ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 824 |
| Acc16I | TGCGCA | 1 cut(s) | 168 |
| Acc36I | ACCTGC | 1 cut(s) | 773 |
| AccI | GTMKAC | 3 cut(s) | 656, 947, 968 |
| AccIII | TCCGGA | 1 cut(s) | 571 |
| AciI | CCGC | 2 cut(s) | 92, 1099 |
| AclWI | GGATC | 3 cut(s) | 78, 711, 1053 |
| AcsI | RAATTY | 4 cut(s) | 243, 373, 545, 1081 |
| AcyI | GRCGYC | 1 cut(s) | 206 |
| AfaI | GTAC | 3 cut(s) | 412, 505, 886 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 98, 420 |
| AgeI | ACCGGT | 1 cut(s) | 713 |
| AgsI | TTSAA | 6 cut(s) | 67, 238, 764, 1079, 1123, 1160 |
| AjiI | CACGTC | 1 cut(s) | 7 |
| AjnI | CCWGG | 1 cut(s) | 125 |
| AluBI | AGCT | 2 cut(s) | 269, 448 |
| AluI | AGCT | 2 cut(s) | 269, 448 |
| Alw21I | GWGCWC | 1 cut(s) | 144 |
| Alw26I | GTCTC | 3 cut(s) | 374, 465, 1002 |
| Alw44I | GTGCAC | 1 cut(s) | 140 |
| AlwI | GGATC | 3 cut(s) | 78, 711, 1053 |
| Aor13HI | TCCGGA | 1 cut(s) | 571 |
| AoxI | GGCC | 2 cut(s) | 89, 873 |
| ApaLI | GTGCAC | 1 cut(s) | 140 |
| ApeKI | GCWGC | 2 cut(s) | 490, 1070 |
| ApoI | RAATTY | 4 cut(s) | 243, 373, 545, 1081 |
| AseI | ATTAAT | 1 cut(s) | 1116 |
| AsiGI | ACCGGT | 1 cut(s) | 713 |
| AspLEI | GCGC | 2 cut(s) | 169, 625 |
| AspS9I | GGNCC | 4 cut(s) | 129, 272, 874, 1004 |
| AsuC2I | CCSGG | 1 cut(s) | 1002 |
| AsuHPI | GGTGA | 2 cut(s) | 206, 269 |
| AvaII | GGWCC | 3 cut(s) | 129, 272, 1004 |
| BaeGI | GKGCMC | 2 cut(s) | 144, 943 |
| Bbv12I | GWGCWC | 1 cut(s) | 144 |
| BbvCI | CCTCAGC | 1 cut(s) | 494 |
| BbvI | GCAGC | 2 cut(s) | 477, 1082 |
| BccI | CCATC | 7 cut(s) | 217, 462, 472, 575, 732, 913, 1004 |
| BciT130I | CCWGG | 1 cut(s) | 127 |
| BciVI | GTATCC | 1 cut(s) | 996 |
| BclI | TGATCA | 1 cut(s) | 663 |
| BcnI | CCSGG | 1 cut(s) | 1002 |
| BcoDI | GTCTC | 3 cut(s) | 374, 465, 1002 |
| BfaI | CTAG | 6 cut(s) | 108, 501, 618, 671, 890, 951 |
| BfoI | RGCGCY | 1 cut(s) | 626 |
| BfuAI | ACCTGC | 1 cut(s) | 773 |
| BfuI | GTATCC | 1 cut(s) | 996 |
| BglII | AGATCT | 1 cut(s) | 524 |
| BisI | GCNGC | 3 cut(s) | 92, 491, 1071 |
| BlsI | GCNGC | 3 cut(s) | 93, 492, 1072 |
| BmcAI | AGTACT | 1 cut(s) | 886 |
| Bme1390I | CCNGG | 2 cut(s) | 127, 1002 |
| Bme18I | GGWCC | 3 cut(s) | 129, 272, 1004 |
| BmgBI | CACGTC | 1 cut(s) | 7 |
| BmgT120I | GGNCC | 4 cut(s) | 129, 272, 874, 1004 |
| BmiI | GGNNCC | 2 cut(s) | 1005, 1006 |
| BmrFI | CCNGG | 2 cut(s) | 127, 1002 |
| BmrI | ACTGGG | 1 cut(s) | 326 |
| BmsI | GCATC | 4 cut(s) | 37, 178, 793, 1005 |
| BmuI | ACTGGG | 1 cut(s) | 326 |
| Bpu10I | CCTNAGC | 1 cut(s) | 494 |
| BpuEI | CTTGAG | 1 cut(s) | 1091 |
| BpuMI | CCSGG | 1 cut(s) | 1002 |
| Bsa29I | ATCGAT | 1 cut(s) | 538 |
| BsaAI | YACGTR | 1 cut(s) | 366 |
| BsaBI | GATNNNNATC | 1 cut(s) | 474 |
| BsaHI | GRCGYC | 1 cut(s) | 206 |
| BsaWI | WCCGGW | 2 cut(s) | 571, 713 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 982, 1012 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 98, 420 |
| Bse118I | RCCGGY | 1 cut(s) | 713 |
| Bse1I | ACTGG | 2 cut(s) | 321, 828 |
| Bse3DI | GCAATG | 2 cut(s) | 363, 383 |
| Bse8I | GATNNNNATC | 1 cut(s) | 474 |
| BseAI | TCCGGA | 1 cut(s) | 571 |
| BseBI | CCWGG | 1 cut(s) | 127 |
| BseCI | ATCGAT | 1 cut(s) | 538 |
| BseGI | GGATG | 4 cut(s) | 568, 805, 1020, 1066 |
| BseJI | GATNNNNATC | 1 cut(s) | 474 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 98, 420 |
| BseMI | GCAATG | 2 cut(s) | 363, 383 |
| BseMII | CTCAG | 2 cut(s) | 380, 485 |
| BseNI | ACTGG | 2 cut(s) | 321, 828 |
| BseRI | GAGGAG | 1 cut(s) | 313 |
| BseSI | GKGCMC | 2 cut(s) | 144, 943 |
| BseXI | GCAGC | 2 cut(s) | 477, 1082 |
| BshFI | GGCC | 2 cut(s) | 91, 875 |
| BshTI | ACCGGT | 1 cut(s) | 713 |
| BshVI | ATCGAT | 1 cut(s) | 538 |
| BsiHKAI | GWGCWC | 1 cut(s) | 144 |
| BsiSI | CCGG | 3 cut(s) | 572, 714, 1001 |
| BslFI | GGGAC | 2 cut(s) | 217, 1017 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 98, 420 |
| BsmAI | GTCTC | 3 cut(s) | 374, 465, 1002 |
| BsmFI | GGGAC | 2 cut(s) | 217, 1017 |
| BsnI | GGCC | 2 cut(s) | 91, 875 |
| Bsp1286I | GDGCHC | 2 cut(s) | 144, 943 |
| Bsp13I | TCCGGA | 1 cut(s) | 571 |
| Bsp143I | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| BspACI | CCGC | 2 cut(s) | 92, 1099 |
| BspANI | GGCC | 2 cut(s) | 91, 875 |
| BspCNI | CTCAG | 2 cut(s) | 381, 486 |
| BspDI | ATCGAT | 1 cut(s) | 538 |
| BspEI | TCCGGA | 1 cut(s) | 571 |
| BspLI | GGNNCC | 2 cut(s) | 1005, 1006 |
| BspMI | ACCTGC | 1 cut(s) | 773 |
| BspPI | GGATC | 3 cut(s) | 78, 711, 1053 |
| BsrDI | GCAATG | 2 cut(s) | 363, 383 |
| BsrFI | RCCGGY | 1 cut(s) | 713 |
| BsrI | ACTGG | 2 cut(s) | 321, 828 |
| BssAI | RCCGGY | 1 cut(s) | 713 |
| BssMI | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| BssNAI | GTATAC | 2 cut(s) | 657, 948 |
| BssNI | GRCGYC | 1 cut(s) | 206 |
| Bst1107I | GTATAC | 2 cut(s) | 657, 948 |
| Bst2UI | CCWGG | 1 cut(s) | 127 |
| Bst4CI | ACNGT | 2 cut(s) | 578, 936 |
| BstACI | GRCGYC | 1 cut(s) | 206 |
| BstBAI | YACGTR | 1 cut(s) | 366 |
| BstDEI | CTNAG | 6 cut(s) | 17, 278, 389, 494, 528, 642 |
| BstF5I | GGATG | 4 cut(s) | 568, 805, 1020, 1066 |
| BstH2I | RGCGCY | 1 cut(s) | 626 |
| BstHHI | GCGC | 2 cut(s) | 169, 625 |
| BstKTI | GATC | 7 cut(s) | 73, 99, 478, 527, 666, 706, 1048 |
| BstMAI | GTCTC | 3 cut(s) | 374, 465, 1002 |
| BstMBI | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 766 |
| BstNI | CCWGG | 1 cut(s) | 127 |
| BstSCI | CCNGG | 2 cut(s) | 125, 1000 |
| BstSLI | GKGCMC | 2 cut(s) | 144, 943 |
| BstV1I | GCAGC | 2 cut(s) | 477, 1082 |
| BstX2I | RGATCY | 3 cut(s) | 70, 524, 1045 |
| BstYI | RGATCY | 3 cut(s) | 70, 524, 1045 |
| BstZ17I | GTATAC | 2 cut(s) | 657, 948 |
| Bsu15I | ATCGAT | 1 cut(s) | 538 |
| BsuI | GTATCC | 1 cut(s) | 996 |
| BsuRI | GGCC | 2 cut(s) | 91, 875 |
| BsuTUI | ATCGAT | 1 cut(s) | 538 |
| BtrI | CACGTC | 1 cut(s) | 7 |
| BtsCI | GGATG | 4 cut(s) | 568, 805, 1020, 1066 |
| BtsIMutI | CAGTG | 1 cut(s) | 314 |
| BveI | ACCTGC | 1 cut(s) | 773 |
| CfoI | GCGC | 2 cut(s) | 169, 625 |
| Cfr10I | RCCGGY | 1 cut(s) | 713 |
| Cfr13I | GGNCC | 4 cut(s) | 129, 272, 874, 1004 |
| ClaI | ATCGAT | 1 cut(s) | 538 |
| CseI | GACGC | 4 cut(s) | 214, 605, 625, 843 |
| Csp6I | GTAC | 3 cut(s) | 411, 504, 885 |
| CspAI | ACCGGT | 1 cut(s) | 713 |
| CviAII | CATG | 6 cut(s) | 173, 350, 406, 420, 479, 1165 |
| CviQI | GTAC | 3 cut(s) | 411, 504, 885 |
| DdeI | CTNAG | 6 cut(s) | 17, 278, 389, 494, 528, 642 |
| DpnI | GATC | 7 cut(s) | 72, 98, 477, 526, 665, 705, 1047 |
| DpnII | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| DraI | TTTAAA | 2 cut(s) | 249, 544 |
| EciI | GGCGGA | 1 cut(s) | 1114 |
| Eco47I | GGWCC | 3 cut(s) | 129, 272, 1004 |
| EcoO109I | RGGNCCY | 2 cut(s) | 874, 1004 |
| EcoRII | CCWGG | 1 cut(s) | 125 |
| FaeI | CATG | 6 cut(s) | 176, 353, 409, 423, 482, 1168 |
| FaqI | GGGAC | 2 cut(s) | 217, 1017 |
| FatI | CATG | 6 cut(s) | 172, 349, 405, 419, 478, 1164 |
| FauNDI | CATATG | 1 cut(s) | 610 |
| FbaI | TGATCA | 1 cut(s) | 663 |
| FblI | GTMKAC | 3 cut(s) | 656, 947, 968 |
| Fnu4HI | GCNGC | 3 cut(s) | 92, 491, 1071 |
| FokI | GGATG | 4 cut(s) | 555, 812, 1027, 1073 |
| Fsp4HI | GCNGC | 3 cut(s) | 92, 491, 1071 |
| FspAI | RTGCGCAY | 1 cut(s) | 168 |
| FspBI | CTAG | 6 cut(s) | 108, 501, 618, 671, 890, 951 |
| FspI | TGCGCA | 1 cut(s) | 168 |
| GlaI | GCGC | 2 cut(s) | 168, 624 |
| GluI | GCNGC | 3 cut(s) | 92, 491, 1071 |
| HaeII | RGCGCY | 1 cut(s) | 626 |
| HaeIII | GGCC | 2 cut(s) | 91, 875 |
| HapII | CCGG | 3 cut(s) | 572, 714, 1001 |
| HgaI | GACGC | 4 cut(s) | 214, 605, 625, 843 |
| HhaI | GCGC | 2 cut(s) | 169, 625 |
| Hin1I | GRCGYC | 1 cut(s) | 206 |
| Hin1II | CATG | 6 cut(s) | 176, 353, 409, 423, 482, 1168 |
| Hin6I | GCGC | 2 cut(s) | 167, 623 |
| HinP1I | GCGC | 2 cut(s) | 167, 623 |
| HindIII | AAGCTT | 1 cut(s) | 446 |
| HinfI | GANTC | 4 cut(s) | 36, 197, 470, 1102 |
| HpaII | CCGG | 3 cut(s) | 572, 714, 1001 |
| HphI | GGTGA | 2 cut(s) | 206, 269 |
| Hpy166II | GTNNAC | 6 cut(s) | 80, 142, 411, 657, 948, 969 |
| Hpy188I | TCNGA | 7 cut(s) | 294, 469, 475, 708, 859, 981, 1050 |
| Hpy188III | TCNNGA | 6 cut(s) | 108, 308, 335, 572, 890, 1157 |
| Hpy8I | GTNNAC | 6 cut(s) | 80, 142, 411, 657, 948, 969 |
| Hpy99I | CGWCG | 1 cut(s) | 859 |
| HpyAV | CCTTC | 3 cut(s) | 61, 426, 758 |
| HpyCH4III | ACNGT | 2 cut(s) | 578, 936 |
| HpyCH4IV | ACGT | 3 cut(s) | 6, 289, 365 |
| HpyCH4V | TGCA | 5 cut(s) | 30, 142, 148, 565, 1070 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 766 |
| HpyF3I | CTNAG | 6 cut(s) | 17, 278, 389, 494, 528, 642 |
| HpySE526I | ACGT | 3 cut(s) | 6, 289, 365 |
| Hsp92I | GRCGYC | 1 cut(s) | 206 |
| Hsp92II | CATG | 6 cut(s) | 176, 353, 409, 423, 482, 1168 |
| HspAI | GCGC | 2 cut(s) | 167, 623 |
| KflI | GGGWCCC | 1 cut(s) | 1004 |
| Kpn2I | TCCGGA | 1 cut(s) | 571 |
| Ksp22I | TGATCA | 1 cut(s) | 663 |
| Kzo9I | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| LmnI | GCTCC | 1 cut(s) | 353 |
| Lsp1109I | GCAGC | 2 cut(s) | 477, 1082 |
| LweI | GCATC | 4 cut(s) | 37, 178, 793, 1005 |
| MaeI | CTAG | 6 cut(s) | 108, 501, 618, 671, 890, 951 |
| MaeII | ACGT | 3 cut(s) | 6, 289, 365 |
| MaeIII | GTNAC | 3 cut(s) | 437, 637, 910 |
| MalI | GATC | 7 cut(s) | 72, 98, 477, 526, 665, 705, 1047 |
| MboI | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| MboII | GAAGA | 3 cut(s) | 274, 712, 1021 |
| MfeI | CAATTG | 1 cut(s) | 897 |
| MflI | RGATCY | 3 cut(s) | 70, 524, 1045 |
| MhlI | GDGCHC | 2 cut(s) | 144, 943 |
| MlyI | GAGTC | 1 cut(s) | 206 |
| MmeI | TCCRAC | 3 cut(s) | 837, 1013, 1057 |
| MroI | TCCGGA | 1 cut(s) | 571 |
| MseI | TTAA | 4 cut(s) | 248, 543, 591, 1116 |
| MslI | CAYNNNNRTG | 1 cut(s) | 177 |
| MspI | CCGG | 3 cut(s) | 572, 714, 1001 |
| MspR9I | CCNGG | 2 cut(s) | 127, 1002 |
| MunI | CAATTG | 1 cut(s) | 897 |
| MvaI | CCWGG | 1 cut(s) | 127 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 766 |
| NciI | CCSGG | 1 cut(s) | 1002 |
| NdeI | CATATG | 1 cut(s) | 610 |
| NdeII | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| NlaIII | CATG | 6 cut(s) | 176, 353, 409, 423, 482, 1168 |
| NlaIV | GGNNCC | 2 cut(s) | 1005, 1006 |
| NmeAIII | GCCGAG | 1 cut(s) | 490 |
| NmuCI | GTSAC | 3 cut(s) | 437, 637, 910 |
| NsbI | TGCGCA | 1 cut(s) | 168 |
| PfeI | GAWTC | 3 cut(s) | 36, 470, 1102 |
| PfoI | TCCNGGA | 2 cut(s) | 125, 1000 |
| PinAI | ACCGGT | 1 cut(s) | 713 |
| PkrI | GCNGC | 3 cut(s) | 93, 492, 1072 |
| PleI | GAGTC | 1 cut(s) | 205 |
| PpsI | GAGTC | 1 cut(s) | 205 |
| Ppu21I | YACGTR | 1 cut(s) | 366 |
| PpuMI | RGGWCCY | 1 cut(s) | 1004 |
| PshBI | ATTAAT | 1 cut(s) | 1116 |
| PsiI | TTATAA | 1 cut(s) | 824 |
| Psp5II | RGGWCCY | 1 cut(s) | 1004 |
| Psp6I | CCWGG | 1 cut(s) | 125 |
| PspGI | CCWGG | 1 cut(s) | 125 |
| PspN4I | GGNNCC | 2 cut(s) | 1005, 1006 |
| PspPI | GGNCC | 4 cut(s) | 129, 272, 874, 1004 |
| PspPPI | RGGWCCY | 1 cut(s) | 1004 |
| PsuI | RGATCY | 3 cut(s) | 70, 524, 1045 |
| RsaI | GTAC | 3 cut(s) | 412, 505, 886 |
| RsaNI | GTAC | 3 cut(s) | 411, 504, 885 |
| RseI | CAYNNNNRTG | 1 cut(s) | 177 |
| SaqAI | TTAA | 4 cut(s) | 248, 543, 591, 1116 |
| SatI | GCNGC | 3 cut(s) | 92, 491, 1071 |
| Sau3AI | GATC | 7 cut(s) | 70, 96, 475, 524, 663, 703, 1045 |
| Sau96I | GGNCC | 4 cut(s) | 129, 272, 874, 1004 |
| ScaI | AGTACT | 1 cut(s) | 886 |
| SchI | GAGTC | 1 cut(s) | 206 |
| ScrFI | CCNGG | 2 cut(s) | 127, 1002 |
| SduI | GDGCHC | 2 cut(s) | 144, 943 |
| SfaNI | GCATC | 4 cut(s) | 37, 178, 793, 1005 |
| SinI | GGWCC | 3 cut(s) | 129, 272, 1004 |
| SmiI | ATTTAAAT | 1 cut(s) | 544 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 177 |
| SmlI | CTYRAG | 1 cut(s) | 1106 |
| SmoI | CTYRAG | 1 cut(s) | 1106 |
| SsiI | CCGC | 2 cut(s) | 92, 1099 |
| SspMI | CTAG | 6 cut(s) | 108, 501, 618, 671, 890, 951 |
| StyD4I | CCNGG | 2 cut(s) | 125, 1000 |
| SwaI | ATTTAAAT | 1 cut(s) | 544 |
| TaaI | ACNGT | 2 cut(s) | 578, 936 |
| TaiI | ACGT | 3 cut(s) | 9, 292, 368 |
| TaqI | TCGA | 4 cut(s) | 307, 336, 538, 549 |
| TaqII | GACCGA | 3 cut(s) | 89, 146, 898 |
| TatI | WGTACW | 2 cut(s) | 503, 884 |
| TauI | GCSGC | 1 cut(s) | 94 |
| TfiI | GAWTC | 3 cut(s) | 36, 470, 1102 |
| Tru1I | TTAA | 4 cut(s) | 248, 543, 591, 1116 |
| Tru9I | TTAA | 4 cut(s) | 248, 543, 591, 1116 |
| TscAI | CASTG | 1 cut(s) | 321 |
| TseFI | GTSAC | 3 cut(s) | 437, 637, 910 |
| TseI | GCWGC | 2 cut(s) | 490, 1070 |
| Tsp45I | GTSAC | 3 cut(s) | 437, 637, 910 |
| TspDTI | ATGAA | 4 cut(s) | 338, 408, 938, 1139 |
| TspRI | CASTG | 1 cut(s) | 321 |
| VneI | GTGCAC | 1 cut(s) | 140 |
| VpaK11BI | GGWCC | 3 cut(s) | 129, 272, 1004 |
| VspI | ATTAAT | 1 cut(s) | 1116 |
| XapI | RAATTY | 4 cut(s) | 243, 373, 545, 1081 |
| XbaI | TCTAGA | 2 cut(s) | 107, 889 |
| XmiI | GTMKAC | 3 cut(s) | 656, 947, 968 |
| XspI | CTAG | 6 cut(s) | 108, 501, 618, 671, 890, 951 |
| ZrmI | AGTACT | 1 cut(s) | 886 |