Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 357 |
| AccII | CGCG | 1 cut(s) | 275 |
| AccIII | TCCGGA | 1 cut(s) | 197 |
| AciI | CCGC | 4 cut(s) | 275, 418, 533, 578 |
| AclWI | GGATC | 2 cut(s) | 295, 562 |
| AcyI | GRCGYC | 1 cut(s) | 354 |
| AfaI | GTAC | 2 cut(s) | 320, 484 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 48 |
| AgsI | TTSAA | 2 cut(s) | 61, 107 |
| AjnI | CCWGG | 1 cut(s) | 536 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 28 |
| Alw26I | GTCTC | 1 cut(s) | 304 |
| AlwI | GGATC | 2 cut(s) | 295, 562 |
| Aor13HI | TCCGGA | 1 cut(s) | 197 |
| AoxI | GGCC | 1 cut(s) | 534 |
| ApeKI | GCWGC | 1 cut(s) | 142 |
| AsuHPI | GGTGA | 1 cut(s) | 362 |
| BbsI | GAAGAC | 1 cut(s) | 68 |
| BbvI | GCAGC | 1 cut(s) | 129 |
| BccI | CCATC | 2 cut(s) | 383, 579 |
| BceAI | ACGGC | 1 cut(s) | 546 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 15, 49 |
| BciT130I | CCWGG | 1 cut(s) | 538 |
| BclI | TGATCA | 1 cut(s) | 163 |
| BcoDI | GTCTC | 1 cut(s) | 304 |
| BfaI | CTAG | 3 cut(s) | 155, 231, 515 |
| BfmI | CTRYAG | 1 cut(s) | 143 |
| BisI | GCNGC | 2 cut(s) | 143, 534 |
| BlsI | GCNGC | 2 cut(s) | 144, 535 |
| Bme1390I | CCNGG | 1 cut(s) | 538 |
| BmrFI | CCNGG | 1 cut(s) | 538 |
| BmrI | ACTGGG | 1 cut(s) | 576 |
| BmsI | GCATC | 1 cut(s) | 405 |
| BmuI | ACTGGG | 1 cut(s) | 576 |
| BpiI | GAAGAC | 1 cut(s) | 68 |
| BsaAI | YACGTR | 1 cut(s) | 318 |
| BsaHI | GRCGYC | 1 cut(s) | 354 |
| BsaJI | CCNNGG | 2 cut(s) | 25, 335 |
| BsaWI | WCCGGW | 1 cut(s) | 197 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 48 |
| Bse1I | ACTGG | 3 cut(s) | 304, 390, 582 |
| BseAI | TCCGGA | 1 cut(s) | 197 |
| BseBI | CCWGG | 1 cut(s) | 538 |
| BseDI | CCNNGG | 2 cut(s) | 25, 335 |
| BseGI | GGATG | 2 cut(s) | 6, 394 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 48 |
| BseMII | CTCAG | 1 cut(s) | 438 |
| BseNI | ACTGG | 3 cut(s) | 304, 390, 582 |
| BseXI | GCAGC | 1 cut(s) | 129 |
| Bsh1236I | CGCG | 1 cut(s) | 275 |
| Bsh1285I | CGRYCG | 1 cut(s) | 93 |
| BshFI | GGCC | 1 cut(s) | 536 |
| BsiEI | CGRYCG | 1 cut(s) | 93 |
| BsiSI | CCGG | 1 cut(s) | 198 |
| BsiWI | CGTACG | 1 cut(s) | 318 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 48 |
| BsmAI | GTCTC | 1 cut(s) | 304 |
| BsmI | GAATGC | 2 cut(s) | 370, 556 |
| BsnI | GGCC | 1 cut(s) | 536 |
| Bsp13I | TCCGGA | 1 cut(s) | 197 |
| Bsp143I | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| BspACI | CCGC | 4 cut(s) | 275, 418, 533, 578 |
| BspANI | GGCC | 1 cut(s) | 536 |
| BspCNI | CTCAG | 1 cut(s) | 439 |
| BspEI | TCCGGA | 1 cut(s) | 197 |
| BspFNI | CGCG | 1 cut(s) | 275 |
| BspMAI | CTGCAG | 1 cut(s) | 147 |
| BspPI | GGATC | 2 cut(s) | 295, 562 |
| BsrI | ACTGG | 3 cut(s) | 304, 390, 582 |
| BssECI | CCNNGG | 2 cut(s) | 25, 335 |
| BssMI | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| BssNI | GRCGYC | 1 cut(s) | 354 |
| BssT1I | CCWWGG | 1 cut(s) | 335 |
| Bst2UI | CCWGG | 1 cut(s) | 538 |
| Bst4CI | ACNGT | 2 cut(s) | 264, 410 |
| BstACI | GRCGYC | 1 cut(s) | 354 |
| BstBAI | YACGTR | 1 cut(s) | 318 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 87 |
| BstDEI | CTNAG | 3 cut(s) | 437, 447, 504 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 46 |
| BstF5I | GGATG | 2 cut(s) | 6, 394 |
| BstFNI | CGCG | 1 cut(s) | 275 |
| BstKTI | GATC | 5 cut(s) | 72, 93, 166, 303, 570 |
| BstMAI | GTCTC | 1 cut(s) | 304 |
| BstMBI | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| BstMCI | CGRYCG | 1 cut(s) | 93 |
| BstNI | CCWGG | 1 cut(s) | 538 |
| BstSCI | CCNGG | 1 cut(s) | 536 |
| BstSFI | CTRYAG | 1 cut(s) | 143 |
| BstSNI | TACGTA | 1 cut(s) | 318 |
| BstUI | CGCG | 1 cut(s) | 275 |
| BstV1I | GCAGC | 1 cut(s) | 129 |
| BstV2I | GAAGAC | 1 cut(s) | 68 |
| BsuRI | GGCC | 1 cut(s) | 536 |
| BtsCI | GGATG | 2 cut(s) | 6, 394 |
| BtsI | GCAGTG | 1 cut(s) | 152 |
| BtsIMutI | CAGTG | 2 cut(s) | 152, 311 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 87 |
| CseI | GACGC | 1 cut(s) | 281 |
| Csp6I | GTAC | 2 cut(s) | 319, 483 |
| CviAII | CATG | 3 cut(s) | 167, 257, 470 |
| CviJI | RGCY | 8 cut(s) | 85, 142, 297, 423, 434, 514, 536, 541 |
| CviKI_1 | RGCY | 8 cut(s) | 85, 142, 297, 423, 434, 514, 536, 541 |
| CviQI | GTAC | 2 cut(s) | 319, 483 |
| DdeI | CTNAG | 3 cut(s) | 437, 447, 504 |
| DpnI | GATC | 5 cut(s) | 71, 92, 165, 302, 569 |
| DpnII | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| Eco105I | TACGTA | 1 cut(s) | 318 |
| Eco130I | CCWWGG | 1 cut(s) | 335 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 46 |
| EcoRII | CCWGG | 1 cut(s) | 536 |
| EcoT14I | CCWWGG | 1 cut(s) | 335 |
| ErhI | CCWWGG | 1 cut(s) | 335 |
| FaeI | CATG | 3 cut(s) | 170, 260, 473 |
| FaiI | YATR | 5 cut(s) | 168, 240, 258, 444, 471 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 40, 72 |
| FatI | CATG | 3 cut(s) | 166, 256, 469 |
| FauI | CCCGC | 1 cut(s) | 411 |
| FbaI | TGATCA | 1 cut(s) | 163 |
| Fnu4HI | GCNGC | 2 cut(s) | 143, 534 |
| FokI | GGATG | 1 cut(s) | 401 |
| Fsp4HI | GCNGC | 2 cut(s) | 143, 534 |
| FspBI | CTAG | 3 cut(s) | 155, 231, 515 |
| GluI | GCNGC | 2 cut(s) | 143, 534 |
| HaeIII | GGCC | 1 cut(s) | 536 |
| HapII | CCGG | 1 cut(s) | 198 |
| HgaI | GACGC | 1 cut(s) | 281 |
| Hin1I | GRCGYC | 1 cut(s) | 354 |
| Hin1II | CATG | 3 cut(s) | 170, 260, 473 |
| HinfI | GANTC | 3 cut(s) | 151, 194, 494 |
| HpaII | CCGG | 1 cut(s) | 198 |
| HphI | GGTGA | 1 cut(s) | 362 |
| Hpy166II | GTNNAC | 3 cut(s) | 150, 283, 483 |
| Hpy188I | TCNGA | 6 cut(s) | 102, 119, 379, 414, 428, 499 |
| Hpy188III | TCNNGA | 4 cut(s) | 16, 198, 217, 561 |
| Hpy8I | GTNNAC | 3 cut(s) | 150, 283, 483 |
| HpyAV | CCTTC | 1 cut(s) | 118 |
| HpyCH4III | ACNGT | 2 cut(s) | 264, 410 |
| HpyCH4IV | ACGT | 2 cut(s) | 317, 354 |
| HpyCH4V | TGCA | 4 cut(s) | 36, 145, 170, 370 |
| HpyF3I | CTNAG | 3 cut(s) | 437, 447, 504 |
| HpySE526I | ACGT | 2 cut(s) | 317, 354 |
| Hsp92I | GRCGYC | 1 cut(s) | 354 |
| Hsp92II | CATG | 3 cut(s) | 170, 260, 473 |
| Kpn2I | TCCGGA | 1 cut(s) | 197 |
| Ksp22I | TGATCA | 1 cut(s) | 163 |
| Kzo9I | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| LmnI | GCTCC | 1 cut(s) | 489 |
| Lsp1109I | GCAGC | 1 cut(s) | 129 |
| LweI | GCATC | 1 cut(s) | 405 |
| MaeI | CTAG | 3 cut(s) | 155, 231, 515 |
| MaeII | ACGT | 2 cut(s) | 317, 354 |
| MaeIII | GTNAC | 2 cut(s) | 350, 526 |
| MalI | GATC | 5 cut(s) | 71, 92, 165, 302, 569 |
| MboI | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| MboII | GAAGA | 1 cut(s) | 73 |
| MluCI | AATT | 2 cut(s) | 19, 325 |
| MlyI | GAGTC | 1 cut(s) | 145 |
| MnlI | CCTC | 8 cut(s) | 20, 52, 132, 166, 194, 217, 262, 393 |
| MroI | TCCGGA | 1 cut(s) | 197 |
| MseI | TTAA | 1 cut(s) | 182 |
| MspI | CCGG | 1 cut(s) | 198 |
| MspR9I | CCNGG | 1 cut(s) | 538 |
| Mva1269I | GAATGC | 2 cut(s) | 370, 556 |
| MvaI | CCWGG | 1 cut(s) | 538 |
| MvnI | CGCG | 1 cut(s) | 275 |
| NdeII | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| NlaIII | CATG | 3 cut(s) | 170, 260, 473 |
| NmeAIII | GCCGAG | 1 cut(s) | 50 |
| NmuCI | GTSAC | 2 cut(s) | 350, 526 |
| PctI | GAATGC | 2 cut(s) | 370, 556 |
| PfeI | GAWTC | 2 cut(s) | 194, 494 |
| Pfl23II | CGTACG | 1 cut(s) | 318 |
| PkrI | GCNGC | 2 cut(s) | 144, 535 |
| Ple19I | CGATCG | 1 cut(s) | 93 |
| PleI | GAGTC | 1 cut(s) | 145 |
| PpsI | GAGTC | 1 cut(s) | 145 |
| Ppu21I | YACGTR | 1 cut(s) | 318 |
| Psp6I | CCWGG | 1 cut(s) | 536 |
| PspGI | CCWGG | 1 cut(s) | 536 |
| PspLI | CGTACG | 1 cut(s) | 318 |
| PstI | CTGCAG | 1 cut(s) | 147 |
| PvuI | CGATCG | 1 cut(s) | 93 |
| RsaI | GTAC | 2 cut(s) | 320, 484 |
| RsaNI | GTAC | 2 cut(s) | 319, 483 |
| SaqAI | TTAA | 1 cut(s) | 182 |
| SatI | GCNGC | 2 cut(s) | 143, 534 |
| Sau3AI | GATC | 5 cut(s) | 69, 90, 163, 300, 567 |
| SchI | GAGTC | 1 cut(s) | 145 |
| ScrFI | CCNGG | 1 cut(s) | 538 |
| SetI | ASST | 5 cut(s) | 320, 337, 357, 466, 510 |
| SfaNI | GCATC | 1 cut(s) | 405 |
| SfcI | CTRYAG | 1 cut(s) | 143 |
| SnaBI | TACGTA | 1 cut(s) | 318 |
| Sse9I | AATT | 2 cut(s) | 19, 325 |
| SsiI | CCGC | 4 cut(s) | 275, 418, 533, 578 |
| SspMI | CTAG | 3 cut(s) | 155, 231, 515 |
| StyD4I | CCNGG | 1 cut(s) | 536 |
| StyI | CCWWGG | 1 cut(s) | 335 |
| TaaI | ACNGT | 2 cut(s) | 264, 410 |
| TaiI | ACGT | 2 cut(s) | 320, 357 |
| TaqI | TCGA | 1 cut(s) | 299 |
| TasI | AATT | 2 cut(s) | 19, 325 |
| TatI | WGTACW | 1 cut(s) | 482 |
| TauI | GCSGC | 1 cut(s) | 536 |
| TfiI | GAWTC | 2 cut(s) | 194, 494 |
| Tru1I | TTAA | 1 cut(s) | 182 |
| Tru9I | TTAA | 1 cut(s) | 182 |
| TscAI | CASTG | 2 cut(s) | 152, 311 |
| TseFI | GTSAC | 2 cut(s) | 350, 526 |
| TseI | GCWGC | 1 cut(s) | 142 |
| Tsp45I | GTSAC | 2 cut(s) | 350, 526 |
| TspDTI | ATGAA | 5 cut(s) | 227, 256, 273, 363, 458 |
| TspRI | CASTG | 2 cut(s) | 152, 311 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 46 |
| XspI | CTAG | 3 cut(s) | 155, 231, 515 |
| ZraI | GACGTC | 1 cut(s) | 355 |