Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 820 |
| Acc36I | ACCTGC | 2 cut(s) | 820, 967 |
| AccB1I | GGYRCC | 1 cut(s) | 1241 |
| AccBSI | CCGCTC | 2 cut(s) | 513, 1116 |
| AccIII | TCCGGA | 1 cut(s) | 303 |
| AclWI | GGATC | 3 cut(s) | 49, 89, 1136 |
| AcoI | YGGCCR | 2 cut(s) | 834, 1149 |
| AcsI | RAATTY | 1 cut(s) | 481 |
| AcuI | CTGAAG | 1 cut(s) | 102 |
| AcyI | GRCGYC | 1 cut(s) | 1242 |
| AfaI | GTAC | 4 cut(s) | 12, 940, 969, 1320 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 775, 829, 941, 1120, 1125 |
| AgsI | TTSAA | 5 cut(s) | 247, 346, 481, 670, 1252 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 107 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 254, 286 |
| AluBI | AGCT | 5 cut(s) | 107, 181, 754, 1000, 1176 |
| AluI | AGCT | 5 cut(s) | 107, 181, 754, 1000, 1176 |
| Alw21I | GWGCWC | 1 cut(s) | 920 |
| Alw26I | GTCTC | 4 cut(s) | 527, 556, 692, 1076 |
| AlwI | GGATC | 3 cut(s) | 49, 89, 1136 |
| Ama87I | CYCGRG | 1 cut(s) | 1079 |
| Aor13HI | TCCGGA | 1 cut(s) | 303 |
| AoxI | GGCC | 9 cut(s) | 587, 727, 813, 819, 834, 1013, 1149, 1154, 1272 |
| ApeKI | GCWGC | 6 cut(s) | 445, 554, 714, 1064, 1067, 1196 |
| ApoI | RAATTY | 1 cut(s) | 481 |
| AspLEI | GCGC | 6 cut(s) | 398, 445, 554, 804, 947, 1244 |
| AspS9I | GGNCC | 7 cut(s) | 766, 820, 979, 988, 1013, 1155, 1278 |
| AsuC2I | CCSGG | 2 cut(s) | 1148, 1239 |
| AsuHPI | GGTGA | 4 cut(s) | 637, 667, 707, 730 |
| AvaI | CYCGRG | 1 cut(s) | 1079 |
| AvaII | GGWCC | 4 cut(s) | 766, 979, 988, 1278 |
| BaeGI | GKGCMC | 2 cut(s) | 1030, 1148 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 828, 861 |
| BalI | TGGCCA | 1 cut(s) | 836 |
| BanI | GGYRCC | 1 cut(s) | 1241 |
| BanII | GRGCYC | 1 cut(s) | 626 |
| BbsI | GAAGAC | 1 cut(s) | 526 |
| Bbv12I | GWGCWC | 1 cut(s) | 920 |
| BbvI | GCAGC | 6 cut(s) | 432, 541, 726, 1051, 1079, 1208 |
| BccI | CCATC | 1 cut(s) | 1003 |
| BceAI | ACGGC | 2 cut(s) | 368, 453 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1042, 1076 |
| BcnI | CCSGG | 2 cut(s) | 1148, 1239 |
| BcoDI | GTCTC | 4 cut(s) | 527, 556, 692, 1076 |
| BfaI | CTAG | 2 cut(s) | 164, 936 |
| BfmI | CTRYAG | 2 cut(s) | 1065, 1299 |
| BfoI | RGCGCY | 3 cut(s) | 446, 555, 1245 |
| BfuAI | ACCTGC | 2 cut(s) | 820, 967 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 726, 893, 1162 |
| Bme18I | GGWCC | 4 cut(s) | 766, 979, 988, 1278 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 107 |
| BmeT110I | CYCGRG | 1 cut(s) | 1079 |
| BmgT120I | GGNCC | 7 cut(s) | 766, 820, 979, 988, 1013, 1155, 1278 |
| BmiI | GGNNCC | 6 cut(s) | 623, 981, 990, 1014, 1157, 1243 |
| BmrI | ACTGGG | 2 cut(s) | 332, 1025 |
| BmsI | GCATC | 3 cut(s) | 503, 1157, 1183 |
| BmuI | ACTGGG | 2 cut(s) | 332, 1025 |
| BpiI | GAAGAC | 1 cut(s) | 526 |
| BpmI | CTGGAG | 1 cut(s) | 896 |
| BpuMI | CCSGG | 2 cut(s) | 1148, 1239 |
| BsaBI | GATNNNNATC | 1 cut(s) | 59 |
| BsaHI | GRCGYC | 1 cut(s) | 1242 |
| BsaI | GGTCTC | 2 cut(s) | 556, 1076 |
| BsaJI | CCNNGG | 8 cut(s) | 593, 594, 649, 688, 816, 1099, 1119, 1237 |
| BsaWI | WCCGGW | 1 cut(s) | 303 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 524, 554, 1197, 1227 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 775, 829, 941, 1120, 1125 |
| Bse118I | RCCGGY | 2 cut(s) | 4, 941 |
| Bse1I | ACTGG | 8 cut(s) | 338, 367, 765, 962, 1031, 1166, 1189, 1318 |
| Bse3DI | GCAATG | 1 cut(s) | 135 |
| Bse8I | GATNNNNATC | 1 cut(s) | 59 |
| BseAI | TCCGGA | 1 cut(s) | 303 |
| BseDI | CCNNGG | 8 cut(s) | 593, 594, 649, 688, 816, 1099, 1119, 1237 |
| BseGI | GGATG | 3 cut(s) | 20, 1014, 1198 |
| BseJI | GATNNNNATC | 1 cut(s) | 59 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 775, 829, 941, 1120, 1125 |
| BseMI | GCAATG | 1 cut(s) | 135 |
| BseMII | CTCAG | 2 cut(s) | 201, 489 |
| BseNI | ACTGG | 8 cut(s) | 338, 367, 765, 962, 1031, 1166, 1189, 1318 |
| BseRI | GAGGAG | 2 cut(s) | 378, 464 |
| BseSI | GKGCMC | 2 cut(s) | 1030, 1148 |
| BseXI | GCAGC | 6 cut(s) | 432, 541, 726, 1051, 1079, 1208 |
| BseYI | CCCAGC | 1 cut(s) | 1220 |
| BshFI | GGCC | 9 cut(s) | 589, 729, 815, 821, 836, 1015, 1151, 1156, 1274 |
| BshNI | GGYRCC | 1 cut(s) | 1241 |
| BsiHKAI | GWGCWC | 1 cut(s) | 920 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1079 |
| BsiSI | CCGG | 5 cut(s) | 5, 304, 942, 1148, 1239 |
| BsiWI | CGTACG | 1 cut(s) | 1318 |
| BslFI | GGGAC | 1 cut(s) | 965 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 775, 829, 941, 1120, 1125 |
| BsmAI | GTCTC | 4 cut(s) | 527, 556, 692, 1076 |
| BsmFI | GGGAC | 1 cut(s) | 965 |
| BsnI | GGCC | 9 cut(s) | 589, 729, 815, 821, 836, 1015, 1151, 1156, 1274 |
| Bso31I | GGTCTC | 2 cut(s) | 556, 1076 |
| BsoBI | CYCGRG | 1 cut(s) | 1079 |
| Bsp1286I | GDGCHC | 4 cut(s) | 626, 920, 1030, 1148 |
| Bsp13I | TCCGGA | 1 cut(s) | 303 |
| Bsp1407I | TGTACA | 1 cut(s) | 967 |
| Bsp143I | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| BspANI | GGCC | 9 cut(s) | 589, 729, 815, 821, 836, 1015, 1151, 1156, 1274 |
| BspCNI | CTCAG | 2 cut(s) | 202, 490 |
| BspEI | TCCGGA | 1 cut(s) | 303 |
| BspLI | GGNNCC | 6 cut(s) | 623, 981, 990, 1014, 1157, 1243 |
| BspMAI | CTGCAG | 1 cut(s) | 1069 |
| BspMI | ACCTGC | 2 cut(s) | 820, 967 |
| BspPI | GGATC | 3 cut(s) | 49, 89, 1136 |
| BspQI | GCTCTTC | 1 cut(s) | 405 |
| BspT107I | GGYRCC | 1 cut(s) | 1241 |
| BspTNI | GGTCTC | 2 cut(s) | 556, 1076 |
| BsrBI | CCGCTC | 2 cut(s) | 513, 1116 |
| BsrDI | GCAATG | 1 cut(s) | 135 |
| BsrFI | RCCGGY | 2 cut(s) | 4, 941 |
| BsrGI | TGTACA | 1 cut(s) | 967 |
| BsrI | ACTGG | 8 cut(s) | 338, 367, 765, 962, 1031, 1166, 1189, 1318 |
| BssAI | RCCGGY | 2 cut(s) | 4, 941 |
| BssECI | CCNNGG | 8 cut(s) | 593, 594, 649, 688, 816, 1099, 1119, 1237 |
| BssMI | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| BssNI | GRCGYC | 1 cut(s) | 1242 |
| BssT1I | CCWWGG | 1 cut(s) | 649 |
| Bst4CI | ACNGT | 2 cut(s) | 220, 909 |
| Bst6I | CTCTTC | 1 cut(s) | 405 |
| BstACI | GRCGYC | 1 cut(s) | 1242 |
| BstAUI | TGTACA | 1 cut(s) | 967 |
| BstC8I | GCNNGC | 4 cut(s) | 6, 258, 659, 756 |
| BstDEI | CTNAG | 3 cut(s) | 210, 498, 863 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 773 |
| BstF5I | GGATG | 3 cut(s) | 20, 1014, 1198 |
| BstH2I | RGCGCY | 3 cut(s) | 446, 555, 1245 |
| BstHHI | GCGC | 6 cut(s) | 398, 445, 554, 804, 947, 1244 |
| BstKTI | GATC | 4 cut(s) | 57, 84, 378, 1131 |
| BstMAI | GTCTC | 4 cut(s) | 527, 556, 692, 1076 |
| BstMBI | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 104, 113, 462, 621, 726, 893, 982, 1162, 1205 |
| BstNSI | RCATGY | 1 cut(s) | 260 |
| BstSFI | CTRYAG | 2 cut(s) | 1065, 1299 |
| BstSLI | GKGCMC | 2 cut(s) | 1030, 1148 |
| BstV1I | GCAGC | 6 cut(s) | 432, 541, 726, 1051, 1079, 1208 |
| BstV2I | GAAGAC | 1 cut(s) | 526 |
| BsuRI | GGCC | 9 cut(s) | 589, 729, 815, 821, 836, 1015, 1151, 1156, 1274 |
| BtgZI | GCGATG | 3 cut(s) | 614, 1045, 1180 |
| BtsCI | GGATG | 3 cut(s) | 20, 1014, 1198 |
| BtsIMutI | CAGTG | 4 cut(s) | 91, 360, 758, 1159 |
| BveI | ACCTGC | 2 cut(s) | 820, 967 |
| Cac8I | GCNNGC | 4 cut(s) | 6, 258, 659, 756 |
| CfoI | GCGC | 6 cut(s) | 398, 445, 554, 804, 947, 1244 |
| Cfr10I | RCCGGY | 2 cut(s) | 4, 941 |
| Cfr13I | GGNCC | 7 cut(s) | 766, 820, 979, 988, 1013, 1155, 1278 |
| CseI | GACGC | 2 cut(s) | 531, 546 |
| Csp6I | GTAC | 4 cut(s) | 11, 939, 968, 1319 |
| CviAII | CATG | 4 cut(s) | 112, 232, 257, 393 |
| CviQI | GTAC | 4 cut(s) | 11, 939, 968, 1319 |
| DdeI | CTNAG | 3 cut(s) | 210, 498, 863 |
| DinI | GGCGCC | 1 cut(s) | 1243 |
| DpnI | GATC | 4 cut(s) | 56, 83, 377, 1130 |
| DpnII | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| DriI | GACNNNNNGTC | 1 cut(s) | 107 |
| EaeI | YGGCCR | 2 cut(s) | 834, 1149 |
| Eam1104I | CTCTTC | 1 cut(s) | 405 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 107 |
| EarI | CTCTTC | 1 cut(s) | 405 |
| EciI | GGCGGA | 1 cut(s) | 779 |
| Eco130I | CCWWGG | 1 cut(s) | 649 |
| Eco24I | GRGCYC | 1 cut(s) | 626 |
| Eco31I | GGTCTC | 2 cut(s) | 556, 1076 |
| Eco47I | GGWCC | 4 cut(s) | 766, 979, 988, 1278 |
| Eco57I | CTGAAG | 1 cut(s) | 102 |
| Eco88I | CYCGRG | 1 cut(s) | 1079 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 773 |
| EcoO109I | RGGNCCY | 2 cut(s) | 979, 1155 |
| EcoRI | GAATTC | 1 cut(s) | 481 |
| EcoT14I | CCWWGG | 1 cut(s) | 649 |
| EcoT22I | ATGCAT | 1 cut(s) | 258 |
| EcoT38I | GRGCYC | 1 cut(s) | 626 |
| EgeI | GGCGCC | 1 cut(s) | 1243 |
| EheI | GGCGCC | 1 cut(s) | 1243 |
| ErhI | CCWWGG | 1 cut(s) | 649 |
| FaeI | CATG | 4 cut(s) | 115, 235, 260, 396 |
| FaqI | GGGAC | 1 cut(s) | 965 |
| FatI | CATG | 4 cut(s) | 111, 231, 256, 392 |
| FauI | CCCGC | 2 cut(s) | 990, 1121 |
| FauNDI | CATATG | 1 cut(s) | 972 |
| FokI | GGATG | 3 cut(s) | 7, 1021, 1205 |
| FriOI | GRGCYC | 1 cut(s) | 626 |
| FspBI | CTAG | 2 cut(s) | 164, 936 |
| GlaI | GCGC | 6 cut(s) | 397, 444, 553, 803, 946, 1243 |
| GsaI | CCCAGC | 1 cut(s) | 1224 |
| GsuI | CTGGAG | 1 cut(s) | 896 |
| HaeII | RGCGCY | 3 cut(s) | 446, 555, 1245 |
| HaeIII | GGCC | 9 cut(s) | 589, 729, 815, 821, 836, 1015, 1151, 1156, 1274 |
| HapII | CCGG | 5 cut(s) | 5, 304, 942, 1148, 1239 |
| HgaI | GACGC | 2 cut(s) | 531, 546 |
| HhaI | GCGC | 6 cut(s) | 398, 445, 554, 804, 947, 1244 |
| Hin1I | GRCGYC | 1 cut(s) | 1242 |
| Hin1II | CATG | 4 cut(s) | 115, 235, 260, 396 |
| Hin6I | GCGC | 6 cut(s) | 396, 443, 552, 802, 945, 1242 |
| HinP1I | GCGC | 6 cut(s) | 396, 443, 552, 802, 945, 1242 |
| HinfI | GANTC | 6 cut(s) | 144, 426, 582, 1056, 1227, 1328 |
| HpaII | CCGG | 5 cut(s) | 5, 304, 942, 1148, 1239 |
| HphI | GGTGA | 4 cut(s) | 637, 667, 707, 730 |
| Hpy166II | GTNNAC | 3 cut(s) | 89, 685, 794 |
| Hpy188I | TCNGA | 6 cut(s) | 121, 143, 322, 499, 581, 1311 |
| Hpy188III | TCNNGA | 5 cut(s) | 304, 478, 561, 1079, 1081 |
| Hpy8I | GTNNAC | 3 cut(s) | 89, 685, 794 |
| Hpy99I | CGWCG | 3 cut(s) | 354, 426, 562 |
| HpyAV | CCTTC | 1 cut(s) | 779 |
| HpyCH4III | ACNGT | 2 cut(s) | 220, 909 |
| HpyCH4IV | ACGT | 3 cut(s) | 790, 839, 850 |
| HpyCH4V | TGCA | 9 cut(s) | 128, 256, 448, 661, 976, 1067, 1170, 1196, 1293 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 104, 113, 462, 621, 726, 893, 982, 1162, 1205 |
| HpyF3I | CTNAG | 3 cut(s) | 210, 498, 863 |
| HpySE526I | ACGT | 3 cut(s) | 790, 839, 850 |
| Hsp92I | GRCGYC | 1 cut(s) | 1242 |
| Hsp92II | CATG | 4 cut(s) | 115, 235, 260, 396 |
| HspAI | GCGC | 6 cut(s) | 396, 443, 552, 802, 945, 1242 |
| KasI | GGCGCC | 1 cut(s) | 1241 |
| Kpn2I | TCCGGA | 1 cut(s) | 303 |
| KroI | GCCGGC | 1 cut(s) | 4 |
| KroNI | GCCGGC | 1 cut(s) | 6 |
| Kzo9I | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| LguI | GCTCTTC | 1 cut(s) | 405 |
| LmnI | GCTCC | 6 cut(s) | 133, 621, 900, 915, 1121, 1205 |
| Lsp1109I | GCAGC | 6 cut(s) | 432, 541, 726, 1051, 1079, 1208 |
| LweI | GCATC | 3 cut(s) | 503, 1157, 1183 |
| MaeI | CTAG | 2 cut(s) | 164, 936 |
| MaeII | ACGT | 3 cut(s) | 790, 839, 850 |
| MaeIII | GTNAC | 3 cut(s) | 673, 909, 1339 |
| MalI | GATC | 4 cut(s) | 56, 83, 377, 1130 |
| MbiI | CCGCTC | 2 cut(s) | 513, 1116 |
| MboI | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| MboII | GAAGA | 4 cut(s) | 274, 392, 531, 879 |
| MhlI | GDGCHC | 4 cut(s) | 626, 920, 1030, 1148 |
| MlsI | TGGCCA | 1 cut(s) | 836 |
| MluCI | AATT | 4 cut(s) | 150, 481, 637, 1246 |
| MluNI | TGGCCA | 1 cut(s) | 836 |
| Mly113I | GGCGCC | 1 cut(s) | 1242 |
| MlyI | GAGTC | 2 cut(s) | 1236, 1322 |
| MmeI | TCCRAC | 1 cut(s) | 1183 |
| Mox20I | TGGCCA | 1 cut(s) | 836 |
| Mph1103I | ATGCAT | 1 cut(s) | 258 |
| MroI | TCCGGA | 1 cut(s) | 303 |
| MroNI | GCCGGC | 1 cut(s) | 4 |
| MscI | TGGCCA | 1 cut(s) | 836 |
| MseI | TTAA | 3 cut(s) | 149, 1035, 1215 |
| Msp20I | TGGCCA | 1 cut(s) | 836 |
| MspA1I | CMGCKG | 4 cut(s) | 985, 1139, 1224, 1351 |
| MspI | CCGG | 5 cut(s) | 5, 304, 942, 1148, 1239 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 104, 113, 462, 621, 726, 893, 982, 1162, 1205 |
| NaeI | GCCGGC | 1 cut(s) | 6 |
| NarI | GGCGCC | 1 cut(s) | 1242 |
| NciI | CCSGG | 2 cut(s) | 1148, 1239 |
| NdeI | CATATG | 1 cut(s) | 972 |
| NdeII | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| NgoMIV | GCCGGC | 1 cut(s) | 4 |
| NlaIII | CATG | 4 cut(s) | 115, 235, 260, 396 |
| NlaIV | GGNNCC | 6 cut(s) | 623, 981, 990, 1014, 1157, 1243 |
| NmeAIII | GCCGAG | 1 cut(s) | 841 |
| NmuCI | GTSAC | 3 cut(s) | 673, 909, 1339 |
| NsiI | ATGCAT | 1 cut(s) | 258 |
| NspI | RCATGY | 1 cut(s) | 260 |
| PaeI | GCATGC | 1 cut(s) | 260 |
| PaeR7I | CTCGAG | 1 cut(s) | 1079 |
| PaqCI | CACCTGC | 1 cut(s) | 820 |
| PasI | CCCWGGG | 1 cut(s) | 594 |
| PciSI | GCTCTTC | 1 cut(s) | 405 |
| PdiI | GCCGGC | 1 cut(s) | 6 |
| PfeI | GAWTC | 4 cut(s) | 144, 426, 582, 1056 |
| Pfl23II | CGTACG | 1 cut(s) | 1318 |
| PleI | GAGTC | 2 cut(s) | 1235, 1322 |
| PluTI | GGCGCC | 1 cut(s) | 1245 |
| PpsI | GAGTC | 2 cut(s) | 1235, 1322 |
| PpuMI | RGGWCCY | 1 cut(s) | 979 |
| Psp5II | RGGWCCY | 1 cut(s) | 979 |
| PspFI | CCCAGC | 1 cut(s) | 1220 |
| PspLI | CGTACG | 1 cut(s) | 1318 |
| PspN4I | GGNNCC | 6 cut(s) | 623, 981, 990, 1014, 1157, 1243 |
| PspPI | GGNCC | 7 cut(s) | 766, 820, 979, 988, 1013, 1155, 1278 |
| PspPPI | RGGWCCY | 1 cut(s) | 979 |
| PstI | CTGCAG | 1 cut(s) | 1069 |
| RsaI | GTAC | 4 cut(s) | 12, 940, 969, 1320 |
| RsaNI | GTAC | 4 cut(s) | 11, 939, 968, 1319 |
| SapI | GCTCTTC | 1 cut(s) | 405 |
| SaqAI | TTAA | 3 cut(s) | 149, 1035, 1215 |
| Sau3AI | GATC | 4 cut(s) | 54, 81, 375, 1128 |
| Sau96I | GGNCC | 7 cut(s) | 766, 820, 979, 988, 1013, 1155, 1278 |
| SchI | GAGTC | 2 cut(s) | 1236, 1322 |
| SduI | GDGCHC | 4 cut(s) | 626, 920, 1030, 1148 |
| SfaNI | GCATC | 3 cut(s) | 503, 1157, 1183 |
| SfcI | CTRYAG | 2 cut(s) | 1065, 1299 |
| SfoI | GGCGCC | 1 cut(s) | 1243 |
| Sfr274I | CTCGAG | 1 cut(s) | 1079 |
| SinI | GGWCC | 4 cut(s) | 766, 979, 988, 1278 |
| SlaI | CTCGAG | 1 cut(s) | 1079 |
| SmlI | CTYRAG | 1 cut(s) | 1079 |
| SmoI | CTYRAG | 1 cut(s) | 1079 |
| SphI | GCATGC | 1 cut(s) | 260 |
| Sse9I | AATT | 4 cut(s) | 150, 481, 637, 1246 |
| SspDI | GGCGCC | 1 cut(s) | 1241 |
| SspMI | CTAG | 2 cut(s) | 164, 936 |
| StyI | CCWWGG | 1 cut(s) | 649 |
| TaaI | ACNGT | 2 cut(s) | 220, 909 |
| TaiI | ACGT | 3 cut(s) | 793, 842, 853 |
| TaqI | TCGA | 4 cut(s) | 100, 424, 560, 1080 |
| TasI | AATT | 4 cut(s) | 150, 481, 637, 1246 |
| TatI | WGTACW | 1 cut(s) | 967 |
| TauI | GCSGC | 6 cut(s) | 443, 456, 549, 552, 720, 1018 |
| TfiI | GAWTC | 4 cut(s) | 144, 426, 582, 1056 |
| Tru1I | TTAA | 3 cut(s) | 149, 1035, 1215 |
| Tru9I | TTAA | 3 cut(s) | 149, 1035, 1215 |
| TscAI | CASTG | 4 cut(s) | 91, 367, 765, 1166 |
| TseFI | GTSAC | 3 cut(s) | 673, 909, 1339 |
| TseI | GCWGC | 6 cut(s) | 445, 554, 714, 1064, 1067, 1196 |
| Tsp45I | GTSAC | 3 cut(s) | 673, 909, 1339 |
| TspDTI | ATGAA | 2 cut(s) | 761, 873 |
| TspGWI | ACGGA | 2 cut(s) | 913, 928 |
| TspRI | CASTG | 4 cut(s) | 91, 367, 765, 1166 |
| VpaK11BI | GGWCC | 4 cut(s) | 766, 979, 988, 1278 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 773 |
| XapI | RAATTY | 1 cut(s) | 481 |
| XceI | RCATGY | 1 cut(s) | 260 |
| XhoI | CTCGAG | 1 cut(s) | 1079 |
| XspI | CTAG | 2 cut(s) | 164, 936 |
| Zsp2I | ATGCAT | 1 cut(s) | 258 |