ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 4 cut(s) | 247, 296, 309, 421 |
| AciI | CCGC | 5 cut(s) | 97, 128, 661, 694, 736 |
| AclWI | GGATC | 1 cut(s) | 159 |
| AdeI | CACNNNGTG | 1 cut(s) | 53 |
| AfaI | GTAC | 3 cut(s) | 198, 546, 615 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 127, 190, 523, 700 |
| AflII | CTTAAG | 1 cut(s) | 680 |
| AgsI | TTSAA | 6 cut(s) | 61, 160, 358, 365, 511, 827 |
| AjiI | CACGTC | 2 cut(s) | 7, 53 |
| AjnI | CCWGG | 4 cut(s) | 138, 146, 312, 579 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 28 |
| AluBI | AGCT | 3 cut(s) | 345, 557, 671 |
| AluI | AGCT | 3 cut(s) | 345, 557, 671 |
| AlwI | GGATC | 1 cut(s) | 159 |
| AoxI | GGCC | 1 cut(s) | 663 |
| ApeKI | GCWGC | 2 cut(s) | 436, 496 |
| AseI | ATTAAT | 1 cut(s) | 608 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 743 |
| AspS9I | GGNCC | 3 cut(s) | 514, 663, 691 |
| AsuHPI | GGTGA | 1 cut(s) | 215 |
| AsuII | TTCGAA | 1 cut(s) | 455 |
| AvaII | GGWCC | 2 cut(s) | 514, 691 |
| BbsI | GAAGAC | 1 cut(s) | 499 |
| BbvI | GCAGC | 2 cut(s) | 448, 508 |
| BccI | CCATC | 2 cut(s) | 236, 357 |
| BciT130I | CCWGG | 4 cut(s) | 140, 148, 314, 581 |
| BfaI | CTAG | 2 cut(s) | 474, 551 |
| BfrI | CTTAAG | 1 cut(s) | 680 |
| BglII | AGATCT | 1 cut(s) | 216 |
| BisI | GCNGC | 3 cut(s) | 129, 437, 497 |
| BlsI | GCNGC | 3 cut(s) | 130, 438, 498 |
| Bme1390I | CCNGG | 4 cut(s) | 140, 148, 314, 581 |
| Bme18I | GGWCC | 2 cut(s) | 514, 691 |
| BmgBI | CACGTC | 2 cut(s) | 7, 53 |
| BmgT120I | GGNCC | 3 cut(s) | 514, 663, 691 |
| BmiI | GGNNCC | 1 cut(s) | 692 |
| BmrFI | CCNGG | 4 cut(s) | 140, 148, 314, 581 |
| BmrI | ACTGGG | 1 cut(s) | 209 |
| BmsI | GCATC | 5 cut(s) | 152, 236, 274, 388, 478 |
| BmuI | ACTGGG | 1 cut(s) | 209 |
| BoxI | GACNNNNGTC | 2 cut(s) | 253, 390 |
| BpiI | GAAGAC | 1 cut(s) | 499 |
| Bpu14I | TTCGAA | 1 cut(s) | 455 |
| Bsa29I | ATCGAT | 1 cut(s) | 352 |
| BsaJI | CCNNGG | 5 cut(s) | 147, 188, 251, 313, 579 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 127, 190, 523, 700 |
| Bse1I | ACTGG | 2 cut(s) | 204, 309 |
| Bse3DI | GCAATG | 1 cut(s) | 477 |
| BseBI | CCWGG | 4 cut(s) | 140, 148, 314, 581 |
| BseCI | ATCGAT | 1 cut(s) | 352 |
| BseDI | CCNNGG | 5 cut(s) | 147, 188, 251, 313, 579 |
| BseGI | GGATG | 5 cut(s) | 143, 247, 345, 493, 793 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 127, 190, 523, 700 |
| BseMI | GCAATG | 1 cut(s) | 477 |
| BseNI | ACTGG | 2 cut(s) | 204, 309 |
| BseXI | GCAGC | 2 cut(s) | 448, 508 |
| BsgI | GTGCAG | 1 cut(s) | 30 |
| BshFI | GGCC | 1 cut(s) | 665 |
| BshVI | ATCGAT | 1 cut(s) | 352 |
| BslFI | GGGAC | 2 cut(s) | 308, 704 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 127, 190, 523, 700 |
| BsmFI | GGGAC | 2 cut(s) | 308, 704 |
| BsnI | GGCC | 1 cut(s) | 665 |
| Bsp119I | TTCGAA | 1 cut(s) | 455 |
| Bsp143I | GATC | 2 cut(s) | 151, 216 |
| Bsp19I | CCATGG | 1 cut(s) | 188 |
| BspACI | CCGC | 5 cut(s) | 97, 128, 661, 694, 736 |
| BspANI | GGCC | 1 cut(s) | 665 |
| BspDI | ATCGAT | 1 cut(s) | 352 |
| BspHI | TCATGA | 2 cut(s) | 382, 394 |
| BspLI | GGNNCC | 1 cut(s) | 692 |
| BspPI | GGATC | 1 cut(s) | 159 |
| BspT104I | TTCGAA | 1 cut(s) | 455 |
| BspTI | CTTAAG | 1 cut(s) | 680 |
| BsrDI | GCAATG | 1 cut(s) | 477 |
| BsrI | ACTGG | 2 cut(s) | 204, 309 |
| BssECI | CCNNGG | 5 cut(s) | 147, 188, 251, 313, 579 |
| BssMI | GATC | 2 cut(s) | 151, 216 |
| BssNAI | GTATAC | 1 cut(s) | 297 |
| BssT1I | CCWWGG | 2 cut(s) | 188, 251 |
| Bst1107I | GTATAC | 1 cut(s) | 297 |
| Bst2UI | CCWGG | 4 cut(s) | 140, 148, 314, 581 |
| Bst4CI | ACNGT | 4 cut(s) | 93, 116, 389, 648 |
| Bst6I | CTCTTC | 1 cut(s) | 633 |
| BstAFI | CTTAAG | 1 cut(s) | 680 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 53 |
| BstBI | TTCGAA | 1 cut(s) | 455 |
| BstC8I | GCNNGC | 2 cut(s) | 185, 663 |
| BstDSI | CCRYGG | 1 cut(s) | 188 |
| BstEII | GGTNACC | 1 cut(s) | 116 |
| BstF5I | GGATG | 5 cut(s) | 143, 247, 345, 493, 793 |
| BstKTI | GATC | 2 cut(s) | 154, 219 |
| BstMBI | GATC | 2 cut(s) | 151, 216 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 53, 488, 571 |
| BstNI | CCWGG | 4 cut(s) | 140, 148, 314, 581 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 253, 390 |
| BstPI | GGTNACC | 1 cut(s) | 116 |
| BstSCI | CCNGG | 4 cut(s) | 138, 146, 312, 579 |
| BstV1I | GCAGC | 2 cut(s) | 448, 508 |
| BstV2I | GAAGAC | 1 cut(s) | 499 |
| BstX2I | RGATCY | 2 cut(s) | 151, 216 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 93 |
| BstYI | RGATCY | 2 cut(s) | 151, 216 |
| BstZ17I | GTATAC | 1 cut(s) | 297 |
| Bsu15I | ATCGAT | 1 cut(s) | 352 |
| BsuRI | GGCC | 1 cut(s) | 665 |
| BsuTUI | ATCGAT | 1 cut(s) | 352 |
| BtgI | CCRYGG | 1 cut(s) | 188 |
| BtrI | CACGTC | 2 cut(s) | 7, 53 |
| BtsCI | GGATG | 5 cut(s) | 143, 247, 345, 493, 793 |
| Cac8I | GCNNGC | 2 cut(s) | 185, 663 |
| CciI | TCATGA | 2 cut(s) | 382, 394 |
| Cfr13I | GGNCC | 3 cut(s) | 514, 663, 691 |
| ClaI | ATCGAT | 1 cut(s) | 352 |
| Csp6I | GTAC | 3 cut(s) | 197, 545, 614 |
| CviAII | CATG | 5 cut(s) | 103, 189, 383, 395, 442 |
| CviQI | GTAC | 3 cut(s) | 197, 545, 614 |
| DpnI | GATC | 2 cut(s) | 153, 218 |
| DpnII | GATC | 2 cut(s) | 151, 216 |
| DraIII | CACNNNGTG | 1 cut(s) | 53 |
| Eam1104I | CTCTTC | 1 cut(s) | 633 |
| EarI | CTCTTC | 1 cut(s) | 633 |
| Eco130I | CCWWGG | 2 cut(s) | 188, 251 |
| Eco47I | GGWCC | 2 cut(s) | 514, 691 |
| Eco91I | GGTNACC | 1 cut(s) | 116 |
| EcoO65I | GGTNACC | 1 cut(s) | 116 |
| EcoRII | CCWGG | 4 cut(s) | 138, 146, 312, 579 |
| EcoT14I | CCWWGG | 2 cut(s) | 188, 251 |
| EcoT22I | ATGCAT | 1 cut(s) | 289 |
| ErhI | CCWWGG | 2 cut(s) | 188, 251 |
| FaeI | CATG | 5 cut(s) | 106, 192, 386, 398, 445 |
| FaqI | GGGAC | 2 cut(s) | 308, 704 |
| FatI | CATG | 5 cut(s) | 102, 188, 382, 394, 441 |
| FauI | CCCGC | 1 cut(s) | 654 |
| FblI | GTMKAC | 4 cut(s) | 247, 296, 309, 421 |
| Fnu4HI | GCNGC | 3 cut(s) | 129, 437, 497 |
| FokI | GGATG | 5 cut(s) | 130, 254, 352, 500, 800 |
| Fsp4HI | GCNGC | 3 cut(s) | 129, 437, 497 |
| FspBI | CTAG | 2 cut(s) | 474, 551 |
| GluI | GCNGC | 3 cut(s) | 129, 437, 497 |
| HaeIII | GGCC | 1 cut(s) | 665 |
| Hin1II | CATG | 5 cut(s) | 106, 192, 386, 398, 445 |
| HincII | GTYRAC | 1 cut(s) | 310 |
| HindII | GTYRAC | 1 cut(s) | 310 |
| HindIII | AAGCTT | 1 cut(s) | 669 |
| HinfI | GANTC | 5 cut(s) | 262, 354, 365, 655, 803 |
| HphI | GGTGA | 1 cut(s) | 215 |
| Hpy166II | GTNNAC | 4 cut(s) | 248, 297, 310, 422 |
| Hpy188I | TCNGA | 6 cut(s) | 232, 259, 281, 336, 637, 717 |
| Hpy188III | TCNNGA | 3 cut(s) | 16, 383, 395 |
| Hpy8I | GTNNAC | 4 cut(s) | 248, 297, 310, 422 |
| Hpy99I | CGWCG | 1 cut(s) | 54 |
| HpyAV | CCTTC | 3 cut(s) | 154, 680, 712 |
| HpyCH4III | ACNGT | 4 cut(s) | 93, 116, 389, 648 |
| HpyCH4IV | ACGT | 2 cut(s) | 6, 52 |
| HpyCH4V | TGCA | 3 cut(s) | 47, 287, 379 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 53, 488, 571 |
| HpySE526I | ACGT | 2 cut(s) | 6, 52 |
| Hsp92II | CATG | 5 cut(s) | 106, 192, 386, 398, 445 |
| Kzo9I | GATC | 2 cut(s) | 151, 216 |
| Lsp1109I | GCAGC | 2 cut(s) | 448, 508 |
| LweI | GCATC | 5 cut(s) | 152, 236, 274, 388, 478 |
| MaeI | CTAG | 2 cut(s) | 474, 551 |
| MaeII | ACGT | 2 cut(s) | 6, 52 |
| MaeIII | GTNAC | 2 cut(s) | 116, 203 |
| MalI | GATC | 2 cut(s) | 153, 218 |
| MboI | GATC | 2 cut(s) | 151, 216 |
| MboII | GAAGA | 4 cut(s) | 73, 469, 499, 650 |
| MfeI | CAATTG | 1 cut(s) | 617 |
| MflI | RGATCY | 2 cut(s) | 151, 216 |
| MluCI | AATT | 5 cut(s) | 19, 528, 605, 617, 817 |
| MlyI | GAGTC | 1 cut(s) | 664 |
| MmeI | TCCRAC | 4 cut(s) | 219, 237, 314, 465 |
| Mph1103I | ATGCAT | 1 cut(s) | 289 |
| MroXI | GAANNNNTTC | 1 cut(s) | 743 |
| MseI | TTAA | 3 cut(s) | 210, 608, 681 |
| MspA1I | CMGCKG | 1 cut(s) | 661 |
| MspCI | CTTAAG | 1 cut(s) | 680 |
| MspR9I | CCNGG | 4 cut(s) | 140, 148, 314, 581 |
| MunI | CAATTG | 1 cut(s) | 617 |
| MvaI | CCWGG | 4 cut(s) | 140, 148, 314, 581 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 53, 488, 571 |
| NcoI | CCATGG | 1 cut(s) | 188 |
| NdeII | GATC | 2 cut(s) | 151, 216 |
| NlaIII | CATG | 5 cut(s) | 106, 192, 386, 398, 445 |
| NlaIV | GGNNCC | 1 cut(s) | 692 |
| NmuCI | GTSAC | 1 cut(s) | 203 |
| NsiI | ATGCAT | 1 cut(s) | 289 |
| NspV | TTCGAA | 1 cut(s) | 455 |
| PagI | TCATGA | 2 cut(s) | 382, 394 |
| PdmI | GAANNNNTTC | 1 cut(s) | 743 |
| PfeI | GAWTC | 4 cut(s) | 262, 354, 365, 803 |
| PkrI | GCNGC | 3 cut(s) | 130, 438, 498 |
| PleI | GAGTC | 1 cut(s) | 663 |
| PpsI | GAGTC | 1 cut(s) | 663 |
| PshAI | GACNNNNGTC | 2 cut(s) | 253, 390 |
| PshBI | ATTAAT | 1 cut(s) | 608 |
| Psp6I | CCWGG | 4 cut(s) | 138, 146, 312, 579 |
| PspEI | GGTNACC | 1 cut(s) | 116 |
| PspGI | CCWGG | 4 cut(s) | 138, 146, 312, 579 |
| PspN4I | GGNNCC | 1 cut(s) | 692 |
| PspPI | GGNCC | 3 cut(s) | 514, 663, 691 |
| PsuI | RGATCY | 2 cut(s) | 151, 216 |
| RsaI | GTAC | 3 cut(s) | 198, 546, 615 |
| RsaNI | GTAC | 3 cut(s) | 197, 545, 614 |
| SalI | GTCGAC | 1 cut(s) | 308 |
| SaqAI | TTAA | 3 cut(s) | 210, 608, 681 |
| SatI | GCNGC | 3 cut(s) | 129, 437, 497 |
| Sau3AI | GATC | 2 cut(s) | 151, 216 |
| Sau96I | GGNCC | 3 cut(s) | 514, 663, 691 |
| SchI | GAGTC | 1 cut(s) | 664 |
| ScrFI | CCNGG | 4 cut(s) | 140, 148, 314, 581 |
| SfaNI | GCATC | 5 cut(s) | 152, 236, 274, 388, 478 |
| SfuI | TTCGAA | 1 cut(s) | 455 |
| SinI | GGWCC | 2 cut(s) | 514, 691 |
| SmlI | CTYRAG | 1 cut(s) | 680 |
| SmoI | CTYRAG | 1 cut(s) | 680 |
| Sse9I | AATT | 5 cut(s) | 19, 528, 605, 617, 817 |
| SsiI | CCGC | 5 cut(s) | 97, 128, 661, 694, 736 |
| SspMI | CTAG | 2 cut(s) | 474, 551 |
| StyD4I | CCNGG | 4 cut(s) | 138, 146, 312, 579 |
| StyI | CCWWGG | 2 cut(s) | 188, 251 |
| TaaI | ACNGT | 4 cut(s) | 93, 116, 389, 648 |
| TaiI | ACGT | 2 cut(s) | 9, 55 |
| TaqI | TCGA | 4 cut(s) | 25, 309, 352, 455 |
| TaqII | GACCGA | 1 cut(s) | 531 |
| TasI | AATT | 5 cut(s) | 19, 528, 605, 617, 817 |
| TatI | WGTACW | 1 cut(s) | 196 |
| TauI | GCSGC | 1 cut(s) | 131 |
| TfiI | GAWTC | 4 cut(s) | 262, 354, 365, 803 |
| Tru1I | TTAA | 3 cut(s) | 210, 608, 681 |
| Tru9I | TTAA | 3 cut(s) | 210, 608, 681 |
| TseFI | GTSAC | 1 cut(s) | 203 |
| TseI | GCWGC | 2 cut(s) | 436, 496 |
| Tsp45I | GTSAC | 1 cut(s) | 203 |
| TspDTI | ATGAA | 4 cut(s) | 91, 395, 701, 736 |
| Vha464I | CTTAAG | 1 cut(s) | 680 |
| VpaK11BI | GGWCC | 2 cut(s) | 514, 691 |
| VspI | ATTAAT | 1 cut(s) | 608 |
| XmiI | GTMKAC | 4 cut(s) | 247, 296, 309, 421 |
| XmnI | GAANNNNTTC | 1 cut(s) | 743 |
| XspI | CTAG | 2 cut(s) | 474, 551 |
| Zsp2I | ATGCAT | 1 cut(s) | 289 |