ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 102 |
| Acc65I | GGTACC | 1 cut(s) | 538 |
| AccB1I | GGYRCC | 2 cut(s) | 490, 538 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 500, 650 |
| AccBSI | CCGCTC | 1 cut(s) | 90 |
| AccI | GTMKAC | 1 cut(s) | 905 |
| AccII | CGCG | 1 cut(s) | 475 |
| AciI | CCGC | 4 cut(s) | 47, 88, 470, 965 |
| AclI | AACGTT | 2 cut(s) | 717, 778 |
| AclWI | GGATC | 1 cut(s) | 216 |
| AcoI | YGGCCR | 2 cut(s) | 602, 976 |
| AcsI | RAATTY | 2 cut(s) | 727, 760 |
| AcyI | GRCGYC | 1 cut(s) | 772 |
| AfaI | GTAC | 4 cut(s) | 540, 824, 1052, 1091 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 436, 500, 650 |
| AgsI | TTSAA | 4 cut(s) | 61, 400, 702, 843 |
| AjnI | CCWGG | 3 cut(s) | 222, 534, 649 |
| AleI | CACNNNNGTG | 1 cut(s) | 1058 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 1064 |
| AluBI | AGCT | 7 cut(s) | 274, 567, 588, 736, 839, 1000, 1022 |
| AluI | AGCT | 7 cut(s) | 274, 567, 588, 736, 839, 1000, 1022 |
| Alw21I | GWGCWC | 3 cut(s) | 933, 1002, 1048 |
| Alw26I | GTCTC | 1 cut(s) | 801 |
| Alw44I | GTGCAC | 1 cut(s) | 1044 |
| AlwI | GGATC | 1 cut(s) | 216 |
| Ama87I | CYCGRG | 1 cut(s) | 406 |
| AoxI | GGCC | 3 cut(s) | 403, 602, 976 |
| ApaI | GGGCCC | 1 cut(s) | 407 |
| ApaLI | GTGCAC | 1 cut(s) | 1044 |
| ApeKI | GCWGC | 5 cut(s) | 44, 214, 284, 588, 1006 |
| ApoI | RAATTY | 2 cut(s) | 727, 760 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 824 |
| Asp718I | GGTACC | 1 cut(s) | 538 |
| AspA2I | CCTAGG | 1 cut(s) | 1010 |
| AspLEI | GCGC | 2 cut(s) | 103, 475 |
| AspS9I | GGNCC | 5 cut(s) | 307, 403, 404, 427, 453 |
| AsuC2I | CCSGG | 4 cut(s) | 26, 407, 408, 601 |
| AsuNHI | GCTAGC | 1 cut(s) | 1022 |
| AvaI | CYCGRG | 1 cut(s) | 406 |
| AvaII | GGWCC | 3 cut(s) | 307, 427, 453 |
| AvrII | CCTAGG | 1 cut(s) | 1010 |
| BaeGI | GKGCMC | 2 cut(s) | 407, 1048 |
| BalI | TGGCCA | 1 cut(s) | 978 |
| BanI | GGYRCC | 2 cut(s) | 490, 538 |
| BanII | GRGCYC | 2 cut(s) | 407, 1002 |
| BbsI | GAAGAC | 1 cut(s) | 228 |
| Bbv12I | GWGCWC | 3 cut(s) | 933, 1002, 1048 |
| BbvI | GCAGC | 5 cut(s) | 56, 201, 296, 575, 1018 |
| BccI | CCATC | 2 cut(s) | 128, 910 |
| BceAI | ACGGC | 3 cut(s) | 201, 419, 459 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 551, 585 |
| BciT130I | CCWGG | 3 cut(s) | 224, 536, 651 |
| BclI | TGATCA | 1 cut(s) | 880 |
| BcnI | CCSGG | 4 cut(s) | 26, 407, 408, 601 |
| BcoDI | GTCTC | 1 cut(s) | 801 |
| BfaI | CTAG | 3 cut(s) | 749, 1011, 1023 |
| BfmI | CTRYAG | 1 cut(s) | 957 |
| BisI | GCNGC | 6 cut(s) | 45, 215, 285, 471, 589, 1007 |
| BlnI | CCTAGG | 1 cut(s) | 1010 |
| BlsI | GCNGC | 6 cut(s) | 46, 216, 286, 472, 590, 1008 |
| BmcAI | AGTACT | 1 cut(s) | 824 |
| Bme1390I | CCNGG | 7 cut(s) | 26, 224, 407, 408, 536, 601, 651 |
| Bme18I | GGWCC | 3 cut(s) | 307, 427, 453 |
| BmeT110I | CYCGRG | 1 cut(s) | 406 |
| BmgT120I | GGNCC | 5 cut(s) | 307, 403, 404, 427, 453 |
| BmiI | GGNNCC | 3 cut(s) | 405, 492, 540 |
| BmrFI | CCNGG | 7 cut(s) | 26, 224, 407, 408, 536, 601, 651 |
| BmsI | GCATC | 6 cut(s) | 112, 184, 315, 730, 952, 1063 |
| BmtI | GCTAGC | 1 cut(s) | 1026 |
| BpiI | GAAGAC | 1 cut(s) | 228 |
| BpmI | CTGGAG | 1 cut(s) | 672 |
| BpuMI | CCSGG | 4 cut(s) | 26, 407, 408, 601 |
| BsaAI | YACGTR | 1 cut(s) | 450 |
| BsaHI | GRCGYC | 1 cut(s) | 772 |
| BsaJI | CCNNGG | 8 cut(s) | 25, 222, 245, 406, 435, 499, 649, 1010 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 291, 321, 370, 400 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 436, 500, 650 |
| Bse1I | ACTGG | 3 cut(s) | 166, 179, 385 |
| BseBI | CCWGG | 3 cut(s) | 224, 536, 651 |
| BseDI | CCNNGG | 8 cut(s) | 25, 222, 245, 406, 435, 499, 649, 1010 |
| BseGI | GGATG | 5 cut(s) | 73, 175, 745, 921, 1078 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 436, 500, 650 |
| BseMII | CTCAG | 1 cut(s) | 222 |
| BseNI | ACTGG | 3 cut(s) | 166, 179, 385 |
| BseRI | GAGGAG | 1 cut(s) | 430 |
| BseSI | GKGCMC | 2 cut(s) | 407, 1048 |
| BseXI | GCAGC | 5 cut(s) | 56, 201, 296, 575, 1018 |
| BseYI | CCCAGC | 1 cut(s) | 633 |
| BsgI | GTGCAG | 1 cut(s) | 644 |
| Bsh1236I | CGCG | 1 cut(s) | 475 |
| BshFI | GGCC | 3 cut(s) | 405, 604, 978 |
| BshNI | GGYRCC | 2 cut(s) | 490, 538 |
| BsiHKAI | GWGCWC | 3 cut(s) | 933, 1002, 1048 |
| BsiHKCI | CYCGRG | 1 cut(s) | 406 |
| BsiSI | CCGG | 5 cut(s) | 25, 41, 310, 407, 601 |
| BslFI | GGGAC | 2 cut(s) | 205, 650 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 436, 500, 650 |
| BsmAI | GTCTC | 1 cut(s) | 801 |
| BsmFI | GGGAC | 2 cut(s) | 205, 650 |
| BsmI | GAATGC | 1 cut(s) | 1036 |
| BsnI | GGCC | 3 cut(s) | 405, 604, 978 |
| BsoBI | CYCGRG | 1 cut(s) | 406 |
| Bsp120I | GGGCCC | 1 cut(s) | 403 |
| Bsp1286I | GDGCHC | 4 cut(s) | 407, 933, 1002, 1048 |
| Bsp143I | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| Bsp19I | CCATGG | 1 cut(s) | 499 |
| BspACI | CCGC | 4 cut(s) | 47, 88, 470, 965 |
| BspANI | GGCC | 3 cut(s) | 405, 604, 978 |
| BspCNI | CTCAG | 1 cut(s) | 223 |
| BspFNI | CGCG | 1 cut(s) | 475 |
| BspLI | GGNNCC | 3 cut(s) | 405, 492, 540 |
| BspMAI | CTGCAG | 1 cut(s) | 961 |
| BspOI | GCTAGC | 1 cut(s) | 1026 |
| BspPI | GGATC | 1 cut(s) | 216 |
| BspQI | GCTCTTC | 1 cut(s) | 844 |
| BspT107I | GGYRCC | 2 cut(s) | 490, 538 |
| BsrBI | CCGCTC | 1 cut(s) | 90 |
| BsrI | ACTGG | 3 cut(s) | 166, 179, 385 |
| BssECI | CCNNGG | 8 cut(s) | 25, 222, 245, 406, 435, 499, 649, 1010 |
| BssMI | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| BssNI | GRCGYC | 1 cut(s) | 772 |
| BssT1I | CCWWGG | 2 cut(s) | 499, 1010 |
| Bst2UI | CCWGG | 3 cut(s) | 224, 536, 651 |
| Bst4CI | ACNGT | 7 cut(s) | 246, 320, 333, 431, 890, 1050, 1057 |
| Bst6I | CTCTTC | 2 cut(s) | 56, 844 |
| BstACI | GRCGYC | 1 cut(s) | 772 |
| BstBAI | YACGTR | 1 cut(s) | 450 |
| BstC8I | GCNNGC | 1 cut(s) | 1024 |
| BstDEI | CTNAG | 4 cut(s) | 93, 231, 370, 523 |
| BstDSI | CCRYGG | 3 cut(s) | 245, 435, 499 |
| BstF5I | GGATG | 5 cut(s) | 73, 175, 745, 921, 1078 |
| BstFNI | CGCG | 1 cut(s) | 475 |
| BstHHI | GCGC | 2 cut(s) | 103, 475 |
| BstKTI | GATC | 6 cut(s) | 211, 258, 361, 508, 685, 883 |
| BstMAI | GTCTC | 1 cut(s) | 801 |
| BstMBI | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 53, 585, 1006 |
| BstNI | CCWGG | 3 cut(s) | 224, 536, 651 |
| BstSCI | CCNGG | 7 cut(s) | 24, 222, 405, 406, 534, 599, 649 |
| BstSFI | CTRYAG | 1 cut(s) | 957 |
| BstSLI | GKGCMC | 2 cut(s) | 407, 1048 |
| BstUI | CGCG | 1 cut(s) | 475 |
| BstV1I | GCAGC | 5 cut(s) | 56, 201, 296, 575, 1018 |
| BstV2I | GAAGAC | 1 cut(s) | 228 |
| BstX2I | RGATCY | 1 cut(s) | 208 |
| BstYI | RGATCY | 1 cut(s) | 208 |
| BsuRI | GGCC | 3 cut(s) | 405, 604, 978 |
| BtgI | CCRYGG | 3 cut(s) | 245, 435, 499 |
| BtsCI | GGATG | 5 cut(s) | 73, 175, 745, 921, 1078 |
| BtsI | GCAGTG | 1 cut(s) | 954 |
| BtsIMutI | CAGTG | 3 cut(s) | 129, 186, 954 |
| Cac8I | GCNNGC | 1 cut(s) | 1024 |
| CfoI | GCGC | 2 cut(s) | 103, 475 |
| Cfr13I | GGNCC | 5 cut(s) | 307, 403, 404, 427, 453 |
| Cfr9I | CCCGGG | 1 cut(s) | 406 |
| CseI | GACGC | 2 cut(s) | 536, 761 |
| Csp6I | GTAC | 4 cut(s) | 539, 823, 1051, 1090 |
| CviAII | CATG | 5 cut(s) | 204, 281, 500, 664, 1027 |
| CviQI | GTAC | 4 cut(s) | 539, 823, 1051, 1090 |
| DdeI | CTNAG | 4 cut(s) | 93, 231, 370, 523 |
| DpnI | GATC | 6 cut(s) | 210, 257, 360, 507, 684, 882 |
| DpnII | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| EaeI | YGGCCR | 2 cut(s) | 602, 976 |
| Eam1104I | CTCTTC | 2 cut(s) | 56, 844 |
| EarI | CTCTTC | 2 cut(s) | 56, 844 |
| Ecl136II | GAGCTC | 1 cut(s) | 1000 |
| Eco130I | CCWWGG | 2 cut(s) | 499, 1010 |
| Eco24I | GRGCYC | 2 cut(s) | 407, 1002 |
| Eco47I | GGWCC | 3 cut(s) | 307, 427, 453 |
| Eco53kI | GAGCTC | 1 cut(s) | 1000 |
| Eco88I | CYCGRG | 1 cut(s) | 406 |
| EcoICRI | GAGCTC | 1 cut(s) | 1000 |
| EcoO109I | RGGNCCY | 1 cut(s) | 403 |
| EcoRI | GAATTC | 1 cut(s) | 727 |
| EcoRII | CCWGG | 3 cut(s) | 222, 534, 649 |
| EcoT14I | CCWWGG | 2 cut(s) | 499, 1010 |
| EcoT38I | GRGCYC | 2 cut(s) | 407, 1002 |
| ErhI | CCWWGG | 2 cut(s) | 499, 1010 |
| FaeI | CATG | 5 cut(s) | 207, 284, 503, 667, 1030 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1061, 1093 |
| FaqI | GGGAC | 2 cut(s) | 205, 650 |
| FatI | CATG | 5 cut(s) | 203, 280, 499, 663, 1026 |
| FauNDI | CATATG | 1 cut(s) | 981 |
| FbaI | TGATCA | 1 cut(s) | 880 |
| FblI | GTMKAC | 1 cut(s) | 905 |
| Fnu4HI | GCNGC | 6 cut(s) | 45, 215, 285, 471, 589, 1007 |
| FokI | GGATG | 5 cut(s) | 80, 162, 752, 928, 1085 |
| FriOI | GRGCYC | 2 cut(s) | 407, 1002 |
| Fsp4HI | GCNGC | 6 cut(s) | 45, 215, 285, 471, 589, 1007 |
| FspBI | CTAG | 3 cut(s) | 749, 1011, 1023 |
| FspI | TGCGCA | 1 cut(s) | 102 |
| GlaI | GCGC | 2 cut(s) | 102, 474 |
| GluI | GCNGC | 6 cut(s) | 45, 215, 285, 471, 589, 1007 |
| GsaI | CCCAGC | 1 cut(s) | 637 |
| GsuI | CTGGAG | 1 cut(s) | 672 |
| HaeIII | GGCC | 3 cut(s) | 405, 604, 978 |
| HapII | CCGG | 5 cut(s) | 25, 41, 310, 407, 601 |
| HgaI | GACGC | 2 cut(s) | 536, 761 |
| HhaI | GCGC | 2 cut(s) | 103, 475 |
| Hin1I | GRCGYC | 1 cut(s) | 772 |
| Hin1II | CATG | 5 cut(s) | 207, 284, 503, 667, 1030 |
| Hin6I | GCGC | 2 cut(s) | 101, 473 |
| HinP1I | GCGC | 2 cut(s) | 101, 473 |
| HincII | GTYRAC | 2 cut(s) | 776, 906 |
| HindII | GTYRAC | 2 cut(s) | 776, 906 |
| HindIII | AAGCTT | 1 cut(s) | 837 |
| HinfI | GANTC | 5 cut(s) | 233, 698, 859, 902, 920 |
| HpaII | CCGG | 5 cut(s) | 25, 41, 310, 407, 601 |
| Hpy166II | GTNNAC | 7 cut(s) | 82, 378, 776, 906, 954, 1046, 1053 |
| Hpy188I | TCNGA | 4 cut(s) | 324, 338, 457, 672 |
| Hpy188III | TCNNGA | 2 cut(s) | 514, 1084 |
| Hpy8I | GTNNAC | 7 cut(s) | 82, 378, 776, 906, 954, 1046, 1053 |
| Hpy99I | CGWCG | 1 cut(s) | 552 |
| HpyAV | CCTTC | 4 cut(s) | 286, 290, 394, 905 |
| HpyCH4III | ACNGT | 7 cut(s) | 246, 320, 333, 431, 890, 1050, 1057 |
| HpyCH4IV | ACGT | 3 cut(s) | 449, 717, 778 |
| HpyCH4V | TGCA | 9 cut(s) | 284, 328, 557, 625, 868, 959, 985, 1006, 1046 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 53, 585, 1006 |
| HpyF3I | CTNAG | 4 cut(s) | 93, 231, 370, 523 |
| HpySE526I | ACGT | 3 cut(s) | 449, 717, 778 |
| Hsp92I | GRCGYC | 1 cut(s) | 772 |
| Hsp92II | CATG | 5 cut(s) | 207, 284, 503, 667, 1030 |
| HspAI | GCGC | 2 cut(s) | 101, 473 |
| KpnI | GGTACC | 1 cut(s) | 542 |
| Ksp22I | TGATCA | 1 cut(s) | 880 |
| Kzo9I | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| LguI | GCTCTTC | 1 cut(s) | 844 |
| LmnI | GCTCC | 1 cut(s) | 936 |
| Lsp1109I | GCAGC | 5 cut(s) | 56, 201, 296, 575, 1018 |
| LweI | GCATC | 6 cut(s) | 112, 184, 315, 730, 952, 1063 |
| MaeI | CTAG | 3 cut(s) | 749, 1011, 1023 |
| MaeII | ACGT | 3 cut(s) | 449, 717, 778 |
| MaeIII | GTNAC | 5 cut(s) | 181, 314, 744, 787, 1015 |
| MalI | GATC | 6 cut(s) | 210, 257, 360, 507, 684, 882 |
| MbiI | CCGCTC | 1 cut(s) | 90 |
| MboI | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| MboII | GAAGA | 6 cut(s) | 73, 228, 473, 764, 767, 861 |
| MflI | RGATCY | 1 cut(s) | 208 |
| MhlI | GDGCHC | 4 cut(s) | 407, 933, 1002, 1048 |
| MlsI | TGGCCA | 1 cut(s) | 978 |
| MluCI | AATT | 3 cut(s) | 19, 727, 760 |
| MluNI | TGGCCA | 1 cut(s) | 978 |
| MlyI | GAGTC | 3 cut(s) | 242, 911, 929 |
| MmeI | TCCRAC | 1 cut(s) | 480 |
| MnlI | CCTC | 9 cut(s) | 46, 57, 64, 147, 293, 408, 622, 647, 796 |
| Mox20I | TGGCCA | 1 cut(s) | 978 |
| MroXI | GAANNNNTTC | 1 cut(s) | 824 |
| MscI | TGGCCA | 1 cut(s) | 978 |
| MseI | TTAA | 1 cut(s) | 1068 |
| MslI | CAYNNNNRTG | 3 cut(s) | 111, 464, 1058 |
| Msp20I | TGGCCA | 1 cut(s) | 978 |
| MspA1I | CMGCKG | 1 cut(s) | 47 |
| MspI | CCGG | 5 cut(s) | 25, 41, 310, 407, 601 |
| MspR9I | CCNGG | 7 cut(s) | 26, 224, 407, 408, 536, 601, 651 |
| Mva1269I | GAATGC | 1 cut(s) | 1036 |
| MvaI | CCWGG | 3 cut(s) | 224, 536, 651 |
| MvnI | CGCG | 1 cut(s) | 475 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 53, 585, 1006 |
| NciI | CCSGG | 4 cut(s) | 26, 407, 408, 601 |
| NcoI | CCATGG | 1 cut(s) | 499 |
| NdeI | CATATG | 1 cut(s) | 981 |
| NdeII | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| NheI | GCTAGC | 1 cut(s) | 1022 |
| NlaIII | CATG | 5 cut(s) | 207, 284, 503, 667, 1030 |
| NlaIV | GGNNCC | 3 cut(s) | 405, 492, 540 |
| NmuCI | GTSAC | 4 cut(s) | 181, 314, 787, 1015 |
| NsbI | TGCGCA | 1 cut(s) | 102 |
| OliI | CACNNNNGTG | 1 cut(s) | 1058 |
| PciSI | GCTCTTC | 1 cut(s) | 844 |
| PctI | GAATGC | 1 cut(s) | 1036 |
| PdmI | GAANNNNTTC | 1 cut(s) | 824 |
| PfeI | GAWTC | 2 cut(s) | 698, 859 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 500, 650 |
| PkrI | GCNGC | 6 cut(s) | 46, 216, 286, 472, 590, 1008 |
| PleI | GAGTC | 3 cut(s) | 241, 910, 928 |
| PpsI | GAGTC | 3 cut(s) | 241, 910, 928 |
| Ppu21I | YACGTR | 1 cut(s) | 450 |
| Psp124BI | GAGCTC | 1 cut(s) | 1002 |
| Psp1406I | AACGTT | 2 cut(s) | 717, 778 |
| Psp6I | CCWGG | 3 cut(s) | 222, 534, 649 |
| PspFI | CCCAGC | 1 cut(s) | 633 |
| PspGI | CCWGG | 3 cut(s) | 222, 534, 649 |
| PspN4I | GGNNCC | 3 cut(s) | 405, 492, 540 |
| PspOMI | GGGCCC | 1 cut(s) | 403 |
| PspPI | GGNCC | 5 cut(s) | 307, 403, 404, 427, 453 |
| PstI | CTGCAG | 1 cut(s) | 961 |
| PsuI | RGATCY | 1 cut(s) | 208 |
| RsaI | GTAC | 4 cut(s) | 540, 824, 1052, 1091 |
| RsaNI | GTAC | 4 cut(s) | 539, 823, 1051, 1090 |
| RseI | CAYNNNNRTG | 3 cut(s) | 111, 464, 1058 |
| SacI | GAGCTC | 1 cut(s) | 1002 |
| SalI | GTCGAC | 1 cut(s) | 904 |
| SapI | GCTCTTC | 1 cut(s) | 844 |
| SaqAI | TTAA | 1 cut(s) | 1068 |
| SatI | GCNGC | 6 cut(s) | 45, 215, 285, 471, 589, 1007 |
| Sau3AI | GATC | 6 cut(s) | 208, 255, 358, 505, 682, 880 |
| Sau96I | GGNCC | 5 cut(s) | 307, 403, 404, 427, 453 |
| ScaI | AGTACT | 1 cut(s) | 824 |
| SchI | GAGTC | 3 cut(s) | 242, 911, 929 |
| ScrFI | CCNGG | 7 cut(s) | 26, 224, 407, 408, 536, 601, 651 |
| SduI | GDGCHC | 4 cut(s) | 407, 933, 1002, 1048 |
| SfaNI | GCATC | 6 cut(s) | 112, 184, 315, 730, 952, 1063 |
| SfcI | CTRYAG | 1 cut(s) | 957 |
| SinI | GGWCC | 3 cut(s) | 307, 427, 453 |
| SmaI | CCCGGG | 1 cut(s) | 408 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 111, 464, 1058 |
| Sse9I | AATT | 3 cut(s) | 19, 727, 760 |
| SsiI | CCGC | 4 cut(s) | 47, 88, 470, 965 |
| SspMI | CTAG | 3 cut(s) | 749, 1011, 1023 |
| SstI | GAGCTC | 1 cut(s) | 1002 |
| StyD4I | CCNGG | 7 cut(s) | 24, 222, 405, 406, 534, 599, 649 |
| StyI | CCWWGG | 2 cut(s) | 499, 1010 |
| TaaI | ACNGT | 7 cut(s) | 246, 320, 333, 431, 890, 1050, 1057 |
| TaiI | ACGT | 3 cut(s) | 452, 720, 781 |
| TaqI | TCGA | 3 cut(s) | 550, 681, 905 |
| TasI | AATT | 3 cut(s) | 19, 727, 760 |
| TatI | WGTACW | 2 cut(s) | 822, 1089 |
| TauI | GCSGC | 1 cut(s) | 473 |
| TfiI | GAWTC | 2 cut(s) | 698, 859 |
| Tru1I | TTAA | 1 cut(s) | 1068 |
| Tru9I | TTAA | 1 cut(s) | 1068 |
| TscAI | CASTG | 3 cut(s) | 136, 186, 961 |
| TseFI | GTSAC | 4 cut(s) | 181, 314, 787, 1015 |
| TseI | GCWGC | 5 cut(s) | 44, 214, 284, 588, 1006 |
| Tsp45I | GTSAC | 4 cut(s) | 181, 314, 787, 1015 |
| TspDTI | ATGAA | 4 cut(s) | 269, 281, 473, 1043 |
| TspMI | CCCGGG | 1 cut(s) | 406 |
| TspRI | CASTG | 3 cut(s) | 136, 186, 961 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 500, 650 |
| VneI | GTGCAC | 1 cut(s) | 1044 |
| VpaK11BI | GGWCC | 3 cut(s) | 307, 427, 453 |
| XapI | RAATTY | 2 cut(s) | 727, 760 |
| XmaI | CCCGGG | 1 cut(s) | 406 |
| XmaJI | CCTAGG | 1 cut(s) | 1010 |
| XmiI | GTMKAC | 1 cut(s) | 905 |
| XmnI | GAANNNNTTC | 1 cut(s) | 824 |
| XspI | CTAG | 3 cut(s) | 749, 1011, 1023 |
| ZrmI | AGTACT | 1 cut(s) | 824 |