Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 223 |
| Acc65I | GGTACC | 1 cut(s) | 245 |
| AccB1I | GGYRCC | 1 cut(s) | 245 |
| AccI | GTMKAC | 2 cut(s) | 121, 589 |
| AciI | CCGC | 1 cut(s) | 173 |
| AcsI | RAATTY | 2 cut(s) | 286, 494 |
| AcuI | CTGAAG | 1 cut(s) | 80 |
| AdeI | CACNNNGTG | 1 cut(s) | 416 |
| AfaI | GTAC | 2 cut(s) | 247, 556 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 339 |
| AflIII | ACRYGT | 1 cut(s) | 270 |
| AgsI | TTSAA | 4 cut(s) | 107, 239, 500, 515 |
| AjnI | CCWGG | 1 cut(s) | 154 |
| AluBI | AGCT | 5 cut(s) | 15, 212, 397, 548, 621 |
| AluI | AGCT | 5 cut(s) | 15, 212, 397, 548, 621 |
| Alw21I | GWGCWC | 1 cut(s) | 405 |
| Alw26I | GTCTC | 1 cut(s) | 463 |
| Alw44I | GTGCAC | 1 cut(s) | 401 |
| ApaLI | GTGCAC | 1 cut(s) | 401 |
| ApoI | RAATTY | 2 cut(s) | 286, 494 |
| Asp718I | GGTACC | 1 cut(s) | 245 |
| AsuHPI | GGTGA | 1 cut(s) | 402 |
| BaeGI | GKGCMC | 1 cut(s) | 405 |
| BanI | GGYRCC | 1 cut(s) | 245 |
| BanII | GRGCYC | 2 cut(s) | 58, 307 |
| Bbv12I | GWGCWC | 1 cut(s) | 405 |
| BccI | CCATC | 1 cut(s) | 373 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 382, 416 |
| BciT130I | CCWGG | 1 cut(s) | 156 |
| BcoDI | GTCTC | 1 cut(s) | 463 |
| BfaI | CTAG | 3 cut(s) | 51, 398, 414 |
| BfuAI | ACCTGC | 1 cut(s) | 223 |
| BmcAI | AGTACT | 1 cut(s) | 556 |
| Bme1390I | CCNGG | 1 cut(s) | 156 |
| BmiI | GGNNCC | 2 cut(s) | 247, 304 |
| BmrFI | CCNGG | 1 cut(s) | 156 |
| BmrI | ACTGGG | 1 cut(s) | 576 |
| BmsI | GCATC | 1 cut(s) | 268 |
| BmuI | ACTGGG | 1 cut(s) | 576 |
| BsaAI | YACGTR | 1 cut(s) | 446 |
| BsaBI | GATNNNNATC | 1 cut(s) | 423 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 339 |
| Bse1I | ACTGG | 1 cut(s) | 571 |
| Bse3DI | GCAATG | 1 cut(s) | 43 |
| Bse8I | GATNNNNATC | 1 cut(s) | 423 |
| BseBI | CCWGG | 1 cut(s) | 156 |
| BseGI | GGATG | 1 cut(s) | 517 |
| BseJI | GATNNNNATC | 1 cut(s) | 423 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 339 |
| BseMI | GCAATG | 1 cut(s) | 43 |
| BseMII | CTCAG | 1 cut(s) | 645 |
| BseNI | ACTGG | 1 cut(s) | 571 |
| BseSI | GKGCMC | 1 cut(s) | 405 |
| BsgI | GTGCAG | 1 cut(s) | 251 |
| BshNI | GGYRCC | 1 cut(s) | 245 |
| BsiHKAI | GWGCWC | 1 cut(s) | 405 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 339 |
| BsmAI | GTCTC | 1 cut(s) | 463 |
| BsmI | GAATGC | 2 cut(s) | 328, 503 |
| Bsp1286I | GDGCHC | 3 cut(s) | 58, 307, 405 |
| Bsp143I | GATC | 4 cut(s) | 69, 308, 610, 636 |
| BspACI | CCGC | 1 cut(s) | 173 |
| BspCNI | CTCAG | 1 cut(s) | 646 |
| BspLI | GGNNCC | 2 cut(s) | 247, 304 |
| BspMI | ACCTGC | 1 cut(s) | 223 |
| BspT107I | GGYRCC | 1 cut(s) | 245 |
| BsrDI | GCAATG | 1 cut(s) | 43 |
| BsrI | ACTGG | 1 cut(s) | 571 |
| BssMI | GATC | 4 cut(s) | 69, 308, 610, 636 |
| Bst2UI | CCWGG | 1 cut(s) | 156 |
| Bst4CI | ACNGT | 2 cut(s) | 342, 480 |
| Bst6I | CTCTTC | 1 cut(s) | 603 |
| BstBAI | YACGTR | 1 cut(s) | 446 |
| BstC8I | GCNNGC | 1 cut(s) | 451 |
| BstDEI | CTNAG | 2 cut(s) | 11, 654 |
| BstF5I | GGATG | 1 cut(s) | 517 |
| BstKTI | GATC | 4 cut(s) | 72, 311, 613, 639 |
| BstMAI | GTCTC | 1 cut(s) | 463 |
| BstMBI | GATC | 4 cut(s) | 69, 308, 610, 636 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 170 |
| BstNI | CCWGG | 1 cut(s) | 156 |
| BstNSI | RCATGY | 1 cut(s) | 274 |
| BstSCI | CCNGG | 1 cut(s) | 154 |
| BstSLI | GKGCMC | 1 cut(s) | 405 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 200 |
| BtsCI | GGATG | 1 cut(s) | 517 |
| BtsI | GCAGTG | 1 cut(s) | 140 |
| BtsIMutI | CAGTG | 1 cut(s) | 140 |
| BveI | ACCTGC | 1 cut(s) | 223 |
| Cac8I | GCNNGC | 1 cut(s) | 451 |
| Csp6I | GTAC | 2 cut(s) | 246, 555 |
| CviAII | CATG | 5 cut(s) | 205, 265, 271, 315, 649 |
| CviQI | GTAC | 2 cut(s) | 246, 555 |
| DdeI | CTNAG | 2 cut(s) | 11, 654 |
| DpnI | GATC | 4 cut(s) | 71, 310, 612, 638 |
| DpnII | GATC | 4 cut(s) | 69, 308, 610, 636 |
| DraI | TTTAAA | 1 cut(s) | 493 |
| DraIII | CACNNNGTG | 1 cut(s) | 416 |
| Eam1104I | CTCTTC | 1 cut(s) | 603 |
| EarI | CTCTTC | 1 cut(s) | 603 |
| Eco24I | GRGCYC | 2 cut(s) | 58, 307 |
| Eco57I | CTGAAG | 1 cut(s) | 80 |
| EcoRII | CCWGG | 1 cut(s) | 154 |
| EcoT22I | ATGCAT | 1 cut(s) | 261 |
| EcoT38I | GRGCYC | 2 cut(s) | 58, 307 |
| FaeI | CATG | 5 cut(s) | 208, 268, 274, 318, 652 |
| FatI | CATG | 5 cut(s) | 204, 264, 270, 314, 648 |
| FblI | GTMKAC | 2 cut(s) | 121, 589 |
| FokI | GGATG | 1 cut(s) | 504 |
| FriOI | GRGCYC | 2 cut(s) | 58, 307 |
| FspBI | CTAG | 3 cut(s) | 51, 398, 414 |
| Hin1II | CATG | 5 cut(s) | 208, 268, 274, 318, 652 |
| HincII | GTYRAC | 1 cut(s) | 122 |
| HindII | GTYRAC | 1 cut(s) | 122 |
| HindIII | AAGCTT | 2 cut(s) | 546, 619 |
| HinfI | GANTC | 3 cut(s) | 30, 85, 419 |
| HphI | GGTGA | 1 cut(s) | 402 |
| Hpy166II | GTNNAC | 3 cut(s) | 122, 403, 590 |
| Hpy188I | TCNGA | 2 cut(s) | 60, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 521 |
| Hpy8I | GTNNAC | 3 cut(s) | 122, 403, 590 |
| Hpy99I | CGWCG | 2 cut(s) | 477, 480 |
| HpyAV | CCTTC | 3 cut(s) | 55, 472, 565 |
| HpyCH4III | ACNGT | 2 cut(s) | 342, 480 |
| HpyCH4IV | ACGT | 1 cut(s) | 445 |
| HpyCH4V | TGCA | 8 cut(s) | 36, 145, 232, 259, 360, 403, 449, 528 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 170 |
| HpyF3I | CTNAG | 2 cut(s) | 11, 654 |
| HpySE526I | ACGT | 1 cut(s) | 445 |
| Hsp92II | CATG | 5 cut(s) | 208, 268, 274, 318, 652 |
| KpnI | GGTACC | 1 cut(s) | 249 |
| Kzo9I | GATC | 4 cut(s) | 69, 308, 610, 636 |
| LmnI | GCTCC | 3 cut(s) | 157, 209, 302 |
| LpnPI | CCDG | 8 cut(s) | 141, 168, 183, 218, 284, 493, 534, 552 |
| LweI | GCATC | 1 cut(s) | 268 |
| MaeI | CTAG | 3 cut(s) | 51, 398, 414 |
| MaeII | ACGT | 1 cut(s) | 445 |
| MaeIII | GTNAC | 2 cut(s) | 562, 644 |
| MalI | GATC | 4 cut(s) | 71, 310, 612, 638 |
| MboI | GATC | 4 cut(s) | 69, 308, 610, 636 |
| MboII | GAAGA | 2 cut(s) | 88, 590 |
| MhlI | GDGCHC | 3 cut(s) | 58, 307, 405 |
| MluCI | AATT | 4 cut(s) | 19, 90, 286, 494 |
| MlyI | GAGTC | 1 cut(s) | 79 |
| MnlI | CCTC | 3 cut(s) | 573, 608, 622 |
| Mph1103I | ATGCAT | 1 cut(s) | 261 |
| MseI | TTAA | 1 cut(s) | 492 |
| MslI | CAYNNNNRTG | 3 cut(s) | 254, 269, 275 |
| MspA1I | CMGCKG | 1 cut(s) | 173 |
| MspR9I | CCNGG | 1 cut(s) | 156 |
| Mva1269I | GAATGC | 2 cut(s) | 328, 503 |
| MvaI | CCWGG | 1 cut(s) | 156 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 170 |
| NdeII | GATC | 4 cut(s) | 69, 308, 610, 636 |
| NlaIII | CATG | 5 cut(s) | 208, 268, 274, 318, 652 |
| NlaIV | GGNNCC | 2 cut(s) | 247, 304 |
| NsiI | ATGCAT | 1 cut(s) | 261 |
| NspI | RCATGY | 1 cut(s) | 274 |
| PciI | ACATGT | 1 cut(s) | 270 |
| PctI | GAATGC | 2 cut(s) | 328, 503 |
| PfeI | GAWTC | 2 cut(s) | 30, 419 |
| PleI | GAGTC | 1 cut(s) | 79 |
| PpsI | GAGTC | 1 cut(s) | 79 |
| Ppu21I | YACGTR | 1 cut(s) | 446 |
| PscI | ACATGT | 1 cut(s) | 270 |
| Psp6I | CCWGG | 1 cut(s) | 154 |
| PspGI | CCWGG | 1 cut(s) | 154 |
| PspN4I | GGNNCC | 2 cut(s) | 247, 304 |
| RsaI | GTAC | 2 cut(s) | 247, 556 |
| RsaNI | GTAC | 2 cut(s) | 246, 555 |
| RseI | CAYNNNNRTG | 3 cut(s) | 254, 269, 275 |
| SalI | GTCGAC | 1 cut(s) | 120 |
| SaqAI | TTAA | 1 cut(s) | 492 |
| Sau3AI | GATC | 4 cut(s) | 69, 308, 610, 636 |
| ScaI | AGTACT | 1 cut(s) | 556 |
| SchI | GAGTC | 1 cut(s) | 79 |
| ScrFI | CCNGG | 1 cut(s) | 156 |
| SduI | GDGCHC | 3 cut(s) | 58, 307, 405 |
| SfaNI | GCATC | 1 cut(s) | 268 |
| SmiI | ATTTAAAT | 1 cut(s) | 493 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 254, 269, 275 |
| Sse9I | AATT | 4 cut(s) | 19, 90, 286, 494 |
| SsiI | CCGC | 1 cut(s) | 173 |
| SspI | AATATT | 1 cut(s) | 7 |
| SspMI | CTAG | 3 cut(s) | 51, 398, 414 |
| StyD4I | CCNGG | 1 cut(s) | 154 |
| SwaI | ATTTAAAT | 1 cut(s) | 493 |
| TaaI | ACNGT | 2 cut(s) | 342, 480 |
| TaiI | ACGT | 1 cut(s) | 448 |
| TaqI | TCGA | 3 cut(s) | 121, 375, 613 |
| TasI | AATT | 4 cut(s) | 19, 90, 286, 494 |
| TatI | WGTACW | 1 cut(s) | 554 |
| TfiI | GAWTC | 2 cut(s) | 30, 419 |
| Tru1I | TTAA | 1 cut(s) | 492 |
| Tru9I | TTAA | 1 cut(s) | 492 |
| TscAI | CASTG | 1 cut(s) | 147 |
| TspDTI | ATGAA | 3 cut(s) | 17, 411, 473 |
| TspRI | CASTG | 1 cut(s) | 147 |
| VneI | GTGCAC | 1 cut(s) | 401 |
| XapI | RAATTY | 2 cut(s) | 286, 494 |
| XceI | RCATGY | 1 cut(s) | 274 |
| XmiI | GTMKAC | 2 cut(s) | 121, 589 |
| XspI | CTAG | 3 cut(s) | 51, 398, 414 |
| ZrmI | AGTACT | 1 cut(s) | 556 |
| Zsp2I | ATGCAT | 1 cut(s) | 261 |