ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 955, 1043 |
| AccI | GTMKAC | 1 cut(s) | 892 |
| AccII | CGCG | 2 cut(s) | 47, 850 |
| AccIII | TCCGGA | 1 cut(s) | 124 |
| AciI | CCGC | 2 cut(s) | 45, 47 |
| AclWI | GGATC | 1 cut(s) | 116 |
| AcuI | CTGAAG | 1 cut(s) | 963 |
| AcyI | GRCGYC | 1 cut(s) | 602 |
| AdeI | CACNNNGTG | 1 cut(s) | 344 |
| AfaI | GTAC | 3 cut(s) | 131, 465, 689 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 558, 656, 682, 973 |
| AflIII | ACRYGT | 1 cut(s) | 848 |
| AgsI | TTSAA | 3 cut(s) | 61, 275, 713 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 988 |
| AjnI | CCWGG | 5 cut(s) | 359, 551, 605, 927, 971 |
| Alw21I | GWGCWC | 1 cut(s) | 381 |
| Alw26I | GTCTC | 5 cut(s) | 101, 121, 139, 304, 699 |
| AlwI | GGATC | 1 cut(s) | 116 |
| Ama87I | CYCGRG | 1 cut(s) | 630 |
| Aor13HI | TCCGGA | 1 cut(s) | 124 |
| AoxI | GGCC | 1 cut(s) | 190 |
| ApeKI | GCWGC | 1 cut(s) | 626 |
| AspLEI | GCGC | 1 cut(s) | 397 |
| AspS9I | GGNCC | 2 cut(s) | 190, 357 |
| AsuC2I | CCSGG | 1 cut(s) | 188 |
| AsuHPI | GGTGA | 4 cut(s) | 64, 344, 428, 671 |
| AvaI | CYCGRG | 1 cut(s) | 630 |
| AvaII | GGWCC | 1 cut(s) | 357 |
| BanII | GRGCYC | 1 cut(s) | 381 |
| Bbv12I | GWGCWC | 1 cut(s) | 381 |
| BbvI | GCAGC | 1 cut(s) | 613 |
| BccI | CCATC | 4 cut(s) | 94, 218, 284, 680 |
| BceAI | ACGGC | 1 cut(s) | 615 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 473, 507, 1017, 1051 |
| BciT130I | CCWGG | 5 cut(s) | 361, 553, 607, 929, 973 |
| BclI | TGATCA | 2 cut(s) | 69, 661 |
| BcnI | CCSGG | 1 cut(s) | 188 |
| BcoDI | GTCTC | 5 cut(s) | 101, 121, 139, 304, 699 |
| BfaI | CTAG | 4 cut(s) | 11, 155, 581, 701 |
| BfuAI | ACCTGC | 2 cut(s) | 955, 1043 |
| BisI | GCNGC | 1 cut(s) | 627 |
| BlsI | GCNGC | 1 cut(s) | 628 |
| Bme1390I | CCNGG | 6 cut(s) | 188, 361, 553, 607, 929, 973 |
| Bme18I | GGWCC | 1 cut(s) | 357 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 988 |
| BmeT110I | CYCGRG | 1 cut(s) | 630 |
| BmgT120I | GGNCC | 2 cut(s) | 190, 357 |
| BmiI | GGNNCC | 2 cut(s) | 191, 809 |
| BmrFI | CCNGG | 6 cut(s) | 188, 361, 553, 607, 929, 973 |
| BmrI | ACTGGG | 1 cut(s) | 336 |
| BmsI | GCATC | 4 cut(s) | 265, 471, 487, 563 |
| BmuI | ACTGGG | 1 cut(s) | 336 |
| BoxI | GACNNNNGTC | 1 cut(s) | 113 |
| BpmI | CTGGAG | 3 cut(s) | 170, 574, 911 |
| BpuMI | CCSGG | 1 cut(s) | 188 |
| BsaHI | GRCGYC | 1 cut(s) | 602 |
| BsaI | GGTCTC | 1 cut(s) | 121 |
| BsaJI | CCNNGG | 6 cut(s) | 45, 187, 401, 803, 971, 972 |
| BsaWI | WCCGGW | 3 cut(s) | 124, 197, 354 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 558, 656, 682, 973 |
| Bse118I | RCCGGY | 1 cut(s) | 576 |
| Bse1I | ACTGG | 3 cut(s) | 153, 193, 342 |
| BseAI | TCCGGA | 1 cut(s) | 124 |
| BseBI | CCWGG | 5 cut(s) | 361, 553, 607, 929, 973 |
| BseDI | CCNNGG | 6 cut(s) | 45, 187, 401, 803, 971, 972 |
| BseGI | GGATG | 2 cut(s) | 672, 893 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 558, 656, 682, 973 |
| BseMII | CTCAG | 2 cut(s) | 134, 549 |
| BseNI | ACTGG | 3 cut(s) | 153, 193, 342 |
| BseRI | GAGGAG | 1 cut(s) | 956 |
| BseXI | GCAGC | 1 cut(s) | 613 |
| BseYI | CCCAGC | 2 cut(s) | 452, 622 |
| BsgI | GTGCAG | 1 cut(s) | 789 |
| Bsh1236I | CGCG | 2 cut(s) | 47, 850 |
| BshFI | GGCC | 1 cut(s) | 192 |
| BsiHKAI | GWGCWC | 1 cut(s) | 381 |
| BsiHKCI | CYCGRG | 1 cut(s) | 630 |
| BsiSI | CCGG | 5 cut(s) | 125, 187, 198, 355, 577 |
| BslFI | GGGAC | 2 cut(s) | 610, 805 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 558, 656, 682, 973 |
| BsmAI | GTCTC | 5 cut(s) | 101, 121, 139, 304, 699 |
| BsmBI | CGTCTC | 1 cut(s) | 699 |
| BsmFI | GGGAC | 2 cut(s) | 610, 805 |
| BsnI | GGCC | 1 cut(s) | 192 |
| Bso31I | GGTCTC | 1 cut(s) | 121 |
| BsoBI | CYCGRG | 1 cut(s) | 630 |
| Bsp1286I | GDGCHC | 1 cut(s) | 381 |
| Bsp13I | TCCGGA | 1 cut(s) | 124 |
| Bsp143I | GATC | 4 cut(s) | 69, 121, 523, 661 |
| Bsp19I | CCATGG | 1 cut(s) | 401 |
| BspACI | CCGC | 2 cut(s) | 45, 47 |
| BspANI | GGCC | 1 cut(s) | 192 |
| BspCNI | CTCAG | 2 cut(s) | 135, 548 |
| BspEI | TCCGGA | 1 cut(s) | 124 |
| BspFNI | CGCG | 2 cut(s) | 47, 850 |
| BspLI | GGNNCC | 2 cut(s) | 191, 809 |
| BspMI | ACCTGC | 2 cut(s) | 955, 1043 |
| BspPI | GGATC | 1 cut(s) | 116 |
| BspQI | GCTCTTC | 1 cut(s) | 939 |
| BspTNI | GGTCTC | 1 cut(s) | 121 |
| BsrFI | RCCGGY | 1 cut(s) | 576 |
| BsrI | ACTGG | 3 cut(s) | 153, 193, 342 |
| BssAI | RCCGGY | 1 cut(s) | 576 |
| BssECI | CCNNGG | 6 cut(s) | 45, 187, 401, 803, 971, 972 |
| BssMI | GATC | 4 cut(s) | 69, 121, 523, 661 |
| BssNI | GRCGYC | 1 cut(s) | 602 |
| BssT1I | CCWWGG | 1 cut(s) | 401 |
| Bst2UI | CCWGG | 5 cut(s) | 361, 553, 607, 929, 973 |
| Bst4CI | ACNGT | 3 cut(s) | 170, 489, 709 |
| Bst6I | CTCTTC | 1 cut(s) | 939 |
| BstACI | GRCGYC | 1 cut(s) | 602 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 573 |
| BstC8I | GCNNGC | 4 cut(s) | 54, 565, 578, 1052 |
| BstDEI | CTNAG | 2 cut(s) | 143, 535 |
| BstDSI | CCRYGG | 3 cut(s) | 45, 401, 803 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 556 |
| BstF5I | GGATG | 2 cut(s) | 672, 893 |
| BstFNI | CGCG | 2 cut(s) | 47, 850 |
| BstHHI | GCGC | 1 cut(s) | 397 |
| BstKTI | GATC | 4 cut(s) | 72, 124, 526, 664 |
| BstMAI | GTCTC | 5 cut(s) | 101, 121, 139, 304, 699 |
| BstMBI | GATC | 4 cut(s) | 69, 121, 523, 661 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 53, 385, 394, 449, 573, 1028 |
| BstNI | CCWGG | 5 cut(s) | 361, 553, 607, 929, 973 |
| BstNSI | RCATGY | 2 cut(s) | 440, 1054 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 113 |
| BstSCI | CCNGG | 6 cut(s) | 186, 359, 551, 605, 927, 971 |
| BstUI | CGCG | 2 cut(s) | 47, 850 |
| BstV1I | GCAGC | 1 cut(s) | 613 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 200, 679 |
| BsuRI | GGCC | 1 cut(s) | 192 |
| BtgI | CCRYGG | 3 cut(s) | 45, 401, 803 |
| BtsCI | GGATG | 2 cut(s) | 672, 893 |
| BtsI | GCAGTG | 1 cut(s) | 597 |
| BtsIMutI | CAGTG | 3 cut(s) | 349, 597, 705 |
| BveI | ACCTGC | 2 cut(s) | 955, 1043 |
| Cac8I | GCNNGC | 4 cut(s) | 54, 565, 578, 1052 |
| CfoI | GCGC | 1 cut(s) | 397 |
| Cfr10I | RCCGGY | 1 cut(s) | 576 |
| Cfr13I | GGNCC | 2 cut(s) | 190, 357 |
| Cfr42I | CCGCGG | 1 cut(s) | 48 |
| CseI | GACGC | 4 cut(s) | 338, 373, 591, 903 |
| Csp6I | GTAC | 3 cut(s) | 130, 464, 688 |
| CviAII | CATG | 7 cut(s) | 133, 402, 437, 656, 665, 758, 1051 |
| CviQI | GTAC | 3 cut(s) | 130, 464, 688 |
| DdeI | CTNAG | 2 cut(s) | 143, 535 |
| DpnI | GATC | 4 cut(s) | 71, 123, 525, 663 |
| DpnII | GATC | 4 cut(s) | 69, 121, 523, 661 |
| DraIII | CACNNNGTG | 1 cut(s) | 344 |
| DriI | GACNNNNNGTC | 1 cut(s) | 988 |
| Eam1104I | CTCTTC | 1 cut(s) | 939 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 988 |
| EarI | CTCTTC | 1 cut(s) | 939 |
| Ecl136II | GAGCTC | 1 cut(s) | 379 |
| Eco130I | CCWWGG | 1 cut(s) | 401 |
| Eco24I | GRGCYC | 1 cut(s) | 381 |
| Eco31I | GGTCTC | 1 cut(s) | 121 |
| Eco47I | GGWCC | 1 cut(s) | 357 |
| Eco53kI | GAGCTC | 1 cut(s) | 379 |
| Eco57I | CTGAAG | 1 cut(s) | 963 |
| Eco88I | CYCGRG | 1 cut(s) | 630 |
| EcoICRI | GAGCTC | 1 cut(s) | 379 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 556 |
| EcoRII | CCWGG | 5 cut(s) | 359, 551, 605, 927, 971 |
| EcoT14I | CCWWGG | 1 cut(s) | 401 |
| EcoT22I | ATGCAT | 1 cut(s) | 846 |
| EcoT38I | GRGCYC | 1 cut(s) | 381 |
| ErhI | CCWWGG | 1 cut(s) | 401 |
| Esp3I | CGTCTC | 1 cut(s) | 699 |
| FaeI | CATG | 7 cut(s) | 136, 405, 440, 659, 668, 761, 1054 |
| FaqI | GGGAC | 2 cut(s) | 610, 805 |
| FatI | CATG | 7 cut(s) | 132, 401, 436, 655, 664, 757, 1050 |
| FbaI | TGATCA | 2 cut(s) | 69, 661 |
| FblI | GTMKAC | 1 cut(s) | 892 |
| Fnu4HI | GCNGC | 1 cut(s) | 627 |
| FokI | GGATG | 2 cut(s) | 659, 900 |
| FriOI | GRGCYC | 1 cut(s) | 381 |
| Fsp4HI | GCNGC | 1 cut(s) | 627 |
| FspBI | CTAG | 4 cut(s) | 11, 155, 581, 701 |
| GlaI | GCGC | 1 cut(s) | 396 |
| GluI | GCNGC | 1 cut(s) | 627 |
| GsaI | CCCAGC | 2 cut(s) | 456, 626 |
| GsuI | CTGGAG | 3 cut(s) | 170, 574, 911 |
| HaeIII | GGCC | 1 cut(s) | 192 |
| HapII | CCGG | 5 cut(s) | 125, 187, 198, 355, 577 |
| HgaI | GACGC | 4 cut(s) | 338, 373, 591, 903 |
| HhaI | GCGC | 1 cut(s) | 397 |
| Hin1I | GRCGYC | 1 cut(s) | 602 |
| Hin1II | CATG | 7 cut(s) | 136, 405, 440, 659, 668, 761, 1054 |
| Hin6I | GCGC | 1 cut(s) | 395 |
| HinP1I | GCGC | 1 cut(s) | 395 |
| HindIII | AAGCTT | 2 cut(s) | 54, 762 |
| HinfI | GANTC | 5 cut(s) | 30, 41, 924, 956, 1007 |
| HpaII | CCGG | 5 cut(s) | 125, 187, 198, 355, 577 |
| HphI | GGTGA | 4 cut(s) | 64, 344, 428, 671 |
| Hpy166II | GTNNAC | 4 cut(s) | 464, 688, 725, 893 |
| Hpy188I | TCNGA | 6 cut(s) | 121, 834, 955, 982, 994, 1012 |
| Hpy188III | TCNNGA | 5 cut(s) | 125, 374, 470, 494, 938 |
| Hpy8I | GTNNAC | 4 cut(s) | 464, 688, 725, 893 |
| Hpy99I | CGWCG | 2 cut(s) | 471, 720 |
| HpyAV | CCTTC | 4 cut(s) | 380, 467, 517, 1050 |
| HpyCH4III | ACNGT | 3 cut(s) | 170, 489, 709 |
| HpyCH4IV | ACGT | 2 cut(s) | 466, 715 |
| HpyCH4V | TGCA | 7 cut(s) | 52, 436, 484, 770, 844, 1050, 1054 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 53, 385, 394, 449, 573, 1028 |
| HpyF3I | CTNAG | 2 cut(s) | 143, 535 |
| HpySE526I | ACGT | 2 cut(s) | 466, 715 |
| Hsp92I | GRCGYC | 1 cut(s) | 602 |
| Hsp92II | CATG | 7 cut(s) | 136, 405, 440, 659, 668, 761, 1054 |
| HspAI | GCGC | 1 cut(s) | 395 |
| Kpn2I | TCCGGA | 1 cut(s) | 124 |
| KroI | GCCGGC | 1 cut(s) | 576 |
| KroNI | GCCGGC | 1 cut(s) | 578 |
| Ksp22I | TGATCA | 2 cut(s) | 69, 661 |
| KspI | CCGCGG | 1 cut(s) | 48 |
| Kzo9I | GATC | 4 cut(s) | 69, 121, 523, 661 |
| LguI | GCTCTTC | 1 cut(s) | 939 |
| LmnI | GCTCC | 2 cut(s) | 151, 372 |
| Lsp1109I | GCAGC | 1 cut(s) | 613 |
| LweI | GCATC | 4 cut(s) | 265, 471, 487, 563 |
| MaeI | CTAG | 4 cut(s) | 11, 155, 581, 701 |
| MaeII | ACGT | 2 cut(s) | 466, 715 |
| MaeIII | GTNAC | 1 cut(s) | 350 |
| MalI | GATC | 4 cut(s) | 71, 123, 525, 663 |
| MboI | GATC | 4 cut(s) | 69, 121, 523, 661 |
| MboII | GAAGA | 2 cut(s) | 847, 926 |
| MfeI | CAATTG | 1 cut(s) | 65 |
| MhlI | GDGCHC | 1 cut(s) | 381 |
| MluCI | AATT | 4 cut(s) | 19, 65, 228, 1000 |
| MluI | ACGCGT | 1 cut(s) | 848 |
| MlyI | GAGTC | 3 cut(s) | 35, 918, 1016 |
| MnlI | CCTC | 6 cut(s) | 686, 766, 867, 901, 974, 977 |
| Mph1103I | ATGCAT | 1 cut(s) | 846 |
| MroI | TCCGGA | 1 cut(s) | 124 |
| MroNI | GCCGGC | 1 cut(s) | 576 |
| MseI | TTAA | 2 cut(s) | 423, 861 |
| MslI | CAYNNNNRTG | 1 cut(s) | 267 |
| MspA1I | CMGCKG | 2 cut(s) | 47, 626 |
| MspI | CCGG | 5 cut(s) | 125, 187, 198, 355, 577 |
| MspR9I | CCNGG | 6 cut(s) | 188, 361, 553, 607, 929, 973 |
| MunI | CAATTG | 1 cut(s) | 65 |
| MvaI | CCWGG | 5 cut(s) | 361, 553, 607, 929, 973 |
| MvnI | CGCG | 2 cut(s) | 47, 850 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 53, 385, 394, 449, 573, 1028 |
| NaeI | GCCGGC | 1 cut(s) | 578 |
| NciI | CCSGG | 1 cut(s) | 188 |
| NcoI | CCATGG | 1 cut(s) | 401 |
| NdeII | GATC | 4 cut(s) | 69, 121, 523, 661 |
| NgoMIV | GCCGGC | 1 cut(s) | 576 |
| NlaIII | CATG | 7 cut(s) | 136, 405, 440, 659, 668, 761, 1054 |
| NlaIV | GGNNCC | 2 cut(s) | 191, 809 |
| NmeAIII | GCCGAG | 1 cut(s) | 521 |
| NmuCI | GTSAC | 1 cut(s) | 350 |
| NsiI | ATGCAT | 1 cut(s) | 846 |
| NspI | RCATGY | 2 cut(s) | 440, 1054 |
| PaeI | GCATGC | 1 cut(s) | 1054 |
| PasI | CCCWGGG | 1 cut(s) | 972 |
| PciSI | GCTCTTC | 1 cut(s) | 939 |
| PdiI | GCCGGC | 1 cut(s) | 578 |
| PfeI | GAWTC | 2 cut(s) | 30, 956 |
| PflFI | GACNNNGTC | 1 cut(s) | 601 |
| PfoI | TCCNGGA | 1 cut(s) | 605 |
| PkrI | GCNGC | 1 cut(s) | 628 |
| PleI | GAGTC | 3 cut(s) | 35, 918, 1015 |
| PpsI | GAGTC | 3 cut(s) | 35, 918, 1015 |
| PshAI | GACNNNNGTC | 1 cut(s) | 113 |
| Psp124BI | GAGCTC | 1 cut(s) | 381 |
| Psp6I | CCWGG | 5 cut(s) | 359, 551, 605, 927, 971 |
| PspFI | CCCAGC | 2 cut(s) | 452, 622 |
| PspGI | CCWGG | 5 cut(s) | 359, 551, 605, 927, 971 |
| PspN4I | GGNNCC | 2 cut(s) | 191, 809 |
| PspPI | GGNCC | 2 cut(s) | 190, 357 |
| PsyI | GACNNNGTC | 1 cut(s) | 601 |
| PvuII | CAGCTG | 1 cut(s) | 626 |
| RsaI | GTAC | 3 cut(s) | 131, 465, 689 |
| RsaNI | GTAC | 3 cut(s) | 130, 464, 688 |
| RseI | CAYNNNNRTG | 1 cut(s) | 267 |
| SacI | GAGCTC | 1 cut(s) | 381 |
| SacII | CCGCGG | 1 cut(s) | 48 |
| SapI | GCTCTTC | 1 cut(s) | 939 |
| SaqAI | TTAA | 2 cut(s) | 423, 861 |
| SatI | GCNGC | 1 cut(s) | 627 |
| Sau3AI | GATC | 4 cut(s) | 69, 121, 523, 661 |
| Sau96I | GGNCC | 2 cut(s) | 190, 357 |
| SchI | GAGTC | 3 cut(s) | 35, 918, 1016 |
| ScrFI | CCNGG | 6 cut(s) | 188, 361, 553, 607, 929, 973 |
| SduI | GDGCHC | 1 cut(s) | 381 |
| SfaNI | GCATC | 4 cut(s) | 265, 471, 487, 563 |
| Sfr303I | CCGCGG | 1 cut(s) | 48 |
| SgrBI | CCGCGG | 1 cut(s) | 48 |
| SinI | GGWCC | 1 cut(s) | 357 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 267 |
| SphI | GCATGC | 1 cut(s) | 1054 |
| Sse9I | AATT | 4 cut(s) | 19, 65, 228, 1000 |
| SsiI | CCGC | 2 cut(s) | 45, 47 |
| SspMI | CTAG | 4 cut(s) | 11, 155, 581, 701 |
| SstI | GAGCTC | 1 cut(s) | 381 |
| StyD4I | CCNGG | 6 cut(s) | 186, 359, 551, 605, 927, 971 |
| StyI | CCWWGG | 1 cut(s) | 401 |
| TaaI | ACNGT | 3 cut(s) | 170, 489, 709 |
| TaiI | ACGT | 2 cut(s) | 469, 718 |
| TaqII | GACCGA | 1 cut(s) | 234 |
| TasI | AATT | 4 cut(s) | 19, 65, 228, 1000 |
| TatI | WGTACW | 1 cut(s) | 129 |
| TfiI | GAWTC | 2 cut(s) | 30, 956 |
| Tru1I | TTAA | 2 cut(s) | 423, 861 |
| Tru9I | TTAA | 2 cut(s) | 423, 861 |
| TscAI | CASTG | 3 cut(s) | 349, 597, 712 |
| TseFI | GTSAC | 1 cut(s) | 350 |
| TseI | GCWGC | 1 cut(s) | 626 |
| Tsp45I | GTSAC | 1 cut(s) | 350 |
| TspDTI | ATGAA | 3 cut(s) | 441, 644, 774 |
| TspGWI | ACGGA | 2 cut(s) | 722, 820 |
| TspRI | CASTG | 3 cut(s) | 349, 597, 712 |
| Tth111I | GACNNNGTC | 1 cut(s) | 601 |
| VpaK11BI | GGWCC | 1 cut(s) | 357 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 556 |
| XceI | RCATGY | 2 cut(s) | 440, 1054 |
| XmiI | GTMKAC | 1 cut(s) | 892 |
| XspI | CTAG | 4 cut(s) | 11, 155, 581, 701 |
| Zsp2I | ATGCAT | 1 cut(s) | 846 |