No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 955 |
| Acc65I | GGTACC | 1 cut(s) | 926 |
| AccB1I | GGYRCC | 1 cut(s) | 926 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 423 |
| AccBSI | CCGCTC | 1 cut(s) | 1065 |
| AccII | CGCG | 4 cut(s) | 343, 394, 534, 886 |
| AclWI | GGATC | 2 cut(s) | 762, 1102 |
| AcoI | YGGCCR | 2 cut(s) | 142, 822 |
| AcyI | GRCGYC | 1 cut(s) | 548 |
| AfaI | GTAC | 5 cut(s) | 330, 340, 598, 928, 957 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 423, 638, 929 |
| AflIII | ACRYGT | 1 cut(s) | 335 |
| AgsI | TTSAA | 1 cut(s) | 1001 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 552 |
| AjnI | CCWGG | 5 cut(s) | 209, 372, 712, 908, 922 |
| AleI | CACNNNNGTG | 1 cut(s) | 830 |
| AluBI | AGCT | 6 cut(s) | 33, 69, 383, 742, 850, 988 |
| AluI | AGCT | 6 cut(s) | 33, 69, 383, 742, 850, 988 |
| Alw21I | GWGCWC | 1 cut(s) | 908 |
| Alw26I | GTCTC | 3 cut(s) | 542, 560, 680 |
| AlwI | GGATC | 2 cut(s) | 762, 1102 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 423 |
| Ama87I | CYCGRG | 1 cut(s) | 360 |
| AoxI | GGCC | 5 cut(s) | 142, 715, 801, 822, 1070 |
| ApeKI | GCWGC | 3 cut(s) | 699, 702, 705 |
| Asp718I | GGTACC | 1 cut(s) | 926 |
| AspLEI | GCGC | 3 cut(s) | 396, 886, 978 |
| AspS9I | GGNCC | 1 cut(s) | 967 |
| AsuC2I | CCSGG | 3 cut(s) | 21, 650, 678 |
| AsuHPI | GGTGA | 6 cut(s) | 5, 180, 277, 474, 652, 695 |
| AvaI | CYCGRG | 1 cut(s) | 360 |
| AvaII | GGWCC | 1 cut(s) | 967 |
| BaeGI | GKGCMC | 1 cut(s) | 1017 |
| BalI | TGGCCA | 2 cut(s) | 144, 824 |
| BanI | GGYRCC | 1 cut(s) | 926 |
| BanII | GRGCYC | 1 cut(s) | 361 |
| BauI | CACGAG | 2 cut(s) | 559, 830 |
| BbsI | GAAGAC | 1 cut(s) | 527 |
| Bbv12I | GWGCWC | 1 cut(s) | 908 |
| BbvI | GCAGC | 3 cut(s) | 711, 714, 717 |
| BccI | CCATC | 5 cut(s) | 100, 140, 175, 229, 259 |
| BciT130I | CCWGG | 5 cut(s) | 211, 374, 714, 910, 924 |
| BcnI | CCSGG | 3 cut(s) | 21, 650, 678 |
| BcoDI | GTCTC | 3 cut(s) | 542, 560, 680 |
| BfaI | CTAG | 4 cut(s) | 30, 983, 989, 1086 |
| BfuAI | ACCTGC | 1 cut(s) | 955 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 402, 714 |
| BisI | GCNGC | 4 cut(s) | 392, 700, 703, 706 |
| BlsI | GCNGC | 4 cut(s) | 393, 701, 704, 707 |
| Bme1390I | CCNGG | 8 cut(s) | 21, 211, 374, 650, 678, 714, 910, 924 |
| Bme18I | GGWCC | 1 cut(s) | 967 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 552 |
| BmeT110I | CYCGRG | 1 cut(s) | 360 |
| BmgT120I | GGNCC | 1 cut(s) | 967 |
| BmiI | GGNNCC | 2 cut(s) | 928, 969 |
| BmrFI | CCNGG | 8 cut(s) | 21, 211, 374, 650, 678, 714, 910, 924 |
| BmrI | ACTGGG | 1 cut(s) | 662 |
| BmsI | GCATC | 2 cut(s) | 414, 516 |
| BmuI | ACTGGG | 1 cut(s) | 662 |
| BpiI | GAAGAC | 1 cut(s) | 527 |
| BpmI | CTGGAG | 1 cut(s) | 884 |
| BpuEI | CTTGAG | 1 cut(s) | 1051 |
| BpuMI | CCSGG | 3 cut(s) | 21, 650, 678 |
| Bsa29I | ATCGAT | 1 cut(s) | 224 |
| BsaHI | GRCGYC | 1 cut(s) | 548 |
| BsaI | GGTCTC | 1 cut(s) | 542 |
| BsaJI | CCNNGG | 4 cut(s) | 209, 372, 676, 712 |
| BsaWI | WCCGGW | 1 cut(s) | 929 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 522, 552 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 423, 638, 929 |
| Bse1I | ACTGG | 3 cut(s) | 261, 668, 950 |
| Bse3DI | GCAATG | 1 cut(s) | 56 |
| BseBI | CCWGG | 5 cut(s) | 211, 374, 714, 910, 924 |
| BseCI | ATCGAT | 1 cut(s) | 224 |
| BseDI | CCNNGG | 4 cut(s) | 209, 372, 676, 712 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 423, 638, 929 |
| BseMI | GCAATG | 1 cut(s) | 56 |
| BseMII | CTCAG | 1 cut(s) | 605 |
| BseNI | ACTGG | 3 cut(s) | 261, 668, 950 |
| BseSI | GKGCMC | 1 cut(s) | 1017 |
| BseXI | GCAGC | 3 cut(s) | 711, 714, 717 |
| BseYI | CCCAGC | 3 cut(s) | 423, 631, 1017 |
| BsgI | GTGCAG | 1 cut(s) | 1037 |
| Bsh1236I | CGCG | 4 cut(s) | 343, 394, 534, 886 |
| BshFI | GGCC | 5 cut(s) | 144, 717, 803, 824, 1072 |
| BshNI | GGYRCC | 1 cut(s) | 926 |
| BshVI | ATCGAT | 1 cut(s) | 224 |
| BsiHKAI | GWGCWC | 1 cut(s) | 908 |
| BsiHKCI | CYCGRG | 1 cut(s) | 360 |
| BsiSI | CCGG | 4 cut(s) | 21, 649, 678, 930 |
| BslFI | GGGAC | 2 cut(s) | 429, 953 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 423, 638, 929 |
| BsmAI | GTCTC | 3 cut(s) | 542, 560, 680 |
| BsmFI | GGGAC | 2 cut(s) | 429, 953 |
| BsnI | GGCC | 5 cut(s) | 144, 717, 803, 824, 1072 |
| Bso31I | GGTCTC | 1 cut(s) | 542 |
| BsoBI | CYCGRG | 1 cut(s) | 360 |
| Bsp1286I | GDGCHC | 3 cut(s) | 361, 908, 1017 |
| Bsp143I | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| BspANI | GGCC | 5 cut(s) | 144, 717, 803, 824, 1072 |
| BspCNI | CTCAG | 1 cut(s) | 604 |
| BspDI | ATCGAT | 1 cut(s) | 224 |
| BspFNI | CGCG | 4 cut(s) | 343, 394, 534, 886 |
| BspLI | GGNNCC | 2 cut(s) | 928, 969 |
| BspMI | ACCTGC | 1 cut(s) | 955 |
| BspPI | GGATC | 2 cut(s) | 762, 1102 |
| BspT107I | GGYRCC | 1 cut(s) | 926 |
| BspTNI | GGTCTC | 1 cut(s) | 542 |
| BsrBI | CCGCTC | 1 cut(s) | 1065 |
| BsrDI | GCAATG | 1 cut(s) | 56 |
| BsrI | ACTGG | 3 cut(s) | 261, 668, 950 |
| BssECI | CCNNGG | 4 cut(s) | 209, 372, 676, 712 |
| BssMI | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| BssNI | GRCGYC | 1 cut(s) | 548 |
| BssSI | CACGAG | 2 cut(s) | 559, 830 |
| Bst2BI | CACGAG | 2 cut(s) | 559, 830 |
| Bst2UI | CCWGG | 5 cut(s) | 211, 374, 714, 910, 924 |
| Bst4CI | ACNGT | 1 cut(s) | 840 |
| BstACI | GRCGYC | 1 cut(s) | 548 |
| BstC8I | GCNNGC | 4 cut(s) | 345, 572, 744, 893 |
| BstDEI | CTNAG | 2 cut(s) | 502, 591 |
| BstFNI | CGCG | 4 cut(s) | 343, 394, 534, 886 |
| BstHHI | GCGC | 3 cut(s) | 396, 886, 978 |
| BstKTI | GATC | 4 cut(s) | 470, 604, 757, 1097 |
| BstMAI | GTCTC | 3 cut(s) | 542, 560, 680 |
| BstMBI | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 402, 705, 714, 892, 1012 |
| BstNI | CCWGG | 5 cut(s) | 211, 374, 714, 910, 924 |
| BstNSI | RCATGY | 3 cut(s) | 339, 391, 964 |
| BstSCI | CCNGG | 8 cut(s) | 19, 209, 372, 648, 676, 712, 908, 922 |
| BstSLI | GKGCMC | 1 cut(s) | 1017 |
| BstUI | CGCG | 4 cut(s) | 343, 394, 534, 886 |
| BstV1I | GCAGC | 3 cut(s) | 711, 714, 717 |
| BstV2I | GAAGAC | 1 cut(s) | 527 |
| BstX2I | RGATCY | 1 cut(s) | 754 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 210 |
| BstYI | RGATCY | 1 cut(s) | 754 |
| Bsu15I | ATCGAT | 1 cut(s) | 224 |
| BsuRI | GGCC | 5 cut(s) | 144, 717, 803, 824, 1072 |
| BsuTUI | ATCGAT | 1 cut(s) | 224 |
| BtgZI | GCGATG | 1 cut(s) | 78 |
| BtsI | GCAGTG | 2 cut(s) | 971, 1049 |
| BtsIMutI | CAGTG | 3 cut(s) | 268, 971, 1049 |
| BveI | ACCTGC | 1 cut(s) | 955 |
| Cac8I | GCNNGC | 4 cut(s) | 345, 572, 744, 893 |
| CaiI | CAGNNNCTG | 1 cut(s) | 423 |
| CfoI | GCGC | 3 cut(s) | 396, 886, 978 |
| Cfr13I | GGNCC | 1 cut(s) | 967 |
| ClaI | ATCGAT | 1 cut(s) | 224 |
| CseI | GACGC | 2 cut(s) | 429, 556 |
| CsiI | ACCWGGT | 1 cut(s) | 922 |
| Csp6I | GTAC | 5 cut(s) | 329, 339, 597, 927, 956 |
| CviAII | CATG | 5 cut(s) | 295, 336, 388, 961, 1034 |
| CviQI | GTAC | 5 cut(s) | 329, 339, 597, 927, 956 |
| DdeI | CTNAG | 2 cut(s) | 502, 591 |
| DpnI | GATC | 4 cut(s) | 469, 603, 756, 1096 |
| DpnII | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| DriI | GACNNNNNGTC | 1 cut(s) | 552 |
| EaeI | YGGCCR | 2 cut(s) | 142, 822 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 552 |
| EciI | GGCGGA | 1 cut(s) | 767 |
| Eco147I | AGGCCT | 1 cut(s) | 1072 |
| Eco24I | GRGCYC | 1 cut(s) | 361 |
| Eco31I | GGTCTC | 1 cut(s) | 542 |
| Eco47I | GGWCC | 1 cut(s) | 967 |
| Eco88I | CYCGRG | 1 cut(s) | 360 |
| EcoO109I | RGGNCCY | 1 cut(s) | 967 |
| EcoRII | CCWGG | 5 cut(s) | 209, 372, 712, 908, 922 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| EcoT38I | GRGCYC | 1 cut(s) | 361 |
| FaeI | CATG | 5 cut(s) | 298, 339, 391, 964, 1037 |
| FaqI | GGGAC | 2 cut(s) | 429, 953 |
| FatI | CATG | 5 cut(s) | 294, 335, 387, 960, 1033 |
| FauI | CCCGC | 2 cut(s) | 336, 1083 |
| Fnu4HI | GCNGC | 4 cut(s) | 392, 700, 703, 706 |
| FriOI | GRGCYC | 1 cut(s) | 361 |
| Fsp4HI | GCNGC | 4 cut(s) | 392, 700, 703, 706 |
| FspBI | CTAG | 4 cut(s) | 30, 983, 989, 1086 |
| GlaI | GCGC | 3 cut(s) | 395, 885, 977 |
| GluI | GCNGC | 4 cut(s) | 392, 700, 703, 706 |
| GsaI | CCCAGC | 3 cut(s) | 427, 635, 1021 |
| GsuI | CTGGAG | 1 cut(s) | 884 |
| HaeIII | GGCC | 5 cut(s) | 144, 717, 803, 824, 1072 |
| HapII | CCGG | 4 cut(s) | 21, 649, 678, 930 |
| HgaI | GACGC | 2 cut(s) | 429, 556 |
| HhaI | GCGC | 3 cut(s) | 396, 886, 978 |
| Hin1I | GRCGYC | 1 cut(s) | 548 |
| Hin1II | CATG | 5 cut(s) | 298, 339, 391, 964, 1037 |
| Hin6I | GCGC | 3 cut(s) | 394, 884, 976 |
| HinP1I | GCGC | 3 cut(s) | 394, 884, 976 |
| HincII | GTYRAC | 1 cut(s) | 673 |
| HindII | GTYRAC | 1 cut(s) | 673 |
| HinfI | GANTC | 1 cut(s) | 1043 |
| HpaII | CCGG | 4 cut(s) | 21, 649, 678, 930 |
| HphI | GGTGA | 6 cut(s) | 5, 180, 277, 474, 652, 695 |
| Hpy166II | GTNNAC | 5 cut(s) | 609, 660, 673, 782, 958 |
| Hpy188I | TCNGA | 4 cut(s) | 410, 512, 546, 594 |
| Hpy188III | TCNNGA | 1 cut(s) | 417 |
| Hpy8I | GTNNAC | 5 cut(s) | 609, 660, 673, 782, 958 |
| HpyAV | CCTTC | 1 cut(s) | 54 |
| HpyCH4III | ACNGT | 1 cut(s) | 840 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 61, 964, 1054 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 402, 705, 714, 892, 1012 |
| HpyF3I | CTNAG | 2 cut(s) | 502, 591 |
| Hsp92I | GRCGYC | 1 cut(s) | 548 |
| Hsp92II | CATG | 5 cut(s) | 298, 339, 391, 964, 1037 |
| HspAI | GCGC | 3 cut(s) | 394, 884, 976 |
| KpnI | GGTACC | 1 cut(s) | 930 |
| Kzo9I | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| LmnI | GCTCC | 3 cut(s) | 328, 903, 932 |
| Lsp1109I | GCAGC | 3 cut(s) | 711, 714, 717 |
| LweI | GCATC | 2 cut(s) | 414, 516 |
| MabI | ACCWGGT | 1 cut(s) | 922 |
| MaeI | CTAG | 4 cut(s) | 30, 983, 989, 1086 |
| MaeIII | GTNAC | 6 cut(s) | 104, 376, 493, 563, 834, 897 |
| MalI | GATC | 4 cut(s) | 469, 603, 756, 1096 |
| MbiI | CCGCTC | 1 cut(s) | 1065 |
| MboI | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| MboII | GAAGA | 5 cut(s) | 274, 474, 477, 532, 749 |
| MflI | RGATCY | 1 cut(s) | 754 |
| MhlI | GDGCHC | 3 cut(s) | 361, 908, 1017 |
| MlsI | TGGCCA | 2 cut(s) | 144, 824 |
| MluCI | AATT | 5 cut(s) | 50, 86, 161, 255, 996 |
| MluNI | TGGCCA | 2 cut(s) | 144, 824 |
| MlyI | GAGTC | 1 cut(s) | 1052 |
| MmeI | TCCRAC | 1 cut(s) | 1008 |
| Mox20I | TGGCCA | 2 cut(s) | 144, 824 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MscI | TGGCCA | 2 cut(s) | 144, 824 |
| MseI | TTAA | 1 cut(s) | 645 |
| MslI | CAYNNNNRTG | 2 cut(s) | 243, 830 |
| Msp20I | TGGCCA | 2 cut(s) | 144, 824 |
| MspA1I | CMGCKG | 1 cut(s) | 635 |
| MspI | CCGG | 4 cut(s) | 21, 649, 678, 930 |
| MspR9I | CCNGG | 8 cut(s) | 21, 211, 374, 650, 678, 714, 910, 924 |
| MvaI | CCWGG | 5 cut(s) | 211, 374, 714, 910, 924 |
| MvnI | CGCG | 4 cut(s) | 343, 394, 534, 886 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 402, 705, 714, 892, 1012 |
| NciI | CCSGG | 3 cut(s) | 21, 650, 678 |
| NdeII | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| NlaIII | CATG | 5 cut(s) | 298, 339, 391, 964, 1037 |
| NlaIV | GGNNCC | 2 cut(s) | 928, 969 |
| NmuCI | GTSAC | 4 cut(s) | 376, 493, 834, 897 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 3 cut(s) | 339, 391, 964 |
| OliI | CACNNNNGTG | 1 cut(s) | 830 |
| PceI | AGGCCT | 1 cut(s) | 1072 |
| PciI | ACATGT | 1 cut(s) | 335 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 423 |
| PkrI | GCNGC | 4 cut(s) | 393, 701, 704, 707 |
| PleI | GAGTC | 1 cut(s) | 1051 |
| PpsI | GAGTC | 1 cut(s) | 1051 |
| PpuMI | RGGWCCY | 1 cut(s) | 967 |
| PscI | ACATGT | 1 cut(s) | 335 |
| Psp5II | RGGWCCY | 1 cut(s) | 967 |
| Psp6I | CCWGG | 5 cut(s) | 209, 372, 712, 908, 922 |
| PspFI | CCCAGC | 3 cut(s) | 423, 631, 1017 |
| PspGI | CCWGG | 5 cut(s) | 209, 372, 712, 908, 922 |
| PspN4I | GGNNCC | 2 cut(s) | 928, 969 |
| PspPI | GGNCC | 1 cut(s) | 967 |
| PspPPI | RGGWCCY | 1 cut(s) | 967 |
| PstNI | CAGNNNCTG | 1 cut(s) | 423 |
| PsuI | RGATCY | 1 cut(s) | 754 |
| RsaI | GTAC | 5 cut(s) | 330, 340, 598, 928, 957 |
| RsaNI | GTAC | 5 cut(s) | 329, 339, 597, 927, 956 |
| RseI | CAYNNNNRTG | 2 cut(s) | 243, 830 |
| SaqAI | TTAA | 1 cut(s) | 645 |
| SatI | GCNGC | 4 cut(s) | 392, 700, 703, 706 |
| Sau3AI | GATC | 4 cut(s) | 467, 601, 754, 1094 |
| Sau96I | GGNCC | 1 cut(s) | 967 |
| SchI | GAGTC | 1 cut(s) | 1052 |
| ScrFI | CCNGG | 8 cut(s) | 21, 211, 374, 650, 678, 714, 910, 924 |
| SduI | GDGCHC | 3 cut(s) | 361, 908, 1017 |
| SexAI | ACCWGGT | 1 cut(s) | 922 |
| SfaNI | GCATC | 2 cut(s) | 414, 516 |
| SinI | GGWCC | 1 cut(s) | 967 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 243, 830 |
| SmlI | CTYRAG | 1 cut(s) | 1066 |
| SmoI | CTYRAG | 1 cut(s) | 1066 |
| Sse9I | AATT | 5 cut(s) | 50, 86, 161, 255, 996 |
| SseBI | AGGCCT | 1 cut(s) | 1072 |
| SspMI | CTAG | 4 cut(s) | 30, 983, 989, 1086 |
| StuI | AGGCCT | 1 cut(s) | 1072 |
| StyD4I | CCNGG | 8 cut(s) | 19, 209, 372, 648, 676, 712, 908, 922 |
| TaaI | ACNGT | 1 cut(s) | 840 |
| TaqI | TCGA | 3 cut(s) | 224, 431, 452 |
| TasI | AATT | 5 cut(s) | 50, 86, 161, 255, 996 |
| TauI | GCSGC | 1 cut(s) | 394 |
| Tru1I | TTAA | 1 cut(s) | 645 |
| Tru9I | TTAA | 1 cut(s) | 645 |
| TscAI | CASTG | 3 cut(s) | 268, 978, 1056 |
| TseFI | GTSAC | 4 cut(s) | 376, 493, 834, 897 |
| TseI | GCWGC | 3 cut(s) | 699, 702, 705 |
| Tsp45I | GTSAC | 4 cut(s) | 376, 493, 834, 897 |
| TspDTI | ATGAA | 2 cut(s) | 257, 268 |
| TspGWI | ACGGA | 2 cut(s) | 264, 916 |
| TspRI | CASTG | 3 cut(s) | 268, 978, 1056 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 423 |
| VpaK11BI | GGWCC | 1 cut(s) | 967 |
| XceI | RCATGY | 3 cut(s) | 339, 391, 964 |
| XspI | CTAG | 4 cut(s) | 30, 983, 989, 1086 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |