ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 6 |
| AccI | GTMKAC | 1 cut(s) | 1036 |
| AccII | CGCG | 4 cut(s) | 43, 54, 56, 1173 |
| AclWI | GGATC | 4 cut(s) | 301, 591, 969, 1443 |
| AcsI | RAATTY | 1 cut(s) | 1238 |
| AfaI | GTAC | 3 cut(s) | 795, 883, 1225 |
| AfeI | AGCGCT | 1 cut(s) | 813 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 199, 1046 |
| AflII | CTTAAG | 1 cut(s) | 404 |
| AgsI | TTSAA | 3 cut(s) | 493, 605, 1076 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1149 |
| AjnI | CCWGG | 1 cut(s) | 457 |
| Alw21I | GWGCWC | 1 cut(s) | 192 |
| Alw26I | GTCTC | 7 cut(s) | 151, 361, 608, 718, 925, 971, 1136 |
| AlwI | GGATC | 4 cut(s) | 301, 591, 969, 1443 |
| Ama87I | CYCGRG | 1 cut(s) | 1097 |
| Aor51HI | AGCGCT | 1 cut(s) | 813 |
| AoxI | GGCC | 3 cut(s) | 235, 798, 1100 |
| ApeKI | GCWGC | 4 cut(s) | 29, 32, 59, 110 |
| ApoI | RAATTY | 1 cut(s) | 1238 |
| AseI | ATTAAT | 1 cut(s) | 767 |
| AspLEI | GCGC | 4 cut(s) | 43, 56, 814, 1173 |
| AspS9I | GGNCC | 2 cut(s) | 527, 1100 |
| AsuC2I | CCSGG | 3 cut(s) | 563, 1098, 1099 |
| AsuHPI | GGTGA | 2 cut(s) | 271, 1002 |
| AsuII | TTCGAA | 2 cut(s) | 98, 833 |
| AvaI | CYCGRG | 1 cut(s) | 1097 |
| AvaII | GGWCC | 1 cut(s) | 527 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 848, 881 |
| BanII | GRGCYC | 1 cut(s) | 192 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 814, 846 |
| Bbv12I | GWGCWC | 1 cut(s) | 192 |
| BbvI | GCAGC | 4 cut(s) | 41, 44, 46, 122 |
| BccI | CCATC | 5 cut(s) | 250, 403, 475, 896, 1100 |
| BciT130I | CCWGG | 1 cut(s) | 459 |
| BciVI | GTATCC | 2 cut(s) | 703, 1092 |
| BcnI | CCSGG | 3 cut(s) | 563, 1098, 1099 |
| BcoDI | GTCTC | 7 cut(s) | 151, 361, 608, 718, 925, 971, 1136 |
| BfaI | CTAG | 7 cut(s) | 161, 590, 681, 758, 1221, 1332, 1350 |
| BfoI | RGCGCY | 1 cut(s) | 815 |
| BfrI | CTTAAG | 1 cut(s) | 404 |
| BfuI | GTATCC | 2 cut(s) | 703, 1092 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 56 |
| BisI | GCNGC | 8 cut(s) | 7, 30, 33, 36, 39, 57, 60, 111 |
| BlpI | GCTNAGC | 1 cut(s) | 1386 |
| BlsI | GCNGC | 8 cut(s) | 8, 31, 34, 37, 40, 58, 61, 112 |
| Bme1390I | CCNGG | 4 cut(s) | 459, 563, 1098, 1099 |
| Bme18I | GGWCC | 1 cut(s) | 527 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1149 |
| BmeT110I | CYCGRG | 1 cut(s) | 1097 |
| BmgT120I | GGNCC | 2 cut(s) | 527, 1100 |
| BmiI | GGNNCC | 1 cut(s) | 1339 |
| BmrFI | CCNGG | 4 cut(s) | 459, 563, 1098, 1099 |
| BmrI | ACTGGG | 1 cut(s) | 1147 |
| BmsI | GCATC | 4 cut(s) | 165, 220, 236, 436 |
| BmuI | ACTGGG | 1 cut(s) | 1147 |
| BpmI | CTGGAG | 1 cut(s) | 175 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1309 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1386 |
| Bpu14I | TTCGAA | 2 cut(s) | 98, 833 |
| BpuMI | CCSGG | 3 cut(s) | 563, 1098, 1099 |
| Bsa29I | ATCGAT | 1 cut(s) | 296 |
| BsaAI | YACGTR | 1 cut(s) | 885 |
| BsaI | GGTCTC | 1 cut(s) | 925 |
| BsaJI | CCNNGG | 2 cut(s) | 215, 1097 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 869, 899 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 199, 1046 |
| Bse1I | ACTGG | 2 cut(s) | 643, 1142 |
| BseBI | CCWGG | 1 cut(s) | 459 |
| BseCI | ATCGAT | 1 cut(s) | 296 |
| BseDI | CCNNGG | 2 cut(s) | 215, 1097 |
| BseGI | GGATG | 3 cut(s) | 180, 211, 1111 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 199, 1046 |
| BseMII | CTCAG | 3 cut(s) | 798, 1208, 1377 |
| BseNI | ACTGG | 2 cut(s) | 643, 1142 |
| BseRI | GAGGAG | 2 cut(s) | 890, 1067 |
| BseXI | GCAGC | 4 cut(s) | 41, 44, 46, 122 |
| BsgI | GTGCAG | 1 cut(s) | 129 |
| Bsh1236I | CGCG | 4 cut(s) | 43, 54, 56, 1173 |
| BshFI | GGCC | 3 cut(s) | 237, 800, 1102 |
| BshVI | ATCGAT | 1 cut(s) | 296 |
| BsiHKAI | GWGCWC | 1 cut(s) | 192 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1097 |
| BsiSI | CCGG | 4 cut(s) | 238, 562, 1098, 1103 |
| BslFI | GGGAC | 1 cut(s) | 629 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 199, 1046 |
| BsmAI | GTCTC | 7 cut(s) | 151, 361, 608, 718, 925, 971, 1136 |
| BsmFI | GGGAC | 1 cut(s) | 629 |
| BsnI | GGCC | 3 cut(s) | 237, 800, 1102 |
| Bso31I | GGTCTC | 1 cut(s) | 925 |
| BsoBI | CYCGRG | 1 cut(s) | 1097 |
| Bsp119I | TTCGAA | 2 cut(s) | 98, 833 |
| Bsp1286I | GDGCHC | 1 cut(s) | 192 |
| Bsp1407I | TGTACA | 1 cut(s) | 793 |
| Bsp143I | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1386 |
| BspANI | GGCC | 3 cut(s) | 237, 800, 1102 |
| BspCNI | CTCAG | 3 cut(s) | 797, 1207, 1378 |
| BspDI | ATCGAT | 1 cut(s) | 296 |
| BspFNI | CGCG | 4 cut(s) | 43, 54, 56, 1173 |
| BspHI | TCATGA | 1 cut(s) | 964 |
| BspLI | GGNNCC | 1 cut(s) | 1339 |
| BspPI | GGATC | 4 cut(s) | 301, 591, 969, 1443 |
| BspT104I | TTCGAA | 2 cut(s) | 98, 833 |
| BspTI | CTTAAG | 1 cut(s) | 404 |
| BspTNI | GGTCTC | 1 cut(s) | 925 |
| BsrBI | CCGCTC | 1 cut(s) | 6 |
| BsrGI | TGTACA | 1 cut(s) | 793 |
| BsrI | ACTGG | 2 cut(s) | 643, 1142 |
| BssECI | CCNNGG | 2 cut(s) | 215, 1097 |
| BssMI | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| BssT1I | CCWWGG | 1 cut(s) | 215 |
| Bst2UI | CCWGG | 1 cut(s) | 459 |
| Bst4CI | ACNGT | 4 cut(s) | 372, 442, 509, 527 |
| Bst6I | CTCTTC | 4 cut(s) | 328, 339, 928, 1371 |
| BstAFI | CTTAAG | 1 cut(s) | 404 |
| BstAUI | TGTACA | 1 cut(s) | 793 |
| BstBAI | YACGTR | 1 cut(s) | 885 |
| BstBI | TTCGAA | 2 cut(s) | 98, 833 |
| BstC8I | GCNNGC | 5 cut(s) | 52, 810, 1392, 1408, 1452 |
| BstDEI | CTNAG | 4 cut(s) | 784, 1194, 1309, 1386 |
| BstF5I | GGATG | 3 cut(s) | 180, 211, 1111 |
| BstFNI | CGCG | 4 cut(s) | 43, 54, 56, 1173 |
| BstH2I | RGCGCY | 1 cut(s) | 815 |
| BstHHI | GCGC | 4 cut(s) | 43, 56, 814, 1173 |
| BstKTI | GATC | 5 cut(s) | 296, 586, 672, 964, 1438 |
| BstMAI | GTCTC | 7 cut(s) | 151, 361, 608, 718, 925, 971, 1136 |
| BstMBI | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 35, 38, 47, 56, 1217, 1355, 1391, 1412 |
| BstNI | CCWGG | 1 cut(s) | 459 |
| BstNSI | RCATGY | 2 cut(s) | 253, 1394 |
| BstSCI | CCNGG | 4 cut(s) | 457, 561, 1096, 1097 |
| BstUI | CGCG | 4 cut(s) | 43, 54, 56, 1173 |
| BstV1I | GCAGC | 4 cut(s) | 41, 44, 46, 122 |
| BstX2I | RGATCY | 1 cut(s) | 583 |
| BstYI | RGATCY | 1 cut(s) | 583 |
| Bsu15I | ATCGAT | 1 cut(s) | 296 |
| BsuI | GTATCC | 2 cut(s) | 703, 1092 |
| BsuRI | GGCC | 3 cut(s) | 237, 800, 1102 |
| BsuTUI | ATCGAT | 1 cut(s) | 296 |
| BtgZI | GCGATG | 1 cut(s) | 259 |
| BtsCI | GGATG | 3 cut(s) | 180, 211, 1111 |
| BtsIMutI | CAGTG | 1 cut(s) | 438 |
| Cac8I | GCNNGC | 5 cut(s) | 52, 810, 1392, 1408, 1452 |
| CciI | TCATGA | 1 cut(s) | 964 |
| CfoI | GCGC | 4 cut(s) | 43, 56, 814, 1173 |
| Cfr13I | GGNCC | 2 cut(s) | 527, 1100 |
| Cfr9I | CCCGGG | 1 cut(s) | 1097 |
| ClaI | ATCGAT | 1 cut(s) | 296 |
| CseI | GACGC | 1 cut(s) | 195 |
| Csp6I | GTAC | 3 cut(s) | 794, 882, 1224 |
| CviAII | CATG | 6 cut(s) | 250, 736, 965, 1342, 1391, 1444 |
| CviQI | GTAC | 3 cut(s) | 794, 882, 1224 |
| DdeI | CTNAG | 4 cut(s) | 784, 1194, 1309, 1386 |
| DpnI | GATC | 5 cut(s) | 295, 585, 671, 963, 1437 |
| DpnII | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1149 |
| Eam1104I | CTCTTC | 4 cut(s) | 328, 339, 928, 1371 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1149 |
| EarI | CTCTTC | 4 cut(s) | 328, 339, 928, 1371 |
| EciI | GGCGGA | 1 cut(s) | 24 |
| Ecl136II | GAGCTC | 1 cut(s) | 190 |
| Eco130I | CCWWGG | 1 cut(s) | 215 |
| Eco24I | GRGCYC | 1 cut(s) | 192 |
| Eco31I | GGTCTC | 1 cut(s) | 925 |
| Eco47I | GGWCC | 1 cut(s) | 527 |
| Eco47III | AGCGCT | 1 cut(s) | 813 |
| Eco53kI | GAGCTC | 1 cut(s) | 190 |
| Eco88I | CYCGRG | 1 cut(s) | 1097 |
| EcoICRI | GAGCTC | 1 cut(s) | 190 |
| EcoRI | GAATTC | 1 cut(s) | 1238 |
| EcoRII | CCWGG | 1 cut(s) | 457 |
| EcoT14I | CCWWGG | 1 cut(s) | 215 |
| EcoT22I | ATGCAT | 1 cut(s) | 251 |
| EcoT38I | GRGCYC | 1 cut(s) | 192 |
| ErhI | CCWWGG | 1 cut(s) | 215 |
| FaeI | CATG | 6 cut(s) | 253, 739, 968, 1345, 1394, 1447 |
| FaqI | GGGAC | 1 cut(s) | 629 |
| FatI | CATG | 6 cut(s) | 249, 735, 964, 1341, 1390, 1443 |
| FauI | CCCGC | 4 cut(s) | 59, 75, 220, 368 |
| FauNDI | CATATG | 1 cut(s) | 432 |
| FblI | GTMKAC | 1 cut(s) | 1036 |
| Fnu4HI | GCNGC | 8 cut(s) | 7, 30, 33, 36, 39, 57, 60, 111 |
| FokI | GGATG | 3 cut(s) | 187, 198, 1118 |
| FriOI | GRGCYC | 1 cut(s) | 192 |
| Fsp4HI | GCNGC | 8 cut(s) | 7, 30, 33, 36, 39, 57, 60, 111 |
| FspBI | CTAG | 7 cut(s) | 161, 590, 681, 758, 1221, 1332, 1350 |
| GlaI | GCGC | 4 cut(s) | 42, 55, 813, 1172 |
| GluI | GCNGC | 8 cut(s) | 7, 30, 33, 36, 39, 57, 60, 111 |
| GsuI | CTGGAG | 1 cut(s) | 175 |
| HaeII | RGCGCY | 1 cut(s) | 815 |
| HaeIII | GGCC | 3 cut(s) | 237, 800, 1102 |
| HapII | CCGG | 4 cut(s) | 238, 562, 1098, 1103 |
| HgaI | GACGC | 1 cut(s) | 195 |
| HhaI | GCGC | 4 cut(s) | 43, 56, 814, 1173 |
| Hin1II | CATG | 6 cut(s) | 253, 739, 968, 1345, 1394, 1447 |
| Hin6I | GCGC | 4 cut(s) | 41, 54, 812, 1171 |
| HinP1I | GCGC | 4 cut(s) | 41, 54, 812, 1171 |
| HindIII | AAGCTT | 1 cut(s) | 828 |
| HinfI | GANTC | 4 cut(s) | 72, 146, 850, 1328 |
| HpaII | CCGG | 4 cut(s) | 238, 562, 1098, 1103 |
| HphI | GGTGA | 2 cut(s) | 271, 1002 |
| Hpy166II | GTNNAC | 4 cut(s) | 794, 945, 1037, 1249 |
| Hpy188I | TCNGA | 9 cut(s) | 71, 77, 117, 194, 280, 855, 955, 1204, 1278 |
| Hpy188III | TCNNGA | 6 cut(s) | 385, 590, 965, 1047, 1154, 1433 |
| Hpy8I | GTNNAC | 4 cut(s) | 794, 945, 1037, 1249 |
| Hpy99I | CGWCG | 1 cut(s) | 211 |
| HpyAV | CCTTC | 2 cut(s) | 164, 700 |
| HpyCH4III | ACNGT | 4 cut(s) | 372, 442, 509, 527 |
| HpyCH4IV | ACGT | 1 cut(s) | 884 |
| HpyCH4V | TGCA | 4 cut(s) | 110, 178, 249, 1394 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 35, 38, 47, 56, 1217, 1355, 1391, 1412 |
| HpyF3I | CTNAG | 4 cut(s) | 784, 1194, 1309, 1386 |
| HpySE526I | ACGT | 1 cut(s) | 884 |
| Hsp92II | CATG | 6 cut(s) | 253, 739, 968, 1345, 1394, 1447 |
| HspAI | GCGC | 4 cut(s) | 41, 54, 812, 1171 |
| Kzo9I | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| LmnI | GCTCC | 3 cut(s) | 11, 118, 195 |
| Lsp1109I | GCAGC | 4 cut(s) | 41, 44, 46, 122 |
| LweI | GCATC | 4 cut(s) | 165, 220, 236, 436 |
| MaeI | CTAG | 7 cut(s) | 161, 590, 681, 758, 1221, 1332, 1350 |
| MaeII | ACGT | 1 cut(s) | 884 |
| MaeIII | GTNAC | 3 cut(s) | 634, 658, 1280 |
| MalI | GATC | 5 cut(s) | 295, 585, 671, 963, 1437 |
| MbiI | CCGCTC | 1 cut(s) | 6 |
| MboI | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| MflI | RGATCY | 1 cut(s) | 583 |
| MhlI | GDGCHC | 1 cut(s) | 192 |
| MluCI | AATT | 5 cut(s) | 477, 519, 744, 764, 1238 |
| MmeI | TCCRAC | 6 cut(s) | 140, 258, 888, 939, 1153, 1256 |
| Mph1103I | ATGCAT | 1 cut(s) | 251 |
| MseI | TTAA | 6 cut(s) | 137, 405, 503, 596, 767, 1059 |
| MslI | CAYNNNNRTG | 2 cut(s) | 254, 1405 |
| MspA1I | CMGCKG | 2 cut(s) | 35, 1454 |
| MspCI | CTTAAG | 1 cut(s) | 404 |
| MspI | CCGG | 4 cut(s) | 238, 562, 1098, 1103 |
| MspR9I | CCNGG | 4 cut(s) | 459, 563, 1098, 1099 |
| MvaI | CCWGG | 1 cut(s) | 459 |
| MvnI | CGCG | 4 cut(s) | 43, 54, 56, 1173 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 35, 38, 47, 56, 1217, 1355, 1391, 1412 |
| NciI | CCSGG | 3 cut(s) | 563, 1098, 1099 |
| NdeI | CATATG | 1 cut(s) | 432 |
| NdeII | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| NlaIII | CATG | 6 cut(s) | 253, 739, 968, 1345, 1394, 1447 |
| NlaIV | GGNNCC | 1 cut(s) | 1339 |
| NmeAIII | GCCGAG | 1 cut(s) | 209 |
| NmuCI | GTSAC | 1 cut(s) | 1280 |
| NsiI | ATGCAT | 1 cut(s) | 251 |
| NspI | RCATGY | 2 cut(s) | 253, 1394 |
| NspV | TTCGAA | 2 cut(s) | 98, 833 |
| PaeI | GCATGC | 1 cut(s) | 1394 |
| PagI | TCATGA | 1 cut(s) | 964 |
| PfeI | GAWTC | 4 cut(s) | 72, 146, 850, 1328 |
| PfoI | TCCNGGA | 1 cut(s) | 561 |
| PkrI | GCNGC | 8 cut(s) | 8, 31, 34, 37, 40, 58, 61, 112 |
| Ppu21I | YACGTR | 1 cut(s) | 885 |
| PshBI | ATTAAT | 1 cut(s) | 767 |
| Psp124BI | GAGCTC | 1 cut(s) | 192 |
| Psp6I | CCWGG | 1 cut(s) | 457 |
| PspGI | CCWGG | 1 cut(s) | 457 |
| PspN4I | GGNNCC | 1 cut(s) | 1339 |
| PspPI | GGNCC | 2 cut(s) | 527, 1100 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 438, 470 |
| PsuI | RGATCY | 1 cut(s) | 583 |
| PvuII | CAGCTG | 1 cut(s) | 1454 |
| RsaI | GTAC | 3 cut(s) | 795, 883, 1225 |
| RsaNI | GTAC | 3 cut(s) | 794, 882, 1224 |
| RseI | CAYNNNNRTG | 2 cut(s) | 254, 1405 |
| SacI | GAGCTC | 1 cut(s) | 192 |
| SaqAI | TTAA | 6 cut(s) | 137, 405, 503, 596, 767, 1059 |
| SatI | GCNGC | 8 cut(s) | 7, 30, 33, 36, 39, 57, 60, 111 |
| Sau3AI | GATC | 5 cut(s) | 293, 583, 669, 961, 1435 |
| Sau96I | GGNCC | 2 cut(s) | 527, 1100 |
| ScrFI | CCNGG | 4 cut(s) | 459, 563, 1098, 1099 |
| SduI | GDGCHC | 1 cut(s) | 192 |
| SfaNI | GCATC | 4 cut(s) | 165, 220, 236, 436 |
| SfuI | TTCGAA | 2 cut(s) | 98, 833 |
| SinI | GGWCC | 1 cut(s) | 527 |
| SmaI | CCCGGG | 1 cut(s) | 1099 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 254, 1405 |
| SmlI | CTYRAG | 1 cut(s) | 404 |
| SmoI | CTYRAG | 1 cut(s) | 404 |
| SphI | GCATGC | 1 cut(s) | 1394 |
| SrfI | GCCCGGGC | 1 cut(s) | 1099 |
| Sse9I | AATT | 5 cut(s) | 477, 519, 744, 764, 1238 |
| SspI | AATATT | 1 cut(s) | 1188 |
| SspMI | CTAG | 7 cut(s) | 161, 590, 681, 758, 1221, 1332, 1350 |
| SstI | GAGCTC | 1 cut(s) | 192 |
| StyD4I | CCNGG | 4 cut(s) | 457, 561, 1096, 1097 |
| StyI | CCWWGG | 1 cut(s) | 215 |
| TaaI | ACNGT | 4 cut(s) | 372, 442, 509, 527 |
| TaiI | ACGT | 1 cut(s) | 887 |
| TaqI | TCGA | 8 cut(s) | 98, 144, 149, 168, 254, 296, 353, 833 |
| TasI | AATT | 5 cut(s) | 477, 519, 744, 764, 1238 |
| TatI | WGTACW | 2 cut(s) | 793, 1223 |
| TauI | GCSGC | 4 cut(s) | 9, 38, 41, 59 |
| TfiI | GAWTC | 4 cut(s) | 72, 146, 850, 1328 |
| Tru1I | TTAA | 6 cut(s) | 137, 405, 503, 596, 767, 1059 |
| Tru9I | TTAA | 6 cut(s) | 137, 405, 503, 596, 767, 1059 |
| TscAI | CASTG | 1 cut(s) | 445 |
| TseFI | GTSAC | 1 cut(s) | 1280 |
| TseI | GCWGC | 4 cut(s) | 29, 32, 59, 110 |
| Tsp45I | GTSAC | 1 cut(s) | 1280 |
| TspDTI | ATGAA | 4 cut(s) | 332, 915, 1034, 1460 |
| TspGWI | ACGGA | 2 cut(s) | 707, 1232 |
| TspMI | CCCGGG | 1 cut(s) | 1097 |
| TspRI | CASTG | 1 cut(s) | 445 |
| Vha464I | CTTAAG | 1 cut(s) | 404 |
| VpaK11BI | GGWCC | 1 cut(s) | 527 |
| VspI | ATTAAT | 1 cut(s) | 767 |
| XapI | RAATTY | 1 cut(s) | 1238 |
| XbaI | TCTAGA | 1 cut(s) | 589 |
| XceI | RCATGY | 2 cut(s) | 253, 1394 |
| XmaI | CCCGGG | 1 cut(s) | 1097 |
| XmiI | GTMKAC | 1 cut(s) | 1036 |
| XspI | CTAG | 7 cut(s) | 161, 590, 681, 758, 1221, 1332, 1350 |
| Zsp2I | ATGCAT | 1 cut(s) | 251 |