ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 863 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 60 |
| Acc36I | ACCTGC | 1 cut(s) | 592 |
| AccB1I | GGYRCC | 1 cut(s) | 686 |
| AccI | GTMKAC | 1 cut(s) | 221 |
| AccII | CGCG | 5 cut(s) | 296, 762, 776, 788, 799 |
| AciI | CCGC | 5 cut(s) | 404, 624, 760, 788, 962 |
| AclWI | GGATC | 3 cut(s) | 745, 772, 817 |
| AcsI | RAATTY | 4 cut(s) | 110, 857, 1147, 1212 |
| AfaI | GTAC | 4 cut(s) | 238, 552, 664, 792 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 683, 787, 843 |
| AflIII | ACRYGT | 2 cut(s) | 286, 665 |
| AgsI | TTSAA | 3 cut(s) | 188, 920, 952 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 306 |
| AjnI | CCWGG | 3 cut(s) | 459, 849, 1116 |
| AleI | CACNNNNGTG | 1 cut(s) | 205 |
| AluBI | AGCT | 3 cut(s) | 175, 384, 1129 |
| AluI | AGCT | 3 cut(s) | 175, 384, 1129 |
| Alw21I | GWGCWC | 1 cut(s) | 294 |
| Alw26I | GTCTC | 6 cut(s) | 11, 131, 574, 592, 702, 893 |
| AlwI | GGATC | 3 cut(s) | 745, 772, 817 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 260 |
| ApeKI | GCWGC | 1 cut(s) | 1129 |
| ApoI | RAATTY | 4 cut(s) | 110, 857, 1147, 1212 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 604, 636 |
| AseI | ATTAAT | 1 cut(s) | 270 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 438 |
| AspLEI | GCGC | 2 cut(s) | 725, 1134 |
| AspS9I | GGNCC | 1 cut(s) | 846 |
| AsuC2I | CCSGG | 1 cut(s) | 735 |
| AsuHPI | GGTGA | 7 cut(s) | 107, 189, 203, 241, 431, 995, 1211 |
| AvaII | GGWCC | 1 cut(s) | 846 |
| BanI | GGYRCC | 1 cut(s) | 686 |
| BauI | CACGAG | 1 cut(s) | 243 |
| BbsI | GAAGAC | 1 cut(s) | 305 |
| Bbv12I | GWGCWC | 1 cut(s) | 294 |
| BbvI | GCAGC | 1 cut(s) | 1116 |
| BccI | CCATC | 3 cut(s) | 480, 889, 980 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 590, 624, 635, 669 |
| BciT130I | CCWGG | 3 cut(s) | 461, 851, 1118 |
| BclI | TGATCA | 1 cut(s) | 628 |
| BcnI | CCSGG | 1 cut(s) | 735 |
| BcoDI | GTCTC | 6 cut(s) | 11, 131, 574, 592, 702, 893 |
| BfaI | CTAG | 4 cut(s) | 276, 371, 560, 743 |
| BfuAI | ACCTGC | 1 cut(s) | 592 |
| BisI | GCNGC | 2 cut(s) | 404, 1130 |
| BlpI | GCTNAGC | 1 cut(s) | 719 |
| BlsI | GCNGC | 2 cut(s) | 405, 1131 |
| BmcAI | AGTACT | 1 cut(s) | 238 |
| Bme1390I | CCNGG | 4 cut(s) | 461, 735, 851, 1118 |
| Bme18I | GGWCC | 1 cut(s) | 846 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 306 |
| BmgT120I | GGNCC | 1 cut(s) | 846 |
| BmiI | GGNNCC | 2 cut(s) | 688, 848 |
| BmrFI | CCNGG | 4 cut(s) | 461, 735, 851, 1118 |
| BmsI | GCATC | 4 cut(s) | 201, 720, 849, 1048 |
| BpiI | GAAGAC | 1 cut(s) | 305 |
| BpmI | CTGGAG | 1 cut(s) | 801 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 719 |
| BpuMI | CCSGG | 1 cut(s) | 735 |
| BsaAI | YACGTR | 1 cut(s) | 666 |
| BsaBI | GATNNNNATC | 1 cut(s) | 782 |
| BsaJI | CCNNGG | 2 cut(s) | 215, 849 |
| BsaWI | WCCGGW | 1 cut(s) | 521 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 344, 374 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 683, 787, 843 |
| Bse118I | RCCGGY | 1 cut(s) | 751 |
| Bse1I | ACTGG | 1 cut(s) | 1105 |
| Bse8I | GATNNNNATC | 1 cut(s) | 782 |
| BseBI | CCWGG | 3 cut(s) | 461, 851, 1118 |
| BseDI | CCNNGG | 2 cut(s) | 215, 849 |
| BseGI | GGATG | 5 cut(s) | 19, 409, 472, 571, 1225 |
| BseJI | GATNNNNATC | 1 cut(s) | 782 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 683, 787, 843 |
| BseMII | CTCAG | 2 cut(s) | 710, 903 |
| BseNI | ACTGG | 1 cut(s) | 1105 |
| BseXI | GCAGC | 1 cut(s) | 1116 |
| BseYI | CCCAGC | 2 cut(s) | 836, 1125 |
| Bsh1236I | CGCG | 5 cut(s) | 296, 762, 776, 788, 799 |
| Bsh1285I | CGRYCG | 1 cut(s) | 797 |
| BshNI | GGYRCC | 1 cut(s) | 686 |
| BsiEI | CGRYCG | 1 cut(s) | 797 |
| BsiHKAI | GWGCWC | 1 cut(s) | 294 |
| BsiSI | CCGG | 3 cut(s) | 522, 734, 752 |
| BsiWI | CGTACG | 1 cut(s) | 662 |
| BslFI | GGGAC | 3 cut(s) | 606, 832, 881 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 683, 787, 843 |
| BsmAI | GTCTC | 6 cut(s) | 11, 131, 574, 592, 702, 893 |
| BsmBI | CGTCTC | 2 cut(s) | 11, 131 |
| BsmFI | GGGAC | 3 cut(s) | 606, 832, 881 |
| Bsp1286I | GDGCHC | 1 cut(s) | 294 |
| Bsp143I | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 719 |
| Bsp68I | TCGCGA | 2 cut(s) | 296, 799 |
| BspACI | CCGC | 5 cut(s) | 404, 624, 760, 788, 962 |
| BspCNI | CTCAG | 2 cut(s) | 711, 904 |
| BspFNI | CGCG | 5 cut(s) | 296, 762, 776, 788, 799 |
| BspLI | GGNNCC | 2 cut(s) | 688, 848 |
| BspMI | ACCTGC | 1 cut(s) | 592 |
| BspPI | GGATC | 3 cut(s) | 745, 772, 817 |
| BspT107I | GGYRCC | 1 cut(s) | 686 |
| BsrFI | RCCGGY | 1 cut(s) | 751 |
| BsrI | ACTGG | 1 cut(s) | 1105 |
| BssAI | RCCGGY | 1 cut(s) | 751 |
| BssECI | CCNNGG | 2 cut(s) | 215, 849 |
| BssMI | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| BssSI | CACGAG | 1 cut(s) | 243 |
| Bst2BI | CACGAG | 1 cut(s) | 243 |
| Bst2UI | CCWGG | 3 cut(s) | 461, 851, 1118 |
| Bst4CI | ACNGT | 5 cut(s) | 207, 219, 304, 482, 550 |
| BstBAI | YACGTR | 1 cut(s) | 666 |
| BstC8I | GCNNGC | 5 cut(s) | 294, 330, 599, 753, 1187 |
| BstDEI | CTNAG | 3 cut(s) | 693, 719, 912 |
| BstDSI | CCRYGG | 1 cut(s) | 215 |
| BstF5I | GGATG | 5 cut(s) | 19, 409, 472, 571, 1225 |
| BstFNI | CGCG | 5 cut(s) | 296, 762, 776, 788, 799 |
| BstHHI | GCGC | 2 cut(s) | 725, 1134 |
| BstKTI | GATC | 7 cut(s) | 631, 740, 772, 780, 797, 809, 825 |
| BstMAI | GTCTC | 6 cut(s) | 11, 131, 574, 592, 702, 893 |
| BstMBI | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| BstMCI | CGRYCG | 1 cut(s) | 797 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 632, 739, 748 |
| BstNI | CCWGG | 3 cut(s) | 461, 851, 1118 |
| BstNSI | RCATGY | 2 cut(s) | 290, 601 |
| BstSCI | CCNGG | 4 cut(s) | 459, 733, 849, 1116 |
| BstUI | CGCG | 5 cut(s) | 296, 762, 776, 788, 799 |
| BstV1I | GCAGC | 1 cut(s) | 1116 |
| BstV2I | GAAGAC | 1 cut(s) | 305 |
| BtgI | CCRYGG | 1 cut(s) | 215 |
| BtgZI | GCGATG | 1 cut(s) | 627 |
| BtsCI | GGATG | 5 cut(s) | 19, 409, 472, 571, 1225 |
| BtsIMutI | CAGTG | 1 cut(s) | 1098 |
| BtuMI | TCGCGA | 2 cut(s) | 296, 799 |
| BveI | ACCTGC | 1 cut(s) | 592 |
| Cac8I | GCNNGC | 5 cut(s) | 294, 330, 599, 753, 1187 |
| CaiI | CAGNNNCTG | 1 cut(s) | 260 |
| CfoI | GCGC | 2 cut(s) | 725, 1134 |
| Cfr10I | RCCGGY | 1 cut(s) | 751 |
| Cfr13I | GGNCC | 1 cut(s) | 846 |
| Csp6I | GTAC | 4 cut(s) | 237, 551, 663, 791 |
| CviAII | CATG | 4 cut(s) | 287, 517, 598, 1143 |
| CviJI | RGCY | 9 cut(s) | 40, 175, 384, 403, 635, 718, 1129, 1185, 1189 |
| CviKI_1 | RGCY | 9 cut(s) | 40, 175, 384, 403, 635, 718, 1129, 1185, 1189 |
| CviQI | GTAC | 4 cut(s) | 237, 551, 663, 791 |
| DdeI | CTNAG | 3 cut(s) | 693, 719, 912 |
| DpnI | GATC | 7 cut(s) | 630, 739, 771, 779, 796, 808, 824 |
| DpnII | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| DraI | TTTAAA | 1 cut(s) | 874 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 60 |
| DriI | GACNNNNNGTC | 1 cut(s) | 306 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 60 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 306 |
| Eco47I | GGWCC | 1 cut(s) | 846 |
| EcoO109I | RGGNCCY | 1 cut(s) | 846 |
| EcoRI | GAATTC | 1 cut(s) | 110 |
| EcoRII | CCWGG | 3 cut(s) | 459, 849, 1116 |
| Esp3I | CGTCTC | 2 cut(s) | 11, 131 |
| FaeI | CATG | 4 cut(s) | 290, 520, 601, 1146 |
| FaqI | GGGAC | 3 cut(s) | 606, 832, 881 |
| FatI | CATG | 4 cut(s) | 286, 516, 597, 1142 |
| FauI | CCCGC | 2 cut(s) | 631, 767 |
| FbaI | TGATCA | 1 cut(s) | 628 |
| FblI | GTMKAC | 1 cut(s) | 221 |
| Fnu4HI | GCNGC | 2 cut(s) | 404, 1130 |
| FokI | GGATG | 5 cut(s) | 26, 396, 459, 578, 1232 |
| Fsp4HI | GCNGC | 2 cut(s) | 404, 1130 |
| FspBI | CTAG | 4 cut(s) | 276, 371, 560, 743 |
| GlaI | GCGC | 2 cut(s) | 724, 1133 |
| GluI | GCNGC | 2 cut(s) | 404, 1130 |
| GsaI | CCCAGC | 2 cut(s) | 840, 1129 |
| GsuI | CTGGAG | 1 cut(s) | 801 |
| HapII | CCGG | 3 cut(s) | 522, 734, 752 |
| HhaI | GCGC | 2 cut(s) | 725, 1134 |
| Hin1II | CATG | 4 cut(s) | 290, 520, 601, 1146 |
| Hin6I | GCGC | 2 cut(s) | 723, 1132 |
| HinP1I | GCGC | 2 cut(s) | 723, 1132 |
| HindIII | AAGCTT | 1 cut(s) | 173 |
| HinfI | GANTC | 9 cut(s) | 257, 434, 494, 527, 638, 671, 783, 1121, 1161 |
| HpaII | CCGG | 3 cut(s) | 522, 734, 752 |
| HphI | GGTGA | 7 cut(s) | 107, 189, 203, 241, 431, 995, 1211 |
| Hpy166II | GTNNAC | 2 cut(s) | 222, 659 |
| Hpy188I | TCNGA | 4 cut(s) | 453, 782, 1015, 1228 |
| Hpy188III | TCNNGA | 6 cut(s) | 295, 571, 767, 798, 892, 1076 |
| Hpy8I | GTNNAC | 2 cut(s) | 222, 659 |
| HpyAV | CCTTC | 2 cut(s) | 512, 606 |
| HpyCH4III | ACNGT | 5 cut(s) | 207, 219, 304, 482, 550 |
| HpyCH4IV | ACGT | 5 cut(s) | 20, 124, 619, 661, 665 |
| HpyCH4V | TGCA | 3 cut(s) | 30, 516, 601 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 632, 739, 748 |
| HpyF3I | CTNAG | 3 cut(s) | 693, 719, 912 |
| HpySE526I | ACGT | 5 cut(s) | 20, 124, 619, 661, 665 |
| Hsp92II | CATG | 4 cut(s) | 290, 520, 601, 1146 |
| HspAI | GCGC | 2 cut(s) | 723, 1132 |
| KroI | GCCGGC | 1 cut(s) | 751 |
| KroNI | GCCGGC | 1 cut(s) | 753 |
| Ksp22I | TGATCA | 1 cut(s) | 628 |
| Kzo9I | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| Lsp1109I | GCAGC | 1 cut(s) | 1116 |
| LweI | GCATC | 4 cut(s) | 201, 720, 849, 1048 |
| MaeI | CTAG | 4 cut(s) | 276, 371, 560, 743 |
| MaeII | ACGT | 5 cut(s) | 20, 124, 619, 661, 665 |
| MaeIII | GTNAC | 4 cut(s) | 209, 334, 811, 1199 |
| MalI | GATC | 7 cut(s) | 630, 739, 771, 779, 796, 808, 824 |
| MboI | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| MboII | GAAGA | 2 cut(s) | 164, 310 |
| MhlI | GDGCHC | 1 cut(s) | 294 |
| MlyI | GAGTC | 2 cut(s) | 632, 1155 |
| MmeI | TCCRAC | 1 cut(s) | 993 |
| MroNI | GCCGGC | 1 cut(s) | 751 |
| MroXI | GAANNNNTTC | 1 cut(s) | 438 |
| MseI | TTAA | 6 cut(s) | 42, 177, 266, 270, 375, 873 |
| MslI | CAYNNNNRTG | 2 cut(s) | 205, 1056 |
| MspA1I | CMGCKG | 2 cut(s) | 626, 1129 |
| MspI | CCGG | 3 cut(s) | 522, 734, 752 |
| MspR9I | CCNGG | 4 cut(s) | 461, 735, 851, 1118 |
| MvaI | CCWGG | 3 cut(s) | 461, 851, 1118 |
| MvnI | CGCG | 5 cut(s) | 296, 762, 776, 788, 799 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 632, 739, 748 |
| NaeI | GCCGGC | 1 cut(s) | 753 |
| NciI | CCSGG | 1 cut(s) | 735 |
| NdeII | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| NgoMIV | GCCGGC | 1 cut(s) | 751 |
| NlaIII | CATG | 4 cut(s) | 290, 520, 601, 1146 |
| NlaIV | GGNNCC | 2 cut(s) | 688, 848 |
| NmuCI | GTSAC | 2 cut(s) | 209, 1199 |
| NruI | TCGCGA | 2 cut(s) | 296, 799 |
| NspI | RCATGY | 2 cut(s) | 290, 601 |
| OliI | CACNNNNGTG | 1 cut(s) | 205 |
| PacI | TTAATTAA | 1 cut(s) | 270 |
| PaeI | GCATGC | 1 cut(s) | 601 |
| PciI | ACATGT | 1 cut(s) | 286 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 616 |
| PdiI | GCCGGC | 1 cut(s) | 753 |
| PdmI | GAANNNNTTC | 1 cut(s) | 438 |
| PfeI | GAWTC | 7 cut(s) | 257, 434, 494, 527, 671, 783, 1121 |
| Pfl23II | CGTACG | 1 cut(s) | 662 |
| PkrI | GCNGC | 2 cut(s) | 405, 1131 |
| Ple19I | CGATCG | 1 cut(s) | 797 |
| PleI | GAGTC | 2 cut(s) | 632, 1155 |
| PpsI | GAGTC | 2 cut(s) | 632, 1155 |
| Ppu21I | YACGTR | 1 cut(s) | 666 |
| PpuMI | RGGWCCY | 1 cut(s) | 846 |
| PscI | ACATGT | 1 cut(s) | 286 |
| PshBI | ATTAAT | 1 cut(s) | 270 |
| PsiI | TTATAA | 1 cut(s) | 863 |
| Psp5II | RGGWCCY | 1 cut(s) | 846 |
| Psp6I | CCWGG | 3 cut(s) | 459, 849, 1116 |
| PspFI | CCCAGC | 2 cut(s) | 836, 1125 |
| PspGI | CCWGG | 3 cut(s) | 459, 849, 1116 |
| PspLI | CGTACG | 1 cut(s) | 662 |
| PspN4I | GGNNCC | 2 cut(s) | 688, 848 |
| PspPI | GGNCC | 1 cut(s) | 846 |
| PspPPI | RGGWCCY | 1 cut(s) | 846 |
| PstNI | CAGNNNCTG | 1 cut(s) | 260 |
| PvuI | CGATCG | 1 cut(s) | 797 |
| PvuII | CAGCTG | 1 cut(s) | 1129 |
| RruI | TCGCGA | 2 cut(s) | 296, 799 |
| RsaI | GTAC | 4 cut(s) | 238, 552, 664, 792 |
| RsaNI | GTAC | 4 cut(s) | 237, 551, 663, 791 |
| RseI | CAYNNNNRTG | 2 cut(s) | 205, 1056 |
| SaqAI | TTAA | 6 cut(s) | 42, 177, 266, 270, 375, 873 |
| SatI | GCNGC | 2 cut(s) | 404, 1130 |
| Sau3AI | GATC | 7 cut(s) | 628, 737, 769, 777, 794, 806, 822 |
| Sau96I | GGNCC | 1 cut(s) | 846 |
| ScaI | AGTACT | 1 cut(s) | 238 |
| SchI | GAGTC | 2 cut(s) | 632, 1155 |
| ScrFI | CCNGG | 4 cut(s) | 461, 735, 851, 1118 |
| SduI | GDGCHC | 1 cut(s) | 294 |
| SfaNI | GCATC | 4 cut(s) | 201, 720, 849, 1048 |
| SinI | GGWCC | 1 cut(s) | 846 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 205, 1056 |
| SphI | GCATGC | 1 cut(s) | 601 |
| SsiI | CCGC | 5 cut(s) | 404, 624, 760, 788, 962 |
| SspMI | CTAG | 4 cut(s) | 276, 371, 560, 743 |
| StyD4I | CCNGG | 4 cut(s) | 459, 733, 849, 1116 |
| TaaI | ACNGT | 5 cut(s) | 207, 219, 304, 482, 550 |
| TaiI | ACGT | 5 cut(s) | 23, 127, 622, 664, 668 |
| TaqI | TCGA | 6 cut(s) | 64, 399, 572, 610, 707, 939 |
| TatI | WGTACW | 1 cut(s) | 236 |
| TauI | GCSGC | 1 cut(s) | 406 |
| TfiI | GAWTC | 7 cut(s) | 257, 434, 494, 527, 671, 783, 1121 |
| Tru1I | TTAA | 6 cut(s) | 42, 177, 266, 270, 375, 873 |
| Tru9I | TTAA | 6 cut(s) | 42, 177, 266, 270, 375, 873 |
| TscAI | CASTG | 1 cut(s) | 1105 |
| TseFI | GTSAC | 2 cut(s) | 209, 1199 |
| TseI | GCWGC | 1 cut(s) | 1129 |
| Tsp45I | GTSAC | 2 cut(s) | 209, 1199 |
| TspDTI | ATGAA | 3 cut(s) | 102, 135, 1058 |
| TspRI | CASTG | 1 cut(s) | 1105 |
| VpaK11BI | GGWCC | 1 cut(s) | 846 |
| VspI | ATTAAT | 1 cut(s) | 270 |
| XapI | RAATTY | 4 cut(s) | 110, 857, 1147, 1212 |
| XceI | RCATGY | 2 cut(s) | 290, 601 |
| XmiI | GTMKAC | 1 cut(s) | 221 |
| XmnI | GAANNNNTTC | 1 cut(s) | 438 |
| XspI | CTAG | 4 cut(s) | 276, 371, 560, 743 |
| ZrmI | AGTACT | 1 cut(s) | 238 |