ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 171, 264 |
| AccBSI | CCGCTC | 1 cut(s) | 593 |
| AccII | CGCG | 1 cut(s) | 545 |
| AciI | CCGC | 3 cut(s) | 469, 593, 650 |
| AclWI | GGATC | 1 cut(s) | 764 |
| AcsI | RAATTY | 1 cut(s) | 734 |
| AcuI | CTGAAG | 1 cut(s) | 868 |
| AcyI | GRCGYC | 1 cut(s) | 359 |
| AfaI | GTAC | 2 cut(s) | 253, 639 |
| AfeI | AGCGCT | 1 cut(s) | 147 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 405, 579, 781 |
| AflIII | ACRYGT | 1 cut(s) | 244 |
| AgsI | TTSAA | 6 cut(s) | 142, 427, 439, 539, 633, 800 |
| AjnI | CCWGG | 2 cut(s) | 295, 866 |
| AluBI | AGCT | 3 cut(s) | 62, 503, 693 |
| AluI | AGCT | 3 cut(s) | 62, 503, 693 |
| Alw21I | GWGCWC | 1 cut(s) | 814 |
| Alw26I | GTCTC | 3 cut(s) | 107, 307, 510 |
| AlwI | GGATC | 1 cut(s) | 764 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 296 |
| Aor51HI | AGCGCT | 1 cut(s) | 147 |
| AoxI | GGCC | 1 cut(s) | 461 |
| ApeKI | GCWGC | 2 cut(s) | 148, 754 |
| ApoI | RAATTY | 1 cut(s) | 734 |
| AseI | ATTAAT | 1 cut(s) | 843 |
| AspLEI | GCGC | 6 cut(s) | 130, 148, 172, 265, 545, 859 |
| AspS9I | GGNCC | 2 cut(s) | 293, 461 |
| AsuHPI | GGTGA | 2 cut(s) | 311, 359 |
| AvaII | GGWCC | 1 cut(s) | 293 |
| Bbv12I | GWGCWC | 1 cut(s) | 814 |
| BbvI | GCAGC | 2 cut(s) | 135, 766 |
| BciT130I | CCWGG | 2 cut(s) | 297, 868 |
| BclI | TGATCA | 1 cut(s) | 75 |
| BcoDI | GTCTC | 3 cut(s) | 107, 307, 510 |
| BfaI | CTAG | 4 cut(s) | 57, 521, 530, 860 |
| BfoI | RGCGCY | 1 cut(s) | 149 |
| BisI | GCNGC | 2 cut(s) | 149, 755 |
| BlsI | GCNGC | 2 cut(s) | 150, 756 |
| Bme1390I | CCNGG | 2 cut(s) | 297, 868 |
| Bme18I | GGWCC | 1 cut(s) | 293 |
| BmgT120I | GGNCC | 2 cut(s) | 293, 461 |
| BmrFI | CCNGG | 2 cut(s) | 297, 868 |
| BmrI | ACTGGG | 1 cut(s) | 648 |
| BmsI | GCATC | 3 cut(s) | 274, 351, 766 |
| BmuI | ACTGGG | 1 cut(s) | 648 |
| BpmI | CTGGAG | 1 cut(s) | 225 |
| Bpu10I | CCTNAGC | 1 cut(s) | 131 |
| Bsa29I | ATCGAT | 1 cut(s) | 727 |
| BsaAI | YACGTR | 1 cut(s) | 377 |
| BsaBI | GATNNNNATC | 3 cut(s) | 237, 413, 663 |
| BsaHI | GRCGYC | 1 cut(s) | 359 |
| BsaI | GGTCTC | 1 cut(s) | 510 |
| BsaJI | CCNNGG | 2 cut(s) | 305, 404 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 450, 480, 507, 537 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 405, 579, 781 |
| Bse1I | ACTGG | 1 cut(s) | 654 |
| Bse8I | GATNNNNATC | 3 cut(s) | 237, 413, 663 |
| BseBI | CCWGG | 2 cut(s) | 297, 868 |
| BseCI | ATCGAT | 1 cut(s) | 727 |
| BseDI | CCNNGG | 2 cut(s) | 305, 404 |
| BseJI | GATNNNNATC | 3 cut(s) | 237, 413, 663 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 405, 579, 781 |
| BseMII | CTCAG | 1 cut(s) | 179 |
| BseNI | ACTGG | 1 cut(s) | 654 |
| BseXI | GCAGC | 2 cut(s) | 135, 766 |
| Bsh1236I | CGCG | 1 cut(s) | 545 |
| Bsh1285I | CGRYCG | 1 cut(s) | 473 |
| BshFI | GGCC | 1 cut(s) | 463 |
| BshVI | ATCGAT | 1 cut(s) | 727 |
| BsiEI | CGRYCG | 1 cut(s) | 473 |
| BsiHKAI | GWGCWC | 1 cut(s) | 814 |
| BslFI | GGGAC | 1 cut(s) | 613 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 405, 579, 781 |
| BsmAI | GTCTC | 3 cut(s) | 107, 307, 510 |
| BsmFI | GGGAC | 1 cut(s) | 613 |
| BsmI | GAATGC | 1 cut(s) | 568 |
| BsnI | GGCC | 1 cut(s) | 463 |
| Bso31I | GGTCTC | 1 cut(s) | 510 |
| Bsp1286I | GDGCHC | 1 cut(s) | 814 |
| Bsp143I | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| Bsp19I | CCATGG | 1 cut(s) | 404 |
| BspACI | CCGC | 3 cut(s) | 469, 593, 650 |
| BspANI | GGCC | 1 cut(s) | 463 |
| BspCNI | CTCAG | 1 cut(s) | 180 |
| BspDI | ATCGAT | 1 cut(s) | 727 |
| BspFNI | CGCG | 1 cut(s) | 545 |
| BspPI | GGATC | 1 cut(s) | 764 |
| BspTNI | GGTCTC | 1 cut(s) | 510 |
| BsrBI | CCGCTC | 1 cut(s) | 593 |
| BsrI | ACTGG | 1 cut(s) | 654 |
| BssECI | CCNNGG | 2 cut(s) | 305, 404 |
| BssMI | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| BssNI | GRCGYC | 1 cut(s) | 359 |
| BssT1I | CCWWGG | 1 cut(s) | 404 |
| Bst2UI | CCWGG | 2 cut(s) | 297, 868 |
| Bst4CI | ACNGT | 3 cut(s) | 306, 332, 582 |
| BstACI | GRCGYC | 1 cut(s) | 359 |
| BstBAI | YACGTR | 1 cut(s) | 377 |
| BstC8I | GCNNGC | 4 cut(s) | 201, 340, 687, 691 |
| BstDEI | CTNAG | 3 cut(s) | 131, 177, 188 |
| BstDSI | CCRYGG | 2 cut(s) | 305, 404 |
| BstFNI | CGCG | 1 cut(s) | 545 |
| BstH2I | RGCGCY | 1 cut(s) | 149 |
| BstHHI | GCGC | 6 cut(s) | 130, 148, 172, 265, 545, 859 |
| BstKTI | GATC | 5 cut(s) | 78, 241, 529, 667, 772 |
| BstMAI | GTCTC | 3 cut(s) | 107, 307, 510 |
| BstMBI | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| BstMCI | CGRYCG | 1 cut(s) | 473 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 718 |
| BstNI | CCWGG | 2 cut(s) | 297, 868 |
| BstNSI | RCATGY | 1 cut(s) | 248 |
| BstSCI | CCNGG | 2 cut(s) | 295, 866 |
| BstUI | CGCG | 1 cut(s) | 545 |
| BstV1I | GCAGC | 2 cut(s) | 135, 766 |
| Bsu15I | ATCGAT | 1 cut(s) | 727 |
| BsuRI | GGCC | 1 cut(s) | 463 |
| BsuTUI | ATCGAT | 1 cut(s) | 727 |
| BtgI | CCRYGG | 2 cut(s) | 305, 404 |
| BtsIMutI | CAGTG | 2 cut(s) | 130, 661 |
| Cac8I | GCNNGC | 4 cut(s) | 201, 340, 687, 691 |
| CaiI | CAGNNNCTG | 1 cut(s) | 296 |
| CfoI | GCGC | 6 cut(s) | 130, 148, 172, 265, 545, 859 |
| Cfr13I | GGNCC | 2 cut(s) | 293, 461 |
| ClaI | ATCGAT | 1 cut(s) | 727 |
| CseI | GACGC | 2 cut(s) | 348, 701 |
| CsiI | ACCWGGT | 1 cut(s) | 295 |
| Csp6I | GTAC | 2 cut(s) | 252, 638 |
| CviAII | CATG | 5 cut(s) | 245, 405, 555, 609, 823 |
| CviJI | RGCY | 9 cut(s) | 62, 91, 203, 386, 463, 492, 503, 693, 785 |
| CviKI_1 | RGCY | 9 cut(s) | 62, 91, 203, 386, 463, 492, 503, 693, 785 |
| CviQI | GTAC | 2 cut(s) | 252, 638 |
| DdeI | CTNAG | 3 cut(s) | 131, 177, 188 |
| DpnI | GATC | 5 cut(s) | 77, 240, 528, 666, 771 |
| DpnII | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| DraI | TTTAAA | 1 cut(s) | 733 |
| Eco130I | CCWWGG | 1 cut(s) | 404 |
| Eco31I | GGTCTC | 1 cut(s) | 510 |
| Eco47I | GGWCC | 1 cut(s) | 293 |
| Eco47III | AGCGCT | 1 cut(s) | 147 |
| Eco57I | CTGAAG | 1 cut(s) | 868 |
| EcoO109I | RGGNCCY | 2 cut(s) | 293, 461 |
| EcoRII | CCWGG | 2 cut(s) | 295, 866 |
| EcoT14I | CCWWGG | 1 cut(s) | 404 |
| ErhI | CCWWGG | 1 cut(s) | 404 |
| FaeI | CATG | 5 cut(s) | 248, 408, 558, 612, 826 |
| FaqI | GGGAC | 1 cut(s) | 613 |
| FatI | CATG | 5 cut(s) | 244, 404, 554, 608, 822 |
| FbaI | TGATCA | 1 cut(s) | 75 |
| Fnu4HI | GCNGC | 2 cut(s) | 149, 755 |
| Fsp4HI | GCNGC | 2 cut(s) | 149, 755 |
| FspBI | CTAG | 4 cut(s) | 57, 521, 530, 860 |
| FspI | TGCGCA | 2 cut(s) | 171, 264 |
| GlaI | GCGC | 6 cut(s) | 129, 147, 171, 264, 544, 858 |
| GluI | GCNGC | 2 cut(s) | 149, 755 |
| GsuI | CTGGAG | 1 cut(s) | 225 |
| HaeII | RGCGCY | 1 cut(s) | 149 |
| HaeIII | GGCC | 1 cut(s) | 463 |
| HgaI | GACGC | 2 cut(s) | 348, 701 |
| HhaI | GCGC | 6 cut(s) | 130, 148, 172, 265, 545, 859 |
| Hin1I | GRCGYC | 1 cut(s) | 359 |
| Hin1II | CATG | 5 cut(s) | 248, 408, 558, 612, 826 |
| Hin6I | GCGC | 6 cut(s) | 128, 146, 170, 263, 543, 857 |
| HinP1I | GCGC | 6 cut(s) | 128, 146, 170, 263, 543, 857 |
| HincII | GTYRAC | 1 cut(s) | 828 |
| HindII | GTYRAC | 1 cut(s) | 828 |
| HindIII | AAGCTT | 1 cut(s) | 501 |
| HinfI | GANTC | 4 cut(s) | 36, 395, 659, 740 |
| HpaI | GTTAAC | 1 cut(s) | 828 |
| HphI | GGTGA | 2 cut(s) | 311, 359 |
| Hpy166II | GTNNAC | 3 cut(s) | 302, 350, 828 |
| Hpy188I | TCNGA | 4 cut(s) | 48, 229, 475, 664 |
| Hpy8I | GTNNAC | 3 cut(s) | 302, 350, 828 |
| HpyAV | CCTTC | 2 cut(s) | 410, 787 |
| HpyCH4III | ACNGT | 3 cut(s) | 306, 332, 582 |
| HpyCH4IV | ACGT | 3 cut(s) | 352, 376, 874 |
| HpyCH4V | TGCA | 6 cut(s) | 30, 151, 338, 566, 685, 754 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 718 |
| HpyF3I | CTNAG | 3 cut(s) | 131, 177, 188 |
| HpySE526I | ACGT | 3 cut(s) | 352, 376, 874 |
| Hsp92I | GRCGYC | 1 cut(s) | 359 |
| Hsp92II | CATG | 5 cut(s) | 248, 408, 558, 612, 826 |
| HspAI | GCGC | 6 cut(s) | 128, 146, 170, 263, 543, 857 |
| Ksp22I | TGATCA | 1 cut(s) | 75 |
| KspAI | GTTAAC | 1 cut(s) | 828 |
| Kzo9I | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| LmnI | GCTCC | 1 cut(s) | 817 |
| Lsp1109I | GCAGC | 2 cut(s) | 135, 766 |
| LweI | GCATC | 3 cut(s) | 274, 351, 766 |
| MabI | ACCWGGT | 1 cut(s) | 295 |
| MaeI | CTAG | 4 cut(s) | 57, 521, 530, 860 |
| MaeII | ACGT | 3 cut(s) | 352, 376, 874 |
| MalI | GATC | 5 cut(s) | 77, 240, 528, 666, 771 |
| MbiI | CCGCTC | 1 cut(s) | 593 |
| MboI | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| MboII | GAAGA | 3 cut(s) | 242, 559, 812 |
| MhlI | GDGCHC | 1 cut(s) | 814 |
| MluCI | AATT | 6 cut(s) | 25, 158, 575, 734, 787, 837 |
| MlyI | GAGTC | 2 cut(s) | 389, 749 |
| MmeI | TCCRAC | 3 cut(s) | 71, 453, 564 |
| MnlI | CCTC | 6 cut(s) | 60, 102, 200, 452, 482, 800 |
| MseI | TTAA | 3 cut(s) | 732, 827, 843 |
| MspR9I | CCNGG | 2 cut(s) | 297, 868 |
| Mva1269I | GAATGC | 1 cut(s) | 568 |
| MvaI | CCWGG | 2 cut(s) | 297, 868 |
| MvnI | CGCG | 1 cut(s) | 545 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 718 |
| NcoI | CCATGG | 1 cut(s) | 404 |
| NdeII | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| NlaIII | CATG | 5 cut(s) | 248, 408, 558, 612, 826 |
| NsbI | TGCGCA | 2 cut(s) | 171, 264 |
| NspI | RCATGY | 1 cut(s) | 248 |
| PciI | ACATGT | 1 cut(s) | 244 |
| PctI | GAATGC | 1 cut(s) | 568 |
| PfeI | GAWTC | 2 cut(s) | 36, 659 |
| PkrI | GCNGC | 2 cut(s) | 150, 756 |
| PleI | GAGTC | 2 cut(s) | 389, 748 |
| PpsI | GAGTC | 2 cut(s) | 389, 748 |
| Ppu21I | YACGTR | 1 cut(s) | 377 |
| PpuMI | RGGWCCY | 1 cut(s) | 293 |
| PscI | ACATGT | 1 cut(s) | 244 |
| PshBI | ATTAAT | 1 cut(s) | 843 |
| Psp5II | RGGWCCY | 1 cut(s) | 293 |
| Psp6I | CCWGG | 2 cut(s) | 295, 866 |
| PspGI | CCWGG | 2 cut(s) | 295, 866 |
| PspPI | GGNCC | 2 cut(s) | 293, 461 |
| PspPPI | RGGWCCY | 1 cut(s) | 293 |
| PstNI | CAGNNNCTG | 1 cut(s) | 296 |
| RsaI | GTAC | 2 cut(s) | 253, 639 |
| RsaNI | GTAC | 2 cut(s) | 252, 638 |
| SaqAI | TTAA | 3 cut(s) | 732, 827, 843 |
| SatI | GCNGC | 2 cut(s) | 149, 755 |
| Sau3AI | GATC | 5 cut(s) | 75, 238, 526, 664, 769 |
| Sau96I | GGNCC | 2 cut(s) | 293, 461 |
| SchI | GAGTC | 2 cut(s) | 389, 749 |
| ScrFI | CCNGG | 2 cut(s) | 297, 868 |
| SduI | GDGCHC | 1 cut(s) | 814 |
| SexAI | ACCWGGT | 1 cut(s) | 295 |
| SfaNI | GCATC | 3 cut(s) | 274, 351, 766 |
| SinI | GGWCC | 1 cut(s) | 293 |
| SmiI | ATTTAAAT | 1 cut(s) | 733 |
| Sse9I | AATT | 6 cut(s) | 25, 158, 575, 734, 787, 837 |
| SsiI | CCGC | 3 cut(s) | 469, 593, 650 |
| SspMI | CTAG | 4 cut(s) | 57, 521, 530, 860 |
| StyD4I | CCNGG | 2 cut(s) | 295, 866 |
| StyI | CCWWGG | 1 cut(s) | 404 |
| SwaI | ATTTAAAT | 1 cut(s) | 733 |
| TaaI | ACNGT | 3 cut(s) | 306, 332, 582 |
| TaiI | ACGT | 3 cut(s) | 355, 379, 877 |
| TaqI | TCGA | 1 cut(s) | 727 |
| TasI | AATT | 6 cut(s) | 25, 158, 575, 734, 787, 837 |
| TfiI | GAWTC | 2 cut(s) | 36, 659 |
| Tru1I | TTAA | 3 cut(s) | 732, 827, 843 |
| Tru9I | TTAA | 3 cut(s) | 732, 827, 843 |
| TscAI | CASTG | 2 cut(s) | 130, 661 |
| TseI | GCWGC | 2 cut(s) | 148, 754 |
| TspDTI | ATGAA | 2 cut(s) | 17, 811 |
| TspGWI | ACGGA | 2 cut(s) | 239, 485 |
| TspRI | CASTG | 2 cut(s) | 130, 661 |
| VpaK11BI | GGWCC | 1 cut(s) | 293 |
| VspI | ATTAAT | 1 cut(s) | 843 |
| XapI | RAATTY | 1 cut(s) | 734 |
| XceI | RCATGY | 1 cut(s) | 248 |
| XspI | CTAG | 4 cut(s) | 57, 521, 530, 860 |