Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 271 |
| AciI | CCGC | 2 cut(s) | 176, 278 |
| AclWI | GGATC | 2 cut(s) | 465, 603 |
| AcsI | RAATTY | 3 cut(s) | 36, 493, 665 |
| AcuI | CTGAAG | 2 cut(s) | 86, 635 |
| AfaI | GTAC | 3 cut(s) | 532, 545, 625 |
| AfeI | AGCGCT | 1 cut(s) | 159 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 133, 271, 345 |
| AgsI | TTSAA | 1 cut(s) | 664 |
| AluBI | AGCT | 2 cut(s) | 370, 482 |
| AluI | AGCT | 2 cut(s) | 370, 482 |
| Alw21I | GWGCWC | 1 cut(s) | 189 |
| Alw26I | GTCTC | 3 cut(s) | 107, 305, 505 |
| AlwI | GGATC | 2 cut(s) | 465, 603 |
| Aor51HI | AGCGCT | 1 cut(s) | 159 |
| AoxI | GGCC | 2 cut(s) | 89, 672 |
| ApeKI | GCWGC | 1 cut(s) | 367 |
| ApoI | RAATTY | 3 cut(s) | 36, 493, 665 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 569 |
| AspA2I | CCTAGG | 1 cut(s) | 436 |
| AspLEI | GCGC | 1 cut(s) | 160 |
| AspS9I | GGNCC | 2 cut(s) | 70, 448 |
| AsuC2I | CCSGG | 1 cut(s) | 521 |
| AsuII | TTCGAA | 1 cut(s) | 563 |
| AvaII | GGWCC | 2 cut(s) | 70, 448 |
| AvrII | CCTAGG | 1 cut(s) | 436 |
| BaeGI | GKGCMC | 1 cut(s) | 606 |
| BanII | GRGCYC | 1 cut(s) | 64 |
| Bbv12I | GWGCWC | 1 cut(s) | 189 |
| BbvI | GCAGC | 1 cut(s) | 379 |
| BccI | CCATC | 3 cut(s) | 241, 273, 592 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 356, 390 |
| BcnI | CCSGG | 1 cut(s) | 521 |
| BcoDI | GTCTC | 3 cut(s) | 107, 305, 505 |
| BfaI | CTAG | 5 cut(s) | 392, 437, 461, 621, 642 |
| BfmI | CTRYAG | 1 cut(s) | 477 |
| BfoI | RGCGCY | 1 cut(s) | 161 |
| BisI | GCNGC | 1 cut(s) | 368 |
| BlnI | CCTAGG | 1 cut(s) | 436 |
| BlsI | GCNGC | 1 cut(s) | 369 |
| Bme1390I | CCNGG | 1 cut(s) | 521 |
| Bme18I | GGWCC | 2 cut(s) | 70, 448 |
| BmgT120I | GGNCC | 2 cut(s) | 70, 448 |
| BmiI | GGNNCC | 1 cut(s) | 449 |
| BmrFI | CCNGG | 1 cut(s) | 521 |
| BmsI | GCATC | 5 cut(s) | 160, 265, 283, 647, 661 |
| BpmI | CTGGAG | 1 cut(s) | 225 |
| Bpu14I | TTCGAA | 1 cut(s) | 563 |
| BpuMI | CCSGG | 1 cut(s) | 521 |
| BsaBI | GATNNNNATC | 1 cut(s) | 594 |
| BsaJI | CCNNGG | 2 cut(s) | 404, 436 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 133, 271, 345 |
| Bse1I | ACTGG | 2 cut(s) | 297, 325 |
| Bse8I | GATNNNNATC | 1 cut(s) | 594 |
| BseDI | CCNNGG | 2 cut(s) | 404, 436 |
| BseGI | GGATG | 4 cut(s) | 16, 252, 280, 298 |
| BseJI | GATNNNNATC | 1 cut(s) | 594 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 133, 271, 345 |
| BseMII | CTCAG | 3 cut(s) | 107, 202, 233 |
| BseNI | ACTGG | 2 cut(s) | 297, 325 |
| BseRI | GAGGAG | 1 cut(s) | 621 |
| BseSI | GKGCMC | 1 cut(s) | 606 |
| BseXI | GCAGC | 1 cut(s) | 379 |
| BshFI | GGCC | 2 cut(s) | 91, 674 |
| BsiHKAI | GWGCWC | 1 cut(s) | 189 |
| BsiSI | CCGG | 1 cut(s) | 520 |
| BslFI | GGGAC | 1 cut(s) | 461 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 133, 271, 345 |
| BsmAI | GTCTC | 3 cut(s) | 107, 305, 505 |
| BsmFI | GGGAC | 1 cut(s) | 461 |
| BsmI | GAATGC | 2 cut(s) | 178, 559 |
| BsnI | GGCC | 2 cut(s) | 91, 674 |
| Bsp119I | TTCGAA | 1 cut(s) | 563 |
| Bsp1286I | GDGCHC | 3 cut(s) | 64, 189, 606 |
| Bsp143I | GATC | 3 cut(s) | 355, 457, 595 |
| Bsp19I | CCATGG | 1 cut(s) | 404 |
| BspACI | CCGC | 2 cut(s) | 176, 278 |
| BspANI | GGCC | 2 cut(s) | 91, 674 |
| BspCNI | CTCAG | 3 cut(s) | 106, 201, 234 |
| BspLI | GGNNCC | 1 cut(s) | 449 |
| BspPI | GGATC | 2 cut(s) | 465, 603 |
| BspT104I | TTCGAA | 1 cut(s) | 563 |
| BsrI | ACTGG | 2 cut(s) | 297, 325 |
| BssECI | CCNNGG | 2 cut(s) | 404, 436 |
| BssMI | GATC | 3 cut(s) | 355, 457, 595 |
| BssT1I | CCWWGG | 2 cut(s) | 404, 436 |
| BstBI | TTCGAA | 1 cut(s) | 563 |
| BstDEI | CTNAG | 4 cut(s) | 17, 93, 188, 242 |
| BstDSI | CCRYGG | 1 cut(s) | 404 |
| BstF5I | GGATG | 4 cut(s) | 16, 252, 280, 298 |
| BstH2I | RGCGCY | 1 cut(s) | 161 |
| BstHHI | GCGC | 1 cut(s) | 160 |
| BstKTI | GATC | 3 cut(s) | 358, 460, 598 |
| BstMAI | GTCTC | 3 cut(s) | 107, 305, 505 |
| BstMBI | GATC | 3 cut(s) | 355, 457, 595 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 157, 610 |
| BstSCI | CCNGG | 1 cut(s) | 519 |
| BstSFI | CTRYAG | 1 cut(s) | 477 |
| BstSLI | GKGCMC | 1 cut(s) | 606 |
| BstV1I | GCAGC | 1 cut(s) | 379 |
| BstX2I | RGATCY | 1 cut(s) | 457 |
| BstYI | RGATCY | 1 cut(s) | 457 |
| BsuRI | GGCC | 2 cut(s) | 91, 674 |
| BtgI | CCRYGG | 1 cut(s) | 404 |
| BtsCI | GGATG | 4 cut(s) | 16, 252, 280, 298 |
| BtsI | GCAGTG | 1 cut(s) | 531 |
| BtsIMutI | CAGTG | 2 cut(s) | 196, 531 |
| CfoI | GCGC | 1 cut(s) | 160 |
| Cfr13I | GGNCC | 2 cut(s) | 70, 448 |
| Csp6I | GTAC | 3 cut(s) | 531, 544, 624 |
| CviAII | CATG | 5 cut(s) | 7, 405, 470, 486, 599 |
| CviQI | GTAC | 3 cut(s) | 531, 544, 624 |
| DdeI | CTNAG | 4 cut(s) | 17, 93, 188, 242 |
| DpnI | GATC | 3 cut(s) | 357, 459, 597 |
| DpnII | GATC | 3 cut(s) | 355, 457, 595 |
| Eco130I | CCWWGG | 2 cut(s) | 404, 436 |
| Eco147I | AGGCCT | 2 cut(s) | 91, 674 |
| Eco24I | GRGCYC | 1 cut(s) | 64 |
| Eco47I | GGWCC | 2 cut(s) | 70, 448 |
| Eco47III | AGCGCT | 1 cut(s) | 159 |
| Eco57I | CTGAAG | 2 cut(s) | 86, 635 |
| EcoO109I | RGGNCCY | 2 cut(s) | 70, 448 |
| EcoT14I | CCWWGG | 2 cut(s) | 404, 436 |
| EcoT38I | GRGCYC | 1 cut(s) | 64 |
| ErhI | CCWWGG | 2 cut(s) | 404, 436 |
| FaeI | CATG | 5 cut(s) | 10, 408, 473, 489, 602 |
| FaqI | GGGAC | 1 cut(s) | 461 |
| FatI | CATG | 5 cut(s) | 6, 404, 469, 485, 598 |
| FauNDI | CATATG | 1 cut(s) | 270 |
| Fnu4HI | GCNGC | 1 cut(s) | 368 |
| FokI | GGATG | 4 cut(s) | 23, 259, 287, 305 |
| FriOI | GRGCYC | 1 cut(s) | 64 |
| Fsp4HI | GCNGC | 1 cut(s) | 368 |
| FspBI | CTAG | 5 cut(s) | 392, 437, 461, 621, 642 |
| GlaI | GCGC | 1 cut(s) | 159 |
| GluI | GCNGC | 1 cut(s) | 368 |
| GsuI | CTGGAG | 1 cut(s) | 225 |
| HaeII | RGCGCY | 1 cut(s) | 161 |
| HaeIII | GGCC | 2 cut(s) | 91, 674 |
| HapII | CCGG | 1 cut(s) | 520 |
| HhaI | GCGC | 1 cut(s) | 160 |
| Hin1II | CATG | 5 cut(s) | 10, 408, 473, 489, 602 |
| Hin6I | GCGC | 1 cut(s) | 158 |
| HinP1I | GCGC | 1 cut(s) | 158 |
| HinfI | GANTC | 2 cut(s) | 395, 590 |
| HpaII | CCGG | 1 cut(s) | 520 |
| Hpy166II | GTNNAC | 1 cut(s) | 531 |
| Hpy188I | TCNGA | 5 cut(s) | 66, 96, 388, 589, 595 |
| Hpy188III | TCNNGA | 5 cut(s) | 204, 223, 377, 428, 642 |
| Hpy8I | GTNNAC | 1 cut(s) | 531 |
| HpyAV | CCTTC | 6 cut(s) | 61, 123, 315, 383, 610, 685 |
| HpyCH4IV | ACGT | 1 cut(s) | 53 |
| HpyCH4V | TGCA | 2 cut(s) | 30, 151 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 157, 610 |
| HpyF3I | CTNAG | 4 cut(s) | 17, 93, 188, 242 |
| HpySE526I | ACGT | 1 cut(s) | 53 |
| Hsp92II | CATG | 5 cut(s) | 10, 408, 473, 489, 602 |
| HspAI | GCGC | 1 cut(s) | 158 |
| Kzo9I | GATC | 3 cut(s) | 355, 457, 595 |
| LmnI | GCTCC | 1 cut(s) | 375 |
| Lsp1109I | GCAGC | 1 cut(s) | 379 |
| LweI | GCATC | 5 cut(s) | 160, 265, 283, 647, 661 |
| MaeI | CTAG | 5 cut(s) | 392, 437, 461, 621, 642 |
| MaeII | ACGT | 1 cut(s) | 53 |
| MaeIII | GTNAC | 1 cut(s) | 377 |
| MalI | GATC | 3 cut(s) | 357, 459, 597 |
| MboI | GATC | 3 cut(s) | 355, 457, 595 |
| MboII | GAAGA | 1 cut(s) | 528 |
| MflI | RGATCY | 1 cut(s) | 457 |
| MhlI | GDGCHC | 3 cut(s) | 64, 189, 606 |
| MluCI | AATT | 6 cut(s) | 25, 36, 493, 506, 569, 665 |
| MlyI | GAGTC | 1 cut(s) | 389 |
| MmeI | TCCRAC | 1 cut(s) | 435 |
| MroXI | GAANNNNTTC | 1 cut(s) | 569 |
| MseI | TTAA | 1 cut(s) | 317 |
| MslI | CAYNNNNRTG | 2 cut(s) | 11, 273 |
| MspI | CCGG | 1 cut(s) | 520 |
| MspR9I | CCNGG | 1 cut(s) | 521 |
| Mva1269I | GAATGC | 2 cut(s) | 178, 559 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 157, 610 |
| NciI | CCSGG | 1 cut(s) | 521 |
| NcoI | CCATGG | 1 cut(s) | 404 |
| NdeI | CATATG | 1 cut(s) | 270 |
| NdeII | GATC | 3 cut(s) | 355, 457, 595 |
| NlaIII | CATG | 5 cut(s) | 10, 408, 473, 489, 602 |
| NlaIV | GGNNCC | 1 cut(s) | 449 |
| NmuCI | GTSAC | 1 cut(s) | 377 |
| NspV | TTCGAA | 1 cut(s) | 563 |
| PceI | AGGCCT | 2 cut(s) | 91, 674 |
| PctI | GAATGC | 2 cut(s) | 178, 559 |
| PdmI | GAANNNNTTC | 1 cut(s) | 569 |
| PfeI | GAWTC | 1 cut(s) | 590 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 271 |
| PkrI | GCNGC | 1 cut(s) | 369 |
| PleI | GAGTC | 1 cut(s) | 389 |
| PpsI | GAGTC | 1 cut(s) | 389 |
| PpuMI | RGGWCCY | 2 cut(s) | 70, 448 |
| Psp5II | RGGWCCY | 2 cut(s) | 70, 448 |
| PspN4I | GGNNCC | 1 cut(s) | 449 |
| PspPI | GGNCC | 2 cut(s) | 70, 448 |
| PspPPI | RGGWCCY | 2 cut(s) | 70, 448 |
| PsuI | RGATCY | 1 cut(s) | 457 |
| RsaI | GTAC | 3 cut(s) | 532, 545, 625 |
| RsaNI | GTAC | 3 cut(s) | 531, 544, 624 |
| RseI | CAYNNNNRTG | 2 cut(s) | 11, 273 |
| SaqAI | TTAA | 1 cut(s) | 317 |
| SatI | GCNGC | 1 cut(s) | 368 |
| Sau3AI | GATC | 3 cut(s) | 355, 457, 595 |
| Sau96I | GGNCC | 2 cut(s) | 70, 448 |
| SchI | GAGTC | 1 cut(s) | 389 |
| ScrFI | CCNGG | 1 cut(s) | 521 |
| SduI | GDGCHC | 3 cut(s) | 64, 189, 606 |
| SetI | ASST | 9 cut(s) | 56, 75, 237, 372, 384, 393, 442, 453, 484 |
| SfaNI | GCATC | 5 cut(s) | 160, 265, 283, 647, 661 |
| SfcI | CTRYAG | 1 cut(s) | 477 |
| SfuI | TTCGAA | 1 cut(s) | 563 |
| SinI | GGWCC | 2 cut(s) | 70, 448 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 11, 273 |
| Sse9I | AATT | 6 cut(s) | 25, 36, 493, 506, 569, 665 |
| SseBI | AGGCCT | 2 cut(s) | 91, 674 |
| SsiI | CCGC | 2 cut(s) | 176, 278 |
| SspMI | CTAG | 5 cut(s) | 392, 437, 461, 621, 642 |
| StuI | AGGCCT | 2 cut(s) | 91, 674 |
| StyD4I | CCNGG | 1 cut(s) | 519 |
| StyI | CCWWGG | 2 cut(s) | 404, 436 |
| TaiI | ACGT | 1 cut(s) | 56 |
| TaqI | TCGA | 2 cut(s) | 154, 563 |
| TasI | AATT | 6 cut(s) | 25, 36, 493, 506, 569, 665 |
| TatI | WGTACW | 2 cut(s) | 530, 623 |
| TfiI | GAWTC | 1 cut(s) | 590 |
| Tru1I | TTAA | 1 cut(s) | 317 |
| Tru9I | TTAA | 1 cut(s) | 317 |
| TscAI | CASTG | 2 cut(s) | 196, 531 |
| TseFI | GTSAC | 1 cut(s) | 377 |
| TseI | GCWGC | 1 cut(s) | 367 |
| Tsp45I | GTSAC | 1 cut(s) | 377 |
| TspDTI | ATGAA | 2 cut(s) | 60, 458 |
| TspRI | CASTG | 2 cut(s) | 196, 531 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 271 |
| VpaK11BI | GGWCC | 2 cut(s) | 70, 448 |
| XapI | RAATTY | 3 cut(s) | 36, 493, 665 |
| XbaI | TCTAGA | 1 cut(s) | 641 |
| XmaJI | CCTAGG | 1 cut(s) | 436 |
| XmnI | GAANNNNTTC | 1 cut(s) | 569 |
| XspI | CTAG | 5 cut(s) | 392, 437, 461, 621, 642 |