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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 543 |
| AarI | CACCTGC | 1 cut(s) | 1375 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1122, 1773 |
| AatII | GACGTC | 1 cut(s) | 1320 |
| Acc36I | ACCTGC | 6 cut(s) | 371, 1119, 1375, 1543, 1552, 2273 |
| Acc65I | GGTACC | 1 cut(s) | 511 |
| AccB1I | GGYRCC | 2 cut(s) | 71, 511 |
| AccI | GTMKAC | 2 cut(s) | 612, 648 |
| AclWI | GGATC | 4 cut(s) | 1076, 1496, 2034, 2042 |
| AcoI | YGGCCR | 4 cut(s) | 302, 913, 939, 1920 |
| AcsI | RAATTY | 2 cut(s) | 1300, 1862 |
| AcuI | CTGAAG | 4 cut(s) | 816, 999, 1683, 1875 |
| AcyI | GRCGYC | 2 cut(s) | 642, 1317 |
| AfaI | GTAC | 5 cut(s) | 513, 1263, 1713, 1830, 1846 |
| AfeI | AGCGCT | 2 cut(s) | 1356, 2013 |
| AgsI | TTSAA | 5 cut(s) | 46, 1394, 1652, 1871, 2066 |
| AhlI | ACTAGT | 1 cut(s) | 2083 |
| AjnI | CCWGG | 9 cut(s) | 65, 321, 533, 551, 858, 910, 1085, 1729, 1792 |
| Alw21I | GWGCWC | 4 cut(s) | 750, 783, 1813, 1884 |
| Alw26I | GTCTC | 5 cut(s) | 95, 320, 694, 1283, 2184 |
| AlwI | GGATC | 4 cut(s) | 1076, 1496, 2034, 2042 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 264, 1631 |
| Aor51HI | AGCGCT | 2 cut(s) | 1356, 2013 |
| ApaI | GGGCCC | 2 cut(s) | 582, 1430 |
| ApoI | RAATTY | 2 cut(s) | 1300, 1862 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 710, 742 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1215 |
| Asp718I | GGTACC | 1 cut(s) | 511 |
| AspA2I | CCTAGG | 1 cut(s) | 1999 |
| AsuC2I | CCSGG | 3 cut(s) | 61, 151, 249 |
| AsuHPI | GGTGA | 5 cut(s) | 104, 379, 437, 1356, 2015 |
| AsuNHI | GCTAGC | 1 cut(s) | 2240 |
| AvaII | GGWCC | 7 cut(s) | 63, 654, 994, 1205, 1330, 1775, 2055 |
| AvrII | CCTAGG | 1 cut(s) | 1999 |
| BaeGI | GKGCMC | 4 cut(s) | 74, 322, 582, 1430 |
| BalI | TGGCCA | 1 cut(s) | 304 |
| BanI | GGYRCC | 2 cut(s) | 71, 511 |
| BanII | GRGCYC | 8 cut(s) | 582, 631, 750, 1430, 1620, 1813, 1884, 2142 |
| BauI | CACGAG | 1 cut(s) | 1358 |
| BbsI | GAAGAC | 2 cut(s) | 200, 1530 |
| Bbv12I | GWGCWC | 4 cut(s) | 750, 783, 1813, 1884 |
| BbvCI | CCTCAGC | 3 cut(s) | 444, 990, 1482 |
| BccI | CCATC | 8 cut(s) | 288, 313, 404, 420, 524, 1232, 1819, 1856 |
| BceAI | ACGGC | 5 cut(s) | 236, 629, 1935, 2133, 2178 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 38, 72, 2249, 2283 |
| BciT130I | CCWGG | 9 cut(s) | 67, 323, 535, 553, 860, 912, 1087, 1731, 1794 |
| BclI | TGATCA | 1 cut(s) | 2005 |
| BcnI | CCSGG | 3 cut(s) | 61, 151, 249 |
| BcoDI | GTCTC | 5 cut(s) | 95, 320, 694, 1283, 2184 |
| BcuI | ACTAGT | 1 cut(s) | 2083 |
| BfaI | CTAG | 8 cut(s) | 98, 362, 1715, 2000, 2084, 2168, 2241, 2252 |
| BfmI | CTRYAG | 3 cut(s) | 378, 984, 1044 |
| BfoI | RGCGCY | 5 cut(s) | 87, 1193, 1358, 2015, 2168 |
| BfuAI | ACCTGC | 6 cut(s) | 371, 1119, 1375, 1543, 1552, 2273 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 495, 2126 |
| BglII | AGATCT | 1 cut(s) | 358 |
| BlnI | CCTAGG | 1 cut(s) | 1999 |
| Bme18I | GGWCC | 7 cut(s) | 63, 654, 994, 1205, 1330, 1775, 2055 |
| BmrI | ACTGGG | 1 cut(s) | 963 |
| BmsI | GCATC | 5 cut(s) | 178, 520, 790, 876, 2227 |
| BmtI | GCTAGC | 1 cut(s) | 2244 |
| BmuI | ACTGGG | 1 cut(s) | 963 |
| BpiI | GAAGAC | 2 cut(s) | 200, 1530 |
| BpmI | CTGGAG | 1 cut(s) | 1713 |
| Bpu10I | CCTNAGC | 4 cut(s) | 444, 749, 990, 1482 |
| BpuEI | CTTGAG | 4 cut(s) | 873, 1152, 1797, 2178 |
| BpuMI | CCSGG | 3 cut(s) | 61, 151, 249 |
| Bsa29I | ATCGAT | 2 cut(s) | 27, 399 |
| BsaHI | GRCGYC | 2 cut(s) | 642, 1317 |
| BsaI | GGTCTC | 2 cut(s) | 95, 320 |
| BsaWI | WCCGGW | 1 cut(s) | 1258 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 26, 56, 108, 138, 829, 859 |
| Bse118I | RCCGGY | 4 cut(s) | 586, 863, 937, 1472 |
| Bse1I | ACTGG | 4 cut(s) | 75, 958, 1122, 2130 |
| Bse3DI | GCAATG | 1 cut(s) | 192 |
| BseBI | CCWGG | 9 cut(s) | 67, 323, 535, 553, 860, 912, 1087, 1731, 1794 |
| BseCI | ATCGAT | 2 cut(s) | 27, 399 |
| BseGI | GGATG | 4 cut(s) | 305, 516, 1413, 1747 |
| BseMI | GCAATG | 1 cut(s) | 192 |
| BseMII | CTCAG | 8 cut(s) | 435, 466, 763, 981, 1080, 1473, 2021, 2195 |
| BseNI | ACTGG | 4 cut(s) | 75, 958, 1122, 2130 |
| BsePI | GCGCGC | 2 cut(s) | 2198, 2224 |
| BseRI | GAGGAG | 5 cut(s) | 837, 1472, 1662, 1716, 2150 |
| BseSI | GKGCMC | 4 cut(s) | 74, 322, 582, 1430 |
| BseX3I | CGGCCG | 2 cut(s) | 939, 1920 |
| BseYI | CCCAGC | 4 cut(s) | 160, 264, 1145, 1621 |
| BsgI | GTGCAG | 1 cut(s) | 1914 |
| Bsh1285I | CGRYCG | 3 cut(s) | 942, 1611, 1923 |
| BshNI | GGYRCC | 2 cut(s) | 71, 511 |
| BshVI | ATCGAT | 2 cut(s) | 27, 399 |
| BsiEI | CGRYCG | 3 cut(s) | 942, 1611, 1923 |
| BsiHKAI | GWGCWC | 4 cut(s) | 750, 783, 1813, 1884 |
| BsiSI | CCGG | 8 cut(s) | 61, 150, 249, 587, 864, 938, 1259, 1473 |
| BslFI | GGGAC | 5 cut(s) | 667, 1218, 1304, 1433, 1766 |
| BsmAI | GTCTC | 5 cut(s) | 95, 320, 694, 1283, 2184 |
| BsmBI | CGTCTC | 1 cut(s) | 1283 |
| BsmFI | GGGAC | 5 cut(s) | 667, 1218, 1304, 1433, 1766 |
| BsmI | GAATGC | 1 cut(s) | 379 |
| Bso31I | GGTCTC | 2 cut(s) | 95, 320 |
| Bsp120I | GGGCCC | 2 cut(s) | 578, 1426 |
| Bsp68I | TCGCGA | 2 cut(s) | 342, 1186 |
| BspCNI | CTCAG | 8 cut(s) | 436, 465, 762, 982, 1079, 1474, 2022, 2194 |
| BspDI | ATCGAT | 2 cut(s) | 27, 399 |
| BspMAI | CTGCAG | 2 cut(s) | 382, 988 |
| BspMI | ACCTGC | 6 cut(s) | 371, 1119, 1375, 1543, 1552, 2273 |
| BspOI | GCTAGC | 1 cut(s) | 2244 |
| BspPI | GGATC | 4 cut(s) | 1076, 1496, 2034, 2042 |
| BspQI | GCTCTTC | 1 cut(s) | 1162 |
| BspT107I | GGYRCC | 2 cut(s) | 71, 511 |
| BspTNI | GGTCTC | 2 cut(s) | 95, 320 |
| BsrDI | GCAATG | 1 cut(s) | 192 |
| BsrFI | RCCGGY | 4 cut(s) | 586, 863, 937, 1472 |
| BsrI | ACTGG | 4 cut(s) | 75, 958, 1122, 2130 |
| BssAI | RCCGGY | 4 cut(s) | 586, 863, 937, 1472 |
| BssHII | GCGCGC | 2 cut(s) | 2198, 2224 |
| BssNI | GRCGYC | 2 cut(s) | 642, 1317 |
| BssSI | CACGAG | 1 cut(s) | 1358 |
| BssT1I | CCWWGG | 2 cut(s) | 1999, 2094 |
| Bst2BI | CACGAG | 1 cut(s) | 1358 |
| Bst2UI | CCWGG | 9 cut(s) | 67, 323, 535, 553, 860, 912, 1087, 1731, 1794 |
| Bst4CI | ACNGT | 5 cut(s) | 929, 1033, 1935, 2113, 2209 |
| Bst6I | CTCTTC | 4 cut(s) | 33, 731, 1162, 1290 |
| BstACI | GRCGYC | 2 cut(s) | 642, 1317 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 974, 1631 |
| BstDEI | CTNAG | 9 cut(s) | 12, 444, 452, 749, 990, 1066, 1482, 2030, 2181 |
| BstDSI | CCRYGG | 1 cut(s) | 1923 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 891, 1481 |
| BstF5I | GGATG | 4 cut(s) | 305, 516, 1413, 1747 |
| BstH2I | RGCGCY | 5 cut(s) | 87, 1193, 1358, 2015, 2168 |
| BstMAI | GTCTC | 5 cut(s) | 95, 320, 694, 1283, 2184 |
| BstMCI | CGRYCG | 3 cut(s) | 942, 1611, 1923 |
| BstNI | CCWGG | 9 cut(s) | 67, 323, 535, 553, 860, 912, 1087, 1731, 1794 |
| BstSFI | CTRYAG | 3 cut(s) | 378, 984, 1044 |
| BstSLI | GKGCMC | 4 cut(s) | 74, 322, 582, 1430 |
| BstV2I | GAAGAC | 2 cut(s) | 200, 1530 |
| BstX2I | RGATCY | 3 cut(s) | 358, 2026, 2034 |
| BstYI | RGATCY | 3 cut(s) | 358, 2026, 2034 |
| BstZI | CGGCCG | 2 cut(s) | 939, 1920 |
| Bsu15I | ATCGAT | 2 cut(s) | 27, 399 |
| BsuTUI | ATCGAT | 2 cut(s) | 27, 399 |
| BtgI | CCRYGG | 1 cut(s) | 1923 |
| BtgZI | GCGATG | 2 cut(s) | 1642, 2189 |
| BtsCI | GGATG | 4 cut(s) | 305, 516, 1413, 1747 |
| BtsI | GCAGTG | 1 cut(s) | 975 |
| BtsIMutI | CAGTG | 4 cut(s) | 934, 975, 1233, 1851 |
| BtuMI | TCGCGA | 2 cut(s) | 342, 1186 |
| BveI | ACCTGC | 6 cut(s) | 371, 1119, 1375, 1543, 1552, 2273 |
| CaiI | CAGNNNCTG | 2 cut(s) | 264, 1631 |
| Cfr10I | RCCGGY | 4 cut(s) | 586, 863, 937, 1472 |
| Cfr42I | CCGCGG | 1 cut(s) | 1926 |
| ClaI | ATCGAT | 2 cut(s) | 27, 399 |
| CseI | GACGC | 1 cut(s) | 650 |
| Csp6I | GTAC | 5 cut(s) | 512, 1262, 1712, 1829, 1845 |
| CviAII | CATG | 4 cut(s) | 199, 1325, 1528, 2279 |
| CviQI | GTAC | 5 cut(s) | 512, 1262, 1712, 1829, 1845 |
| DdeI | CTNAG | 9 cut(s) | 12, 444, 452, 749, 990, 1066, 1482, 2030, 2181 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1122, 1773 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1122, 1773 |
| EaeI | YGGCCR | 4 cut(s) | 302, 913, 939, 1920 |
| EagI | CGGCCG | 2 cut(s) | 939, 1920 |
| Eam1104I | CTCTTC | 4 cut(s) | 33, 731, 1162, 1290 |
| EarI | CTCTTC | 4 cut(s) | 33, 731, 1162, 1290 |
| Ecl136II | GAGCTC | 3 cut(s) | 748, 1811, 1882 |
| EclXI | CGGCCG | 2 cut(s) | 939, 1920 |
| Eco130I | CCWWGG | 2 cut(s) | 1999, 2094 |
| Eco147I | AGGCCT | 2 cut(s) | 811, 1985 |
| Eco24I | GRGCYC | 8 cut(s) | 582, 631, 750, 1430, 1620, 1813, 1884, 2142 |
| Eco31I | GGTCTC | 2 cut(s) | 95, 320 |
| Eco47I | GGWCC | 7 cut(s) | 63, 654, 994, 1205, 1330, 1775, 2055 |
| Eco47III | AGCGCT | 2 cut(s) | 1356, 2013 |
| Eco52I | CGGCCG | 2 cut(s) | 939, 1920 |
| Eco53kI | GAGCTC | 3 cut(s) | 748, 1811, 1882 |
| Eco57I | CTGAAG | 4 cut(s) | 816, 999, 1683, 1875 |
| EcoICRI | GAGCTC | 3 cut(s) | 748, 1811, 1882 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 891, 1481 |
| EcoO109I | RGGNCCY | 7 cut(s) | 579, 1205, 1330, 1426, 1427, 1628, 1775 |
| EcoRI | GAATTC | 1 cut(s) | 1300 |
| EcoRII | CCWGG | 9 cut(s) | 65, 321, 533, 551, 858, 910, 1085, 1729, 1792 |
| EcoT14I | CCWWGG | 2 cut(s) | 1999, 2094 |
| EcoT38I | GRGCYC | 8 cut(s) | 582, 631, 750, 1430, 1620, 1813, 1884, 2142 |
| ErhI | CCWWGG | 2 cut(s) | 1999, 2094 |
| Esp3I | CGTCTC | 1 cut(s) | 1283 |
| FaeI | CATG | 4 cut(s) | 202, 1328, 1531, 2282 |
| FaqI | GGGAC | 5 cut(s) | 667, 1218, 1304, 1433, 1766 |
| FatI | CATG | 4 cut(s) | 198, 1324, 1527, 2278 |
| FauNDI | CATATG | 1 cut(s) | 1876 |
| FbaI | TGATCA | 1 cut(s) | 2005 |
| FblI | GTMKAC | 2 cut(s) | 612, 648 |
| FokI | GGATG | 4 cut(s) | 292, 503, 1400, 1754 |
| FriOI | GRGCYC | 8 cut(s) | 582, 631, 750, 1430, 1620, 1813, 1884, 2142 |
| FspBI | CTAG | 8 cut(s) | 98, 362, 1715, 2000, 2084, 2168, 2241, 2252 |
| GsaI | CCCAGC | 4 cut(s) | 164, 268, 1149, 1625 |
| GsuI | CTGGAG | 1 cut(s) | 1713 |
| HaeII | RGCGCY | 5 cut(s) | 87, 1193, 1358, 2015, 2168 |
| HapII | CCGG | 8 cut(s) | 61, 150, 249, 587, 864, 938, 1259, 1473 |
| HgaI | GACGC | 1 cut(s) | 650 |
| Hin1I | GRCGYC | 2 cut(s) | 642, 1317 |
| Hin1II | CATG | 4 cut(s) | 202, 1328, 1531, 2282 |
| HincII | GTYRAC | 2 cut(s) | 613, 697 |
| HindII | GTYRAC | 2 cut(s) | 613, 697 |
| HindIII | AAGCTT | 1 cut(s) | 2291 |
| HinfI | GANTC | 5 cut(s) | 244, 561, 706, 1767, 1994 |
| HpaII | CCGG | 8 cut(s) | 61, 150, 249, 587, 864, 938, 1259, 1473 |
| HphI | GGTGA | 5 cut(s) | 104, 379, 437, 1356, 2015 |
| Hpy166II | GTNNAC | 4 cut(s) | 613, 649, 697, 881 |
| Hpy8I | GTNNAC | 4 cut(s) | 613, 649, 697, 881 |
| Hpy99I | CGWCG | 4 cut(s) | 222, 1022, 1128, 1616 |
| HpyCH4III | ACNGT | 5 cut(s) | 929, 1033, 1935, 2113, 2209 |
| HpyCH4IV | ACGT | 2 cut(s) | 1317, 1843 |
| HpyCH4V | TGCA | 7 cut(s) | 380, 986, 1586, 1888, 1931, 1970, 2282 |
| HpyF3I | CTNAG | 9 cut(s) | 12, 444, 452, 749, 990, 1066, 1482, 2030, 2181 |
| HpySE526I | ACGT | 2 cut(s) | 1317, 1843 |
| Hsp92I | GRCGYC | 2 cut(s) | 642, 1317 |
| Hsp92II | CATG | 4 cut(s) | 202, 1328, 1531, 2282 |
| KpnI | GGTACC | 1 cut(s) | 515 |
| KroI | GCCGGC | 2 cut(s) | 863, 937 |
| KroNI | GCCGGC | 2 cut(s) | 865, 939 |
| Ksp22I | TGATCA | 1 cut(s) | 2005 |
| KspI | CCGCGG | 1 cut(s) | 1926 |
| LguI | GCTCTTC | 1 cut(s) | 1162 |
| LmnI | GCTCC | 5 cut(s) | 10, 475, 1729, 1887, 2137 |
| LweI | GCATC | 5 cut(s) | 178, 520, 790, 876, 2227 |
| MaeI | CTAG | 8 cut(s) | 98, 362, 1715, 2000, 2084, 2168, 2241, 2252 |
| MaeII | ACGT | 2 cut(s) | 1317, 1843 |
| MaeIII | GTNAC | 4 cut(s) | 1162, 1362, 2107, 2113 |
| MfeI | CAATTG | 1 cut(s) | 506 |
| MflI | RGATCY | 3 cut(s) | 358, 2026, 2034 |
| MlsI | TGGCCA | 1 cut(s) | 304 |
| MluCI | AATT | 7 cut(s) | 298, 491, 506, 538, 1300, 1862, 2102 |
| MluNI | TGGCCA | 1 cut(s) | 304 |
| MlyI | GAGTC | 4 cut(s) | 238, 700, 1761, 1988 |
| MmeI | TCCRAC | 1 cut(s) | 162 |
| Mox20I | TGGCCA | 1 cut(s) | 304 |
| MroNI | GCCGGC | 2 cut(s) | 863, 937 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1215 |
| MscI | TGGCCA | 1 cut(s) | 304 |
| MseI | TTAA | 2 cut(s) | 1908, 2159 |
| MslI | CAYNNNNRTG | 3 cut(s) | 197, 776, 2253 |
| Msp20I | TGGCCA | 1 cut(s) | 304 |
| MspA1I | CMGCKG | 8 cut(s) | 261, 443, 767, 944, 989, 1640, 1802, 1925 |
| MspI | CCGG | 8 cut(s) | 61, 150, 249, 587, 864, 938, 1259, 1473 |
| MunI | CAATTG | 1 cut(s) | 506 |
| Mva1269I | GAATGC | 1 cut(s) | 379 |
| MvaI | CCWGG | 9 cut(s) | 67, 323, 535, 553, 860, 912, 1087, 1731, 1794 |
| NaeI | GCCGGC | 2 cut(s) | 865, 939 |
| NciI | CCSGG | 3 cut(s) | 61, 151, 249 |
| NdeI | CATATG | 1 cut(s) | 1876 |
| NgoMIV | GCCGGC | 2 cut(s) | 863, 937 |
| NheI | GCTAGC | 1 cut(s) | 2240 |
| NlaIII | CATG | 4 cut(s) | 202, 1328, 1531, 2282 |
| NmeAIII | GCCGAG | 1 cut(s) | 1226 |
| NmuCI | GTSAC | 2 cut(s) | 1362, 2107 |
| NruI | TCGCGA | 2 cut(s) | 342, 1186 |
| PaqCI | CACCTGC | 1 cut(s) | 1375 |
| PasI | CCCWGGG | 1 cut(s) | 1086 |
| PauI | GCGCGC | 2 cut(s) | 2198, 2224 |
| PceI | AGGCCT | 2 cut(s) | 811, 1985 |
| PciSI | GCTCTTC | 1 cut(s) | 1162 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 609, 618, 1840 |
| PctI | GAATGC | 1 cut(s) | 379 |
| PdiI | GCCGGC | 2 cut(s) | 865, 939 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1215 |
| PfeI | GAWTC | 1 cut(s) | 561 |
| PfoI | TCCNGGA | 1 cut(s) | 247 |
| Ple19I | CGATCG | 1 cut(s) | 1611 |
| PleI | GAGTC | 4 cut(s) | 238, 700, 1761, 1988 |
| PpsI | GAGTC | 4 cut(s) | 238, 700, 1761, 1988 |
| PpuMI | RGGWCCY | 3 cut(s) | 1205, 1330, 1775 |
| PsiI | TTATAA | 1 cut(s) | 543 |
| Psp124BI | GAGCTC | 3 cut(s) | 750, 1813, 1884 |
| Psp5II | RGGWCCY | 3 cut(s) | 1205, 1330, 1775 |
| Psp6I | CCWGG | 9 cut(s) | 65, 321, 533, 551, 858, 910, 1085, 1729, 1792 |
| PspFI | CCCAGC | 4 cut(s) | 160, 264, 1145, 1621 |
| PspGI | CCWGG | 9 cut(s) | 65, 321, 533, 551, 858, 910, 1085, 1729, 1792 |
| PspOMI | GGGCCC | 2 cut(s) | 578, 1426 |
| PspPPI | RGGWCCY | 3 cut(s) | 1205, 1330, 1775 |
| PstI | CTGCAG | 2 cut(s) | 382, 988 |
| PstNI | CAGNNNCTG | 2 cut(s) | 264, 1631 |
| PsuI | RGATCY | 3 cut(s) | 358, 2026, 2034 |
| PteI | GCGCGC | 2 cut(s) | 2198, 2224 |
| PvuI | CGATCG | 1 cut(s) | 1611 |
| PvuII | CAGCTG | 2 cut(s) | 989, 1802 |
| RruI | TCGCGA | 2 cut(s) | 342, 1186 |
| RsaI | GTAC | 5 cut(s) | 513, 1263, 1713, 1830, 1846 |
| RsaNI | GTAC | 5 cut(s) | 512, 1262, 1712, 1829, 1845 |
| RseI | CAYNNNNRTG | 3 cut(s) | 197, 776, 2253 |
| SacI | GAGCTC | 3 cut(s) | 750, 1813, 1884 |
| SacII | CCGCGG | 1 cut(s) | 1926 |
| SalI | GTCGAC | 1 cut(s) | 611 |
| SapI | GCTCTTC | 1 cut(s) | 1162 |
| SaqAI | TTAA | 2 cut(s) | 1908, 2159 |
| SchI | GAGTC | 4 cut(s) | 238, 700, 1761, 1988 |
| SfaNI | GCATC | 5 cut(s) | 178, 520, 790, 876, 2227 |
| SfcI | CTRYAG | 3 cut(s) | 378, 984, 1044 |
| Sfr303I | CCGCGG | 1 cut(s) | 1926 |
| SgrBI | CCGCGG | 1 cut(s) | 1926 |
| SinI | GGWCC | 7 cut(s) | 63, 654, 994, 1205, 1330, 1775, 2055 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 197, 776, 2253 |
| SmlI | CTYRAG | 4 cut(s) | 888, 1167, 1812, 2193 |
| SmoI | CTYRAG | 4 cut(s) | 888, 1167, 1812, 2193 |
| SpeI | ACTAGT | 1 cut(s) | 2083 |
| Sse9I | AATT | 7 cut(s) | 298, 491, 506, 538, 1300, 1862, 2102 |
| SseBI | AGGCCT | 2 cut(s) | 811, 1985 |
| SspMI | CTAG | 8 cut(s) | 98, 362, 1715, 2000, 2084, 2168, 2241, 2252 |
| SstI | GAGCTC | 3 cut(s) | 750, 1813, 1884 |
| StuI | AGGCCT | 2 cut(s) | 811, 1985 |
| StyI | CCWWGG | 2 cut(s) | 1999, 2094 |
| TaaI | ACNGT | 5 cut(s) | 929, 1033, 1935, 2113, 2209 |
| TaiI | ACGT | 2 cut(s) | 1320, 1846 |
| TaqII | GACCGA | 2 cut(s) | 671, 1128 |
| TasI | AATT | 7 cut(s) | 298, 491, 506, 538, 1300, 1862, 2102 |
| TfiI | GAWTC | 1 cut(s) | 561 |
| Tru1I | TTAA | 2 cut(s) | 1908, 2159 |
| Tru9I | TTAA | 2 cut(s) | 1908, 2159 |
| TscAI | CASTG | 4 cut(s) | 934, 975, 1240, 1858 |
| TseFI | GTSAC | 2 cut(s) | 1362, 2107 |
| Tsp45I | GTSAC | 2 cut(s) | 1362, 2107 |
| TspDTI | ATGAA | 7 cut(s) | 83, 474, 1113, 1536, 1758, 1875, 2237 |
| TspGWI | ACGGA | 2 cut(s) | 866, 1307 |
| TspRI | CASTG | 4 cut(s) | 934, 975, 1240, 1858 |
| VpaK11BI | GGWCC | 7 cut(s) | 63, 654, 994, 1205, 1330, 1775, 2055 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 891, 1481 |
| XapI | RAATTY | 2 cut(s) | 1300, 1862 |
| XbaI | TCTAGA | 2 cut(s) | 97, 2251 |
| XmaJI | CCTAGG | 1 cut(s) | 1999 |
| XmiI | GTMKAC | 2 cut(s) | 612, 648 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1215 |
| XspI | CTAG | 8 cut(s) | 98, 362, 1715, 2000, 2084, 2168, 2241, 2252 |
| ZraI | GACGTC | 1 cut(s) | 1318 |