ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 145 |
| AccI | GTMKAC | 1 cut(s) | 65 |
| AciI | CCGC | 2 cut(s) | 145, 154 |
| AclWI | GGATC | 3 cut(s) | 390, 403, 722 |
| AcoI | YGGCCR | 1 cut(s) | 415 |
| AcsI | RAATTY | 2 cut(s) | 446, 1344 |
| AfaI | GTAC | 1 cut(s) | 268 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 693 |
| AflIII | ACRYGT | 2 cut(s) | 541, 550 |
| AgsI | TTSAA | 8 cut(s) | 61, 127, 195, 263, 853, 907, 1084, 1199 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 737, 769 |
| AluBI | AGCT | 5 cut(s) | 469, 748, 1040, 1288, 1435 |
| AluI | AGCT | 5 cut(s) | 469, 748, 1040, 1288, 1435 |
| Alw21I | GWGCWC | 2 cut(s) | 471, 1431 |
| Alw26I | GTCTC | 1 cut(s) | 1062 |
| Alw44I | GTGCAC | 1 cut(s) | 1427 |
| AlwI | GGATC | 3 cut(s) | 390, 403, 722 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1043 |
| Ama87I | CYCGRG | 2 cut(s) | 606, 928 |
| AoxI | GGCC | 4 cut(s) | 415, 931, 954, 1098 |
| ApaLI | GTGCAC | 1 cut(s) | 1427 |
| ApeKI | GCWGC | 5 cut(s) | 77, 156, 178, 895, 1037 |
| ApoI | RAATTY | 2 cut(s) | 446, 1344 |
| AspA2I | CCTAGG | 1 cut(s) | 32 |
| AspLEI | GCGC | 2 cut(s) | 46, 507 |
| AspS9I | GGNCC | 4 cut(s) | 26, 151, 931, 954 |
| AsuC2I | CCSGG | 2 cut(s) | 929, 930 |
| AsuHPI | GGTGA | 3 cut(s) | 199, 641, 725 |
| AvaI | CYCGRG | 2 cut(s) | 606, 928 |
| AvaII | GGWCC | 2 cut(s) | 26, 151 |
| AvrII | CCTAGG | 1 cut(s) | 32 |
| BaeGI | GKGCMC | 1 cut(s) | 1431 |
| BamHI | GGATCC | 1 cut(s) | 395 |
| BanII | GRGCYC | 1 cut(s) | 471 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 732, 764 |
| Bbv12I | GWGCWC | 2 cut(s) | 471, 1431 |
| BbvI | GCAGC | 5 cut(s) | 64, 143, 190, 882, 1049 |
| BccI | CCATC | 4 cut(s) | 226, 620, 924, 931 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 587, 621 |
| BclI | TGATCA | 2 cut(s) | 1216, 1350 |
| BcnI | CCSGG | 2 cut(s) | 929, 930 |
| BcoDI | GTCTC | 1 cut(s) | 1062 |
| BfaI | CTAG | 2 cut(s) | 33, 372 |
| BfmI | CTRYAG | 1 cut(s) | 732 |
| BglII | AGATCT | 1 cut(s) | 602 |
| BisI | GCNGC | 5 cut(s) | 78, 157, 179, 896, 1038 |
| BlnI | CCTAGG | 1 cut(s) | 32 |
| BlsI | GCNGC | 5 cut(s) | 79, 158, 180, 897, 1039 |
| Bme1390I | CCNGG | 2 cut(s) | 929, 930 |
| Bme18I | GGWCC | 2 cut(s) | 26, 151 |
| BmeT110I | CYCGRG | 2 cut(s) | 606, 928 |
| BmgT120I | GGNCC | 4 cut(s) | 26, 151, 931, 954 |
| BmiI | GGNNCC | 3 cut(s) | 397, 955, 987 |
| BmrFI | CCNGG | 2 cut(s) | 929, 930 |
| BmsI | GCATC | 4 cut(s) | 790, 921, 1006, 1362 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1085, 1117, 1274, 1306 |
| BpmI | CTGGAG | 1 cut(s) | 613 |
| BpuMI | CCSGG | 2 cut(s) | 929, 930 |
| BsaAI | YACGTR | 1 cut(s) | 278 |
| BsaBI | GATNNNNATC | 4 cut(s) | 228, 234, 630, 1215 |
| BsaJI | CCNNGG | 3 cut(s) | 32, 521, 928 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 199, 229 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 693 |
| Bse118I | RCCGGY | 1 cut(s) | 413 |
| Bse1I | ACTGG | 2 cut(s) | 163, 596 |
| Bse3DI | GCAATG | 3 cut(s) | 359, 437, 1392 |
| Bse8I | GATNNNNATC | 4 cut(s) | 228, 234, 630, 1215 |
| BseDI | CCNNGG | 3 cut(s) | 32, 521, 928 |
| BseGI | GGATG | 4 cut(s) | 631, 662, 912, 916 |
| BseJI | GATNNNNATC | 4 cut(s) | 228, 234, 630, 1215 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 693 |
| BseMI | GCAATG | 3 cut(s) | 359, 437, 1392 |
| BseMII | CTCAG | 4 cut(s) | 456, 774, 890, 1274 |
| BseNI | ACTGG | 2 cut(s) | 163, 596 |
| BseRI | GAGGAG | 1 cut(s) | 320 |
| BseSI | GKGCMC | 1 cut(s) | 1431 |
| BseXI | GCAGC | 5 cut(s) | 64, 143, 190, 882, 1049 |
| BsgI | GTGCAG | 1 cut(s) | 1353 |
| BshFI | GGCC | 4 cut(s) | 417, 933, 956, 1100 |
| BsiHKAI | GWGCWC | 2 cut(s) | 471, 1431 |
| BsiHKCI | CYCGRG | 2 cut(s) | 606, 928 |
| BsiSI | CCGG | 2 cut(s) | 414, 929 |
| BslFI | GGGAC | 3 cut(s) | 104, 911, 1242 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 693 |
| BsmAI | GTCTC | 1 cut(s) | 1062 |
| BsmBI | CGTCTC | 1 cut(s) | 1062 |
| BsmFI | GGGAC | 3 cut(s) | 104, 911, 1242 |
| BsnI | GGCC | 4 cut(s) | 417, 933, 956, 1100 |
| BsoBI | CYCGRG | 2 cut(s) | 606, 928 |
| Bsp1286I | GDGCHC | 2 cut(s) | 471, 1431 |
| Bsp143I | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| BspACI | CCGC | 2 cut(s) | 145, 154 |
| BspANI | GGCC | 4 cut(s) | 417, 933, 956, 1100 |
| BspCNI | CTCAG | 4 cut(s) | 457, 775, 889, 1273 |
| BspHI | TCATGA | 1 cut(s) | 1213 |
| BspLI | GGNNCC | 3 cut(s) | 397, 955, 987 |
| BspMAI | CTGCAG | 1 cut(s) | 736 |
| BspPI | GGATC | 3 cut(s) | 390, 403, 722 |
| BsrBI | CCGCTC | 1 cut(s) | 145 |
| BsrDI | GCAATG | 3 cut(s) | 359, 437, 1392 |
| BsrFI | RCCGGY | 1 cut(s) | 413 |
| BsrI | ACTGG | 2 cut(s) | 163, 596 |
| BssAI | RCCGGY | 1 cut(s) | 413 |
| BssECI | CCNNGG | 3 cut(s) | 32, 521, 928 |
| BssMI | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| BssT1I | CCWWGG | 2 cut(s) | 32, 521 |
| Bst4CI | ACNGT | 3 cut(s) | 57, 656, 1045 |
| Bst6I | CTCTTC | 1 cut(s) | 1121 |
| BstBAI | YACGTR | 1 cut(s) | 278 |
| BstDEI | CTNAG | 4 cut(s) | 465, 783, 876, 1260 |
| BstF5I | GGATG | 4 cut(s) | 631, 662, 912, 916 |
| BstHHI | GCGC | 2 cut(s) | 46, 507 |
| BstKTI | GATC | 9 cut(s) | 232, 398, 499, 605, 634, 730, 1213, 1219, 1353 |
| BstMAI | GTCTC | 1 cut(s) | 1062 |
| BstMBI | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 50, 1046 |
| BstNSI | RCATGY | 1 cut(s) | 554 |
| BstSCI | CCNGG | 2 cut(s) | 927, 928 |
| BstSFI | CTRYAG | 1 cut(s) | 732 |
| BstSLI | GKGCMC | 1 cut(s) | 1431 |
| BstSNI | TACGTA | 1 cut(s) | 278 |
| BstV1I | GCAGC | 5 cut(s) | 64, 143, 190, 882, 1049 |
| BstX2I | RGATCY | 2 cut(s) | 395, 602 |
| BstYI | RGATCY | 2 cut(s) | 395, 602 |
| BsuRI | GGCC | 4 cut(s) | 417, 933, 956, 1100 |
| BtsCI | GGATG | 4 cut(s) | 631, 662, 912, 916 |
| BtsI | GCAGTG | 1 cut(s) | 335 |
| BtsIMutI | CAGTG | 2 cut(s) | 53, 335 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1043 |
| CciI | TCATGA | 1 cut(s) | 1213 |
| CfoI | GCGC | 2 cut(s) | 46, 507 |
| Cfr10I | RCCGGY | 1 cut(s) | 413 |
| Cfr13I | GGNCC | 4 cut(s) | 26, 151, 931, 954 |
| Cfr9I | CCCGGG | 1 cut(s) | 928 |
| CseI | GACGC | 1 cut(s) | 83 |
| Csp6I | GTAC | 1 cut(s) | 267 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 4, 39 |
| CviQI | GTAC | 1 cut(s) | 267 |
| DdeI | CTNAG | 4 cut(s) | 465, 783, 876, 1260 |
| DpnI | GATC | 9 cut(s) | 231, 397, 498, 604, 633, 729, 1212, 1218, 1352 |
| DpnII | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| DraI | TTTAAA | 1 cut(s) | 969 |
| EaeI | YGGCCR | 1 cut(s) | 415 |
| Eam1104I | CTCTTC | 1 cut(s) | 1121 |
| EarI | CTCTTC | 1 cut(s) | 1121 |
| Ecl136II | GAGCTC | 1 cut(s) | 469 |
| Eco105I | TACGTA | 1 cut(s) | 278 |
| Eco130I | CCWWGG | 2 cut(s) | 32, 521 |
| Eco147I | AGGCCT | 1 cut(s) | 1100 |
| Eco24I | GRGCYC | 1 cut(s) | 471 |
| Eco47I | GGWCC | 2 cut(s) | 26, 151 |
| Eco53kI | GAGCTC | 1 cut(s) | 469 |
| Eco88I | CYCGRG | 2 cut(s) | 606, 928 |
| EcoICRI | GAGCTC | 1 cut(s) | 469 |
| EcoT14I | CCWWGG | 2 cut(s) | 32, 521 |
| EcoT22I | ATGCAT | 2 cut(s) | 914, 999 |
| EcoT38I | GRGCYC | 1 cut(s) | 471 |
| ErhI | CCWWGG | 2 cut(s) | 32, 521 |
| Esp3I | CGTCTC | 1 cut(s) | 1062 |
| FaqI | GGGAC | 3 cut(s) | 104, 911, 1242 |
| FbaI | TGATCA | 2 cut(s) | 1216, 1350 |
| FblI | GTMKAC | 1 cut(s) | 65 |
| Fnu4HI | GCNGC | 5 cut(s) | 78, 157, 179, 896, 1038 |
| FokI | GGATG | 4 cut(s) | 638, 649, 899, 903 |
| FriOI | GRGCYC | 1 cut(s) | 471 |
| Fsp4HI | GCNGC | 5 cut(s) | 78, 157, 179, 896, 1038 |
| FspBI | CTAG | 2 cut(s) | 33, 372 |
| GlaI | GCGC | 2 cut(s) | 45, 506 |
| GluI | GCNGC | 5 cut(s) | 78, 157, 179, 896, 1038 |
| GsuI | CTGGAG | 1 cut(s) | 613 |
| HaeIII | GGCC | 4 cut(s) | 417, 933, 956, 1100 |
| HapII | CCGG | 2 cut(s) | 414, 929 |
| HgaI | GACGC | 1 cut(s) | 83 |
| HhaI | GCGC | 2 cut(s) | 46, 507 |
| Hin6I | GCGC | 2 cut(s) | 44, 505 |
| HinP1I | GCGC | 2 cut(s) | 44, 505 |
| HindIII | AAGCTT | 1 cut(s) | 746 |
| HinfI | GANTC | 6 cut(s) | 99, 224, 562, 924, 947, 1402 |
| HpaII | CCGG | 2 cut(s) | 414, 929 |
| HphI | GGTGA | 3 cut(s) | 199, 641, 725 |
| Hpy166II | GTNNAC | 7 cut(s) | 26, 66, 269, 720, 946, 980, 1429 |
| Hpy188III | TCNNGA | 4 cut(s) | 346, 606, 608, 1214 |
| Hpy8I | GTNNAC | 7 cut(s) | 26, 66, 269, 720, 946, 980, 1429 |
| HpyAV | CCTTC | 4 cut(s) | 201, 1090, 1105, 1306 |
| HpyCH4III | ACNGT | 3 cut(s) | 57, 656, 1045 |
| HpyCH4IV | ACGT | 3 cut(s) | 265, 277, 541 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 50, 1046 |
| HpyF3I | CTNAG | 4 cut(s) | 465, 783, 876, 1260 |
| HpySE526I | ACGT | 3 cut(s) | 265, 277, 541 |
| HspAI | GCGC | 2 cut(s) | 44, 505 |
| Ksp22I | TGATCA | 2 cut(s) | 1216, 1350 |
| Kzo9I | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| LmnI | GCTCC | 3 cut(s) | 142, 991, 1034 |
| Lsp1109I | GCAGC | 5 cut(s) | 64, 143, 190, 882, 1049 |
| LweI | GCATC | 4 cut(s) | 790, 921, 1006, 1362 |
| MaeI | CTAG | 2 cut(s) | 33, 372 |
| MaeII | ACGT | 3 cut(s) | 265, 277, 541 |
| MaeIII | GTNAC | 4 cut(s) | 838, 1145, 1360, 1394 |
| MalI | GATC | 9 cut(s) | 231, 397, 498, 604, 633, 729, 1212, 1218, 1352 |
| MbiI | CCGCTC | 1 cut(s) | 145 |
| MboI | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| MboII | GAAGA | 2 cut(s) | 545, 1138 |
| MfeI | CAATTG | 2 cut(s) | 566, 1306 |
| MflI | RGATCY | 2 cut(s) | 395, 602 |
| MhlI | GDGCHC | 2 cut(s) | 471, 1431 |
| MlyI | GAGTC | 3 cut(s) | 571, 933, 941 |
| MmeI | TCCRAC | 5 cut(s) | 129, 535, 735, 1225, 1281 |
| Mph1103I | ATGCAT | 2 cut(s) | 914, 999 |
| MseI | TTAA | 6 cut(s) | 86, 368, 620, 968, 1116, 1325 |
| MslI | CAYNNNNRTG | 2 cut(s) | 321, 845 |
| MspA1I | CMGCKG | 1 cut(s) | 156 |
| MspI | CCGG | 2 cut(s) | 414, 929 |
| MspR9I | CCNGG | 2 cut(s) | 929, 930 |
| MunI | CAATTG | 2 cut(s) | 566, 1306 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 50, 1046 |
| NciI | CCSGG | 2 cut(s) | 929, 930 |
| NdeII | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| NlaIV | GGNNCC | 3 cut(s) | 397, 955, 987 |
| NmuCI | GTSAC | 2 cut(s) | 838, 1145 |
| NsiI | ATGCAT | 2 cut(s) | 914, 999 |
| NspI | RCATGY | 1 cut(s) | 554 |
| PaeR7I | CTCGAG | 1 cut(s) | 606 |
| PagI | TCATGA | 1 cut(s) | 1213 |
| PceI | AGGCCT | 1 cut(s) | 1100 |
| PciI | ACATGT | 1 cut(s) | 550 |
| PfeI | GAWTC | 3 cut(s) | 99, 224, 1402 |
| PflFI | GACNNNGTC | 1 cut(s) | 561 |
| PkrI | GCNGC | 5 cut(s) | 79, 158, 180, 897, 1039 |
| PleI | GAGTC | 3 cut(s) | 570, 932, 941 |
| PpsI | GAGTC | 3 cut(s) | 570, 932, 941 |
| Ppu21I | YACGTR | 1 cut(s) | 278 |
| PscI | ACATGT | 1 cut(s) | 550 |
| Psp124BI | GAGCTC | 1 cut(s) | 471 |
| PspN4I | GGNNCC | 3 cut(s) | 397, 955, 987 |
| PspPI | GGNCC | 4 cut(s) | 26, 151, 931, 954 |
| PstI | CTGCAG | 1 cut(s) | 736 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1043 |
| PsuI | RGATCY | 2 cut(s) | 395, 602 |
| PsyI | GACNNNGTC | 1 cut(s) | 561 |
| RsaI | GTAC | 1 cut(s) | 268 |
| RsaNI | GTAC | 1 cut(s) | 267 |
| RseI | CAYNNNNRTG | 2 cut(s) | 321, 845 |
| SacI | GAGCTC | 1 cut(s) | 471 |
| SaqAI | TTAA | 6 cut(s) | 86, 368, 620, 968, 1116, 1325 |
| SatI | GCNGC | 5 cut(s) | 78, 157, 179, 896, 1038 |
| Sau3AI | GATC | 9 cut(s) | 229, 395, 496, 602, 631, 727, 1210, 1216, 1350 |
| Sau96I | GGNCC | 4 cut(s) | 26, 151, 931, 954 |
| SchI | GAGTC | 3 cut(s) | 571, 933, 941 |
| ScrFI | CCNGG | 2 cut(s) | 929, 930 |
| SduI | GDGCHC | 2 cut(s) | 471, 1431 |
| SfaNI | GCATC | 4 cut(s) | 790, 921, 1006, 1362 |
| SfcI | CTRYAG | 1 cut(s) | 732 |
| Sfr274I | CTCGAG | 1 cut(s) | 606 |
| SinI | GGWCC | 2 cut(s) | 26, 151 |
| SlaI | CTCGAG | 1 cut(s) | 606 |
| SmaI | CCCGGG | 1 cut(s) | 930 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 321, 845 |
| SmlI | CTYRAG | 1 cut(s) | 606 |
| SmoI | CTYRAG | 1 cut(s) | 606 |
| SnaBI | TACGTA | 1 cut(s) | 278 |
| SseBI | AGGCCT | 1 cut(s) | 1100 |
| SsiI | CCGC | 2 cut(s) | 145, 154 |
| SspI | AATATT | 5 cut(s) | 816, 1080, 1153, 1223, 1323 |
| SspMI | CTAG | 2 cut(s) | 33, 372 |
| SstI | GAGCTC | 1 cut(s) | 471 |
| StuI | AGGCCT | 1 cut(s) | 1100 |
| StyD4I | CCNGG | 2 cut(s) | 927, 928 |
| StyI | CCWWGG | 2 cut(s) | 32, 521 |
| TaaI | ACNGT | 3 cut(s) | 57, 656, 1045 |
| TaiI | ACGT | 3 cut(s) | 268, 280, 544 |
| TaqI | TCGA | 3 cut(s) | 607, 634, 691 |
| TfiI | GAWTC | 3 cut(s) | 99, 224, 1402 |
| Tru1I | TTAA | 6 cut(s) | 86, 368, 620, 968, 1116, 1325 |
| Tru9I | TTAA | 6 cut(s) | 86, 368, 620, 968, 1116, 1325 |
| TscAI | CASTG | 2 cut(s) | 60, 335 |
| TseFI | GTSAC | 2 cut(s) | 838, 1145 |
| TseI | GCWGC | 5 cut(s) | 77, 156, 178, 895, 1037 |
| Tsp45I | GTSAC | 2 cut(s) | 838, 1145 |
| TspDTI | ATGAA | 5 cut(s) | 351, 546, 848, 950, 1188 |
| TspGWI | ACGGA | 2 cut(s) | 1080, 1086 |
| TspMI | CCCGGG | 1 cut(s) | 928 |
| TspRI | CASTG | 2 cut(s) | 60, 335 |
| Tth111I | GACNNNGTC | 1 cut(s) | 561 |
| VneI | GTGCAC | 1 cut(s) | 1427 |
| VpaK11BI | GGWCC | 2 cut(s) | 26, 151 |
| XapI | RAATTY | 2 cut(s) | 446, 1344 |
| XceI | RCATGY | 1 cut(s) | 554 |
| XhoI | CTCGAG | 1 cut(s) | 606 |
| XmaI | CCCGGG | 1 cut(s) | 928 |
| XmaJI | CCTAGG | 1 cut(s) | 32 |
| XmiI | GTMKAC | 1 cut(s) | 65 |
| XspI | CTAG | 2 cut(s) | 33, 372 |
| Zsp2I | ATGCAT | 2 cut(s) | 914, 999 |