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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 394, 547 |
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 406 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 1 cut(s) | 277 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcuI | CTGAAG | 3 cut(s) | 48, 311, 546 |
| AfaI | GTAC | 4 cut(s) | 176, 367, 381, 408 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| AjnI | CCWGG | 1 cut(s) | 347 |
| AluBI | AGCT | 1 cut(s) | 181 |
| AluI | AGCT | 1 cut(s) | 181 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 5 cut(s) | 108, 154, 246, 386, 576 |
| ApeKI | GCWGC | 2 cut(s) | 138, 141 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 3 cut(s) | 325, 374, 552 |
| AspS9I | GGNCC | 6 cut(s) | 154, 247, 386, 415, 440, 559 |
| AsuC2I | CCSGG | 5 cut(s) | 107, 117, 153, 363, 384 |
| AvaII | GGWCC | 3 cut(s) | 415, 440, 559 |
| BanI | GGYRCC | 2 cut(s) | 379, 406 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| BbvCI | CCTCAGC | 1 cut(s) | 243 |
| BbvI | GCAGC | 2 cut(s) | 150, 153 |
| BccI | CCATC | 3 cut(s) | 59, 430, 458 |
| BciT130I | CCWGG | 1 cut(s) | 349 |
| BcnI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 384 |
| BfuAI | ACCTGC | 2 cut(s) | 394, 547 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 3 cut(s) | 139, 142, 491 |
| BlsI | GCNGC | 3 cut(s) | 140, 143, 492 |
| Bme1390I | CCNGG | 6 cut(s) | 107, 117, 153, 349, 363, 384 |
| Bme18I | GGWCC | 3 cut(s) | 415, 440, 559 |
| BmgT120I | GGNCC | 6 cut(s) | 154, 247, 386, 415, 440, 559 |
| BmiI | GGNNCC | 6 cut(s) | 381, 387, 408, 417, 441, 442 |
| BmrFI | CCNGG | 6 cut(s) | 107, 117, 153, 349, 363, 384 |
| BmsI | GCATC | 2 cut(s) | 390, 583 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| Bpu10I | CCTNAGC | 1 cut(s) | 243 |
| BpuMI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 384 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaJI | CCNNGG | 4 cut(s) | 105, 115, 250, 383 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| Bse1I | ACTGG | 3 cut(s) | 192, 373, 389 |
| Bse3DI | GCAATG | 1 cut(s) | 485 |
| BseBI | CCWGG | 1 cut(s) | 349 |
| BseDI | CCNNGG | 4 cut(s) | 105, 115, 250, 383 |
| BseGI | GGATG | 4 cut(s) | 51, 441, 450, 624 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| BseMI | GCAATG | 1 cut(s) | 485 |
| BseMII | CTCAG | 3 cut(s) | 234, 414, 536 |
| BseNI | ACTGG | 3 cut(s) | 192, 373, 389 |
| BseRI | GAGGAG | 1 cut(s) | 236 |
| BseXI | GCAGC | 2 cut(s) | 150, 153 |
| BseYI | CCCAGC | 2 cut(s) | 57, 70 |
| BsgI | GTGCAG | 1 cut(s) | 525 |
| Bsh1236I | CGCG | 1 cut(s) | 277 |
| Bsh1285I | CGRYCG | 1 cut(s) | 312 |
| BshFI | GGCC | 5 cut(s) | 110, 156, 248, 388, 578 |
| BshNI | GGYRCC | 2 cut(s) | 379, 406 |
| BsiEI | CGRYCG | 1 cut(s) | 312 |
| BsiSI | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsnI | GGCC | 5 cut(s) | 110, 156, 248, 388, 578 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspANI | GGCC | 5 cut(s) | 110, 156, 248, 388, 578 |
| BspCNI | CTCAG | 3 cut(s) | 235, 415, 537 |
| BspFNI | CGCG | 1 cut(s) | 277 |
| BspLI | GGNNCC | 6 cut(s) | 381, 387, 408, 417, 441, 442 |
| BspMI | ACCTGC | 2 cut(s) | 394, 547 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 406 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrDI | GCAATG | 1 cut(s) | 485 |
| BsrI | ACTGG | 3 cut(s) | 192, 373, 389 |
| BssECI | CCNNGG | 4 cut(s) | 105, 115, 250, 383 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| BssT1I | CCWWGG | 1 cut(s) | 250 |
| Bst2UI | CCWGG | 1 cut(s) | 349 |
| Bst4CI | ACNGT | 4 cut(s) | 11, 44, 258, 336 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 540, 554, 589 |
| BstDEI | CTNAG | 3 cut(s) | 243, 423, 545 |
| BstF5I | GGATG | 4 cut(s) | 51, 441, 450, 624 |
| BstFNI | CGCG | 1 cut(s) | 277 |
| BstHHI | GCGC | 3 cut(s) | 325, 374, 552 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMCI | CGRYCG | 1 cut(s) | 312 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 274, 320, 409, 562, 584, 593 |
| BstNI | CCWGG | 1 cut(s) | 349 |
| BstSCI | CCNGG | 6 cut(s) | 105, 115, 151, 347, 361, 382 |
| BstUI | CGCG | 1 cut(s) | 277 |
| BstV1I | GCAGC | 2 cut(s) | 150, 153 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 5 cut(s) | 110, 156, 248, 388, 578 |
| BtgZI | GCGATG | 1 cut(s) | 606 |
| BtsCI | GGATG | 4 cut(s) | 51, 441, 450, 624 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 542 |
| BveI | ACCTGC | 2 cut(s) | 394, 547 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 540, 554, 589 |
| CfoI | GCGC | 3 cut(s) | 325, 374, 552 |
| Cfr13I | GGNCC | 6 cut(s) | 154, 247, 386, 415, 440, 559 |
| Csp6I | GTAC | 4 cut(s) | 175, 366, 380, 407 |
| CviQI | GTAC | 4 cut(s) | 175, 366, 380, 407 |
| DdeI | CTNAG | 3 cut(s) | 243, 423, 545 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 3 cut(s) | 37, 206, 525 |
| Eco130I | CCWWGG | 1 cut(s) | 250 |
| Eco47I | GGWCC | 3 cut(s) | 415, 440, 559 |
| Eco57I | CTGAAG | 3 cut(s) | 48, 311, 546 |
| EcoO109I | RGGNCCY | 1 cut(s) | 440 |
| EcoRII | CCWGG | 1 cut(s) | 347 |
| EcoT14I | CCWWGG | 1 cut(s) | 250 |
| ErhI | CCWWGG | 1 cut(s) | 250 |
| FaiI | YATR | 3 cut(s) | 173, 201, 395 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FauI | CCCGC | 2 cut(s) | 8, 156 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 3 cut(s) | 139, 142, 491 |
| FokI | GGATG | 3 cut(s) | 38, 437, 448 |
| Fsp4HI | GCNGC | 3 cut(s) | 139, 142, 491 |
| GlaI | GCGC | 3 cut(s) | 324, 373, 551 |
| GluI | GCNGC | 3 cut(s) | 139, 142, 491 |
| GsaI | CCCAGC | 2 cut(s) | 61, 74 |
| HaeIII | GGCC | 5 cut(s) | 110, 156, 248, 388, 578 |
| HapII | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| HhaI | GCGC | 3 cut(s) | 325, 374, 552 |
| Hin6I | GCGC | 3 cut(s) | 323, 372, 550 |
| HinP1I | GCGC | 3 cut(s) | 323, 372, 550 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 508 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| HpyAV | CCTTC | 3 cut(s) | 232, 503, 521 |
| HpyCH4III | ACNGT | 4 cut(s) | 11, 44, 258, 336 |
| HpyCH4IV | ACGT | 2 cut(s) | 217, 340 |
| HpyCH4V | TGCA | 3 cut(s) | 403, 542, 596 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 274, 320, 409, 562, 584, 593 |
| HpyF3I | CTNAG | 3 cut(s) | 243, 423, 545 |
| HpySE526I | ACGT | 2 cut(s) | 217, 340 |
| HspAI | GCGC | 3 cut(s) | 323, 372, 550 |
| KflI | GGGWCCC | 1 cut(s) | 440 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| Lsp1109I | GCAGC | 2 cut(s) | 150, 153 |
| LweI | GCATC | 2 cut(s) | 390, 583 |
| MaeII | ACGT | 2 cut(s) | 217, 340 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 4 cut(s) | 41, 188, 304, 307 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 2 cut(s) | 90, 591 |
| MnlI | CCTC | 7 cut(s) | 73, 118, 214, 217, 238, 513, 540 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 2 cut(s) | 170, 398 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 412, 565 |
| MspI | CCGG | 5 cut(s) | 107, 117, 152, 363, 383 |
| MspR9I | CCNGG | 6 cut(s) | 107, 117, 153, 349, 363, 384 |
| MvaI | CCWGG | 1 cut(s) | 349 |
| MvnI | CGCG | 1 cut(s) | 277 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 274, 320, 409, 562, 584, 593 |
| NciI | CCSGG | 5 cut(s) | 107, 117, 153, 363, 384 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| NlaIV | GGNNCC | 6 cut(s) | 381, 387, 408, 417, 441, 442 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 482 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 3 cut(s) | 140, 143, 492 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| PpuMI | RGGWCCY | 1 cut(s) | 440 |
| Psp5II | RGGWCCY | 1 cut(s) | 440 |
| Psp6I | CCWGG | 1 cut(s) | 347 |
| PspFI | CCCAGC | 2 cut(s) | 57, 70 |
| PspGI | CCWGG | 1 cut(s) | 347 |
| PspN4I | GGNNCC | 6 cut(s) | 381, 387, 408, 417, 441, 442 |
| PspPI | GGNCC | 6 cut(s) | 154, 247, 386, 415, 440, 559 |
| PspPPI | RGGWCCY | 1 cut(s) | 440 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 4 cut(s) | 176, 367, 381, 408 |
| RsaNI | GTAC | 4 cut(s) | 175, 366, 380, 407 |
| RseI | CAYNNNNRTG | 2 cut(s) | 170, 398 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 3 cut(s) | 139, 142, 491 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 6 cut(s) | 154, 247, 386, 415, 440, 559 |
| ScrFI | CCNGG | 6 cut(s) | 107, 117, 153, 349, 363, 384 |
| SetI | ASST | 7 cut(s) | 183, 220, 343, 408, 424, 514, 561 |
| SfaNI | GCATC | 2 cut(s) | 390, 583 |
| SinI | GGWCC | 3 cut(s) | 415, 440, 559 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 170, 398 |
| SspI | AATATT | 1 cut(s) | 571 |
| StyD4I | CCNGG | 6 cut(s) | 105, 115, 151, 347, 361, 382 |
| StyI | CCWWGG | 1 cut(s) | 250 |
| TaaI | ACNGT | 4 cut(s) | 11, 44, 258, 336 |
| TaiI | ACGT | 2 cut(s) | 220, 343 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TauI | GCSGC | 1 cut(s) | 493 |
| TfiI | GAWTC | 1 cut(s) | 482 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 192, 549 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 2 cut(s) | 138, 141 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspDTI | ATGAA | 2 cut(s) | 188, 300 |
| TspRI | CASTG | 2 cut(s) | 192, 549 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 3 cut(s) | 415, 440, 559 |
| XmiI | GTMKAC | 1 cut(s) | 358 |