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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 368 |
| Acc65I | GGTACC | 1 cut(s) | 268 |
| AccB1I | GGYRCC | 1 cut(s) | 268 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 435 |
| AccI | GTMKAC | 1 cut(s) | 247 |
| AciI | CCGC | 9 cut(s) | 9, 34, 38, 304, 316, 327, 361, 390, 410 |
| AcoI | YGGCCR | 1 cut(s) | 206 |
| AcuI | CTGAAG | 1 cut(s) | 371 |
| AfaI | GTAC | 2 cut(s) | 65, 270 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 40, 145, 204, 257, 333, 435 |
| AflIII | ACRYGT | 1 cut(s) | 164 |
| AjnI | CCWGG | 3 cut(s) | 4, 236, 271 |
| AluBI | AGCT | 1 cut(s) | 70 |
| AluI | AGCT | 1 cut(s) | 70 |
| AoxI | GGCC | 5 cut(s) | 43, 135, 195, 206, 275 |
| ApeKI | GCWGC | 2 cut(s) | 27, 30 |
| Asp718I | GGTACC | 1 cut(s) | 268 |
| AspLEI | GCGC | 2 cut(s) | 120, 377 |
| AspS9I | GGNCC | 3 cut(s) | 43, 136, 275 |
| AsuC2I | CCSGG | 3 cut(s) | 42, 252, 387 |
| AsuHPI | GGTGA | 1 cut(s) | 23 |
| AsuNHI | GCTAGC | 1 cut(s) | 70 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 252, 285 |
| BanI | GGYRCC | 1 cut(s) | 268 |
| BbsI | GAAGAC | 1 cut(s) | 191 |
| BbvCI | CCTCAGC | 1 cut(s) | 132 |
| BbvI | GCAGC | 2 cut(s) | 39, 42 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 406, 440 |
| BciT130I | CCWGG | 3 cut(s) | 6, 238, 273 |
| BcnI | CCSGG | 3 cut(s) | 42, 252, 387 |
| BfaI | CTAG | 4 cut(s) | 71, 191, 330, 338 |
| BfuAI | ACCTGC | 1 cut(s) | 368 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 42 |
| BglII | AGATCT | 1 cut(s) | 49 |
| BisI | GCNGC | 3 cut(s) | 28, 31, 34 |
| BlsI | GCNGC | 3 cut(s) | 29, 32, 35 |
| Bme1390I | CCNGG | 6 cut(s) | 6, 42, 238, 252, 273, 387 |
| BmgT120I | GGNCC | 3 cut(s) | 43, 136, 275 |
| BmiI | GGNNCC | 1 cut(s) | 270 |
| BmrFI | CCNGG | 6 cut(s) | 6, 42, 238, 252, 273, 387 |
| BmsI | GCATC | 2 cut(s) | 279, 408 |
| BmtI | GCTAGC | 1 cut(s) | 74 |
| BpiI | GAAGAC | 1 cut(s) | 191 |
| Bpu10I | CCTNAGC | 1 cut(s) | 132 |
| BpuMI | CCSGG | 3 cut(s) | 42, 252, 387 |
| BsaAI | YACGTR | 1 cut(s) | 230 |
| BsaJI | CCNNGG | 3 cut(s) | 4, 272, 370 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 448 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 40, 145, 204, 257, 333, 435 |
| Bse118I | RCCGGY | 2 cut(s) | 204, 277 |
| Bse1I | ACTGG | 3 cut(s) | 81, 262, 458 |
| BseBI | CCWGG | 3 cut(s) | 6, 238, 273 |
| BseDI | CCNNGG | 3 cut(s) | 4, 272, 370 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 40, 145, 204, 257, 333, 435 |
| BseMII | CTCAG | 1 cut(s) | 123 |
| BseNI | ACTGG | 3 cut(s) | 81, 262, 458 |
| BseXI | GCAGC | 2 cut(s) | 39, 42 |
| BsgI | GTGCAG | 1 cut(s) | 305 |
| Bsh1285I | CGRYCG | 1 cut(s) | 205 |
| BshFI | GGCC | 5 cut(s) | 45, 137, 197, 208, 277 |
| BshNI | GGYRCC | 1 cut(s) | 268 |
| BsiEI | CGRYCG | 1 cut(s) | 205 |
| BsiSI | CCGG | 5 cut(s) | 41, 205, 252, 278, 387 |
| BslFI | GGGAC | 1 cut(s) | 369 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 40, 145, 204, 257, 333, 435 |
| BsmFI | GGGAC | 1 cut(s) | 369 |
| BsmI | GAATGC | 1 cut(s) | 449 |
| BsnI | GGCC | 5 cut(s) | 45, 137, 197, 208, 277 |
| Bsp143I | GATC | 1 cut(s) | 49 |
| BspACI | CCGC | 9 cut(s) | 9, 34, 38, 304, 316, 327, 361, 390, 410 |
| BspANI | GGCC | 5 cut(s) | 45, 137, 197, 208, 277 |
| BspCNI | CTCAG | 1 cut(s) | 124 |
| BspHI | TCATGA | 1 cut(s) | 159 |
| BspLI | GGNNCC | 1 cut(s) | 270 |
| BspMI | ACCTGC | 1 cut(s) | 368 |
| BspOI | GCTAGC | 1 cut(s) | 74 |
| BspT107I | GGYRCC | 1 cut(s) | 268 |
| BsrFI | RCCGGY | 2 cut(s) | 204, 277 |
| BsrI | ACTGG | 3 cut(s) | 81, 262, 458 |
| BssAI | RCCGGY | 2 cut(s) | 204, 277 |
| BssECI | CCNNGG | 3 cut(s) | 4, 272, 370 |
| BssMI | GATC | 1 cut(s) | 49 |
| BssT1I | CCWWGG | 1 cut(s) | 370 |
| Bst2UI | CCWGG | 3 cut(s) | 6, 238, 273 |
| BstBAI | YACGTR | 1 cut(s) | 230 |
| BstC8I | GCNNGC | 4 cut(s) | 38, 72, 210, 221 |
| BstDEI | CTNAG | 1 cut(s) | 132 |
| BstHHI | GCGC | 2 cut(s) | 120, 377 |
| BstKTI | GATC | 1 cut(s) | 52 |
| BstMBI | GATC | 1 cut(s) | 49 |
| BstMCI | CGRYCG | 1 cut(s) | 205 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 33, 42, 283, 409, 418 |
| BstNI | CCWGG | 3 cut(s) | 6, 238, 273 |
| BstSCI | CCNGG | 6 cut(s) | 4, 40, 236, 250, 271, 385 |
| BstV1I | GCAGC | 2 cut(s) | 39, 42 |
| BstV2I | GAAGAC | 1 cut(s) | 191 |
| BstX2I | RGATCY | 1 cut(s) | 49 |
| BstYI | RGATCY | 1 cut(s) | 49 |
| BsuRI | GGCC | 5 cut(s) | 45, 137, 197, 208, 277 |
| BtsIMutI | CAGTG | 2 cut(s) | 74, 255 |
| BveI | ACCTGC | 1 cut(s) | 368 |
| Cac8I | GCNNGC | 4 cut(s) | 38, 72, 210, 221 |
| CciI | TCATGA | 1 cut(s) | 159 |
| CfoI | GCGC | 2 cut(s) | 120, 377 |
| Cfr10I | RCCGGY | 2 cut(s) | 204, 277 |
| Cfr13I | GGNCC | 3 cut(s) | 43, 136, 275 |
| CseI | GACGC | 1 cut(s) | 196 |
| Csp6I | GTAC | 2 cut(s) | 64, 269 |
| CviAII | CATG | 1 cut(s) | 160 |
| CviQI | GTAC | 2 cut(s) | 64, 269 |
| DdeI | CTNAG | 1 cut(s) | 132 |
| DpnI | GATC | 1 cut(s) | 51 |
| DpnII | GATC | 1 cut(s) | 49 |
| EaeI | YGGCCR | 1 cut(s) | 206 |
| EciI | GGCGGA | 3 cut(s) | 319, 350, 399 |
| Eco130I | CCWWGG | 1 cut(s) | 370 |
| Eco57I | CTGAAG | 1 cut(s) | 371 |
| EcoRII | CCWGG | 3 cut(s) | 4, 236, 271 |
| EcoT14I | CCWWGG | 1 cut(s) | 370 |
| ErhI | CCWWGG | 1 cut(s) | 370 |
| FaeI | CATG | 1 cut(s) | 163 |
| FaiI | YATR | 3 cut(s) | 62, 90, 161 |
| FaqI | GGGAC | 1 cut(s) | 369 |
| FatI | CATG | 1 cut(s) | 159 |
| FauI | CCCGC | 3 cut(s) | 45, 323, 383 |
| FblI | GTMKAC | 1 cut(s) | 247 |
| Fnu4HI | GCNGC | 3 cut(s) | 28, 31, 34 |
| Fsp4HI | GCNGC | 3 cut(s) | 28, 31, 34 |
| FspBI | CTAG | 4 cut(s) | 71, 191, 330, 338 |
| GlaI | GCGC | 2 cut(s) | 119, 376 |
| GluI | GCNGC | 3 cut(s) | 28, 31, 34 |
| HaeIII | GGCC | 5 cut(s) | 45, 137, 197, 208, 277 |
| HapII | CCGG | 5 cut(s) | 41, 205, 252, 278, 387 |
| HgaI | GACGC | 1 cut(s) | 196 |
| HhaI | GCGC | 2 cut(s) | 120, 377 |
| Hin1II | CATG | 1 cut(s) | 163 |
| Hin6I | GCGC | 2 cut(s) | 118, 375 |
| HinP1I | GCGC | 2 cut(s) | 118, 375 |
| HincII | GTYRAC | 1 cut(s) | 248 |
| HindII | GTYRAC | 1 cut(s) | 248 |
| HinfI | GANTC | 3 cut(s) | 82, 307, 406 |
| HpaII | CCGG | 5 cut(s) | 41, 205, 252, 278, 387 |
| HphI | GGTGA | 1 cut(s) | 23 |
| Hpy166II | GTNNAC | 1 cut(s) | 248 |
| Hpy188III | TCNNGA | 1 cut(s) | 160 |
| Hpy8I | GTNNAC | 1 cut(s) | 248 |
| Hpy99I | CGWCG | 1 cut(s) | 418 |
| HpyAV | CCTTC | 2 cut(s) | 106, 346 |
| HpyCH4IV | ACGT | 3 cut(s) | 106, 166, 229 |
| HpyCH4V | TGCA | 4 cut(s) | 286, 292, 421, 447 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 33, 42, 283, 409, 418 |
| HpyF3I | CTNAG | 1 cut(s) | 132 |
| HpySE526I | ACGT | 3 cut(s) | 106, 166, 229 |
| Hsp92II | CATG | 1 cut(s) | 163 |
| HspAI | GCGC | 2 cut(s) | 118, 375 |
| KpnI | GGTACC | 1 cut(s) | 272 |
| Kzo9I | GATC | 1 cut(s) | 49 |
| LmnI | GCTCC | 1 cut(s) | 115 |
| Lsp1109I | GCAGC | 2 cut(s) | 39, 42 |
| LweI | GCATC | 2 cut(s) | 279, 408 |
| MaeI | CTAG | 4 cut(s) | 71, 191, 330, 338 |
| MaeII | ACGT | 3 cut(s) | 106, 166, 229 |
| MaeIII | GTNAC | 2 cut(s) | 55, 225 |
| MalI | GATC | 1 cut(s) | 51 |
| MboI | GATC | 1 cut(s) | 49 |
| MboII | GAAGA | 2 cut(s) | 77, 196 |
| MflI | RGATCY | 1 cut(s) | 49 |
| MmeI | TCCRAC | 1 cut(s) | 416 |
| MnlI | CCTC | 7 cut(s) | 7, 127, 166, 187, 294, 327, 455 |
| MspI | CCGG | 5 cut(s) | 41, 205, 252, 278, 387 |
| MspR9I | CCNGG | 6 cut(s) | 6, 42, 238, 252, 273, 387 |
| Mva1269I | GAATGC | 1 cut(s) | 449 |
| MvaI | CCWGG | 3 cut(s) | 6, 238, 273 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 33, 42, 283, 409, 418 |
| NciI | CCSGG | 3 cut(s) | 42, 252, 387 |
| NdeII | GATC | 1 cut(s) | 49 |
| NheI | GCTAGC | 1 cut(s) | 70 |
| NlaIII | CATG | 1 cut(s) | 163 |
| NlaIV | GGNNCC | 1 cut(s) | 270 |
| NmuCI | GTSAC | 2 cut(s) | 55, 225 |
| PagI | TCATGA | 1 cut(s) | 159 |
| PctI | GAATGC | 1 cut(s) | 449 |
| PfeI | GAWTC | 3 cut(s) | 82, 307, 406 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 435 |
| PkrI | GCNGC | 3 cut(s) | 29, 32, 35 |
| Ppu21I | YACGTR | 1 cut(s) | 230 |
| Psp6I | CCWGG | 3 cut(s) | 4, 236, 271 |
| PspGI | CCWGG | 3 cut(s) | 4, 236, 271 |
| PspN4I | GGNNCC | 1 cut(s) | 270 |
| PspPI | GGNCC | 3 cut(s) | 43, 136, 275 |
| PsuI | RGATCY | 1 cut(s) | 49 |
| RsaI | GTAC | 2 cut(s) | 65, 270 |
| RsaNI | GTAC | 2 cut(s) | 64, 269 |
| SalI | GTCGAC | 1 cut(s) | 246 |
| SatI | GCNGC | 3 cut(s) | 28, 31, 34 |
| Sau3AI | GATC | 1 cut(s) | 49 |
| Sau96I | GGNCC | 3 cut(s) | 43, 136, 275 |
| ScrFI | CCNGG | 6 cut(s) | 6, 42, 238, 252, 273, 387 |
| SetI | ASST | 7 cut(s) | 72, 109, 150, 169, 232, 382, 466 |
| SfaNI | GCATC | 2 cut(s) | 279, 408 |
| SsiI | CCGC | 9 cut(s) | 9, 34, 38, 304, 316, 327, 361, 390, 410 |
| SspMI | CTAG | 4 cut(s) | 71, 191, 330, 338 |
| StyD4I | CCNGG | 6 cut(s) | 4, 40, 236, 250, 271, 385 |
| StyI | CCWWGG | 1 cut(s) | 370 |
| TaiI | ACGT | 3 cut(s) | 109, 169, 232 |
| TaqI | TCGA | 1 cut(s) | 247 |
| TauI | GCSGC | 1 cut(s) | 36 |
| TfiI | GAWTC | 3 cut(s) | 82, 307, 406 |
| TscAI | CASTG | 2 cut(s) | 81, 262 |
| TseFI | GTSAC | 2 cut(s) | 55, 225 |
| TseI | GCWGC | 2 cut(s) | 27, 30 |
| Tsp45I | GTSAC | 2 cut(s) | 55, 225 |
| TspDTI | ATGAA | 1 cut(s) | 77 |
| TspRI | CASTG | 2 cut(s) | 81, 262 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 435 |
| XmiI | GTMKAC | 1 cut(s) | 247 |
| XspI | CTAG | 4 cut(s) | 71, 191, 330, 338 |