No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 277 |
| Acc36I | ACCTGC | 2 cut(s) | 124, 277 |
| Acc65I | GGTACC | 2 cut(s) | 109, 136 |
| AccB1I | GGYRCC | 2 cut(s) | 109, 136 |
| AccBSI | CCGCTC | 2 cut(s) | 234, 332 |
| AciI | CCGC | 4 cut(s) | 182, 209, 232, 330 |
| AfaI | GTAC | 2 cut(s) | 111, 138 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 186, 280, 307 |
| AgsI | TTSAA | 2 cut(s) | 22, 145 |
| AjnI | CCWGG | 3 cut(s) | 77, 185, 339 |
| AluBI | AGCT | 3 cut(s) | 157, 286, 358 |
| AluI | AGCT | 3 cut(s) | 157, 286, 358 |
| AoxI | GGCC | 1 cut(s) | 311 |
| ApaI | GGGCCC | 1 cut(s) | 315 |
| Asp718I | GGTACC | 2 cut(s) | 109, 136 |
| AspLEI | GCGC | 3 cut(s) | 31, 55, 84 |
| AspS9I | GGNCC | 4 cut(s) | 116, 169, 311, 312 |
| AsuC2I | CCSGG | 1 cut(s) | 114 |
| AsuNHI | GCTAGC | 1 cut(s) | 286 |
| AvaII | GGWCC | 2 cut(s) | 116, 169 |
| BaeGI | GKGCMC | 1 cut(s) | 315 |
| BanI | GGYRCC | 2 cut(s) | 109, 136 |
| BanII | GRGCYC | 1 cut(s) | 315 |
| BccI | CCATC | 1 cut(s) | 160 |
| BciT130I | CCWGG | 3 cut(s) | 79, 187, 341 |
| BcnI | CCSGG | 1 cut(s) | 114 |
| BfaI | CTAG | 2 cut(s) | 249, 287 |
| BfmI | CTRYAG | 1 cut(s) | 359 |
| BfoI | RGCGCY | 1 cut(s) | 32 |
| BfuAI | ACCTGC | 2 cut(s) | 124, 277 |
| Bme1390I | CCNGG | 4 cut(s) | 79, 114, 187, 341 |
| Bme18I | GGWCC | 2 cut(s) | 116, 169 |
| BmgT120I | GGNCC | 4 cut(s) | 116, 169, 311, 312 |
| BmiI | GGNNCC | 6 cut(s) | 111, 138, 171, 299, 312, 313 |
| BmrFI | CCNGG | 4 cut(s) | 79, 114, 187, 341 |
| BmrI | ACTGGG | 1 cut(s) | 286 |
| BmsI | GCATC | 2 cut(s) | 120, 339 |
| BmtI | GCTAGC | 1 cut(s) | 290 |
| BmuI | ACTGGG | 1 cut(s) | 286 |
| BpuEI | CTTGAG | 1 cut(s) | 287 |
| BpuMI | CCSGG | 1 cut(s) | 114 |
| BsaJI | CCNNGG | 1 cut(s) | 185 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 197, 227 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 186, 280, 307 |
| Bse1I | ACTGG | 2 cut(s) | 292, 318 |
| BseBI | CCWGG | 3 cut(s) | 79, 187, 341 |
| BseDI | CCNNGG | 1 cut(s) | 185 |
| BseGI | GGATG | 2 cut(s) | 171, 354 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 186, 280, 307 |
| BseMII | CTCAG | 1 cut(s) | 246 |
| BseNI | ACTGG | 2 cut(s) | 292, 318 |
| BseSI | GKGCMC | 1 cut(s) | 315 |
| BshFI | GGCC | 1 cut(s) | 313 |
| BshNI | GGYRCC | 2 cut(s) | 109, 136 |
| BsiSI | CCGG | 1 cut(s) | 114 |
| BslFI | GGGAC | 1 cut(s) | 155 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 186, 280, 307 |
| BsmFI | GGGAC | 1 cut(s) | 155 |
| BsnI | GGCC | 1 cut(s) | 313 |
| Bsp120I | GGGCCC | 1 cut(s) | 311 |
| Bsp1286I | GDGCHC | 1 cut(s) | 315 |
| BspACI | CCGC | 4 cut(s) | 182, 209, 232, 330 |
| BspANI | GGCC | 1 cut(s) | 313 |
| BspCNI | CTCAG | 1 cut(s) | 247 |
| BspLI | GGNNCC | 6 cut(s) | 111, 138, 171, 299, 312, 313 |
| BspMI | ACCTGC | 2 cut(s) | 124, 277 |
| BspOI | GCTAGC | 1 cut(s) | 290 |
| BspT107I | GGYRCC | 2 cut(s) | 109, 136 |
| BsrBI | CCGCTC | 2 cut(s) | 234, 332 |
| BsrI | ACTGG | 2 cut(s) | 292, 318 |
| BssECI | CCNNGG | 1 cut(s) | 185 |
| Bst2UI | CCWGG | 3 cut(s) | 79, 187, 341 |
| Bst4CI | ACNGT | 1 cut(s) | 141 |
| BstC8I | GCNNGC | 2 cut(s) | 62, 288 |
| BstDEI | CTNAG | 1 cut(s) | 255 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 305 |
| BstF5I | GGATG | 2 cut(s) | 171, 354 |
| BstH2I | RGCGCY | 1 cut(s) | 32 |
| BstHHI | GCGC | 3 cut(s) | 31, 55, 84 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 35, 50, 61, 81, 88, 245 |
| BstNI | CCWGG | 3 cut(s) | 79, 187, 341 |
| BstSCI | CCNGG | 4 cut(s) | 77, 112, 185, 339 |
| BstSFI | CTRYAG | 1 cut(s) | 359 |
| BstSLI | GKGCMC | 1 cut(s) | 315 |
| BsuRI | GGCC | 1 cut(s) | 313 |
| BtsCI | GGATG | 2 cut(s) | 171, 354 |
| BtsIMutI | CAGTG | 2 cut(s) | 299, 325 |
| BveI | ACCTGC | 2 cut(s) | 124, 277 |
| Cac8I | GCNNGC | 2 cut(s) | 62, 288 |
| CfoI | GCGC | 3 cut(s) | 31, 55, 84 |
| Cfr13I | GGNCC | 4 cut(s) | 116, 169, 311, 312 |
| CseI | GACGC | 1 cut(s) | 332 |
| Csp6I | GTAC | 2 cut(s) | 110, 137 |
| CviJI | RGCY | 8 cut(s) | 91, 157, 190, 248, 286, 290, 313, 358 |
| CviKI_1 | RGCY | 8 cut(s) | 91, 157, 190, 248, 286, 290, 313, 358 |
| CviQI | GTAC | 2 cut(s) | 110, 137 |
| DdeI | CTNAG | 1 cut(s) | 255 |
| EciI | GGCGGA | 1 cut(s) | 224 |
| Eco24I | GRGCYC | 1 cut(s) | 315 |
| Eco47I | GGWCC | 2 cut(s) | 116, 169 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 305 |
| EcoO109I | RGGNCCY | 1 cut(s) | 311 |
| EcoRII | CCWGG | 3 cut(s) | 77, 185, 339 |
| EcoT38I | GRGCYC | 1 cut(s) | 315 |
| FaiI | YATR | 3 cut(s) | 50, 125, 361 |
| FaqI | GGGAC | 1 cut(s) | 155 |
| FauI | CCCGC | 1 cut(s) | 189 |
| FokI | GGATG | 1 cut(s) | 178 |
| FriOI | GRGCYC | 1 cut(s) | 315 |
| FspBI | CTAG | 2 cut(s) | 249, 287 |
| GlaI | GCGC | 3 cut(s) | 30, 54, 83 |
| HaeII | RGCGCY | 1 cut(s) | 32 |
| HaeIII | GGCC | 1 cut(s) | 313 |
| HapII | CCGG | 1 cut(s) | 114 |
| HgaI | GACGC | 1 cut(s) | 332 |
| HhaI | GCGC | 3 cut(s) | 31, 55, 84 |
| Hin6I | GCGC | 3 cut(s) | 29, 53, 82 |
| HinP1I | GCGC | 3 cut(s) | 29, 53, 82 |
| HinfI | GANTC | 1 cut(s) | 212 |
| HpaII | CCGG | 1 cut(s) | 114 |
| HpyAV | CCTTC | 1 cut(s) | 19 |
| HpyCH4III | ACNGT | 1 cut(s) | 141 |
| HpyCH4V | TGCA | 2 cut(s) | 133, 352 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 35, 50, 61, 81, 88, 245 |
| HpyF3I | CTNAG | 1 cut(s) | 255 |
| HspAI | GCGC | 3 cut(s) | 29, 53, 82 |
| KpnI | GGTACC | 2 cut(s) | 113, 140 |
| LmnI | GCTCC | 2 cut(s) | 199, 283 |
| LweI | GCATC | 2 cut(s) | 120, 339 |
| MaeI | CTAG | 2 cut(s) | 249, 287 |
| MaeIII | GTNAC | 2 cut(s) | 213, 320 |
| MbiI | CCGCTC | 2 cut(s) | 234, 332 |
| MboII | GAAGA | 1 cut(s) | 34 |
| MhlI | GDGCHC | 1 cut(s) | 315 |
| MlyI | GAGTC | 1 cut(s) | 221 |
| MmeI | TCCRAC | 1 cut(s) | 177 |
| MnlI | CCTC | 5 cut(s) | 186, 199, 250, 301, 311 |
| MslI | CAYNNNNRTG | 1 cut(s) | 128 |
| MspI | CCGG | 1 cut(s) | 114 |
| MspR9I | CCNGG | 4 cut(s) | 79, 114, 187, 341 |
| MvaI | CCWGG | 3 cut(s) | 79, 187, 341 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 35, 50, 61, 81, 88, 245 |
| NciI | CCSGG | 1 cut(s) | 114 |
| NheI | GCTAGC | 1 cut(s) | 286 |
| NlaIV | GGNNCC | 6 cut(s) | 111, 138, 171, 299, 312, 313 |
| NmeAIII | GCCGAG | 1 cut(s) | 215 |
| NmuCI | GTSAC | 2 cut(s) | 213, 320 |
| PaqCI | CACCTGC | 1 cut(s) | 277 |
| PfoI | TCCNGGA | 1 cut(s) | 339 |
| PleI | GAGTC | 1 cut(s) | 220 |
| PpsI | GAGTC | 1 cut(s) | 220 |
| Psp6I | CCWGG | 3 cut(s) | 77, 185, 339 |
| PspGI | CCWGG | 3 cut(s) | 77, 185, 339 |
| PspN4I | GGNNCC | 6 cut(s) | 111, 138, 171, 299, 312, 313 |
| PspOMI | GGGCCC | 1 cut(s) | 311 |
| PspPI | GGNCC | 4 cut(s) | 116, 169, 311, 312 |
| RsaI | GTAC | 2 cut(s) | 111, 138 |
| RsaNI | GTAC | 2 cut(s) | 110, 137 |
| RseI | CAYNNNNRTG | 1 cut(s) | 128 |
| Sau96I | GGNCC | 4 cut(s) | 116, 169, 311, 312 |
| SchI | GAGTC | 1 cut(s) | 221 |
| ScrFI | CCNGG | 4 cut(s) | 79, 114, 187, 341 |
| SduI | GDGCHC | 1 cut(s) | 315 |
| SetI | ASST | 7 cut(s) | 138, 159, 178, 271, 288, 303, 360 |
| SfaNI | GCATC | 2 cut(s) | 120, 339 |
| SfcI | CTRYAG | 1 cut(s) | 359 |
| SinI | GGWCC | 2 cut(s) | 116, 169 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 128 |
| SmlI | CTYRAG | 1 cut(s) | 302 |
| SmoI | CTYRAG | 1 cut(s) | 302 |
| SsiI | CCGC | 4 cut(s) | 182, 209, 232, 330 |
| SspMI | CTAG | 2 cut(s) | 249, 287 |
| StyD4I | CCNGG | 4 cut(s) | 77, 112, 185, 339 |
| TaaI | ACNGT | 1 cut(s) | 141 |
| TscAI | CASTG | 2 cut(s) | 299, 325 |
| TseFI | GTSAC | 2 cut(s) | 213, 320 |
| Tsp45I | GTSAC | 2 cut(s) | 213, 320 |
| TspRI | CASTG | 2 cut(s) | 299, 325 |
| VpaK11BI | GGWCC | 2 cut(s) | 116, 169 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 305 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 193 |
| XspI | CTAG | 2 cut(s) | 249, 287 |