ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 370 |
| AccII | CGCG | 2 cut(s) | 303, 634 |
| AciI | CCGC | 2 cut(s) | 632, 824 |
| AcoI | YGGCCR | 1 cut(s) | 541 |
| AcsI | RAATTY | 1 cut(s) | 478 |
| AdeI | CACNNNGTG | 1 cut(s) | 465 |
| AfaI | GTAC | 5 cut(s) | 176, 295, 469, 736, 782 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 550 |
| AflIII | ACRYGT | 1 cut(s) | 673 |
| AgsI | TTSAA | 4 cut(s) | 51, 355, 680, 687 |
| AluBI | AGCT | 3 cut(s) | 426, 609, 623 |
| AluI | AGCT | 3 cut(s) | 426, 609, 623 |
| Alw21I | GWGCWC | 1 cut(s) | 611 |
| Alw26I | GTCTC | 2 cut(s) | 107, 330 |
| AoxI | GGCC | 1 cut(s) | 541 |
| ApeKI | GCWGC | 3 cut(s) | 598, 842, 890 |
| ApoI | RAATTY | 1 cut(s) | 478 |
| AspLEI | GCGC | 3 cut(s) | 64, 303, 902 |
| AsuHPI | GGTGA | 2 cut(s) | 443, 838 |
| BalI | TGGCCA | 1 cut(s) | 543 |
| BanII | GRGCYC | 1 cut(s) | 611 |
| Bbv12I | GWGCWC | 1 cut(s) | 611 |
| BbvI | GCAGC | 3 cut(s) | 585, 854, 877 |
| BccI | CCATC | 2 cut(s) | 368, 725 |
| BclI | TGATCA | 1 cut(s) | 814 |
| BcoDI | GTCTC | 2 cut(s) | 107, 330 |
| BfaI | CTAG | 1 cut(s) | 549 |
| BfmI | CTRYAG | 2 cut(s) | 599, 710 |
| BfoI | RGCGCY | 2 cut(s) | 65, 903 |
| BfuAI | ACCTGC | 1 cut(s) | 370 |
| BglII | AGATCT | 1 cut(s) | 70 |
| BisI | GCNGC | 3 cut(s) | 599, 843, 891 |
| BlsI | GCNGC | 3 cut(s) | 600, 844, 892 |
| BmsI | GCATC | 2 cut(s) | 160, 852 |
| BpmI | CTGGAG | 1 cut(s) | 282 |
| Bsa29I | ATCGAT | 1 cut(s) | 372 |
| BsaAI | YACGTR | 1 cut(s) | 738 |
| BsaBI | GATNNNNATC | 2 cut(s) | 150, 813 |
| BsaJI | CCNNGG | 1 cut(s) | 320 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 550 |
| Bse1I | ACTGG | 1 cut(s) | 265 |
| Bse3DI | GCAATG | 1 cut(s) | 49 |
| Bse8I | GATNNNNATC | 2 cut(s) | 150, 813 |
| BseCI | ATCGAT | 1 cut(s) | 372 |
| BseDI | CCNNGG | 1 cut(s) | 320 |
| BseGI | GGATG | 2 cut(s) | 238, 754 |
| BseJI | GATNNNNATC | 2 cut(s) | 150, 813 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 550 |
| BseMI | GCAATG | 1 cut(s) | 49 |
| BseMII | CTCAG | 2 cut(s) | 179, 858 |
| BseNI | ACTGG | 1 cut(s) | 265 |
| BseXI | GCAGC | 3 cut(s) | 585, 854, 877 |
| BseYI | CCCAGC | 1 cut(s) | 569 |
| BsgI | GTGCAG | 1 cut(s) | 786 |
| Bsh1236I | CGCG | 2 cut(s) | 303, 634 |
| Bsh1285I | CGRYCG | 1 cut(s) | 632 |
| BshFI | GGCC | 1 cut(s) | 543 |
| BshVI | ATCGAT | 1 cut(s) | 372 |
| BsiEI | CGRYCG | 1 cut(s) | 632 |
| BsiHKAI | GWGCWC | 1 cut(s) | 611 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 550 |
| BsmAI | GTCTC | 2 cut(s) | 107, 330 |
| BsnI | GGCC | 1 cut(s) | 543 |
| Bsp1286I | GDGCHC | 1 cut(s) | 611 |
| Bsp143I | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| BspACI | CCGC | 2 cut(s) | 632, 824 |
| BspANI | GGCC | 1 cut(s) | 543 |
| BspCNI | CTCAG | 2 cut(s) | 180, 859 |
| BspDI | ATCGAT | 1 cut(s) | 372 |
| BspFNI | CGCG | 2 cut(s) | 303, 634 |
| BspMAI | CTGCAG | 1 cut(s) | 603 |
| BspMI | ACCTGC | 1 cut(s) | 370 |
| BsrDI | GCAATG | 1 cut(s) | 49 |
| BsrI | ACTGG | 1 cut(s) | 265 |
| BssECI | CCNNGG | 1 cut(s) | 320 |
| BssMI | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| BssT1I | CCWWGG | 1 cut(s) | 320 |
| Bst4CI | ACNGT | 1 cut(s) | 359 |
| Bst6I | CTCTTC | 1 cut(s) | 900 |
| BstBAI | YACGTR | 1 cut(s) | 738 |
| BstC8I | GCNNGC | 2 cut(s) | 603, 607 |
| BstDEI | CTNAG | 5 cut(s) | 17, 188, 422, 789, 867 |
| BstF5I | GGATG | 2 cut(s) | 238, 754 |
| BstFNI | CGCG | 2 cut(s) | 303, 634 |
| BstH2I | RGCGCY | 2 cut(s) | 65, 903 |
| BstHHI | GCGC | 3 cut(s) | 64, 303, 902 |
| BstKTI | GATC | 7 cut(s) | 73, 274, 307, 372, 436, 445, 817 |
| BstMAI | GTCTC | 2 cut(s) | 107, 330 |
| BstMBI | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| BstMCI | CGRYCG | 1 cut(s) | 632 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 715, 899 |
| BstNSI | RCATGY | 2 cut(s) | 677, 709 |
| BstSFI | CTRYAG | 2 cut(s) | 599, 710 |
| BstUI | CGCG | 2 cut(s) | 303, 634 |
| BstV1I | GCAGC | 3 cut(s) | 585, 854, 877 |
| BstX2I | RGATCY | 1 cut(s) | 70 |
| BstYI | RGATCY | 1 cut(s) | 70 |
| Bsu15I | ATCGAT | 1 cut(s) | 372 |
| BsuRI | GGCC | 1 cut(s) | 543 |
| BsuTUI | ATCGAT | 1 cut(s) | 372 |
| BtgZI | GCGATG | 2 cut(s) | 230, 626 |
| BtsCI | GGATG | 2 cut(s) | 238, 754 |
| BtsI | GCAGTG | 1 cut(s) | 314 |
| BtsIMutI | CAGTG | 1 cut(s) | 314 |
| BveI | ACCTGC | 1 cut(s) | 370 |
| Cac8I | GCNNGC | 2 cut(s) | 603, 607 |
| CfoI | GCGC | 3 cut(s) | 64, 303, 902 |
| ClaI | ATCGAT | 1 cut(s) | 372 |
| CseI | GACGC | 1 cut(s) | 623 |
| Csp6I | GTAC | 5 cut(s) | 175, 294, 468, 735, 781 |
| CviAII | CATG | 9 cut(s) | 279, 455, 525, 545, 584, 674, 706, 760, 806 |
| CviJI | RGCY | 6 cut(s) | 389, 426, 543, 609, 623, 718 |
| CviKI_1 | RGCY | 6 cut(s) | 389, 426, 543, 609, 623, 718 |
| CviQI | GTAC | 5 cut(s) | 175, 294, 468, 735, 781 |
| DdeI | CTNAG | 5 cut(s) | 17, 188, 422, 789, 867 |
| DpnI | GATC | 7 cut(s) | 72, 273, 306, 371, 435, 444, 816 |
| DpnII | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| DraI | TTTAAA | 1 cut(s) | 343 |
| DraIII | CACNNNGTG | 1 cut(s) | 465 |
| EaeI | YGGCCR | 1 cut(s) | 541 |
| Eam1104I | CTCTTC | 1 cut(s) | 900 |
| EarI | CTCTTC | 1 cut(s) | 900 |
| Ecl136II | GAGCTC | 1 cut(s) | 609 |
| Eco130I | CCWWGG | 1 cut(s) | 320 |
| Eco24I | GRGCYC | 1 cut(s) | 611 |
| Eco53kI | GAGCTC | 1 cut(s) | 609 |
| EcoICRI | GAGCTC | 1 cut(s) | 609 |
| EcoRI | GAATTC | 1 cut(s) | 478 |
| EcoT14I | CCWWGG | 1 cut(s) | 320 |
| EcoT38I | GRGCYC | 1 cut(s) | 611 |
| ErhI | CCWWGG | 1 cut(s) | 320 |
| FaeI | CATG | 9 cut(s) | 282, 458, 528, 548, 587, 677, 709, 763, 809 |
| FatI | CATG | 9 cut(s) | 278, 454, 524, 544, 583, 673, 705, 759, 805 |
| FbaI | TGATCA | 1 cut(s) | 814 |
| Fnu4HI | GCNGC | 3 cut(s) | 599, 843, 891 |
| FokI | GGATG | 2 cut(s) | 245, 761 |
| FriOI | GRGCYC | 1 cut(s) | 611 |
| Fsp4HI | GCNGC | 3 cut(s) | 599, 843, 891 |
| FspBI | CTAG | 1 cut(s) | 549 |
| GlaI | GCGC | 3 cut(s) | 63, 302, 901 |
| GluI | GCNGC | 3 cut(s) | 599, 843, 891 |
| GsaI | CCCAGC | 1 cut(s) | 573 |
| GsuI | CTGGAG | 1 cut(s) | 282 |
| HaeII | RGCGCY | 2 cut(s) | 65, 903 |
| HaeIII | GGCC | 1 cut(s) | 543 |
| HgaI | GACGC | 1 cut(s) | 623 |
| HhaI | GCGC | 3 cut(s) | 64, 303, 902 |
| Hin1II | CATG | 9 cut(s) | 282, 458, 528, 548, 587, 677, 709, 763, 809 |
| Hin6I | GCGC | 3 cut(s) | 62, 301, 900 |
| HinP1I | GCGC | 3 cut(s) | 62, 301, 900 |
| HinfI | GANTC | 5 cut(s) | 47, 311, 575, 613, 680 |
| HphI | GGTGA | 2 cut(s) | 443, 838 |
| Hpy166II | GTNNAC | 2 cut(s) | 468, 798 |
| Hpy188I | TCNGA | 4 cut(s) | 171, 449, 484, 580 |
| Hpy188III | TCNNGA | 3 cut(s) | 308, 413, 818 |
| Hpy8I | GTNNAC | 2 cut(s) | 468, 798 |
| HpyCH4III | ACNGT | 1 cut(s) | 359 |
| HpyCH4IV | ACGT | 1 cut(s) | 737 |
| HpyCH4V | TGCA | 8 cut(s) | 30, 42, 151, 254, 601, 670, 803, 842 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 715, 899 |
| HpyF3I | CTNAG | 5 cut(s) | 17, 188, 422, 789, 867 |
| HpySE526I | ACGT | 1 cut(s) | 737 |
| Hsp92II | CATG | 9 cut(s) | 282, 458, 528, 548, 587, 677, 709, 763, 809 |
| HspAI | GCGC | 3 cut(s) | 62, 301, 900 |
| Ksp22I | TGATCA | 1 cut(s) | 814 |
| Kzo9I | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| LmnI | GCTCC | 1 cut(s) | 592 |
| LpnPI | CCDG | 8 cut(s) | 246, 293, 365, 490, 583, 587, 698, 879 |
| Lsp1109I | GCAGC | 3 cut(s) | 585, 854, 877 |
| LweI | GCATC | 2 cut(s) | 160, 852 |
| MaeI | CTAG | 1 cut(s) | 549 |
| MaeII | ACGT | 1 cut(s) | 737 |
| MalI | GATC | 7 cut(s) | 72, 273, 306, 371, 435, 444, 816 |
| MboI | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| MboII | GAAGA | 5 cut(s) | 379, 554, 675, 740, 917 |
| MfeI | CAATTG | 1 cut(s) | 491 |
| MflI | RGATCY | 1 cut(s) | 70 |
| MhlI | GDGCHC | 1 cut(s) | 611 |
| MlsI | TGGCCA | 1 cut(s) | 543 |
| MluCI | AATT | 6 cut(s) | 25, 96, 199, 478, 491, 881 |
| MluNI | TGGCCA | 1 cut(s) | 543 |
| Mox20I | TGGCCA | 1 cut(s) | 543 |
| MscI | TGGCCA | 1 cut(s) | 543 |
| MseI | TTAA | 3 cut(s) | 342, 648, 912 |
| MslI | CAYNNNNRTG | 2 cut(s) | 791, 810 |
| Msp20I | TGGCCA | 1 cut(s) | 543 |
| MunI | CAATTG | 1 cut(s) | 491 |
| MvnI | CGCG | 2 cut(s) | 303, 634 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 715, 899 |
| NdeII | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| NlaIII | CATG | 9 cut(s) | 282, 458, 528, 548, 587, 677, 709, 763, 809 |
| NspI | RCATGY | 2 cut(s) | 677, 709 |
| PciI | ACATGT | 1 cut(s) | 673 |
| PfeI | GAWTC | 5 cut(s) | 47, 311, 575, 613, 680 |
| PflFI | GACNNNGTC | 1 cut(s) | 633 |
| PkrI | GCNGC | 3 cut(s) | 600, 844, 892 |
| Ppu21I | YACGTR | 1 cut(s) | 738 |
| PscI | ACATGT | 1 cut(s) | 673 |
| Psp124BI | GAGCTC | 1 cut(s) | 611 |
| PspFI | CCCAGC | 1 cut(s) | 569 |
| PstI | CTGCAG | 1 cut(s) | 603 |
| PsuI | RGATCY | 1 cut(s) | 70 |
| PsyI | GACNNNGTC | 1 cut(s) | 633 |
| RsaI | GTAC | 5 cut(s) | 176, 295, 469, 736, 782 |
| RsaNI | GTAC | 5 cut(s) | 175, 294, 468, 735, 781 |
| RseI | CAYNNNNRTG | 2 cut(s) | 791, 810 |
| SacI | GAGCTC | 1 cut(s) | 611 |
| SaqAI | TTAA | 3 cut(s) | 342, 648, 912 |
| SatI | GCNGC | 3 cut(s) | 599, 843, 891 |
| Sau3AI | GATC | 7 cut(s) | 70, 271, 304, 369, 433, 442, 814 |
| SduI | GDGCHC | 1 cut(s) | 611 |
| SetI | ASST | 9 cut(s) | 37, 183, 384, 428, 611, 625, 740, 779, 856 |
| SfaNI | GCATC | 2 cut(s) | 160, 852 |
| SfcI | CTRYAG | 2 cut(s) | 599, 710 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 791, 810 |
| Sse9I | AATT | 6 cut(s) | 25, 96, 199, 478, 491, 881 |
| SsiI | CCGC | 2 cut(s) | 632, 824 |
| SspI | AATATT | 1 cut(s) | 769 |
| SspMI | CTAG | 1 cut(s) | 549 |
| SstI | GAGCTC | 1 cut(s) | 611 |
| StyI | CCWWGG | 1 cut(s) | 320 |
| TaaI | ACNGT | 1 cut(s) | 359 |
| TaiI | ACGT | 1 cut(s) | 740 |
| TaqI | TCGA | 3 cut(s) | 127, 372, 611 |
| TasI | AATT | 6 cut(s) | 25, 96, 199, 478, 491, 881 |
| TatI | WGTACW | 3 cut(s) | 174, 293, 467 |
| TfiI | GAWTC | 5 cut(s) | 47, 311, 575, 613, 680 |
| Tru1I | TTAA | 3 cut(s) | 342, 648, 912 |
| Tru9I | TTAA | 3 cut(s) | 342, 648, 912 |
| TscAI | CASTG | 1 cut(s) | 321 |
| TseI | GCWGC | 3 cut(s) | 598, 842, 890 |
| TspDTI | ATGAA | 6 cut(s) | 17, 267, 443, 548, 741, 887 |
| TspRI | CASTG | 1 cut(s) | 321 |
| Tth111I | GACNNNGTC | 1 cut(s) | 633 |
| XapI | RAATTY | 1 cut(s) | 478 |
| XceI | RCATGY | 2 cut(s) | 677, 709 |
| XspI | CTAG | 1 cut(s) | 549 |