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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 318 |
| AccB1I | GGYRCC | 1 cut(s) | 39 |
| AccBSI | CCGCTC | 1 cut(s) | 258 |
| AccII | CGCG | 1 cut(s) | 178 |
| AciI | CCGC | 9 cut(s) | 14, 103, 126, 136, 162, 176, 208, 256, 279 |
| AcuI | CTGAAG | 1 cut(s) | 136 |
| AcyI | GRCGYC | 1 cut(s) | 40 |
| AfaI | GTAC | 1 cut(s) | 264 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 165, 239, 290 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 67 |
| AluBI | AGCT | 1 cut(s) | 269 |
| AluI | AGCT | 1 cut(s) | 269 |
| AoxI | GGCC | 2 cut(s) | 242, 328 |
| ApeKI | GCWGC | 1 cut(s) | 229 |
| AspLEI | GCGC | 3 cut(s) | 42, 180, 319 |
| AspS9I | GGNCC | 3 cut(s) | 128, 242, 281 |
| AsuC2I | CCSGG | 2 cut(s) | 205, 241 |
| AsuHPI | GGTGA | 2 cut(s) | 222, 245 |
| AvaII | GGWCC | 2 cut(s) | 128, 281 |
| BanI | GGYRCC | 1 cut(s) | 39 |
| BbvI | GCAGC | 1 cut(s) | 241 |
| BceAI | ACGGC | 2 cut(s) | 27, 123 |
| BcnI | CCSGG | 2 cut(s) | 205, 241 |
| BfaI | CTAG | 1 cut(s) | 332 |
| BfoI | RGCGCY | 1 cut(s) | 43 |
| BglII | AGATCT | 1 cut(s) | 248 |
| BisI | GCNGC | 1 cut(s) | 230 |
| BlsI | GCNGC | 1 cut(s) | 231 |
| Bme1390I | CCNGG | 2 cut(s) | 205, 241 |
| Bme18I | GGWCC | 2 cut(s) | 128, 281 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 67 |
| BmgT120I | GGNCC | 3 cut(s) | 128, 242, 281 |
| BmiI | GGNNCC | 1 cut(s) | 41 |
| BmrFI | CCNGG | 2 cut(s) | 205, 241 |
| BpuMI | CCSGG | 2 cut(s) | 205, 241 |
| BsaHI | GRCGYC | 1 cut(s) | 40 |
| BsaJI | CCNNGG | 2 cut(s) | 57, 203 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 165, 239, 290 |
| Bse1I | ACTGG | 2 cut(s) | 131, 280 |
| BseDI | CCNNGG | 2 cut(s) | 57, 203 |
| BseGI | GGATG | 1 cut(s) | 71 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 165, 239, 290 |
| BseNI | ACTGG | 2 cut(s) | 131, 280 |
| BseXI | GCAGC | 1 cut(s) | 241 |
| BseYI | CCCAGC | 2 cut(s) | 158, 194 |
| Bsh1236I | CGCG | 1 cut(s) | 178 |
| BshFI | GGCC | 2 cut(s) | 244, 330 |
| BshNI | GGYRCC | 1 cut(s) | 39 |
| BsiSI | CCGG | 2 cut(s) | 205, 240 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 165, 239, 290 |
| BsnI | GGCC | 2 cut(s) | 244, 330 |
| Bsp143I | GATC | 1 cut(s) | 248 |
| BspACI | CCGC | 9 cut(s) | 14, 103, 126, 136, 162, 176, 208, 256, 279 |
| BspANI | GGCC | 2 cut(s) | 244, 330 |
| BspFNI | CGCG | 1 cut(s) | 178 |
| BspHI | TCATGA | 1 cut(s) | 88 |
| BspLI | GGNNCC | 1 cut(s) | 41 |
| BspQI | GCTCTTC | 1 cut(s) | 112 |
| BspT107I | GGYRCC | 1 cut(s) | 39 |
| BsrBI | CCGCTC | 1 cut(s) | 258 |
| BsrI | ACTGG | 2 cut(s) | 131, 280 |
| BssECI | CCNNGG | 2 cut(s) | 57, 203 |
| BssMI | GATC | 1 cut(s) | 248 |
| BssNI | GRCGYC | 1 cut(s) | 40 |
| BssT1I | CCWWGG | 1 cut(s) | 57 |
| Bst4CI | ACNGT | 1 cut(s) | 99 |
| Bst6I | CTCTTC | 1 cut(s) | 112 |
| BstACI | GRCGYC | 1 cut(s) | 40 |
| BstC8I | GCNNGC | 2 cut(s) | 124, 271 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 288 |
| BstF5I | GGATG | 1 cut(s) | 71 |
| BstFNI | CGCG | 1 cut(s) | 178 |
| BstH2I | RGCGCY | 1 cut(s) | 43 |
| BstHHI | GCGC | 3 cut(s) | 42, 180, 319 |
| BstKTI | GATC | 1 cut(s) | 251 |
| BstMBI | GATC | 1 cut(s) | 248 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 175 |
| BstSCI | CCNGG | 2 cut(s) | 203, 239 |
| BstUI | CGCG | 1 cut(s) | 178 |
| BstV1I | GCAGC | 1 cut(s) | 241 |
| BstX2I | RGATCY | 1 cut(s) | 248 |
| BstYI | RGATCY | 1 cut(s) | 248 |
| BsuRI | GGCC | 2 cut(s) | 244, 330 |
| BtsCI | GGATG | 1 cut(s) | 71 |
| BtsIMutI | CAGTG | 1 cut(s) | 273 |
| Cac8I | GCNNGC | 2 cut(s) | 124, 271 |
| CciI | TCATGA | 1 cut(s) | 88 |
| CfoI | GCGC | 3 cut(s) | 42, 180, 319 |
| Cfr13I | GGNCC | 3 cut(s) | 128, 242, 281 |
| Csp6I | GTAC | 1 cut(s) | 263 |
| CviAII | CATG | 1 cut(s) | 89 |
| CviJI | RGCY | 6 cut(s) | 10, 198, 232, 244, 269, 330 |
| CviKI_1 | RGCY | 6 cut(s) | 10, 198, 232, 244, 269, 330 |
| CviQI | GTAC | 1 cut(s) | 263 |
| DinI | GGCGCC | 1 cut(s) | 41 |
| DpnI | GATC | 1 cut(s) | 250 |
| DpnII | GATC | 1 cut(s) | 248 |
| DriI | GACNNNNNGTC | 1 cut(s) | 67 |
| Eam1104I | CTCTTC | 1 cut(s) | 112 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 67 |
| EarI | CTCTTC | 1 cut(s) | 112 |
| EciI | GGCGGA | 3 cut(s) | 125, 141, 294 |
| Eco130I | CCWWGG | 1 cut(s) | 57 |
| Eco147I | AGGCCT | 1 cut(s) | 330 |
| Eco47I | GGWCC | 2 cut(s) | 128, 281 |
| Eco57I | CTGAAG | 1 cut(s) | 136 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 288 |
| EcoT14I | CCWWGG | 1 cut(s) | 57 |
| EgeI | GGCGCC | 1 cut(s) | 41 |
| EheI | GGCGCC | 1 cut(s) | 41 |
| ErhI | CCWWGG | 1 cut(s) | 57 |
| FaeI | CATG | 1 cut(s) | 92 |
| FaiI | YATR | 1 cut(s) | 90 |
| FatI | CATG | 1 cut(s) | 88 |
| FauI | CCCGC | 1 cut(s) | 96 |
| Fnu4HI | GCNGC | 1 cut(s) | 230 |
| FokI | GGATG | 1 cut(s) | 58 |
| Fsp4HI | GCNGC | 1 cut(s) | 230 |
| FspBI | CTAG | 1 cut(s) | 332 |
| FspI | TGCGCA | 1 cut(s) | 318 |
| GlaI | GCGC | 3 cut(s) | 41, 179, 318 |
| GluI | GCNGC | 1 cut(s) | 230 |
| GsaI | CCCAGC | 2 cut(s) | 162, 198 |
| HaeII | RGCGCY | 1 cut(s) | 43 |
| HaeIII | GGCC | 2 cut(s) | 244, 330 |
| HapII | CCGG | 2 cut(s) | 205, 240 |
| HhaI | GCGC | 3 cut(s) | 42, 180, 319 |
| Hin1I | GRCGYC | 1 cut(s) | 40 |
| Hin1II | CATG | 1 cut(s) | 92 |
| Hin6I | GCGC | 3 cut(s) | 40, 178, 317 |
| HinP1I | GCGC | 3 cut(s) | 40, 178, 317 |
| HinfI | GANTC | 1 cut(s) | 4 |
| HpaII | CCGG | 2 cut(s) | 205, 240 |
| HphI | GGTGA | 2 cut(s) | 222, 245 |
| Hpy188III | TCNNGA | 1 cut(s) | 89 |
| HpyAV | CCTTC | 2 cut(s) | 294, 320 |
| HpyCH4III | ACNGT | 1 cut(s) | 99 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 175 |
| Hsp92I | GRCGYC | 1 cut(s) | 40 |
| Hsp92II | CATG | 1 cut(s) | 92 |
| HspAI | GCGC | 3 cut(s) | 40, 178, 317 |
| KasI | GGCGCC | 1 cut(s) | 39 |
| Kzo9I | GATC | 1 cut(s) | 248 |
| LguI | GCTCTTC | 1 cut(s) | 112 |
| LpnPI | CCDG | 9 cut(s) | 30, 108, 144, 172, 208, 218, 253, 255, 261 |
| Lsp1109I | GCAGC | 1 cut(s) | 241 |
| MaeI | CTAG | 1 cut(s) | 332 |
| MalI | GATC | 1 cut(s) | 250 |
| MbiI | CCGCTC | 1 cut(s) | 258 |
| MboI | GATC | 1 cut(s) | 248 |
| MboII | GAAGA | 1 cut(s) | 129 |
| MflI | RGATCY | 1 cut(s) | 248 |
| Mly113I | GGCGCC | 1 cut(s) | 40 |
| MnlI | CCTC | 1 cut(s) | 206 |
| MseI | TTAA | 2 cut(s) | 31, 172 |
| MspA1I | CMGCKG | 1 cut(s) | 162 |
| MspI | CCGG | 2 cut(s) | 205, 240 |
| MspR9I | CCNGG | 2 cut(s) | 205, 241 |
| MvnI | CGCG | 1 cut(s) | 178 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 175 |
| NarI | GGCGCC | 1 cut(s) | 40 |
| NciI | CCSGG | 2 cut(s) | 205, 241 |
| NdeII | GATC | 1 cut(s) | 248 |
| NlaIII | CATG | 1 cut(s) | 92 |
| NlaIV | GGNNCC | 1 cut(s) | 41 |
| NsbI | TGCGCA | 1 cut(s) | 318 |
| PagI | TCATGA | 1 cut(s) | 88 |
| PceI | AGGCCT | 1 cut(s) | 330 |
| PciSI | GCTCTTC | 1 cut(s) | 112 |
| PfeI | GAWTC | 1 cut(s) | 4 |
| PkrI | GCNGC | 1 cut(s) | 231 |
| PluTI | GGCGCC | 1 cut(s) | 43 |
| PspFI | CCCAGC | 2 cut(s) | 158, 194 |
| PspN4I | GGNNCC | 1 cut(s) | 41 |
| PspPI | GGNCC | 3 cut(s) | 128, 242, 281 |
| PsuI | RGATCY | 1 cut(s) | 248 |
| RsaI | GTAC | 1 cut(s) | 264 |
| RsaNI | GTAC | 1 cut(s) | 263 |
| SapI | GCTCTTC | 1 cut(s) | 112 |
| SaqAI | TTAA | 2 cut(s) | 31, 172 |
| SatI | GCNGC | 1 cut(s) | 230 |
| Sau3AI | GATC | 1 cut(s) | 248 |
| Sau96I | GGNCC | 3 cut(s) | 128, 242, 281 |
| ScrFI | CCNGG | 2 cut(s) | 205, 241 |
| SetI | ASST | 3 cut(s) | 71, 271, 286 |
| SfoI | GGCGCC | 1 cut(s) | 41 |
| SinI | GGWCC | 2 cut(s) | 128, 281 |
| SseBI | AGGCCT | 1 cut(s) | 330 |
| SsiI | CCGC | 9 cut(s) | 14, 103, 126, 136, 162, 176, 208, 256, 279 |
| SspDI | GGCGCC | 1 cut(s) | 39 |
| SspMI | CTAG | 1 cut(s) | 332 |
| StuI | AGGCCT | 1 cut(s) | 330 |
| StyD4I | CCNGG | 2 cut(s) | 203, 239 |
| StyI | CCWWGG | 1 cut(s) | 57 |
| TaaI | ACNGT | 1 cut(s) | 99 |
| TfiI | GAWTC | 1 cut(s) | 4 |
| Tru1I | TTAA | 2 cut(s) | 31, 172 |
| Tru9I | TTAA | 2 cut(s) | 31, 172 |
| TscAI | CASTG | 1 cut(s) | 280 |
| TseI | GCWGC | 1 cut(s) | 229 |
| TspRI | CASTG | 1 cut(s) | 280 |
| VpaK11BI | GGWCC | 2 cut(s) | 128, 281 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 288 |
| XspI | CTAG | 1 cut(s) | 332 |