ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 229 |
| AccB1I | GGYRCC | 1 cut(s) | 258 |
| AccI | GTMKAC | 1 cut(s) | 497 |
| AciI | CCGC | 1 cut(s) | 491 |
| AclWI | GGATC | 3 cut(s) | 44, 373, 637 |
| AcyI | GRCGYC | 1 cut(s) | 259 |
| AfaI | GTAC | 2 cut(s) | 547, 640 |
| AgeI | ACCGGT | 1 cut(s) | 303 |
| AgsI | TTSAA | 4 cut(s) | 67, 373, 485, 586 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 346 |
| AjnI | CCWGG | 1 cut(s) | 554 |
| AluBI | AGCT | 2 cut(s) | 21, 316 |
| AluI | AGCT | 2 cut(s) | 21, 316 |
| Alw21I | GWGCWC | 1 cut(s) | 174 |
| Alw26I | GTCTC | 1 cut(s) | 658 |
| Alw44I | GTGCAC | 1 cut(s) | 170 |
| AlwI | GGATC | 3 cut(s) | 44, 373, 637 |
| Ama87I | CYCGRG | 1 cut(s) | 57 |
| AoxI | GGCC | 2 cut(s) | 488, 659 |
| ApaLI | GTGCAC | 1 cut(s) | 170 |
| ApeKI | GCWGC | 2 cut(s) | 313, 628 |
| AsiGI | ACCGGT | 1 cut(s) | 303 |
| AspLEI | GCGC | 2 cut(s) | 160, 261 |
| AspS9I | GGNCC | 5 cut(s) | 241, 301, 334, 407, 488 |
| AvaI | CYCGRG | 1 cut(s) | 57 |
| AvaII | GGWCC | 4 cut(s) | 241, 301, 334, 407 |
| BaeGI | GKGCMC | 1 cut(s) | 174 |
| BanI | GGYRCC | 1 cut(s) | 258 |
| Bbv12I | GWGCWC | 1 cut(s) | 174 |
| BbvI | GCAGC | 2 cut(s) | 325, 615 |
| BccI | CCATC | 2 cut(s) | 249, 398 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 135, 169 |
| BciT130I | CCWGG | 1 cut(s) | 556 |
| BcoDI | GTCTC | 1 cut(s) | 658 |
| BfaI | CTAG | 5 cut(s) | 161, 252, 414, 600, 636 |
| BfmI | CTRYAG | 1 cut(s) | 274 |
| BfoI | RGCGCY | 2 cut(s) | 161, 262 |
| BfuAI | ACCTGC | 1 cut(s) | 229 |
| BglII | AGATCT | 1 cut(s) | 70 |
| BisI | GCNGC | 3 cut(s) | 314, 491, 629 |
| BlsI | GCNGC | 3 cut(s) | 315, 492, 630 |
| Bme1390I | CCNGG | 1 cut(s) | 556 |
| Bme18I | GGWCC | 4 cut(s) | 241, 301, 334, 407 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 346 |
| BmeT110I | CYCGRG | 1 cut(s) | 57 |
| BmgT120I | GGNCC | 5 cut(s) | 241, 301, 334, 407, 488 |
| BmiI | GGNNCC | 3 cut(s) | 260, 302, 335 |
| BmrFI | CCNGG | 1 cut(s) | 556 |
| BpmI | CTGGAG | 1 cut(s) | 233 |
| BsaHI | GRCGYC | 1 cut(s) | 259 |
| BsaJI | CCNNGG | 1 cut(s) | 450 |
| BsaWI | WCCGGW | 1 cut(s) | 303 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 266, 296 |
| Bse118I | RCCGGY | 1 cut(s) | 303 |
| Bse3DI | GCAATG | 1 cut(s) | 49 |
| BseBI | CCWGG | 1 cut(s) | 556 |
| BseDI | CCNNGG | 1 cut(s) | 450 |
| BseGI | GGATG | 2 cut(s) | 238, 409 |
| BseMI | GCAATG | 1 cut(s) | 49 |
| BseMII | CTCAG | 2 cut(s) | 107, 320 |
| BseSI | GKGCMC | 1 cut(s) | 174 |
| BseXI | GCAGC | 2 cut(s) | 325, 615 |
| BsgI | GTGCAG | 1 cut(s) | 257 |
| Bsh1285I | CGRYCG | 1 cut(s) | 155 |
| BshFI | GGCC | 2 cut(s) | 490, 661 |
| BshNI | GGYRCC | 1 cut(s) | 258 |
| BshTI | ACCGGT | 1 cut(s) | 303 |
| BsiEI | CGRYCG | 1 cut(s) | 155 |
| BsiHKAI | GWGCWC | 1 cut(s) | 174 |
| BsiHKCI | CYCGRG | 1 cut(s) | 57 |
| BsiSI | CCGG | 3 cut(s) | 204, 304, 410 |
| BslFI | GGGAC | 3 cut(s) | 314, 347, 602 |
| BsmAI | GTCTC | 1 cut(s) | 658 |
| BsmFI | GGGAC | 3 cut(s) | 314, 347, 602 |
| BsnI | GGCC | 2 cut(s) | 490, 661 |
| BsoBI | CYCGRG | 1 cut(s) | 57 |
| Bsp1286I | GDGCHC | 1 cut(s) | 174 |
| Bsp1407I | TGTACA | 1 cut(s) | 545 |
| Bsp143I | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| BspACI | CCGC | 1 cut(s) | 491 |
| BspANI | GGCC | 2 cut(s) | 490, 661 |
| BspCNI | CTCAG | 2 cut(s) | 106, 321 |
| BspLI | GGNNCC | 3 cut(s) | 260, 302, 335 |
| BspMI | ACCTGC | 1 cut(s) | 229 |
| BspPI | GGATC | 3 cut(s) | 44, 373, 637 |
| BspT107I | GGYRCC | 1 cut(s) | 258 |
| BsrDI | GCAATG | 1 cut(s) | 49 |
| BsrFI | RCCGGY | 1 cut(s) | 303 |
| BsrGI | TGTACA | 1 cut(s) | 545 |
| BssAI | RCCGGY | 1 cut(s) | 303 |
| BssECI | CCNNGG | 1 cut(s) | 450 |
| BssMI | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| BssNI | GRCGYC | 1 cut(s) | 259 |
| BssT1I | CCWWGG | 1 cut(s) | 450 |
| Bst2UI | CCWGG | 1 cut(s) | 556 |
| Bst4CI | ACNGT | 2 cut(s) | 278, 669 |
| BstACI | GRCGYC | 1 cut(s) | 259 |
| BstAUI | TGTACA | 1 cut(s) | 545 |
| BstC8I | GCNNGC | 2 cut(s) | 209, 633 |
| BstDEI | CTNAG | 3 cut(s) | 17, 93, 329 |
| BstF5I | GGATG | 2 cut(s) | 238, 409 |
| BstH2I | RGCGCY | 2 cut(s) | 161, 262 |
| BstHHI | GCGC | 2 cut(s) | 160, 261 |
| BstKTI | GATC | 8 cut(s) | 39, 73, 99, 123, 328, 381, 613, 645 |
| BstMAI | GTCTC | 1 cut(s) | 658 |
| BstMBI | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| BstMCI | CGRYCG | 1 cut(s) | 155 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 27 |
| BstNI | CCWGG | 1 cut(s) | 556 |
| BstNSI | RCATGY | 1 cut(s) | 635 |
| BstSCI | CCNGG | 1 cut(s) | 554 |
| BstSFI | CTRYAG | 1 cut(s) | 274 |
| BstSLI | GKGCMC | 1 cut(s) | 174 |
| BstV1I | GCAGC | 2 cut(s) | 325, 615 |
| BstX2I | RGATCY | 3 cut(s) | 36, 70, 378 |
| BstYI | RGATCY | 3 cut(s) | 36, 70, 378 |
| BsuRI | GGCC | 2 cut(s) | 490, 661 |
| BtsCI | GGATG | 2 cut(s) | 238, 409 |
| BtsI | GCAGTG | 1 cut(s) | 546 |
| BtsIMutI | CAGTG | 1 cut(s) | 546 |
| BveI | ACCTGC | 1 cut(s) | 229 |
| Cac8I | GCNNGC | 2 cut(s) | 209, 633 |
| CfoI | GCGC | 2 cut(s) | 160, 261 |
| Cfr10I | RCCGGY | 1 cut(s) | 303 |
| Cfr13I | GGNCC | 5 cut(s) | 241, 301, 334, 407, 488 |
| CseI | GACGC | 1 cut(s) | 135 |
| Csp6I | GTAC | 2 cut(s) | 546, 639 |
| CspAI | ACCGGT | 1 cut(s) | 303 |
| CviAII | CATG | 5 cut(s) | 49, 309, 501, 614, 632 |
| CviJI | RGCY | 9 cut(s) | 21, 62, 91, 211, 316, 413, 449, 490, 661 |
| CviKI_1 | RGCY | 9 cut(s) | 21, 62, 91, 211, 316, 413, 449, 490, 661 |
| CviQI | GTAC | 2 cut(s) | 546, 639 |
| DdeI | CTNAG | 3 cut(s) | 17, 93, 329 |
| DinI | GGCGCC | 1 cut(s) | 260 |
| DpnI | GATC | 8 cut(s) | 38, 72, 98, 122, 327, 380, 612, 644 |
| DpnII | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| DriI | GACNNNNNGTC | 1 cut(s) | 346 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 346 |
| Eco130I | CCWWGG | 1 cut(s) | 450 |
| Eco47I | GGWCC | 4 cut(s) | 241, 301, 334, 407 |
| Eco88I | CYCGRG | 1 cut(s) | 57 |
| EcoO109I | RGGNCCY | 1 cut(s) | 334 |
| EcoRII | CCWGG | 1 cut(s) | 554 |
| EcoT14I | CCWWGG | 1 cut(s) | 450 |
| EgeI | GGCGCC | 1 cut(s) | 260 |
| EheI | GGCGCC | 1 cut(s) | 260 |
| ErhI | CCWWGG | 1 cut(s) | 450 |
| FaeI | CATG | 5 cut(s) | 52, 312, 504, 617, 635 |
| FaqI | GGGAC | 3 cut(s) | 314, 347, 602 |
| FatI | CATG | 5 cut(s) | 48, 308, 500, 613, 631 |
| FblI | GTMKAC | 1 cut(s) | 497 |
| Fnu4HI | GCNGC | 3 cut(s) | 314, 491, 629 |
| FokI | GGATG | 2 cut(s) | 245, 416 |
| Fsp4HI | GCNGC | 3 cut(s) | 314, 491, 629 |
| FspBI | CTAG | 5 cut(s) | 161, 252, 414, 600, 636 |
| GlaI | GCGC | 2 cut(s) | 159, 260 |
| GluI | GCNGC | 3 cut(s) | 314, 491, 629 |
| GsuI | CTGGAG | 1 cut(s) | 233 |
| HaeII | RGCGCY | 2 cut(s) | 161, 262 |
| HaeIII | GGCC | 2 cut(s) | 490, 661 |
| HapII | CCGG | 3 cut(s) | 204, 304, 410 |
| HgaI | GACGC | 1 cut(s) | 135 |
| HhaI | GCGC | 2 cut(s) | 160, 261 |
| Hin1I | GRCGYC | 1 cut(s) | 259 |
| Hin1II | CATG | 5 cut(s) | 52, 312, 504, 617, 635 |
| Hin6I | GCGC | 2 cut(s) | 158, 259 |
| HinP1I | GCGC | 2 cut(s) | 158, 259 |
| HincII | GTYRAC | 1 cut(s) | 498 |
| HindII | GTYRAC | 1 cut(s) | 498 |
| HinfI | GANTC | 3 cut(s) | 341, 551, 605 |
| HpaII | CCGG | 3 cut(s) | 204, 304, 410 |
| Hpy166II | GTNNAC | 4 cut(s) | 172, 464, 498, 546 |
| Hpy188I | TCNGA | 6 cut(s) | 96, 125, 330, 514, 572, 610 |
| Hpy188III | TCNNGA | 4 cut(s) | 223, 244, 443, 530 |
| Hpy8I | GTNNAC | 4 cut(s) | 172, 464, 498, 546 |
| HpyAV | CCTTC | 1 cut(s) | 394 |
| HpyCH4III | ACNGT | 2 cut(s) | 278, 669 |
| HpyCH4V | TGCA | 8 cut(s) | 30, 42, 146, 172, 238, 507, 578, 631 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 27 |
| HpyF3I | CTNAG | 3 cut(s) | 17, 93, 329 |
| Hsp92I | GRCGYC | 1 cut(s) | 259 |
| Hsp92II | CATG | 5 cut(s) | 52, 312, 504, 617, 635 |
| HspAI | GCGC | 2 cut(s) | 158, 259 |
| KasI | GGCGCC | 1 cut(s) | 258 |
| Kzo9I | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| Lsp1109I | GCAGC | 2 cut(s) | 325, 615 |
| MaeI | CTAG | 5 cut(s) | 161, 252, 414, 600, 636 |
| MaeIII | GTNAC | 2 cut(s) | 560, 602 |
| MalI | GATC | 8 cut(s) | 38, 72, 98, 122, 327, 380, 612, 644 |
| MboI | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| MboII | GAAGA | 1 cut(s) | 409 |
| MflI | RGATCY | 3 cut(s) | 36, 70, 378 |
| MhlI | GDGCHC | 1 cut(s) | 174 |
| MluCI | AATT | 1 cut(s) | 25 |
| Mly113I | GGCGCC | 1 cut(s) | 259 |
| MlyI | GAGTC | 2 cut(s) | 335, 599 |
| MnlI | CCTC | 7 cut(s) | 26, 102, 175, 223, 324, 347, 606 |
| MseI | TTAA | 1 cut(s) | 384 |
| MspI | CCGG | 3 cut(s) | 204, 304, 410 |
| MspR9I | CCNGG | 1 cut(s) | 556 |
| MvaI | CCWGG | 1 cut(s) | 556 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 27 |
| NarI | GGCGCC | 1 cut(s) | 259 |
| NdeII | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| NlaIII | CATG | 5 cut(s) | 52, 312, 504, 617, 635 |
| NlaIV | GGNNCC | 3 cut(s) | 260, 302, 335 |
| NmuCI | GTSAC | 2 cut(s) | 560, 602 |
| NspI | RCATGY | 1 cut(s) | 635 |
| PaeI | GCATGC | 1 cut(s) | 635 |
| PfeI | GAWTC | 1 cut(s) | 551 |
| PinAI | ACCGGT | 1 cut(s) | 303 |
| PkrI | GCNGC | 3 cut(s) | 315, 492, 630 |
| PleI | GAGTC | 2 cut(s) | 335, 599 |
| PluTI | GGCGCC | 1 cut(s) | 262 |
| PpsI | GAGTC | 2 cut(s) | 335, 599 |
| PpuMI | RGGWCCY | 1 cut(s) | 334 |
| Psp5II | RGGWCCY | 1 cut(s) | 334 |
| Psp6I | CCWGG | 1 cut(s) | 554 |
| PspGI | CCWGG | 1 cut(s) | 554 |
| PspN4I | GGNNCC | 3 cut(s) | 260, 302, 335 |
| PspPI | GGNCC | 5 cut(s) | 241, 301, 334, 407, 488 |
| PspPPI | RGGWCCY | 1 cut(s) | 334 |
| PsuI | RGATCY | 3 cut(s) | 36, 70, 378 |
| RsaI | GTAC | 2 cut(s) | 547, 640 |
| RsaNI | GTAC | 2 cut(s) | 546, 639 |
| SalI | GTCGAC | 1 cut(s) | 496 |
| SaqAI | TTAA | 1 cut(s) | 384 |
| SatI | GCNGC | 3 cut(s) | 314, 491, 629 |
| Sau3AI | GATC | 8 cut(s) | 36, 70, 96, 120, 325, 378, 610, 642 |
| Sau96I | GGNCC | 5 cut(s) | 241, 301, 334, 407, 488 |
| SchI | GAGTC | 2 cut(s) | 335, 599 |
| ScrFI | CCNGG | 1 cut(s) | 556 |
| SduI | GDGCHC | 1 cut(s) | 174 |
| SetI | ASST | 5 cut(s) | 23, 186, 243, 318, 339 |
| SfcI | CTRYAG | 1 cut(s) | 274 |
| SfoI | GGCGCC | 1 cut(s) | 260 |
| SinI | GGWCC | 4 cut(s) | 241, 301, 334, 407 |
| SphI | GCATGC | 1 cut(s) | 635 |
| Sse9I | AATT | 1 cut(s) | 25 |
| SsiI | CCGC | 1 cut(s) | 491 |
| SspDI | GGCGCC | 1 cut(s) | 258 |
| SspMI | CTAG | 5 cut(s) | 161, 252, 414, 600, 636 |
| StyD4I | CCNGG | 1 cut(s) | 554 |
| StyI | CCWWGG | 1 cut(s) | 450 |
| TaaI | ACNGT | 2 cut(s) | 278, 669 |
| TaqI | TCGA | 3 cut(s) | 339, 420, 497 |
| TaqII | GACCGA | 1 cut(s) | 141 |
| TasI | AATT | 1 cut(s) | 25 |
| TatI | WGTACW | 1 cut(s) | 545 |
| TauI | GCSGC | 1 cut(s) | 493 |
| TfiI | GAWTC | 1 cut(s) | 551 |
| Tru1I | TTAA | 1 cut(s) | 384 |
| Tru9I | TTAA | 1 cut(s) | 384 |
| TscAI | CASTG | 1 cut(s) | 546 |
| TseFI | GTSAC | 2 cut(s) | 560, 602 |
| TseI | GCWGC | 2 cut(s) | 313, 628 |
| Tsp45I | GTSAC | 2 cut(s) | 560, 602 |
| TspGWI | ACGGA | 1 cut(s) | 242 |
| TspRI | CASTG | 1 cut(s) | 546 |
| VneI | GTGCAC | 1 cut(s) | 170 |
| VpaK11BI | GGWCC | 4 cut(s) | 241, 301, 334, 407 |
| XceI | RCATGY | 1 cut(s) | 635 |
| XmiI | GTMKAC | 1 cut(s) | 497 |
| XspI | CTAG | 5 cut(s) | 161, 252, 414, 600, 636 |