Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 67, 77 |
| AccBSI | CCGCTC | 2 cut(s) | 166, 632 |
| AccII | CGCG | 2 cut(s) | 597, 781 |
| AciI | CCGC | 7 cut(s) | 166, 228, 591, 597, 600, 632, 677 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AcuI | CTGAAG | 2 cut(s) | 207, 693 |
| AcyI | GRCGYC | 2 cut(s) | 64, 74 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 380 |
| AflII | CTTAAG | 1 cut(s) | 355 |
| AgsI | TTSAA | 5 cut(s) | 112, 247, 482, 536, 685 |
| AjiI | CACGTC | 1 cut(s) | 85 |
| AjnI | CCWGG | 1 cut(s) | 385 |
| AluBI | AGCT | 2 cut(s) | 115, 143 |
| AluI | AGCT | 2 cut(s) | 115, 143 |
| Alw26I | GTCTC | 1 cut(s) | 796 |
| Ama87I | CYCGRG | 1 cut(s) | 197 |
| AoxI | GGCC | 5 cut(s) | 301, 345, 359, 383, 712 |
| ApeKI | GCWGC | 2 cut(s) | 268, 771 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| AseI | ATTAAT | 1 cut(s) | 207 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 746 |
| AspLEI | GCGC | 1 cut(s) | 771 |
| AspS9I | GGNCC | 3 cut(s) | 301, 346, 383 |
| AsuHPI | GGTGA | 1 cut(s) | 533 |
| AvaI | CYCGRG | 1 cut(s) | 197 |
| BbvI | GCAGC | 2 cut(s) | 280, 783 |
| BccI | CCATC | 2 cut(s) | 4, 164 |
| BceAI | ACGGC | 1 cut(s) | 37 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 656, 690 |
| BciT130I | CCWGG | 1 cut(s) | 387 |
| BcoDI | GTCTC | 1 cut(s) | 796 |
| BfaI | CTAG | 6 cut(s) | 78, 224, 375, 405, 462, 693 |
| BfrI | CTTAAG | 1 cut(s) | 355 |
| BisI | GCNGC | 3 cut(s) | 269, 598, 772 |
| BlsI | GCNGC | 3 cut(s) | 270, 599, 773 |
| Bme1390I | CCNGG | 1 cut(s) | 387 |
| BmeT110I | CYCGRG | 1 cut(s) | 197 |
| BmgBI | CACGTC | 1 cut(s) | 85 |
| BmgT120I | GGNCC | 3 cut(s) | 301, 346, 383 |
| BmiI | GGNNCC | 1 cut(s) | 102 |
| BmrFI | CCNGG | 1 cut(s) | 387 |
| BmsI | GCATC | 2 cut(s) | 463, 664 |
| BoxI | GACNNNNGTC | 1 cut(s) | 582 |
| BsaHI | GRCGYC | 2 cut(s) | 64, 74 |
| BsaI | GGTCTC | 1 cut(s) | 796 |
| BsaJI | CCNNGG | 1 cut(s) | 715 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 380 |
| BseBI | CCWGG | 1 cut(s) | 387 |
| BseDI | CCNNGG | 1 cut(s) | 715 |
| BseGI | GGATG | 3 cut(s) | 175, 207, 679 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 380 |
| BseXI | GCAGC | 2 cut(s) | 280, 783 |
| Bsh1236I | CGCG | 2 cut(s) | 597, 781 |
| Bsh1285I | CGRYCG | 1 cut(s) | 595 |
| BshFI | GGCC | 5 cut(s) | 303, 347, 361, 385, 714 |
| BsiEI | CGRYCG | 1 cut(s) | 595 |
| BsiHKCI | CYCGRG | 1 cut(s) | 197 |
| BsiSI | CCGG | 1 cut(s) | 70 |
| BslFI | GGGAC | 1 cut(s) | 734 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 380 |
| BsmAI | GTCTC | 1 cut(s) | 796 |
| BsmFI | GGGAC | 1 cut(s) | 734 |
| BsmI | GAATGC | 2 cut(s) | 262, 559 |
| BsnI | GGCC | 5 cut(s) | 303, 347, 361, 385, 714 |
| Bso31I | GGTCTC | 1 cut(s) | 796 |
| BsoBI | CYCGRG | 1 cut(s) | 197 |
| Bsp143I | GATC | 3 cut(s) | 464, 547, 776 |
| Bsp19I | CCATGG | 1 cut(s) | 715 |
| BspACI | CCGC | 7 cut(s) | 166, 228, 591, 597, 600, 632, 677 |
| BspANI | GGCC | 5 cut(s) | 303, 347, 361, 385, 714 |
| BspFNI | CGCG | 2 cut(s) | 597, 781 |
| BspLI | GGNNCC | 1 cut(s) | 102 |
| BspTI | CTTAAG | 1 cut(s) | 355 |
| BspTNI | GGTCTC | 1 cut(s) | 796 |
| BsrBI | CCGCTC | 2 cut(s) | 166, 632 |
| BssECI | CCNNGG | 1 cut(s) | 715 |
| BssMI | GATC | 3 cut(s) | 464, 547, 776 |
| BssNI | GRCGYC | 2 cut(s) | 64, 74 |
| BssT1I | CCWWGG | 1 cut(s) | 715 |
| Bst2UI | CCWGG | 1 cut(s) | 387 |
| Bst6I | CTCTTC | 1 cut(s) | 680 |
| BstACI | GRCGYC | 2 cut(s) | 64, 74 |
| BstAFI | CTTAAG | 1 cut(s) | 355 |
| BstDEI | CTNAG | 2 cut(s) | 116, 369 |
| BstDSI | CCRYGG | 1 cut(s) | 715 |
| BstF5I | GGATG | 3 cut(s) | 175, 207, 679 |
| BstFNI | CGCG | 2 cut(s) | 597, 781 |
| BstHHI | GCGC | 1 cut(s) | 771 |
| BstKTI | GATC | 3 cut(s) | 467, 550, 779 |
| BstMAI | GTCTC | 1 cut(s) | 796 |
| BstMBI | GATC | 3 cut(s) | 464, 547, 776 |
| BstMCI | CGRYCG | 1 cut(s) | 595 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 597, 603 |
| BstNI | CCWGG | 1 cut(s) | 387 |
| BstNSI | RCATGY | 1 cut(s) | 264 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 582 |
| BstSCI | CCNGG | 1 cut(s) | 385 |
| BstUI | CGCG | 2 cut(s) | 597, 781 |
| BstV1I | GCAGC | 2 cut(s) | 280, 783 |
| BsuRI | GGCC | 5 cut(s) | 303, 347, 361, 385, 714 |
| BtgI | CCRYGG | 1 cut(s) | 715 |
| BtgZI | GCGATG | 1 cut(s) | 633 |
| BtrI | CACGTC | 1 cut(s) | 85 |
| BtsCI | GGATG | 3 cut(s) | 175, 207, 679 |
| BtsIMutI | CAGTG | 1 cut(s) | 331 |
| CfoI | GCGC | 1 cut(s) | 771 |
| Cfr13I | GGNCC | 3 cut(s) | 301, 346, 383 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 307, 342 |
| CviAII | CATG | 6 cut(s) | 261, 350, 575, 587, 617, 716 |
| DdeI | CTNAG | 2 cut(s) | 116, 369 |
| DpnI | GATC | 3 cut(s) | 466, 549, 778 |
| DpnII | GATC | 3 cut(s) | 464, 547, 776 |
| Eam1104I | CTCTTC | 1 cut(s) | 680 |
| EarI | CTCTTC | 1 cut(s) | 680 |
| EciI | GGCGGA | 1 cut(s) | 615 |
| Eco130I | CCWWGG | 1 cut(s) | 715 |
| Eco31I | GGTCTC | 1 cut(s) | 796 |
| Eco57I | CTGAAG | 2 cut(s) | 207, 693 |
| Eco88I | CYCGRG | 1 cut(s) | 197 |
| EcoRII | CCWGG | 1 cut(s) | 385 |
| EcoT14I | CCWWGG | 1 cut(s) | 715 |
| EcoT22I | ATGCAT | 1 cut(s) | 262 |
| ErhI | CCWWGG | 1 cut(s) | 715 |
| FaeI | CATG | 6 cut(s) | 264, 353, 578, 590, 620, 719 |
| FaiI | YATR | 8 cut(s) | 262, 284, 351, 449, 576, 588, 618, 717 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 734, 766 |
| FaqI | GGGAC | 1 cut(s) | 734 |
| FatI | CATG | 6 cut(s) | 260, 349, 574, 586, 616, 715 |
| FauI | CCCGC | 4 cut(s) | 159, 221, 625, 670 |
| Fnu4HI | GCNGC | 3 cut(s) | 269, 598, 772 |
| FokI | GGATG | 3 cut(s) | 182, 214, 686 |
| Fsp4HI | GCNGC | 3 cut(s) | 269, 598, 772 |
| FspBI | CTAG | 6 cut(s) | 78, 224, 375, 405, 462, 693 |
| GlaI | GCGC | 1 cut(s) | 770 |
| GluI | GCNGC | 3 cut(s) | 269, 598, 772 |
| HaeIII | GGCC | 5 cut(s) | 303, 347, 361, 385, 714 |
| HapII | CCGG | 1 cut(s) | 70 |
| HhaI | GCGC | 1 cut(s) | 771 |
| Hin1I | GRCGYC | 2 cut(s) | 64, 74 |
| Hin1II | CATG | 6 cut(s) | 264, 353, 578, 590, 620, 719 |
| Hin6I | GCGC | 1 cut(s) | 769 |
| HinP1I | GCGC | 1 cut(s) | 769 |
| HindIII | AAGCTT | 1 cut(s) | 113 |
| HinfI | GANTC | 2 cut(s) | 35, 401 |
| HpaII | CCGG | 1 cut(s) | 70 |
| HphI | GGTGA | 1 cut(s) | 533 |
| Hpy166II | GTNNAC | 1 cut(s) | 19 |
| Hpy188I | TCNGA | 4 cut(s) | 187, 255, 400, 552 |
| Hpy188III | TCNNGA | 3 cut(s) | 199, 405, 693 |
| Hpy8I | GTNNAC | 1 cut(s) | 19 |
| Hpy99I | CGWCG | 3 cut(s) | 29, 66, 69 |
| HpyAV | CCTTC | 7 cut(s) | 25, 206, 226, 505, 717, 732, 736 |
| HpyCH4IV | ACGT | 3 cut(s) | 64, 74, 84 |
| HpyCH4V | TGCA | 1 cut(s) | 260 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 597, 603 |
| HpyF3I | CTNAG | 2 cut(s) | 116, 369 |
| HpySE526I | ACGT | 3 cut(s) | 64, 74, 84 |
| Hsp92I | GRCGYC | 2 cut(s) | 64, 74 |
| Hsp92II | CATG | 6 cut(s) | 264, 353, 578, 590, 620, 719 |
| HspAI | GCGC | 1 cut(s) | 769 |
| Kzo9I | GATC | 3 cut(s) | 464, 547, 776 |
| LmnI | GCTCC | 2 cut(s) | 100, 140 |
| LpnPI | CCDG | 5 cut(s) | 83, 329, 372, 399, 696 |
| Lsp1109I | GCAGC | 2 cut(s) | 280, 783 |
| LweI | GCATC | 2 cut(s) | 463, 664 |
| MaeI | CTAG | 6 cut(s) | 78, 224, 375, 405, 462, 693 |
| MaeII | ACGT | 3 cut(s) | 64, 74, 84 |
| MaeIII | GTNAC | 1 cut(s) | 80 |
| MalI | GATC | 3 cut(s) | 466, 549, 778 |
| MbiI | CCGCTC | 2 cut(s) | 166, 632 |
| MboI | GATC | 3 cut(s) | 464, 547, 776 |
| MboII | GAAGA | 3 cut(s) | 557, 649, 697 |
| MfeI | CAATTG | 1 cut(s) | 50 |
| MluCI | AATT | 5 cut(s) | 50, 94, 107, 442, 482 |
| MlyI | GAGTC | 1 cut(s) | 410 |
| MmeI | TCCRAC | 2 cut(s) | 651, 736 |
| Mph1103I | ATGCAT | 1 cut(s) | 262 |
| MroXI | GAANNNNTTC | 1 cut(s) | 746 |
| MseI | TTAA | 4 cut(s) | 125, 207, 356, 799 |
| MslI | CAYNNNNRTG | 1 cut(s) | 621 |
| MspCI | CTTAAG | 1 cut(s) | 355 |
| MspI | CCGG | 1 cut(s) | 70 |
| MspR9I | CCNGG | 1 cut(s) | 387 |
| MunI | CAATTG | 1 cut(s) | 50 |
| Mva1269I | GAATGC | 2 cut(s) | 262, 559 |
| MvaI | CCWGG | 1 cut(s) | 387 |
| MvnI | CGCG | 2 cut(s) | 597, 781 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 597, 603 |
| NcoI | CCATGG | 1 cut(s) | 715 |
| NdeII | GATC | 3 cut(s) | 464, 547, 776 |
| NlaIII | CATG | 6 cut(s) | 264, 353, 578, 590, 620, 719 |
| NlaIV | GGNNCC | 1 cut(s) | 102 |
| NmuCI | GTSAC | 1 cut(s) | 80 |
| NsiI | ATGCAT | 1 cut(s) | 262 |
| NspI | RCATGY | 1 cut(s) | 264 |
| PctI | GAATGC | 2 cut(s) | 262, 559 |
| PdmI | GAANNNNTTC | 1 cut(s) | 746 |
| PfeI | GAWTC | 1 cut(s) | 35 |
| PkrI | GCNGC | 3 cut(s) | 270, 599, 773 |
| PleI | GAGTC | 1 cut(s) | 409 |
| PpsI | GAGTC | 1 cut(s) | 409 |
| PshAI | GACNNNNGTC | 1 cut(s) | 582 |
| PshBI | ATTAAT | 1 cut(s) | 207 |
| Psp6I | CCWGG | 1 cut(s) | 385 |
| PspGI | CCWGG | 1 cut(s) | 385 |
| PspN4I | GGNNCC | 1 cut(s) | 102 |
| PspPI | GGNCC | 3 cut(s) | 301, 346, 383 |
| RseI | CAYNNNNRTG | 1 cut(s) | 621 |
| SaqAI | TTAA | 4 cut(s) | 125, 207, 356, 799 |
| SatI | GCNGC | 3 cut(s) | 269, 598, 772 |
| Sau3AI | GATC | 3 cut(s) | 464, 547, 776 |
| Sau96I | GGNCC | 3 cut(s) | 301, 346, 383 |
| SchI | GAGTC | 1 cut(s) | 410 |
| ScrFI | CCNGG | 1 cut(s) | 387 |
| SfaNI | GCATC | 2 cut(s) | 463, 664 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 621 |
| SmlI | CTYRAG | 1 cut(s) | 355 |
| SmoI | CTYRAG | 1 cut(s) | 355 |
| Sse9I | AATT | 5 cut(s) | 50, 94, 107, 442, 482 |
| SsiI | CCGC | 7 cut(s) | 166, 228, 591, 597, 600, 632, 677 |
| SspI | AATATT | 2 cut(s) | 156, 297 |
| SspMI | CTAG | 6 cut(s) | 78, 224, 375, 405, 462, 693 |
| StyD4I | CCNGG | 1 cut(s) | 385 |
| StyI | CCWWGG | 1 cut(s) | 715 |
| TaiI | ACGT | 3 cut(s) | 67, 77, 87 |
| TaqI | TCGA | 3 cut(s) | 38, 503, 763 |
| TaqII | GACCGA | 1 cut(s) | 778 |
| TasI | AATT | 5 cut(s) | 50, 94, 107, 442, 482 |
| TauI | GCSGC | 1 cut(s) | 600 |
| TfiI | GAWTC | 1 cut(s) | 35 |
| Tru1I | TTAA | 4 cut(s) | 125, 207, 356, 799 |
| Tru9I | TTAA | 4 cut(s) | 125, 207, 356, 799 |
| TscAI | CASTG | 1 cut(s) | 331 |
| TseFI | GTSAC | 1 cut(s) | 80 |
| TseI | GCWGC | 2 cut(s) | 268, 771 |
| Tsp45I | GTSAC | 1 cut(s) | 80 |
| TspDTI | ATGAA | 1 cut(s) | 225 |
| TspGWI | ACGGA | 1 cut(s) | 594 |
| TspRI | CASTG | 1 cut(s) | 331 |
| Vha464I | CTTAAG | 1 cut(s) | 355 |
| VspI | ATTAAT | 1 cut(s) | 207 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XbaI | TCTAGA | 2 cut(s) | 404, 692 |
| XceI | RCATGY | 1 cut(s) | 264 |
| XmnI | GAANNNNTTC | 1 cut(s) | 746 |
| XspI | CTAG | 6 cut(s) | 78, 224, 375, 405, 462, 693 |
| ZraI | GACGTC | 2 cut(s) | 65, 75 |
| Zsp2I | ATGCAT | 1 cut(s) | 262 |