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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 271 |
| Acc36I | ACCTGC | 2 cut(s) | 124, 271 |
| Acc65I | GGTACC | 2 cut(s) | 109, 136 |
| AccB1I | GGYRCC | 2 cut(s) | 109, 136 |
| AccBSI | CCGCTC | 1 cut(s) | 418 |
| AccI | GTMKAC | 1 cut(s) | 88 |
| AciI | CCGC | 9 cut(s) | 102, 140, 167, 181, 226, 274, 309, 374, 416 |
| AcyI | GRCGYC | 1 cut(s) | 384 |
| AfaI | GTAC | 2 cut(s) | 111, 138 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 98, 190, 273, 274 |
| AgsI | TTSAA | 1 cut(s) | 251 |
| AjnI | CCWGG | 3 cut(s) | 228, 236, 269 |
| AluBI | AGCT | 1 cut(s) | 280 |
| AluI | AGCT | 1 cut(s) | 280 |
| Alw21I | GWGCWC | 1 cut(s) | 77 |
| AoxI | GGCC | 2 cut(s) | 163, 305 |
| ApaI | GGGCCC | 1 cut(s) | 167 |
| ApeKI | GCWGC | 1 cut(s) | 233 |
| Asp718I | GGTACC | 2 cut(s) | 109, 136 |
| AspLEI | GCGC | 1 cut(s) | 31 |
| AspS9I | GGNCC | 3 cut(s) | 163, 164, 306 |
| AsuHPI | GGTGA | 1 cut(s) | 326 |
| BaeGI | GKGCMC | 1 cut(s) | 167 |
| BanI | GGYRCC | 2 cut(s) | 109, 136 |
| BanII | GRGCYC | 1 cut(s) | 167 |
| Bbv12I | GWGCWC | 1 cut(s) | 77 |
| BbvI | GCAGC | 1 cut(s) | 245 |
| BccI | CCATC | 2 cut(s) | 154, 184 |
| BciT130I | CCWGG | 3 cut(s) | 230, 238, 271 |
| BfaI | CTAG | 1 cut(s) | 243 |
| BfoI | RGCGCY | 1 cut(s) | 32 |
| BfuAI | ACCTGC | 2 cut(s) | 124, 271 |
| BisI | GCNGC | 1 cut(s) | 234 |
| BlsI | GCNGC | 1 cut(s) | 235 |
| Bme1390I | CCNGG | 3 cut(s) | 230, 238, 271 |
| BmgT120I | GGNCC | 3 cut(s) | 163, 164, 306 |
| BmiI | GGNNCC | 4 cut(s) | 111, 138, 165, 293 |
| BmrFI | CCNGG | 3 cut(s) | 230, 238, 271 |
| BmsI | GCATC | 3 cut(s) | 71, 120, 333 |
| BpmI | CTGGAG | 1 cut(s) | 281 |
| BsaHI | GRCGYC | 1 cut(s) | 384 |
| BsaJI | CCNNGG | 2 cut(s) | 237, 269 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 53, 83 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 98, 190, 273, 274 |
| Bse1I | ACTGG | 2 cut(s) | 119, 298 |
| BseBI | CCWGG | 3 cut(s) | 230, 238, 271 |
| BseDI | CCNNGG | 2 cut(s) | 237, 269 |
| BseGI | GGATG | 2 cut(s) | 348, 420 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 98, 190, 273, 274 |
| BseNI | ACTGG | 2 cut(s) | 119, 298 |
| BseSI | GKGCMC | 1 cut(s) | 167 |
| BseXI | GCAGC | 1 cut(s) | 245 |
| BshFI | GGCC | 2 cut(s) | 165, 307 |
| BshNI | GGYRCC | 2 cut(s) | 109, 136 |
| BsiHKAI | GWGCWC | 1 cut(s) | 77 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 98, 190, 273, 274 |
| BsnI | GGCC | 2 cut(s) | 165, 307 |
| Bsp120I | GGGCCC | 1 cut(s) | 163 |
| Bsp1286I | GDGCHC | 2 cut(s) | 77, 167 |
| BspACI | CCGC | 9 cut(s) | 102, 140, 167, 181, 226, 274, 309, 374, 416 |
| BspANI | GGCC | 2 cut(s) | 165, 307 |
| BspLI | GGNNCC | 4 cut(s) | 111, 138, 165, 293 |
| BspMI | ACCTGC | 2 cut(s) | 124, 271 |
| BspT107I | GGYRCC | 2 cut(s) | 109, 136 |
| BsrBI | CCGCTC | 1 cut(s) | 418 |
| BsrI | ACTGG | 2 cut(s) | 119, 298 |
| BssECI | CCNNGG | 2 cut(s) | 237, 269 |
| BssNI | GRCGYC | 1 cut(s) | 384 |
| Bst2UI | CCWGG | 3 cut(s) | 230, 238, 271 |
| BstACI | GRCGYC | 1 cut(s) | 384 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 81 |
| BstC8I | GCNNGC | 3 cut(s) | 167, 309, 382 |
| BstDEI | CTNAG | 1 cut(s) | 388 |
| BstF5I | GGATG | 2 cut(s) | 348, 420 |
| BstH2I | RGCGCY | 1 cut(s) | 32 |
| BstHHI | GCGC | 1 cut(s) | 31 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 35, 50, 81, 139, 175, 239 |
| BstNI | CCWGG | 3 cut(s) | 230, 238, 271 |
| BstSCI | CCNGG | 3 cut(s) | 228, 236, 269 |
| BstSLI | GKGCMC | 1 cut(s) | 167 |
| BstV1I | GCAGC | 1 cut(s) | 245 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 229 |
| BsuRI | GGCC | 2 cut(s) | 165, 307 |
| BtsCI | GGATG | 2 cut(s) | 348, 420 |
| BveI | ACCTGC | 2 cut(s) | 124, 271 |
| Cac8I | GCNNGC | 3 cut(s) | 167, 309, 382 |
| CfoI | GCGC | 1 cut(s) | 31 |
| Cfr13I | GGNCC | 3 cut(s) | 163, 164, 306 |
| CseI | GACGC | 1 cut(s) | 373 |
| Csp6I | GTAC | 2 cut(s) | 110, 137 |
| CviJI | RGCY | 8 cut(s) | 165, 203, 214, 236, 242, 280, 307, 364 |
| CviKI_1 | RGCY | 8 cut(s) | 165, 203, 214, 236, 242, 280, 307, 364 |
| CviQI | GTAC | 2 cut(s) | 110, 137 |
| DdeI | CTNAG | 1 cut(s) | 388 |
| EciI | GGCGGA | 2 cut(s) | 117, 389 |
| Eco24I | GRGCYC | 1 cut(s) | 167 |
| EcoRII | CCWGG | 3 cut(s) | 228, 236, 269 |
| EcoT38I | GRGCYC | 1 cut(s) | 167 |
| FaiI | YATR | 3 cut(s) | 116, 125, 427 |
| FauI | CCCGC | 4 cut(s) | 174, 188, 267, 316 |
| FblI | GTMKAC | 1 cut(s) | 88 |
| Fnu4HI | GCNGC | 1 cut(s) | 234 |
| FokI | GGATG | 2 cut(s) | 355, 407 |
| FriOI | GRGCYC | 1 cut(s) | 167 |
| Fsp4HI | GCNGC | 1 cut(s) | 234 |
| FspBI | CTAG | 1 cut(s) | 243 |
| GlaI | GCGC | 1 cut(s) | 30 |
| GluI | GCNGC | 1 cut(s) | 234 |
| GsuI | CTGGAG | 1 cut(s) | 281 |
| HaeII | RGCGCY | 1 cut(s) | 32 |
| HaeIII | GGCC | 2 cut(s) | 165, 307 |
| HgaI | GACGC | 1 cut(s) | 373 |
| HhaI | GCGC | 1 cut(s) | 31 |
| Hin1I | GRCGYC | 1 cut(s) | 384 |
| Hin6I | GCGC | 1 cut(s) | 29 |
| HinP1I | GCGC | 1 cut(s) | 29 |
| HincII | GTYRAC | 1 cut(s) | 89 |
| HindII | GTYRAC | 1 cut(s) | 89 |
| HinfI | GANTC | 1 cut(s) | 206 |
| HphI | GGTGA | 1 cut(s) | 326 |
| Hpy166II | GTNNAC | 1 cut(s) | 89 |
| Hpy188I | TCNGA | 1 cut(s) | 291 |
| Hpy188III | TCNNGA | 1 cut(s) | 334 |
| Hpy8I | GTNNAC | 1 cut(s) | 89 |
| HpyAV | CCTTC | 2 cut(s) | 180, 245 |
| HpyCH4V | TGCA | 3 cut(s) | 84, 133, 346 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 35, 50, 81, 139, 175, 239 |
| HpyF3I | CTNAG | 1 cut(s) | 388 |
| Hsp92I | GRCGYC | 1 cut(s) | 384 |
| HspAI | GCGC | 1 cut(s) | 29 |
| KpnI | GGTACC | 2 cut(s) | 113, 140 |
| LmnI | GCTCC | 4 cut(s) | 61, 72, 193, 277 |
| Lsp1109I | GCAGC | 1 cut(s) | 245 |
| LweI | GCATC | 3 cut(s) | 71, 120, 333 |
| MaeI | CTAG | 1 cut(s) | 243 |
| MaeIII | GTNAC | 1 cut(s) | 314 |
| MbiI | CCGCTC | 1 cut(s) | 418 |
| MhlI | GDGCHC | 2 cut(s) | 77, 167 |
| MluCI | AATT | 3 cut(s) | 17, 246, 350 |
| MnlI | CCTC | 5 cut(s) | 51, 193, 297, 305, 370 |
| MseI | TTAA | 2 cut(s) | 20, 353 |
| MslI | CAYNNNNRTG | 1 cut(s) | 128 |
| MspA1I | CMGCKG | 2 cut(s) | 142, 311 |
| MspR9I | CCNGG | 3 cut(s) | 230, 238, 271 |
| MvaI | CCWGG | 3 cut(s) | 230, 238, 271 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 35, 50, 81, 139, 175, 239 |
| NlaIV | GGNNCC | 4 cut(s) | 111, 138, 165, 293 |
| NmuCI | GTSAC | 1 cut(s) | 314 |
| PaqCI | CACCTGC | 1 cut(s) | 271 |
| PfeI | GAWTC | 1 cut(s) | 206 |
| PkrI | GCNGC | 1 cut(s) | 235 |
| Psp6I | CCWGG | 3 cut(s) | 228, 236, 269 |
| PspGI | CCWGG | 3 cut(s) | 228, 236, 269 |
| PspN4I | GGNNCC | 4 cut(s) | 111, 138, 165, 293 |
| PspOMI | GGGCCC | 1 cut(s) | 163 |
| PspPI | GGNCC | 3 cut(s) | 163, 164, 306 |
| RsaI | GTAC | 2 cut(s) | 111, 138 |
| RsaNI | GTAC | 2 cut(s) | 110, 137 |
| RseI | CAYNNNNRTG | 1 cut(s) | 128 |
| SalI | GTCGAC | 1 cut(s) | 87 |
| SaqAI | TTAA | 2 cut(s) | 20, 353 |
| SatI | GCNGC | 1 cut(s) | 234 |
| Sau96I | GGNCC | 3 cut(s) | 163, 164, 306 |
| ScrFI | CCNGG | 3 cut(s) | 230, 238, 271 |
| SduI | GDGCHC | 2 cut(s) | 77, 167 |
| SetI | ASST | 5 cut(s) | 138, 256, 265, 282, 297 |
| SfaNI | GCATC | 3 cut(s) | 71, 120, 333 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 128 |
| Sse9I | AATT | 3 cut(s) | 17, 246, 350 |
| SsiI | CCGC | 9 cut(s) | 102, 140, 167, 181, 226, 274, 309, 374, 416 |
| SspMI | CTAG | 1 cut(s) | 243 |
| StyD4I | CCNGG | 3 cut(s) | 228, 236, 269 |
| TaqI | TCGA | 3 cut(s) | 12, 88, 302 |
| TasI | AATT | 3 cut(s) | 17, 246, 350 |
| TfiI | GAWTC | 1 cut(s) | 206 |
| Tru1I | TTAA | 2 cut(s) | 20, 353 |
| Tru9I | TTAA | 2 cut(s) | 20, 353 |
| TseFI | GTSAC | 1 cut(s) | 314 |
| TseI | GCWGC | 1 cut(s) | 233 |
| Tsp45I | GTSAC | 1 cut(s) | 314 |
| TspDTI | ATGAA | 2 cut(s) | 30, 275 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 277 |
| XmiI | GTMKAC | 1 cut(s) | 88 |
| XspI | CTAG | 1 cut(s) | 243 |