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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 175, 621 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 58, 334 |
| AccBSI | CCGCTC | 1 cut(s) | 578 |
| AccII | CGCG | 1 cut(s) | 418 |
| AclWI | GGATC | 2 cut(s) | 677, 690 |
| AcoI | YGGCCR | 1 cut(s) | 339 |
| AcsI | RAATTY | 1 cut(s) | 356 |
| AcuI | CTGAAG | 1 cut(s) | 389 |
| AcyI | GRCGYC | 2 cut(s) | 176, 622 |
| AfaI | GTAC | 2 cut(s) | 254, 436 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 58, 185, 334, 572 |
| AgsI | TTSAA | 2 cut(s) | 68, 529 |
| AjiI | CACGTC | 1 cut(s) | 655 |
| AjnI | CCWGG | 2 cut(s) | 657, 678 |
| AluBI | AGCT | 9 cut(s) | 49, 58, 108, 259, 295, 334, 367, 518, 739 |
| AluI | AGCT | 9 cut(s) | 49, 58, 108, 259, 295, 334, 367, 518, 739 |
| Alw21I | GWGCWC | 2 cut(s) | 425, 502 |
| Alw26I | GTCTC | 3 cut(s) | 162, 547, 609 |
| AlwI | GGATC | 2 cut(s) | 677, 690 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 58 |
| AoxI | GGCC | 2 cut(s) | 318, 339 |
| ApeKI | GCWGC | 1 cut(s) | 739 |
| ApoI | RAATTY | 1 cut(s) | 356 |
| AspLEI | GCGC | 4 cut(s) | 178, 379, 624, 632 |
| AspS9I | GGNCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| AsuHPI | GGTGA | 2 cut(s) | 109, 665 |
| AvaII | GGWCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| BalI | TGGCCA | 1 cut(s) | 341 |
| BamHI | GGATCC | 1 cut(s) | 682 |
| BanI | GGYRCC | 2 cut(s) | 175, 621 |
| BauI | CACGAG | 1 cut(s) | 501 |
| Bbv12I | GWGCWC | 2 cut(s) | 425, 502 |
| BbvI | GCAGC | 1 cut(s) | 726 |
| BccI | CCATC | 3 cut(s) | 222, 543, 702 |
| BceAI | ACGGC | 1 cut(s) | 163 |
| BciT130I | CCWGG | 2 cut(s) | 659, 680 |
| BcoDI | GTCTC | 3 cut(s) | 162, 547, 609 |
| BfaI | CTAG | 5 cut(s) | 137, 266, 296, 591, 609 |
| BfmI | CTRYAG | 1 cut(s) | 456 |
| BfoI | RGCGCY | 4 cut(s) | 179, 380, 625, 633 |
| BisI | GCNGC | 2 cut(s) | 17, 740 |
| BlsI | GCNGC | 2 cut(s) | 18, 741 |
| Bme1390I | CCNGG | 2 cut(s) | 659, 680 |
| Bme18I | GGWCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| BmgBI | CACGTC | 1 cut(s) | 655 |
| BmgT120I | GGNCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| BmiI | GGNNCC | 6 cut(s) | 177, 190, 469, 545, 623, 684 |
| BmrFI | CCNGG | 2 cut(s) | 659, 680 |
| BmsI | GCATC | 3 cut(s) | 541, 571, 715 |
| BpmI | CTGGAG | 2 cut(s) | 35, 594 |
| BpuEI | CTTGAG | 1 cut(s) | 144 |
| BsaHI | GRCGYC | 2 cut(s) | 176, 622 |
| BsaJI | CCNNGG | 1 cut(s) | 658 |
| BsaWI | WCCGGW | 1 cut(s) | 449 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 58, 185, 334, 572 |
| Bse1I | ACTGG | 1 cut(s) | 577 |
| BseBI | CCWGG | 2 cut(s) | 659, 680 |
| BseDI | CCNNGG | 1 cut(s) | 658 |
| BseGI | GGATG | 4 cut(s) | 207, 532, 586, 694 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 58, 185, 334, 572 |
| BseNI | ACTGG | 1 cut(s) | 577 |
| BseRI | GAGGAG | 1 cut(s) | 466 |
| BseXI | GCAGC | 1 cut(s) | 726 |
| BseYI | CCCAGC | 2 cut(s) | 58, 626 |
| Bsh1236I | CGCG | 1 cut(s) | 418 |
| BshFI | GGCC | 2 cut(s) | 320, 341 |
| BshNI | GGYRCC | 2 cut(s) | 175, 621 |
| BsiHKAI | GWGCWC | 2 cut(s) | 425, 502 |
| BsiSI | CCGG | 1 cut(s) | 450 |
| BslFI | GGGAC | 2 cut(s) | 64, 481 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 58, 185, 334, 572 |
| BsmAI | GTCTC | 3 cut(s) | 162, 547, 609 |
| BsmFI | GGGAC | 2 cut(s) | 64, 481 |
| BsnI | GGCC | 2 cut(s) | 320, 341 |
| Bsp1286I | GDGCHC | 2 cut(s) | 425, 502 |
| Bsp143I | GATC | 2 cut(s) | 102, 682 |
| BspANI | GGCC | 2 cut(s) | 320, 341 |
| BspFNI | CGCG | 1 cut(s) | 418 |
| BspLI | GGNNCC | 6 cut(s) | 177, 190, 469, 545, 623, 684 |
| BspPI | GGATC | 2 cut(s) | 677, 690 |
| BspT107I | GGYRCC | 2 cut(s) | 175, 621 |
| BsrBI | CCGCTC | 1 cut(s) | 578 |
| BsrI | ACTGG | 1 cut(s) | 577 |
| BssECI | CCNNGG | 1 cut(s) | 658 |
| BssMI | GATC | 2 cut(s) | 102, 682 |
| BssNI | GRCGYC | 2 cut(s) | 176, 622 |
| BssSI | CACGAG | 1 cut(s) | 501 |
| Bst2BI | CACGAG | 1 cut(s) | 501 |
| Bst2UI | CCWGG | 2 cut(s) | 659, 680 |
| Bst6I | CTCTTC | 1 cut(s) | 654 |
| BstACI | GRCGYC | 2 cut(s) | 176, 622 |
| BstC8I | GCNNGC | 3 cut(s) | 261, 691, 709 |
| BstDEI | CTNAG | 1 cut(s) | 153 |
| BstF5I | GGATG | 4 cut(s) | 207, 532, 586, 694 |
| BstFNI | CGCG | 1 cut(s) | 418 |
| BstH2I | RGCGCY | 4 cut(s) | 179, 380, 625, 633 |
| BstHHI | GCGC | 4 cut(s) | 178, 379, 624, 632 |
| BstKTI | GATC | 2 cut(s) | 105, 685 |
| BstMAI | GTCTC | 3 cut(s) | 162, 547, 609 |
| BstMBI | GATC | 2 cut(s) | 102, 682 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 55, 383 |
| BstNI | CCWGG | 2 cut(s) | 659, 680 |
| BstNSI | RCATGY | 1 cut(s) | 693 |
| BstSCI | CCNGG | 2 cut(s) | 657, 678 |
| BstSFI | CTRYAG | 1 cut(s) | 456 |
| BstUI | CGCG | 1 cut(s) | 418 |
| BstV1I | GCAGC | 1 cut(s) | 726 |
| BstX2I | RGATCY | 1 cut(s) | 682 |
| BstYI | RGATCY | 1 cut(s) | 682 |
| BsuRI | GGCC | 2 cut(s) | 320, 341 |
| BtrI | CACGTC | 1 cut(s) | 655 |
| BtsCI | GGATG | 4 cut(s) | 207, 532, 586, 694 |
| Cac8I | GCNNGC | 3 cut(s) | 261, 691, 709 |
| CaiI | CAGNNNCTG | 1 cut(s) | 58 |
| CfoI | GCGC | 4 cut(s) | 178, 379, 624, 632 |
| Cfr13I | GGNCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| CseI | GACGC | 1 cut(s) | 713 |
| Csp6I | GTAC | 2 cut(s) | 253, 435 |
| CviAII | CATG | 3 cut(s) | 94, 690, 743 |
| CviQI | GTAC | 2 cut(s) | 253, 435 |
| DdeI | CTNAG | 1 cut(s) | 153 |
| DinI | GGCGCC | 2 cut(s) | 177, 623 |
| DpnI | GATC | 2 cut(s) | 104, 684 |
| DpnII | GATC | 2 cut(s) | 102, 682 |
| EaeI | YGGCCR | 1 cut(s) | 339 |
| Eam1104I | CTCTTC | 1 cut(s) | 654 |
| EarI | CTCTTC | 1 cut(s) | 654 |
| EciI | GGCGGA | 2 cut(s) | 522, 687 |
| Eco47I | GGWCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| Eco57I | CTGAAG | 1 cut(s) | 389 |
| EcoO109I | RGGNCCY | 1 cut(s) | 543 |
| EcoRII | CCWGG | 2 cut(s) | 657, 678 |
| EgeI | GGCGCC | 2 cut(s) | 177, 623 |
| EheI | GGCGCC | 2 cut(s) | 177, 623 |
| FaeI | CATG | 3 cut(s) | 97, 693, 746 |
| FaiI | YATR | 6 cut(s) | 95, 123, 251, 458, 691, 744 |
| FaqI | GGGAC | 2 cut(s) | 64, 481 |
| FatI | CATG | 3 cut(s) | 93, 689, 742 |
| Fnu4HI | GCNGC | 2 cut(s) | 17, 740 |
| FokI | GGATG | 4 cut(s) | 194, 519, 593, 681 |
| Fsp4HI | GCNGC | 2 cut(s) | 17, 740 |
| FspBI | CTAG | 5 cut(s) | 137, 266, 296, 591, 609 |
| GlaI | GCGC | 4 cut(s) | 177, 378, 623, 631 |
| GluI | GCNGC | 2 cut(s) | 17, 740 |
| GsaI | CCCAGC | 2 cut(s) | 62, 630 |
| GsuI | CTGGAG | 2 cut(s) | 35, 594 |
| HaeII | RGCGCY | 4 cut(s) | 179, 380, 625, 633 |
| HaeIII | GGCC | 2 cut(s) | 320, 341 |
| HapII | CCGG | 1 cut(s) | 450 |
| HgaI | GACGC | 1 cut(s) | 713 |
| HhaI | GCGC | 4 cut(s) | 178, 379, 624, 632 |
| Hin1I | GRCGYC | 2 cut(s) | 176, 622 |
| Hin1II | CATG | 3 cut(s) | 97, 693, 746 |
| Hin6I | GCGC | 4 cut(s) | 176, 377, 622, 630 |
| HinP1I | GCGC | 4 cut(s) | 176, 377, 622, 630 |
| HpaII | CCGG | 1 cut(s) | 450 |
| HphI | GGTGA | 2 cut(s) | 109, 665 |
| Hpy188I | TCNGA | 1 cut(s) | 45 |
| Hpy188III | TCNNGA | 1 cut(s) | 52 |
| Hpy99I | CGWCG | 1 cut(s) | 707 |
| HpyAV | CCTTC | 3 cut(s) | 121, 284, 367 |
| HpyCH4IV | ACGT | 1 cut(s) | 654 |
| HpyCH4V | TGCA | 3 cut(s) | 584, 689, 742 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 55, 383 |
| HpyF3I | CTNAG | 1 cut(s) | 153 |
| HpySE526I | ACGT | 1 cut(s) | 654 |
| Hsp92I | GRCGYC | 2 cut(s) | 176, 622 |
| Hsp92II | CATG | 3 cut(s) | 97, 693, 746 |
| HspAI | GCGC | 4 cut(s) | 176, 377, 622, 630 |
| KasI | GGCGCC | 2 cut(s) | 175, 621 |
| Kzo9I | GATC | 2 cut(s) | 102, 682 |
| LmnI | GCTCC | 5 cut(s) | 54, 113, 420, 497, 575 |
| Lsp1109I | GCAGC | 1 cut(s) | 726 |
| LweI | GCATC | 3 cut(s) | 541, 571, 715 |
| MaeI | CTAG | 5 cut(s) | 137, 266, 296, 591, 609 |
| MaeII | ACGT | 1 cut(s) | 654 |
| MalI | GATC | 2 cut(s) | 104, 684 |
| MbiI | CCGCTC | 1 cut(s) | 578 |
| MboI | GATC | 2 cut(s) | 102, 682 |
| MboII | GAAGA | 3 cut(s) | 112, 382, 641 |
| MflI | RGATCY | 1 cut(s) | 682 |
| MhlI | GDGCHC | 2 cut(s) | 425, 502 |
| MlsI | TGGCCA | 1 cut(s) | 341 |
| MluCI | AATT | 1 cut(s) | 356 |
| MluNI | TGGCCA | 1 cut(s) | 341 |
| Mly113I | GGCGCC | 2 cut(s) | 176, 622 |
| MmeI | TCCRAC | 1 cut(s) | 475 |
| MnlI | CCTC | 6 cut(s) | 125, 251, 256, 484, 487, 497 |
| Mox20I | TGGCCA | 1 cut(s) | 341 |
| MscI | TGGCCA | 1 cut(s) | 341 |
| MseI | TTAA | 2 cut(s) | 132, 167 |
| MslI | CAYNNNNRTG | 1 cut(s) | 347 |
| Msp20I | TGGCCA | 1 cut(s) | 341 |
| MspA1I | CMGCKG | 2 cut(s) | 440, 739 |
| MspI | CCGG | 1 cut(s) | 450 |
| MspR9I | CCNGG | 2 cut(s) | 659, 680 |
| MvaI | CCWGG | 2 cut(s) | 659, 680 |
| MvnI | CGCG | 1 cut(s) | 418 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 55, 383 |
| NarI | GGCGCC | 2 cut(s) | 176, 622 |
| NdeII | GATC | 2 cut(s) | 102, 682 |
| NlaIII | CATG | 3 cut(s) | 97, 693, 746 |
| NlaIV | GGNNCC | 6 cut(s) | 177, 190, 469, 545, 623, 684 |
| NspI | RCATGY | 1 cut(s) | 693 |
| PaeI | GCATGC | 1 cut(s) | 693 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 58, 334 |
| PkrI | GCNGC | 2 cut(s) | 18, 741 |
| PluTI | GGCGCC | 2 cut(s) | 179, 625 |
| PpuMI | RGGWCCY | 1 cut(s) | 543 |
| Psp5II | RGGWCCY | 1 cut(s) | 543 |
| Psp6I | CCWGG | 2 cut(s) | 657, 678 |
| PspFI | CCCAGC | 2 cut(s) | 58, 626 |
| PspGI | CCWGG | 2 cut(s) | 657, 678 |
| PspN4I | GGNNCC | 6 cut(s) | 177, 190, 469, 545, 623, 684 |
| PspPI | GGNCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| PspPPI | RGGWCCY | 1 cut(s) | 543 |
| PstNI | CAGNNNCTG | 1 cut(s) | 58 |
| PsuI | RGATCY | 1 cut(s) | 682 |
| PvuII | CAGCTG | 1 cut(s) | 739 |
| RsaI | GTAC | 2 cut(s) | 254, 436 |
| RsaNI | GTAC | 2 cut(s) | 253, 435 |
| RseI | CAYNNNNRTG | 1 cut(s) | 347 |
| SaqAI | TTAA | 2 cut(s) | 132, 167 |
| SatI | GCNGC | 2 cut(s) | 17, 740 |
| Sau3AI | GATC | 2 cut(s) | 102, 682 |
| Sau96I | GGNCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| ScrFI | CCNGG | 2 cut(s) | 659, 680 |
| SduI | GDGCHC | 2 cut(s) | 425, 502 |
| SfaNI | GCATC | 3 cut(s) | 541, 571, 715 |
| SfcI | CTRYAG | 1 cut(s) | 456 |
| SfoI | GGCGCC | 2 cut(s) | 177, 623 |
| SinI | GGWCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 347 |
| SmlI | CTYRAG | 1 cut(s) | 159 |
| SmoI | CTYRAG | 1 cut(s) | 159 |
| SphI | GCATGC | 1 cut(s) | 693 |
| Sse9I | AATT | 1 cut(s) | 356 |
| SspDI | GGCGCC | 2 cut(s) | 175, 621 |
| SspMI | CTAG | 5 cut(s) | 137, 266, 296, 591, 609 |
| StyD4I | CCNGG | 2 cut(s) | 657, 678 |
| TaiI | ACGT | 1 cut(s) | 657 |
| TaqI | TCGA | 1 cut(s) | 87 |
| TasI | AATT | 1 cut(s) | 356 |
| TauI | GCSGC | 1 cut(s) | 19 |
| Tru1I | TTAA | 2 cut(s) | 132, 167 |
| Tru9I | TTAA | 2 cut(s) | 132, 167 |
| TseI | GCWGC | 1 cut(s) | 739 |
| TspDTI | ATGAA | 1 cut(s) | 513 |
| TspGWI | ACGGA | 1 cut(s) | 251 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 58, 334 |
| VpaK11BI | GGWCC | 5 cut(s) | 238, 271, 468, 543, 666 |
| XapI | RAATTY | 1 cut(s) | 356 |
| XceI | RCATGY | 1 cut(s) | 693 |
| XspI | CTAG | 5 cut(s) | 137, 266, 296, 591, 609 |