpaI | GTTAAC | 2 cut(s) | 85, 103 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 103, 130, 422 |
| Hpy188III | TCNNGA | 1 cut(s) | 339 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 103, 130, 422 |
| Hpy99I | CGWCG | 1 cut(s) | 453 |
| HpyAV | CCTTC | 2 cut(s) | 269, 295 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 94 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 415 |
| HpyCH4V | TGCA | 2 cut(s) | 182, 479 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 188, 216, 383, 394 |
| HpyF3I | CTNAG | 1 cut(s) | 72 |
| HpySE526I | ACGT | 2 cut(s) | 81, 415 |
| Hsp92I | GRCGYC | 1 cut(s) | 517 |
| Hsp92II | CATG | 1 cut(s) | 331 |
| HspAI | GCGC | 5 cut(s) | 108, 292, 362, 386, 517 |
| KasI | GGCGCC | 1 cut(s) | 516 |
| KspAI | GTTAAC | 2 cut(s) | 85, 103 |
| KspI | CCGCGG | 1 cut(s) | 436 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LmnI | GCTCC | 2 cut(s) | 31, 289 |
| Lsp1109I | GCAGC | 1 cut(s) | 194 |
| LweI | GCATC | 1 cut(s) | 466 |
| MabI | ACCWGGT | 1 cut(s) | 196 |
| MaeI | CTAG | 2 cut(s) | 135, 503 |
| MaeII | ACGT | 2 cut(s) | 81, 415 |
| MalI | GATC | 1 cut(s) | 42 |
| MbiI | CCGCTC | 2 cut(s) | 169, 542 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 1 cut(s) | 367 |
| MhlI | GDGCHC | 1 cut(s) | 155 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluCI | AATT | 2 cut(s) | 483, 529 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| Mly113I | GGCGCC | 1 cut(s) | 517 |
| MnlI | CCTC | 5 cut(s) | 181, 301, 351, 361, 503 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 435 |
| MspR9I | CCNGG | 4 cut(s) | 149, 161, 198, 216 |
| MvaI | CCWGG | 4 cut(s) | 149, 161, 198, 216 |
| MvnI | CGCG | 4 cut(s) | 108, 435, 448, 453 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 188, 216, 383, 394 |
| NarI | GGCGCC | 1 cut(s) | 517 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIII | CATG | 1 cut(s) | 331 |
| NlaIV | GGNNCC | 3 cut(s) | 144, 165, 518 |
| NmeAIII | GCCGAG | 1 cut(s) | 331 |
| PasI | CCCWGGG | 1 cut(s) | 148 |
| PfeI | GAWTC | 1 cut(s) | 342 |
| PflFI | GACNNNGTC | 1 cut(s) | 417 |
| PkrI | GCNGC | 2 cut(s) | 184, 232 |
| PluTI | GGCGCC | 1 cut(s) | 520 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp6I | CCWGG | 4 cut(s) | 147, 159, 196, 214 |
| PspFI | CCCAGC | 3 cut(s) | 26, 382, 521 |
| PspGI | CCWGG | 4 cut(s) | 147, 159, 196, 214 |
| PspN4I | GGNNCC | 3 cut(s) | 144, 165, 518 |
| PspPI | GGNCC | 2 cut(s) | 163, 310 |
| PstI | CTGCAG | 1 cut(s) | 184 |
| PsyI | GACNNNGTC | 1 cut(s) | 417 |
| SacII | CCGCGG | 1 cut(s) | 436 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 2 cut(s) | 183, 231 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 2 cut(s) | 163, 310 |
| ScrFI | CCNGG | 4 cut(s) | 149, 161, 198, 216 |
| SduI | GDGCHC | 1 cut(s) | 155 |
| SetI | ASST | 6 cut(s) | 84, 129, 202, 228, 261, 418 |
| SexAI | ACCWGGT | 1 cut(s) | 196 |
| SfaNI | GCATC | 1 cut(s) | 466 |
| SfcI | CTRYAG | 1 cut(s) | 180 |
| SfoI | GGCGCC | 1 cut(s) | 518 |
| Sfr303I | CCGCGG | 1 cut(s) | 436 |
| SgrBI | CCGCGG | 1 cut(s) | 436 |
| SinI | GGWCC | 1 cut(s) | 163 |
| Sse9I | AATT | 2 cut(s) | 483, 529 |
| SsiI | CCGC | 9 cut(s) | 15, 48, 106, 167, 231, 433, 435, 507, 540 |
| SspDI | GGCGCC | 1 cut(s) | 516 |
| SspMI | CTAG | 2 cut(s) | 135, 503 |
| StyD4I | CCNGG | 4 cut(s) | 147, 159, 196, 214 |
| TaaI | ACNGT | 2 cut(s) | 11, 94 |
| TaiI | ACGT | 2 cut(s) | 84, 418 |
| TaqI | TCGA | 2 cut(s) | 193, 345 |
| TasI | AATT | 2 cut(s) | 483, 529 |
| TauI | GCSGC | 1 cut(s) | 233 |
| TfiI | GAWTC | 1 cut(s) | 342 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TseI | GCWGC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 1 cut(s) | 363 |
| Tth111I | GACNNNGTC | 1 cut(s) | 417 |
| VpaK11BI | GGWCC | 1 cut(s) | 163 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XspI | CTAG | 2 cut(s) | 135, 503 |