Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1033 |
| AccB1I | GGYRCC | 4 cut(s) | 71, 656, 725, 1033 |
| AccI | GTMKAC | 1 cut(s) | 1222 |
| AccII | CGCG | 3 cut(s) | 321, 933, 1215 |
| AccIII | TCCGGA | 1 cut(s) | 954 |
| AciI | CCGC | 9 cut(s) | 167, 261, 321, 369, 694, 928, 931, 963, 1009 |
| AclWI | GGATC | 3 cut(s) | 770, 887, 965 |
| AcoI | YGGCCR | 1 cut(s) | 691 |
| AcsI | RAATTY | 1 cut(s) | 620 |
| AcuI | CTGAAG | 2 cut(s) | 373, 1040 |
| AcyI | GRCGYC | 1 cut(s) | 726 |
| AfaI | GTAC | 4 cut(s) | 203, 867, 1035, 1208 |
| AfeI | AGCGCT | 1 cut(s) | 526 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 323, 452, 722, 785, 1043, 1185 |
| AgeI | ACCGGT | 1 cut(s) | 715 |
| AgsI | TTSAA | 2 cut(s) | 46, 605 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 716 |
| AjnI | CCWGG | 7 cut(s) | 65, 659, 720, 779, 886, 918, 1161 |
| AluBI | AGCT | 3 cut(s) | 978, 985, 1060 |
| AluI | AGCT | 3 cut(s) | 978, 985, 1060 |
| Alw21I | GWGCWC | 2 cut(s) | 88, 987 |
| Alw26I | GTCTC | 2 cut(s) | 95, 246 |
| AlwI | GGATC | 3 cut(s) | 770, 887, 965 |
| Aor13HI | TCCGGA | 1 cut(s) | 954 |
| Aor51HI | AGCGCT | 1 cut(s) | 526 |
| AoxI | GGCC | 9 cut(s) | 157, 221, 439, 507, 678, 691, 1018, 1188, 1237 |
| ApaI | GGGCCC | 2 cut(s) | 1192, 1241 |
| ApeKI | GCWGC | 4 cut(s) | 56, 344, 522, 1060 |
| ApoI | RAATTY | 1 cut(s) | 620 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 377, 409, 1160, 1192 |
| AsiGI | ACCGGT | 1 cut(s) | 715 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1115 |
| Asp718I | GGTACC | 1 cut(s) | 1033 |
| AspLEI | GCGC | 5 cut(s) | 527, 728, 935, 1092, 1217 |
| AsuC2I | CCSGG | 2 cut(s) | 249, 569 |
| AsuHPI | GGTGA | 2 cut(s) | 104, 277 |
| AvaII | GGWCC | 5 cut(s) | 63, 663, 718, 1105, 1176 |
| BaeGI | GKGCMC | 3 cut(s) | 74, 1192, 1241 |
| BanI | GGYRCC | 4 cut(s) | 71, 656, 725, 1033 |
| BanII | GRGCYC | 4 cut(s) | 708, 987, 1192, 1241 |
| BauI | CACGAG | 1 cut(s) | 1085 |
| BbsI | GAAGAC | 1 cut(s) | 200 |
| Bbv12I | GWGCWC | 2 cut(s) | 88, 987 |
| BbvI | GCAGC | 4 cut(s) | 43, 331, 534, 1047 |
| BccI | CCATC | 9 cut(s) | 200, 318, 440, 446, 455, 543, 737, 944, 1132 |
| BceAI | ACGGC | 4 cut(s) | 69, 236, 693, 706 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 38, 61, 72, 95 |
| BciT130I | CCWGG | 7 cut(s) | 67, 661, 722, 781, 888, 920, 1163 |
| BcnI | CCSGG | 2 cut(s) | 249, 569 |
| BcoDI | GTCTC | 2 cut(s) | 95, 246 |
| BfaI | CTAG | 2 cut(s) | 98, 230 |
| BfmI | CTRYAG | 1 cut(s) | 276 |
| BfoI | RGCGCY | 3 cut(s) | 528, 729, 1093 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1017 |
| BisI | GCNGC | 7 cut(s) | 57, 345, 523, 694, 931, 963, 1061 |
| BlpI | GCTNAGC | 1 cut(s) | 1146 |
| BlsI | GCNGC | 7 cut(s) | 58, 346, 524, 695, 932, 964, 1062 |
| Bme1390I | CCNGG | 9 cut(s) | 67, 249, 569, 661, 722, 781, 888, 920, 1163 |
| Bme18I | GGWCC | 5 cut(s) | 63, 663, 718, 1105, 1176 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 716 |
| BmiI | GGNNCC | 9 cut(s) | 6, 73, 658, 727, 1035, 1106, 1190, 1191, 1239 |
| BmrFI | CCNGG | 9 cut(s) | 67, 249, 569, 661, 722, 781, 888, 920, 1163 |
| BmsI | GCATC | 1 cut(s) | 178 |
| BpiI | GAAGAC | 1 cut(s) | 200 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 397, 429 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1146 |
| BpuEI | CTTGAG | 1 cut(s) | 1052 |
| BpuMI | CCSGG | 2 cut(s) | 249, 569 |
| Bsa29I | ATCGAT | 2 cut(s) | 27, 450 |
| BsaHI | GRCGYC | 1 cut(s) | 726 |
| BsaI | GGTCTC | 1 cut(s) | 95 |
| BsaJI | CCNNGG | 8 cut(s) | 66, 504, 721, 754, 779, 780, 886, 966 |
| BsaWI | WCCGGW | 3 cut(s) | 584, 715, 954 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 108, 138 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 323, 452, 722, 785, 1043, 1185 |
| Bse118I | RCCGGY | 2 cut(s) | 715, 807 |
| Bse1I | ACTGG | 3 cut(s) | 75, 442, 658 |
| Bse3DI | GCAATG | 1 cut(s) | 1037 |
| BseAI | TCCGGA | 1 cut(s) | 954 |
| BseBI | CCWGG | 7 cut(s) | 67, 661, 722, 781, 888, 920, 1163 |
| BseCI | ATCGAT | 2 cut(s) | 27, 450 |
| BseDI | CCNNGG | 8 cut(s) | 66, 504, 721, 754, 779, 780, 886, 966 |
| BseGI | GGATG | 1 cut(s) | 423 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 323, 452, 722, 785, 1043, 1185 |
| BseMI | GCAATG | 1 cut(s) | 1037 |
| BseMII | CTCAG | 3 cut(s) | 774, 988, 1160 |
| BseNI | ACTGG | 3 cut(s) | 75, 442, 658 |
| BseRI | GAGGAG | 1 cut(s) | 628 |
| BseSI | GKGCMC | 3 cut(s) | 74, 1192, 1241 |
| BseX3I | CGGCCG | 1 cut(s) | 691 |
| BseXI | GCAGC | 4 cut(s) | 43, 331, 534, 1047 |
| BseYI | CCCAGC | 3 cut(s) | 160, 510, 1042 |
| BsgI | GTGCAG | 1 cut(s) | 616 |
| Bsh1236I | CGCG | 3 cut(s) | 321, 933, 1215 |
| Bsh1285I | CGRYCG | 3 cut(s) | 265, 585, 694 |
| BshFI | GGCC | 9 cut(s) | 159, 223, 441, 509, 680, 693, 1020, 1190, 1239 |
| BshNI | GGYRCC | 4 cut(s) | 71, 656, 725, 1033 |
| BshTI | ACCGGT | 1 cut(s) | 715 |
| BshVI | ATCGAT | 2 cut(s) | 27, 450 |
| BsiEI | CGRYCG | 3 cut(s) | 265, 585, 694 |
| BsiHKAI | GWGCWC | 2 cut(s) | 88, 987 |
| BsiSI | CCGG | 6 cut(s) | 249, 568, 585, 716, 808, 955 |
| BslFI | GGGAC | 1 cut(s) | 1118 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 323, 452, 722, 785, 1043, 1185 |
| BsmAI | GTCTC | 2 cut(s) | 95, 246 |
| BsmFI | GGGAC | 1 cut(s) | 1118 |
| BsmI | GAATGC | 1 cut(s) | 277 |
| BsnI | GGCC | 9 cut(s) | 159, 223, 441, 509, 680, 693, 1020, 1190, 1239 |
| Bso31I | GGTCTC | 1 cut(s) | 95 |
| Bsp120I | GGGCCC | 2 cut(s) | 1188, 1237 |
| Bsp1286I | GDGCHC | 6 cut(s) | 74, 88, 708, 987, 1192, 1241 |
| Bsp13I | TCCGGA | 1 cut(s) | 954 |
| Bsp143I | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1146 |
| Bsp19I | CCATGG | 2 cut(s) | 504, 754 |
| BspACI | CCGC | 9 cut(s) | 167, 261, 321, 369, 694, 928, 931, 963, 1009 |
| BspANI | GGCC | 9 cut(s) | 159, 223, 441, 509, 680, 693, 1020, 1190, 1239 |
| BspCNI | CTCAG | 3 cut(s) | 773, 987, 1159 |
| BspDI | ATCGAT | 2 cut(s) | 27, 450 |
| BspEI | TCCGGA | 1 cut(s) | 954 |
| BspFNI | CGCG | 3 cut(s) | 321, 933, 1215 |
| BspLI | GGNNCC | 9 cut(s) | 6, 73, 658, 727, 1035, 1106, 1190, 1191, 1239 |
| BspPI | GGATC | 3 cut(s) | 770, 887, 965 |
| BspQI | GCTCTTC | 1 cut(s) | 1059 |
| BspT107I | GGYRCC | 4 cut(s) | 71, 656, 725, 1033 |
| BspTNI | GGTCTC | 1 cut(s) | 95 |
| BsrDI | GCAATG | 1 cut(s) | 1037 |
| BsrFI | RCCGGY | 2 cut(s) | 715, 807 |
| BsrI | ACTGG | 3 cut(s) | 75, 442, 658 |
| BssAI | RCCGGY | 2 cut(s) | 715, 807 |
| BssECI | CCNNGG | 8 cut(s) | 66, 504, 721, 754, 779, 780, 886, 966 |
| BssMI | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| BssNI | GRCGYC | 1 cut(s) | 726 |
| BssSI | CACGAG | 1 cut(s) | 1085 |
| BssT1I | CCWWGG | 2 cut(s) | 504, 754 |
| Bst2BI | CACGAG | 1 cut(s) | 1085 |
| Bst2UI | CCWGG | 7 cut(s) | 67, 661, 722, 781, 888, 920, 1163 |
| Bst4CI | ACNGT | 2 cut(s) | 206, 833 |
| Bst6I | CTCTTC | 1 cut(s) | 1059 |
| BstACI | GRCGYC | 1 cut(s) | 726 |
| BstC8I | GCNNGC | 8 cut(s) | 335, 458, 555, 647, 740, 916, 1009, 1213 |
| BstDEI | CTNAG | 4 cut(s) | 12, 760, 974, 1146 |
| BstDSI | CCRYGG | 2 cut(s) | 504, 754 |
| BstF5I | GGATG | 1 cut(s) | 423 |
| BstFNI | CGCG | 3 cut(s) | 321, 933, 1215 |
| BstH2I | RGCGCY | 3 cut(s) | 528, 729, 1093 |
| BstHHI | GCGC | 5 cut(s) | 527, 728, 935, 1092, 1217 |
| BstKTI | GATC | 6 cut(s) | 31, 142, 301, 778, 895, 960 |
| BstMAI | GTCTC | 2 cut(s) | 95, 246 |
| BstMBI | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| BstMCI | CGRYCG | 3 cut(s) | 265, 585, 694 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 466, 920, 1017, 1087 |
| BstNI | CCWGG | 7 cut(s) | 67, 661, 722, 781, 888, 920, 1163 |
| BstNSI | RCATGY | 2 cut(s) | 742, 826 |
| BstSCI | CCNGG | 9 cut(s) | 65, 247, 567, 659, 720, 779, 886, 918, 1161 |
| BstSFI | CTRYAG | 1 cut(s) | 276 |
| BstSLI | GKGCMC | 3 cut(s) | 74, 1192, 1241 |
| BstUI | CGCG | 3 cut(s) | 321, 933, 1215 |
| BstV1I | GCAGC | 4 cut(s) | 43, 331, 534, 1047 |
| BstV2I | GAAGAC | 1 cut(s) | 200 |
| BstZI | CGGCCG | 1 cut(s) | 691 |
| Bsu15I | ATCGAT | 2 cut(s) | 27, 450 |
| BsuRI | GGCC | 9 cut(s) | 159, 223, 441, 509, 680, 693, 1020, 1190, 1239 |
| BsuTUI | ATCGAT | 2 cut(s) | 27, 450 |
| BtgI | CCRYGG | 2 cut(s) | 504, 754 |
| BtgZI | GCGATG | 1 cut(s) | 178 |
| BtsCI | GGATG | 1 cut(s) | 423 |
| BtsIMutI | CAGTG | 3 cut(s) | 435, 651, 1133 |
| Cac8I | GCNNGC | 8 cut(s) | 335, 458, 555, 647, 740, 916, 1009, 1213 |
| CfoI | GCGC | 5 cut(s) | 527, 728, 935, 1092, 1217 |
| Cfr10I | RCCGGY | 2 cut(s) | 715, 807 |
| ClaI | ATCGAT | 2 cut(s) | 27, 450 |
| Csp6I | GTAC | 4 cut(s) | 202, 866, 1034, 1207 |
| CspAI | ACCGGT | 1 cut(s) | 715 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1082, 1117 |
| CviAII | CATG | 7 cut(s) | 199, 470, 505, 739, 755, 770, 823 |
| CviQI | GTAC | 4 cut(s) | 202, 866, 1034, 1207 |
| DdeI | CTNAG | 4 cut(s) | 12, 760, 974, 1146 |
| DinI | GGCGCC | 1 cut(s) | 727 |
| DpnI | GATC | 6 cut(s) | 30, 141, 300, 777, 894, 959 |
| DpnII | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| DriI | GACNNNNNGTC | 1 cut(s) | 716 |
| EaeI | YGGCCR | 1 cut(s) | 691 |
| EagI | CGGCCG | 1 cut(s) | 691 |
| Eam1104I | CTCTTC | 1 cut(s) | 1059 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 716 |
| EarI | CTCTTC | 1 cut(s) | 1059 |
| EciI | GGCGGA | 1 cut(s) | 917 |
| Ecl136II | GAGCTC | 1 cut(s) | 985 |
| EclXI | CGGCCG | 1 cut(s) | 691 |
| Eco130I | CCWWGG | 2 cut(s) | 504, 754 |
| Eco24I | GRGCYC | 4 cut(s) | 708, 987, 1192, 1241 |
| Eco31I | GGTCTC | 1 cut(s) | 95 |
| Eco32I | GATATC | 1 cut(s) | 952 |
| Eco47I | GGWCC | 5 cut(s) | 63, 663, 718, 1105, 1176 |
| Eco47III | AGCGCT | 1 cut(s) | 526 |
| Eco52I | CGGCCG | 1 cut(s) | 691 |
| Eco53kI | GAGCTC | 1 cut(s) | 985 |
| Eco57I | CTGAAG | 2 cut(s) | 373, 1040 |
| EcoICRI | GAGCTC | 1 cut(s) | 985 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1105, 1189 |
| EcoRII | CCWGG | 7 cut(s) | 65, 659, 720, 779, 886, 918, 1161 |
| EcoRV | GATATC | 1 cut(s) | 952 |
| EcoT14I | CCWWGG | 2 cut(s) | 504, 754 |
| EcoT38I | GRGCYC | 4 cut(s) | 708, 987, 1192, 1241 |
| EgeI | GGCGCC | 1 cut(s) | 727 |
| EheI | GGCGCC | 1 cut(s) | 727 |
| ErhI | CCWWGG | 2 cut(s) | 504, 754 |
| FaeI | CATG | 7 cut(s) | 202, 473, 508, 742, 758, 773, 826 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 844, 876 |
| FaqI | GGGAC | 1 cut(s) | 1118 |
| FatI | CATG | 7 cut(s) | 198, 469, 504, 738, 754, 769, 822 |
| FauI | CCCGC | 1 cut(s) | 1016 |
| FauNDI | CATATG | 1 cut(s) | 463 |
| FblI | GTMKAC | 1 cut(s) | 1222 |
| Fnu4HI | GCNGC | 7 cut(s) | 57, 345, 523, 694, 931, 963, 1061 |
| FokI | GGATG | 1 cut(s) | 410 |
| FriOI | GRGCYC | 4 cut(s) | 708, 987, 1192, 1241 |
| Fsp4HI | GCNGC | 7 cut(s) | 57, 345, 523, 694, 931, 963, 1061 |
| FspBI | CTAG | 2 cut(s) | 98, 230 |
| GlaI | GCGC | 5 cut(s) | 526, 727, 934, 1091, 1216 |
| GluI | GCNGC | 7 cut(s) | 57, 345, 523, 694, 931, 963, 1061 |
| GsaI | CCCAGC | 3 cut(s) | 164, 514, 1046 |
| HaeII | RGCGCY | 3 cut(s) | 528, 729, 1093 |
| HaeIII | GGCC | 9 cut(s) | 159, 223, 441, 509, 680, 693, 1020, 1190, 1239 |
| HapII | CCGG | 6 cut(s) | 249, 568, 585, 716, 808, 955 |
| HhaI | GCGC | 5 cut(s) | 527, 728, 935, 1092, 1217 |
| Hin1I | GRCGYC | 1 cut(s) | 726 |
| Hin1II | CATG | 7 cut(s) | 202, 473, 508, 742, 758, 773, 826 |
| Hin6I | GCGC | 5 cut(s) | 525, 726, 933, 1090, 1215 |
| HinP1I | GCGC | 5 cut(s) | 525, 726, 933, 1090, 1215 |
| HincII | GTYRAC | 1 cut(s) | 1223 |
| HindII | GTYRAC | 1 cut(s) | 1223 |
| HinfI | GANTC | 4 cut(s) | 244, 482, 855, 1171 |
| HpaII | CCGG | 6 cut(s) | 249, 568, 585, 716, 808, 955 |
| HphI | GGTGA | 2 cut(s) | 104, 277 |
| Hpy166II | GTNNAC | 2 cut(s) | 1179, 1223 |
| Hpy188I | TCNGA | 9 cut(s) | 63, 139, 218, 239, 392, 710, 763, 907, 949 |
| Hpy188III | TCNNGA | 6 cut(s) | 32, 98, 361, 955, 1085, 1230 |
| Hpy8I | GTNNAC | 2 cut(s) | 1179, 1223 |
| Hpy99I | CGWCG | 1 cut(s) | 222 |
| HpyAV | CCTTC | 8 cut(s) | 137, 234, 452, 607, 779, 870, 1042, 1236 |
| HpyCH4III | ACNGT | 2 cut(s) | 206, 833 |
| HpyCH4V | TGCA | 4 cut(s) | 469, 522, 633, 645 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 466, 920, 1017, 1087 |
| HpyF3I | CTNAG | 4 cut(s) | 12, 760, 974, 1146 |
| Hsp92I | GRCGYC | 1 cut(s) | 726 |
| Hsp92II | CATG | 7 cut(s) | 202, 473, 508, 742, 758, 773, 826 |
| HspAI | GCGC | 5 cut(s) | 525, 726, 933, 1090, 1215 |
| KasI | GGCGCC | 1 cut(s) | 725 |
| Kpn2I | TCCGGA | 1 cut(s) | 954 |
| KpnI | GGTACC | 1 cut(s) | 1037 |
| Kzo9I | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| LguI | GCTCTTC | 1 cut(s) | 1059 |
| LmnI | GCTCC | 2 cut(s) | 10, 997 |
| Lsp1109I | GCAGC | 4 cut(s) | 43, 331, 534, 1047 |
| LweI | GCATC | 1 cut(s) | 178 |
| MaeI | CTAG | 2 cut(s) | 98, 230 |
| MalI | GATC | 6 cut(s) | 30, 141, 300, 777, 894, 959 |
| MboI | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| MboII | GAAGA | 9 cut(s) | 47, 50, 200, 305, 861, 864, 1046, 1108, 1180 |
| MfeI | CAATTG | 2 cut(s) | 1028, 1076 |
| MhlI | GDGCHC | 6 cut(s) | 74, 88, 708, 987, 1192, 1241 |
| MluCI | AATT | 8 cut(s) | 41, 476, 591, 620, 840, 1013, 1028, 1076 |
| Mly113I | GGCGCC | 1 cut(s) | 726 |
| MlyI | GAGTC | 2 cut(s) | 238, 476 |
| MmeI | TCCRAC | 3 cut(s) | 241, 399, 930 |
| MroI | TCCGGA | 1 cut(s) | 954 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1115 |
| MseI | TTAA | 1 cut(s) | 839 |
| MslI | CAYNNNNRTG | 1 cut(s) | 197 |
| MspA1I | CMGCKG | 1 cut(s) | 1060 |
| MspI | CCGG | 6 cut(s) | 249, 568, 585, 716, 808, 955 |
| MspR9I | CCNGG | 9 cut(s) | 67, 249, 569, 661, 722, 781, 888, 920, 1163 |
| MunI | CAATTG | 2 cut(s) | 1028, 1076 |
| Mva1269I | GAATGC | 1 cut(s) | 277 |
| MvaI | CCWGG | 7 cut(s) | 67, 661, 722, 781, 888, 920, 1163 |
| MvnI | CGCG | 3 cut(s) | 321, 933, 1215 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 466, 920, 1017, 1087 |
| NarI | GGCGCC | 1 cut(s) | 726 |
| NciI | CCSGG | 2 cut(s) | 249, 569 |
| NcoI | CCATGG | 2 cut(s) | 504, 754 |
| NdeI | CATATG | 1 cut(s) | 463 |
| NdeII | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| NlaIII | CATG | 7 cut(s) | 202, 473, 508, 742, 758, 773, 826 |
| NlaIV | GGNNCC | 9 cut(s) | 6, 73, 658, 727, 1035, 1106, 1190, 1191, 1239 |
| NmeAIII | GCCGAG | 1 cut(s) | 991 |
| NspI | RCATGY | 2 cut(s) | 742, 826 |
| PaeI | GCATGC | 1 cut(s) | 742 |
| PasI | CCCWGGG | 1 cut(s) | 780 |
| PciSI | GCTCTTC | 1 cut(s) | 1059 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1219, 1228 |
| PctI | GAATGC | 1 cut(s) | 277 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1115 |
| PfeI | GAWTC | 2 cut(s) | 855, 1171 |
| PfoI | TCCNGGA | 1 cut(s) | 247 |
| PinAI | ACCGGT | 1 cut(s) | 715 |
| PkrI | GCNGC | 7 cut(s) | 58, 346, 524, 695, 932, 964, 1062 |
| PleI | GAGTC | 2 cut(s) | 238, 476 |
| PluTI | GGCGCC | 1 cut(s) | 729 |
| PpsI | GAGTC | 2 cut(s) | 238, 476 |
| PpuMI | RGGWCCY | 1 cut(s) | 1105 |
| Psp124BI | GAGCTC | 1 cut(s) | 987 |
| Psp5II | RGGWCCY | 1 cut(s) | 1105 |
| Psp6I | CCWGG | 7 cut(s) | 65, 659, 720, 779, 886, 918, 1161 |
| PspFI | CCCAGC | 3 cut(s) | 160, 510, 1042 |
| PspGI | CCWGG | 7 cut(s) | 65, 659, 720, 779, 886, 918, 1161 |
| PspN4I | GGNNCC | 9 cut(s) | 6, 73, 658, 727, 1035, 1106, 1190, 1191, 1239 |
| PspOMI | GGGCCC | 2 cut(s) | 1188, 1237 |
| PspPPI | RGGWCCY | 1 cut(s) | 1105 |
| PvuII | CAGCTG | 1 cut(s) | 1060 |
| RsaI | GTAC | 4 cut(s) | 203, 867, 1035, 1208 |
| RsaNI | GTAC | 4 cut(s) | 202, 866, 1034, 1207 |
| RseI | CAYNNNNRTG | 1 cut(s) | 197 |
| SacI | GAGCTC | 1 cut(s) | 987 |
| SalI | GTCGAC | 1 cut(s) | 1221 |
| SapI | GCTCTTC | 1 cut(s) | 1059 |
| SaqAI | TTAA | 1 cut(s) | 839 |
| SatI | GCNGC | 7 cut(s) | 57, 345, 523, 694, 931, 963, 1061 |
| Sau3AI | GATC | 6 cut(s) | 28, 139, 298, 775, 892, 957 |
| SchI | GAGTC | 2 cut(s) | 238, 476 |
| ScrFI | CCNGG | 9 cut(s) | 67, 249, 569, 661, 722, 781, 888, 920, 1163 |
| SduI | GDGCHC | 6 cut(s) | 74, 88, 708, 987, 1192, 1241 |
| SfaNI | GCATC | 1 cut(s) | 178 |
| SfcI | CTRYAG | 1 cut(s) | 276 |
| SfoI | GGCGCC | 1 cut(s) | 727 |
| SinI | GGWCC | 5 cut(s) | 63, 663, 718, 1105, 1176 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 197 |
| SmlI | CTYRAG | 1 cut(s) | 1067 |
| SmoI | CTYRAG | 1 cut(s) | 1067 |
| SphI | GCATGC | 1 cut(s) | 742 |
| Sse9I | AATT | 8 cut(s) | 41, 476, 591, 620, 840, 1013, 1028, 1076 |
| SsiI | CCGC | 9 cut(s) | 167, 261, 321, 369, 694, 928, 931, 963, 1009 |
| SspDI | GGCGCC | 1 cut(s) | 725 |
| SspI | AATATT | 1 cut(s) | 608 |
| SspMI | CTAG | 2 cut(s) | 98, 230 |
| SstI | GAGCTC | 1 cut(s) | 987 |
| StyD4I | CCNGG | 9 cut(s) | 65, 247, 567, 659, 720, 779, 886, 918, 1161 |
| StyI | CCWWGG | 2 cut(s) | 504, 754 |
| TaaI | ACNGT | 2 cut(s) | 206, 833 |
| TaqI | TCGA | 3 cut(s) | 27, 450, 1222 |
| TaqII | GACCGA | 1 cut(s) | 1164 |
| TasI | AATT | 8 cut(s) | 41, 476, 591, 620, 840, 1013, 1028, 1076 |
| TatI | WGTACW | 1 cut(s) | 865 |
| TauI | GCSGC | 3 cut(s) | 696, 933, 965 |
| TfiI | GAWTC | 2 cut(s) | 855, 1171 |
| Tru1I | TTAA | 1 cut(s) | 839 |
| Tru9I | TTAA | 1 cut(s) | 839 |
| TscAI | CASTG | 3 cut(s) | 442, 658, 1140 |
| TseI | GCWGC | 4 cut(s) | 56, 344, 522, 1060 |
| TspDTI | ATGAA | 4 cut(s) | 83, 528, 613, 807 |
| TspRI | CASTG | 3 cut(s) | 442, 658, 1140 |
| VpaK11BI | GGWCC | 5 cut(s) | 63, 663, 718, 1105, 1176 |
| XapI | RAATTY | 1 cut(s) | 620 |
| XbaI | TCTAGA | 1 cut(s) | 97 |
| XceI | RCATGY | 2 cut(s) | 742, 826 |
| XmiI | GTMKAC | 1 cut(s) | 1222 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1115 |
| XspI | CTAG | 2 cut(s) | 98, 230 |