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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 446 |
| AccII | CGCG | 4 cut(s) | 90, 92, 122, 150 |
| AciI | CCGC | 8 cut(s) | 15, 48, 88, 122, 429, 444, 454, 483 |
| AcoI | YGGCCR | 1 cut(s) | 192 |
| AfeI | AGCGCT | 1 cut(s) | 494 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 121, 299 |
| AgsI | TTSAA | 1 cut(s) | 341 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 296 |
| AjnI | CCWGG | 1 cut(s) | 278 |
| AleI | CACNNNNGTG | 1 cut(s) | 200 |
| AluBI | AGCT | 1 cut(s) | 220 |
| AluI | AGCT | 1 cut(s) | 220 |
| Alw21I | GWGCWC | 1 cut(s) | 278 |
| Alw26I | GTCTC | 1 cut(s) | 467 |
| Aor51HI | AGCGCT | 1 cut(s) | 494 |
| AoxI | GGCC | 3 cut(s) | 171, 177, 192 |
| AspLEI | GCGC | 6 cut(s) | 92, 94, 122, 150, 473, 495 |
| AspS9I | GGNCC | 2 cut(s) | 124, 178 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 251 |
| AsuHPI | GGTGA | 1 cut(s) | 91 |
| AvaII | GGWCC | 1 cut(s) | 124 |
| BalI | TGGCCA | 1 cut(s) | 194 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| Bbv12I | GWGCWC | 1 cut(s) | 278 |
| BccI | CCATC | 2 cut(s) | 364, 482 |
| BciT130I | CCWGG | 1 cut(s) | 280 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 251 |
| BcoDI | GTCTC | 1 cut(s) | 467 |
| BfaI | CTAG | 3 cut(s) | 233, 294, 383 |
| BfoI | RGCGCY | 2 cut(s) | 474, 496 |
| Bme1390I | CCNGG | 4 cut(s) | 107, 117, 251, 280 |
| Bme18I | GGWCC | 1 cut(s) | 124 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 296 |
| BmgT120I | GGNCC | 2 cut(s) | 124, 178 |
| BmrFI | CCNGG | 4 cut(s) | 107, 117, 251, 280 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 1 cut(s) | 451 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 251 |
| BsaJI | CCNNGG | 3 cut(s) | 105, 115, 174 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 449, 479 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 121, 299 |
| Bse1I | ACTGG | 1 cut(s) | 352 |
| Bse3DI | GCAATG | 1 cut(s) | 305 |
| BseBI | CCWGG | 1 cut(s) | 280 |
| BseDI | CCNNGG | 3 cut(s) | 105, 115, 174 |
| BseGI | GGATG | 1 cut(s) | 318 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 121, 299 |
| BseMI | GCAATG | 1 cut(s) | 305 |
| BseMII | CTCAG | 1 cut(s) | 84 |
| BseNI | ACTGG | 1 cut(s) | 352 |
| BsePI | GCGCGC | 1 cut(s) | 90 |
| BseRI | GAGGAG | 2 cut(s) | 382, 430 |
| Bsh1236I | CGCG | 4 cut(s) | 90, 92, 122, 150 |
| BshFI | GGCC | 3 cut(s) | 173, 179, 194 |
| BsiHKAI | GWGCWC | 1 cut(s) | 278 |
| BsiSI | CCGG | 3 cut(s) | 107, 117, 251 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 121, 299 |
| BsmAI | GTCTC | 1 cut(s) | 467 |
| BsnI | GGCC | 3 cut(s) | 173, 179, 194 |
| Bsp1286I | GDGCHC | 1 cut(s) | 278 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 8 cut(s) | 15, 48, 88, 122, 429, 444, 454, 483 |
| BspANI | GGCC | 3 cut(s) | 173, 179, 194 |
| BspCNI | CTCAG | 1 cut(s) | 83 |
| BspFNI | CGCG | 4 cut(s) | 90, 92, 122, 150 |
| BspQI | GCTCTTC | 1 cut(s) | 374 |
| BsrBI | CCGCTC | 1 cut(s) | 446 |
| BsrDI | GCAATG | 1 cut(s) | 305 |
| BsrI | ACTGG | 1 cut(s) | 352 |
| BssECI | CCNNGG | 3 cut(s) | 105, 115, 174 |
| BssHII | GCGCGC | 1 cut(s) | 90 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 1 cut(s) | 280 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 461 |
| Bst6I | CTCTTC | 1 cut(s) | 374 |
| BstC8I | GCNNGC | 6 cut(s) | 28, 32, 92, 263, 487, 491 |
| BstDEI | CTNAG | 1 cut(s) | 70 |
| BstF5I | GGATG | 1 cut(s) | 318 |
| BstFNI | CGCG | 4 cut(s) | 90, 92, 122, 150 |
| BstH2I | RGCGCY | 2 cut(s) | 474, 496 |
| BstHHI | GCGC | 6 cut(s) | 92, 94, 122, 150, 473, 495 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMAI | GTCTC | 1 cut(s) | 467 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 87, 428 |
| BstNI | CCWGG | 1 cut(s) | 280 |
| BstSCI | CCNGG | 4 cut(s) | 105, 115, 249, 278 |
| BstUI | CGCG | 4 cut(s) | 90, 92, 122, 150 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 363 |
| BsuRI | GGCC | 3 cut(s) | 173, 179, 194 |
| BtgZI | GCGATG | 1 cut(s) | 460 |
| BtsCI | GGATG | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 6 cut(s) | 28, 32, 92, 263, 487, 491 |
| CfoI | GCGC | 6 cut(s) | 92, 94, 122, 150, 473, 495 |
| Cfr13I | GGNCC | 2 cut(s) | 124, 178 |
| CseI | GACGC | 1 cut(s) | 32 |
| CviJI | RGCY | 7 cut(s) | 173, 179, 194, 220, 367, 386, 422 |
| CviKI_1 | RGCY | 7 cut(s) | 173, 179, 194, 220, 367, 386, 422 |
| DdeI | CTNAG | 1 cut(s) | 70 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| DriI | GACNNNNNGTC | 1 cut(s) | 296 |
| EaeI | YGGCCR | 1 cut(s) | 192 |
| Eam1104I | CTCTTC | 1 cut(s) | 374 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 296 |
| EarI | CTCTTC | 1 cut(s) | 374 |
| EciI | GGCGGA | 1 cut(s) | 37 |
| Eco47I | GGWCC | 1 cut(s) | 124 |
| Eco47III | AGCGCT | 1 cut(s) | 494 |
| EcoRII | CCWGG | 1 cut(s) | 278 |
| FaiI | YATR | 1 cut(s) | 53 |
| FauI | CCCGC | 2 cut(s) | 8, 461 |
| FokI | GGATG | 1 cut(s) | 305 |
| FspBI | CTAG | 3 cut(s) | 233, 294, 383 |
| GlaI | GCGC | 6 cut(s) | 91, 93, 121, 149, 472, 494 |
| GsuI | CTGGAG | 1 cut(s) | 451 |
| HaeII | RGCGCY | 2 cut(s) | 474, 496 |
| HaeIII | GGCC | 3 cut(s) | 173, 179, 194 |
| HapII | CCGG | 3 cut(s) | 107, 117, 251 |
| HgaI | GACGC | 1 cut(s) | 32 |
| HhaI | GCGC | 6 cut(s) | 92, 94, 122, 150, 473, 495 |
| Hin6I | GCGC | 6 cut(s) | 90, 92, 120, 148, 471, 493 |
| HinP1I | GCGC | 6 cut(s) | 90, 92, 120, 148, 471, 493 |
| HpaII | CCGG | 3 cut(s) | 107, 117, 251 |
| HphI | GGTGA | 1 cut(s) | 91 |
| Hpy166II | GTNNAC | 2 cut(s) | 99, 112 |
| Hpy8I | GTNNAC | 2 cut(s) | 99, 112 |
| HpyAV | CCTTC | 2 cut(s) | 137, 406 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 461 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 87, 428 |
| HpyF3I | CTNAG | 1 cut(s) | 70 |
| HspAI | GCGC | 6 cut(s) | 90, 92, 120, 148, 471, 493 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LguI | GCTCTTC | 1 cut(s) | 374 |
| LweI | GCATC | 1 cut(s) | 90 |
| MaeI | CTAG | 3 cut(s) | 233, 294, 383 |
| MaeIII | GTNAC | 2 cut(s) | 204, 267 |
| MalI | GATC | 1 cut(s) | 42 |
| MbiI | CCGCTC | 1 cut(s) | 446 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 3 cut(s) | 32, 50, 361 |
| MhlI | GDGCHC | 1 cut(s) | 278 |
| MlsI | TGGCCA | 1 cut(s) | 194 |
| MluNI | TGGCCA | 1 cut(s) | 194 |
| Mox20I | TGGCCA | 1 cut(s) | 194 |
| MscI | TGGCCA | 1 cut(s) | 194 |
| MslI | CAYNNNNRTG | 1 cut(s) | 200 |
| Msp20I | TGGCCA | 1 cut(s) | 194 |
| MspI | CCGG | 3 cut(s) | 107, 117, 251 |
| MspR9I | CCNGG | 4 cut(s) | 107, 117, 251, 280 |
| MvaI | CCWGG | 1 cut(s) | 280 |
| MvnI | CGCG | 4 cut(s) | 90, 92, 122, 150 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 87, 428 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 251 |
| NdeII | GATC | 1 cut(s) | 40 |
| NmeAIII | GCCGAG | 1 cut(s) | 199 |
| NmuCI | GTSAC | 2 cut(s) | 204, 267 |
| OliI | CACNNNNGTG | 1 cut(s) | 200 |
| PauI | GCGCGC | 1 cut(s) | 90 |
| PciSI | GCTCTTC | 1 cut(s) | 374 |
| Psp6I | CCWGG | 1 cut(s) | 278 |
| PspGI | CCWGG | 1 cut(s) | 278 |
| PspPI | GGNCC | 2 cut(s) | 124, 178 |
| PteI | GCGCGC | 1 cut(s) | 90 |
| RseI | CAYNNNNRTG | 1 cut(s) | 200 |
| SapI | GCTCTTC | 1 cut(s) | 374 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 2 cut(s) | 124, 178 |
| ScrFI | CCNGG | 4 cut(s) | 107, 117, 251, 280 |
| SduI | GDGCHC | 1 cut(s) | 278 |
| SetI | ASST | 3 cut(s) | 129, 222, 295 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| SinI | GGWCC | 1 cut(s) | 124 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 200 |
| SsiI | CCGC | 8 cut(s) | 15, 48, 88, 122, 429, 444, 454, 483 |
| SspMI | CTAG | 3 cut(s) | 233, 294, 383 |
| StyD4I | CCNGG | 4 cut(s) | 105, 115, 249, 278 |
| TaaI | ACNGT | 2 cut(s) | 11, 461 |
| TseFI | GTSAC | 2 cut(s) | 204, 267 |
| Tsp45I | GTSAC | 2 cut(s) | 204, 267 |
| TspDTI | ATGAA | 1 cut(s) | 307 |
| TspGWI | ACGGA | 1 cut(s) | 505 |
| VpaK11BI | GGWCC | 1 cut(s) | 124 |
| XspI | CTAG | 3 cut(s) | 233, 294, 383 |