ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 68, 179 |
| Acc65I | GGTACC | 1 cut(s) | 222 |
| AccB1I | GGYRCC | 1 cut(s) | 222 |
| AccI | GTMKAC | 1 cut(s) | 171 |
| AcoI | YGGCCR | 2 cut(s) | 3, 211 |
| AcuI | CTGAAG | 3 cut(s) | 435, 525, 650 |
| AcyI | GRCGYC | 2 cut(s) | 69, 141 |
| AfaI | GTAC | 3 cut(s) | 11, 224, 278 |
| AfeI | AGCGCT | 1 cut(s) | 241 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 43, 198, 199, 590 |
| AgsI | TTSAA | 2 cut(s) | 43, 115 |
| AjnI | CCWGG | 1 cut(s) | 92 |
| AluBI | AGCT | 3 cut(s) | 257, 404, 654 |
| AluI | AGCT | 3 cut(s) | 257, 404, 654 |
| Alw21I | GWGCWC | 1 cut(s) | 268 |
| Alw26I | GTCTC | 1 cut(s) | 412 |
| Alw44I | GTGCAC | 1 cut(s) | 264 |
| Ama87I | CYCGRG | 1 cut(s) | 119 |
| Aor51HI | AGCGCT | 1 cut(s) | 241 |
| AoxI | GGCC | 3 cut(s) | 3, 189, 211 |
| ApaLI | GTGCAC | 1 cut(s) | 264 |
| ApeKI | GCWGC | 1 cut(s) | 645 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 110, 571 |
| Asp718I | GGTACC | 1 cut(s) | 222 |
| AspLEI | GCGC | 1 cut(s) | 242 |
| AspS9I | GGNCC | 1 cut(s) | 190 |
| AsuC2I | CCSGG | 1 cut(s) | 7 |
| AvaI | CYCGRG | 1 cut(s) | 119 |
| BaeGI | GKGCMC | 1 cut(s) | 268 |
| BalI | TGGCCA | 1 cut(s) | 213 |
| BanI | GGYRCC | 1 cut(s) | 222 |
| Bbv12I | GWGCWC | 1 cut(s) | 268 |
| BbvI | GCAGC | 1 cut(s) | 632 |
| BccI | CCATC | 2 cut(s) | 312, 413 |
| BceAI | ACGGC | 1 cut(s) | 585 |
| BciT130I | CCWGG | 1 cut(s) | 94 |
| BcnI | CCSGG | 1 cut(s) | 7 |
| BcoDI | GTCTC | 1 cut(s) | 412 |
| BfaI | CTAG | 1 cut(s) | 655 |
| BfoI | RGCGCY | 1 cut(s) | 243 |
| BglII | AGATCT | 1 cut(s) | 385 |
| BisI | GCNGC | 1 cut(s) | 646 |
| BlsI | GCNGC | 1 cut(s) | 647 |
| Bme1390I | CCNGG | 2 cut(s) | 7, 94 |
| BmeT110I | CYCGRG | 1 cut(s) | 119 |
| BmgT120I | GGNCC | 1 cut(s) | 190 |
| BmiI | GGNNCC | 2 cut(s) | 224, 338 |
| BmrFI | CCNGG | 2 cut(s) | 7, 94 |
| BmsI | GCATC | 1 cut(s) | 354 |
| BpmI | CTGGAG | 1 cut(s) | 76 |
| BpuEI | CTTGAG | 1 cut(s) | 634 |
| BpuMI | CCSGG | 1 cut(s) | 7 |
| BsaHI | GRCGYC | 2 cut(s) | 69, 141 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 43, 198, 199, 590 |
| Bse1I | ACTGG | 1 cut(s) | 477 |
| BseBI | CCWGG | 1 cut(s) | 94 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 43, 198, 199, 590 |
| BseMII | CTCAG | 1 cut(s) | 426 |
| BseNI | ACTGG | 1 cut(s) | 477 |
| BseSI | GKGCMC | 1 cut(s) | 268 |
| BseXI | GCAGC | 1 cut(s) | 632 |
| BseYI | CCCAGC | 1 cut(s) | 583 |
| BsgI | GTGCAG | 1 cut(s) | 453 |
| BshFI | GGCC | 3 cut(s) | 5, 191, 213 |
| BshNI | GGYRCC | 1 cut(s) | 222 |
| BsiHKAI | GWGCWC | 1 cut(s) | 268 |
| BsiHKCI | CYCGRG | 1 cut(s) | 119 |
| BsiSI | CCGG | 2 cut(s) | 6, 66 |
| BslFI | GGGAC | 2 cut(s) | 215, 566 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 43, 198, 199, 590 |
| BsmAI | GTCTC | 1 cut(s) | 412 |
| BsmFI | GGGAC | 2 cut(s) | 215, 566 |
| BsnI | GGCC | 3 cut(s) | 5, 191, 213 |
| BsoBI | CYCGRG | 1 cut(s) | 119 |
| Bsp1286I | GDGCHC | 1 cut(s) | 268 |
| Bsp143I | GATC | 2 cut(s) | 54, 385 |
| BspANI | GGCC | 3 cut(s) | 5, 191, 213 |
| BspCNI | CTCAG | 1 cut(s) | 427 |
| BspLI | GGNNCC | 2 cut(s) | 224, 338 |
| BspT107I | GGYRCC | 1 cut(s) | 222 |
| BsrI | ACTGG | 1 cut(s) | 477 |
| BssMI | GATC | 2 cut(s) | 54, 385 |
| BssNI | GRCGYC | 2 cut(s) | 69, 141 |
| Bst2UI | CCWGG | 1 cut(s) | 94 |
| Bst4CI | ACNGT | 1 cut(s) | 227 |
| Bst6I | CTCTTC | 2 cut(s) | 44, 626 |
| BstACI | GRCGYC | 2 cut(s) | 69, 141 |
| BstC8I | GCNNGC | 1 cut(s) | 211 |
| BstDEI | CTNAG | 1 cut(s) | 435 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 41 |
| BstH2I | RGCGCY | 1 cut(s) | 243 |
| BstHHI | GCGC | 1 cut(s) | 242 |
| BstKTI | GATC | 2 cut(s) | 57, 388 |
| BstMAI | GTCTC | 1 cut(s) | 412 |
| BstMBI | GATC | 2 cut(s) | 54, 385 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 66, 263, 476, 651 |
| BstNI | CCWGG | 1 cut(s) | 94 |
| BstSCI | CCNGG | 2 cut(s) | 5, 92 |
| BstSLI | GKGCMC | 1 cut(s) | 268 |
| BstV1I | GCAGC | 1 cut(s) | 632 |
| BstX2I | RGATCY | 1 cut(s) | 385 |
| BstYI | RGATCY | 1 cut(s) | 385 |
| BsuRI | GGCC | 3 cut(s) | 5, 191, 213 |
| BtsI | GCAGTG | 1 cut(s) | 204 |
| BtsIMutI | CAGTG | 3 cut(s) | 204, 342, 470 |
| Cac8I | GCNNGC | 1 cut(s) | 211 |
| CfoI | GCGC | 1 cut(s) | 242 |
| Cfr13I | GGNCC | 1 cut(s) | 190 |
| CseI | GACGC | 2 cut(s) | 58, 130 |
| Csp6I | GTAC | 3 cut(s) | 10, 223, 277 |
| CviAII | CATG | 2 cut(s) | 293, 457 |
| CviQI | GTAC | 3 cut(s) | 10, 223, 277 |
| DdeI | CTNAG | 1 cut(s) | 435 |
| DpnI | GATC | 2 cut(s) | 56, 387 |
| DpnII | GATC | 2 cut(s) | 54, 385 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 68, 179 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 68, 179 |
| EaeI | YGGCCR | 2 cut(s) | 3, 211 |
| Eam1104I | CTCTTC | 2 cut(s) | 44, 626 |
| EarI | CTCTTC | 2 cut(s) | 44, 626 |
| Eco47III | AGCGCT | 1 cut(s) | 241 |
| Eco57I | CTGAAG | 3 cut(s) | 435, 525, 650 |
| Eco88I | CYCGRG | 1 cut(s) | 119 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 41 |
| EcoRII | CCWGG | 1 cut(s) | 92 |
| FaeI | CATG | 2 cut(s) | 296, 460 |
| FaiI | YATR | 5 cut(s) | 294, 432, 458, 528, 608 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 35, 67 |
| FaqI | GGGAC | 2 cut(s) | 215, 566 |
| FatI | CATG | 2 cut(s) | 292, 456 |
| FblI | GTMKAC | 1 cut(s) | 171 |
| Fnu4HI | GCNGC | 1 cut(s) | 646 |
| Fsp4HI | GCNGC | 1 cut(s) | 646 |
| FspBI | CTAG | 1 cut(s) | 655 |
| GlaI | GCGC | 1 cut(s) | 241 |
| GluI | GCNGC | 1 cut(s) | 646 |
| GsaI | CCCAGC | 1 cut(s) | 587 |
| GsuI | CTGGAG | 1 cut(s) | 76 |
| HaeII | RGCGCY | 1 cut(s) | 243 |
| HaeIII | GGCC | 3 cut(s) | 5, 191, 213 |
| HapII | CCGG | 2 cut(s) | 6, 66 |
| HgaI | GACGC | 2 cut(s) | 58, 130 |
| HhaI | GCGC | 1 cut(s) | 242 |
| Hin1I | GRCGYC | 2 cut(s) | 69, 141 |
| Hin1II | CATG | 2 cut(s) | 296, 460 |
| Hin6I | GCGC | 1 cut(s) | 240 |
| HinP1I | GCGC | 1 cut(s) | 240 |
| HincII | GTYRAC | 2 cut(s) | 172, 313 |
| HindII | GTYRAC | 2 cut(s) | 172, 313 |
| HinfI | GANTC | 5 cut(s) | 62, 122, 347, 443, 514 |
| HpaII | CCGG | 2 cut(s) | 6, 66 |
| Hpy166II | GTNNAC | 6 cut(s) | 99, 132, 172, 266, 277, 313 |
| Hpy188I | TCNGA | 2 cut(s) | 385, 630 |
| Hpy188III | TCNNGA | 2 cut(s) | 52, 511 |
| Hpy8I | GTNNAC | 6 cut(s) | 99, 132, 172, 266, 277, 313 |
| Hpy99I | CGWCG | 5 cut(s) | 23, 26, 74, 146, 173 |
| HpyAV | CCTTC | 3 cut(s) | 433, 535, 545 |
| HpyCH4III | ACNGT | 1 cut(s) | 227 |
| HpyCH4IV | ACGT | 1 cut(s) | 168 |
| HpyCH4V | TGCA | 3 cut(s) | 266, 296, 470 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 66, 263, 476, 651 |
| HpyF3I | CTNAG | 1 cut(s) | 435 |
| HpySE526I | ACGT | 1 cut(s) | 168 |
| Hsp92I | GRCGYC | 2 cut(s) | 69, 141 |
| Hsp92II | CATG | 2 cut(s) | 296, 460 |
| HspAI | GCGC | 1 cut(s) | 240 |
| KpnI | GGTACC | 1 cut(s) | 226 |
| Kzo9I | GATC | 2 cut(s) | 54, 385 |
| LmnI | GCTCC | 1 cut(s) | 254 |
| Lsp1109I | GCAGC | 1 cut(s) | 632 |
| LweI | GCATC | 1 cut(s) | 354 |
| MaeI | CTAG | 1 cut(s) | 655 |
| MaeII | ACGT | 1 cut(s) | 168 |
| MaeIII | GTNAC | 2 cut(s) | 283, 444 |
| MalI | GATC | 2 cut(s) | 56, 387 |
| MboI | GATC | 2 cut(s) | 54, 385 |
| MboII | GAAGA | 4 cut(s) | 31, 380, 428, 643 |
| MflI | RGATCY | 1 cut(s) | 385 |
| MhlI | GDGCHC | 1 cut(s) | 268 |
| MlsI | TGGCCA | 1 cut(s) | 213 |
| MluCI | AATT | 1 cut(s) | 329 |
| MluNI | TGGCCA | 1 cut(s) | 213 |
| MlyI | GAGTC | 4 cut(s) | 56, 131, 452, 508 |
| MmeI | TCCRAC | 2 cut(s) | 352, 375 |
| MnlI | CCTC | 4 cut(s) | 47, 128, 173, 327 |
| Mox20I | TGGCCA | 1 cut(s) | 213 |
| MroXI | GAANNNNTTC | 2 cut(s) | 110, 571 |
| MscI | TGGCCA | 1 cut(s) | 213 |
| MslI | CAYNNNNRTG | 1 cut(s) | 359 |
| Msp20I | TGGCCA | 1 cut(s) | 213 |
| MspI | CCGG | 2 cut(s) | 6, 66 |
| MspR9I | CCNGG | 2 cut(s) | 7, 94 |
| MvaI | CCWGG | 1 cut(s) | 94 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 66, 263, 476, 651 |
| NciI | CCSGG | 1 cut(s) | 7 |
| NdeII | GATC | 2 cut(s) | 54, 385 |
| NlaIII | CATG | 2 cut(s) | 296, 460 |
| NlaIV | GGNNCC | 2 cut(s) | 224, 338 |
| NmuCI | GTSAC | 2 cut(s) | 283, 444 |
| PaeR7I | CTCGAG | 1 cut(s) | 119 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 27 |
| PdmI | GAANNNNTTC | 2 cut(s) | 110, 571 |
| PfeI | GAWTC | 1 cut(s) | 347 |
| PkrI | GCNGC | 1 cut(s) | 647 |
| PleI | GAGTC | 4 cut(s) | 56, 130, 451, 508 |
| PpsI | GAGTC | 4 cut(s) | 56, 130, 451, 508 |
| Psp6I | CCWGG | 1 cut(s) | 92 |
| PspFI | CCCAGC | 1 cut(s) | 583 |
| PspGI | CCWGG | 1 cut(s) | 92 |
| PspN4I | GGNNCC | 2 cut(s) | 224, 338 |
| PspPI | GGNCC | 1 cut(s) | 190 |
| PspXI | VCTCGAGB | 1 cut(s) | 119 |
| PsuI | RGATCY | 1 cut(s) | 385 |
| RsaI | GTAC | 3 cut(s) | 11, 224, 278 |
| RsaNI | GTAC | 3 cut(s) | 10, 223, 277 |
| RseI | CAYNNNNRTG | 1 cut(s) | 359 |
| SalI | GTCGAC | 1 cut(s) | 170 |
| SatI | GCNGC | 1 cut(s) | 646 |
| Sau3AI | GATC | 2 cut(s) | 54, 385 |
| Sau96I | GGNCC | 1 cut(s) | 190 |
| SchI | GAGTC | 4 cut(s) | 56, 131, 452, 508 |
| ScrFI | CCNGG | 2 cut(s) | 7, 94 |
| SduI | GDGCHC | 1 cut(s) | 268 |
| SfaNI | GCATC | 1 cut(s) | 354 |
| Sfr274I | CTCGAG | 1 cut(s) | 119 |
| SlaI | CTCGAG | 1 cut(s) | 119 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 359 |
| SmlI | CTYRAG | 2 cut(s) | 119, 649 |
| SmoI | CTYRAG | 2 cut(s) | 119, 649 |
| Sse9I | AATT | 1 cut(s) | 329 |
| SspMI | CTAG | 1 cut(s) | 655 |
| StyD4I | CCNGG | 2 cut(s) | 5, 92 |
| TaaI | ACNGT | 1 cut(s) | 227 |
| TaiI | ACGT | 1 cut(s) | 171 |
| TaqI | TCGA | 4 cut(s) | 120, 144, 171, 549 |
| TasI | AATT | 1 cut(s) | 329 |
| TfiI | GAWTC | 1 cut(s) | 347 |
| TscAI | CASTG | 3 cut(s) | 211, 349, 477 |
| TseFI | GTSAC | 2 cut(s) | 283, 444 |
| TseI | GCWGC | 1 cut(s) | 645 |
| Tsp45I | GTSAC | 2 cut(s) | 283, 444 |
| TspDTI | ATGAA | 2 cut(s) | 445, 564 |
| TspRI | CASTG | 3 cut(s) | 211, 349, 477 |
| VneI | GTGCAC | 1 cut(s) | 264 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 41 |
| XhoI | CTCGAG | 1 cut(s) | 119 |
| XmiI | GTMKAC | 1 cut(s) | 171 |
| XmnI | GAANNNNTTC | 2 cut(s) | 110, 571 |
| XspI | CTAG | 1 cut(s) | 655 |