ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 188 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 474 |
| Acc36I | ACCTGC | 1 cut(s) | 188 |
| AccB1I | GGYRCC | 1 cut(s) | 807 |
| AccII | CGCG | 1 cut(s) | 466 |
| AciI | CCGC | 7 cut(s) | 304, 342, 464, 602, 965, 988, 1331 |
| AclWI | GGATC | 2 cut(s) | 229, 235 |
| AcsI | RAATTY | 1 cut(s) | 429 |
| AcuI | CTGAAG | 2 cut(s) | 1187, 1203 |
| AdeI | CACNNNGTG | 1 cut(s) | 53 |
| AfaI | GTAC | 2 cut(s) | 1145, 1298 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 92, 348, 631, 803 |
| AgsI | TTSAA | 8 cut(s) | 61, 107, 220, 255, 737, 839, 949, 1292 |
| AjnI | CCWGG | 5 cut(s) | 334, 525, 863, 951, 1312 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 563, 595 |
| AluBI | AGCT | 5 cut(s) | 250, 618, 931, 939, 1037 |
| AluI | AGCT | 5 cut(s) | 250, 618, 931, 939, 1037 |
| Alw26I | GTCTC | 3 cut(s) | 114, 560, 1048 |
| AlwI | GGATC | 2 cut(s) | 229, 235 |
| Ama87I | CYCGRG | 1 cut(s) | 630 |
| AoxI | GGCC | 2 cut(s) | 339, 822 |
| ApeKI | GCWGC | 2 cut(s) | 247, 781 |
| ApoI | RAATTY | 1 cut(s) | 429 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1200 |
| AspA2I | CCTAGG | 1 cut(s) | 154 |
| AspLEI | GCGC | 1 cut(s) | 312 |
| AspS9I | GGNCC | 5 cut(s) | 339, 523, 661, 985, 1316 |
| AsuC2I | CCSGG | 1 cut(s) | 494 |
| AsuHPI | GGTGA | 3 cut(s) | 241, 896, 1285 |
| AvaI | CYCGRG | 1 cut(s) | 630 |
| AvaII | GGWCC | 4 cut(s) | 523, 661, 985, 1316 |
| AvrII | CCTAGG | 1 cut(s) | 154 |
| BaeGI | GKGCMC | 1 cut(s) | 810 |
| BanI | GGYRCC | 1 cut(s) | 807 |
| BbsI | GAAGAC | 2 cut(s) | 389, 466 |
| BbvI | GCAGC | 2 cut(s) | 259, 793 |
| BccI | CCATC | 4 cut(s) | 287, 449, 1172, 1316 |
| BceAI | ACGGC | 4 cut(s) | 65, 615, 935, 1318 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 129, 163 |
| BciT130I | CCWGG | 5 cut(s) | 336, 527, 865, 953, 1314 |
| BclI | TGATCA | 1 cut(s) | 163 |
| BcnI | CCSGG | 1 cut(s) | 494 |
| BcoDI | GTCTC | 3 cut(s) | 114, 560, 1048 |
| BfaI | CTAG | 4 cut(s) | 155, 206, 651, 923 |
| BfoI | RGCGCY | 1 cut(s) | 313 |
| BfuAI | ACCTGC | 1 cut(s) | 188 |
| BglII | AGATCT | 1 cut(s) | 63 |
| BisI | GCNGC | 4 cut(s) | 248, 342, 782, 1331 |
| BlnI | CCTAGG | 1 cut(s) | 154 |
| BlsI | GCNGC | 4 cut(s) | 249, 343, 783, 1332 |
| Bme1390I | CCNGG | 6 cut(s) | 336, 494, 527, 865, 953, 1314 |
| Bme18I | GGWCC | 4 cut(s) | 523, 661, 985, 1316 |
| BmeT110I | CYCGRG | 1 cut(s) | 630 |
| BmgT120I | GGNCC | 5 cut(s) | 339, 523, 661, 985, 1316 |
| BmiI | GGNNCC | 4 cut(s) | 98, 340, 491, 809 |
| BmrFI | CCNGG | 6 cut(s) | 336, 494, 527, 865, 953, 1314 |
| BmrI | ACTGGG | 1 cut(s) | 1249 |
| BmsI | GCATC | 3 cut(s) | 137, 262, 850 |
| BmuI | ACTGGG | 1 cut(s) | 1249 |
| BpiI | GAAGAC | 2 cut(s) | 389, 466 |
| BpuEI | CTTGAG | 2 cut(s) | 356, 1037 |
| BpuMI | CCSGG | 1 cut(s) | 494 |
| BsaBI | GATNNNNATC | 2 cut(s) | 68, 239 |
| BsaJI | CCNNGG | 4 cut(s) | 154, 335, 401, 526 |
| BsaWI | WCCGGW | 3 cut(s) | 469, 796, 1318 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1004, 1034, 1271, 1301 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 92, 348, 631, 803 |
| Bse1I | ACTGG | 3 cut(s) | 850, 861, 1255 |
| Bse3DI | GCAATG | 1 cut(s) | 282 |
| Bse8I | GATNNNNATC | 2 cut(s) | 68, 239 |
| BseBI | CCWGG | 5 cut(s) | 336, 527, 865, 953, 1314 |
| BseDI | CCNNGG | 4 cut(s) | 154, 335, 401, 526 |
| BseGI | GGATG | 5 cut(s) | 460, 621, 636, 865, 1327 |
| BseJI | GATNNNNATC | 2 cut(s) | 68, 239 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 92, 348, 631, 803 |
| BseMI | GCAATG | 1 cut(s) | 282 |
| BseNI | ACTGG | 3 cut(s) | 850, 861, 1255 |
| BseRI | GAGGAG | 5 cut(s) | 941, 985, 1025, 1244, 1283 |
| BseSI | GKGCMC | 1 cut(s) | 810 |
| BseXI | GCAGC | 2 cut(s) | 259, 793 |
| BsgI | GTGCAG | 1 cut(s) | 711 |
| Bsh1236I | CGCG | 1 cut(s) | 466 |
| Bsh1285I | CGRYCG | 1 cut(s) | 470 |
| BshFI | GGCC | 2 cut(s) | 341, 824 |
| BshNI | GGYRCC | 1 cut(s) | 807 |
| BsiEI | CGRYCG | 1 cut(s) | 470 |
| BsiHKCI | CYCGRG | 1 cut(s) | 630 |
| BsiSI | CCGG | 5 cut(s) | 244, 470, 493, 797, 1319 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 92, 348, 631, 803 |
| BsmAI | GTCTC | 3 cut(s) | 114, 560, 1048 |
| BsmBI | CGTCTC | 2 cut(s) | 114, 560 |
| BsmI | GAATGC | 1 cut(s) | 1332 |
| BsnI | GGCC | 2 cut(s) | 341, 824 |
| BsoBI | CYCGRG | 1 cut(s) | 630 |
| Bsp1286I | GDGCHC | 1 cut(s) | 810 |
| Bsp143I | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| BspACI | CCGC | 7 cut(s) | 304, 342, 464, 602, 965, 988, 1331 |
| BspANI | GGCC | 2 cut(s) | 341, 824 |
| BspFNI | CGCG | 1 cut(s) | 466 |
| BspHI | TCATGA | 1 cut(s) | 766 |
| BspLI | GGNNCC | 4 cut(s) | 98, 340, 491, 809 |
| BspMI | ACCTGC | 1 cut(s) | 188 |
| BspPI | GGATC | 2 cut(s) | 229, 235 |
| BspT107I | GGYRCC | 1 cut(s) | 807 |
| BsrDI | GCAATG | 1 cut(s) | 282 |
| BsrI | ACTGG | 3 cut(s) | 850, 861, 1255 |
| BssECI | CCNNGG | 4 cut(s) | 154, 335, 401, 526 |
| BssMI | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| BssT1I | CCWWGG | 1 cut(s) | 154 |
| Bst2UI | CCWGG | 5 cut(s) | 336, 527, 865, 953, 1314 |
| Bst4CI | ACNGT | 4 cut(s) | 329, 395, 753, 1132 |
| Bst6I | CTCTTC | 2 cut(s) | 219, 1206 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 860 |
| BstC8I | GCNNGC | 3 cut(s) | 54, 195, 1035 |
| BstDEI | CTNAG | 2 cut(s) | 111, 935 |
| BstDSI | CCRYGG | 1 cut(s) | 401 |
| BstF5I | GGATG | 5 cut(s) | 460, 621, 636, 865, 1327 |
| BstFNI | CGCG | 1 cut(s) | 466 |
| BstH2I | RGCGCY | 1 cut(s) | 313 |
| BstHHI | GCGC | 1 cut(s) | 312 |
| BstKTI | GATC | 9 cut(s) | 66, 72, 117, 166, 237, 243, 903, 917, 1066 |
| BstMAI | GTCTC | 3 cut(s) | 114, 560, 1048 |
| BstMBI | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| BstMCI | CGRYCG | 1 cut(s) | 470 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 787, 860, 928, 962 |
| BstNI | CCWGG | 5 cut(s) | 336, 527, 865, 953, 1314 |
| BstSCI | CCNGG | 6 cut(s) | 334, 492, 525, 863, 951, 1312 |
| BstSLI | GKGCMC | 1 cut(s) | 810 |
| BstUI | CGCG | 1 cut(s) | 466 |
| BstV1I | GCAGC | 2 cut(s) | 259, 793 |
| BstV2I | GAAGAC | 2 cut(s) | 389, 466 |
| BstX2I | RGATCY | 3 cut(s) | 63, 234, 240 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 552 |
| BstYI | RGATCY | 3 cut(s) | 63, 234, 240 |
| BsuRI | GGCC | 2 cut(s) | 341, 824 |
| BtgI | CCRYGG | 1 cut(s) | 401 |
| BtgZI | GCGATG | 1 cut(s) | 160 |
| BtsCI | GGATG | 5 cut(s) | 460, 621, 636, 865, 1327 |
| BtsI | GCAGTG | 1 cut(s) | 791 |
| BtsIMutI | CAGTG | 3 cut(s) | 791, 854, 1137 |
| BveI | ACCTGC | 1 cut(s) | 188 |
| Cac8I | GCNNGC | 3 cut(s) | 54, 195, 1035 |
| CciI | TCATGA | 1 cut(s) | 766 |
| CfoI | GCGC | 1 cut(s) | 312 |
| Cfr13I | GGNCC | 5 cut(s) | 339, 523, 661, 985, 1316 |
| CseI | GACGC | 1 cut(s) | 131 |
| Csp6I | GTAC | 2 cut(s) | 1144, 1297 |
| CviAII | CATG | 4 cut(s) | 167, 767, 1047, 1072 |
| CviQI | GTAC | 2 cut(s) | 1144, 1297 |
| DdeI | CTNAG | 2 cut(s) | 111, 935 |
| DpnI | GATC | 9 cut(s) | 65, 71, 116, 165, 236, 242, 902, 916, 1065 |
| DpnII | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| DraIII | CACNNNGTG | 1 cut(s) | 53 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 474 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 474 |
| Eam1104I | CTCTTC | 2 cut(s) | 219, 1206 |
| EarI | CTCTTC | 2 cut(s) | 219, 1206 |
| EciI | GGCGGA | 2 cut(s) | 617, 980 |
| Eco130I | CCWWGG | 1 cut(s) | 154 |
| Eco32I | GATATC | 1 cut(s) | 719 |
| Eco47I | GGWCC | 4 cut(s) | 523, 661, 985, 1316 |
| Eco57I | CTGAAG | 2 cut(s) | 1187, 1203 |
| Eco88I | CYCGRG | 1 cut(s) | 630 |
| EcoRII | CCWGG | 5 cut(s) | 334, 525, 863, 951, 1312 |
| EcoRV | GATATC | 1 cut(s) | 719 |
| EcoT14I | CCWWGG | 1 cut(s) | 154 |
| ErhI | CCWWGG | 1 cut(s) | 154 |
| Esp3I | CGTCTC | 2 cut(s) | 114, 560 |
| FaeI | CATG | 4 cut(s) | 170, 770, 1050, 1075 |
| FatI | CATG | 4 cut(s) | 166, 766, 1046, 1071 |
| FbaI | TGATCA | 1 cut(s) | 163 |
| Fnu4HI | GCNGC | 4 cut(s) | 248, 342, 782, 1331 |
| FokI | GGATG | 5 cut(s) | 467, 608, 623, 872, 1334 |
| Fsp4HI | GCNGC | 4 cut(s) | 248, 342, 782, 1331 |
| FspBI | CTAG | 4 cut(s) | 155, 206, 651, 923 |
| GlaI | GCGC | 1 cut(s) | 311 |
| GluI | GCNGC | 4 cut(s) | 248, 342, 782, 1331 |
| HaeII | RGCGCY | 1 cut(s) | 313 |
| HaeIII | GGCC | 2 cut(s) | 341, 824 |
| HapII | CCGG | 5 cut(s) | 244, 470, 493, 797, 1319 |
| HgaI | GACGC | 1 cut(s) | 131 |
| HhaI | GCGC | 1 cut(s) | 312 |
| Hin1II | CATG | 4 cut(s) | 170, 770, 1050, 1075 |
| Hin6I | GCGC | 1 cut(s) | 310 |
| HinP1I | GCGC | 1 cut(s) | 310 |
| HinfI | GANTC | 7 cut(s) | 259, 356, 475, 560, 737, 910, 1027 |
| HpaII | CCGG | 5 cut(s) | 244, 470, 493, 797, 1319 |
| HphI | GGTGA | 3 cut(s) | 241, 896, 1285 |
| Hpy166II | GTNNAC | 1 cut(s) | 523 |
| Hpy188I | TCNGA | 6 cut(s) | 119, 565, 613, 661, 672, 1009 |
| Hpy188III | TCNNGA | 7 cut(s) | 16, 67, 100, 217, 238, 706, 767 |
| Hpy8I | GTNNAC | 1 cut(s) | 523 |
| HpyAV | CCTTC | 5 cut(s) | 51, 281, 565, 1227, 1270 |
| HpyCH4III | ACNGT | 4 cut(s) | 329, 395, 753, 1132 |
| HpyCH4IV | ACGT | 2 cut(s) | 6, 793 |
| HpyCH4V | TGCA | 6 cut(s) | 150, 170, 275, 512, 692, 854 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 787, 860, 928, 962 |
| HpyF3I | CTNAG | 2 cut(s) | 111, 935 |
| HpySE526I | ACGT | 2 cut(s) | 6, 793 |
| Hsp92II | CATG | 4 cut(s) | 170, 770, 1050, 1075 |
| HspAI | GCGC | 1 cut(s) | 310 |
| Ksp22I | TGATCA | 1 cut(s) | 163 |
| Kzo9I | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| LmnI | GCTCC | 1 cut(s) | 928 |
| Lsp1109I | GCAGC | 2 cut(s) | 259, 793 |
| LweI | GCATC | 3 cut(s) | 137, 262, 850 |
| MaeI | CTAG | 4 cut(s) | 155, 206, 651, 923 |
| MaeII | ACGT | 2 cut(s) | 6, 793 |
| MaeIII | GTNAC | 2 cut(s) | 747, 1222 |
| MalI | GATC | 9 cut(s) | 65, 71, 116, 165, 236, 242, 902, 916, 1065 |
| MboI | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| MflI | RGATCY | 3 cut(s) | 63, 234, 240 |
| MhlI | GDGCHC | 1 cut(s) | 810 |
| MluCI | AATT | 6 cut(s) | 19, 89, 429, 449, 515, 1016 |
| MlyI | GAGTC | 3 cut(s) | 253, 484, 1036 |
| MmeI | TCCRAC | 2 cut(s) | 561, 639 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1200 |
| MseI | TTAA | 1 cut(s) | 423 |
| MspA1I | CMGCKG | 1 cut(s) | 344 |
| MspI | CCGG | 5 cut(s) | 244, 470, 493, 797, 1319 |
| MspR9I | CCNGG | 6 cut(s) | 336, 494, 527, 865, 953, 1314 |
| Mva1269I | GAATGC | 1 cut(s) | 1332 |
| MvaI | CCWGG | 5 cut(s) | 336, 527, 865, 953, 1314 |
| MvnI | CGCG | 1 cut(s) | 466 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 787, 860, 928, 962 |
| NciI | CCSGG | 1 cut(s) | 494 |
| NdeII | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| NlaIII | CATG | 4 cut(s) | 170, 770, 1050, 1075 |
| NlaIV | GGNNCC | 4 cut(s) | 98, 340, 491, 809 |
| PagI | TCATGA | 1 cut(s) | 766 |
| PaqCI | CACCTGC | 1 cut(s) | 188 |
| PctI | GAATGC | 1 cut(s) | 1332 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1200 |
| PfeI | GAWTC | 4 cut(s) | 356, 560, 737, 910 |
| PfoI | TCCNGGA | 1 cut(s) | 492 |
| PkrI | GCNGC | 4 cut(s) | 249, 343, 783, 1332 |
| PleI | GAGTC | 3 cut(s) | 253, 483, 1035 |
| PpsI | GAGTC | 3 cut(s) | 253, 483, 1035 |
| Psp6I | CCWGG | 5 cut(s) | 334, 525, 863, 951, 1312 |
| PspGI | CCWGG | 5 cut(s) | 334, 525, 863, 951, 1312 |
| PspN4I | GGNNCC | 4 cut(s) | 98, 340, 491, 809 |
| PspPI | GGNCC | 5 cut(s) | 339, 523, 661, 985, 1316 |
| PsuI | RGATCY | 3 cut(s) | 63, 234, 240 |
| RsaI | GTAC | 2 cut(s) | 1145, 1298 |
| RsaNI | GTAC | 2 cut(s) | 1144, 1297 |
| SaqAI | TTAA | 1 cut(s) | 423 |
| SatI | GCNGC | 4 cut(s) | 248, 342, 782, 1331 |
| Sau3AI | GATC | 9 cut(s) | 63, 69, 114, 163, 234, 240, 900, 914, 1063 |
| Sau96I | GGNCC | 5 cut(s) | 339, 523, 661, 985, 1316 |
| SchI | GAGTC | 3 cut(s) | 253, 484, 1036 |
| ScrFI | CCNGG | 6 cut(s) | 336, 494, 527, 865, 953, 1314 |
| SduI | GDGCHC | 1 cut(s) | 810 |
| SfaNI | GCATC | 3 cut(s) | 137, 262, 850 |
| SinI | GGWCC | 4 cut(s) | 523, 661, 985, 1316 |
| SmlI | CTYRAG | 2 cut(s) | 371, 1052 |
| SmoI | CTYRAG | 2 cut(s) | 371, 1052 |
| Sse9I | AATT | 6 cut(s) | 19, 89, 429, 449, 515, 1016 |
| SsiI | CCGC | 7 cut(s) | 304, 342, 464, 602, 965, 988, 1331 |
| SspMI | CTAG | 4 cut(s) | 155, 206, 651, 923 |
| StyD4I | CCNGG | 6 cut(s) | 334, 492, 525, 863, 951, 1312 |
| StyI | CCWWGG | 1 cut(s) | 154 |
| TaaI | ACNGT | 4 cut(s) | 329, 395, 753, 1132 |
| TaiI | ACGT | 2 cut(s) | 9, 796 |
| TaqI | TCGA | 4 cut(s) | 25, 135, 827, 913 |
| TasI | AATT | 6 cut(s) | 19, 89, 429, 449, 515, 1016 |
| TauI | GCSGC | 2 cut(s) | 344, 1333 |
| TfiI | GAWTC | 4 cut(s) | 356, 560, 737, 910 |
| Tru1I | TTAA | 1 cut(s) | 423 |
| Tru9I | TTAA | 1 cut(s) | 423 |
| TscAI | CASTG | 3 cut(s) | 791, 861, 1137 |
| TseI | GCWGC | 2 cut(s) | 247, 781 |
| TspDTI | ATGAA | 5 cut(s) | 17, 237, 610, 1104, 1250 |
| TspGWI | ACGGA | 1 cut(s) | 390 |
| TspRI | CASTG | 3 cut(s) | 791, 861, 1137 |
| VpaK11BI | GGWCC | 4 cut(s) | 523, 661, 985, 1316 |
| XapI | RAATTY | 1 cut(s) | 429 |
| XmaJI | CCTAGG | 1 cut(s) | 154 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1200 |
| XspI | CTAG | 4 cut(s) | 155, 206, 651, 923 |