Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 262, 275 |
| AccIII | TCCGGA | 1 cut(s) | 197 |
| AciI | CCGC | 5 cut(s) | 148, 262, 275, 341, 484 |
| AclWI | GGATC | 2 cut(s) | 295, 304 |
| AfaI | GTAC | 1 cut(s) | 80 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 48 |
| AflIII | ACRYGT | 1 cut(s) | 317 |
| AgsI | TTSAA | 3 cut(s) | 61, 107, 424 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 28 |
| Alw26I | GTCTC | 1 cut(s) | 304 |
| AlwI | GGATC | 2 cut(s) | 295, 304 |
| Aor13HI | TCCGGA | 1 cut(s) | 197 |
| AoxI | GGCC | 1 cut(s) | 190 |
| ApeKI | GCWGC | 1 cut(s) | 145 |
| AspLEI | GCGC | 1 cut(s) | 262 |
| AsuC2I | CCSGG | 1 cut(s) | 494 |
| AsuHPI | GGTGA | 1 cut(s) | 428 |
| BanII | GRGCYC | 1 cut(s) | 470 |
| BbsI | GAAGAC | 1 cut(s) | 68 |
| BbvI | GCAGC | 1 cut(s) | 157 |
| BccI | CCATC | 1 cut(s) | 449 |
| BceAI | ACGGC | 1 cut(s) | 343 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 15, 49 |
| BclI | TGATCA | 1 cut(s) | 163 |
| BcnI | CCSGG | 1 cut(s) | 494 |
| BcoDI | GTCTC | 1 cut(s) | 304 |
| BfaI | CTAG | 3 cut(s) | 155, 231, 503 |
| BisI | GCNGC | 2 cut(s) | 146, 342 |
| BlsI | GCNGC | 2 cut(s) | 147, 343 |
| Bme1390I | CCNGG | 1 cut(s) | 494 |
| BmrFI | CCNGG | 1 cut(s) | 494 |
| BmsI | GCATC | 1 cut(s) | 471 |
| BoxI | GACNNNNGTC | 1 cut(s) | 355 |
| BpiI | GAAGAC | 1 cut(s) | 68 |
| BpuMI | CCSGG | 1 cut(s) | 494 |
| Bsa29I | ATCGAT | 1 cut(s) | 299 |
| BsaJI | CCNNGG | 2 cut(s) | 25, 401 |
| BsaWI | WCCGGW | 1 cut(s) | 197 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 48 |
| Bse1I | ACTGG | 2 cut(s) | 304, 456 |
| BseAI | TCCGGA | 1 cut(s) | 197 |
| BseCI | ATCGAT | 1 cut(s) | 299 |
| BseDI | CCNNGG | 2 cut(s) | 25, 401 |
| BseGI | GGATG | 3 cut(s) | 6, 144, 460 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 48 |
| BseMII | CTCAG | 1 cut(s) | 503 |
| BseNI | ACTGG | 2 cut(s) | 304, 456 |
| BseXI | GCAGC | 1 cut(s) | 157 |
| Bsh1236I | CGCG | 2 cut(s) | 262, 275 |
| BshFI | GGCC | 1 cut(s) | 192 |
| BshVI | ATCGAT | 1 cut(s) | 299 |
| BsiSI | CCGG | 2 cut(s) | 198, 493 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 48 |
| BsmAI | GTCTC | 1 cut(s) | 304 |
| BsmI | GAATGC | 1 cut(s) | 436 |
| BsnI | GGCC | 1 cut(s) | 192 |
| Bsp1286I | GDGCHC | 1 cut(s) | 470 |
| Bsp13I | TCCGGA | 1 cut(s) | 197 |
| Bsp143I | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| BspACI | CCGC | 5 cut(s) | 148, 262, 275, 341, 484 |
| BspANI | GGCC | 1 cut(s) | 192 |
| BspCNI | CTCAG | 1 cut(s) | 504 |
| BspDI | ATCGAT | 1 cut(s) | 299 |
| BspEI | TCCGGA | 1 cut(s) | 197 |
| BspFNI | CGCG | 2 cut(s) | 262, 275 |
| BspPI | GGATC | 2 cut(s) | 295, 304 |
| BsrI | ACTGG | 2 cut(s) | 304, 456 |
| BssECI | CCNNGG | 2 cut(s) | 25, 401 |
| BssMI | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| BssT1I | CCWWGG | 1 cut(s) | 401 |
| Bst4CI | ACNGT | 1 cut(s) | 78 |
| Bst6I | CTCTTC | 1 cut(s) | 123 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 87 |
| BstDEI | CTNAG | 1 cut(s) | 512 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 46 |
| BstF5I | GGATG | 3 cut(s) | 6, 144, 460 |
| BstFNI | CGCG | 2 cut(s) | 262, 275 |
| BstHHI | GCGC | 1 cut(s) | 262 |
| BstKTI | GATC | 5 cut(s) | 72, 117, 166, 299, 303 |
| BstMAI | GTCTC | 1 cut(s) | 304 |
| BstMBI | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| BstNSI | RCATGY | 3 cut(s) | 56, 260, 321 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 355 |
| BstSCI | CCNGG | 1 cut(s) | 492 |
| BstUI | CGCG | 2 cut(s) | 262, 275 |
| BstV1I | GCAGC | 1 cut(s) | 157 |
| BstV2I | GAAGAC | 1 cut(s) | 68 |
| Bsu15I | ATCGAT | 1 cut(s) | 299 |
| BsuRI | GGCC | 1 cut(s) | 192 |
| BsuTUI | ATCGAT | 1 cut(s) | 299 |
| BtsCI | GGATG | 3 cut(s) | 6, 144, 460 |
| BtsIMutI | CAGTG | 1 cut(s) | 311 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 87 |
| CfoI | GCGC | 1 cut(s) | 262 |
| ClaI | ATCGAT | 1 cut(s) | 299 |
| CseI | GACGC | 1 cut(s) | 281 |
| Csp6I | GTAC | 1 cut(s) | 79 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 341, 376 |
| CviAII | CATG | 4 cut(s) | 53, 167, 257, 318 |
| CviJI | RGCY | 6 cut(s) | 85, 192, 250, 468, 489, 500 |
| CviKI_1 | RGCY | 6 cut(s) | 85, 192, 250, 468, 489, 500 |
| CviQI | GTAC | 1 cut(s) | 79 |
| DdeI | CTNAG | 1 cut(s) | 512 |
| DpnI | GATC | 5 cut(s) | 71, 116, 165, 298, 302 |
| DpnII | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| Eam1104I | CTCTTC | 1 cut(s) | 123 |
| EarI | CTCTTC | 1 cut(s) | 123 |
| Eco130I | CCWWGG | 1 cut(s) | 401 |
| Eco24I | GRGCYC | 1 cut(s) | 470 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 46 |
| EcoT14I | CCWWGG | 1 cut(s) | 401 |
| EcoT38I | GRGCYC | 1 cut(s) | 470 |
| ErhI | CCWWGG | 1 cut(s) | 401 |
| FaeI | CATG | 4 cut(s) | 56, 170, 260, 321 |
| FaiI | YATR | 6 cut(s) | 54, 168, 240, 258, 319, 509 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 40, 72 |
| FatI | CATG | 4 cut(s) | 52, 166, 256, 317 |
| FauI | CCCGC | 1 cut(s) | 477 |
| FbaI | TGATCA | 1 cut(s) | 163 |
| Fnu4HI | GCNGC | 2 cut(s) | 146, 342 |
| FokI | GGATG | 2 cut(s) | 151, 467 |
| FriOI | GRGCYC | 1 cut(s) | 470 |
| Fsp4HI | GCNGC | 2 cut(s) | 146, 342 |
| FspBI | CTAG | 3 cut(s) | 155, 231, 503 |
| GlaI | GCGC | 1 cut(s) | 261 |
| GluI | GCNGC | 2 cut(s) | 146, 342 |
| HaeIII | GGCC | 1 cut(s) | 192 |
| HapII | CCGG | 2 cut(s) | 198, 493 |
| HgaI | GACGC | 1 cut(s) | 281 |
| HhaI | GCGC | 1 cut(s) | 262 |
| Hin1II | CATG | 4 cut(s) | 56, 170, 260, 321 |
| Hin6I | GCGC | 1 cut(s) | 260 |
| HinP1I | GCGC | 1 cut(s) | 260 |
| HinfI | GANTC | 4 cut(s) | 151, 356, 370, 392 |
| HpaII | CCGG | 2 cut(s) | 198, 493 |
| HphI | GGTGA | 1 cut(s) | 428 |
| Hpy166II | GTNNAC | 1 cut(s) | 283 |
| Hpy188I | TCNGA | 4 cut(s) | 102, 119, 445, 480 |
| Hpy188III | TCNNGA | 3 cut(s) | 16, 198, 217 |
| Hpy8I | GTNNAC | 1 cut(s) | 283 |
| HpyAV | CCTTC | 2 cut(s) | 118, 345 |
| HpyCH4III | ACNGT | 1 cut(s) | 78 |
| HpyCH4IV | ACGT | 1 cut(s) | 65 |
| HpyCH4V | TGCA | 4 cut(s) | 36, 145, 170, 436 |
| HpyF3I | CTNAG | 1 cut(s) | 512 |
| HpySE526I | ACGT | 1 cut(s) | 65 |
| Hsp92II | CATG | 4 cut(s) | 56, 170, 260, 321 |
| HspAI | GCGC | 1 cut(s) | 260 |
| Kpn2I | TCCGGA | 1 cut(s) | 197 |
| Ksp22I | TGATCA | 1 cut(s) | 163 |
| Kzo9I | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| LpnPI | CCDG | 8 cut(s) | 23, 99, 202, 211, 317, 437, 483, 506 |
| Lsp1109I | GCAGC | 1 cut(s) | 157 |
| LweI | GCATC | 1 cut(s) | 471 |
| MaeI | CTAG | 3 cut(s) | 155, 231, 503 |
| MaeII | ACGT | 1 cut(s) | 65 |
| MaeIII | GTNAC | 1 cut(s) | 416 |
| MalI | GATC | 5 cut(s) | 71, 116, 165, 298, 302 |
| MboI | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| MboII | GAAGA | 3 cut(s) | 73, 124, 140 |
| MhlI | GDGCHC | 1 cut(s) | 470 |
| MluCI | AATT | 1 cut(s) | 19 |
| MlyI | GAGTC | 2 cut(s) | 145, 365 |
| MnlI | CCTC | 4 cut(s) | 20, 52, 217, 262 |
| MroI | TCCGGA | 1 cut(s) | 197 |
| MseI | TTAA | 2 cut(s) | 182, 380 |
| MspA1I | CMGCKG | 1 cut(s) | 148 |
| MspI | CCGG | 2 cut(s) | 198, 493 |
| MspR9I | CCNGG | 1 cut(s) | 494 |
| Mva1269I | GAATGC | 1 cut(s) | 436 |
| MvnI | CGCG | 2 cut(s) | 262, 275 |
| NciI | CCSGG | 1 cut(s) | 494 |
| NdeII | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| NlaIII | CATG | 4 cut(s) | 56, 170, 260, 321 |
| NmeAIII | GCCGAG | 1 cut(s) | 50 |
| NmuCI | GTSAC | 1 cut(s) | 416 |
| NspI | RCATGY | 3 cut(s) | 56, 260, 321 |
| PaeI | GCATGC | 1 cut(s) | 56 |
| PciI | ACATGT | 1 cut(s) | 317 |
| PctI | GAATGC | 1 cut(s) | 436 |
| PfeI | GAWTC | 2 cut(s) | 370, 392 |
| PfoI | TCCNGGA | 1 cut(s) | 492 |
| PkrI | GCNGC | 2 cut(s) | 147, 343 |
| PleI | GAGTC | 2 cut(s) | 145, 364 |
| PpsI | GAGTC | 2 cut(s) | 145, 364 |
| PscI | ACATGT | 1 cut(s) | 317 |
| PshAI | GACNNNNGTC | 1 cut(s) | 355 |
| RsaI | GTAC | 1 cut(s) | 80 |
| RsaNI | GTAC | 1 cut(s) | 79 |
| SaqAI | TTAA | 2 cut(s) | 182, 380 |
| SatI | GCNGC | 2 cut(s) | 146, 342 |
| Sau3AI | GATC | 5 cut(s) | 69, 114, 163, 296, 300 |
| SchI | GAGTC | 2 cut(s) | 145, 365 |
| ScrFI | CCNGG | 1 cut(s) | 494 |
| SduI | GDGCHC | 1 cut(s) | 470 |
| SetI | ASST | 2 cut(s) | 68, 403 |
| SfaNI | GCATC | 1 cut(s) | 471 |
| SphI | GCATGC | 1 cut(s) | 56 |
| Sse9I | AATT | 1 cut(s) | 19 |
| SsiI | CCGC | 5 cut(s) | 148, 262, 275, 341, 484 |
| SspMI | CTAG | 3 cut(s) | 155, 231, 503 |
| StyD4I | CCNGG | 1 cut(s) | 492 |
| StyI | CCWWGG | 1 cut(s) | 401 |
| TaaI | ACNGT | 1 cut(s) | 78 |
| TaiI | ACGT | 1 cut(s) | 68 |
| TaqI | TCGA | 1 cut(s) | 299 |
| TaqII | GACCGA | 1 cut(s) | 363 |
| TasI | AATT | 1 cut(s) | 19 |
| TatI | WGTACW | 1 cut(s) | 78 |
| TauI | GCSGC | 1 cut(s) | 344 |
| TfiI | GAWTC | 2 cut(s) | 370, 392 |
| Tru1I | TTAA | 2 cut(s) | 182, 380 |
| Tru9I | TTAA | 2 cut(s) | 182, 380 |
| TscAI | CASTG | 1 cut(s) | 311 |
| TseFI | GTSAC | 1 cut(s) | 416 |
| TseI | GCWGC | 1 cut(s) | 145 |
| Tsp45I | GTSAC | 1 cut(s) | 416 |
| TspDTI | ATGAA | 4 cut(s) | 125, 227, 256, 429 |
| TspRI | CASTG | 1 cut(s) | 311 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 46 |
| XceI | RCATGY | 3 cut(s) | 56, 260, 321 |
| XspI | CTAG | 3 cut(s) | 155, 231, 503 |