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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 186, 290 |
| AccIII | TCCGGA | 1 cut(s) | 124 |
| AciI | CCGC | 6 cut(s) | 63, 72, 92, 135, 158, 288 |
| AclWI | GGATC | 3 cut(s) | 48, 135, 168 |
| AcoI | YGGCCR | 1 cut(s) | 228 |
| AcsI | RAATTY | 1 cut(s) | 245 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AgsI | TTSAA | 2 cut(s) | 118, 259 |
| AluBI | AGCT | 3 cut(s) | 77, 148, 268 |
| AluI | AGCT | 3 cut(s) | 77, 148, 268 |
| Alw21I | GWGCWC | 1 cut(s) | 112 |
| AlwI | GGATC | 3 cut(s) | 48, 135, 168 |
| Aor13HI | TCCGGA | 1 cut(s) | 124 |
| AoxI | GGCC | 2 cut(s) | 213, 228 |
| ApeKI | GCWGC | 1 cut(s) | 74 |
| ApoI | RAATTY | 1 cut(s) | 245 |
| AspLEI | GCGC | 3 cut(s) | 186, 198, 292 |
| AspS9I | GGNCC | 1 cut(s) | 214 |
| BalI | TGGCCA | 1 cut(s) | 230 |
| Bbv12I | GWGCWC | 1 cut(s) | 112 |
| BbvI | GCAGC | 1 cut(s) | 86 |
| BccI | CCATC | 1 cut(s) | 66 |
| BisI | GCNGC | 2 cut(s) | 75, 135 |
| BlsI | GCNGC | 2 cut(s) | 76, 136 |
| BmgT120I | GGNCC | 1 cut(s) | 214 |
| BpmI | CTGGAG | 1 cut(s) | 290 |
| Bpu10I | CCTNAGC | 1 cut(s) | 210 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| BseAI | TCCGGA | 1 cut(s) | 124 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseXI | GCAGC | 1 cut(s) | 86 |
| BseYI | CCCAGC | 1 cut(s) | 88 |
| Bsh1236I | CGCG | 2 cut(s) | 186, 290 |
| BshFI | GGCC | 2 cut(s) | 215, 230 |
| BsiHKAI | GWGCWC | 1 cut(s) | 112 |
| BsiSI | CCGG | 1 cut(s) | 125 |
| BsnI | GGCC | 2 cut(s) | 215, 230 |
| Bsp1286I | GDGCHC | 1 cut(s) | 112 |
| Bsp13I | TCCGGA | 1 cut(s) | 124 |
| Bsp143I | GATC | 3 cut(s) | 40, 127, 160 |
| BspACI | CCGC | 6 cut(s) | 63, 72, 92, 135, 158, 288 |
| BspANI | GGCC | 2 cut(s) | 215, 230 |
| BspEI | TCCGGA | 1 cut(s) | 124 |
| BspFNI | CGCG | 2 cut(s) | 186, 290 |
| BspPI | GGATC | 3 cut(s) | 48, 135, 168 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BssMI | GATC | 3 cut(s) | 40, 127, 160 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 254 |
| BstC8I | GCNNGC | 1 cut(s) | 150 |
| BstDEI | CTNAG | 1 cut(s) | 210 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 2 cut(s) | 186, 290 |
| BstHHI | GCGC | 3 cut(s) | 186, 198, 292 |
| BstKTI | GATC | 3 cut(s) | 43, 130, 163 |
| BstMBI | GATC | 3 cut(s) | 40, 127, 160 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 71, 287, 298 |
| BstUI | CGCG | 2 cut(s) | 186, 290 |
| BstV1I | GCAGC | 1 cut(s) | 86 |
| BstX2I | RGATCY | 2 cut(s) | 127, 160 |
| BstYI | RGATCY | 2 cut(s) | 127, 160 |
| BsuRI | GGCC | 2 cut(s) | 215, 230 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| Cac8I | GCNNGC | 1 cut(s) | 150 |
| CfoI | GCGC | 3 cut(s) | 186, 198, 292 |
| Cfr13I | GGNCC | 1 cut(s) | 214 |
| CviJI | RGCY | 6 cut(s) | 77, 148, 209, 215, 230, 268 |
| CviKI_1 | RGCY | 6 cut(s) | 77, 148, 209, 215, 230, 268 |
| DdeI | CTNAG | 1 cut(s) | 210 |
| DpnI | GATC | 3 cut(s) | 42, 129, 162 |
| DpnII | GATC | 3 cut(s) | 40, 127, 160 |
| EaeI | YGGCCR | 1 cut(s) | 228 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 254 |
| EarI | CTCTTC | 2 cut(s) | 24, 254 |
| EciI | GGCGGA | 1 cut(s) | 173 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| FaiI | YATR | 3 cut(s) | 140, 144, 252 |
| FauI | CCCGC | 3 cut(s) | 70, 79, 295 |
| Fnu4HI | GCNGC | 2 cut(s) | 75, 135 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 2 cut(s) | 75, 135 |
| GlaI | GCGC | 3 cut(s) | 185, 197, 291 |
| GluI | GCNGC | 2 cut(s) | 75, 135 |
| GsaI | CCCAGC | 1 cut(s) | 92 |
| GsuI | CTGGAG | 1 cut(s) | 290 |
| HaeIII | GGCC | 2 cut(s) | 215, 230 |
| HapII | CCGG | 1 cut(s) | 125 |
| HhaI | GCGC | 3 cut(s) | 186, 198, 292 |
| Hin6I | GCGC | 3 cut(s) | 184, 196, 290 |
| HinP1I | GCGC | 3 cut(s) | 184, 196, 290 |
| HincII | GTYRAC | 1 cut(s) | 103 |
| HindII | GTYRAC | 1 cut(s) | 103 |
| HpaII | CCGG | 1 cut(s) | 125 |
| Hpy166II | GTNNAC | 1 cut(s) | 103 |
| Hpy188III | TCNNGA | 1 cut(s) | 125 |
| Hpy8I | GTNNAC | 1 cut(s) | 103 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 71, 287, 298 |
| HpyF3I | CTNAG | 1 cut(s) | 210 |
| HspAI | GCGC | 3 cut(s) | 184, 196, 290 |
| Kpn2I | TCCGGA | 1 cut(s) | 124 |
| Kzo9I | GATC | 3 cut(s) | 40, 127, 160 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 181, 265 |
| LpnPI | CCDG | 4 cut(s) | 102, 134, 138, 254 |
| Lsp1109I | GCAGC | 1 cut(s) | 86 |
| MalI | GATC | 3 cut(s) | 42, 129, 162 |
| MboI | GATC | 3 cut(s) | 40, 127, 160 |
| MboII | GAAGA | 2 cut(s) | 41, 271 |
| MflI | RGATCY | 2 cut(s) | 127, 160 |
| MhlI | GDGCHC | 1 cut(s) | 112 |
| MlsI | TGGCCA | 1 cut(s) | 230 |
| MluCI | AATT | 1 cut(s) | 245 |
| MluNI | TGGCCA | 1 cut(s) | 230 |
| MnlI | CCTC | 3 cut(s) | 8, 255, 265 |
| Mox20I | TGGCCA | 1 cut(s) | 230 |
| MroI | TCCGGA | 1 cut(s) | 124 |
| MscI | TGGCCA | 1 cut(s) | 230 |
| MseI | TTAA | 2 cut(s) | 84, 96 |
| MslI | CAYNNNNRTG | 1 cut(s) | 236 |
| Msp20I | TGGCCA | 1 cut(s) | 230 |
| MspA1I | CMGCKG | 1 cut(s) | 92 |
| MspI | CCGG | 1 cut(s) | 125 |
| MvnI | CGCG | 2 cut(s) | 186, 290 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 71, 287, 298 |
| NdeII | GATC | 3 cut(s) | 40, 127, 160 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 2 cut(s) | 76, 136 |
| PspFI | CCCAGC | 1 cut(s) | 88 |
| PspPI | GGNCC | 1 cut(s) | 214 |
| PsuI | RGATCY | 2 cut(s) | 127, 160 |
| RseI | CAYNNNNRTG | 1 cut(s) | 236 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 2 cut(s) | 84, 96 |
| SatI | GCNGC | 2 cut(s) | 75, 135 |
| Sau3AI | GATC | 3 cut(s) | 40, 127, 160 |
| Sau96I | GGNCC | 1 cut(s) | 214 |
| SduI | GDGCHC | 1 cut(s) | 112 |
| SetI | ASST | 4 cut(s) | 49, 79, 150, 270 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 236 |
| Sse9I | AATT | 1 cut(s) | 245 |
| SsiI | CCGC | 6 cut(s) | 63, 72, 92, 135, 158, 288 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TasI | AATT | 1 cut(s) | 245 |
| TauI | GCSGC | 1 cut(s) | 137 |
| Tru1I | TTAA | 2 cut(s) | 84, 96 |
| Tru9I | TTAA | 2 cut(s) | 84, 96 |
| TseI | GCWGC | 1 cut(s) | 74 |
| XapI | RAATTY | 1 cut(s) | 245 |