Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 371 |
| AccB1I | GGYRCC | 2 cut(s) | 83, 269 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 425 |
| AccBSI | CCGCTC | 2 cut(s) | 215, 442 |
| AccII | CGCG | 3 cut(s) | 169, 355, 377 |
| AciI | CCGC | 8 cut(s) | 88, 159, 167, 213, 323, 377, 440, 471 |
| AclWI | GGATC | 3 cut(s) | 905, 907, 920 |
| AcoI | YGGCCR | 3 cut(s) | 302, 499, 793 |
| AcsI | RAATTY | 1 cut(s) | 722 |
| AcyI | GRCGYC | 1 cut(s) | 189 |
| AdeI | CACNNNGTG | 1 cut(s) | 425 |
| AfaI | GTAC | 3 cut(s) | 710, 736, 848 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 16, 66, 67, 219, 425, 554, 648, 824 |
| AflIII | ACRYGT | 1 cut(s) | 686 |
| AgsI | TTSAA | 1 cut(s) | 259 |
| AjiI | CACGTC | 1 cut(s) | 148 |
| AjnI | CCWGG | 1 cut(s) | 849 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 909, 941 |
| AluBI | AGCT | 2 cut(s) | 287, 328 |
| AluI | AGCT | 2 cut(s) | 287, 328 |
| Alw21I | GWGCWC | 1 cut(s) | 678 |
| Alw26I | GTCTC | 2 cut(s) | 165, 866 |
| AlwI | GGATC | 3 cut(s) | 905, 907, 920 |
| Ama87I | CYCGRG | 1 cut(s) | 248 |
| ApoI | RAATTY | 1 cut(s) | 722 |
| AspLEI | GCGC | 1 cut(s) | 166 |
| AspS9I | GGNCC | 6 cut(s) | 117, 183, 222, 610, 781, 887 |
| AsuC2I | CCSGG | 3 cut(s) | 249, 250, 671 |
| AsuHPI | GGTGA | 5 cut(s) | 429, 505, 523, 583, 589 |
| AvaI | CYCGRG | 1 cut(s) | 248 |
| BaeGI | GKGCMC | 3 cut(s) | 88, 274, 300 |
| BalI | TGGCCA | 2 cut(s) | 304, 501 |
| BamHI | GGATCC | 1 cut(s) | 912 |
| BanI | GGYRCC | 2 cut(s) | 83, 269 |
| BanII | GRGCYC | 1 cut(s) | 832 |
| BbsI | GAAGAC | 2 cut(s) | 89, 407 |
| Bbv12I | GWGCWC | 1 cut(s) | 678 |
| BccI | CCATC | 9 cut(s) | 150, 200, 313, 320, 542, 548, 761, 772, 970 |
| BceAI | ACGGC | 1 cut(s) | 808 |
| BciT130I | CCWGG | 1 cut(s) | 851 |
| BclI | TGATCA | 1 cut(s) | 970 |
| BcnI | CCSGG | 3 cut(s) | 249, 250, 671 |
| BcoDI | GTCTC | 2 cut(s) | 165, 866 |
| BfaI | CTAG | 2 cut(s) | 75, 155 |
| BfoI | RGCGCY | 1 cut(s) | 167 |
| BfuAI | ACCTGC | 1 cut(s) | 371 |
| BisI | GCNGC | 2 cut(s) | 167, 378 |
| BlsI | GCNGC | 2 cut(s) | 168, 379 |
| BmcAI | AGTACT | 1 cut(s) | 710 |
| Bme1390I | CCNGG | 4 cut(s) | 249, 250, 671, 851 |
| BmeT110I | CYCGRG | 1 cut(s) | 248 |
| BmgBI | CACGTC | 1 cut(s) | 148 |
| BmgT120I | GGNCC | 6 cut(s) | 117, 183, 222, 610, 781, 887 |
| BmiI | GGNNCC | 7 cut(s) | 6, 85, 271, 640, 829, 889, 914 |
| BmrFI | CCNGG | 4 cut(s) | 249, 250, 671, 851 |
| BmsI | GCATC | 3 cut(s) | 87, 871, 888 |
| BpiI | GAAGAC | 2 cut(s) | 89, 407 |
| BpuMI | CCSGG | 3 cut(s) | 249, 250, 671 |
| Bsa29I | ATCGAT | 2 cut(s) | 27, 399 |
| BsaHI | GRCGYC | 1 cut(s) | 189 |
| BsaJI | CCNNGG | 4 cut(s) | 248, 249, 606, 647 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 16, 66, 67, 219, 425, 554, 648, 824 |
| Bse1I | ACTGG | 4 cut(s) | 230, 305, 544, 716 |
| Bse3DI | GCAATG | 1 cut(s) | 631 |
| BseBI | CCWGG | 1 cut(s) | 851 |
| BseCI | ATCGAT | 2 cut(s) | 27, 399 |
| BseDI | CCNNGG | 4 cut(s) | 248, 249, 606, 647 |
| BseGI | GGATG | 2 cut(s) | 305, 948 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 16, 66, 67, 219, 425, 554, 648, 824 |
| BseMI | GCAATG | 1 cut(s) | 631 |
| BseNI | ACTGG | 4 cut(s) | 230, 305, 544, 716 |
| BseRI | GAGGAG | 1 cut(s) | 116 |
| BseSI | GKGCMC | 3 cut(s) | 88, 274, 300 |
| BseYI | CCCAGC | 1 cut(s) | 319 |
| Bsh1236I | CGCG | 3 cut(s) | 169, 355, 377 |
| BshNI | GGYRCC | 2 cut(s) | 83, 269 |
| BshVI | ATCGAT | 2 cut(s) | 27, 399 |
| BsiHKAI | GWGCWC | 1 cut(s) | 678 |
| BsiHKCI | CYCGRG | 1 cut(s) | 248 |
| BsiSI | CCGG | 2 cut(s) | 249, 670 |
| BslFI | GGGAC | 1 cut(s) | 266 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 16, 66, 67, 219, 425, 554, 648, 824 |
| BsmAI | GTCTC | 2 cut(s) | 165, 866 |
| BsmFI | GGGAC | 1 cut(s) | 266 |
| BsoBI | CYCGRG | 1 cut(s) | 248 |
| Bsp1286I | GDGCHC | 5 cut(s) | 88, 274, 300, 678, 832 |
| Bsp143I | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| Bsp19I | CCATGG | 1 cut(s) | 606 |
| BspACI | CCGC | 8 cut(s) | 88, 159, 167, 213, 323, 377, 440, 471 |
| BspDI | ATCGAT | 2 cut(s) | 27, 399 |
| BspFNI | CGCG | 3 cut(s) | 169, 355, 377 |
| BspLI | GGNNCC | 7 cut(s) | 6, 85, 271, 640, 829, 889, 914 |
| BspMI | ACCTGC | 1 cut(s) | 371 |
| BspPI | GGATC | 3 cut(s) | 905, 907, 920 |
| BspT107I | GGYRCC | 2 cut(s) | 83, 269 |
| BsrBI | CCGCTC | 2 cut(s) | 215, 442 |
| BsrDI | GCAATG | 1 cut(s) | 631 |
| BsrI | ACTGG | 4 cut(s) | 230, 305, 544, 716 |
| BssECI | CCNNGG | 4 cut(s) | 248, 249, 606, 647 |
| BssMI | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| BssNI | GRCGYC | 1 cut(s) | 189 |
| BssT1I | CCWWGG | 1 cut(s) | 606 |
| Bst2UI | CCWGG | 1 cut(s) | 851 |
| Bst4CI | ACNGT | 3 cut(s) | 635, 651, 800 |
| Bst6I | CTCTTC | 1 cut(s) | 774 |
| BstACI | GRCGYC | 1 cut(s) | 189 |
| BstC8I | GCNNGC | 4 cut(s) | 88, 213, 444, 560 |
| BstDSI | CCRYGG | 2 cut(s) | 606, 647 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 822 |
| BstF5I | GGATG | 2 cut(s) | 305, 948 |
| BstFNI | CGCG | 3 cut(s) | 169, 355, 377 |
| BstH2I | RGCGCY | 1 cut(s) | 167 |
| BstHHI | GCGC | 1 cut(s) | 166 |
| BstKTI | GATC | 5 cut(s) | 31, 403, 900, 915, 973 |
| BstMAI | GTCTC | 2 cut(s) | 165, 866 |
| BstMBI | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 203, 212, 568, 885 |
| BstNI | CCWGG | 1 cut(s) | 851 |
| BstNSI | RCATGY | 1 cut(s) | 690 |
| BstSCI | CCNGG | 4 cut(s) | 247, 248, 669, 849 |
| BstSLI | GKGCMC | 3 cut(s) | 88, 274, 300 |
| BstUI | CGCG | 3 cut(s) | 169, 355, 377 |
| BstV2I | GAAGAC | 2 cut(s) | 89, 407 |
| BstX2I | RGATCY | 2 cut(s) | 897, 912 |
| BstYI | RGATCY | 2 cut(s) | 897, 912 |
| Bsu15I | ATCGAT | 2 cut(s) | 27, 399 |
| BsuTUI | ATCGAT | 2 cut(s) | 27, 399 |
| BtgI | CCRYGG | 2 cut(s) | 606, 647 |
| BtgZI | GCGATG | 2 cut(s) | 178, 791 |
| BtrI | CACGTC | 1 cut(s) | 148 |
| BtsCI | GGATG | 2 cut(s) | 305, 948 |
| BtsI | GCAGTG | 1 cut(s) | 430 |
| BtsIMutI | CAGTG | 5 cut(s) | 298, 430, 537, 640, 805 |
| BveI | ACCTGC | 1 cut(s) | 371 |
| Cac8I | GCNNGC | 4 cut(s) | 88, 213, 444, 560 |
| CfoI | GCGC | 1 cut(s) | 166 |
| Cfr13I | GGNCC | 6 cut(s) | 117, 183, 222, 610, 781, 887 |
| Cfr9I | CCCGGG | 1 cut(s) | 248 |
| ClaI | ATCGAT | 2 cut(s) | 27, 399 |
| CseI | GACGC | 1 cut(s) | 197 |
| Csp6I | GTAC | 3 cut(s) | 709, 735, 847 |
| CviAII | CATG | 4 cut(s) | 572, 607, 617, 687 |
| CviQI | GTAC | 3 cut(s) | 709, 735, 847 |
| DpnI | GATC | 5 cut(s) | 30, 402, 899, 914, 972 |
| DpnII | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| DraIII | CACNNNGTG | 1 cut(s) | 425 |
| EaeI | YGGCCR | 3 cut(s) | 302, 499, 793 |
| Eam1104I | CTCTTC | 1 cut(s) | 774 |
| EarI | CTCTTC | 1 cut(s) | 774 |
| EciI | GGCGGA | 1 cut(s) | 174 |
| Eco130I | CCWWGG | 1 cut(s) | 606 |
| Eco24I | GRGCYC | 1 cut(s) | 832 |
| Eco88I | CYCGRG | 1 cut(s) | 248 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 822 |
| EcoO109I | RGGNCCY | 1 cut(s) | 117 |
| EcoRII | CCWGG | 1 cut(s) | 849 |
| EcoT14I | CCWWGG | 1 cut(s) | 606 |
| EcoT22I | ATGCAT | 1 cut(s) | 864 |
| EcoT38I | GRGCYC | 1 cut(s) | 832 |
| ErhI | CCWWGG | 1 cut(s) | 606 |
| FaeI | CATG | 4 cut(s) | 575, 610, 620, 690 |
| FaiI | YATR | 9 cut(s) | 63, 114, 339, 393, 573, 608, 618, 688, 860 |
| FaqI | GGGAC | 1 cut(s) | 266 |
| FatI | CATG | 4 cut(s) | 571, 606, 616, 686 |
| FauI | CCCGC | 2 cut(s) | 95, 220 |
| FbaI | TGATCA | 1 cut(s) | 970 |
| Fnu4HI | GCNGC | 2 cut(s) | 167, 378 |
| FokI | GGATG | 2 cut(s) | 292, 935 |
| FriOI | GRGCYC | 1 cut(s) | 832 |
| Fsp4HI | GCNGC | 2 cut(s) | 167, 378 |
| FspBI | CTAG | 2 cut(s) | 75, 155 |
| GlaI | GCGC | 1 cut(s) | 165 |
| GluI | GCNGC | 2 cut(s) | 167, 378 |
| GsaI | CCCAGC | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 167 |
| HapII | CCGG | 2 cut(s) | 249, 670 |
| HgaI | GACGC | 1 cut(s) | 197 |
| HhaI | GCGC | 1 cut(s) | 166 |
| Hin1I | GRCGYC | 1 cut(s) | 189 |
| Hin1II | CATG | 4 cut(s) | 575, 610, 620, 690 |
| Hin6I | GCGC | 1 cut(s) | 164 |
| HinP1I | GCGC | 1 cut(s) | 164 |
| HinfI | GANTC | 6 cut(s) | 173, 244, 277, 584, 693, 766 |
| HpaII | CCGG | 2 cut(s) | 249, 670 |
| HphI | GGTGA | 5 cut(s) | 429, 505, 523, 583, 589 |
| Hpy166II | GTNNAC | 1 cut(s) | 437 |
| Hpy188I | TCNGA | 2 cut(s) | 152, 239 |
| Hpy188III | TCNNGA | 4 cut(s) | 32, 370, 910, 941 |
| Hpy8I | GTNNAC | 1 cut(s) | 437 |
| Hpy99I | CGWCG | 4 cut(s) | 152, 191, 222, 942 |
| HpyAV | CCTTC | 4 cut(s) | 10, 234, 493, 604 |
| HpyCH4III | ACNGT | 3 cut(s) | 635, 651, 800 |
| HpyCH4IV | ACGT | 1 cut(s) | 147 |
| HpyCH4V | TGCA | 2 cut(s) | 571, 862 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 203, 212, 568, 885 |
| HpySE526I | ACGT | 1 cut(s) | 147 |
| Hsp92I | GRCGYC | 1 cut(s) | 189 |
| Hsp92II | CATG | 4 cut(s) | 575, 610, 620, 690 |
| HspAI | GCGC | 1 cut(s) | 164 |
| Ksp22I | TGATCA | 1 cut(s) | 970 |
| Kzo9I | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| LmnI | GCTCC | 6 cut(s) | 10, 161, 325, 644, 673, 827 |
| LweI | GCATC | 3 cut(s) | 87, 871, 888 |
| MaeI | CTAG | 2 cut(s) | 75, 155 |
| MaeII | ACGT | 1 cut(s) | 147 |
| MaeIII | GTNAC | 1 cut(s) | 589 |
| MalI | GATC | 5 cut(s) | 30, 402, 899, 914, 972 |
| MbiI | CCGCTC | 2 cut(s) | 215, 442 |
| MboI | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| MboII | GAAGA | 6 cut(s) | 47, 50, 89, 407, 721, 761 |
| MflI | RGATCY | 2 cut(s) | 897, 912 |
| MhlI | GDGCHC | 5 cut(s) | 88, 274, 300, 678, 832 |
| MlsI | TGGCCA | 2 cut(s) | 304, 501 |
| MluCI | AATT | 3 cut(s) | 131, 578, 722 |
| MluNI | TGGCCA | 2 cut(s) | 304, 501 |
| MlyI | GAGTC | 4 cut(s) | 167, 238, 578, 760 |
| MmeI | TCCRAC | 2 cut(s) | 130, 501 |
| MnlI | CCTC | 7 cut(s) | 115, 137, 245, 319, 610, 751, 794 |
| Mox20I | TGGCCA | 2 cut(s) | 304, 501 |
| Mph1103I | ATGCAT | 1 cut(s) | 864 |
| MscI | TGGCCA | 2 cut(s) | 304, 501 |
| Msp20I | TGGCCA | 2 cut(s) | 304, 501 |
| MspA1I | CMGCKG | 1 cut(s) | 323 |
| MspI | CCGG | 2 cut(s) | 249, 670 |
| MspR9I | CCNGG | 4 cut(s) | 249, 250, 671, 851 |
| MvaI | CCWGG | 1 cut(s) | 851 |
| MvnI | CGCG | 3 cut(s) | 169, 355, 377 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 203, 212, 568, 885 |
| NciI | CCSGG | 3 cut(s) | 249, 250, 671 |
| NcoI | CCATGG | 1 cut(s) | 606 |
| NdeII | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| NlaIII | CATG | 4 cut(s) | 575, 610, 620, 690 |
| NlaIV | GGNNCC | 7 cut(s) | 6, 85, 271, 640, 829, 889, 914 |
| NmuCI | GTSAC | 1 cut(s) | 589 |
| NsiI | ATGCAT | 1 cut(s) | 864 |
| NspI | RCATGY | 1 cut(s) | 690 |
| PciI | ACATGT | 1 cut(s) | 686 |
| PfeI | GAWTC | 2 cut(s) | 277, 693 |
| PflFI | GACNNNGTC | 1 cut(s) | 587 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 425 |
| PkrI | GCNGC | 2 cut(s) | 168, 379 |
| PleI | GAGTC | 4 cut(s) | 167, 238, 578, 760 |
| PpsI | GAGTC | 4 cut(s) | 167, 238, 578, 760 |
| PscI | ACATGT | 1 cut(s) | 686 |
| Psp6I | CCWGG | 1 cut(s) | 849 |
| PspFI | CCCAGC | 1 cut(s) | 319 |
| PspGI | CCWGG | 1 cut(s) | 849 |
| PspN4I | GGNNCC | 7 cut(s) | 6, 85, 271, 640, 829, 889, 914 |
| PspPI | GGNCC | 6 cut(s) | 117, 183, 222, 610, 781, 887 |
| PsuI | RGATCY | 2 cut(s) | 897, 912 |
| PsyI | GACNNNGTC | 1 cut(s) | 587 |
| RsaI | GTAC | 3 cut(s) | 710, 736, 848 |
| RsaNI | GTAC | 3 cut(s) | 709, 735, 847 |
| SatI | GCNGC | 2 cut(s) | 167, 378 |
| Sau3AI | GATC | 5 cut(s) | 28, 400, 897, 912, 970 |
| Sau96I | GGNCC | 6 cut(s) | 117, 183, 222, 610, 781, 887 |
| ScaI | AGTACT | 1 cut(s) | 710 |
| SchI | GAGTC | 4 cut(s) | 167, 238, 578, 760 |
| ScrFI | CCNGG | 4 cut(s) | 249, 250, 671, 851 |
| SduI | GDGCHC | 5 cut(s) | 88, 274, 300, 678, 832 |
| SfaNI | GCATC | 3 cut(s) | 87, 871, 888 |
| SmaI | CCCGGG | 1 cut(s) | 250 |
| Sse9I | AATT | 3 cut(s) | 131, 578, 722 |
| SsiI | CCGC | 8 cut(s) | 88, 159, 167, 213, 323, 377, 440, 471 |
| SspMI | CTAG | 2 cut(s) | 75, 155 |
| StyD4I | CCNGG | 4 cut(s) | 247, 248, 669, 849 |
| StyI | CCWWGG | 1 cut(s) | 606 |
| TaaI | ACNGT | 3 cut(s) | 635, 651, 800 |
| TaiI | ACGT | 1 cut(s) | 150 |
| TaqI | TCGA | 6 cut(s) | 27, 283, 399, 486, 493, 602 |
| TasI | AATT | 3 cut(s) | 131, 578, 722 |
| TatI | WGTACW | 2 cut(s) | 708, 734 |
| TauI | GCSGC | 2 cut(s) | 169, 380 |
| TfiI | GAWTC | 2 cut(s) | 277, 693 |
| TscAI | CASTG | 5 cut(s) | 305, 430, 544, 640, 805 |
| TseFI | GTSAC | 1 cut(s) | 589 |
| Tsp45I | GTSAC | 1 cut(s) | 589 |
| TspDTI | ATGAA | 2 cut(s) | 63, 715 |
| TspMI | CCCGGG | 1 cut(s) | 248 |
| TspRI | CASTG | 5 cut(s) | 305, 430, 544, 640, 805 |
| Tth111I | GACNNNGTC | 1 cut(s) | 587 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 425 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 822 |
| XapI | RAATTY | 1 cut(s) | 722 |
| XceI | RCATGY | 1 cut(s) | 690 |
| XmaI | CCCGGG | 1 cut(s) | 248 |
| XspI | CTAG | 2 cut(s) | 75, 155 |
| ZrmI | AGTACT | 1 cut(s) | 710 |
| Zsp2I | ATGCAT | 1 cut(s) | 864 |