FvH4_6g23332

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000045
Physical Location & Seq
Forward (+)
17352284 .. 17356243
3960 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g23332.t1

Sequence Viewer

Length: 1791 bp
ATGGAGATTCTGGCATGGAATTGTAGGGGAGCCAACAACGATCGGTGTGTCCAAGCGTTGCAAACCCTAATCTGGCAGAAAAAACCCTCCTTCATCTTCCTCTCGGAGACTAAGGTGAATGATGTTGAATACATGAATCAACTGCGTCTCACAATAGGGTTTCACAATTGTGAAACGGTGTATAGTGAGGTATTTGATATTGATGGATTTGGGATTGCTTGGCGTCTAACCAGTTTGTCTGGTGAACCGTCAGCGGGAGACAGACATCTCACTTGGACTCTTCTTCAAGATCTGTTCAAGGCGTCGTCTCTTCCATGGGTGGTGATCGGAGATTTGAATGAGATCTTGAACGATGATGAGAAGGAGGGTGGCCTTCCTCGTAACCCTAACCAGATGCTAAGGTTTCAGGAGGCTCTGGACTCGGTGGAGTTACTGGACCTAGTGTTTCTAAGGGTCCCTTTACCTGGAAGGGCGGTGATGTCAGATGCAGACTTGATCGTGCAGTCTGGTCCGGTTCCGAACTCCAGGAGACCATCTAGGTTCCGTTTTGAATCTTTCTGGTCAACTCACGAAGGTTGTAAGGATGTGGTGTCATCTGGATGGTCTCGGTTTACCCTTGGAAGGCCGATGTATCAAACTATGTCTCGTATTGCTAATACGAGGATAGCTCTCCATCAACGGCAAAAAGTGACTTTTGGATATAGGAAGATGGAGATTGAATTGATCAGAAGTCGGTTACAGCAACTTATGTATCTGCCAATGACTTCGGTTAACCTTGAGGAGCATGCTACTTTATCAAAGAAATTGGAGGGTTTGTTGGAAGAAGAACGCCTTTATTGGATGCAGCGTTCAAAGATTACTTGGCTCACGGTGGGTGACAAGAACACTAAATTCTTCCATAGACAGGCGTCGAACAGGAAAGCCAAGAATCGACTCATTGGTCTATTTGATGAATTGGGGATTTGGAAACCGGCTATTAATGGGATGGAGCAGATAATTTTGAAGTACTTTTCGAATGTGTTTCGCTTTGAACCAGTTGATATGGGGCGTATGCAGTCTATTGTGAACTTGGTACAACCGAGGGTAGCTGAAGCAATGAATGAGGATTTGTGTGCTTCTTATTCGGATAGTGAGATTCGTGCTGCTCTATTTCAAATCAAATGGCAGATTGGTGATGGGGTTTCGGTTGATGCTTGGAACGATCCTTGGCTACAGAGGCTGAGAGATTTCCGTCCTCTCAGTAGGCTCAGTCGCAGCGCTTCAAAGAAAGGTATATGGTTTTCAGCTATTTCGAGCAAGATCAAGTTGAGAAGGGTGCTTCAGGGATGGATTCTGCCTGTGGTTGAGTGGCTTTCTATGTGCATCGAGGTTCTAATTAAGGAAGAGTTTGTTCTATTGTTGGTCATTCTTTGGTCAGTATGGAGAGAGCGTAATCTCAGGCTTTGGGATGGTAAGTACAGGTTATGTTCCCAGCTATACTATCAGGGGGGAGTAGGGGTGATTTTCAAAGACTCTCATTCAAAAGTTATTGGAGGTCAGTATTGCAGAGTGGAGCATGTGTCTTCGCCTGAGATGGTTGAGGCTATGGCGGGTAGGTTTGCATGTGAAGCAGCTCAGGTGTTGCTGGTGTTCCCAGTTGTTTTTGAATCTGATTGTTTGAAGCTTGTAAAAGCTACAAAGTGTGAAGATGTGGATGATTCGGCTTTTGGTGTCATTGTTGAGGACATTAAGCAGATCTTTGTCATCTTAATTCATCATTTTTCTCTGATGTTTATAGGGAGTCCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

597

Amino Acids

68.73

Weight (kDa)

8.37

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 5 - 131 9.1e-09 Endonuclease/Exonuclease/phosphatase family
RVT_3 PF13456 494 - 579 3e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000045)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20031 FvH4_1g20032 FvH4_1g28811 FvH4_2g01463 FvH4_2g09182 FvH4_2g28261 FvH4_3g29411 FvH4_3g37471 FvH4_4g14101 FvH4_4g16371 FvH4_4g18821 FvH4_5g18832 FvH4_5g30171 FvH4_6g12302 FvH4_6g16683 FvH4_6g16753 FvH4_6g17021 FvH4_6g18902 FvH4_6g19251 FvH4_6g23332 FvH4_6g28111 FvH4_6g33201 FvH4_6g40361 FvH4_7g09171
prunus_persica Prupe.1G074300_v2.0.a1 Prupe.3G105600_v2.0.a1
pyrus_communis pycom05g10560 pycom07g05620 pycom12424g00110
rosa_chinensis RchiOBHm_Chr1g0334141 RchiOBHm_Chr5g0016671 RchiOBHm_Chr7g0208511 RchiOBHm_Chr7g0231041
rosa_laevigata RLG00000015243
rosa_multiflora Rmu_co7986832.1_g000001 Rmu_co8043772.1_g000001 Rmu_co8043774.1_g000001 Rmu_co8055874.1_g000001 Rmu_co8106278.1_g000001 Rmu_co8122148.1_g000001 Rmu_co8133260.1_g000001 Rmu_co8134318.1_g000001 Rmu_co8183860.1_g000001 Rmu_co8184294.1_g000001 Rmu_co8189556.1_g000001 Rmu_co8236577.1_g000001 Rmu_co8239421.1_g000001 Rmu_co8250871.1_g000001 Rmu_co8300345.1_g000001 Rmu_co8310915.1_g000001 Rmu_co8333321.1_g000001 Rmu_co8334427.1_g000001 Rmu_co8360619.1_g000001 Rmu_co8360619.1_g000002 Rmu_co8366931.1_g000001 Rmu_co8375289.1_g000001 Rmu_co8406881.1_g000001 Rmu_co8444187.1_g000001 Rmu_co8464563.1_g000001 Rmu_co8505097.1_g000001 Rmu_sc0000031.1_g000042 Rmu_sc0000031.1_g000043 Rmu_sc0000053.1_g000008 Rmu_sc0000104.1_g000026 Rmu_sc0000115.1_g000003 Rmu_sc0000148.1_g000077 Rmu_sc0000257.1_g000001 Rmu_sc0000311.1_g000016 Rmu_sc0000347.1_g000035 Rmu_sc0000365.1_g000048 Rmu_sc0000389.1_g000027 Rmu_sc0000399.1_g000009 Rmu_sc0000399.1_g000015 Rmu_sc0000408.1_g000016 Rmu_sc0000452.1_g000007 Rmu_sc0000458.1_g000015 Rmu_sc0000470.1_g000010 Rmu_sc0000498.1_g000067 Rmu_sc0000499.1_g000016 Rmu_sc0000536.1_g000029 Rmu_sc0000539.1_g000015 Rmu_sc0000544.1_g000005 Rmu_sc0000571.1_g000013 Rmu_sc0000573.1_g000013 Rmu_sc0000573.1_g000014 Rmu_sc0000600.1_g000043 Rmu_sc0000631.1_g000014 Rmu_sc0000657.1_g000014 Rmu_sc0000711.1_g000005 Rmu_sc0000711.1_g000059 Rmu_sc0000717.1_g000024 Rmu_sc0000717.1_g000025 Rmu_sc0000737.1_g000001 Rmu_sc0000744.1_g000081 Rmu_sc0000757.1_g000011 Rmu_sc0000784.1_g000026 Rmu_sc0000800.1_g000010 Rmu_sc0000816.1_g000017 Rmu_sc0000816.1_g000022 Rmu_sc0000870.1_g000047 Rmu_sc0000898.1_g000107 Rmu_sc0000929.1_g000011 Rmu_sc0001009.1_g000034 Rmu_sc0001059.1_g000021 Rmu_sc0001062.1_g000003 Rmu_sc0001124.1_g000027 Rmu_sc0001169.1_g000015 Rmu_sc0001169.1_g000016 Rmu_sc0001176.1_g000011 Rmu_sc0001176.1_g000012 Rmu_sc0001200.1_g000058 Rmu_sc0001200.1_g000059 Rmu_sc0001211.1_g000132 Rmu_sc0001250.1_g000019 Rmu_sc0001288.1_g000007 Rmu_sc0001416.1_g000012 Rmu_sc0001455.1_g000033 Rmu_sc0001461.1_g000077 Rmu_sc0001514.1_g000008 Rmu_sc0001531.1_g000015 Rmu_sc0001531.1_g000016 Rmu_sc0001548.1_g000037 Rmu_sc0001576.1_g000031 Rmu_sc0001615.1_g000011 Rmu_sc0001648.1_g000052 Rmu_sc0001648.1_g000053 Rmu_sc0001694.1_g000025 Rmu_sc0001696.1_g000022 Rmu_sc0001716.1_g000025 Rmu_sc0001799.1_g000003 Rmu_sc0001801.1_g000010 Rmu_sc0001801.1_g000011 Rmu_sc0001814.1_g000007 Rmu_sc0001863.1_g000030 Rmu_sc0001891.1_g000056 Rmu_sc0001891.1_g000057 Rmu_sc0001891.1_g000058 Rmu_sc0001940.1_g000032 Rmu_sc0001966.1_g000020 Rmu_sc0001982.1_g000009 Rmu_sc0001998.1_g000020 Rmu_sc0001998.1_g000021 Rmu_sc0002000.1_g000008 Rmu_sc0002023.1_g000013 Rmu_sc0002038.1_g000015 Rmu_sc0002038.1_g000016 Rmu_sc0002043.1_g000008 Rmu_sc0002063.1_g000010 Rmu_sc0002094.1_g000035 Rmu_sc0002099.1_g000007 Rmu_sc0002300.1_g000012 Rmu_sc0002316.1_g000063 Rmu_sc0002485.1_g000006 Rmu_sc0002520.1_g000010 Rmu_sc0002539.1_g000041 Rmu_sc0002665.1_g000012 Rmu_sc0002858.1_g000005 Rmu_sc0002882.1_g000038 Rmu_sc0002882.1_g000039 Rmu_sc0002890.1_g000016 Rmu_sc0002931.1_g000024 Rmu_sc0002942.1_g000022 Rmu_sc0003002.1_g000051 Rmu_sc0003012.1_g000030 Rmu_sc0003147.1_g000014 Rmu_sc0003156.1_g000006 Rmu_sc0003162.1_g000006 Rmu_sc0003261.1_g000027 Rmu_sc0003399.1_g000008 Rmu_sc0003500.1_g000004 Rmu_sc0003514.1_g000006 Rmu_sc0003764.1_g000013 Rmu_sc0003764.1_g000014 Rmu_sc0003764.1_g000015 Rmu_sc0003776.1_g000039 Rmu_sc0003829.1_g000001 Rmu_sc0003834.1_g000016 Rmu_sc0003834.1_g000017 Rmu_sc0003834.1_g000019 Rmu_sc0003834.1_g000020 Rmu_sc0003929.1_g000011 Rmu_sc0004003.1_g000002 Rmu_sc0004005.1_g000002 Rmu_sc0004240.1_g000001 Rmu_sc0004240.1_g000002 Rmu_sc0004340.1_g000023 Rmu_sc0004373.1_g000008 Rmu_sc0004373.1_g000009 Rmu_sc0004413.1_g000030 Rmu_sc0004467.1_g000011 Rmu_sc0004473.1_g000010 Rmu_sc0004577.1_g000023 Rmu_sc0004596.1_g000040 Rmu_sc0004711.1_g000024 Rmu_sc0004714.1_g000005 Rmu_sc0004720.1_g000012 Rmu_sc0004730.1_g000002 Rmu_sc0004733.1_g000004 Rmu_sc0004763.1_g000001 Rmu_sc0004768.1_g000010 Rmu_sc0004889.1_g000007 Rmu_sc0004889.1_g000008 Rmu_sc0004904.1_g000005 Rmu_sc0004961.1_g000003 Rmu_sc0004961.1_g000009 Rmu_sc0004990.1_g000007 Rmu_sc0005104.1_g000004 Rmu_sc0005122.1_g000016 Rmu_sc0005122.1_g000017 Rmu_sc0005229.1_g000013 Rmu_sc0005308.1_g000009 Rmu_sc0005363.1_g000010 Rmu_sc0005363.1_g000011 Rmu_sc0005382.1_g000001 Rmu_sc0005389.1_g000023 Rmu_sc0005514.1_g000008 Rmu_sc0005593.1_g000014 Rmu_sc0005705.1_g000010 Rmu_sc0005722.1_g000010 Rmu_sc0005745.1_g000027 Rmu_sc0005745.1_g000028 Rmu_sc0005802.1_g000023 Rmu_sc0005899.1_g000002 Rmu_sc0005952.1_g000001 Rmu_sc0005978.1_g000007 Rmu_sc0006158.1_g000001 Rmu_sc0006233.1_g000035 Rmu_sc0006343.1_g000009 Rmu_sc0006367.1_g000005 Rmu_sc0006444.1_g000007 Rmu_sc0006445.1_g000017 Rmu_sc0006493.1_g000015 Rmu_sc0006552.1_g000002 Rmu_sc0006695.1_g000054 Rmu_sc0006720.1_g000006 Rmu_sc0006722.1_g000015 Rmu_sc0007029.1_g000016 Rmu_sc0007080.1_g000001 Rmu_sc0007328.1_g000007 Rmu_sc0007341.1_g000003 Rmu_sc0007366.1_g000006 Rmu_sc0007421.1_g000003 Rmu_sc0007652.1_g000013 Rmu_sc0007716.1_g000002 Rmu_sc0007790.1_g000016 Rmu_sc0007802.1_g000002 Rmu_sc0008049.1_g000037 Rmu_sc0008087.1_g000009 Rmu_sc0008509.1_g000006 Rmu_sc0008676.1_g000002 Rmu_sc0008800.1_g000012 Rmu_sc0009053.1_g000003 Rmu_sc0009053.1_g000004 Rmu_sc0009241.1_g000002 Rmu_sc0009538.1_g000016 Rmu_sc0009568.1_g000001 Rmu_sc0009579.1_g000014 Rmu_sc0009742.1_g000001 Rmu_sc0009895.1_g000025 Rmu_sc0010202.1_g000006 Rmu_sc0010334.1_g000001 Rmu_sc0010483.1_g000022 Rmu_sc0010901.1_g000001 Rmu_sc0010937.1_g000008 Rmu_sc0011079.1_g000001 Rmu_sc0011173.1_g000012 Rmu_sc0011609.1_g000001 Rmu_sc0011698.1_g000013 Rmu_sc0011778.1_g000018 Rmu_sc0012115.1_g000006 Rmu_sc0012403.1_g000018 Rmu_sc0012426.1_g000003 Rmu_sc0012426.1_g000004 Rmu_sc0012705.1_g000001 Rmu_sc0012897.1_g000003 Rmu_sc0013014.1_g000009 Rmu_sc0013188.1_g000003 Rmu_sc0013612.1_g000007 Rmu_sc0013612.1_g000008 Rmu_sc0014076.1_g000002 Rmu_sc0014162.1_g000018 Rmu_sc0014206.1_g000008 Rmu_sc0014426.1_g000003 Rmu_sc0015447.1_g000006 Rmu_sc0016197.1_g000003 Rmu_sc0016546.1_g000001 Rmu_sc0016546.1_g000002 Rmu_sc0017848.1_g000001 Rmu_sc0017848.1_g000002 Rmu_sc0018300.1_g000001 Rmu_sc0018325.1_g000008 Rmu_sc0019396.1_g000002 Rmu_sc0019815.1_g000003 Rmu_sc0022463.1_g000004 Rmu_sc0023070.1_g000009 Rmu_sc0023965.1_g000004 Rmu_sc0024072.1_g000004 Rmu_sc0024195.1_g000017 Rmu_sc0024446.1_g000004 Rmu_sc0026134.1_g000001 Rmu_sc0026279.1_g000001 Rmu_sc0026736.1_g000001 Rmu_sc0027357.1_g000001 Rmu_sc0027357.1_g000002 Rmu_sc0027596.1_g000001 Rmu_sc0027596.1_g000002 Rmu_sc0027778.1_g000005 Rmu_sc0027873.1_g000001 Rmu_sc0028295.1_g000001 Rmu_sc0028706.1_g000002 Rmu_sc0034015.1_g000001 Rmu_sc0034598.1_g000002 Rmu_sc0035534.1_g000001 Rmu_sc0036127.1_g000001 Rmu_sc0036172.1_g000001 Rmu_sc0036279.1_g000002 Rmu_sc0036593.1_g000001 Rmu_sc0036891.1_g000001 Rmu_sc0041752.1_g000001 Rmu_sc0041761.1_g000001 Rmu_sc0042299.1_g000001 Rmu_sc0042653.1_g000003 Rmu_ssc0000011.1_g000004 Rmu_ssc0000035.1_g000004 Rmu_ssc0000064.1_g000029 Rmu_ssc0000201.1_g000005 Rmu_ssc0000201.1_g000010 Rmu_ssc0000201.1_g000011 Rmu_ssc0000342.1_g000008 Rmu_ssc0000342.1_g000019 Rmu_ssc0000381.1_g000002 Rmu_ssc0000432.1_g000083
rosa_roxburghii Rroxscaffold_1G00000890 Rroxscaffold_1G00045620 Rroxscaffold_1G00061860 Rroxscaffold_4G00303310 Rroxscaffold_4G00331420 Rroxscaffold_6G00406060
rosa_rugosa Rorug01G0072600 Rorug01G0113600 Rorug01G0113700 Rorug01G0167100 Rorug01G0199300 Rorug01G0274300 Rorug01G0312600 Rorug01G0383700 Rorug02G0026100.1 Rorug02G0173700 Rorug02G0211900 Rorug02G0212100 Rorug02G0341000 Rorug02G0634200 Rorug03G0098500 Rorug03G0106400 Rorug03G0144900 Rorug03G0145000 Rorug03G0155700 Rorug03G0155800 Rorug03G0170300 Rorug03G0184200 Rorug03G0213700 Rorug03G0229300 Rorug03G0232700.1 Rorug03G0232800 Rorug03G0324800 Rorug03G0334900 Rorug04G0049700 Rorug04G0067800 Rorug04G0197400 Rorug04G0235600 Rorug05G0038600 Rorug05G0081400 Rorug05G0089200 Rorug05G0157400 Rorug05G0157900 Rorug05G0157900 Rorug05G0186600 Rorug05G0214300 Rorug05G0267700 Rorug05G0309100 Rorug05G0370800 Rorug05G0419000 Rorug05G0515800 Rorug06G0040600 Rorug06G0069100 Rorug06G0142200 Rorug06G0142800 Rorug06G0144400 Rorug06G0276500 Rorug06G0444200 Rorug06G0495800 Rorug07G0017900 Rorug07G0030300 Rorug07G0031900 Rorug07G0032000 Rorug07G0051000 Rorug07G0053700 Rorug07G0116200 Rorug07G0164400 Rorug07G0236200 Rorug07G0308600 Rorug07G0313900
rosa_samantha Rh1CG051600 Rh1CG098200 Rh1CG101900 Rh1DG109900 Rh2BG354600 Rh2CG366900 Rh2DG048400 Rh2DG464500 Rh2DG484000 Rh3AG277100 Rh3AG286800 Rh3BG229500 Rh3DG264200 Rh4BG155600 Rh5AG127200 Rh5AG520800 Rh5BG116100 Rh6DG091200 Rh6DG091300 Rh7AG106700 Rh7AG343500 Rh7BG179600 Rh7BG337400 Rh7CG299700
rosa_wichuraiana Rw1G026260 Rw5G047800 Rw6G003270 Rw6G025380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 939
AciI CCGC 3 cut(s) 254, 473, 1589
AclWI GGATC 1 cut(s) 1196
AcsI RAATTY 1 cut(s) 890
AcuI CTGAAG 2 cut(s) 1110, 1304
AcyI GRCGYC 3 cut(s) 223, 302, 908
AfaI GTAC 3 cut(s) 1007, 1074, 1457
AfeI AGCGCT 1 cut(s) 1258
AfiI CCNNNNNNNGG 5 cut(s) 72, 254, 464, 621, 904
AjnI CCWGG 2 cut(s) 463, 524
AleI CACNNNNGTG 1 cut(s) 168
AloI GAACNNNNNNTCC 2 cut(s) 832, 864
AluBI AGCT 7 cut(s) 668, 1088, 1286, 1474, 1613, 1663, 1673
AluI AGCT 7 cut(s) 668, 1088, 1286, 1474, 1613, 1663, 1673
Alw26I GTCTC 7 cut(s) 101, 152, 252, 312, 523, 609, 648
AlwI GGATC 1 cut(s) 1196
AlwNI CAGNNNCTG 1 cut(s) 1219
Aor51HI AGCGCT 1 cut(s) 1258
AoxI GGCC 2 cut(s) 370, 623
ApeKI GCWGC 4 cut(s) 844, 1142, 1254, 1610
ApoI RAATTY 1 cut(s) 890
AseI ATTAAT 1 cut(s) 978
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 3 cut(s) 436, 454, 509
AsuHPI GGTGA 7 cut(s) 127, 254, 334, 487, 887, 1184, 1510
AsuII TTCGAA 1 cut(s) 1013
AvaII GGWCC 3 cut(s) 436, 454, 509
BaeI ACNNNNGTAYC 2 cut(s) 734, 767
BbsI GAAGAC 1 cut(s) 1554
BbvI GCAGC 4 cut(s) 856, 1129, 1266, 1622
BceAI ACGGC 1 cut(s) 695
BciT130I CCWGG 2 cut(s) 465, 526
BclI TGATCA 1 cut(s) 723
BcoDI GTCTC 7 cut(s) 101, 152, 252, 312, 523, 609, 648
BfaI CTAG 2 cut(s) 440, 537
BfmI CTRYAG 1 cut(s) 1211
BfoI RGCGCY 1 cut(s) 1260
BglII AGATCT 3 cut(s) 289, 342, 1734
BisI GCNGC 4 cut(s) 845, 1143, 1255, 1611
BlsI GCNGC 4 cut(s) 846, 1144, 1256, 1612
BmcAI AGTACT 1 cut(s) 1007
Bme1390I CCNGG 2 cut(s) 465, 526
Bme18I GGWCC 3 cut(s) 436, 454, 509
BmgT120I GGNCC 3 cut(s) 436, 454, 509
BmiI GGNNCC 5 cut(s) 31, 455, 456, 516, 542
BmrFI CCNGG 2 cut(s) 465, 526
BmrI ACTGGG 1 cut(s) 1628
BmsI GCATC 5 cut(s) 384, 475, 831, 1180, 1371
BmuI ACTGGG 1 cut(s) 1628
BoxI GACNNNNGTC 1 cut(s) 907
BpiI GAAGAC 1 cut(s) 1554
BplI GAGNNNNNCTC 2 cut(s) 652, 684
BpmI CTGGAG 1 cut(s) 508
Bpu10I CCTNAGC 2 cut(s) 398, 1614
Bpu14I TTCGAA 1 cut(s) 1013
BpuEI CTTGAG 1 cut(s) 797
BsaHI GRCGYC 3 cut(s) 223, 302, 908
BsaI GGTCTC 2 cut(s) 523, 609
BsaJI CCNNGG 4 cut(s) 314, 616, 1079, 1205
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 8 cut(s) 800, 830, 1414, 1444, 1482, 1512, 1544, 1574
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 254, 464, 621, 904
Bse118I RCCGGY 1 cut(s) 970
Bse1I ACTGG 4 cut(s) 231, 438, 1034, 1634
Bse3DI GCAATG 1 cut(s) 1101
BseBI CCWGG 2 cut(s) 465, 526
BseDI CCNNGG 4 cut(s) 314, 616, 1079, 1205
BseGI GGATG 7 cut(s) 589, 605, 846, 990, 1331, 1453, 1699
BseLI CCNNNNNNNGG 5 cut(s) 72, 254, 464, 621, 904
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 6 cut(s) 1211, 1252, 1261, 1450, 1560, 1628
BseNI ACTGG 4 cut(s) 231, 438, 1034, 1634
BseRI GAGGAG 1 cut(s) 794
BseXI GCAGC 4 cut(s) 856, 1129, 1266, 1622
BseYI CCCAGC 1 cut(s) 1470
BsgI GTGCAG 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 2 cut(s) 372, 625
BsiEI CGRYCG 1 cut(s) 43
BsiSI CCGG 2 cut(s) 512, 971
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 5 cut(s) 72, 254, 464, 621, 904
BsmAI GTCTC 7 cut(s) 101, 152, 252, 312, 523, 609, 648
BsmBI CGTCTC 2 cut(s) 152, 312
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 2 cut(s) 372, 625
Bso31I GGTCTC 2 cut(s) 523, 609
Bsp119I TTCGAA 1 cut(s) 1013
Bsp143I GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 3 cut(s) 254, 473, 1589
BspANI GGCC 2 cut(s) 372, 625
BspCNI CTCAG 6 cut(s) 1212, 1251, 1260, 1449, 1561, 1627
BspLI GGNNCC 5 cut(s) 31, 455, 456, 516, 542
BspPI GGATC 1 cut(s) 1196
BspT104I TTCGAA 1 cut(s) 1013
BspTNI GGTCTC 2 cut(s) 523, 609
BsrDI GCAATG 1 cut(s) 1101
BsrFI RCCGGY 1 cut(s) 970
BsrI ACTGG 4 cut(s) 231, 438, 1034, 1634
BssAI RCCGGY 1 cut(s) 970
BssECI CCNNGG 4 cut(s) 314, 616, 1079, 1205
BssMI GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
BssNI GRCGYC 3 cut(s) 223, 302, 908
BssT1I CCWWGG 3 cut(s) 314, 616, 1205
Bst2UI CCWGG 2 cut(s) 465, 526
Bst4CI ACNGT 3 cut(s) 178, 249, 871
Bst6I CTCTTC 3 cut(s) 285, 315, 1377
BstACI GRCGYC 3 cut(s) 223, 302, 908
BstBI TTCGAA 1 cut(s) 1013
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 9 cut(s) 111, 398, 449, 1220, 1238, 1247, 1436, 1569, 1614
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 7 cut(s) 589, 605, 846, 990, 1331, 1453, 1699
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 9 cut(s) 43, 292, 327, 345, 498, 726, 1204, 1302, 1737
BstMAI GTCTC 7 cut(s) 101, 152, 252, 312, 523, 609, 648
BstMBI GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 1216, 1607
BstNI CCWGG 2 cut(s) 465, 526
BstNSI RCATGY 3 cut(s) 788, 1559, 1605
BstPAI GACNNNNGTC 1 cut(s) 907
BstSCI CCNGG 2 cut(s) 463, 524
BstSFI CTRYAG 1 cut(s) 1211
BstV1I GCAGC 4 cut(s) 856, 1129, 1266, 1622
BstV2I GAAGAC 1 cut(s) 1554
BstX2I RGATCY 3 cut(s) 289, 342, 1734
BstYI RGATCY 3 cut(s) 289, 342, 1734
BsuRI GGCC 2 cut(s) 372, 625
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 7 cut(s) 589, 605, 846, 990, 1331, 1453, 1699
Cac8I GCNNGC 1 cut(s) 786
CaiI CAGNNNCTG 1 cut(s) 1219
CfoI GCGC 1 cut(s) 1259
Cfr10I RCCGGY 1 cut(s) 970
Cfr13I GGNCC 3 cut(s) 436, 454, 509
CseI GACGC 4 cut(s) 134, 212, 291, 897
Csp6I GTAC 3 cut(s) 1006, 1073, 1456
CviAII CATG 6 cut(s) 15, 133, 315, 785, 1556, 1602
CviQI GTAC 3 cut(s) 1006, 1073, 1456
DdeI CTNAG 9 cut(s) 111, 398, 449, 1220, 1238, 1247, 1436, 1569, 1614
DpnI GATC 9 cut(s) 42, 291, 326, 344, 497, 725, 1203, 1301, 1736
DpnII GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
DrdI GACNNNNNNGTC 1 cut(s) 939
DseDI GACNNNNNNGTC 1 cut(s) 939
Eam1104I CTCTTC 3 cut(s) 285, 315, 1377
EarI CTCTTC 3 cut(s) 285, 315, 1377
Eco130I CCWWGG 3 cut(s) 314, 616, 1205
Eco31I GGTCTC 2 cut(s) 523, 609
Eco47I GGWCC 3 cut(s) 436, 454, 509
Eco47III AGCGCT 1 cut(s) 1258
Eco57I CTGAAG 2 cut(s) 1110, 1304
EcoO109I RGGNCCY 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 463, 524
EcoT14I CCWWGG 3 cut(s) 314, 616, 1205
ErhI CCWWGG 3 cut(s) 314, 616, 1205
Esp3I CGTCTC 2 cut(s) 152, 312
FaeI CATG 6 cut(s) 18, 136, 318, 788, 1559, 1605
FalI AAGNNNNNCTT 6 cut(s) 442, 474, 816, 848, 1721, 1753
FaqI GGGAC 1 cut(s) 440
FatI CATG 6 cut(s) 14, 132, 314, 784, 1555, 1601
FauI CCCGC 2 cut(s) 247, 1582
FbaI TGATCA 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 845, 1143, 1255, 1611
FokI GGATG 7 cut(s) 596, 612, 853, 997, 1338, 1460, 1706
Fsp4HI GCNGC 4 cut(s) 845, 1143, 1255, 1611
FspBI CTAG 2 cut(s) 440, 537
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 4 cut(s) 845, 1143, 1255, 1611
GsaI CCCAGC 1 cut(s) 1474
GsuI CTGGAG 1 cut(s) 508
HaeII RGCGCY 1 cut(s) 1260
HaeIII GGCC 2 cut(s) 372, 625
HapII CCGG 2 cut(s) 512, 971
HgaI GACGC 4 cut(s) 134, 212, 291, 897
HhaI GCGC 1 cut(s) 1259
Hin1I GRCGYC 3 cut(s) 223, 302, 908
Hin1II CATG 6 cut(s) 18, 136, 318, 788, 1559, 1605
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HincII GTYRAC 2 cut(s) 564, 772
HindII GTYRAC 2 cut(s) 564, 772
HindIII AAGCTT 1 cut(s) 1661
HpaI GTTAAC 1 cut(s) 772
HpaII CCGG 2 cut(s) 512, 971
HphI GGTGA 7 cut(s) 127, 254, 334, 487, 887, 1184, 1510
Hpy166II GTNNAC 5 cut(s) 245, 564, 612, 772, 1066
Hpy188I TCNGA 8 cut(s) 106, 329, 484, 519, 728, 1126, 1651, 1767
Hpy188III TCNNGA 6 cut(s) 287, 346, 407, 416, 569, 597
Hpy8I GTNNAC 5 cut(s) 245, 564, 612, 772, 1066
Hpy99I CGWCG 2 cut(s) 307, 913
HpyAV CCTTC 7 cut(s) 100, 355, 383, 462, 566, 615, 1305
HpyCH4III ACNGT 3 cut(s) 178, 249, 871
HpyCH4V TGCA 8 cut(s) 61, 488, 502, 844, 1054, 1362, 1545, 1601
HpyF10VI GCNNNNNNNGC 2 cut(s) 1216, 1607
HpyF3I CTNAG 9 cut(s) 111, 398, 449, 1220, 1238, 1247, 1436, 1569, 1614
Hsp92I GRCGYC 3 cut(s) 223, 302, 908
Hsp92II CATG 6 cut(s) 18, 136, 318, 788, 1559, 1605
HspAI GCGC 1 cut(s) 1257
KflI GGGWCCC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 723
KspAI GTTAAC 1 cut(s) 772
Kzo9I GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
LmnI GCTCC 4 cut(s) 29, 781, 988, 1552
Lsp1109I GCAGC 4 cut(s) 856, 1129, 1266, 1622
LweI GCATC 5 cut(s) 384, 475, 831, 1180, 1371
MaeI CTAG 2 cut(s) 440, 537
MaeIII GTNAC 5 cut(s) 380, 429, 688, 735, 875
MalI GATC 9 cut(s) 42, 291, 326, 344, 497, 725, 1203, 1301, 1736
MboI GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
MfeI CAATTG 1 cut(s) 166
MflI RGATCY 3 cut(s) 289, 342, 1734
MlyI GAGTC 5 cut(s) 271, 413, 927, 1505, 1789
MmeI TCCRAC 1 cut(s) 798
MseI TTAA 6 cut(s) 771, 978, 1377, 1728, 1748, 1789
MslI CAYNNNNRTG 2 cut(s) 168, 598
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 512, 971
MspR9I CCNGG 2 cut(s) 465, 526
MunI CAATTG 1 cut(s) 166
MvaI CCWGG 2 cut(s) 465, 526
MwoI GCNNNNNNNGC 2 cut(s) 1216, 1607
NcoI CCATGG 1 cut(s) 314
NdeII GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
NlaIII CATG 6 cut(s) 18, 136, 318, 788, 1559, 1605
NlaIV GGNNCC 5 cut(s) 31, 455, 456, 516, 542
NmuCI GTSAC 2 cut(s) 688, 875
NspI RCATGY 3 cut(s) 788, 1559, 1605
NspV TTCGAA 1 cut(s) 1013
OliI CACNNNNGTG 1 cut(s) 168
PaeI GCATGC 1 cut(s) 788
PfeI GAWTC 8 cut(s) 7, 136, 551, 928, 1135, 1330, 1646, 1697
PfoI TCCNGGA 1 cut(s) 524
PkrI GCNGC 4 cut(s) 846, 1144, 1256, 1612
Ple19I CGATCG 1 cut(s) 43
PleI GAGTC 5 cut(s) 271, 413, 927, 1505, 1788
PpsI GAGTC 5 cut(s) 271, 413, 927, 1505, 1788
PpuMI RGGWCCY 1 cut(s) 454
PshAI GACNNNNGTC 1 cut(s) 907
PshBI ATTAAT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 2 cut(s) 463, 524
PspFI CCCAGC 1 cut(s) 1470
PspGI CCWGG 2 cut(s) 463, 524
PspN4I GGNNCC 5 cut(s) 31, 455, 456, 516, 542
PspPI GGNCC 3 cut(s) 436, 454, 509
PspPPI RGGWCCY 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 1219
PsuI RGATCY 3 cut(s) 289, 342, 1734
PvuI CGATCG 1 cut(s) 43
RsaI GTAC 3 cut(s) 1007, 1074, 1457
RsaNI GTAC 3 cut(s) 1006, 1073, 1456
RseI CAYNNNNRTG 2 cut(s) 168, 598
SaqAI TTAA 6 cut(s) 771, 978, 1377, 1728, 1748, 1789
SatI GCNGC 4 cut(s) 845, 1143, 1255, 1611
Sau3AI GATC 9 cut(s) 40, 289, 324, 342, 495, 723, 1201, 1299, 1734
Sau96I GGNCC 3 cut(s) 436, 454, 509
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 5 cut(s) 271, 413, 927, 1505, 1789
ScrFI CCNGG 2 cut(s) 465, 526
SfaNI GCATC 5 cut(s) 384, 475, 831, 1180, 1371
SfcI CTRYAG 1 cut(s) 1211
SfuI TTCGAA 1 cut(s) 1013
SinI GGWCC 3 cut(s) 436, 454, 509
SmiMI CAYNNNNRTG 2 cut(s) 168, 598
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
SphI GCATGC 1 cut(s) 788
SsiI CCGC 3 cut(s) 254, 473, 1589
SspMI CTAG 2 cut(s) 440, 537
StyD4I CCNGG 2 cut(s) 463, 524
StyI CCWWGG 3 cut(s) 314, 616, 1205
TaaI ACNGT 3 cut(s) 178, 249, 871
TaqI TCGA 5 cut(s) 911, 931, 1013, 1292, 1365
TatI WGTACW 2 cut(s) 1005, 1455
TfiI GAWTC 8 cut(s) 7, 136, 551, 928, 1135, 1330, 1646, 1697
Tru1I TTAA 6 cut(s) 771, 978, 1377, 1728, 1748, 1789
Tru9I TTAA 6 cut(s) 771, 978, 1377, 1728, 1748, 1789
TseFI GTSAC 2 cut(s) 688, 875
TseI GCWGC 4 cut(s) 844, 1142, 1254, 1610
Tsp45I GTSAC 2 cut(s) 688, 875
TspDTI ATGAA 5 cut(s) 82, 149, 966, 1112, 1742
TspGWI ACGGA 2 cut(s) 533, 1220
VpaK11BI GGWCC 3 cut(s) 436, 454, 509
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 1 cut(s) 890
XceI RCATGY 3 cut(s) 788, 1559, 1605
XspI CTAG 2 cut(s) 440, 537
ZrmI AGTACT 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.