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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 472 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 526 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 3 cut(s) | 74, 90, 219 |
| AclWI | GGATC | 5 cut(s) | 168, 201, 443, 631, 698 |
| AcoI | YGGCCR | 2 cut(s) | 108, 306 |
| AcuI | CTGAAG | 2 cut(s) | 48, 264 |
| AdeI | CACNNNGTG | 1 cut(s) | 329 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 462, 526, 692 |
| AgsI | TTSAA | 2 cut(s) | 424, 730 |
| AjnI | CCWGG | 5 cut(s) | 133, 156, 170, 347, 382 |
| AluBI | AGCT | 1 cut(s) | 43 |
| AluI | AGCT | 1 cut(s) | 43 |
| Alw21I | GWGCWC | 1 cut(s) | 45 |
| Alw26I | GTCTC | 1 cut(s) | 563 |
| AlwI | GGATC | 5 cut(s) | 168, 201, 443, 631, 698 |
| AoxI | GGCC | 8 cut(s) | 108, 154, 306, 386, 492, 619, 645, 653 |
| ApeKI | GCWGC | 5 cut(s) | 138, 141, 263, 342, 377 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 402 |
| AspA2I | CCTAGG | 1 cut(s) | 461 |
| AspLEI | GCGC | 7 cut(s) | 92, 219, 231, 268, 368, 382, 468 |
| AspS9I | GGNCC | 2 cut(s) | 386, 620 |
| AsuC2I | CCSGG | 6 cut(s) | 107, 117, 212, 320, 563, 618 |
| AsuHPI | GGTGA | 4 cut(s) | 134, 160, 458, 730 |
| AvrII | CCTAGG | 1 cut(s) | 461 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| Bbv12I | GWGCWC | 1 cut(s) | 45 |
| BbvI | GCAGC | 5 cut(s) | 150, 153, 250, 354, 389 |
| BccI | CCATC | 3 cut(s) | 59, 175, 385 |
| BceAI | ACGGC | 2 cut(s) | 617, 632 |
| BciT130I | CCWGG | 5 cut(s) | 135, 158, 172, 349, 384 |
| BcnI | CCSGG | 6 cut(s) | 107, 117, 212, 320, 563, 618 |
| BcoDI | GTCTC | 1 cut(s) | 563 |
| BfaI | CTAG | 1 cut(s) | 462 |
| BfmI | CTRYAG | 1 cut(s) | 641 |
| BfoI | RGCGCY | 2 cut(s) | 369, 383 |
| BfuAI | ACCTGC | 1 cut(s) | 472 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 378 |
| BlnI | CCTAGG | 1 cut(s) | 461 |
| BlsI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 344, 379 |
| BmgT120I | GGNCC | 2 cut(s) | 386, 620 |
| BmiI | GGNNCC | 2 cut(s) | 596, 622 |
| BmsI | GCATC | 2 cut(s) | 525, 547 |
| BpuMI | CCSGG | 6 cut(s) | 107, 117, 212, 320, 563, 618 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 115, 133, 318, 383, 461 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 462, 526, 692 |
| Bse118I | RCCGGY | 2 cut(s) | 368, 503 |
| Bse1I | ACTGG | 2 cut(s) | 192, 549 |
| BseBI | CCWGG | 5 cut(s) | 135, 158, 172, 349, 384 |
| BseDI | CCNNGG | 6 cut(s) | 105, 115, 133, 318, 383, 461 |
| BseGI | GGATG | 2 cut(s) | 51, 540 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 462, 526, 692 |
| BseMII | CTCAG | 2 cut(s) | 432, 491 |
| BseNI | ACTGG | 2 cut(s) | 192, 549 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 5 cut(s) | 150, 153, 250, 354, 389 |
| BseYI | CCCAGC | 2 cut(s) | 57, 362 |
| Bsh1236I | CGCG | 3 cut(s) | 74, 90, 219 |
| Bsh1285I | CGRYCG | 1 cut(s) | 78 |
| BshFI | GGCC | 8 cut(s) | 110, 156, 308, 388, 494, 621, 647, 655 |
| BsiEI | CGRYCG | 1 cut(s) | 78 |
| BsiHKAI | GWGCWC | 1 cut(s) | 45 |
| BsiSI | CCGG | 8 cut(s) | 107, 117, 211, 320, 369, 504, 562, 618 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 462, 526, 692 |
| BsmAI | GTCTC | 1 cut(s) | 563 |
| BsnI | GGCC | 8 cut(s) | 110, 156, 308, 388, 494, 621, 647, 655 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp143I | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| BspANI | GGCC | 8 cut(s) | 110, 156, 308, 388, 494, 621, 647, 655 |
| BspCNI | CTCAG | 2 cut(s) | 433, 490 |
| BspFNI | CGCG | 3 cut(s) | 74, 90, 219 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 2 cut(s) | 596, 622 |
| BspMI | ACCTGC | 1 cut(s) | 472 |
| BspPI | GGATC | 5 cut(s) | 168, 201, 443, 631, 698 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrFI | RCCGGY | 2 cut(s) | 368, 503 |
| BsrI | ACTGG | 2 cut(s) | 192, 549 |
| BssAI | RCCGGY | 2 cut(s) | 368, 503 |
| BssECI | CCNNGG | 6 cut(s) | 105, 115, 133, 318, 383, 461 |
| BssMI | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| BssT1I | CCWWGG | 1 cut(s) | 461 |
| Bst2UI | CCWGG | 5 cut(s) | 135, 158, 172, 349, 384 |
| Bst4CI | ACNGT | 1 cut(s) | 419 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 370, 411, 470, 505 |
| BstDEI | CTNAG | 2 cut(s) | 441, 477 |
| BstEII | GGTNACC | 1 cut(s) | 736 |
| BstF5I | GGATG | 2 cut(s) | 51, 540 |
| BstFNI | CGCG | 3 cut(s) | 74, 90, 219 |
| BstH2I | RGCGCY | 2 cut(s) | 369, 383 |
| BstHHI | GCGC | 7 cut(s) | 92, 219, 231, 268, 368, 382, 468 |
| BstKTI | GATC | 6 cut(s) | 78, 163, 196, 451, 639, 706 |
| BstMAI | GTCTC | 1 cut(s) | 563 |
| BstMBI | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| BstMCI | CGRYCG | 1 cut(s) | 78 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 40, 87, 144, 153, 374, 478, 500 |
| BstNI | CCWGG | 5 cut(s) | 135, 158, 172, 349, 384 |
| BstPI | GGTNACC | 1 cut(s) | 736 |
| BstSFI | CTRYAG | 1 cut(s) | 641 |
| BstUI | CGCG | 3 cut(s) | 74, 90, 219 |
| BstV1I | GCAGC | 5 cut(s) | 150, 153, 250, 354, 389 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 8 cut(s) | 110, 156, 308, 388, 494, 621, 647, 655 |
| BtsCI | GGATG | 2 cut(s) | 51, 540 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 472 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 370, 411, 470, 505 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 7 cut(s) | 92, 219, 231, 268, 368, 382, 468 |
| Cfr10I | RCCGGY | 2 cut(s) | 368, 503 |
| Cfr13I | GGNCC | 2 cut(s) | 386, 620 |
| CsiI | ACCWGGT | 1 cut(s) | 170 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 100, 135 |
| CviAII | CATG | 2 cut(s) | 271, 718 |
| CviQI | GTAC | 1 cut(s) | 175 |
| DdeI | CTNAG | 2 cut(s) | 441, 477 |
| DpnI | GATC | 6 cut(s) | 77, 162, 195, 450, 638, 705 |
| DpnII | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| DraIII | CACNNNGTG | 1 cut(s) | 329 |
| EaeI | YGGCCR | 2 cut(s) | 108, 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 8 cut(s) | 53, 206, 410, 441, 490, 581, 619, 708 |
| Ecl136II | GAGCTC | 1 cut(s) | 43 |
| Eco130I | CCWWGG | 1 cut(s) | 461 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco53kI | GAGCTC | 1 cut(s) | 43 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 264 |
| Eco91I | GGTNACC | 1 cut(s) | 736 |
| EcoICRI | GAGCTC | 1 cut(s) | 43 |
| EcoO65I | GGTNACC | 1 cut(s) | 736 |
| EcoRII | CCWGG | 5 cut(s) | 133, 156, 170, 347, 382 |
| EcoT14I | CCWWGG | 1 cut(s) | 461 |
| EcoT22I | ATGCAT | 1 cut(s) | 540 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| ErhI | CCWWGG | 1 cut(s) | 461 |
| FaeI | CATG | 2 cut(s) | 274, 721 |
| FaiI | YATR | 8 cut(s) | 201, 272, 458, 661, 663, 688, 690, 719 |
| FatI | CATG | 2 cut(s) | 270, 717 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| FauNDI | CATATG | 2 cut(s) | 661, 688 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 378 |
| FokI | GGATG | 2 cut(s) | 38, 547 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 378 |
| FspBI | CTAG | 1 cut(s) | 462 |
| GlaI | GCGC | 7 cut(s) | 91, 218, 230, 267, 367, 381, 467 |
| GluI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 378 |
| GsaI | CCCAGC | 2 cut(s) | 61, 366 |
| HaeII | RGCGCY | 2 cut(s) | 369, 383 |
| HaeIII | GGCC | 8 cut(s) | 110, 156, 308, 388, 494, 621, 647, 655 |
| HapII | CCGG | 8 cut(s) | 107, 117, 211, 320, 369, 504, 562, 618 |
| HhaI | GCGC | 7 cut(s) | 92, 219, 231, 268, 368, 382, 468 |
| Hin1II | CATG | 2 cut(s) | 274, 721 |
| Hin6I | GCGC | 7 cut(s) | 90, 217, 229, 266, 366, 380, 466 |
| HinP1I | GCGC | 7 cut(s) | 90, 217, 229, 266, 366, 380, 466 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 2 cut(s) | 398, 497 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 8 cut(s) | 107, 117, 211, 320, 369, 504, 562, 618 |
| HphI | GGTGA | 4 cut(s) | 134, 160, 458, 730 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 359, 606, 715 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 594 |
| Hpy188III | TCNNGA | 3 cut(s) | 271, 612, 730 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 359, 606, 715 |
| HpyAV | CCTTC | 3 cut(s) | 413, 727, 736 |
| HpyCH4III | ACNGT | 1 cut(s) | 419 |
| HpyCH4V | TGCA | 2 cut(s) | 538, 665 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 40, 87, 144, 153, 374, 478, 500 |
| HpyF3I | CTNAG | 2 cut(s) | 441, 477 |
| Hsp92II | CATG | 2 cut(s) | 274, 721 |
| HspAI | GCGC | 7 cut(s) | 90, 217, 229, 266, 366, 380, 466 |
| KroI | GCCGGC | 2 cut(s) | 368, 503 |
| KroNI | GCCGGC | 2 cut(s) | 370, 505 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 4 cut(s) | 40, 48, 214, 226 |
| Lsp1109I | GCAGC | 5 cut(s) | 150, 153, 250, 354, 389 |
| LweI | GCATC | 2 cut(s) | 525, 547 |
| MabI | ACCWGGT | 1 cut(s) | 170 |
| MaeI | CTAG | 1 cut(s) | 462 |
| MaeIII | GTNAC | 4 cut(s) | 166, 336, 697, 736 |
| MalI | GATC | 6 cut(s) | 77, 162, 195, 450, 638, 705 |
| MboI | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| MluCI | AATT | 2 cut(s) | 287, 521 |
| MmeI | TCCRAC | 2 cut(s) | 90, 605 |
| MnlI | CCTC | 8 cut(s) | 73, 118, 214, 217, 277, 436, 546, 609 |
| Mph1103I | ATGCAT | 1 cut(s) | 540 |
| MreI | CGCCGGCG | 1 cut(s) | 503 |
| MroNI | GCCGGC | 2 cut(s) | 368, 503 |
| MroXI | GAANNNNTTC | 1 cut(s) | 402 |
| MseI | TTAA | 3 cut(s) | 84, 290, 557 |
| MspA1I | CMGCKG | 1 cut(s) | 15 |
| MspI | CCGG | 8 cut(s) | 107, 117, 211, 320, 369, 504, 562, 618 |
| MvaI | CCWGG | 5 cut(s) | 135, 158, 172, 349, 384 |
| MvnI | CGCG | 3 cut(s) | 74, 90, 219 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 40, 87, 144, 153, 374, 478, 500 |
| NaeI | GCCGGC | 2 cut(s) | 370, 505 |
| NciI | CCSGG | 6 cut(s) | 107, 117, 212, 320, 563, 618 |
| NdeI | CATATG | 2 cut(s) | 661, 688 |
| NdeII | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| NgoMIV | GCCGGC | 2 cut(s) | 368, 503 |
| NlaIII | CATG | 2 cut(s) | 274, 721 |
| NlaIV | GGNNCC | 2 cut(s) | 596, 622 |
| NmuCI | GTSAC | 3 cut(s) | 166, 336, 736 |
| NsiI | ATGCAT | 1 cut(s) | 540 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 2 cut(s) | 370, 505 |
| PdmI | GAANNNNTTC | 1 cut(s) | 402 |
| PfeI | GAWTC | 2 cut(s) | 398, 497 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 526 |
| PkrI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 344, 379 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| Psp124BI | GAGCTC | 1 cut(s) | 45 |
| Psp6I | CCWGG | 5 cut(s) | 133, 156, 170, 347, 382 |
| PspEI | GGTNACC | 1 cut(s) | 736 |
| PspFI | CCCAGC | 2 cut(s) | 57, 362 |
| PspGI | CCWGG | 5 cut(s) | 133, 156, 170, 347, 382 |
| PspN4I | GGNNCC | 2 cut(s) | 596, 622 |
| PspPI | GGNCC | 2 cut(s) | 386, 620 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| SacI | GAGCTC | 1 cut(s) | 45 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 290, 557 |
| SatI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 378 |
| Sau3AI | GATC | 6 cut(s) | 75, 160, 193, 448, 636, 703 |
| Sau96I | GGNCC | 2 cut(s) | 386, 620 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SetI | ASST | 8 cut(s) | 45, 176, 261, 463, 486, 557, 728, 738 |
| SexAI | ACCWGGT | 1 cut(s) | 170 |
| SfaNI | GCATC | 2 cut(s) | 525, 547 |
| SfcI | CTRYAG | 1 cut(s) | 641 |
| SgrAI | CRCCGGYG | 1 cut(s) | 503 |
| Sse9I | AATT | 2 cut(s) | 287, 521 |
| SspMI | CTAG | 1 cut(s) | 462 |
| SstI | GAGCTC | 1 cut(s) | 45 |
| StyI | CCWWGG | 1 cut(s) | 461 |
| TaaI | ACNGT | 1 cut(s) | 419 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TasI | AATT | 2 cut(s) | 287, 521 |
| TauI | GCSGC | 2 cut(s) | 147, 308 |
| TfiI | GAWTC | 2 cut(s) | 398, 497 |
| Tru1I | TTAA | 3 cut(s) | 84, 290, 557 |
| Tru9I | TTAA | 3 cut(s) | 84, 290, 557 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 3 cut(s) | 166, 336, 736 |
| TseI | GCWGC | 5 cut(s) | 138, 141, 263, 342, 377 |
| Tsp45I | GTSAC | 3 cut(s) | 166, 336, 736 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 526 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 178 |
| XmaJI | CCTAGG | 1 cut(s) | 461 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 402 |
| XspI | CTAG | 1 cut(s) | 462 |
| Zsp2I | ATGCAT | 1 cut(s) | 540 |