ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1145 |
| AccB1I | GGYRCC | 1 cut(s) | 1145 |
| AciI | CCGC | 4 cut(s) | 721, 775, 1067, 1271 |
| AclWI | GGATC | 2 cut(s) | 300, 1111 |
| AcoI | YGGCCR | 2 cut(s) | 1275, 1440 |
| AcsI | RAATTY | 1 cut(s) | 128 |
| AcuI | CTGAAG | 1 cut(s) | 1307 |
| AdeI | CACNNNGTG | 1 cut(s) | 986 |
| AfaI | GTAC | 3 cut(s) | 508, 767, 1147 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 503, 675, 1210, 1322 |
| AflIII | ACRYGT | 1 cut(s) | 1397 |
| AgeI | ACCGGT | 1 cut(s) | 513 |
| AgsI | TTSAA | 1 cut(s) | 184 |
| AjnI | CCWGG | 2 cut(s) | 349, 1048 |
| AluBI | AGCT | 8 cut(s) | 8, 56, 307, 555, 879, 902, 1046, 1432 |
| AluI | AGCT | 8 cut(s) | 8, 56, 307, 555, 879, 902, 1046, 1432 |
| Alw26I | GTCTC | 2 cut(s) | 954, 1219 |
| AlwI | GGATC | 2 cut(s) | 300, 1111 |
| Ama87I | CYCGRG | 1 cut(s) | 1336 |
| AoxI | GGCC | 2 cut(s) | 1275, 1440 |
| ApeKI | GCWGC | 3 cut(s) | 493, 902, 977 |
| ApoI | RAATTY | 1 cut(s) | 128 |
| AsiGI | ACCGGT | 1 cut(s) | 513 |
| Asp718I | GGTACC | 1 cut(s) | 1145 |
| AspS9I | GGNCC | 2 cut(s) | 326, 347 |
| AsuC2I | CCSGG | 2 cut(s) | 26, 486 |
| AsuHPI | GGTGA | 5 cut(s) | 503, 761, 764, 886, 1415 |
| AvaI | CYCGRG | 1 cut(s) | 1336 |
| AvaII | GGWCC | 2 cut(s) | 326, 347 |
| BalI | TGGCCA | 2 cut(s) | 1277, 1442 |
| BanI | GGYRCC | 1 cut(s) | 1145 |
| BbvI | GCAGC | 3 cut(s) | 505, 889, 989 |
| BccI | CCATC | 2 cut(s) | 740, 1052 |
| BciT130I | CCWGG | 2 cut(s) | 351, 1050 |
| BciVI | GTATCC | 1 cut(s) | 1044 |
| BclI | TGATCA | 1 cut(s) | 940 |
| BcnI | CCSGG | 2 cut(s) | 26, 486 |
| BcoDI | GTCTC | 2 cut(s) | 954, 1219 |
| BfaI | CTAG | 1 cut(s) | 417 |
| BfmI | CTRYAG | 1 cut(s) | 1107 |
| BfuI | GTATCC | 1 cut(s) | 1044 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 490 |
| BisI | GCNGC | 4 cut(s) | 494, 903, 978, 1067 |
| BlsI | GCNGC | 4 cut(s) | 495, 904, 979, 1068 |
| BmcAI | AGTACT | 1 cut(s) | 767 |
| Bme1390I | CCNGG | 4 cut(s) | 26, 351, 486, 1050 |
| Bme18I | GGWCC | 2 cut(s) | 326, 347 |
| BmeT110I | CYCGRG | 1 cut(s) | 1336 |
| BmgT120I | GGNCC | 2 cut(s) | 326, 347 |
| BmiI | GGNNCC | 3 cut(s) | 348, 428, 1147 |
| BmrFI | CCNGG | 4 cut(s) | 26, 351, 486, 1050 |
| BmsI | GCATC | 4 cut(s) | 150, 196, 268, 1006 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 628, 660 |
| BpmI | CTGGAG | 2 cut(s) | 1022, 1032 |
| BpuEI | CTTGAG | 3 cut(s) | 230, 486, 725 |
| BpuMI | CCSGG | 2 cut(s) | 26, 486 |
| BsaAI | YACGTR | 1 cut(s) | 311 |
| BsaI | GGTCTC | 1 cut(s) | 1219 |
| BsaJI | CCNNGG | 4 cut(s) | 25, 111, 1126, 1443 |
| BsaWI | WCCGGW | 1 cut(s) | 513 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1030, 1060 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 503, 675, 1210, 1322 |
| Bse118I | RCCGGY | 1 cut(s) | 513 |
| Bse1I | ACTGG | 4 cut(s) | 250, 716, 1005, 1147 |
| Bse3DI | GCAATG | 1 cut(s) | 1249 |
| BseBI | CCWGG | 2 cut(s) | 351, 1050 |
| BseDI | CCNNGG | 4 cut(s) | 25, 111, 1126, 1443 |
| BseGI | GGATG | 6 cut(s) | 165, 259, 751, 871, 1021, 1063 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 503, 675, 1210, 1322 |
| BseMI | GCAATG | 1 cut(s) | 1249 |
| BseMII | CTCAG | 2 cut(s) | 1214, 1223 |
| BseNI | ACTGG | 4 cut(s) | 250, 716, 1005, 1147 |
| BseRI | GAGGAG | 1 cut(s) | 713 |
| BseXI | GCAGC | 3 cut(s) | 505, 889, 989 |
| BshFI | GGCC | 2 cut(s) | 1277, 1442 |
| BshNI | GGYRCC | 1 cut(s) | 1145 |
| BshTI | ACCGGT | 1 cut(s) | 513 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1336 |
| BsiSI | CCGG | 4 cut(s) | 25, 410, 485, 514 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 503, 675, 1210, 1322 |
| BsmAI | GTCTC | 2 cut(s) | 954, 1219 |
| BsmI | GAATGC | 2 cut(s) | 360, 979 |
| BsnI | GGCC | 2 cut(s) | 1277, 1442 |
| Bso31I | GGTCTC | 1 cut(s) | 1219 |
| BsoBI | CYCGRG | 1 cut(s) | 1336 |
| Bsp143I | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| BspACI | CCGC | 4 cut(s) | 721, 775, 1067, 1271 |
| BspANI | GGCC | 2 cut(s) | 1277, 1442 |
| BspCNI | CTCAG | 2 cut(s) | 1215, 1222 |
| BspLI | GGNNCC | 3 cut(s) | 348, 428, 1147 |
| BspPI | GGATC | 2 cut(s) | 300, 1111 |
| BspT107I | GGYRCC | 1 cut(s) | 1145 |
| BspTNI | GGTCTC | 1 cut(s) | 1219 |
| BsrDI | GCAATG | 1 cut(s) | 1249 |
| BsrFI | RCCGGY | 1 cut(s) | 513 |
| BsrI | ACTGG | 4 cut(s) | 250, 716, 1005, 1147 |
| BssAI | RCCGGY | 1 cut(s) | 513 |
| BssECI | CCNNGG | 4 cut(s) | 25, 111, 1126, 1443 |
| BssMI | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| BssT1I | CCWWGG | 3 cut(s) | 111, 1126, 1443 |
| Bst2UI | CCWGG | 2 cut(s) | 351, 1050 |
| Bst4CI | ACNGT | 4 cut(s) | 49, 87, 221, 1283 |
| Bst6I | CTCTTC | 2 cut(s) | 1028, 1079 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 986 |
| BstBAI | YACGTR | 1 cut(s) | 311 |
| BstC8I | GCNNGC | 3 cut(s) | 54, 498, 1064 |
| BstDEI | CTNAG | 3 cut(s) | 762, 1209, 1223 |
| BstEII | GGTNACC | 1 cut(s) | 596 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 501, 673 |
| BstF5I | GGATG | 6 cut(s) | 165, 259, 751, 871, 1021, 1063 |
| BstKTI | GATC | 8 cut(s) | 295, 316, 373, 627, 943, 1106, 1222, 1293 |
| BstMAI | GTCTC | 2 cut(s) | 954, 1219 |
| BstMBI | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 238, 490, 986 |
| BstNI | CCWGG | 2 cut(s) | 351, 1050 |
| BstNSI | RCATGY | 2 cut(s) | 1066, 1401 |
| BstPI | GGTNACC | 1 cut(s) | 596 |
| BstSCI | CCNGG | 4 cut(s) | 24, 349, 484, 1048 |
| BstSFI | CTRYAG | 1 cut(s) | 1107 |
| BstV1I | GCAGC | 3 cut(s) | 505, 889, 989 |
| BstX2I | RGATCY | 2 cut(s) | 292, 1103 |
| BstYI | RGATCY | 2 cut(s) | 292, 1103 |
| BsuI | GTATCC | 1 cut(s) | 1044 |
| BsuRI | GGCC | 2 cut(s) | 1277, 1442 |
| BtsCI | GGATG | 6 cut(s) | 165, 259, 751, 871, 1021, 1063 |
| BtsI | GCAGTG | 1 cut(s) | 183 |
| BtsIMutI | CAGTG | 2 cut(s) | 183, 998 |
| Cac8I | GCNNGC | 3 cut(s) | 54, 498, 1064 |
| Cfr10I | RCCGGY | 1 cut(s) | 513 |
| Cfr13I | GGNCC | 2 cut(s) | 326, 347 |
| Csp6I | GTAC | 3 cut(s) | 507, 766, 1146 |
| CspAI | ACCGGT | 1 cut(s) | 513 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1254, 1289 |
| CviAII | CATG | 6 cut(s) | 15, 119, 443, 452, 1063, 1398 |
| CviQI | GTAC | 3 cut(s) | 507, 766, 1146 |
| DdeI | CTNAG | 3 cut(s) | 762, 1209, 1223 |
| DpnI | GATC | 8 cut(s) | 294, 315, 372, 626, 942, 1105, 1221, 1292 |
| DpnII | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| DraI | TTTAAA | 1 cut(s) | 1428 |
| DraIII | CACNNNGTG | 1 cut(s) | 986 |
| EaeI | YGGCCR | 2 cut(s) | 1275, 1440 |
| Eam1104I | CTCTTC | 2 cut(s) | 1028, 1079 |
| EarI | CTCTTC | 2 cut(s) | 1028, 1079 |
| Eco130I | CCWWGG | 3 cut(s) | 111, 1126, 1443 |
| Eco31I | GGTCTC | 1 cut(s) | 1219 |
| Eco32I | GATATC | 1 cut(s) | 1311 |
| Eco47I | GGWCC | 2 cut(s) | 326, 347 |
| Eco57I | CTGAAG | 1 cut(s) | 1307 |
| Eco88I | CYCGRG | 1 cut(s) | 1336 |
| Eco91I | GGTNACC | 1 cut(s) | 596 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 501, 673 |
| EcoO109I | RGGNCCY | 1 cut(s) | 347 |
| EcoO65I | GGTNACC | 1 cut(s) | 596 |
| EcoRII | CCWGG | 2 cut(s) | 349, 1048 |
| EcoRV | GATATC | 1 cut(s) | 1311 |
| EcoT14I | CCWWGG | 3 cut(s) | 111, 1126, 1443 |
| ErhI | CCWWGG | 3 cut(s) | 111, 1126, 1443 |
| FaeI | CATG | 6 cut(s) | 18, 122, 446, 455, 1066, 1401 |
| FatI | CATG | 6 cut(s) | 14, 118, 442, 451, 1062, 1397 |
| FauI | CCCGC | 1 cut(s) | 714 |
| FauNDI | CATATG | 2 cut(s) | 891, 924 |
| FbaI | TGATCA | 1 cut(s) | 940 |
| Fnu4HI | GCNGC | 4 cut(s) | 494, 903, 978, 1067 |
| FokI | GGATG | 6 cut(s) | 172, 246, 758, 878, 1028, 1070 |
| Fsp4HI | GCNGC | 4 cut(s) | 494, 903, 978, 1067 |
| FspBI | CTAG | 1 cut(s) | 417 |
| GluI | GCNGC | 4 cut(s) | 494, 903, 978, 1067 |
| GsuI | CTGGAG | 2 cut(s) | 1022, 1032 |
| HaeIII | GGCC | 2 cut(s) | 1277, 1442 |
| HapII | CCGG | 4 cut(s) | 25, 410, 485, 514 |
| Hin1II | CATG | 6 cut(s) | 18, 122, 446, 455, 1066, 1401 |
| HincII | GTYRAC | 1 cut(s) | 1165 |
| HindII | GTYRAC | 1 cut(s) | 1165 |
| HindIII | AAGCTT | 1 cut(s) | 1430 |
| HpaI | GTTAAC | 1 cut(s) | 1165 |
| HpaII | CCGG | 4 cut(s) | 25, 410, 485, 514 |
| HphI | GGTGA | 5 cut(s) | 503, 761, 764, 886, 1415 |
| Hpy166II | GTNNAC | 4 cut(s) | 275, 336, 619, 1165 |
| Hpy188I | TCNGA | 9 cut(s) | 438, 543, 850, 954, 1057, 1219, 1224, 1287, 1411 |
| Hpy188III | TCNNGA | 6 cut(s) | 209, 368, 475, 1013, 1027, 1336 |
| Hpy8I | GTNNAC | 4 cut(s) | 275, 336, 619, 1165 |
| Hpy99I | CGWCG | 1 cut(s) | 91 |
| HpyAV | CCTTC | 2 cut(s) | 337, 483 |
| HpyCH4III | ACNGT | 4 cut(s) | 49, 87, 221, 1283 |
| HpyCH4IV | ACGT | 1 cut(s) | 310 |
| HpyCH4V | TGCA | 7 cut(s) | 52, 118, 977, 1019, 1138, 1242, 1328 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 238, 490, 986 |
| HpyF3I | CTNAG | 3 cut(s) | 762, 1209, 1223 |
| HpySE526I | ACGT | 1 cut(s) | 310 |
| Hsp92II | CATG | 6 cut(s) | 18, 122, 446, 455, 1066, 1401 |
| KpnI | GGTACC | 1 cut(s) | 1149 |
| Ksp22I | TGATCA | 1 cut(s) | 940 |
| KspAI | GTTAAC | 1 cut(s) | 1165 |
| Kzo9I | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| LmnI | GCTCC | 2 cut(s) | 304, 1051 |
| Lsp1109I | GCAGC | 3 cut(s) | 505, 889, 989 |
| LweI | GCATC | 4 cut(s) | 150, 196, 268, 1006 |
| MaeI | CTAG | 1 cut(s) | 417 |
| MaeII | ACGT | 1 cut(s) | 310 |
| MaeIII | GTNAC | 4 cut(s) | 265, 596, 664, 958 |
| MalI | GATC | 8 cut(s) | 294, 315, 372, 626, 942, 1105, 1221, 1292 |
| MboI | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| MboII | GAAGA | 6 cut(s) | 134, 1015, 1018, 1066, 1069, 1300 |
| MflI | RGATCY | 2 cut(s) | 292, 1103 |
| MlsI | TGGCCA | 2 cut(s) | 1277, 1442 |
| MluCI | AATT | 5 cut(s) | 19, 128, 152, 166, 1347 |
| MluNI | TGGCCA | 2 cut(s) | 1277, 1442 |
| MlyI | GAGTC | 3 cut(s) | 480, 1201, 1348 |
| MmeI | TCCRAC | 3 cut(s) | 215, 1081, 1105 |
| Mox20I | TGGCCA | 2 cut(s) | 1277, 1442 |
| MscI | TGGCCA | 2 cut(s) | 1277, 1442 |
| MseI | TTAA | 8 cut(s) | 165, 525, 567, 648, 692, 1154, 1164, 1427 |
| MslI | CAYNNNNRTG | 4 cut(s) | 123, 195, 890, 1398 |
| Msp20I | TGGCCA | 2 cut(s) | 1277, 1442 |
| MspA1I | CMGCKG | 1 cut(s) | 721 |
| MspI | CCGG | 4 cut(s) | 25, 410, 485, 514 |
| MspR9I | CCNGG | 4 cut(s) | 26, 351, 486, 1050 |
| Mva1269I | GAATGC | 2 cut(s) | 360, 979 |
| MvaI | CCWGG | 2 cut(s) | 351, 1050 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 238, 490, 986 |
| NciI | CCSGG | 2 cut(s) | 26, 486 |
| NdeI | CATATG | 2 cut(s) | 891, 924 |
| NdeII | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| NlaIII | CATG | 6 cut(s) | 18, 122, 446, 455, 1066, 1401 |
| NlaIV | GGNNCC | 3 cut(s) | 348, 428, 1147 |
| NspI | RCATGY | 2 cut(s) | 1066, 1401 |
| PaeI | GCATGC | 1 cut(s) | 1066 |
| PciI | ACATGT | 1 cut(s) | 1397 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 370 |
| PctI | GAATGC | 2 cut(s) | 360, 979 |
| PfeI | GAWTC | 9 cut(s) | 30, 41, 205, 439, 683, 845, 1009, 1186, 1406 |
| PfoI | TCCNGGA | 1 cut(s) | 349 |
| PinAI | ACCGGT | 1 cut(s) | 513 |
| PkrI | GCNGC | 4 cut(s) | 495, 904, 979, 1068 |
| PleI | GAGTC | 3 cut(s) | 479, 1201, 1347 |
| PpsI | GAGTC | 3 cut(s) | 479, 1201, 1347 |
| Ppu21I | YACGTR | 1 cut(s) | 311 |
| PpuMI | RGGWCCY | 1 cut(s) | 347 |
| PscI | ACATGT | 1 cut(s) | 1397 |
| Psp5II | RGGWCCY | 1 cut(s) | 347 |
| Psp6I | CCWGG | 2 cut(s) | 349, 1048 |
| PspEI | GGTNACC | 1 cut(s) | 596 |
| PspGI | CCWGG | 2 cut(s) | 349, 1048 |
| PspN4I | GGNNCC | 3 cut(s) | 348, 428, 1147 |
| PspPI | GGNCC | 2 cut(s) | 326, 347 |
| PspPPI | RGGWCCY | 1 cut(s) | 347 |
| PsuI | RGATCY | 2 cut(s) | 292, 1103 |
| RsaI | GTAC | 3 cut(s) | 508, 767, 1147 |
| RsaNI | GTAC | 3 cut(s) | 507, 766, 1146 |
| RseI | CAYNNNNRTG | 4 cut(s) | 123, 195, 890, 1398 |
| SaqAI | TTAA | 8 cut(s) | 165, 525, 567, 648, 692, 1154, 1164, 1427 |
| SatI | GCNGC | 4 cut(s) | 494, 903, 978, 1067 |
| Sau3AI | GATC | 8 cut(s) | 292, 313, 370, 624, 940, 1103, 1219, 1290 |
| Sau96I | GGNCC | 2 cut(s) | 326, 347 |
| ScaI | AGTACT | 1 cut(s) | 767 |
| SchI | GAGTC | 3 cut(s) | 480, 1201, 1348 |
| ScrFI | CCNGG | 4 cut(s) | 26, 351, 486, 1050 |
| SfaNI | GCATC | 4 cut(s) | 150, 196, 268, 1006 |
| SfcI | CTRYAG | 1 cut(s) | 1107 |
| SinI | GGWCC | 2 cut(s) | 326, 347 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 123, 195, 890, 1398 |
| SmlI | CTYRAG | 3 cut(s) | 209, 501, 740 |
| SmoI | CTYRAG | 3 cut(s) | 209, 501, 740 |
| SphI | GCATGC | 1 cut(s) | 1066 |
| Sse9I | AATT | 5 cut(s) | 19, 128, 152, 166, 1347 |
| SsiI | CCGC | 4 cut(s) | 721, 775, 1067, 1271 |
| SspMI | CTAG | 1 cut(s) | 417 |
| StyD4I | CCNGG | 4 cut(s) | 24, 349, 484, 1048 |
| StyI | CCWWGG | 3 cut(s) | 111, 1126, 1443 |
| TaaI | ACNGT | 4 cut(s) | 49, 87, 221, 1283 |
| TaiI | ACGT | 1 cut(s) | 313 |
| TaqI | TCGA | 4 cut(s) | 89, 145, 551, 681 |
| TasI | AATT | 5 cut(s) | 19, 128, 152, 166, 1347 |
| TatI | WGTACW | 1 cut(s) | 765 |
| TauI | GCSGC | 1 cut(s) | 1069 |
| TfiI | GAWTC | 9 cut(s) | 30, 41, 205, 439, 683, 845, 1009, 1186, 1406 |
| Tru1I | TTAA | 8 cut(s) | 165, 525, 567, 648, 692, 1154, 1164, 1427 |
| Tru9I | TTAA | 8 cut(s) | 165, 525, 567, 648, 692, 1154, 1164, 1427 |
| TscAI | CASTG | 2 cut(s) | 183, 1005 |
| TseI | GCWGC | 3 cut(s) | 493, 902, 977 |
| TspDTI | ATGAA | 5 cut(s) | 431, 981, 986, 1382, 1428 |
| TspGWI | ACGGA | 1 cut(s) | 1367 |
| TspRI | CASTG | 2 cut(s) | 183, 1005 |
| VpaK11BI | GGWCC | 2 cut(s) | 326, 347 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 501, 673 |
| XapI | RAATTY | 1 cut(s) | 128 |
| XceI | RCATGY | 2 cut(s) | 1066, 1401 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 336 |
| XspI | CTAG | 1 cut(s) | 417 |
| ZrmI | AGTACT | 1 cut(s) | 767 |