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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 463 |
| Acc36I | ACCTGC | 2 cut(s) | 463, 556 |
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 4 cut(s) | 74, 266, 719, 1094 |
| AclI | AACGTT | 1 cut(s) | 936 |
| AcoI | YGGCCR | 4 cut(s) | 108, 261, 306, 1299 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 5 cut(s) | 660, 668, 965, 1143, 1151 |
| AfaI | GTAC | 6 cut(s) | 176, 396, 408, 1240, 1351, 1382 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 77, 368, 519, 546, 610, 1248, 1249, 1274 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AgsI | TTSAA | 4 cut(s) | 814, 906, 1000, 1377 |
| AjnI | CCWGG | 6 cut(s) | 347, 382, 421, 512, 892, 1258 |
| AleI | CACNNNNGTG | 1 cut(s) | 377 |
| AluBI | AGCT | 2 cut(s) | 607, 1316 |
| AluI | AGCT | 2 cut(s) | 607, 1316 |
| Alw26I | GTCTC | 3 cut(s) | 647, 1162, 1336 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 893 |
| ApeKI | GCWGC | 3 cut(s) | 141, 342, 1316 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 4 cut(s) | 231, 268, 368, 552 |
| AspS9I | GGNCC | 4 cut(s) | 386, 440, 704, 1106 |
| AsuC2I | CCSGG | 9 cut(s) | 107, 117, 320, 647, 665, 702, 1110, 1147, 1165 |
| AsuHPI | GGTGA | 2 cut(s) | 542, 1000 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BbvI | GCAGC | 3 cut(s) | 153, 354, 1303 |
| BccI | CCATC | 1 cut(s) | 253 |
| BceAI | ACGGC | 3 cut(s) | 35, 913, 1374 |
| BciT130I | CCWGG | 6 cut(s) | 349, 384, 423, 514, 894, 1260 |
| BcnI | CCSGG | 9 cut(s) | 107, 117, 320, 647, 665, 702, 1110, 1147, 1165 |
| BcoDI | GTCTC | 3 cut(s) | 647, 1162, 1336 |
| BfaI | CTAG | 1 cut(s) | 546 |
| BfmI | CTRYAG | 2 cut(s) | 725, 1084 |
| BfoI | RGCGCY | 1 cut(s) | 369 |
| BfuAI | ACCTGC | 2 cut(s) | 463, 556 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 562 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 9 cut(s) | 142, 264, 306, 343, 446, 717, 1095, 1299, 1317 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 9 cut(s) | 143, 265, 307, 344, 447, 718, 1096, 1300, 1318 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmgT120I | GGNCC | 4 cut(s) | 386, 440, 704, 1106 |
| BmiI | GGNNCC | 6 cut(s) | 381, 441, 680, 706, 1107, 1133 |
| BmsI | GCATC | 2 cut(s) | 583, 609 |
| BoxI | GACNNNNGTC | 2 cut(s) | 659, 1154 |
| BpmI | CTGGAG | 2 cut(s) | 650, 1165 |
| BpuMI | CCSGG | 9 cut(s) | 107, 117, 320, 647, 665, 702, 1110, 1147, 1165 |
| BsaHI | GRCGYC | 5 cut(s) | 660, 668, 965, 1143, 1151 |
| BsaJI | CCNNGG | 7 cut(s) | 105, 115, 383, 422, 513, 545, 1258 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 623, 653, 1163, 1193 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 77, 368, 519, 546, 610, 1248, 1249, 1274 |
| Bse118I | RCCGGY | 4 cut(s) | 368, 388, 587, 1267 |
| Bse1I | ACTGG | 6 cut(s) | 134, 192, 633, 913, 1182, 1396 |
| Bse3DI | GCAATG | 2 cut(s) | 918, 1326 |
| BseBI | CCWGG | 6 cut(s) | 349, 384, 423, 514, 894, 1260 |
| BseDI | CCNNGG | 7 cut(s) | 105, 115, 383, 422, 513, 545, 1258 |
| BseGI | GGATG | 1 cut(s) | 624 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 77, 368, 519, 546, 610, 1248, 1249, 1274 |
| BseMI | GCAATG | 2 cut(s) | 918, 1326 |
| BseMII | CTCAG | 1 cut(s) | 516 |
| BseNI | ACTGG | 6 cut(s) | 134, 192, 633, 913, 1182, 1396 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 3 cut(s) | 153, 354, 1303 |
| BseYI | CCCAGC | 2 cut(s) | 362, 603 |
| Bsh1236I | CGCG | 4 cut(s) | 74, 266, 719, 1094 |
| Bsh1285I | CGRYCG | 4 cut(s) | 149, 316, 393, 827 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 4 cut(s) | 149, 316, 393, 827 |
| BslFI | GGGAC | 4 cut(s) | 38, 453, 647, 1162 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 77, 368, 519, 546, 610, 1248, 1249, 1274 |
| BsmAI | GTCTC | 3 cut(s) | 647, 1162, 1336 |
| BsmBI | CGTCTC | 2 cut(s) | 647, 1162 |
| BsmFI | GGGAC | 4 cut(s) | 38, 453, 647, 1162 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| Bsp1407I | TGTACA | 1 cut(s) | 1349 |
| BspCNI | CTCAG | 1 cut(s) | 517 |
| BspFNI | CGCG | 4 cut(s) | 74, 266, 719, 1094 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 6 cut(s) | 381, 441, 680, 706, 1107, 1133 |
| BspMAI | CTGCAG | 2 cut(s) | 729, 1088 |
| BspMI | ACCTGC | 2 cut(s) | 463, 556 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrDI | GCAATG | 2 cut(s) | 918, 1326 |
| BsrFI | RCCGGY | 4 cut(s) | 368, 388, 587, 1267 |
| BsrGI | TGTACA | 1 cut(s) | 1349 |
| BsrI | ACTGG | 6 cut(s) | 134, 192, 633, 913, 1182, 1396 |
| BssAI | RCCGGY | 4 cut(s) | 368, 388, 587, 1267 |
| BssECI | CCNNGG | 7 cut(s) | 105, 115, 383, 422, 513, 545, 1258 |
| BssNI | GRCGYC | 5 cut(s) | 660, 668, 965, 1143, 1151 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 6 cut(s) | 349, 384, 423, 514, 894, 1260 |
| Bst4CI | ACNGT | 3 cut(s) | 150, 869, 1354 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 5 cut(s) | 660, 668, 965, 1143, 1151 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1277 |
| BstAUI | TGTACA | 1 cut(s) | 1349 |
| BstC8I | GCNNGC | 7 cut(s) | 36, 332, 370, 554, 729, 862, 1084 |
| BstDEI | CTNAG | 1 cut(s) | 525 |
| BstEII | GGTNACC | 1 cut(s) | 988 |
| BstF5I | GGATG | 1 cut(s) | 624 |
| BstFNI | CGCG | 4 cut(s) | 74, 266, 719, 1094 |
| BstH2I | RGCGCY | 1 cut(s) | 369 |
| BstHHI | GCGC | 4 cut(s) | 231, 268, 368, 552 |
| BstMAI | GTCTC | 3 cut(s) | 647, 1162, 1336 |
| BstMCI | CGRYCG | 4 cut(s) | 149, 316, 393, 827 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 87, 409, 562, 1277 |
| BstNI | CCWGG | 6 cut(s) | 349, 384, 423, 514, 894, 1260 |
| BstNSI | RCATGY | 2 cut(s) | 407, 864 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 659, 1154 |
| BstPI | GGTNACC | 1 cut(s) | 988 |
| BstSFI | CTRYAG | 2 cut(s) | 725, 1084 |
| BstUI | CGCG | 4 cut(s) | 74, 266, 719, 1094 |
| BstV1I | GCAGC | 3 cut(s) | 153, 354, 1303 |
| BstX2I | RGATCY | 4 cut(s) | 160, 193, 889, 1214 |
| BstYI | RGATCY | 4 cut(s) | 160, 193, 889, 1214 |
| BtgZI | GCGATG | 2 cut(s) | 135, 606 |
| BtsCI | GGATG | 1 cut(s) | 624 |
| BtsIMutI | CAGTG | 4 cut(s) | 185, 874, 1207, 1359 |
| BveI | ACCTGC | 2 cut(s) | 463, 556 |
| Cac8I | GCNNGC | 7 cut(s) | 36, 332, 370, 554, 729, 862, 1084 |
| CaiI | CAGNNNCTG | 1 cut(s) | 893 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 4 cut(s) | 231, 268, 368, 552 |
| Cfr10I | RCCGGY | 4 cut(s) | 368, 388, 587, 1267 |
| Cfr13I | GGNCC | 4 cut(s) | 386, 440, 704, 1106 |
| CseI | GACGC | 5 cut(s) | 649, 657, 954, 1151, 1159 |
| Csp6I | GTAC | 6 cut(s) | 175, 395, 407, 1239, 1350, 1381 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 7 cut(s) | 172, 271, 404, 802, 861, 873, 1009 |
| CviQI | GTAC | 6 cut(s) | 175, 395, 407, 1239, 1350, 1381 |
| DdeI | CTNAG | 1 cut(s) | 525 |
| EaeI | YGGCCR | 4 cut(s) | 108, 261, 306, 1299 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco91I | GGTNACC | 1 cut(s) | 988 |
| EcoO65I | GGTNACC | 1 cut(s) | 988 |
| EcoRII | CCWGG | 6 cut(s) | 347, 382, 421, 512, 892, 1258 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| Esp3I | CGTCTC | 2 cut(s) | 647, 1162 |
| FaeI | CATG | 7 cut(s) | 175, 274, 407, 805, 864, 876, 1012 |
| FaqI | GGGAC | 4 cut(s) | 38, 453, 647, 1162 |
| FatI | CATG | 7 cut(s) | 171, 270, 403, 801, 860, 872, 1008 |
| FauI | CCCGC | 5 cut(s) | 8, 79, 690, 840, 1121 |
| FauNDI | CATATG | 2 cut(s) | 745, 1066 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 9 cut(s) | 142, 264, 306, 343, 446, 717, 1095, 1299, 1317 |
| FokI | GGATG | 1 cut(s) | 631 |
| Fsp4HI | GCNGC | 9 cut(s) | 142, 264, 306, 343, 446, 717, 1095, 1299, 1317 |
| FspBI | CTAG | 1 cut(s) | 546 |
| GlaI | GCGC | 4 cut(s) | 230, 267, 367, 551 |
| GluI | GCNGC | 9 cut(s) | 142, 264, 306, 343, 446, 717, 1095, 1299, 1317 |
| GsaI | CCCAGC | 2 cut(s) | 366, 607 |
| GsuI | CTGGAG | 2 cut(s) | 650, 1165 |
| HaeII | RGCGCY | 1 cut(s) | 369 |
| HgaI | GACGC | 5 cut(s) | 649, 657, 954, 1151, 1159 |
| HhaI | GCGC | 4 cut(s) | 231, 268, 368, 552 |
| Hin1I | GRCGYC | 5 cut(s) | 660, 668, 965, 1143, 1151 |
| Hin1II | CATG | 7 cut(s) | 175, 274, 407, 805, 864, 876, 1012 |
| Hin6I | GCGC | 4 cut(s) | 229, 266, 366, 550 |
| HinP1I | GCGC | 4 cut(s) | 229, 266, 366, 550 |
| HincII | GTYRAC | 4 cut(s) | 85, 359, 672, 1141 |
| HindII | GTYRAC | 4 cut(s) | 85, 359, 672, 1141 |
| HinfI | GANTC | 3 cut(s) | 482, 581, 973 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HphI | GGTGA | 2 cut(s) | 542, 1000 |
| Hpy166II | GTNNAC | 7 cut(s) | 85, 359, 672, 799, 1014, 1141, 1255 |
| Hpy188I | TCNGA | 6 cut(s) | 67, 678, 737, 1077, 1136, 1214 |
| Hpy188III | TCNNGA | 7 cut(s) | 100, 271, 432, 696, 814, 997, 1115 |
| Hpy8I | GTNNAC | 7 cut(s) | 85, 359, 672, 799, 1014, 1141, 1255 |
| Hpy99I | CGWCG | 2 cut(s) | 970, 973 |
| HpyAV | CCTTC | 5 cut(s) | 232, 811, 820, 994, 1003 |
| HpyCH4III | ACNGT | 3 cut(s) | 150, 869, 1354 |
| HpyCH4IV | ACGT | 2 cut(s) | 277, 936 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 87, 409, 562, 1277 |
| HpyF3I | CTNAG | 1 cut(s) | 525 |
| HpySE526I | ACGT | 2 cut(s) | 277, 936 |
| Hsp92I | GRCGYC | 5 cut(s) | 660, 668, 965, 1143, 1151 |
| Hsp92II | CATG | 7 cut(s) | 175, 274, 407, 805, 864, 876, 1012 |
| HspAI | GCGC | 4 cut(s) | 229, 266, 366, 550 |
| KroI | GCCGGC | 1 cut(s) | 368 |
| KroNI | GCCGGC | 1 cut(s) | 370 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 4 cut(s) | 226, 631, 1184, 1368 |
| Lsp1109I | GCAGC | 3 cut(s) | 153, 354, 1303 |
| LweI | GCATC | 2 cut(s) | 583, 609 |
| MaeI | CTAG | 1 cut(s) | 546 |
| MaeII | ACGT | 2 cut(s) | 277, 936 |
| MaeIII | GTNAC | 3 cut(s) | 336, 988, 1354 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 4 cut(s) | 160, 193, 889, 1214 |
| MmeI | TCCRAC | 4 cut(s) | 90, 957, 1166, 1346 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MroNI | GCCGGC | 1 cut(s) | 368 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 2 cut(s) | 170, 377 |
| MspA1I | CMGCKG | 3 cut(s) | 412, 1298, 1316 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 6 cut(s) | 349, 384, 423, 514, 894, 1260 |
| MvnI | CGCG | 4 cut(s) | 74, 266, 719, 1094 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 87, 409, 562, 1277 |
| NaeI | GCCGGC | 1 cut(s) | 370 |
| NciI | CCSGG | 9 cut(s) | 107, 117, 320, 647, 665, 702, 1110, 1147, 1165 |
| NdeI | CATATG | 2 cut(s) | 745, 1066 |
| NgoMIV | GCCGGC | 1 cut(s) | 368 |
| NlaIII | CATG | 7 cut(s) | 175, 274, 407, 805, 864, 876, 1012 |
| NlaIV | GGNNCC | 6 cut(s) | 381, 441, 680, 706, 1107, 1133 |
| NmeAIII | GCCGAG | 1 cut(s) | 541 |
| NmuCI | GTSAC | 3 cut(s) | 336, 988, 1354 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 2 cut(s) | 407, 864 |
| OliI | CACNNNNGTG | 1 cut(s) | 377 |
| PaeI | GCATGC | 1 cut(s) | 864 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PaqCI | CACCTGC | 1 cut(s) | 463 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdiI | GCCGGC | 1 cut(s) | 370 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 3 cut(s) | 482, 581, 973 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 9 cut(s) | 143, 265, 307, 344, 447, 718, 1096, 1300, 1318 |
| PshAI | GACNNNNGTC | 2 cut(s) | 659, 1154 |
| Psp1406I | AACGTT | 1 cut(s) | 936 |
| Psp6I | CCWGG | 6 cut(s) | 347, 382, 421, 512, 892, 1258 |
| PspEI | GGTNACC | 1 cut(s) | 988 |
| PspFI | CCCAGC | 2 cut(s) | 362, 603 |
| PspGI | CCWGG | 6 cut(s) | 347, 382, 421, 512, 892, 1258 |
| PspN4I | GGNNCC | 6 cut(s) | 381, 441, 680, 706, 1107, 1133 |
| PspPI | GGNCC | 4 cut(s) | 386, 440, 704, 1106 |
| PstI | CTGCAG | 2 cut(s) | 729, 1088 |
| PstNI | CAGNNNCTG | 1 cut(s) | 893 |
| PsuI | RGATCY | 4 cut(s) | 160, 193, 889, 1214 |
| PvuII | CAGCTG | 1 cut(s) | 1316 |
| RsaI | GTAC | 6 cut(s) | 176, 396, 408, 1240, 1351, 1382 |
| RsaNI | GTAC | 6 cut(s) | 175, 395, 407, 1239, 1350, 1381 |
| RseI | CAYNNNNRTG | 2 cut(s) | 170, 377 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 9 cut(s) | 142, 264, 306, 343, 446, 717, 1095, 1299, 1317 |
| Sau96I | GGNCC | 4 cut(s) | 386, 440, 704, 1106 |
| SbfI | CCTGCAGG | 2 cut(s) | 729, 1088 |
| SdaI | CCTGCAGG | 2 cut(s) | 729, 1088 |
| SfaNI | GCATC | 2 cut(s) | 583, 609 |
| SfcI | CTRYAG | 2 cut(s) | 725, 1084 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 170, 377 |
| SphI | GCATGC | 1 cut(s) | 864 |
| Sse8387I | CCTGCAGG | 2 cut(s) | 729, 1088 |
| SspMI | CTAG | 1 cut(s) | 546 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaaI | ACNGT | 3 cut(s) | 150, 869, 1354 |
| TaiI | ACGT | 2 cut(s) | 280, 939 |
| TaqI | TCGA | 3 cut(s) | 358, 506, 971 |
| TaqII | GACCGA | 5 cut(s) | 754, 800, 813, 1015, 1061 |
| TatI | WGTACW | 1 cut(s) | 1349 |
| TauI | GCSGC | 6 cut(s) | 266, 308, 448, 719, 1097, 1301 |
| TfiI | GAWTC | 3 cut(s) | 482, 581, 973 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 4 cut(s) | 192, 874, 1207, 1359 |
| TseFI | GTSAC | 3 cut(s) | 336, 988, 1354 |
| TseI | GCWGC | 3 cut(s) | 141, 342, 1316 |
| Tsp45I | GTSAC | 3 cut(s) | 336, 988, 1354 |
| TspDTI | ATGAA | 3 cut(s) | 188, 857, 907 |
| TspRI | CASTG | 4 cut(s) | 192, 874, 1207, 1359 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XceI | RCATGY | 2 cut(s) | 407, 864 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 1 cut(s) | 546 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |