ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 567 |
| AccI | GTMKAC | 1 cut(s) | 301 |
| AciI | CCGC | 6 cut(s) | 47, 176, 342, 571, 600, 626 |
| AclWI | GGATC | 2 cut(s) | 109, 646 |
| AcoI | YGGCCR | 1 cut(s) | 328 |
| AfaI | GTAC | 2 cut(s) | 358, 689 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 151, 448, 606, 656 |
| AgsI | TTSAA | 3 cut(s) | 424, 436, 638 |
| AjiI | CACGTC | 1 cut(s) | 271 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 219, 251 |
| AluBI | AGCT | 1 cut(s) | 430 |
| AluI | AGCT | 1 cut(s) | 430 |
| AlwI | GGATC | 2 cut(s) | 109, 646 |
| AoxI | GGCC | 4 cut(s) | 328, 475, 560, 695 |
| ApeKI | GCWGC | 3 cut(s) | 44, 73, 484 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 219, 251 |
| AspLEI | GCGC | 2 cut(s) | 63, 351 |
| AspS9I | GGNCC | 1 cut(s) | 560 |
| AsuC2I | CCSGG | 1 cut(s) | 564 |
| AsuHPI | GGTGA | 2 cut(s) | 428, 470 |
| AsuII | TTCGAA | 1 cut(s) | 248 |
| BalI | TGGCCA | 1 cut(s) | 330 |
| BauI | CACGAG | 1 cut(s) | 13 |
| BbvI | GCAGC | 3 cut(s) | 56, 85, 471 |
| BccI | CCATC | 3 cut(s) | 284, 300, 339 |
| BceAI | ACGGC | 1 cut(s) | 482 |
| BcnI | CCSGG | 1 cut(s) | 564 |
| BfaI | CTAG | 2 cut(s) | 630, 680 |
| BfoI | RGCGCY | 1 cut(s) | 352 |
| BfuAI | ACCTGC | 1 cut(s) | 567 |
| BisI | GCNGC | 4 cut(s) | 45, 74, 485, 627 |
| BlpI | GCTNAGC | 1 cut(s) | 345 |
| BlsI | GCNGC | 4 cut(s) | 46, 75, 486, 628 |
| Bme1390I | CCNGG | 1 cut(s) | 564 |
| BmgBI | CACGTC | 1 cut(s) | 271 |
| BmgT120I | GGNCC | 1 cut(s) | 560 |
| BmiI | GGNNCC | 1 cut(s) | 561 |
| BmrFI | CCNGG | 1 cut(s) | 564 |
| BmsI | GCATC | 4 cut(s) | 31, 39, 487, 558 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 28 |
| BpmI | CTGGAG | 1 cut(s) | 170 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 345 |
| Bpu14I | TTCGAA | 1 cut(s) | 248 |
| BpuMI | CCSGG | 1 cut(s) | 564 |
| BsaBI | GATNNNNATC | 1 cut(s) | 113 |
| BsaJI | CCNNGG | 3 cut(s) | 401, 563, 655 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 380, 410 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 151, 448, 606, 656 |
| Bse118I | RCCGGY | 1 cut(s) | 63 |
| Bse1I | ACTGG | 2 cut(s) | 234, 322 |
| Bse8I | GATNNNNATC | 1 cut(s) | 113 |
| BseDI | CCNNGG | 3 cut(s) | 401, 563, 655 |
| BseGI | GGATG | 5 cut(s) | 165, 238, 331, 502, 573 |
| BseJI | GATNNNNATC | 1 cut(s) | 113 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 151, 448, 606, 656 |
| BseMII | CTCAG | 3 cut(s) | 198, 336, 525 |
| BseNI | ACTGG | 2 cut(s) | 234, 322 |
| BseRI | GAGGAG | 2 cut(s) | 401, 603 |
| BseXI | GCAGC | 3 cut(s) | 56, 85, 471 |
| BsgI | GTGCAG | 2 cut(s) | 92, 413 |
| BshFI | GGCC | 4 cut(s) | 330, 477, 562, 697 |
| BsiSI | CCGG | 3 cut(s) | 64, 86, 563 |
| BslFI | GGGAC | 1 cut(s) | 236 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 151, 448, 606, 656 |
| BsmFI | GGGAC | 1 cut(s) | 236 |
| BsmI | GAATGC | 1 cut(s) | 442 |
| BsnI | GGCC | 4 cut(s) | 330, 477, 562, 697 |
| Bsp119I | TTCGAA | 1 cut(s) | 248 |
| Bsp143I | GATC | 3 cut(s) | 114, 651, 661 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 345 |
| Bsp19I | CCATGG | 1 cut(s) | 655 |
| BspACI | CCGC | 6 cut(s) | 47, 176, 342, 571, 600, 626 |
| BspANI | GGCC | 4 cut(s) | 330, 477, 562, 697 |
| BspCNI | CTCAG | 3 cut(s) | 197, 337, 524 |
| BspHI | TCATGA | 1 cut(s) | 280 |
| BspLI | GGNNCC | 1 cut(s) | 561 |
| BspMI | ACCTGC | 1 cut(s) | 567 |
| BspPI | GGATC | 2 cut(s) | 109, 646 |
| BspT104I | TTCGAA | 1 cut(s) | 248 |
| BsrFI | RCCGGY | 1 cut(s) | 63 |
| BsrI | ACTGG | 2 cut(s) | 234, 322 |
| BssAI | RCCGGY | 1 cut(s) | 63 |
| BssECI | CCNNGG | 3 cut(s) | 401, 563, 655 |
| BssMI | GATC | 3 cut(s) | 114, 651, 661 |
| BssNAI | GTATAC | 1 cut(s) | 302 |
| BssSI | CACGAG | 1 cut(s) | 13 |
| BssT1I | CCWWGG | 1 cut(s) | 655 |
| Bst1107I | GTATAC | 1 cut(s) | 302 |
| Bst2BI | CACGAG | 1 cut(s) | 13 |
| Bst4CI | ACNGT | 4 cut(s) | 170, 356, 369, 402 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 58 |
| BstBI | TTCGAA | 1 cut(s) | 248 |
| BstC8I | GCNNGC | 1 cut(s) | 54 |
| BstDEI | CTNAG | 3 cut(s) | 184, 345, 511 |
| BstDSI | CCRYGG | 2 cut(s) | 401, 655 |
| BstF5I | GGATG | 5 cut(s) | 165, 238, 331, 502, 573 |
| BstH2I | RGCGCY | 1 cut(s) | 352 |
| BstHHI | GCGC | 2 cut(s) | 63, 351 |
| BstKTI | GATC | 3 cut(s) | 117, 654, 664 |
| BstMBI | GATC | 3 cut(s) | 114, 651, 661 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 53, 58, 82, 568 |
| BstSCI | CCNGG | 1 cut(s) | 562 |
| BstV1I | GCAGC | 3 cut(s) | 56, 85, 471 |
| BstX2I | RGATCY | 1 cut(s) | 114 |
| BstYI | RGATCY | 1 cut(s) | 114 |
| BstZ17I | GTATAC | 1 cut(s) | 302 |
| BsuRI | GGCC | 4 cut(s) | 330, 477, 562, 697 |
| BtgI | CCRYGG | 2 cut(s) | 401, 655 |
| BtrI | CACGTC | 1 cut(s) | 271 |
| BtsCI | GGATG | 5 cut(s) | 165, 238, 331, 502, 573 |
| BtsIMutI | CAGTG | 1 cut(s) | 365 |
| BveI | ACCTGC | 1 cut(s) | 567 |
| Cac8I | GCNNGC | 1 cut(s) | 54 |
| CciI | TCATGA | 1 cut(s) | 280 |
| CfoI | GCGC | 2 cut(s) | 63, 351 |
| Cfr10I | RCCGGY | 1 cut(s) | 63 |
| Cfr13I | GGNCC | 1 cut(s) | 560 |
| Csp6I | GTAC | 2 cut(s) | 357, 688 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 341, 376 |
| CviAII | CATG | 3 cut(s) | 281, 606, 656 |
| CviQI | GTAC | 2 cut(s) | 357, 688 |
| DdeI | CTNAG | 3 cut(s) | 184, 345, 511 |
| DpnI | GATC | 3 cut(s) | 116, 653, 663 |
| DpnII | GATC | 3 cut(s) | 114, 651, 661 |
| EaeI | YGGCCR | 1 cut(s) | 328 |
| Eco130I | CCWWGG | 1 cut(s) | 655 |
| Eco147I | AGGCCT | 1 cut(s) | 697 |
| EcoT14I | CCWWGG | 1 cut(s) | 655 |
| ErhI | CCWWGG | 1 cut(s) | 655 |
| FaeI | CATG | 3 cut(s) | 284, 609, 659 |
| FaiI | YATR | 9 cut(s) | 132, 134, 264, 282, 302, 327, 607, 657, 666 |
| FaqI | GGGAC | 1 cut(s) | 236 |
| FatI | CATG | 3 cut(s) | 280, 605, 655 |
| FblI | GTMKAC | 1 cut(s) | 301 |
| Fnu4HI | GCNGC | 4 cut(s) | 45, 74, 485, 627 |
| FokI | GGATG | 5 cut(s) | 172, 245, 318, 509, 580 |
| Fsp4HI | GCNGC | 4 cut(s) | 45, 74, 485, 627 |
| FspBI | CTAG | 2 cut(s) | 630, 680 |
| GlaI | GCGC | 2 cut(s) | 62, 350 |
| GluI | GCNGC | 4 cut(s) | 45, 74, 485, 627 |
| GsuI | CTGGAG | 1 cut(s) | 170 |
| HaeII | RGCGCY | 1 cut(s) | 352 |
| HaeIII | GGCC | 4 cut(s) | 330, 477, 562, 697 |
| HapII | CCGG | 3 cut(s) | 64, 86, 563 |
| HhaI | GCGC | 2 cut(s) | 63, 351 |
| Hin1II | CATG | 3 cut(s) | 284, 609, 659 |
| Hin6I | GCGC | 2 cut(s) | 61, 349 |
| HinP1I | GCGC | 2 cut(s) | 61, 349 |
| HincII | GTYRAC | 1 cut(s) | 315 |
| HindII | GTYRAC | 1 cut(s) | 315 |
| HpaI | GTTAAC | 1 cut(s) | 315 |
| HpaII | CCGG | 3 cut(s) | 64, 86, 563 |
| HphI | GGTGA | 2 cut(s) | 428, 470 |
| Hpy166II | GTNNAC | 3 cut(s) | 302, 315, 688 |
| Hpy188I | TCNGA | 2 cut(s) | 119, 514 |
| Hpy188III | TCNNGA | 2 cut(s) | 149, 281 |
| Hpy8I | GTNNAC | 3 cut(s) | 302, 315, 688 |
| Hpy99I | CGWCG | 1 cut(s) | 272 |
| HpyAV | CCTTC | 5 cut(s) | 442, 446, 467, 550, 687 |
| HpyCH4III | ACNGT | 4 cut(s) | 170, 356, 369, 402 |
| HpyCH4IV | ACGT | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 4 cut(s) | 44, 52, 73, 394 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 53, 58, 82, 568 |
| HpyF3I | CTNAG | 3 cut(s) | 184, 345, 511 |
| HpySE526I | ACGT | 1 cut(s) | 270 |
| Hsp92II | CATG | 3 cut(s) | 284, 609, 659 |
| HspAI | GCGC | 2 cut(s) | 61, 349 |
| KspAI | GTTAAC | 1 cut(s) | 315 |
| Kzo9I | GATC | 3 cut(s) | 114, 651, 661 |
| LmnI | GCTCC | 1 cut(s) | 573 |
| Lsp1109I | GCAGC | 3 cut(s) | 56, 85, 471 |
| LweI | GCATC | 4 cut(s) | 31, 39, 487, 558 |
| MaeI | CTAG | 2 cut(s) | 630, 680 |
| MaeII | ACGT | 1 cut(s) | 270 |
| MaeIII | GTNAC | 2 cut(s) | 363, 396 |
| MalI | GATC | 3 cut(s) | 116, 653, 663 |
| MboI | GATC | 3 cut(s) | 114, 651, 661 |
| MboII | GAAGA | 3 cut(s) | 124, 246, 526 |
| MflI | RGATCY | 1 cut(s) | 114 |
| MlsI | TGGCCA | 1 cut(s) | 330 |
| MluCI | AATT | 3 cut(s) | 19, 377, 515 |
| MluNI | TGGCCA | 1 cut(s) | 330 |
| MnlI | CCTC | 8 cut(s) | 145, 166, 213, 303, 379, 382, 449, 581 |
| Mox20I | TGGCCA | 1 cut(s) | 330 |
| MscI | TGGCCA | 1 cut(s) | 330 |
| MseI | TTAA | 1 cut(s) | 314 |
| Msp20I | TGGCCA | 1 cut(s) | 330 |
| MspA1I | CMGCKG | 3 cut(s) | 47, 344, 626 |
| MspI | CCGG | 3 cut(s) | 64, 86, 563 |
| MspR9I | CCNGG | 1 cut(s) | 564 |
| Mva1269I | GAATGC | 1 cut(s) | 442 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 53, 58, 82, 568 |
| NciI | CCSGG | 1 cut(s) | 564 |
| NcoI | CCATGG | 1 cut(s) | 655 |
| NdeII | GATC | 3 cut(s) | 114, 651, 661 |
| NlaIII | CATG | 3 cut(s) | 284, 609, 659 |
| NlaIV | GGNNCC | 1 cut(s) | 561 |
| NmuCI | GTSAC | 1 cut(s) | 363 |
| NspV | TTCGAA | 1 cut(s) | 248 |
| PagI | TCATGA | 1 cut(s) | 280 |
| PceI | AGGCCT | 1 cut(s) | 697 |
| PctI | GAATGC | 1 cut(s) | 442 |
| PkrI | GCNGC | 4 cut(s) | 46, 75, 486, 628 |
| PspN4I | GGNNCC | 1 cut(s) | 561 |
| PspPI | GGNCC | 1 cut(s) | 560 |
| PsuI | RGATCY | 1 cut(s) | 114 |
| RsaI | GTAC | 2 cut(s) | 358, 689 |
| RsaNI | GTAC | 2 cut(s) | 357, 688 |
| SaqAI | TTAA | 1 cut(s) | 314 |
| SatI | GCNGC | 4 cut(s) | 45, 74, 485, 627 |
| Sau3AI | GATC | 3 cut(s) | 114, 651, 661 |
| Sau96I | GGNCC | 1 cut(s) | 560 |
| ScrFI | CCNGG | 1 cut(s) | 564 |
| SetI | ASST | 9 cut(s) | 9, 156, 273, 314, 393, 432, 460, 581, 645 |
| SfaNI | GCATC | 4 cut(s) | 31, 39, 487, 558 |
| SfuI | TTCGAA | 1 cut(s) | 248 |
| Sse9I | AATT | 3 cut(s) | 19, 377, 515 |
| SseBI | AGGCCT | 1 cut(s) | 697 |
| SsiI | CCGC | 6 cut(s) | 47, 176, 342, 571, 600, 626 |
| SspMI | CTAG | 2 cut(s) | 630, 680 |
| StuI | AGGCCT | 1 cut(s) | 697 |
| StyD4I | CCNGG | 1 cut(s) | 562 |
| StyI | CCWWGG | 1 cut(s) | 655 |
| TaaI | ACNGT | 4 cut(s) | 170, 356, 369, 402 |
| TaiI | ACGT | 1 cut(s) | 273 |
| TaqI | TCGA | 3 cut(s) | 248, 267, 522 |
| TasI | AATT | 3 cut(s) | 19, 377, 515 |
| TauI | GCSGC | 1 cut(s) | 629 |
| Tru1I | TTAA | 1 cut(s) | 314 |
| Tru9I | TTAA | 1 cut(s) | 314 |
| TscAI | CASTG | 1 cut(s) | 372 |
| TseFI | GTSAC | 1 cut(s) | 363 |
| TseI | GCWGC | 3 cut(s) | 44, 73, 484 |
| Tsp45I | GTSAC | 1 cut(s) | 363 |
| TspDTI | ATGAA | 6 cut(s) | 125, 149, 179, 269, 297, 594 |
| TspGWI | ACGGA | 2 cut(s) | 134, 240 |
| TspRI | CASTG | 1 cut(s) | 372 |
| XmiI | GTMKAC | 1 cut(s) | 301 |
| XspI | CTAG | 2 cut(s) | 630, 680 |