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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 230, 282 |
| AciI | CCGC | 6 cut(s) | 38, 48, 89, 164, 182, 254 |
| AclI | AACGTT | 1 cut(s) | 78 |
| AclWI | GGATC | 1 cut(s) | 154 |
| AcoI | YGGCCR | 1 cut(s) | 324 |
| AfaI | GTAC | 2 cut(s) | 106, 133 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 265 |
| AgsI | TTSAA | 1 cut(s) | 115 |
| AjnI | CCWGG | 2 cut(s) | 193, 272 |
| AluBI | AGCT | 1 cut(s) | 292 |
| AluI | AGCT | 1 cut(s) | 292 |
| Alw21I | GWGCWC | 1 cut(s) | 294 |
| Alw26I | GTCTC | 1 cut(s) | 302 |
| AlwI | GGATC | 1 cut(s) | 154 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 207 |
| AoxI | GGCC | 1 cut(s) | 324 |
| ApeKI | GCWGC | 1 cut(s) | 207 |
| AspLEI | GCGC | 2 cut(s) | 232, 282 |
| AspS9I | GGNCC | 2 cut(s) | 40, 256 |
| AvaII | GGWCC | 2 cut(s) | 40, 256 |
| BalI | TGGCCA | 1 cut(s) | 326 |
| BanII | GRGCYC | 1 cut(s) | 294 |
| Bbv12I | GWGCWC | 1 cut(s) | 294 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 194 |
| BciT130I | CCWGG | 2 cut(s) | 195, 274 |
| BcoDI | GTCTC | 1 cut(s) | 302 |
| BfaI | CTAG | 1 cut(s) | 86 |
| BisI | GCNGC | 1 cut(s) | 208 |
| BlsI | GCNGC | 1 cut(s) | 209 |
| Bme1390I | CCNGG | 2 cut(s) | 195, 274 |
| Bme18I | GGWCC | 2 cut(s) | 40, 256 |
| BmgT120I | GGNCC | 2 cut(s) | 40, 256 |
| BmiI | GGNNCC | 1 cut(s) | 141 |
| BmrFI | CCNGG | 2 cut(s) | 195, 274 |
| BmsI | GCATC | 1 cut(s) | 175 |
| Bpu10I | CCTNAGC | 2 cut(s) | 27, 293 |
| BsaI | GGTCTC | 1 cut(s) | 302 |
| BsaJI | CCNNGG | 2 cut(s) | 135, 273 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 265 |
| Bse1I | ACTGG | 3 cut(s) | 249, 255, 369 |
| BseBI | CCWGG | 2 cut(s) | 195, 274 |
| BseDI | CCNNGG | 2 cut(s) | 135, 273 |
| BseGI | GGATG | 1 cut(s) | 190 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 265 |
| BseMII | CTCAG | 4 cut(s) | 18, 134, 297, 307 |
| BseNI | ACTGG | 3 cut(s) | 249, 255, 369 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 1 cut(s) | 194 |
| BseYI | CCCAGC | 1 cut(s) | 382 |
| Bsh1236I | CGCG | 2 cut(s) | 230, 282 |
| BshFI | GGCC | 1 cut(s) | 326 |
| BsiHKAI | GWGCWC | 1 cut(s) | 294 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 265 |
| BsmAI | GTCTC | 1 cut(s) | 302 |
| BsnI | GGCC | 1 cut(s) | 326 |
| Bso31I | GGTCTC | 1 cut(s) | 302 |
| Bsp1286I | GDGCHC | 1 cut(s) | 294 |
| Bsp143I | GATC | 2 cut(s) | 123, 159 |
| BspACI | CCGC | 6 cut(s) | 38, 48, 89, 164, 182, 254 |
| BspANI | GGCC | 1 cut(s) | 326 |
| BspCNI | CTCAG | 4 cut(s) | 19, 133, 298, 306 |
| BspFNI | CGCG | 2 cut(s) | 230, 282 |
| BspLI | GGNNCC | 1 cut(s) | 141 |
| BspPI | GGATC | 1 cut(s) | 154 |
| BspTNI | GGTCTC | 1 cut(s) | 302 |
| BsrI | ACTGG | 3 cut(s) | 249, 255, 369 |
| BssECI | CCNNGG | 2 cut(s) | 135, 273 |
| BssMI | GATC | 2 cut(s) | 123, 159 |
| Bst2UI | CCWGG | 2 cut(s) | 195, 274 |
| Bst4CI | ACNGT | 5 cut(s) | 11, 104, 131, 136, 203 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 212, 284 |
| BstDEI | CTNAG | 4 cut(s) | 27, 120, 293, 306 |
| BstDSI | CCRYGG | 1 cut(s) | 135 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 1 cut(s) | 190 |
| BstFNI | CGCG | 2 cut(s) | 230, 282 |
| BstHHI | GCGC | 2 cut(s) | 232, 282 |
| BstKTI | GATC | 2 cut(s) | 126, 162 |
| BstMAI | GTCTC | 1 cut(s) | 302 |
| BstMBI | GATC | 2 cut(s) | 123, 159 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 204, 368, 383 |
| BstNI | CCWGG | 2 cut(s) | 195, 274 |
| BstSCI | CCNGG | 2 cut(s) | 193, 272 |
| BstUI | CGCG | 2 cut(s) | 230, 282 |
| BstV1I | GCAGC | 1 cut(s) | 194 |
| BstX2I | RGATCY | 1 cut(s) | 159 |
| BstYI | RGATCY | 1 cut(s) | 159 |
| BsuRI | GGCC | 1 cut(s) | 326 |
| BtgI | CCRYGG | 1 cut(s) | 135 |
| BtsCI | GGATG | 1 cut(s) | 190 |
| BtsIMutI | CAGTG | 2 cut(s) | 248, 362 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 212, 284 |
| CaiI | CAGNNNCTG | 1 cut(s) | 207 |
| CfoI | GCGC | 2 cut(s) | 232, 282 |
| Cfr13I | GGNCC | 2 cut(s) | 40, 256 |
| Csp6I | GTAC | 2 cut(s) | 105, 132 |
| CviAII | CATG | 1 cut(s) | 221 |
| CviJI | RGCY | 6 cut(s) | 142, 149, 214, 292, 305, 326 |
| CviKI_1 | RGCY | 6 cut(s) | 142, 149, 214, 292, 305, 326 |
| CviQI | GTAC | 2 cut(s) | 105, 132 |
| DdeI | CTNAG | 4 cut(s) | 27, 120, 293, 306 |
| DpnI | GATC | 2 cut(s) | 125, 161 |
| DpnII | GATC | 2 cut(s) | 123, 159 |
| EaeI | YGGCCR | 1 cut(s) | 324 |
| EciI | GGCGGA | 2 cut(s) | 37, 269 |
| Ecl136II | GAGCTC | 1 cut(s) | 292 |
| Eco24I | GRGCYC | 1 cut(s) | 294 |
| Eco31I | GGTCTC | 1 cut(s) | 302 |
| Eco47I | GGWCC | 2 cut(s) | 40, 256 |
| Eco53kI | GAGCTC | 1 cut(s) | 292 |
| EcoICRI | GAGCTC | 1 cut(s) | 292 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoRII | CCWGG | 2 cut(s) | 193, 272 |
| EcoT38I | GRGCYC | 1 cut(s) | 294 |
| FaeI | CATG | 1 cut(s) | 224 |
| FaiI | YATR | 6 cut(s) | 168, 218, 222, 236, 240, 388 |
| FatI | CATG | 1 cut(s) | 220 |
| FauI | CCCGC | 1 cut(s) | 171 |
| Fnu4HI | GCNGC | 1 cut(s) | 208 |
| FokI | GGATG | 1 cut(s) | 197 |
| FriOI | GRGCYC | 1 cut(s) | 294 |
| Fsp4HI | GCNGC | 1 cut(s) | 208 |
| FspBI | CTAG | 1 cut(s) | 86 |
| GlaI | GCGC | 2 cut(s) | 231, 281 |
| GluI | GCNGC | 1 cut(s) | 208 |
| GsaI | CCCAGC | 1 cut(s) | 386 |
| HaeIII | GGCC | 1 cut(s) | 326 |
| HhaI | GCGC | 2 cut(s) | 232, 282 |
| Hin1II | CATG | 1 cut(s) | 224 |
| Hin6I | GCGC | 2 cut(s) | 230, 280 |
| HinP1I | GCGC | 2 cut(s) | 230, 280 |
| HincII | GTYRAC | 2 cut(s) | 82, 94 |
| HindII | GTYRAC | 2 cut(s) | 82, 94 |
| HpaI | GTTAAC | 1 cut(s) | 82 |
| Hpy166II | GTNNAC | 3 cut(s) | 82, 94, 107 |
| Hpy188I | TCNGA | 1 cut(s) | 123 |
| Hpy8I | GTNNAC | 3 cut(s) | 82, 94, 107 |
| HpyAV | CCTTC | 1 cut(s) | 269 |
| HpyCH4III | ACNGT | 5 cut(s) | 11, 104, 131, 136, 203 |
| HpyCH4IV | ACGT | 1 cut(s) | 78 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 204, 368, 383 |
| HpyF3I | CTNAG | 4 cut(s) | 27, 120, 293, 306 |
| HpySE526I | ACGT | 1 cut(s) | 78 |
| Hsp92II | CATG | 1 cut(s) | 224 |
| HspAI | GCGC | 2 cut(s) | 230, 280 |
| KspAI | GTTAAC | 1 cut(s) | 82 |
| Kzo9I | GATC | 2 cut(s) | 123, 159 |
| LmnI | GCTCC | 4 cut(s) | 31, 139, 277, 289 |
| Lsp1109I | GCAGC | 1 cut(s) | 194 |
| LweI | GCATC | 1 cut(s) | 175 |
| MaeI | CTAG | 1 cut(s) | 86 |
| MaeII | ACGT | 1 cut(s) | 78 |
| MalI | GATC | 2 cut(s) | 125, 161 |
| MboI | GATC | 2 cut(s) | 123, 159 |
| MflI | RGATCY | 1 cut(s) | 159 |
| MhlI | GDGCHC | 1 cut(s) | 294 |
| MlsI | TGGCCA | 1 cut(s) | 326 |
| MluNI | TGGCCA | 1 cut(s) | 326 |
| MnlI | CCTC | 2 cut(s) | 22, 236 |
| Mox20I | TGGCCA | 1 cut(s) | 326 |
| MscI | TGGCCA | 1 cut(s) | 326 |
| MseI | TTAA | 2 cut(s) | 81, 99 |
| MslI | CAYNNNNRTG | 1 cut(s) | 332 |
| Msp20I | TGGCCA | 1 cut(s) | 326 |
| MspA1I | CMGCKG | 1 cut(s) | 182 |
| MspR9I | CCNGG | 2 cut(s) | 195, 274 |
| MvaI | CCWGG | 2 cut(s) | 195, 274 |
| MvnI | CGCG | 2 cut(s) | 230, 282 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 204, 368, 383 |
| NdeII | GATC | 2 cut(s) | 123, 159 |
| NlaIII | CATG | 1 cut(s) | 224 |
| NlaIV | GGNNCC | 1 cut(s) | 141 |
| PkrI | GCNGC | 1 cut(s) | 209 |
| Psp124BI | GAGCTC | 1 cut(s) | 294 |
| Psp1406I | AACGTT | 1 cut(s) | 78 |
| Psp6I | CCWGG | 2 cut(s) | 193, 272 |
| PspFI | CCCAGC | 1 cut(s) | 382 |
| PspGI | CCWGG | 2 cut(s) | 193, 272 |
| PspN4I | GGNNCC | 1 cut(s) | 141 |
| PspPI | GGNCC | 2 cut(s) | 40, 256 |
| PstNI | CAGNNNCTG | 1 cut(s) | 207 |
| PsuI | RGATCY | 1 cut(s) | 159 |
| RsaI | GTAC | 2 cut(s) | 106, 133 |
| RsaNI | GTAC | 2 cut(s) | 105, 132 |
| RseI | CAYNNNNRTG | 1 cut(s) | 332 |
| SacI | GAGCTC | 1 cut(s) | 294 |
| SaqAI | TTAA | 2 cut(s) | 81, 99 |
| SatI | GCNGC | 1 cut(s) | 208 |
| Sau3AI | GATC | 2 cut(s) | 123, 159 |
| Sau96I | GGNCC | 2 cut(s) | 40, 256 |
| ScrFI | CCNGG | 2 cut(s) | 195, 274 |
| SduI | GDGCHC | 1 cut(s) | 294 |
| SetI | ASST | 6 cut(s) | 81, 87, 228, 261, 294, 344 |
| SfaNI | GCATC | 1 cut(s) | 175 |
| SinI | GGWCC | 2 cut(s) | 40, 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 332 |
| SsiI | CCGC | 6 cut(s) | 38, 48, 89, 164, 182, 254 |
| SspMI | CTAG | 1 cut(s) | 86 |
| SstI | GAGCTC | 1 cut(s) | 294 |
| StyD4I | CCNGG | 2 cut(s) | 193, 272 |
| TaaI | ACNGT | 5 cut(s) | 11, 104, 131, 136, 203 |
| TaiI | ACGT | 1 cut(s) | 81 |
| TaqI | TCGA | 1 cut(s) | 345 |
| Tru1I | TTAA | 2 cut(s) | 81, 99 |
| Tru9I | TTAA | 2 cut(s) | 81, 99 |
| TscAI | CASTG | 2 cut(s) | 255, 369 |
| TseI | GCWGC | 1 cut(s) | 207 |
| TspDTI | ATGAA | 1 cut(s) | 255 |
| TspRI | CASTG | 2 cut(s) | 255, 369 |
| VpaK11BI | GGWCC | 2 cut(s) | 40, 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XspI | CTAG | 1 cut(s) | 86 |