No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 313 |
| Acc36I | ACCTGC | 2 cut(s) | 161, 313 |
| Acc65I | GGTACC | 1 cut(s) | 22 |
| AccB1I | GGYRCC | 1 cut(s) | 22 |
| AccBSI | CCGCTC | 3 cut(s) | 570, 608, 668 |
| AccII | CGCG | 4 cut(s) | 113, 123, 285, 343 |
| AclWI | GGATC | 2 cut(s) | 267, 628 |
| AcoI | YGGCCR | 1 cut(s) | 327 |
| AcuI | CTGAAG | 1 cut(s) | 186 |
| AfaI | GTAC | 4 cut(s) | 12, 24, 519, 637 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 38, 66, 322, 434, 612, 617 |
| AjnI | CCWGG | 5 cut(s) | 62, 217, 413, 427, 611 |
| AleI | CACNNNNGTG | 1 cut(s) | 335 |
| AluBI | AGCT | 4 cut(s) | 179, 355, 493, 526 |
| AluI | AGCT | 4 cut(s) | 179, 355, 493, 526 |
| Alw21I | GWGCWC | 1 cut(s) | 413 |
| Alw26I | GTCTC | 1 cut(s) | 568 |
| AlwI | GGATC | 2 cut(s) | 267, 628 |
| Ama87I | CYCGRG | 1 cut(s) | 571 |
| AoxI | GGCC | 5 cut(s) | 220, 312, 327, 506, 646 |
| ApeKI | GCWGC | 2 cut(s) | 207, 478 |
| Asp718I | GGTACC | 1 cut(s) | 22 |
| AspA2I | CCTAGG | 1 cut(s) | 578 |
| AspLEI | GCGC | 4 cut(s) | 115, 125, 285, 345 |
| AspS9I | GGNCC | 4 cut(s) | 173, 313, 506, 647 |
| AsuC2I | CCSGG | 2 cut(s) | 386, 640 |
| AsuHPI | GGTGA | 1 cut(s) | 557 |
| AsuNHI | GCTAGC | 1 cut(s) | 493 |
| AvaI | CYCGRG | 1 cut(s) | 571 |
| AvaII | GGWCC | 1 cut(s) | 173 |
| AvrII | CCTAGG | 1 cut(s) | 578 |
| BalI | TGGCCA | 1 cut(s) | 329 |
| BanI | GGYRCC | 1 cut(s) | 22 |
| Bbv12I | GWGCWC | 1 cut(s) | 413 |
| BbvI | GCAGC | 2 cut(s) | 219, 465 |
| BccI | CCATC | 1 cut(s) | 129 |
| BciT130I | CCWGG | 5 cut(s) | 64, 219, 415, 429, 613 |
| BcnI | CCSGG | 2 cut(s) | 386, 640 |
| BcoDI | GTCTC | 1 cut(s) | 568 |
| BfaI | CTAG | 3 cut(s) | 494, 579, 644 |
| BfuAI | ACCTGC | 2 cut(s) | 161, 313 |
| BglI | GCCNNNNNGGC | 4 cut(s) | 68, 136, 386, 654 |
| BisI | GCNGC | 5 cut(s) | 105, 116, 208, 479, 509 |
| BlnI | CCTAGG | 1 cut(s) | 578 |
| BlsI | GCNGC | 5 cut(s) | 106, 117, 209, 480, 510 |
| Bme1390I | CCNGG | 7 cut(s) | 64, 219, 386, 415, 429, 613, 640 |
| Bme18I | GGWCC | 1 cut(s) | 173 |
| BmeT110I | CYCGRG | 1 cut(s) | 571 |
| BmgT120I | GGNCC | 4 cut(s) | 173, 313, 506, 647 |
| BmiI | GGNNCC | 3 cut(s) | 24, 507, 649 |
| BmrFI | CCNGG | 7 cut(s) | 64, 219, 386, 415, 429, 613, 640 |
| BmrI | ACTGGG | 1 cut(s) | 167 |
| BmsI | GCATC | 3 cut(s) | 135, 148, 649 |
| BmtI | GCTAGC | 1 cut(s) | 497 |
| BmuI | ACTGGG | 1 cut(s) | 167 |
| BpmI | CTGGAG | 1 cut(s) | 80 |
| BpuMI | CCSGG | 2 cut(s) | 386, 640 |
| BsaI | GGTCTC | 1 cut(s) | 568 |
| BsaJI | CCNNGG | 5 cut(s) | 78, 309, 578, 591, 611 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 533, 563 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 38, 66, 322, 434, 612, 617 |
| Bse118I | RCCGGY | 1 cut(s) | 653 |
| Bse1I | ACTGG | 4 cut(s) | 162, 258, 455, 681 |
| BseBI | CCWGG | 5 cut(s) | 64, 219, 415, 429, 613 |
| BseDI | CCNNGG | 5 cut(s) | 78, 309, 578, 591, 611 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 38, 66, 322, 434, 612, 617 |
| BseNI | ACTGG | 4 cut(s) | 162, 258, 455, 681 |
| BseXI | GCAGC | 2 cut(s) | 219, 465 |
| BseYI | CCCAGC | 1 cut(s) | 522 |
| BsgI | GTGCAG | 2 cut(s) | 464, 542 |
| Bsh1236I | CGCG | 4 cut(s) | 113, 123, 285, 343 |
| Bsh1285I | CGRYCG | 1 cut(s) | 215 |
| BshFI | GGCC | 5 cut(s) | 222, 314, 329, 508, 648 |
| BshNI | GGYRCC | 1 cut(s) | 22 |
| BsiEI | CGRYCG | 1 cut(s) | 215 |
| BsiHKAI | GWGCWC | 1 cut(s) | 413 |
| BsiHKCI | CYCGRG | 1 cut(s) | 571 |
| BsiSI | CCGG | 4 cut(s) | 186, 386, 640, 654 |
| BslFI | GGGAC | 1 cut(s) | 458 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 38, 66, 322, 434, 612, 617 |
| BsmAI | GTCTC | 1 cut(s) | 568 |
| BsmFI | GGGAC | 1 cut(s) | 458 |
| BsnI | GGCC | 5 cut(s) | 222, 314, 329, 508, 648 |
| Bso31I | GGTCTC | 1 cut(s) | 568 |
| BsoBI | CYCGRG | 1 cut(s) | 571 |
| Bsp1286I | GDGCHC | 1 cut(s) | 413 |
| Bsp143I | GATC | 2 cut(s) | 259, 620 |
| BspANI | GGCC | 5 cut(s) | 222, 314, 329, 508, 648 |
| BspFNI | CGCG | 4 cut(s) | 113, 123, 285, 343 |
| BspLI | GGNNCC | 3 cut(s) | 24, 507, 649 |
| BspMI | ACCTGC | 2 cut(s) | 161, 313 |
| BspOI | GCTAGC | 1 cut(s) | 497 |
| BspPI | GGATC | 2 cut(s) | 267, 628 |
| BspT107I | GGYRCC | 1 cut(s) | 22 |
| BspTNI | GGTCTC | 1 cut(s) | 568 |
| BsrBI | CCGCTC | 3 cut(s) | 570, 608, 668 |
| BsrFI | RCCGGY | 1 cut(s) | 653 |
| BsrI | ACTGG | 4 cut(s) | 162, 258, 455, 681 |
| BssAI | RCCGGY | 1 cut(s) | 653 |
| BssECI | CCNNGG | 5 cut(s) | 78, 309, 578, 591, 611 |
| BssMI | GATC | 2 cut(s) | 259, 620 |
| BssT1I | CCWWGG | 1 cut(s) | 578 |
| Bst2UI | CCWGG | 5 cut(s) | 64, 219, 415, 429, 613 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 145 |
| BstC8I | GCNNGC | 4 cut(s) | 69, 398, 495, 655 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 432 |
| BstFNI | CGCG | 4 cut(s) | 113, 123, 285, 343 |
| BstHHI | GCGC | 4 cut(s) | 115, 125, 285, 345 |
| BstKTI | GATC | 2 cut(s) | 262, 623 |
| BstMAI | GTCTC | 1 cut(s) | 568 |
| BstMBI | GATC | 2 cut(s) | 259, 620 |
| BstMCI | CGRYCG | 1 cut(s) | 215 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 68, 110, 136, 145, 176, 386, 475, 654, 659 |
| BstNI | CCWGG | 5 cut(s) | 64, 219, 415, 429, 613 |
| BstNSI | RCATGY | 1 cut(s) | 51 |
| BstSCI | CCNGG | 7 cut(s) | 62, 217, 384, 413, 427, 611, 638 |
| BstUI | CGCG | 4 cut(s) | 113, 123, 285, 343 |
| BstV1I | GCAGC | 2 cut(s) | 219, 465 |
| BstX2I | RGATCY | 1 cut(s) | 259 |
| BstYI | RGATCY | 1 cut(s) | 259 |
| BsuRI | GGCC | 5 cut(s) | 222, 314, 329, 508, 648 |
| BtgZI | GCGATG | 1 cut(s) | 672 |
| BtsI | GCAGTG | 1 cut(s) | 554 |
| BtsIMutI | CAGTG | 2 cut(s) | 251, 554 |
| BveI | ACCTGC | 2 cut(s) | 161, 313 |
| Cac8I | GCNNGC | 4 cut(s) | 69, 398, 495, 655 |
| CfoI | GCGC | 4 cut(s) | 115, 125, 285, 345 |
| Cfr10I | RCCGGY | 1 cut(s) | 653 |
| Cfr13I | GGNCC | 4 cut(s) | 173, 313, 506, 647 |
| CseI | GACGC | 1 cut(s) | 119 |
| CsiI | ACCWGGT | 1 cut(s) | 427 |
| Csp6I | GTAC | 4 cut(s) | 11, 23, 518, 636 |
| CviAII | CATG | 2 cut(s) | 48, 515 |
| CviQI | GTAC | 4 cut(s) | 11, 23, 518, 636 |
| DpnI | GATC | 2 cut(s) | 261, 622 |
| DpnII | GATC | 2 cut(s) | 259, 620 |
| EaeI | YGGCCR | 1 cut(s) | 327 |
| EciI | GGCGGA | 1 cut(s) | 272 |
| Eco130I | CCWWGG | 1 cut(s) | 578 |
| Eco31I | GGTCTC | 1 cut(s) | 568 |
| Eco47I | GGWCC | 1 cut(s) | 173 |
| Eco57I | CTGAAG | 1 cut(s) | 186 |
| Eco88I | CYCGRG | 1 cut(s) | 571 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 432 |
| EcoO109I | RGGNCCY | 1 cut(s) | 647 |
| EcoRII | CCWGG | 5 cut(s) | 62, 217, 413, 427, 611 |
| EcoT14I | CCWWGG | 1 cut(s) | 578 |
| ErhI | CCWWGG | 1 cut(s) | 578 |
| FaeI | CATG | 2 cut(s) | 51, 518 |
| FaiI | YATR | 7 cut(s) | 49, 241, 243, 434, 465, 467, 516 |
| FaqI | GGGAC | 1 cut(s) | 458 |
| FatI | CATG | 2 cut(s) | 47, 514 |
| FauI | CCCGC | 5 cut(s) | 60, 483, 490, 613, 658 |
| FauNDI | CATATG | 1 cut(s) | 465 |
| Fnu4HI | GCNGC | 5 cut(s) | 105, 116, 208, 479, 509 |
| Fsp4HI | GCNGC | 5 cut(s) | 105, 116, 208, 479, 509 |
| FspBI | CTAG | 3 cut(s) | 494, 579, 644 |
| GlaI | GCGC | 4 cut(s) | 114, 124, 284, 344 |
| GluI | GCNGC | 5 cut(s) | 105, 116, 208, 479, 509 |
| GsaI | CCCAGC | 1 cut(s) | 526 |
| GsuI | CTGGAG | 1 cut(s) | 80 |
| HaeIII | GGCC | 5 cut(s) | 222, 314, 329, 508, 648 |
| HapII | CCGG | 4 cut(s) | 186, 386, 640, 654 |
| HgaI | GACGC | 1 cut(s) | 119 |
| HhaI | GCGC | 4 cut(s) | 115, 125, 285, 345 |
| Hin1II | CATG | 2 cut(s) | 51, 518 |
| Hin6I | GCGC | 4 cut(s) | 113, 123, 283, 343 |
| HinP1I | GCGC | 4 cut(s) | 113, 123, 283, 343 |
| HinfI | GANTC | 2 cut(s) | 83, 548 |
| HpaII | CCGG | 4 cut(s) | 186, 386, 640, 654 |
| HphI | GGTGA | 1 cut(s) | 557 |
| Hpy188I | TCNGA | 4 cut(s) | 82, 88, 144, 153 |
| Hpy188III | TCNNGA | 3 cut(s) | 97, 263, 573 |
| Hpy99I | CGWCG | 1 cut(s) | 113 |
| HpyCH4V | TGCA | 6 cut(s) | 51, 463, 469, 481, 559, 662 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 68, 110, 136, 145, 176, 386, 475, 654, 659 |
| Hsp92II | CATG | 2 cut(s) | 51, 518 |
| HspAI | GCGC | 4 cut(s) | 113, 123, 283, 343 |
| KpnI | GGTACC | 1 cut(s) | 26 |
| KroI | GCCGGC | 1 cut(s) | 653 |
| KroNI | GCCGGC | 1 cut(s) | 655 |
| Kzo9I | GATC | 2 cut(s) | 259, 620 |
| LmnI | GCTCC | 3 cut(s) | 408, 437, 613 |
| Lsp1109I | GCAGC | 2 cut(s) | 219, 465 |
| LweI | GCATC | 3 cut(s) | 135, 148, 649 |
| MabI | ACCWGGT | 1 cut(s) | 427 |
| MaeI | CTAG | 3 cut(s) | 494, 579, 644 |
| MaeIII | GTNAC | 1 cut(s) | 402 |
| MalI | GATC | 2 cut(s) | 261, 622 |
| MbiI | CCGCTC | 3 cut(s) | 570, 608, 668 |
| MboI | GATC | 2 cut(s) | 259, 620 |
| MflI | RGATCY | 1 cut(s) | 259 |
| MhlI | GDGCHC | 1 cut(s) | 413 |
| MlsI | TGGCCA | 1 cut(s) | 329 |
| MluCI | AATT | 1 cut(s) | 27 |
| MluNI | TGGCCA | 1 cut(s) | 329 |
| MlyI | GAGTC | 2 cut(s) | 92, 557 |
| MmeI | TCCRAC | 1 cut(s) | 513 |
| MnlI | CCTC | 7 cut(s) | 88, 104, 239, 244, 304, 535, 586 |
| Mox20I | TGGCCA | 1 cut(s) | 329 |
| MreI | CGCCGGCG | 1 cut(s) | 653 |
| MroNI | GCCGGC | 1 cut(s) | 653 |
| MscI | TGGCCA | 1 cut(s) | 329 |
| MseI | TTAA | 1 cut(s) | 201 |
| MslI | CAYNNNNRTG | 1 cut(s) | 335 |
| Msp20I | TGGCCA | 1 cut(s) | 329 |
| MspA1I | CMGCKG | 2 cut(s) | 478, 631 |
| MspI | CCGG | 4 cut(s) | 186, 386, 640, 654 |
| MspR9I | CCNGG | 7 cut(s) | 64, 219, 386, 415, 429, 613, 640 |
| MvaI | CCWGG | 5 cut(s) | 64, 219, 415, 429, 613 |
| MvnI | CGCG | 4 cut(s) | 113, 123, 285, 343 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 68, 110, 136, 145, 176, 386, 475, 654, 659 |
| NaeI | GCCGGC | 1 cut(s) | 655 |
| NciI | CCSGG | 2 cut(s) | 386, 640 |
| NdeI | CATATG | 1 cut(s) | 465 |
| NdeII | GATC | 2 cut(s) | 259, 620 |
| NgoMIV | GCCGGC | 1 cut(s) | 653 |
| NheI | GCTAGC | 1 cut(s) | 493 |
| NlaIII | CATG | 2 cut(s) | 51, 518 |
| NlaIV | GGNNCC | 3 cut(s) | 24, 507, 649 |
| NmeAIII | GCCGAG | 1 cut(s) | 334 |
| NspI | RCATGY | 1 cut(s) | 51 |
| OliI | CACNNNNGTG | 1 cut(s) | 335 |
| PaeR7I | CTCGAG | 1 cut(s) | 571 |
| PaqCI | CACCTGC | 1 cut(s) | 313 |
| PdiI | GCCGGC | 1 cut(s) | 655 |
| PkrI | GCNGC | 5 cut(s) | 106, 117, 209, 480, 510 |
| PleI | GAGTC | 2 cut(s) | 91, 556 |
| PpsI | GAGTC | 2 cut(s) | 91, 556 |
| Psp6I | CCWGG | 5 cut(s) | 62, 217, 413, 427, 611 |
| PspFI | CCCAGC | 1 cut(s) | 522 |
| PspGI | CCWGG | 5 cut(s) | 62, 217, 413, 427, 611 |
| PspN4I | GGNNCC | 3 cut(s) | 24, 507, 649 |
| PspPI | GGNCC | 4 cut(s) | 173, 313, 506, 647 |
| PsuI | RGATCY | 1 cut(s) | 259 |
| RsaI | GTAC | 4 cut(s) | 12, 24, 519, 637 |
| RsaNI | GTAC | 4 cut(s) | 11, 23, 518, 636 |
| RseI | CAYNNNNRTG | 1 cut(s) | 335 |
| SaqAI | TTAA | 1 cut(s) | 201 |
| SatI | GCNGC | 5 cut(s) | 105, 116, 208, 479, 509 |
| Sau3AI | GATC | 2 cut(s) | 259, 620 |
| Sau96I | GGNCC | 4 cut(s) | 173, 313, 506, 647 |
| SchI | GAGTC | 2 cut(s) | 92, 557 |
| ScrFI | CCNGG | 7 cut(s) | 64, 219, 386, 415, 429, 613, 640 |
| SduI | GDGCHC | 1 cut(s) | 413 |
| SexAI | ACCWGGT | 1 cut(s) | 427 |
| SfaNI | GCATC | 3 cut(s) | 135, 148, 649 |
| Sfr274I | CTCGAG | 1 cut(s) | 571 |
| SgrAI | CRCCGGYG | 1 cut(s) | 653 |
| SinI | GGWCC | 1 cut(s) | 173 |
| SlaI | CTCGAG | 1 cut(s) | 571 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 335 |
| SmlI | CTYRAG | 1 cut(s) | 571 |
| SmoI | CTYRAG | 1 cut(s) | 571 |
| Sse9I | AATT | 1 cut(s) | 27 |
| SspMI | CTAG | 3 cut(s) | 494, 579, 644 |
| StyD4I | CCNGG | 7 cut(s) | 62, 217, 384, 413, 427, 611, 638 |
| StyI | CCWWGG | 1 cut(s) | 578 |
| TaqI | TCGA | 1 cut(s) | 572 |
| TasI | AATT | 1 cut(s) | 27 |
| TauI | GCSGC | 3 cut(s) | 107, 118, 511 |
| Tru1I | TTAA | 1 cut(s) | 201 |
| Tru9I | TTAA | 1 cut(s) | 201 |
| TscAI | CASTG | 2 cut(s) | 258, 561 |
| TseI | GCWGC | 2 cut(s) | 207, 478 |
| TspGWI | ACGGA | 1 cut(s) | 421 |
| TspRI | CASTG | 2 cut(s) | 258, 561 |
| VpaK11BI | GGWCC | 1 cut(s) | 173 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 432 |
| XceI | RCATGY | 1 cut(s) | 51 |
| XhoI | CTCGAG | 1 cut(s) | 571 |
| XmaJI | CCTAGG | 1 cut(s) | 578 |
| XspI | CTAG | 3 cut(s) | 494, 579, 644 |