ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 264 |
| Acc36I | ACCTGC | 1 cut(s) | 869 |
| AccBSI | CCGCTC | 1 cut(s) | 1096 |
| AccI | GTMKAC | 1 cut(s) | 353 |
| AciI | CCGC | 3 cut(s) | 170, 1041, 1094 |
| AclWI | GGATC | 2 cut(s) | 44, 741 |
| AcsI | RAATTY | 2 cut(s) | 364, 443 |
| AcuI | CTGAAG | 2 cut(s) | 86, 1161 |
| AfaI | GTAC | 3 cut(s) | 237, 403, 1002 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1078 |
| AgsI | TTSAA | 6 cut(s) | 177, 442, 464, 766, 911, 1052 |
| AjiI | CACGTC | 1 cut(s) | 511 |
| AluBI | AGCT | 5 cut(s) | 305, 568, 590, 691, 789 |
| AluI | AGCT | 5 cut(s) | 305, 568, 590, 691, 789 |
| Alw21I | GWGCWC | 1 cut(s) | 631 |
| Alw26I | GTCTC | 3 cut(s) | 107, 1069, 1095 |
| AlwI | GGATC | 2 cut(s) | 44, 741 |
| Ama87I | CYCGRG | 1 cut(s) | 1072 |
| ApeKI | GCWGC | 3 cut(s) | 536, 604, 806 |
| ApoI | RAATTY | 2 cut(s) | 364, 443 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 827, 1146 |
| AspLEI | GCGC | 2 cut(s) | 64, 265 |
| AspS9I | GGNCC | 1 cut(s) | 1038 |
| AsuHPI | GGTGA | 2 cut(s) | 329, 1045 |
| AvaI | CYCGRG | 1 cut(s) | 1072 |
| AvaII | GGWCC | 1 cut(s) | 1038 |
| Bbv12I | GWGCWC | 1 cut(s) | 631 |
| BbvI | GCAGC | 3 cut(s) | 548, 616, 793 |
| BccI | CCATC | 1 cut(s) | 188 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 21, 55 |
| BciVI | GTATCC | 2 cut(s) | 555, 1068 |
| BcoDI | GTCTC | 3 cut(s) | 107, 1069, 1095 |
| BfaI | CTAG | 2 cut(s) | 600, 647 |
| BfoI | RGCGCY | 1 cut(s) | 65 |
| BfuAI | ACCTGC | 1 cut(s) | 869 |
| BfuI | GTATCC | 2 cut(s) | 555, 1068 |
| BisI | GCNGC | 3 cut(s) | 537, 605, 807 |
| BlsI | GCNGC | 3 cut(s) | 538, 606, 808 |
| Bme18I | GGWCC | 1 cut(s) | 1038 |
| BmeT110I | CYCGRG | 1 cut(s) | 1072 |
| BmgBI | CACGTC | 1 cut(s) | 511 |
| BmgT120I | GGNCC | 1 cut(s) | 1038 |
| BmsI | GCATC | 2 cut(s) | 160, 274 |
| BoxI | GACNNNNGTC | 1 cut(s) | 879 |
| BpmI | CTGGAG | 1 cut(s) | 365 |
| BpuEI | CTTGAG | 1 cut(s) | 164 |
| BsaI | GGTCTC | 1 cut(s) | 1069 |
| BsaJI | CCNNGG | 1 cut(s) | 31 |
| BsaWI | WCCGGW | 1 cut(s) | 1078 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1078 |
| Bse1I | ACTGG | 3 cut(s) | 382, 461, 520 |
| Bse3DI | GCAATG | 2 cut(s) | 49, 999 |
| BseDI | CCNNGG | 1 cut(s) | 31 |
| BseGI | GGATG | 2 cut(s) | 88, 1087 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1078 |
| BseMI | GCAATG | 2 cut(s) | 49, 999 |
| BseNI | ACTGG | 3 cut(s) | 382, 461, 520 |
| BseRI | GAGGAG | 1 cut(s) | 1043 |
| BseXI | GCAGC | 3 cut(s) | 548, 616, 793 |
| BsgI | GTGCAG | 3 cut(s) | 555, 692, 1149 |
| BsiHKAI | GWGCWC | 1 cut(s) | 631 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1072 |
| BsiSI | CCGG | 1 cut(s) | 1079 |
| BslFI | GGGAC | 1 cut(s) | 1130 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1078 |
| BsmAI | GTCTC | 3 cut(s) | 107, 1069, 1095 |
| BsmBI | CGTCTC | 1 cut(s) | 107 |
| BsmFI | GGGAC | 1 cut(s) | 1130 |
| BsmI | GAATGC | 1 cut(s) | 172 |
| Bso31I | GGTCTC | 1 cut(s) | 1069 |
| BsoBI | CYCGRG | 1 cut(s) | 1072 |
| Bsp1286I | GDGCHC | 1 cut(s) | 631 |
| Bsp1407I | TGTACA | 3 cut(s) | 235, 401, 1000 |
| Bsp143I | GATC | 4 cut(s) | 36, 120, 388, 746 |
| BspACI | CCGC | 3 cut(s) | 170, 1041, 1094 |
| BspMI | ACCTGC | 1 cut(s) | 869 |
| BspPI | GGATC | 2 cut(s) | 44, 741 |
| BspTNI | GGTCTC | 1 cut(s) | 1069 |
| BsrBI | CCGCTC | 1 cut(s) | 1096 |
| BsrDI | GCAATG | 2 cut(s) | 49, 999 |
| BsrGI | TGTACA | 3 cut(s) | 235, 401, 1000 |
| BsrI | ACTGG | 3 cut(s) | 382, 461, 520 |
| BssECI | CCNNGG | 1 cut(s) | 31 |
| BssMI | GATC | 4 cut(s) | 36, 120, 388, 746 |
| Bst4CI | ACNGT | 2 cut(s) | 247, 978 |
| Bst6I | CTCTTC | 1 cut(s) | 578 |
| BstAUI | TGTACA | 3 cut(s) | 235, 401, 1000 |
| BstC8I | GCNNGC | 3 cut(s) | 432, 495, 627 |
| BstEII | GGTNACC | 1 cut(s) | 317 |
| BstF5I | GGATG | 2 cut(s) | 88, 1087 |
| BstH2I | RGCGCY | 1 cut(s) | 65 |
| BstHHI | GCGC | 2 cut(s) | 64, 265 |
| BstKTI | GATC | 4 cut(s) | 39, 123, 391, 749 |
| BstMAI | GTCTC | 3 cut(s) | 107, 1069, 1095 |
| BstMBI | GATC | 4 cut(s) | 36, 120, 388, 746 |
| BstNSI | RCATGY | 1 cut(s) | 434 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 879 |
| BstPI | GGTNACC | 1 cut(s) | 317 |
| BstV1I | GCAGC | 3 cut(s) | 548, 616, 793 |
| BstX2I | RGATCY | 1 cut(s) | 36 |
| BstYI | RGATCY | 1 cut(s) | 36 |
| BsuI | GTATCC | 2 cut(s) | 555, 1068 |
| BtgZI | GCGATG | 1 cut(s) | 230 |
| BtrI | CACGTC | 1 cut(s) | 511 |
| BtsCI | GGATG | 2 cut(s) | 88, 1087 |
| BtsI | GCAGTG | 1 cut(s) | 954 |
| BtsIMutI | CAGTG | 1 cut(s) | 954 |
| BveI | ACCTGC | 1 cut(s) | 869 |
| Cac8I | GCNNGC | 3 cut(s) | 432, 495, 627 |
| CfoI | GCGC | 2 cut(s) | 64, 265 |
| Cfr13I | GGNCC | 1 cut(s) | 1038 |
| CseI | GACGC | 1 cut(s) | 884 |
| Csp6I | GTAC | 3 cut(s) | 236, 402, 1001 |
| CviAII | CATG | 5 cut(s) | 357, 392, 431, 498, 759 |
| CviQI | GTAC | 3 cut(s) | 236, 402, 1001 |
| DpnI | GATC | 4 cut(s) | 38, 122, 390, 748 |
| DpnII | GATC | 4 cut(s) | 36, 120, 388, 746 |
| Eam1104I | CTCTTC | 1 cut(s) | 578 |
| EarI | CTCTTC | 1 cut(s) | 578 |
| Eco31I | GGTCTC | 1 cut(s) | 1069 |
| Eco32I | GATATC | 1 cut(s) | 421 |
| Eco47I | GGWCC | 1 cut(s) | 1038 |
| Eco57I | CTGAAG | 2 cut(s) | 86, 1161 |
| Eco88I | CYCGRG | 1 cut(s) | 1072 |
| Eco91I | GGTNACC | 1 cut(s) | 317 |
| EcoO65I | GGTNACC | 1 cut(s) | 317 |
| EcoRI | GAATTC | 1 cut(s) | 364 |
| EcoRV | GATATC | 1 cut(s) | 421 |
| Esp3I | CGTCTC | 1 cut(s) | 107 |
| FaeI | CATG | 5 cut(s) | 360, 395, 434, 501, 762 |
| FaqI | GGGAC | 1 cut(s) | 1130 |
| FatI | CATG | 5 cut(s) | 356, 391, 430, 497, 758 |
| FblI | GTMKAC | 1 cut(s) | 353 |
| Fnu4HI | GCNGC | 3 cut(s) | 537, 605, 807 |
| FokI | GGATG | 2 cut(s) | 75, 1094 |
| Fsp4HI | GCNGC | 3 cut(s) | 537, 605, 807 |
| FspBI | CTAG | 2 cut(s) | 600, 647 |
| FspI | TGCGCA | 1 cut(s) | 264 |
| GlaI | GCGC | 2 cut(s) | 63, 264 |
| GluI | GCNGC | 3 cut(s) | 537, 605, 807 |
| GsuI | CTGGAG | 1 cut(s) | 365 |
| HaeII | RGCGCY | 1 cut(s) | 65 |
| HapII | CCGG | 1 cut(s) | 1079 |
| HgaI | GACGC | 1 cut(s) | 884 |
| HhaI | GCGC | 2 cut(s) | 64, 265 |
| Hin1II | CATG | 5 cut(s) | 360, 395, 434, 501, 762 |
| Hin6I | GCGC | 2 cut(s) | 62, 263 |
| HinP1I | GCGC | 2 cut(s) | 62, 263 |
| HincII | GTYRAC | 1 cut(s) | 1173 |
| HindII | GTYRAC | 1 cut(s) | 1173 |
| HindIII | AAGCTT | 1 cut(s) | 787 |
| HinfI | GANTC | 4 cut(s) | 85, 438, 709, 728 |
| HpaII | CCGG | 1 cut(s) | 1079 |
| HphI | GGTGA | 2 cut(s) | 329, 1045 |
| Hpy166II | GTNNAC | 6 cut(s) | 354, 404, 555, 921, 952, 1173 |
| Hpy188I | TCNGA | 1 cut(s) | 370 |
| Hpy188III | TCNNGA | 8 cut(s) | 204, 223, 326, 595, 635, 736, 884, 1182 |
| Hpy8I | GTNNAC | 6 cut(s) | 354, 404, 555, 921, 952, 1173 |
| HpyAV | CCTTC | 3 cut(s) | 61, 332, 760 |
| HpyCH4III | ACNGT | 2 cut(s) | 247, 978 |
| HpyCH4IV | ACGT | 1 cut(s) | 510 |
| HpyCH4V | TGCA | 8 cut(s) | 42, 151, 395, 434, 536, 673, 706, 1130 |
| HpySE526I | ACGT | 1 cut(s) | 510 |
| Hsp92II | CATG | 5 cut(s) | 360, 395, 434, 501, 762 |
| HspAI | GCGC | 2 cut(s) | 62, 263 |
| Kzo9I | GATC | 4 cut(s) | 36, 120, 388, 746 |
| Lsp1109I | GCAGC | 3 cut(s) | 548, 616, 793 |
| LweI | GCATC | 2 cut(s) | 160, 274 |
| MaeI | CTAG | 2 cut(s) | 600, 647 |
| MaeII | ACGT | 1 cut(s) | 510 |
| MaeIII | GTNAC | 5 cut(s) | 317, 511, 642, 754, 982 |
| MalI | GATC | 4 cut(s) | 38, 122, 390, 748 |
| MbiI | CCGCTC | 1 cut(s) | 1096 |
| MboI | GATC | 4 cut(s) | 36, 120, 388, 746 |
| MboII | GAAGA | 6 cut(s) | 130, 556, 595, 704, 737, 1170 |
| MfeI | CAATTG | 1 cut(s) | 1004 |
| MflI | RGATCY | 1 cut(s) | 36 |
| MhlI | GDGCHC | 1 cut(s) | 631 |
| MluCI | AATT | 6 cut(s) | 25, 364, 443, 701, 859, 1004 |
| MmeI | TCCRAC | 2 cut(s) | 142, 1129 |
| MroXI | GAANNNNTTC | 2 cut(s) | 827, 1146 |
| MspI | CCGG | 1 cut(s) | 1079 |
| MunI | CAATTG | 1 cut(s) | 1004 |
| Mva1269I | GAATGC | 1 cut(s) | 172 |
| NdeII | GATC | 4 cut(s) | 36, 120, 388, 746 |
| NlaIII | CATG | 5 cut(s) | 360, 395, 434, 501, 762 |
| NmeAIII | GCCGAG | 1 cut(s) | 56 |
| NmuCI | GTSAC | 3 cut(s) | 317, 511, 754 |
| NsbI | TGCGCA | 1 cut(s) | 264 |
| NspI | RCATGY | 1 cut(s) | 434 |
| PaeI | GCATGC | 1 cut(s) | 434 |
| PctI | GAATGC | 1 cut(s) | 172 |
| PdmI | GAANNNNTTC | 2 cut(s) | 827, 1146 |
| PfeI | GAWTC | 4 cut(s) | 85, 438, 709, 728 |
| PkrI | GCNGC | 3 cut(s) | 538, 606, 808 |
| PshAI | GACNNNNGTC | 1 cut(s) | 879 |
| PspEI | GGTNACC | 1 cut(s) | 317 |
| PspPI | GGNCC | 1 cut(s) | 1038 |
| PsuI | RGATCY | 1 cut(s) | 36 |
| RsaI | GTAC | 3 cut(s) | 237, 403, 1002 |
| RsaNI | GTAC | 3 cut(s) | 236, 402, 1001 |
| SatI | GCNGC | 3 cut(s) | 537, 605, 807 |
| Sau3AI | GATC | 4 cut(s) | 36, 120, 388, 746 |
| Sau96I | GGNCC | 1 cut(s) | 1038 |
| SduI | GDGCHC | 1 cut(s) | 631 |
| SfaNI | GCATC | 2 cut(s) | 160, 274 |
| SinI | GGWCC | 1 cut(s) | 1038 |
| SmlI | CTYRAG | 1 cut(s) | 143 |
| SmoI | CTYRAG | 1 cut(s) | 143 |
| SphI | GCATGC | 1 cut(s) | 434 |
| Sse9I | AATT | 6 cut(s) | 25, 364, 443, 701, 859, 1004 |
| SsiI | CCGC | 3 cut(s) | 170, 1041, 1094 |
| SspMI | CTAG | 2 cut(s) | 600, 647 |
| TaaI | ACNGT | 2 cut(s) | 247, 978 |
| TaiI | ACGT | 1 cut(s) | 513 |
| TaqI | TCGA | 2 cut(s) | 273, 799 |
| TasI | AATT | 6 cut(s) | 25, 364, 443, 701, 859, 1004 |
| TatI | WGTACW | 3 cut(s) | 235, 401, 1000 |
| TfiI | GAWTC | 4 cut(s) | 85, 438, 709, 728 |
| TscAI | CASTG | 1 cut(s) | 954 |
| TseFI | GTSAC | 3 cut(s) | 317, 511, 754 |
| TseI | GCWGC | 3 cut(s) | 536, 604, 806 |
| Tsp45I | GTSAC | 3 cut(s) | 317, 511, 754 |
| TspDTI | ATGAA | 6 cut(s) | 693, 785, 820, 864, 1010, 1139 |
| TspRI | CASTG | 1 cut(s) | 954 |
| VpaK11BI | GGWCC | 1 cut(s) | 1038 |
| XapI | RAATTY | 2 cut(s) | 364, 443 |
| XceI | RCATGY | 1 cut(s) | 434 |
| XmiI | GTMKAC | 1 cut(s) | 353 |
| XmnI | GAANNNNTTC | 2 cut(s) | 827, 1146 |
| XspI | CTAG | 2 cut(s) | 600, 647 |